Preservation method of anaerobic ammonium oxidation strain

Abstract

According to the embodiment, the invention provides a preservation method of an anaerobic ammonium oxidation strain. The preservation method comprises the following steps: cultivating the anaerobic ammonium oxidation until it is stable in operation; preparing simulation wastewater; preparing preservation bacterium liquid and transferring the preservation bacterium liquid to a preservation device; air-stripping the preservation bacterium liquid in the preservation device until dissolved oxygen (DO) in the bacterium liquid is lower than 0.1mg / l, regulating pH value of the bacterium liquid to 7.5-7.6, then inflating protective gas to the top of the preservation device and sealing the preservation device; and conducting medium-temperature preservation. According to the method, on the basis of an anaerobic ammonium oxidation bacterium denitrification mechanism, a gaseous phase is transformed into NO gas as the protective gas at a certain concentration in a preserving course, so that the utilization of other denitrifying bacteria on a matrix in the preserving course is effectively inhibited and the matrix is continuously provided to anaerobic ammonium oxidation bacteria; the strain is high in survival rate, low in activity loss and more complete in biological performance preservation; and the method is simple and is easy to implement.

Description

technical field [0001] The invention relates to the technical field of strain preservation, in particular to a method for preserving anammox strains. Background technique [0002] Anaerobic ammonia oxidation is a new type of biological denitrification mode, which can use nitrite as the electron acceptor and ammonia nitrogen as the electron donor to convert the two into nitrogen under anaerobic conditions, so as to realize the denitrification of two kinds of nitrogen pollutants. removed at the same time. Since ammonia nitrogen and nitrite nitrogen can be removed at the same time, there is no need to add organic carbon sources, and it can improve the neutralization of acid production in nitrification reaction and alkali production in denitrification reaction. Its operating cost is nearly 40% lower than that of traditional biological denitrification processes. Therefore, compared with other biological denitrification modes, ANAMMOX has obvious advantages. However, anammox bac...

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Problems solved by technology

[0012] Existing preservation methods mainly focus on the selection of liquid-phase preservation conditions such as concentrated preservation temperature and the preservation process, such as substrates and protective agents. Although gas-phase conditions are of great significance to the life activities of anaerobic ammonium bacteria, the current method does not involve Optimization of Gas Phase Conditions During Preservation

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