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The invention specifically discloses a myofibrillar protein-sunflower seed oil-high M type alginic acid ternary composite probiotic microcapsule and a preparation method thereof, and belongs to the technical field of food. Myofibrillar protein and sodium alginate are used as inner wall materials, gelatin and sunflower seed oil are used as outer wall materials, and lactobacillus acidophilus is embedded in a double-layer mode through electrostatic interaction. The specific preparation method comprises the following steps: mixing a bacterial suspension with the composite wall material, dropwise adding the mixture into pure water, and carrying out ultrasonic curing, dewatering and vacuum freeze drying to obtain the microcapsule. The microcapsule prepared by the invention has a good embedding rate, can play a role in protection and slow release in a simulated digestion process, improves the thermal stability and storage stability of probiotics, and has a wide application prospect.