The invention discloses a vertical semi-dovetail tenon node connection structure for a wood structure building beam column. In the wood structure building, the connection between a middle column and a wood beam or a side column and the wood beam adopts the vertical semi-dovetail tenon node connection structure, and knotting to each other is conducted through the tenon vertical semi-dovetail on the wood beam structure, so that the seismic horizontal bearing tensile strength of a frame is improved. Meanwhile, the vertical semi-dovetail tenon node has strong bending resistance ability. The vertical semi-dovetail tenon node is connected and applied to a wood structure farm house or a civil mixed structure, so that the seismic bearing ability of the wood structure farm house or the civil mixed structure is greatly improved; and the vertical semi-dovetail tenon node connection form makes a slight change on the basis of inheriting a traditional mortise and tenon node connection form, a nail and an iron are still not needed, the vertical semi-dovetail tenon node connection form is more suitable for drawing materials and constructing houses locally of remote mountainous areas, and the construction progress and construction quality are improved in a large degree; and meanwhile, cost of vertical semi-dovetail tenon node is low, and the cost of engineering structure can be reduced.