22 results about "Brevibacillus brevis" patented technology

The invention relates to a method for in-situ remediation or management and control of a petroleum contaminated site by micro-nano aeration enhanced microorganisms, which comprises the following steps: 1) injecting micro-nano bubbles into soil or underground water, and continuously aerating for 3-5 days for 6-10 hours every day; an air source of the micro-nano bubbles is one or a combination of two of air and oxygen; 2) 12-24 hours after the step 1) is completed, a compound microbial agent is injected into the soil or the underground water, the compound microbial agent comprises compound microorganisms, and the compound microorganisms comprise pseudomonas, leucas, phenyl bacilli and brevibacillus in the viable count ratio of (1-25): (1-15): (1-5): 1. According to the method, the energy consumption is low, secondary pollution is avoided, the remediation or control effect is excellent, and the total petroleum hydrocarbon (C10-C40) pollutant concentration of the contaminated site after remediation is remarkably reduced.

The application provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method thereof, wherein the compound microbial agent comprises Brevibacillus brevis, Pseudomonas chlororaphis and Streptomyces; wherein the ratio of the viable bacterial count of the Brevibacillus brevis, the Pseudomonas chlororaphis and the Streptomyces is (1-3):(2-4):(1-2). The Brevibacillus brevis directly inhibits bacteria by secreting antibacterial peptides, the Pseudomonas chlororaphis competes for ecological niches through iron carriers, and the Streptomyces produces antibiotics, so that multiple strains form a "bacteriostasis-competition-immune activation" multiple mechanism, thereby achieving efficient prevention and treatment of bacterial diseases of fruits and vegetables.

The application discloses a plant-source bio-growth-promoting synergistic fertilizer and a preparation method thereof. The fertilizer is prepared by compounding plant-source composite growth-promoting substances, a composite microbial flora and a carrier material in a specific mass ratio. The plant-source composite growth-promoting substances are prepared by solid-state fermentation and extraction of gynostemma pentaphyllum, astragalus and acanthopanax. The composite microbial flora contains brazilian nitrogen-fixing spirillum, fluorescent pseudomonas and brevibacillus brevis, and is embedded and fixed by using kappa-carrageenan microcapsules containing biochar nanoparticles. The carrier is lignosulfonate-modified nano-attapulgite. The application also provides a corresponding preparation method. The granular fertilizer is finally prepared through steps of activating the strains by a composite activation solution, embedding by microencapsulation and carrier loading granulation. The fertilizer combines the synergistic effect of plant-source active substances and functional microorganisms, significantly improves the activity of the flora and the field colonization capacity, and can effectively promote plant growth, improve crop yield and quality.

The invention relates to a strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 and an application of the strain of biocontrol and growth-promoting brevibacillus brevis LJQJ21 in prevention and treatment of plant oomycetes diseases, and belongs to the field of research and development of microbial pesticides. The Brevibacillus formis LJQJ21 has the capabilities of promoting growth and preventing diseases, has a relatively strong inhibition effect on mycelial growth of various pathogenic oomycetes (phytophthora capsici, phytophthora infestans, peronophythora litchii and pythium aphanidermatum) causing crop diseases, and can secrete pentapeptide antibacterial substances Edeine A and B, and the Edeine can remarkably inhibit germination of zoospores of the phytophthora capsici. In addition, the microbial agent taking the LJQJ21 spore fermentation bacterial liquid as a main active component has a relatively good prevention and treatment effect on fruit rot caused by phytophthora capsici. The Brevibacillus forts LJQJ21 has obvious growth promoting and disease preventing effects, is safe to human and livestock and environment-friendly, and has potential field application value and development prospect.

The present invention relates to a Brevibacillus ( Brevibacillus brevis ) ZLP‑152, with the deposit number CGMCC No. 32228, can continuously produce alkali throughout the growth cycle and antagonize various pathogens such as cotton red rot. The composite bacterial agent formed by it, phosphate-solubilizing bacteria HM0332 and silicate bacteria HM8841 can improve soil acidification and / or soil microecological environment.

The application discloses a strain of Brevibacillus sp. HNCS-1, relates to the technical field of tea tree planting, and has a preservation number of CCTCC NO: M2022713. The strain is classified and named as Brevibacillus sp., and is preserved in the China Center for Type Culture Collection on May 25, 2022, and is located at Wuhan University, Wuhan, China, and has a postal code of 430072. The strain shows significant inhibitory effects on common pathogenic bacteria in tea gardens, such as tea anthracnose bacteria, tea leaf penicillium, tea fusarium, tea white star bacteria, tea round brown spot disease, and tea wheel spot disease, is environment-friendly, and is non-toxic and non-harmful.

The invention discloses a brevibacterium casein peptide A derivative compound and application thereof, the brevibacterium casein peptide A derivative is a natural compound generated by brevibacterium brevis B011, the structural general formula of the brevibacterium casein peptide A derivative is cyclo-(DFPLDYNQYVOL), the mass-to-charge ratio m / z of the brevibacterium casein peptide A derivative is 1236.6789, and the accurate molecular weight of the brevibacterium casein peptide A derivative is 1235.6745. The derivative is purified through semi-preparative liquid chromatography, and a plate bacteriostasis test result shows that the minimum inhibitory concentration of the derivative to valsa bergamot pear germs is lower than that of tyrocidine A, so that the derivative has good bacteriostatic activity and application and development prospects. The invention also discloses a method for exploring the derivative compound.

The invention provides bacillus brevis HDCC00067, the preservation number of the bacillus brevis HDCC00067 is CGMCC (China General Microbiological Culture Collection Center) No.35553, and the capability of producing brevibacterium peptide A is obviously higher than that of the prior art. The applicant also finds that the yield of the brevibacitracin A can be obviously improved when amino acid precursor substances are added into the fermentation culture medium, especially when the concentrations of L-tryptophan, D-leucine and D-valine are controlled to be within a certain range. By controlling pH and dissolved oxygen in a tank fermentation culture medium and optimizing a feeding control strategy of a mixed solution of a carbon source and a nitrogen source, the content of the brevibacitracin A in fermentation liquor is further greatly increased and reaches 7.5 g / L or above, and the brevibacitracin A can be used for large-scale industrial production.

The present application relates to the field of environmental microorganism and wastewater treatment technology, and specifically discloses a kind of fermentation microbial inoculum for sewage treatment and application.The fermentation microbial inoculum is made by immobilizing multifunctional composite microbial inoculum on chitosan magnetic microspheres;The multifunctional composite microbial inoculum is compounded by the fermentation broth of Pseudomonas koreensis, Lactobacillus acidophilus, Brevibacillus brevis, Timumasidium and Bacillus licheniformis according to a specific viable count ratio.The chitosan magnetic microspheres have Fe3O4 as magnetic core and are coated with crosslinked chitosan layer.The present application successfully constructs a microbial repair system with functional complementation and easy recovery by combining specific flora compounding with magnetic immobilization technology.The microbial inoculum can efficiently and synchronously remove nitrate, tetrabromobisphenol A and heavy metal multi-component pollutants in groundwater or contaminated water bodies, and can be quickly recovered and reused by external magnetic field, with the advantages of high treatment efficiency, environmental friendliness and low cost.

The invention provides a compound microbial agent for preventing and treating bacterial diseases of fruits and vegetables and a preparation method of the compound microbial agent. The compound microbial agent comprises brevibacillus brevis, pseudomonas chlororaphis and streptomyces. Wherein the ratio of the viable count of the brevibacillus brevis to the viable count of the pseudomonas chlororaphis to the viable count of the streptomyces is (1-3): (2-4): (1-2). According to the invention, brevibacillus brevis secretes antibacterial peptides for direct bacteriostasis, pseudomonas chlororaphis competes for ecological niche through siderophores, streptomycete generates antibiotics, and multiple strains cooperate to form multiple mechanisms of bacteriostasis-competition-immune activation, so that high-efficiency prevention and treatment of fruit and vegetable bacterial diseases are realized.

The invention relates to the technical field of microorganisms, in particular to a microbial composition, a complex microbial inoculant and application. The microbial composition disclosed by the invention comprises bacillus amyloliquefaciens, brevibacillus brevis and trichoderma harzianum, and can be used for simultaneously preventing and treating rice blast in a rice seed production process and aspergillus flavus pollution in a storage process, so that the full life cycle protection of the rice seeds from production to storage is realized; the problem that in the prior art, different agents need to be used for preventing and treating rice blast and aspergillus flavus pollution is solved. In addition, the complex microbial inoculant containing the microbial composition is safe, efficient and environment-friendly, can replace chemical pesticides, reduces environmental pollution and pesticide residues, is simple in preparation method and easy for industrial production, and has a wide application prospect.

The present invention provides a Brevibacillus formosus strain SXM-17, its derivative products and applications in preventing and controlling crop diseases, belonging to the technical field of functional microorganisms. In the present invention, a strain SXM-17 is isolated and purified from wheat plants, and the preservation number is CGMCC No. 30198, which has good inhibitory effects on Gibberella zeae, Physalospora piricola, Botrytis cinerea, Alternaria solani, Rhizoctonia solani and Gaeumannomyces graminis var. tritici, etc. Experiments show that the culture solution, bacterial suspension and supernatant of the strain SXM-17 all have good control effects. In addition, the strain SXM-17 also has a growth-promoting effect on crops planted in natural planting environments and salinized environments. The strain SXM-17 provided by the present invention enriches the resource library of biocontrol strains against Gibberella zeae and the resources of green and efficient biological control microorganisms.

The invention relates to the technical field of environmental microorganisms and wastewater treatment, and particularly discloses a fermentation inoculant for sewage treatment and application. The fermentation inoculant is prepared by immobilizing a multifunctional composite inoculant on chitosan magnetic microspheres, the multifunctional complex microbial inoculant is prepared by compounding fermentation broth of pseudomonas koreensis, lactobacillus acidophilus, bacillus brevis, massinia pemophila and bacillus licheniformis according to a specific viable count ratio, wherein the fermentation broth of the pseudomonas koreensis, the lactobacillus acidophilus, the bacillus brevis, the massinia pemophila and the bacillus licheniformis are used as raw materials. Fe3O4 is used as a magnetic core of the chitosan magnetic microsphere, and a cross-linked chitosan layer is coated outside the magnetic core. Through combination of specific flora compounding and a magnetic immobilization technology, a microbial remediation system which has complementary functions and is easy to recover is successfully constructed. The microbial agent can efficiently and synchronously remove nitrate, tetrabromobisphenol A and heavy metal multi-component composite pollutants in underground water or polluted water, can be quickly recycled and reused through an external magnetic field, and has the advantages of high treatment efficiency, environment friendliness and low cost.

Provided are compositions derived from Beauveria bassiana, Brevibacillus brevis, Streptomyces spororaveus, Paenibacillus elgii, or Streptomyces sp. fermentation, having algicidal properties, and method of use thereof.

The present invention provides a method for improving the pollination rate and pod-setting rate of alfalfa, belonging to the field of biotechnology. The present invention provides a strain of salt-tolerant Brevibacillus brevis ( Brevibacillus halotolerans ) EB3, with the preservation number of CGMCC No. 33617. Further provided are a bacterial agent composition composed of this bacterium and marine yeast, a composition composed of the bacterial agent composition and pollen nutrient solution, and a method for improving the pollination rate and pod-setting rate of alfalfa by using this composition, effectively solving the problems of low seed setting rate, small flower abortion, serious flower dropping, etc. of alfalfa, significantly increasing the pollen quantity and pollen germination rate of alfalfa, reducing the small flower dropping rate, thereby achieving the effect of significantly increasing the seed yield of alfalfa, and the method is simple to operate, with stable effects and low cost.

The purpose of the present invention is to provide a composition that contains, as an effective microbe, a microorganism having the effect of inhibiting the onset of plant diseases, that is usable as a biotic pesticide, and that is for use in controlling plant diseases.　The composition is for use in controlling plant diseases, and contains, as an active ingredient, at least one selected from the group consisting of cells of a Brevibacillus formosus strain NBRC 15716 or a mutant strain thereof, or a culture thereof, and cells of a Brevibacillus brevis strain NBRC 110488 or a mutant strain thereof, or a culture thereof.

The invention discloses saccharomyces cerevisiae engineering bacteria capable of efficiently expressing dye decolorizing peroxidase and application of the saccharomyces cerevisiae engineering bacteria. The brevibacillus brevis BaDyP is heterologously expressed in saccharomyces cerevisiae, a strain BLWJ001 capable of synthesizing the BaDyP is constructed, the strain BLWJ001 is taken as an original strain, Hem14, Hem15, Hem2, Hem13, Hem1, PUG1, SPS22, REE1, PHO84, CLB2 and Hem4 are knocked in or delta shm1, delta gcv1 and delta HMX1 are knocked out, the heme yield is increased, the decolorization rate of the engineering strain for dyes is greatly improved, and a solid foundation is laid for research and application of decolorization in the fields of food, environment, chemical engineering and the like. In addition, rational design and site-directed mutagenesis modification are carried out on BaDyP, so that the catalytic performance and stability of BaDyP are improved.

The present invention relates to the field of alfalfa cultivation, and particularly to a synthetic microbial community that breaks the dormancy of alfalfa seeds and promotes the growth of seedlings and its application. The present invention provides a strain of Sinorhizobium meliloti NFB5 with the preservation number of CGMCC NO. 33618, which can break the dormancy of alfalfa seeds and promote the growth of alfalfa seedlings and roots. The present invention combines Sinorhizobium meliloti NFB5 with one or two of the screened salt-tolerant Brevibacillus brevis EB3 and Bacillus sp. PS06 to obtain a synthetic microbial community. By soaking alfalfa seeds in the bacterial liquid containing the synthetic microbial community, the number of hard seeds can be significantly reduced, and the seed germination rate and germination potential can be significantly increased, indicating that the synthetic microbial community can better break the dormancy of alfalfa seeds, and there is no loss to the seeds and the seed quality will not be reduced. In addition, the synthetic microbial community also has a significant growth-promoting effect, which can significantly increase the seedling plant height, root length, stem weight and root weight.

The application discloses a seed coating agent for promoting plant stress resistance and a preparation method and application thereof. The coating agent comprises an A agent, a B agent and a C agent. The A agent comprises phosphate rock powder, potash powder and biochar powder. The B agent comprises Massilia bacteria and / or Brevibacillus brevis. The C agent comprises malt dextrin. The Massilia bacteria has a preservation number of CCTCC NO:M2023996, and the Brevibacillus brevis has a preservation number of CCTCC NO:M2023995. The coating agent can improve the drought resistance or salt stress resistance of plants.

The application discloses a short bacillus tyrocidine derivative and application thereof. The derivative is a secondary metabolite natural product produced by Brevibacillus brevis, and a structural formula of the derivative is as follows: cyclo- (O D FPL D FNQYVOL. The derivative has good bacteriostatic activity on various plant pathogenic fungi, and has practical application value.

The invention provides brevibacillus brevis 9-1 and application thereof in bacterial manure, and belongs to the technical field of microorganisms, the brevibacillus brevis 9-1 is separated from soil, the preservation number of the brevibacillus brevis 9-1 is CCTCC NO: M 20242933, the preservation date is December 30, 2024, the brevibacillus brevis 9-1 is preserved in the China Center for Type Culture Collection, and a 16S rDNA sequence is shown as SEQ ID NO: 1; the brevibacillus sp. 9-1 has the functional activity of solubilizing phosphorus, solubilizing potassium, fixing nitrogen and producing ACC (Acetobacter Cellulose), and the brevibacillus sp. 9-1 has the functional activity of solubilizing potassium, fixing nitrogen and producing ACC; the microbial fertilizer is obtained by enlarged culture of brevibacillus brevis 9-1, and compared with a chemical fertilizer, the microbial fertilizer used in the invention is more environment-friendly, not only can improve soil fertility, but also can promote balance and diversity of soil microbial communities, and is helpful for maintaining health and stability of a soil ecosystem.

The invention belongs to the technical field of oily sludge degradation microbiology, and relates to a petroleum hydrocarbon degrading bacterium, application and an oily sludge degradation method. The petroleum hydrocarbon degrading bacterium is brevibacillus brevis, and the preservation number of the petroleum hydrocarbon degrading bacterium is CGMCC (China General Microbiological Culture Collection Center) No. 28749. The petroleum hydrocarbon degrading bacteria are extracted from the oily sludge, the extraction cost is low, and the method is simple and feasible. Petroleum hydrocarbon degrading bacteria are prepared into bacterial liquid with OD600 of 1, 50 mL of the bacterial liquid is sprayed to each kg of oily sludge, high-temperature aerobic fermentation is performed on the oily sludge under the aerobic conditions that the pH is 7-7.5 and the temperature is 50-70 DEG C, the oil removal rate reaches 38.2% within 7 days, the degradation effect is remarkable, and theoretical reference is provided for shortening the period of treating the oily sludge through a biological method and improving the treatment efficiency.