A kind of blueberry anthocyanin separation and purification method

A technology for the separation and purification of blueberry anthocyanins, applied in the field of separation and purification of blueberry anthocyanins, can solve the problems of easy deactivation, low extraction rate and low purity of blueberry anthocyanins, and achieve the effect of increased purity

Active Publication Date: 2022-06-17
HEILONGJIANG LVZHIDU BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems of low extraction rate, low purity and easy inactivation of blueberry anthocyanins in the existing extraction methods, the present invention provides a method for separating and purifying blueberry anthocyanins, which comprises the following steps:

Method used

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  • A kind of blueberry anthocyanin separation and purification method
  • A kind of blueberry anthocyanin separation and purification method
  • A kind of blueberry anthocyanin separation and purification method

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1. Method for separation and purification of blueberry anthocyanins.

[0030] 1) Take 1000 g of blueberry fruit, crush it with a high-speed crusher, and crush it with a particle size of 100 mesh to obtain pulp, then add cellulase with an activity unit of 1000 U / g, and the addition amount is 0.1 g / kg pulp, at pH 4.5 , the temperature is 45° C., the rotational speed of the stirring paddle is 20 rpm, and the enzymatic hydrolysis is carried out by stirring for 40 minutes to obtain a cellulase enzymatic hydrolysis solution.

[0031] 2) Then add ethanol with a volume fraction of 60% at a ratio of 1 g:4 mL of material to liquid, and put it into a horizontal grinding bead extraction equipment for grinding and extraction to obtain an extract. The diameter of the grinding beads is 2.0 mm, the loading of the grinding beads is 30% of the volume of the grinding kettle, the stirring revolution is 20 times / min, and the extraction time is 40 min. During the extraction process,...

Embodiment 2

[0034] Example 2. Blueberry anthocyanin separation and purification method.

[0035] 1) Take 1000g of blueberry fruit, crush it with a high-speed crusher, and crush the particle size of 100 mesh to obtain pulp, then add cellulase with an activity unit of 1000U / g, and the addition amount is 0.2g / kg pulp, at pH: 5 -6, the temperature is 50°C, the rotational speed of the stirring paddle is 25 revolutions / min, and the enzymatic hydrolysis is carried out by stirring for 50 minutes to obtain a cellulase enzymatic hydrolysis solution.

[0036] 2) Then add ethanol with a volume fraction of 60% at a material-to-liquid ratio of 1 g:6 mL, and put it into a horizontal grinding bead extraction kettle for grinding and extraction to obtain an extract. The diameter of the grinding beads is 1.0 mm, and the loading amount of the grinding beads is 50% of the volume of the grinding kettle. The stirring revolution was 30 times / min. The extraction time was 30 min. During the extraction process, ...

Embodiment 3

[0039] Example 3. Method for separation and purification of blueberry anthocyanins.

[0040] 1) Take 1000g of blueberry fruit, crush it with a high-speed crusher, and crush the particle size of 100 mesh to obtain pulp, and then add cellulase with an activity unit of 1000U / g, and the addition amount is 0.2g / kg pulp, at pH: 6.5 , the temperature is 55 ℃, the rotation speed of the stirring paddle is 20 rpm, and the stirring is carried out for 50 minutes to carry out enzymatic hydrolysis to obtain a cellulase enzymatic hydrolysis solution.

[0041] 2) Then add ethanol with a volume fraction of 60% at a material-to-liquid ratio of 1 g:5 mL, and put it into a horizontal grinding bead extraction kettle for grinding and extraction to obtain an extract. The diameter of the grinding beads is 3.0 mm, the loading of the grinding beads is 50% of the volume of the grinding kettle, the stirring revolution is 30 times / min, and the extraction time is 45 min. During the extraction process, coo...

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Abstract

The invention discloses a method for separating and purifying blueberry anthocyanins, which belongs to the technical field of plant active ingredient extraction. The present invention aims at the problems of low extraction rate, low purity and easy inactivation of blueberry anthocyanins in the existing extraction method. The blueberry is crushed, ground and beaten, and then enzymatically hydrolyzed by cellulase, low-temperature grinding and extracted, filtered, solvent recovered, Glucosidase enzymatic hydrolysis, coarse filtration, membrane filtration, chromatography, concentration, drying and other processes for separation and purification to obtain high-purity anthocyanins. The invention is applicable to the extraction and production of blueberry anthocyanins.

Description

technical field [0001] The invention relates to the technical field of plant active ingredient extraction, in particular to a method for separating and purifying blueberry anthocyanins. Background technique [0002] Blueberry anthocyanin is a functional food coloring bound to blueberry peel cellulose. It has important biological activity, is easy to oxidize, has heat sensitivity, and has extremely poor stability; it connects a large number of sugar chains through glycosidic bonds. The connection directly affects the color value and purity of anthocyanins. The existing extraction method cannot fully release anthocyanin from the blueberry peel, cannot guarantee the processing stability of anthocyanin during processing, and is difficult to obtain high extraction rate, biological activity and purity. SUMMARY OF THE INVENTION [0003] In view of the problems of low extraction rate, low purity and easy inactivation of blueberry anthocyanin in the existing extraction method, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62C09B61/00C09B67/54
CPCC07D311/62C09B61/00C09B67/0096
Inventor 王振宇刘勇刚景秋菊刘迪迪
Owner HEILONGJIANG LVZHIDU BIOTECH DEV
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