Immobilized laccase and its preparation method
A technology for immobilizing laccase and laccase, which is applied in the direction of immobilizing on or in the inorganic carrier, can solve the problems of cumbersome preparation process and difficult separation, and achieve easy separation operation, small activity loss and good biocompatibility Effect
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[0042] An immobilized laccase of the present invention, the immobilized laccase comprises magnetic mesoporous carbon and laccase with a mass ratio of 1:2.5, the immobilized laccase uses magnetic mesoporous carbon as a carrier, and the laccase is fixed by physical adsorption On the carrier magnetic mesoporous carbon, magnetic nanoparticles are embedded in the pores of the magnetic mesoporous carbon; the magnetic mesoporous carbon in this embodiment has an ordered bimodal mesoporous structure, and the two peaks of the pore size distribution appear at 4nm respectively. and 18nm.
[0043] A preparation method of the above-mentioned immobilized laccase, comprising the following steps:
[0044] (1) Preparation of magnetic mesoporous carbon: (a) first place 4.0 g of block copolymer Pluronic P123 (manufactured by Sigma, with a molecular weight of 5800) in 160 ml of HCl with a concentration of 1.54 M, and stir in a water bath at 35 ° C until Dissolved, then added 8.5g tetraethyl ortho...
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