Method for separating and extracting fumaric acid

A technology of fumaric acid and reactor, which is applied in the field of bioengineering, can solve the problems that the adsorption capacity of resin has not been effectively utilized, it is difficult to realize industrial application, and the increase of energy consumption and material consumption has achieved wide industrial production prospects and operation. Good stability, low material consumption and energy consumption

Inactive Publication Date: 2008-08-06
NANJING UNIV OF TECH
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Problems solved by technology

However, under the fermentation conditions of this process, the concentration of fumaric acid in the fermentation liquid is low, and the adsorption capacity of the resin has not been effectively utilized, so that the amount of resin used in the fermentation process is relatively increased, energy consumption and material consumption are increased, and it is difficult to realize industrial application.

Method used

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  • Method for separating and extracting fumaric acid

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Embodiment 1

[0052] In conjunction with shown in Fig. 1, adopt the Rhizopus species Rhizopus Oryzae (Rhizopus Oryzae) ME-F11 (China Center for Type Culture Collection, preservation number is CCTCC NO: M 207066) The steps of in-situ separation and extraction of fumaric acid are as follows:

[0053] (1) Preparation of spore suspension: Inoculate Rhizopus Oryzae (Rhizopus Oryzae) ME-F11 on solid medium (yeast extract juice 5.0g / L, malt extract juice 5.0g / L, peptone 5.0g / L, glycerin 20g / L L, agar 20g / L) was cultured at 35°C for 6 days. After the spores matured, the spores were washed out with sterile water, placed in a sterilized Erlenmeyer flask filled with glass beads, fully shaken at 35°C and 120rpm for 50min, Evenly disperse the spores and filter them with sterilized absorbent cotton to obtain a spore suspension with a concentration of spores controlled at 10 6 pcs / ml spare;

[0054] (2) Seed culture: seed culture solution (glucose 40g / L, urea 3g / L, KH 2 PO 4 0.5g / L, MgSO 4 0.5g / L, F...

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Abstract

The invention belongs to the technical field of biological engineering, which relates to a method for separating and extracting boletic acid in situ in the process of utilizing rhizopus oryzae fermentation to prepare boletic acid. The invention aims at the special physicochemical properties of boletic acid, when rhizopus oryzae free cell is utilized to ferment, the invention adopts a hollow fiber ultrafilter membrane, concentration by nanofiltration and ion exchange adsorption coupling technique to separate and extract boletic acid. The operation condition of the method is mild, separation selectivity is strong, activity loss of somatic cell is small, fermentation efficiency of rhizopus oryzae is effectively improved, process flow of utilizing a biological method to synthesize boletic acid is reduced, and meanwhile, the invention utilizes a nanofiltration device to concentrate fermentation liquor in the process of reacting and separating, which greatly improves adsorption capacity of ion exchange resin to boletic acid, and material consumption and energy consumption are relatively lower, and waste and secondary pollutants which are produced are relative little. The reacting and separating process of the method are fast, complete, and operation stability is excellent, the method is a high-effective and novel mass transfer separation coupling strengthening technique, which has wider industrialized production prospect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for separating and extracting organic acids in the fermentation industry. Background technique [0002] Fumaric acid (fumaric acid) is an important bulk chemical, which can be widely used in food, chemical industry, medicine, coating, resin and other fields. At the same time, fumaric acid is also an important four-carbon platform compound, which can be Transformation, lipidation, hydrogenation and other processes to produce aspartic acid, malic acid, succinic acid, maleic acid, 1,4-butanediol and other four-carbon compounds. [0003] The production methods of fumaric acid include chemical synthesis and biological fermentation. The current main technology for fumaric acid production is chemical synthesis. However, due to the limitations of the technological level and people's understanding at that time, the biosynthesis of fumaric acid has always been ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C07C57/15C07C51/42C12R1/845
Inventor 黄和付永前张昆徐晴李霜
Owner NANJING UNIV OF TECH
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