The invention discloses a safety venous indwelling needle with a needleless interface positive-pressure closing valve, comprising a puncture needle, a puncture needle seat and a handle, wherein, the puncture needle is formed into a puncture needle protection assembly by a puncture protection sleeve, a spring, a plunger latch and an escapement clamp; a venous indwelling needle is formed by an indwelling needle soft tube, a puncture needle guide tube, an indwelling needle seat and a sealing plug; a needless interface positive-pressure closing valve is formed by a shell, a piston, a seal ring, a spring, a connecting soft tube and a base; the positive-pressure closing valve and a liquid out plug separated structure do not involve the indwelling needle, are easy to operate, can reduce pain, and avoid the indwelling needle from taking off; the puncture needle protection sleeve can protect the puncture needle before and after the use of the indwelling needle, has the advantages of without added length, small and exquisite volume, good hand feel, accurate control, high puncture precision, and convenience for puncture operation and the like, can automatically retract to protect the puncture needle, can avoid acupuncture injury on medical workers, has positive pressure closing function, can prevent venous blood flowing back and overflowing, prevent blood from being polluted and avoid the blood from forming blocking in the indwelling needle, does not need heparin tube closing and other operation, has needless connection function, and can avoid needle injury, improve working efficiency and protect the safety of medical workers and patients.