34results about How to "Increased spore content" patented technology

A process for preparing the biologic agricultural chemical from trichoderma includes such steps as reproductive culture of trichoderma, fermenting in liquid, inoculating it in the culture medium prepared from cane dregs, wheat bran and rice husk for solid fermenting, culturing at 25-28 deg.C under 60-80% of relative humidity for 4-5 days, culturing at low temp (lower than 12 deg.C) for 2-3 days, drying at 40-50 deg.C for 12-24 hr, adding promoter prepared from diatomite, wheat bran and sulfur, mixing, pulverizing and sieving.

The invention discloses gene recombination bacillus amyloliquefaciens and a microbial inoculum preparation method. A bacillus amyloliquefaciens FZB42 strain is taken as a host bacterium to construct the gene recombination bacillus amyloliquefaciens XLV09-1 with transparent vitreoscilla hemoglobin which is regulated and controlled by a P43 strong promoter by contrasting a gene recombination vector. The produced spore number of microbial inoculum prepared by fermenting the bacillus amyloliquefaciens can reach more than 100 hundred millions per milliliter, the overall yield of antibacterial lipopeptid reaches more than 1 gram per liter and prevention effect on various fungal plant diseases is improved by over 30 percent. The microbial inoculum is a green microecological preparation, of no toxic residue, safe to human and livestock, and favorable for protecting the ecological environment.

The invention relates to the technical field of preparation of microbes and specifically relates to a method for efficiently improving the germination rate of Bacillus subtilis. According to the method provided by the invention, glycerine is added in a Bacillus subtilis fermentation broth as a fermentation strain, an inorganic salt solution capable of improving the spore germination rate is added when the fermentation strain is cultured for 15-18 hours, and the inorganic salt solution contains the following components in percentage by weight: 0.12-0.125% of MgSO4.7H2O, 0.01-0.015% of MnSO4.H2O, 0.05-0.1% of CaCl2, 0.005-0.008% of FeSO4.7H2O, 0.015-0.02% of KI and 0.035-0.04% of KH2PO4. The fermentation of the strain obtained by the method can achieve 5.8*10<11> CFU/ml, the number of spores is 5.49*10<11> CUF/ml, and the spore germination rate is 94.7%.

The invention discloses a Paenibacillus polymyxa GY40 bacterial strain. The preservation number is CGMCC NO.14481. The invention also discloses a spore production fermentation culture method of the Paenibacillus polymyxa GY40 bacterial strain, and a method used for preparing bacterial powder and a wettable powder from the Paenibacillus polymyxa GY40 bacterial strain. Compared with terrestrial microorganisms, the Paenibacillus polymyxa GY40 bacterial strain possesses following advantages: salt resistance is excellent, growth temperature is low, and antibacterial effect is excellent. The spore production fermentation culture method is simple in formula; the main raw materials are agricultural product leftovers; the cost is low; the raw materials are widely available; bacterial strain growthspeed is high; fermentation temperature is low; fermentation time is short; fermentation conditions are convenient to control; fermentation spore yield is high; raw material cost is reduced; fermentation energy consumption is reduced; fermentation production efficiency is increased; the wettable powder possesses excellent preventing and treating effect on wheat scab and fusarium wilt of cucumber,and can be used in plantation of wheat and cucumber.

The invention provides a culture medium of bacillus amyloliquefaciens and application, relating to the field of microorganisms. The fermentation medium contains 5-10g/L of glucose, 10-30g/L of molasses, 5-10g/L of silkworm chrysalis meal, 5-20g/L of corn steep liquor, 10-20g/L of sodium chloride, 1-10g/L of calcium carbonate, 0.1-1g/L of magnesium sulfate, 0.01-0.1g/L of manganese sulfate and 0.02-0.08g/L of ferrous chloride, and the pH value is 7.0-7.5. The bacillus amyloliquefaciens K-8 is cultured by the culture medium, and the culture conditions are controlled as follows: from the 0th-8th hour, the gas-liquid ratio is (0.4-0.6):1, and the temperature is 25-28 DEG C; from the 8th-24th hour, the gas-liquid ratio is (0.9-1.1):1, and the temperature is 28-30 DEG C; after the 24th hour, the gas-liquid ratio is (0.7-0.9):1, and the temperature is 30-32 DEG C. By adopting the culture medium and culture method provided by the invention, mass propagation of the bacillus amyloliquefaciens K-8 in relatively short time is facilitated so that a great quantity of spores are formed in relatively short time.

The invention discloses a preparation method and an application of a coniothyrium minitans ZS-1SB fungicide. The preparation method comprises the following steps of: (1) first, performing PDA (Potato Dextrose Agar) slant culture for the coniothyrium minitans ZS-1SB fungicide; washing the slant with sterile water, and adjusting a conidium suspension liquid to 106 spores/ml; (2) oscillating the conidium suspension liquid of coniothyrium minitans ZS-1SB prepared by the step 1 to obtain a coniothyrium minitans ZB-1SB bacterial liquid; and (3) uniformly mixing oatmeal and husks, adding water till the water content reaches 40%, filling by an edible fungus bagging machine, and steam sterilizing at normal pressure; after cooling, inoculating the coniothyrium minitans ZB-1SB bacterial liquid, and culturing in a certain condition by a 30ml/culture bottle. The yield of coniothyrium minitans prepared by the preparation method disclosed by the invention can reach 3.2*10<10> spore/g dry compost. Under field culture condition of an antibiological inoculant, soil broadcast fertilization and overground part in blossom period realizes the preventing effect of 57% to sclerotinia rot of colza. The fungicide can be applied to large-scale disease prevention and treatment.

The invention discloses an ocean-originated bacillus amyloliquefaciens GY30 strain. The preservation number of the ocean-originated bacillus amyloliquefaciens GY30 strain is CGMCC NO. 14480. The invention also discloses a spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain and a method for preparing bacterium power through the bacillus amyloliquefaciens GY30 strain. The bacillus amyloliquefaciens GY30 strain has the advantages of being high in salt tolerance, low in growth temperature, good in antibacterial effects, wide in antibacterial range and easy to culture, which do not exist in terrigenous microorganisms. The spore production fermentation medium composition and the culture method of the bacillus amyloliquefaciens GY30strain are simple, applied raw materials are mainly agricultural product leftovers, thereby being low in price, extensive in resources, high in strain growth speed, low in fermentation temperature, short in fermentation time, easy to control in fermentation conditions, high in spore fermentation yield and capable of reducing raw material costs and fermentation energy consumption and improving thefermentation production efficiency; bacterium powder prepared through the spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain is high ineffects on processing microorganism fertilizers or rice sheath blight disease preventing pesticides.

The invention provides a fermentation production method for hypocrea virens. A hypocrea virens strain is activated and cultured in a primary seed liquid medium and a secondary liquid medium for expanding reproduction, strong thalli and a certain number of spores are obtained, a solid medium formed by a compounded carbon source and nitrogen source is inoculated with secondary seed liquid, the compounded carbon source and nitrogen source are put in fermentation production, and meanwhile most proper non-bio impact factors are provided; rapid growth and spore production of hypocrea virens are promoted, and the content of the spores generated after solid fermentation is 100,000,000,000 per g.

The invention provides a culture medium used for producing metarhizium anisopliae spore powder and a production method of the high-content spore powder of metarhizium anisopliae. The culture medium comprises a liquid seed culture medium and a solid culture medium which are used for culturing the metarhizium anisopliae, wherein the liquid seed culture medium is composed of the following componentsin percentage by weight: 6% of yam powder, 4% of cockroach powder, 3% of glucose, 0.1% of K2HPO4, 0.05% of MgSO4 and the balance water, and a pH value is 6; and the solid culture medium is composed ofthe following components in percentage by weight: 10-12% of yam herbaceous climber powder, 30-40% of cockroach powder, 20-30% of yam powder, 2-3% of glucose and the balance water, and a pH value is 6. The production method comprises the following steps: rejuvenating the metarhizium anisopliae; conducting liquid seed culture to obtain metarhizium anisopliae mother liquor; and conducting secondaryculture by means of the solid culture medium, and collecting spores. According to the provided production method, no grains are used, a spore yield is high, pollution is not liable to be caused in a fermentation process, and conidia and a substrate are easy to separate.

The invention provides a preparation method of Streptomyces jingyangensis wettable powder. The method comprises the following steps: fermenting a carrier kaolin, Rhodopseudomonas palustris, and nutrients corn starch and bean pulp powder to obtain high-concentration Streptomyces jingyangensis powder; and mixing 90-95 parts of Streptomyces jingyangensis fermented powder, 1-4 parts of wetting agent sodium dodecyl benzene sulfonate, 0.2-1 part of dispersing agent tea saponin, 0.5-2 parts of ultraviolet protective agent ascorbic acid, 0.2-1 part of beta-dextrin, 1-4 parts of drying protective agent maltose and 0.2-1 part of mannitol to obtain the stable Streptomyces jingyangensis wettable powder with the bacterium content of more than 10 billion CFU/g.

The invention provides a fermentation production method of hypocrea virens. Strains of the hypocrea virens are activated and are cultured in a first-stage seed liquid culture medium and a second-stage liquid culture medium so as to expand reproduction, so as to obtain strong thallus and spores, and second-stage seed liquid is inoculated to a third-stage liquid culture medium formed by a composite carbon source and a nitrogen source, so that the hypocrea virens quickly grows and generates spores. The third-stage liquid culture medium is prepared from the following components according to mass percent: 2.00 to 3.00% of glucose, 0.50 to 1.50% of corn flour, 0.03 to 0.10% of ammonium sulfate, 0.03 to 0.10% of dipotassium phosphate, 0.07 to 0.20% of calcium chloride and the balance of water. The viable count in obtained fermentation liquid is 1 to 5 billion CFU/ml, and the yield of bacterial powder is 10% to 15% after spray drying. By adopting the technical scheme, hypocrea virens powder with high spore content can be obtained at last.

The invention discloses paecilomyces variotii powder as well as a preparation method and an application thereof. The powder is prepared by fermenting paecilomyces variotii with a preservation number of CGMCC No.13772 on a corn flour culture medium. The paecilomyces variotii powder has the beneficial effects that (1) paecilomyces variotii can parasitize nematodes and simultaneously produce multiple secondary metabolites so as to inhibit the growth of the nematodes, so that the paecilomyces variotii powder can be used for effectively preventing and treating the nematodes; (2) the paecilomyces variotii powder is high in spore content and nematode killing efficiency and long in guarantee period; and (3) a preparation process of the paecilomyces variotii powder is simple and suitable for industrial production.

The invention discloses a bio-organic fertilizer for killing underground insect pests by using a biological agent and a preparation method of the bio-organic fertilizer. The bio-organic fertilizer is prepared from the following raw materials in parts by weight: 5-15 parts of a beauveria bassiana agent, 10-20 parts of cow dung, 20-30 parts of pig manure, 10-15 parts of chicken manure, 10-15 parts of cassava residues, 5-10 parts of furfural residues and 15-25 parts of sesame-seed cakes. The beauveria bassiana agent comprises the following components in parts by weight: 40-50 parts of a beauveria bassiana spore powder mother drug, 20-30 parts of a beauveria buchneri spore powder mother drug, 20-30 parts of corn flour and 10-20 parts of glucose powder. The preparation method comprises the following steps: (1) preparing a base fertilizer; (2) performing microbial inoculum culture; (3) airing the base fertilizer; and (4) performing microbial agent mixing. The organic fertilizer provided by the invention utilizes a biological agent to kill underground pests, has a long lasting period of pest control, is non-toxic to people, bees, fishes and the like, has no pollution to the environment, has a good disinsection effect and long fertilizer efficiency, and can reduce environmental pollution caused by chemical pesticide disinsection.