Method for improving cellulase use efficiency, and apparatus thereof

A technology of cellulase and cellulose, which is applied in the direction of biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of product inhibition, reduce the efficiency of cellulase use, and reduce the efficiency of enzymatic hydrolysis, so as to increase the contact area and the effect of time

Inactive Publication Date: 2015-09-16
镇江博睿兴邦生物科技有限公司
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Problems solved by technology

[0006] 3. The usual hydrolysis process is intermittent. With the gradual degradation of cellulose, its content is getting less and less, so the probability of contact between cellulase and cellulose is gradually decreasing, and at this time there are still some enzymes in the system It has high activity, which is also one of the

Method used

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  • Method for improving cellulase use efficiency, and apparatus thereof

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Embodiment 1

[0031] (1) Processing of bagasse

[0032] 1. Take by weighing 10kg of dry bagasse, use 30L mass concentration of 5% dilute sulfuric acid to process the cellulose raw material to extract the hemicellulose in the cellulose raw material;

[0033] ② Filter out the solid from the solution treated in step (1) ①, wash the solid with water until the pH is 6.5-7.0; collect the filtrate at the same time, and the filtrate is dilute sulfuric acid liquid containing hemicellulose;

[0034] ③ Soak the solid obtained through step (1) ② with an aqueous sodium hydroxide solution with a mass concentration of 15% at 120 degrees for 5 hours to remove lignin, filter out the processed bagasse, and oxidize The mass ratio of sodium aqueous solution to solid is 10:1;

[0035] ④ Dilute sulfuric acid liquid containing hemicellulose Continue to process the untreated cellulose in step (1) ①, and then go through step (1) ② to obtain dilute sulfuric acid liquid containing hemicellulose, which can be used re...

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Abstract

The invention relates to a method for improving the cellulase use efficiency, and an apparatus thereof. The method comprises the following steps: 1, extracting hemicellulose by using diluted sulfuric acid, immersing in an alkali to remove lignin, and processing a cellulose raw material; and 2, hydrolyzing the processed cellulose raw material by cellulase in an enzymatic hydrolysis reactor. The method and the apparatus avoid adsorption of lignin to cellulase and increase the hemicellulose utilization rate, and the enzymatic hydrolysis reactor is not provided with a stirring device to solve a problem of enzyme inactivation caused by excessive stirring of traditional enzymatic hydrolysis reactors; a traditional intermittence technology is changed to a continuous technology, so the product inhibition problem in the process is solved; and the cellulase use amount in the method and the apparatus in the invention is obviously lower than that of traditional technologies.

Description

technical field [0001] This patent relates to a method and a device for improving use efficiency and reducing usage amount when cellulase is used to enzymatically hydrolyze cellulose. Background technique [0002] Cellulose hydrolysis technology currently mainly includes dilute sulfuric acid hydrolysis and cellulase hydrolysis, in which the efficiency of enzymatic hydrolysis and the selectivity and purity of the final product are higher. However, the high cost of cellulase for enzymatic hydrolysis is currently difficult to overcome difficulty. [0003] At the current technical level of cellulase, the reasons for the high cost of cellulase use are as follows: [0004] 1. It is related to the structure of the plant itself. The cellulose of some plants has high crystallinity and dense structure. Although it has been pretreated by various means, it is still difficult to destroy the structure so that the cellulase can contact the cellulose. [0005] 2. After pretreatment, a la...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12P19/14
CPCC12P19/14
Inventor 吕涛
Owner 镇江博睿兴邦生物科技有限公司
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