The present invention relates to the field of
RNA analysis. In particular, the invention concerns the use of a catalytic
nucleic acid molecule for the analysis of an
RNA molecule. The invention concerns methods for analyzing the 5′ terminal structures of an
RNA molecule having a cleavage site for a catalytic
nucleic acid molecule. In particular, the invention concerns a method for determining the presence of a cap structure in an
RNA molecule having a cleavage site for a catalytic
nucleic acid molecule, a method for determining the capping degree of a
population of RNA molecules having a cleavage site for a catalytic
nucleic acid molecule, a method for determining the orientation of the cap structure in a capped
RNA molecule having a cleavage site for a catalytic
nucleic acid molecule and a method for determining relative amounts of correctly capped RNA molecules and reverse-capped RNA molecules in a
population of RNA molecules, wherein the
population comprises correctly capped and / or reverse-capped RNA molecules that have a cleavage site for a catalytic
nucleic acid molecule. Moreover, the present invention provides uses of a catalytic nucleic
acid molecule.