Preparation method of glutathione-enriched yeast
A technology of glutathione and yeast, which is applied in the field of preparation of glutathione-rich yeast, can solve the problem of low glutathione content, achieve the effect of increasing added value, good improvement effect, and small loss of activity
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Example Embodiment
[0020] Embodiment one: fermentation culture in shake flask prepares yeast rich in glutathione
[0021] (1) Strain:
[0022] Saccharomyces cerevisiae( Saccharomyces cerevisiae ) CICC1532, preserved in Laboratory of Bioengineering 209, Hubei University of Technology.
[0023] (2) Culture medium:
[0024] Each 1L of the slant medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, 20g of agar, and the pH of the slant medium is 5.8-6.2;
[0025] Each 1L of the seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the slant medium is 5.8-6.2;
[0026] Each 1L of the shake flask level fermentation medium contains: glucose 25.95g, yeast powder 11.47g, ammonium sulfate 10.16g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.2g, the fermentation medium pH5.5-6.0;
[0027] (3) Seed expansion culture:
[0028] After the slanted seeds are activated for 12-16 hours, they are inserted into the Erlenmeyer flask loaded with the ...
Example Embodiment
[0046] Example 2 30L fermentor is used to prepare glutathione-rich yeast by fed-feed fermentation
[0047] (1) Strain
[0048] Saccharomyces cerevisiae( Saccharomyces cerevisiae ) CICC1532, preserved in Laboratory of Bioengineering 209, Hubei University of Technology.
[0049] (2) Culture medium
[0050] Each 1L of the slant medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, 20g of agar, and the pH of the slant medium is 5.8-6.2;
[0051] Each 1L of the seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the slant medium is 5.8-6.2;
[0052] Each 1L of the fermentation medium on the shake flask contains: 25.95g of glucose, 11.47g of yeast powder, 10.16g of ammonium sulfate, 2g of potassium dihydrogen phosphate, 0.2g of magnesium sulfate, and the pH of the fermentation medium is 5.5-6.0;
[0053] The fermentation medium on the level of each 1L of the fermenter contains: 9L of water, 100g of yeast powder, and the pH of th...
Example Embodiment
[0073] Embodiment three: on 60 cubic fermenters, adopt flow-feeding fermentation culture
[0074] (1) Strain
[0075] Saccharomyces cerevisiae( Saccharomyces cerevisiae ) CICC1532, preserved in Laboratory of Bioengineering 209, Hubei University of Technology.
[0076] (2) Culture medium
[0077] Each 1L of the slant medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, 20g of agar, and the pH of the slant medium is 5.8-6.2;
[0078] Each 1L of the primary seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the slant medium is 5.8-6.2;
[0079] Each 1 L of the secondary seed medium contains: 20 g of glucose, 20 g of peptone, 5 g of yeast extract, and the pH of the slant medium is 5.8-6.2;
[0080] Each 1L of the tertiary seed medium contains: 20g of glucose, 20g of peptone, 5g of yeast extract, and the pH of the slant medium is 5.8-6.2;
[0081] The fermentation medium on the level of each 1 L of the fermenter contains...
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