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Immobilized laccase and its preparation method

A technology for immobilizing laccase and laccase, which is applied in the direction of immobilizing on or in the inorganic carrier, can solve the problems of cumbersome preparation process and difficult separation, and achieve easy separation operation, small activity loss and good biocompatibility Effect

Inactive Publication Date: 2011-12-21
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been reported in the literature that immobilizing laccase with magnetic mesoporous silicon with a large pore size has a higher activity recovery rate of the immobilized enzyme, and improves the pH stability and thermal stability of the laccase (Yufang Zhu et al, Chemistry of Materials, 2007 , 6408-6413), but this kind of mesoporous material has the disadvantages of cumbersome preparation process and difficult separation.

Method used

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  • Immobilized laccase and its preparation method
  • Immobilized laccase and its preparation method
  • Immobilized laccase and its preparation method

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Embodiment 1

[0042] An immobilized laccase of the present invention, the immobilized laccase comprises magnetic mesoporous carbon and laccase with a mass ratio of 1:2.5, the immobilized laccase uses magnetic mesoporous carbon as a carrier, and the laccase is fixed by physical adsorption On the carrier magnetic mesoporous carbon, magnetic nanoparticles are embedded in the pores of the magnetic mesoporous carbon; the magnetic mesoporous carbon in this embodiment has an ordered bimodal mesoporous structure, and the two peaks of the pore size distribution appear at 4nm respectively. and 18nm.

[0043] A preparation method of the above-mentioned immobilized laccase, comprising the following steps:

[0044] (1) Preparation of magnetic mesoporous carbon: (a) first place 4.0 g of block copolymer Pluronic P123 (manufactured by Sigma, with a molecular weight of 5800) in 160 ml of HCl with a concentration of 1.54 M, and stir in a water bath at 35 ° C until Dissolved, then added 8.5g tetraethyl ortho...

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Abstract

The invention discloses an immobilized laccase, which adopts magnetic mesoporous carbon as a vector, the laccase is immobilized on the magnetic mesoporous carbon by means of the physical adsorption effect, magnetic nanoparticles are embedded in the pore canals of the magnetic mesoporous carbon, the amount of the laccase adsorpted on the magnetic mesoporous carbon is more than 140mg / g, and the enzyme activity recovery rate of the immobilized laccase is 60 to 95 percent. The invention correspondingly discloses a preparation method for the immobilized laccase, which includes the following steps:the magnetic mesoporous carbon and laccase solution are first prepared, the concentration of the laccase solution is 0.3mg / ml to 1.0mg / ml, and the pH value of the laccase solution is 3.0 to 6.0; the magnetic mesoporous carbon is then added into the laccase solution, and the solution is oscillated under the temperature of 25 DEG C to 30 DEG C for more than three hours; and after subsequent processing, the immobilized laccase is obtained. Since the immobilized laccase disclosed by the invention is immobilized on the basis of the magnetic mesoporous carbon, the laccase has excellent enzymological properties; and the preparation method for the immobilized laccase is easy to operate, and the cost is low.

Description

technical field [0001] The invention relates to the field of enzyme immobilization, in particular to an immobilized laccase and a preparation method thereof. Background technique [0002] Laccase (laccase, E.C.1.10.3.2) is a copper-containing polyphenol oxidase widely found in fungi, bacteria and plants. Laccase has a wide range of substrate specificity and strong stability, and can catalyze many phenols and aromatic amines, such as phenol, p-chlorophenol, aniline, and polyphenols and polyamines. , biofuel cells, biotransformation and wastewater treatment and other fields have a wide range of applications. However, since laccase is easily soluble in water, the free laccase in water cannot be recovered or stored for a long time, and the stability of laccase is not easy to maintain under high temperature or other extreme environments, thus limiting the practical application of laccase. Immobilization technology not only maintains the specific and efficient catalytic activity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C01B31/02
Inventor 汤琳刘媛媛曾光明庞娅雷晓霞伍梦诗李贞危臻
Owner HUNAN UNIV
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