Method for improving cellulase use efficiency, and apparatus thereof

Abstract

The invention relates to a method for improving the cellulase use efficiency, and an apparatus thereof. The method comprises the following steps: 1, extracting hemicellulose by using diluted sulfuric acid, immersing in an alkali to remove lignin, and processing a cellulose raw material; and 2, hydrolyzing the processed cellulose raw material by cellulase in an enzymatic hydrolysis reactor. The method and the apparatus avoid adsorption of lignin to cellulase and increase the hemicellulose utilization rate, and the enzymatic hydrolysis reactor is not provided with a stirring device to solve a problem of enzyme inactivation caused by excessive stirring of traditional enzymatic hydrolysis reactors; a traditional intermittence technology is changed to a continuous technology, so the product inhibition problem in the process is solved; and the cellulase use amount in the method and the apparatus in the invention is obviously lower than that of traditional technologies.

Description

technical field [0001] This patent relates to a method and a device for improving use efficiency and reducing usage amount when cellulase is used to enzymatically hydrolyze cellulose. Background technique [0002] Cellulose hydrolysis technology currently mainly includes dilute sulfuric acid hydrolysis and cellulase hydrolysis, in which the efficiency of enzymatic hydrolysis and the selectivity and purity of the final product are higher. However, the high cost of cellulase for enzymatic hydrolysis is currently difficult to overcome difficulty. [0003] At the current technical level of cellulase, the reasons for the high cost of cellulase use are as follows: [0004] 1. It is related to the structure of the plant itself. The cellulose of some plants has high crystallinity and dense structure. Although it has been pretreated by various means, it is still difficult to destroy the structure so that the cellulase can contact the cellulose. [0005] 2. After pretreatment, a la...

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Problems solved by technology

[0006] 3. The usual hydrolysis process is intermittent. With the gradual degradation of cellulose, its content is getting less and less, so the probability of contact between cellulase and cellulose is gradually decreasing, and at this time there are still some enzymes in the system It has high activity, which is also one of the factors that reduce the efficiency of cellulase; during the intermittent enzymatic hydrolysis reaction, product inhibition will occur, which can also reduce the enzymatic hydrolysis efficiency

However, the research found that cellulase is very sensitive to the shear of stirring, and excessive stirring will cause enzyme inactivation

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