91 results about "Brevibacillus brevis" patented technology

The invention relates to broad-spectrum compound microbial bacteria for preventing and treating vegetable fungal diseases, and belongs to the technical field of agricultural biological prevention and treatment. The broad-spectrum compound microbial bacteria is compounded from four microorganisms, namely bacillus subtilis BSD-2 ((i) Bacillus subtilis), brevibacillus brevis ch2 ((i) Brevibacillus brevis), bacillus amyloliquefaciens Y10 ((i) bacillus amyloliquefaciens) and paenibacillus polymyxa Y89 ((i) Paenibacillus polymyxa) according to a certain ratio. By adopting the compound bacteria, the vegetable fungal diseases of a greenhouse or a large field, for example, botrytis, leaf mold, downy mildew, powdery mildew, blight and the like can be effectively prevented and treated, the field efficacy can be up to over 70%, the yield is increased by 5-20%, and the broad-spectrum compound microbial bacteria are significant in economic, social and ecological benefits, and have broad popularization and application prospects.

The invention belongs to the technical field of agricultural biology, and particularly discloses a Rhodococcus ruber XS-2 strain of which the collection number is CGMCC No.11562. The Rhodococcus ruber XS-2 strain has the function of degrading chemical pesticides. The invention also discloses a microbial preparation containing the Rhodococcus ruber and a preparation method thereof. The microbial preparation is compounded from the Rhodococcus ruber, Serratieae Bacillus subtilis and Brevibacillus brevis. The microbial preparation has the functions of restoring pesticide-polluted soil, preventing diseases and promoting growth, has high environmental safety, and has favorable development and application prospects.

The invention discloses a bacillus subtilis H4, a decomposed inoculant prepared therefrom and an application of the decomposed inoculant. The decomposed inoculants is composed of a freeze-dried powder of brevibacillus brevis H3,a freeze-dried powder of the bacillus subtilis H4, a freeze-dried powder of bacillus subtilis H8-3 and starch with a weight ratio being 1:1:1:97. The invention provides a new strain bacillus subtilis H4, the decomposed inoculant prepared from the bacillus subtilis H4 is high-temperature-resistant and can secrete protease and lipase for high-efficiently fermenting animal wastes. Compost prepared through the decomposed inoculant is less in environmental pollution, is economical and is easy to use. When a product after compost treatment is subjected to subsequent treatment, the product can be employed as an organic fertilizer for achieving an object of turning waste into resources.

The present invention relates to the antagonistic bacteria that are used to prevent and eliminate tobacco bacterial wilt and their microbial organic fertilizer, which belong to the technology for agricultural intensive production. The present invention separates two antagonistic bacteria (Brevibacillus brevis NJL-25 and Bacillus cereus NJL-14) that have remarkable antagonistic action against the pathogens of tobacco bacterial wilt. Microbial organic fertilizer is produced from these antagonistic bacteria and organic compost. Wherein, the content of each of NJL-25 and NJL-14 is above 1×10¹⁰ cfu/g, the content of total nitrogen is 4˜5% (above 90% of the nitrogen is organic nitrogen), the content of total nitrogen-phosphorus-kalium nutrient is 6˜10% and the content of organic matter is 30˜35%. As indicated by experiments, after the microbial organic fertilizer is applied into soil, it will enable rapid multiplication of the antagonistic bacteria into a dominant microflora in the soil and achieve more than 97.6% of biocontrol rate of tobacco bacterial wilt in the soil suffering from tobacco bacterial wilt. If the microbial organic fertilizer is applied in the soil without continuous cropping obstacle in a long time, it can prevent the occurrence of the bacterial wilt of continuous cropping tobacco to a great extent.

The invention relates to antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt, which belongs to intensive agricultural production technology. According to the invention, a microbial organic fertilizer is produced from two antagonistic bacteria (brevibacillus brevis NJL-25 and bacillus cereus NJL-14) which have a remarkable antagonistic function on pathogenic bacteria of the tobacco bacterial wilt and are obtained by separation, and organic fertilizers, wherein 1 gram of the microbial organic fertilizer contains more than 1*10<10> NJL-25 and 1*10<10> NJL-14, and has the total nitrogen content of 4 to 5 percent (organic nitrogen content is over 90 percent), the total nitrogen-phosphorus-potassium content of 6 to 10 percent and the content of organic matters of 30 to 35 percent. Tests prove that the microbial organic fertilizer, after being applied to soil, can fast multiply the antagonistic bacteria to form a dominant group in the soil, achieve the biological prevention and control rate of over 97.6 percent on the bacterial wilt in the soil with the tobacco bacterial wilt, and prevent the occurrence of the continuous-cropping tobacco bacterial wilt to a great extent after being applied for long to the soil without continuous cropping obstacles.

The invention discloses a biological agent for compost treatment of livestock manure. The biological agent contains Streptomyces thermocarboxydus, Brevibacillus brevis, Penicillium polonicum and Bacillus licheniformis. When the biological agent is inoculated into a pile body, the temperature of the livestock manure can rise quickly in cold autumn and winter and be kept for a relatively long time, so that the livestock manure can be quickly decomposed and converted into a high-quality organic fertilizer. The biological agent can also be used for mixed compost treatment of the livestock manure and urban and rural agricultural and forestry wastes under the condition of a relatively low ambient temperature.

The invention relates to an antagonistic bacterium for preventing the fungal diseases of broccoli as well as a preparation method and the application of a microbial preparation of the antagonistic bacterium, and belongs to the field of biological prevention and treatment of crop diseases. The invention provides a strain of brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622, a production method of a microbial preparation containing the brevibacillus brevis, and also the application of the brevibacillus brevis BBC-3 or the microbial preparation in preventing and treating the fungal diseases of the broccoli. The brevibacillus brevis BBC-3 with the collection number CGMCC No. 9622 is obtained by virtue of preliminary screening and secondary screening, wherein a plate opposing test is performed for the preliminary screening and a liquid fermentation liquid is adopted for the secondary screening; the brevibacillus brevis BBC-3 is capable of effectively preventing and treating a plurality of fungal diseases of the broccoli, such as gray mold, downy mildew, stalk break, black rot and black spot, and has the prevention and treatment efficiency of above 70%; in addition, the brevibacillus brevis BBC-3 is good in environmental safety and has excellent development application prospect.

The invention discloses a strain which has an antagonistic effect is obtained by separating and screening from watermelon rhizosphere soil in Yongtai County, Fujian Province. The strain is brevibacillus brevis strain FJAT-0809-GLX (Brevibacillus brevis FJAT-0809-GLX). A microbial fresh-keeping bacterium agent which can keep longan fresh is obtained by adopting the fermentation liquor of the brevibacillus brevis strain FJAT-0809-GLX. The strain has the advantages that: a new brevibacillus brevis strain FJAT-0809-GLX is provided; the microbial fresh-keeping bacterium agent which can keep the longan fresh is prepared by the adopting the brevibacillus brevis strain FJAT-0809-GLX; and the bacterium agent can reduce the rotting rate of the longan and can keep the color, the luster and the taste of fruits.

The invention discloses a brevibacillus brevis strain and application thereof. The strain is brevibacillus Bb-1, and is preserved in CGMCC (China General Microbiological Culture Collection Center); the preservation number is CGMCC NO.12365. The invention also provides application of an antibiological inoculants prepared from the brevibacillus brevis Bb-1 to preparation and treatment of root-knot nematode. The brevibacillus brevis is separated from soil, has a good prevention effect on the root-knot nematode, can easily realize the fixed planting in the soil, and is favorable for the early stage prevention and control of the root-knot nematode harm. The strain has huge application potential on the problem solving of the prevention and the treatment of the root-knot nematode, and is favorable for realizing the high-yield safe and harmless production of vegetables; the killing rate of the fermentation liquid of the brevibacillus brevis Bb-1 strain on the root-knot nematode reaches 100 percent; the prevention and treatment effect in the potted plant experiments reaches 81.6 percent; the prevention and treatment effect is high and stable; the agricultural production requirements are met.

The invention discloses brevibacillus brevis H3 and application thereof to prepare a composting inoculant. The brevibacillus brevis H3 is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: M2013481, the preservation data is 21rt, October, 2013, and the address is Wuhan University, Wuhan City, Hubei province. The brevibacillus brevis H3 is resistant to high temperature, is survival at a temperature scope of 30 DEG C-60 DEG C, and is relatively high in lipase activity and relatively rapid in growth. The composting inoculant prepared by utilizing the bacterial strain has the advantages of high temperature resistance and capability of secreting protease and lipase by efficiently fermenting animal wastes. By utilizing the composting inoculant with the advantages of being small in environmental pollution, economic, practicable and the like, after a product subjected to compost processing is subjected to subsequent processing, the processed product can be used as an organic fertilizer and the purpose of changing waste into valuables is reached.

The invention relates to brevibacillus brevis for preventing and treating plant fungous diseases and application thereof. The brevibacillus brevis has a gene sequencing result of 1367bp, has an accession number of HQ585418 on a gene bank, is preserved in the China general microbiological culture collection center (CGMCC) and has a preservation number of CGMCC NO.4361. A method for preparing a brevibacillus brevis biopesticide for preventing and treating the plant fungous diseases comprises the following operation steps of: 1, preparing seed liquid; and 2, fermenting and preparing the brevibacillus brevis biopesticide. A field experiment proves that the brevibacillus brevis can obviously reduce the morbidity of cucurbit wilt, and thus, a strain disclosed by the invention can be effectively applied to prevention and treatment of cucurbit wilt and can be used for preparing the safe and high-efficiency biopesticide.

The invention relates to a strain which is separated and screened from pinellia ternate rhizosphere soil in Dafang county of Guizhou province and has the antagonism. Through authentication of the microbial taxonomy, the strain is named as the brevibacillus brevis strain GZDF3, and biological fungicide capable of preventing and treating pinellia ternate block stem rotten sickness is obtained by adopting fermentation liquor of the strain. The strain is obviously characterized in that the new brevibacillus brevis strain GZDF3 is provided; in addition, the antibiological inoculant prepared through the fermentation liquor of the strain has the obvious antagonism on pathogenic bacteria and pathogenic fungus, such as fusarium oxysporum and fusarium solani, of the pinellia ternate block stem rotten sickness; and the strain is an important microbial resource on the traditional Chinese medicinal material green planting industry.

The invention relates to a brevibacillus brevis TW effervescent granule. The brevibacillus brevis TW effervescent granule is prepared through the following steps of: mixing a brevibacillus brevis TW strain single colony plaque PDA (Potato Dextrose Agar) liquid culture solution with soybean meal, corn meal, starches, cane sugar, yeast powder, calcium carbonate and MnSO4 by adding water; mixing bacterial fermentation liquor obtained by fermenting under the conditions of 100% dissolved oxygen, the pH of 7.0-7.2 and the temperature of 30 DEG C with the cane sugar, polyvinyl alcohol, citric acid monohydrate, potassium tartrate and sodium bicarbonate according to a proportion; screening, granulating, and drying through steam flows at a temperature of 60 DEG C, wherein the total concentration ofviable bacteria of a finished product is about 1*10<9>-1*10<10> cfu/g. The brevibacillus brevis TW effervescent granule can be effectively suspended on a water surface and then fast disintegrated, releases the biocontrol bacteria of living bodies and enables the biocontrol bacteria to attach to the contact surface of the stem of a rice plant and the water surface, thereby effectively cutting off or spatially occupying the initial invasion of rice rhizoctonia solani on the rice stem and achieving the purpose that control effect on rice sheath blight reaches more than 80 percent; and the brevibacillus brevis TW effervescent granule is convenient for application, has low cost and can be preserved for 2-3 years at normal temperature.

The invention discloses a brevibacillus brevis strain Brevibacillus brevis A1, and the conservation serial number in the general microbiology center of the China committee for culture collection of microorganisms is CGMCC NO.11148. Feather meal is used as a unique carbon and nitrogen source of the brevibacillus brevis strain, a fermentation medium with a complete feather is inoculated with the strain, after the strain is fermentally cultured for 48 h, most of the feather and barbule is degraded, and the effect of degrading feather keratin is remarkable; the optimum action temperature of keratinase generated by the strain is 55-60 DEG C, keratinase is medium-temperature andneutral, the optimum pH value is 7.0, and the brevibacillus brevis strain can be applied to related production field of feather degradation.

The invention relates to endogenous brevibacillus brevis wettable powder which is formed by brevibacillus brevis B-011 (with the collection number of CGMCC No.7466) fermentation liquor, talcum powder, calcium lignosulphonate, DBS (Sodium Dodecyl Benzene Sulfonate), CMC-Na (sodium carboxymethylcellulose), dextrin and white carbon black according to a certain proportion. The prepared brevibacillus brevis B-011 wettable powder achieves the spore content at 1.8*1010 cfu/g at the room temperature of 25 DEG C, the pH value at 6.8, the suspension rate at 76%, the wetting time at 52 seconds and the fineness pass rate at 98%, achieves the survival rate by 90% after being stored at (4+/-2) DEG C for 100 days, plays prevention and control roles on tomato early blight, can effectively reduce the usage ratio of a chemical pesticide on agricultural production, alleviates the destruction of a pesticide on an ecological environment and meets the requirements of the modern society on the agricultural production and IPM (Integrated Pest Management).

The invention provides a plant growth-promoting rhizobacteria strain Gxun-20 and application thereofin plant growth promotion. A bacterial strain with efficient IAA secretion, phosphorus dissolving and growth promoting capabilities is selected out through an ADF screening culture medium and an LB purification culture medium in a screening mode, 16S rRNA and physiological and biochemical characteristics of the bacterial strain are determined, the bacterial strain is determined to be brevibacillus brevis, the bacterial strain is gram-positive bacteria, and the bacterial colony morphology is round, smooth, convex and white. The strain is preserved in the Guangdong Microbial Culture Collection Center on June 13th, 2019. The fermentation condition of Gxun-20 is optimized, and the bacterial liquid obtained by fermentation is used for carrying out a growth-promoting test on plants. Compared with other strains, the strain Gxun-20 has higher IAA yield, and can be used for promoting the growth and development of plants, so that the use of chemical fertilizers and the damage of chemical fertilizers to soil are effectively reduced.

The invention discloses an oil field wastewater treatment process which comprises the steps: firstly, adding oil field wastewater in a buffer pond, adding the oil field wastewater in a negative pressure cooling tower through a lifting pump for being cooled, sequentially carrying out hydrolytic acidification in a primary anoxia pond, nitration treatment in a primary aerobiotic pond and precipitation in a precipitation pond on the cooled wastewater, adding the wastewater in an MBR, discharging the wastewater when reaching the standard, wherein microorganisms adopted in processes of hydrolytic acidification treatment, nitration treatment and denitrification treatment comprise brevibacterium flavum, bacillus cereus, pseudomonas stutzeri and brevibacillus brevis. According to the oil field wastewater treatment process, through improvement of an oil field wastewater treatment system and process, the oil field wastewater treatment efficiency can be further increased, and the effluent quality is improved; the two selected strains have a synergic effect when being used for commonly treating the oil field wastewater, and are capable of effectively degrading pollutants in the oil field wastewater.

The invention discloses a gene engineering strain for recombinant expression of Bacillus stearothermophilus alpha/beta-CGT enzyme and application of the gene engineering strain, belonging to the fields of gene engineering and enzyme engineering. According to the invention, the recombinant plasmid p SVEB-alpha/beta-CGTase is constructed, and Bacillus pumilus Brevibacillus brevis (CCTCC AB 94025) is transformed, so that recombinant Bacillus pumilus is obtained; based on the recombinant Bacillus pumilus as the strain, fermentation is carried to produce an alpha/beta-CGT enzyme which is applied to preparation of beta-cylodextrin and has good effect. The gene engineering strain for recombinant expression of Bacillus stearothermophilus alpha/beta-CGT enzyme disclosed by the invention has the advantages that the food-safe Bacillus pumilus is adopted as an expression host to recombine and produce alpha/beta-CGT enzyme, the recombinant strain is high in enzyme production, the ferment materials are wide in resources, and the production cost is relatively low.

The invention relates to the fields of biopesticide, and specifically discloses a Brevibacillus atrophaeus wettable powder containing a spore germinator, and a preparation method thereof. The wettable powder consists of the following components by weight: 40-70 parts of a Brevibacillus atrophaeus fermentation broth, 20-30 parts of a kaolin filler, 1-4 parts of a wetting agent sodium lauryl sulfate, 0.2-1 part of a dispersant of Morwet D-425, a spore germinator containing 0.1-1 part of fructose, 0.1-1 part of threonine and 0.1-0.5 part of pyridine-2,6-dicarboxylic acid calcium, a UV protective agent containing 1-2 parts of mycose and 0.2-1 part of carboxymethyl cellulose, and a drying protecting agent containing 1-5 parts of cane sugar and 0.2-1 part of monosodium glutamate. After application of the Brevibacillus atrophaeus wettable powder into the field, the spores have high germination speed and high germination rate; and the control effect of the Brevibacillus brevis wettable powder can be significantly improved, the usage amount of spores and the production costs can be reduced.

The invention relates to the fields of biopesticide and microbial fertilizer, and specifically discloses a Brevibacillus brevis wettable powder containing a spore germinator, and a preparation method thereof.. The wettable powder consists of the following components by weight: 40-70 parts of a Brevibacillus brevis fermentation broth, 20-30 parts of a white carbon black filler, 1-4 parts of a wetting agent sodium lauryl sulfate, 0.2-1 part of a dispersant of T lignin, a spore germinator containing 0.1-0.5 part of inosine and 0.1-0.5 part of fructose, a UV protective agent containing 0.5-2 parts of lecithin and 0.2-1 part of beta-dextrin, and a drying protecting agent containing 1-4 parts of maltose and 0.2-1 part of sucrose. After application of the Brevibacillus brevis wettable powder into the field, the spores have high germination speed and high germination rate; and the fertilizer efficiency and control effect on plant disease of the Brevibacillus brevis wettable powder can be significantly improved, the amount of application and the production costs can be reduced.

The invention belongs to the technical field of farm chemicals, and relates to a composition comprising brevibacillus brevis TW and trichodermin for preventing soil-borne diseases of melons, fruits and vegetables. The composition is wettable powder, which comprises 1 billion to 100 billion CFU/g of brevibacillus brevis TW and 10 million to 10 billion CFU/g of trichodermin; and the composition also comprises other ingredients in percentage by weight such as: 1 to 40 percent of dispersant, 1 to 40 percent of wetting agent and the balance of filling materials. The composition has the advantages that: (1) a good effect on preventing verticillium wilt and root rot of strawberries and blight and damping-off of vegetables can be realized; (2) the lasting period for the verticillium wilt and root rot of strawberries and the blight and damping-off of vegetables is long, so the times of the drug application can be reduced, and the prevention cost can be reduced; and (3) the composition is micro-organism combined preparation, has no chemical residue and is safe to use.

The invention relates to brevibacillus brevis and application thereof, and belongs to the technical field of microbial pesticides and microbial fertilizers. A strain DLO1 of brevibacillus brevis has been collected in China General Microbiological Culture Collection Center on June 16, 2020; the address is No.3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; the collection number is CGMCC No.20096. Microbial cells of brevibacillus brevis and metabolites of the microbial cells can be mixed with a conventional pesticide adsorption carrier and an auxiliary agent to prepare a DL01 bacterial agent. The invention further relates to application of the DL01 strain to preparation of the bacterial agent for controlling the root knotnematode. The bacterial agent prepared by the DL01 strain can effectively control the root knot nematode of traditional Chinese materials, and has the characteristics of being low in cost, high in control efficiency, having no residues, and being safe to humans and animals.