187results about How to "Little loss of activity" patented technology

The invention relates to a new production process for refining crude heparin sodium. The new process comprises the following steps of: firstly, preparing biological enzyme powder which is porcine trypsin hydrolase powder; then, mixing self-made content-known crude heparin sodium and a sodium chloride solution in a certain proportion to prepare a crude heparin sodium solution; removing protein by catalytically cracking, adjusting acid content and oxidizing; and carrying out the processes and biological technical methods of low-temperature centrifugal separation, ultrafiltration fraction, concentration, precipitation, dehydration, drying and the like to obtain a heparin sodium fine product. The invention has the advantages of short production period, low raw and auxiliary material energy consumption, high product recovery rate, good purity, high valence and good quality; when the recovery valence of the product is 150-160 u/mg, the recovery rate can be higher than 96 percent; and other indexes can reach the requirements of the national pharmacopeia standard.

The invention discloses pediococcus pentosaceus and application thereof. The Latin name of the pediococcus pentosaceus is Pediococcus pentosaceus LI05, and the pediococcus pentosaceus is preserved in the China General Microbiological Culture Collection Center and has a preservation number of CGMCC No.7049. The pediococcus pentosaceus disclosed by the invention has the morphological characteristics that the thallus is rod-shaped, dose not generate spores, does not have motility, and has masculine Gram staining. The whole-cell fatty acid of the pediococcus pentosaceus disclosed by the invention comprises the following main components in percentage by weight: about 1.45% of 14:0, about 3.26% of 16:1w7c/16:1w6c, about 31.25% of 16:0, about 6.31% of 18:1w9c, about 38.59% of 18:1w7c, about 1.37% of 18:0 and about 14.59% of un18.846/19:1w6c. The invention also discloses a complete sequence of 16S ribosome DNA (16SrDNA) of the pediococcus pentosaceus. The pediococcus pentosaceus and a preparation thereof disclosed by the invention can be used for adjusting the micro-ecological balance of intestinal tracts of human or animals, promoting digestive absorption and slowing down the happening and development of liver failure of experimental animals.

The invention belongs to the technical field of biological engineering, which relates to a method for separating and extracting boletic acid in situ in the process of utilizing rhizopus oryzae fermentation to prepare boletic acid. The invention aims at the special physicochemical properties of boletic acid, when rhizopus oryzae free cell is utilized to ferment, the invention adopts a hollow fiber ultrafilter membrane, concentration by nanofiltration and ion exchange adsorption coupling technique to separate and extract boletic acid. The operation condition of the method is mild, separation selectivity is strong, activity loss of somatic cell is small, fermentation efficiency of rhizopus oryzae is effectively improved, process flow of utilizing a biological method to synthesize boletic acid is reduced, and meanwhile, the invention utilizes a nanofiltration device to concentrate fermentation liquor in the process of reacting and separating, which greatly improves adsorption capacity of ion exchange resin to boletic acid, and material consumption and energy consumption are relatively lower, and waste and secondary pollutants which are produced are relative little. The reacting and separating process of the method are fast, complete, and operation stability is excellent, the method is a high-effective and novel mass transfer separation coupling strengthening technique, which has wider industrialized production prospect.

The present invention discloses a fermented feed-containing 5% laying hen premix and a laying hen full price daily diet prepared from the premix. The fermented feed consists of corn, tenebrio molitor meal, soybean meal, cottonseed meal, apple residues, chili meal, corn soaking liquid sprayed corn husk, wheat bran, microorganism liquid and water. The preparation steps of the fermented feed are as follows: raw material crushing and weighing, raw material primary mixing, strain activating, strain and raw material secondary mixing, solid anaerobic fermenting, and wet basic state fermentation product drying. The fermented feed-containing 5% laying hen premix comprises dry basic state fermentation feed and other materials. The daily diet consists of corn, soybean meal, stone powder, and the fermented feed-containing 5% laying hen premix. The advantages of the daily diet are that after the laying hens are fed by the daily diet, the laying hen egg production rate and other egg laying performances and egg product quality are significantly improved, egg production peak period is extended, immune function is enhanced, anti-stress ability is improved, and the daily diet can superimposedly play the roles of regulating the microecology of beneficial bacterium flora and regulating neurotransmitter in the central nervous system.

The invention discloses an immobilized laccase, which adopts magnetic mesoporous carbon as a vector, the laccase is immobilized on the magnetic mesoporous carbon by means of the physical adsorption effect, magnetic nanoparticles are embedded in the pore canals of the magnetic mesoporous carbon, the amount of the laccase adsorpted on the magnetic mesoporous carbon is more than 140mg/g, and the enzyme activity recovery rate of the immobilized laccase is 60 to 95 percent. The invention correspondingly discloses a preparation method for the immobilized laccase, which includes the following steps:the magnetic mesoporous carbon and laccase solution are first prepared, the concentration of the laccase solution is 0.3mg/ml to 1.0mg/ml, and the pH value of the laccase solution is 3.0 to 6.0; the magnetic mesoporous carbon is then added into the laccase solution, and the solution is oscillated under the temperature of 25 DEG C to 30 DEG C for more than three hours; and after subsequent processing, the immobilized laccase is obtained. Since the immobilized laccase disclosed by the invention is immobilized on the basis of the magnetic mesoporous carbon, the laccase has excellent enzymological properties; and the preparation method for the immobilized laccase is easy to operate, and the cost is low.

The invention discloses a preparation method for separating a biochemical medicament from small sheep intestine mucosas, in particular a preparation method for producing sodium heparin by using small sheep intestines. The preparation method for producing the sodium heparin by using the small sheep intestines is characterized by comprising the following steps of: preparation of intestine mucosas, enzymolysis, adsorption, elution, precipitation, desalting, dehydration and drying to obtain the sodium heparin. Therefore, the method has the advantages of high yield, low sodium heparin activity loss, stable quality and capability of solving the problem of stink in a sodium heparin workshop by adopting a workshop waste gas purification treatment device; 10,000 to 12,000 small sheep intestine mucosas are needed for producing 100 million units of sodium heparin by using the conventional process, and 6,000 to 8,000 small sheep intestine mucosas are needed for producing 100 million units of sodium heparin by using the preparation method of the invention; and the valence and activity of the sodium heparin are greatly improved so that the production cost is greatly reduced, and the profit is improved by over 40 percent.

A chitosan microsphere or microcapsule is prepared through proportionally mixing oil phase with surfactant, stirring, proportionally adding chitosan solution (water phase), stirring, proportionally adding ammonium sulfate solution, stirring, centrifugal separation of deposit, washing and drying.

The invention relates to a process method of oxidized graphene directional immobilization glucose oxidase. The process method comprises the following steps that 1) oxidized graphene is dissolved in distilled water, and oxidized graphene turbid liquid with the concentration being 1mg/mL is obtained through ultrasonic mixing; N-hydroxy sulfo-succinimide and 1-ethide-3-(3-dimethyl aminopropyl) carbodiimide are added into the turbid liquid, the washing and the separation are carried out after shaking, and esterified oxidized graphene is obtained; and 2) activated concanavalin A is added into the uniform esterified oxidized graphene, the centrifugation is carried out after reaction, and sediments are fully washed; and then, glucose oxidase solution is added, the full washing is carried out after reaction, and the directional immobilization glucose oxidase is obtained after the vacuum freeze-drying for 24 hours. The method for preparing the immobilized enzyme has the advantages that the immobilization efficiency is high, and the defects that the orientation of enzyme activity sites is inconsistent, or the enzyme activity sites are covered, and the like are avoided, so the activity of the immobilized enzyme is improved, the enzyme activity loss is low, and the enzyme activity can reach 150 to 195U/mg.

The invention relates to the technical field of preparation of microorganism carriers and in particular relates to a preparation method of a hydrogel embedded microorganism carrier. The preparation method comprises the following steps: extracting root nodule bacteria extract from soybean roots and mixing the root nodule bacteria extract with a lactobacillus alimentarius strain to prepare a mixed strain; inoculating active sludge with the mixed strain to obtain active sludge bacterium liquid; adding nutrient substances into the active sludge bacterium liquid and culturing to obtain sludge turbid liquid; mixing a polyurethane pre-polymer and the sludge turbid liquid and pouring a mixture into a mold; adding activated carbon powder, a catalyst, an initiator and the like and reacting to obtaina polyurethane embedded nitrifying bacterium gel block; finally, dicing the polyurethane embedded nitrifying bacterium gel block and mixing with self-made activated nano-powder to obtain a loading matrix; then immersing and carrying out film formation to obtain the hydrogel embedded microorganism carrier. According to the preparation method provided by the invention, nitrogen source pollutants insewage can be effectively degraded through re-nitrifying bacteria, and the hydrophilicity of the microorganism carrier is improved through hydrogel prepared from potassium persulfate and tetramethylethylenediamine; the biological resistance of root nodule bacteria is strong; the preparation method has a wide application prospect.

The present invention provides a hydrodesulfurization catalyst, which comprises a modified titania-alumina composite carrier and nickel phosphide loaded on the carrier, wherein the modified titania-alumina composite carrier comprises an alkaline earth metal component and a group VIB metal component. The catalyst of the present invention has low temperature hydrogenation activity and good stability when the catalyst is used for the C5-C9 distillate oil hydrodesulfurization process. The present invention further provides a preparation method of the catalyst and a C5-C9 distillate oil hydrodesulfurization method.

The invention provides a method for quickly preparing recombinant heat-resistant manganese superoxide dismutase and belongs to the technical field of bioseparation engineering. In the method, the metal-chelating chromatographic separation technology and the self-prepared ultra-macroporous metal-chelating medium are adopted, high-purity high temperature-resistant manganese superoxide dismutase can be quickly separated from recombinant Escherichia coli by four steps, namely centrifugation of fermentation liquor, cell breakage, centrifugation of breaking liquid and chromatography. The separation rate is over 5 times higher than the conventional chromatographic separation rate, the loss of SOD enzymatic activity is low, the recovery is high, the purity is over 85 percent, and the method makes mass production of low-cost high-purity SOD enzyme possible.

The invention provides a method for concentrating, stabilizing and separating liquid enzyme. The liquid enzyme comprises the following components by weight percent: 1.0-20.0% of macrocyclic molecular synthetic receptor, 0.1-50% of nonionic surfactant, 0.25-5% of enzyme stabilizer, 0.01-80% of biological enzyme, and the balance of polar solvent. The method comprises the following steps: mixing the components, and reacting for 1-24 hours under the conditions that the pH is 3-12 and the temperature is 0-80 DEG C; forming supramolecular microspheres containing the biological enzyme in the solution, wherein the particle size of the supramolecular microspheres is 10-1000nm. The selected materials are friendly to the environment, harmless, and easy to degrade. Above all, the method does not damage the activity of enzyme, the enzyme has high activity within an extended period, and the activity of the enzyme coated in the supramolecular microspheres is improved by 20% in comparison with the activity of common liquid enzyme. The activity loss of the biological enzyme is smaller than 10% four weeks after the liquid enzyme is applied to the formula of a textile emulsifier or a liquid detergent.

The invention belongs to the technical field of fuel cell catalyst carriers, and provides a composite catalyst carrier for a fuel cell, a preparation method thereof, and an application thereof in a fuel cell catalyst. The prepared Pt/TiC/TiON/SiON composite catalyst having a core-shell structure has a high activity, allows the Pt load capacity to be 0.1-0.15 mg/cm<2>, has a low cost, and also contains a small amount of Pd, a SiO2/TiO2 composite microsphere is doped with N to obtain a TiON/SiON composite material used as a shell structure, titanium carbide is used as a core, so the carrier canguarantee the chemical stability of the titanium carbide, guarantee high electron conductivity in an acidic medium, guarantee the activity and the catalysis efficiency of the catalyst and prolong theservice life of the catalyst active material Pt, has excellent performances and a large specific surface area, provides a reliable place for catalyst loading, has a strong anticorrosion performance, can effectively avoid poisoning and inactivation of the Pt catalyst, and has high application values.

The invention discloses a method for simply preparing an amorphous alloy CuB23 nano short tube and application thereof. The method comprises the following steps: (1) adding a copper acetate solution into a NH3.H2O solution with a concentration of 25 percent by weight to obtain a Cu(NH3)42+compound; (2) then adding a KBH4 aqueous solution and polyethylene glycol in the argon protective atmosphere at the temperature of 298K; (3) performing plasma reaction for 5-20 minutes to obtain a target product; (4) washing the product with deionized water for one time, then washing with anhydrous ethanol for three times; (5) storing the product in an ethanol solution for standby. In the Heck-coupling reaction, the CuB23 nano tube is an effective catalyst for substituting Pd and Ni complexes, is cheaper and does not need any ligand. Meanwhile, after being recycled for 10 times, the catalyst has small activity loss and has no crystal shape and structure change.

The invention provides a preparation method for an immobilization particle used for reducing activated sludge. The preparation method comprises the following steps: uniformly mixing fermentation broths of corresponding strains according to a weight ratio of Bacillus subtilis to Bacillus cereus to Bacillus megaterium to Bacillus licheniformis to Bacillus pumilus of 4-6: 4-6: 6-9: 5-7: 4; preparing bacterial powder from the above mentioned mixed bacterial liquid through spray drying; and preparing the immobilization particle by using a PVA-saturated boric acid method. By using an immobilization method, high-efficiency sludge degrading bacterial strains are embedded, so the concentration of the sludge degrading bacterial strains participating in a reaction can be greatly increased, the bacterial strains are vulnerable to loss and dying, have longer action time and obviously improved antitoxin capability and tolerance, solid-liquid separation can be easily realized, and secondary pollution is small.

The invention relates to the technical field of waste catalyst recycling, in particular to an ultrasonic pickling regeneration method for a waste flue gas denitration catalyst. The ultrasonic pickling regeneration method comprises the following steps: (1) dust collection: blowing and cleaning dust on the surface of an inactivated catalyst sample; (2) washing: after the sample is subjected to the dust collection, soaking the sample into ionized water for ultrasonic washing through an ultrasonic generator; (3) acid pickling: after the sample is washed, putting the sample into a mixed acid solution consisting of fatty alcohol-polyoxyethylene ether, polyethylene glycol fatty acid ester and sulfuric acid, and performing ultrasonic acid pickling through the ultrasonic generator; (4) drying: putting the sample subjected to the acid pickling into a vacuum drying box for drying to obtain a regenerated catalyst. Compared with the conventional waste flue gas denitration catalyst regeneration technology, the ultrasonic pickling regeneration method disclosed by the invention has the advantages that the problem of inactivation of the catalyst due to blockage and poisoning can be solved at one time; furthermore, the cleaning step is eliminated, the loss of the activity of the catalyst is reduced, and the water pollution is alleviated.

A method for extracting red jujube polyphenol comprises the following steps: 1) crushing red jujube peels and/or jujube pi to be put into a closed container, adding a 2-4 mol/L NaOH mixed solution comprising 1% of Vc in mass ratio and 10 mmol/L EDTA according to the solid-to-liquid ratio of 1:(10-15) (w/v) to form a reaction liquid, filling nitrogen gas in the container, and then sealing; 2) transferring the sealed container into a shaking table at the temperature of 25 DEG C to shake; 3) unsealing the sealed container, quickly adjusting the pH value of the reaction liquid in the container to be 2.0 through 6 mol/L HCl; conducting repeated extraction through ethyl acetate with the same volume as the reaction liquid till ethyl acetate phase is colorless, and collecting and combining the ethyl acetate phase; 5) evaporating under the condition of decompressing and condensing at the temperature of not larger than 40 DEG C to remove ethyl acetate in the ethyl acetate phase so as to obtain a red jujube polyphenol extractive. The method for extracting the red jujube polyphenol is simple in process and high in extraction ratio.