71 results about "Paenibacillus validus" patented technology

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The invention discloses paenibacillus polymyxa K17 for preventing and treating diseases, the preservation number of the paenibacillus polymyxa K17 is CGMCC (China General Microbiological Culture Collection Center) No.33366, and the paenibacillus polymyxa K17 is classified and named as Paenibacillus polymyxa K17. The strain has a good anti-antagonism effect on 16 different plant pathogenic fungi from 8 crops; particularly, the antibacterial effect on various leaf spot diseases of tea trees is superior to that of part of common biocontrol strains such as Pristeria aryabhattai 11, Bacillus tequilensis R2 and the like. The control effect on tea tree leaf spot and tea tree anthracnose is higher than 70%, the control effect on black spot of picked tomatoes is 68%, the control effect is equivalent to that of various chemical pesticides, and the method has extremely high application prospects and market value in field biological control and postharvest preservation of plant diseases. Meanwhile, the strain K17 has the plant growth-promoting characteristics of secreting multiple extracellular hydrolases, dissolving inorganic phosphorus and mineral potassium and biologically fixing nitrogen, and has the potential of preparing plant growth-promoting microbial agents or bacterial fertilizers.

The invention relates to a decomposition-promoting phosphorus-dissolving deodorizing microbial agent for composting and a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, paenibacillus zerzeri C-A and bacillus chestnut brown SC in a mass ratio of 1.5: (1-3): (1.5-4). The viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 1 * 10 < 9 > CFU / g. The preparation method comprises the following steps: (1) strain activation; (2) strain amplification culture; (3) bacterial powder preparation; and (4) strain compounding. The invention relates to application in clean composting of organic wastes. The invention has the following advantages: the three strains in the microbial inoculum do not generate antagonism, and can obviously accelerate the conversion of insoluble phosphorus, promote decomposition and improve the effect of removing NH3 and H2S.

The invention relates to the technical field of plant tissue culture seedling raising, in particular to paenibacillus feed and application thereof in promoting cunninghamia lanceolata test-tube plantlet height growth. The feed paenibacillus paenibacillus SY0317 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO: 20575, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation time is August 31, 2020. The cunninghamia lanceolata test-tube plantlet cultured by adopting the strain has high growth speed, the growth amount is increased by more than 60%, the culture time of the cunninghamia lanceolata tissue culture plantlet is shortened, large-scale production of the cunninghamia lanceolata tissue culture plantlet can be realized, and the strain has remarkable economic and social benefits.

The invention discloses paenibacillus sp. H31, which is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20252669, and the preservation date is November 24, 2025. The invention further discloses a preparation method of the paenibacillus sp. The paenibacillus H31 disclosed by the invention not only has broad-spectrum disease-resistant activity on pathogenic fungi of various plants, but also has a remarkable growth-promoting effect on pepper, so that the paenibacillus H31 can be widely applied to prevention and treatment of agricultural diseases and insect pests, and sustainable development of green agricultural production is promoted. In addition, the paenibacillus H31 also has the effects of hydrolyzing proteinase and cellulose and decomposing ferritin, so that the paenibacillus H31 also can be applied to the scenes of industrial production and the like.

The invention relates to the technical field of microbial soil remediation, in particular to a microbial composition for remediation of saline alkali soil, a microbial agent and application of the microbial agent. According to the characteristics of the bacillus subtilis KC000 and the paenibacillus mucilaginosus JDY001, different compounding proportions are set and applied to the saline-alkali soil, then the salt content of the saline-alkali soil is measured, and it is found that the soil nutrition and environmental conditions are obviously improved by applying the microbial agent, and the saline-alkali soil can be applied to the saline-alkali soil. The soil conditioner is beneficial to the growth and reproduction of paenibacillus mucilaginosus and bacillus subtilis in soil, is beneficial to improving the content of organic matters, rapidly available phosphorus and rapidly available potassium in the soil, increases the content of alkali-hydrolyzable nitrogen in the soil to a certain extent, reduces the pH value of the soil, accelerates salt leaching, inhibits salt return and reduces the salt content of the surface layer of the soil. Meanwhile, the microbial composition also has the effect of preventing and treating sheath blight.

The invention discloses paenibacillus alvei NNUB1, a fungicide and application of the paenibacillus alvei NNUB1. The paenibacillus alvei NNUB1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.28005. The invention further discloses a preparation method of the paenibacillus alvei NNUB1. The newly separated paenibacillus alvei NNUB1 has excellent activity of resisting soil transmission plant pathogens, not only can antagonize various fusarium fungi, but also can effectively inhibit lauraceae lauraceae; the strain also has the characteristic of promoting plant growth, and the biomass of plants can be obviously improved; test data show that the strain can effectively inhibit soil-borne pathogens of continuously-cultivated soil, remarkably promote crop growth and has an excellent application prospect.

The invention discloses a strain of paenibacillus volcano ZTUXWS1 derived from excrement of wild black neck crane and application of the strain, and belongs to the field of microorganisms, the strain is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 20242603. According to the present invention, a culture medium is selected through cellulose, and the Paenibacillus cinereus ZTUXWS1 is obtained by carrying out strain separation on the part, not in contact with the environment, in the fresh faeces of wild birds by using the cellulose to provide the only carbon source, such that the Paenibacillus cinereus ZTUXWS1 can survive in the intestinal tract and can provide the related effect; meanwhile, a nematode toxicity sensitivity test proves that the compound has no obvious toxic effect on nematodes and can be used for degrading cellulose.

The present invention discloses a method for reducing and fixing Cr(VI) by key functional metal-reducing microorganisms based on a mutualistic symbiotic model: S1, preparing a mixed functional bacterial community electrolyte consisting of conductive bacteria and metal-reducing functional bacterial communities, wherein the conductive bacteria is Shewanella, and the metal-reducing functional bacterial communities include one or more of Bacillus cereus, Ralstonia pietermaritzii, and Paenibacillus alvei; S2, enriching an electroactive biofilm: pouring the prepared mixed functional bacterial community electrolyte into an electrolytic cell in a three-electrode bioelectrochemical system, and applying a positive potential of 100 to 300 mV; S3, reducing and fixing Cr(VI): after generating a mature anode biofilm with a stable current output, pouring out the electrolyte in the electrolytic cell, adding a new electrolyte containing Cr(VI), and applying a negative potential of 500 to 0 mV to the working electrode, so that the Cr(VI) in the electrolyte is reduced and fixed.

The present invention belongs to the technical field of maize disease control methods, and particularly relates to a biological bactericide for controlling maize ear rot, its preparation method and application. The biological bactericide comprises a water dispersible granule carrier mixture and a biocontrol bacteria culture solution, and the mass ratio of the water dispersible granule carrier mixture to the biocontrol bacteria culture solution is 2-3:1; the water dispersible granule carrier mixture comprises diatomaceous earth, chitosan and sucrose, and the mass ratio of diatomaceous earth, chitosan and sucrose is 1-2:1-2:1; the biocontrol bacteria culture solution is obtained by mixing fermentation broths of Bacillus subtilis, Streptomyces microflavus, Paenibacillus polymyxa and Bacillus velezensis. The biocontrol bactericide provided by the present invention has a very significant effect on controlling maize ear rot caused by Fusarium verticillioides, has the potential to be used as a new biological pesticide for controlling ear rot caused by Fusarium verticillioides in maize production, and has the value of further research.

The invention provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae, and a method for efficiently preparing rare ginsenoside F2 by using an enzyme method and a whole-cell method, and particularly provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae and a method for efficiently preparing rare ginsenoside F2 by using a beta-glucosidase mutant from Paenibacillus phyllosphaerae. The method comprises the following steps: engineering-modifying beta-glucosidase by utilizing computer-aided design, constructing a mutant library, carrying out primary screening and secondary screening to obtain two high-activity mutants Q7D and G189A, constructing a combined mutant Q7D / G189A, transforming the combined mutant Q7D / G189A into genetically engineered bacterium escherichia coli for expression, and producing F2 by utilizing a whole-cell method. Finally, the highest yield of 18.01 g / L is achieved after the reaction is carried out for 10 h in a 5 L fermentation tank and is 6.82 times that of a shake flask, and the purity of F2 reaches up to 82.20% after the reaction is carried out for 24 h finally. The invention not only provides an efficient solution for large-scale production of rare saponin F2, but also provides a transferable framework for designing and engineering diversified glycoside hydrolase in customized biological catalysis.

The invention discloses subterranean intergeneric bacillus 52-6 with a phosphate solubilizing function and application thereof, and belongs to the technical field of microorganisms. The invention provides subterranean intergeneric bacillus, which is classified and named as subterranean intergeneric bacillus, and the preservation number of the subterranean intergeneric bacillus is CGMCC (China General Microbiological Culture Collection Center) No.32719. The subterranean intergeneric bacillus is named as subterranean intergeneric bacillus. The bacterial colony of the subterranean intergeneric bacillus 52-6 is round, light coral red, semitransparent, flat in surface and tidy in edge, the suitable growth temperature is 35-38 DEG C, the delay phase is short, the growth is rapid, and the culture is easy; the subterranean intergeneric bacillus 52-6 can efficiently dissolve phosphate insoluble in soil and promote absorption of plants to soil phosphorus elements, has the effect of promoting plant growth, effectively improves soil fertility and reduces use of chemical fertilizers, and has huge potential in application of promoting plant growth and improving soil fertility.

The present invention relates to: a composition and a method for biologically controlling plant or crop diseases, the composition comprising three antagonistic microorganisms such as Bacillus velezensis CT-1, Bacillus amyloliquefaciens PT-1, and Paenibacillus polymyxa BW-1. The germination ability of pathogenic conidia that causes infection by adhering to and inhabiting flowers (floral organs) is suppressed by treating with a culture medium or extract of the novel strain, whereby the proliferation of pathogens is suppressed or the infiltration of bacteria into tissues is blocked, and thus the present invention can be effectively used for the stable production and environmentally friendly quality maintenance of fruits.

The present disclosure provides a Paenibacillus mucilaginosus LT1906 and use thereof, and a microorganism-activated circulating fluidized bed (CFB) fly ash-based cementitious material and a preparation method and use thereof, belonging to the technical field of cementitious materials. The Paenibacillus mucilaginosus LT1906 has a deposit number of CGMCC No. 21337. The strain is capable of dissolving silicate minerals and aluminosilicate minerals in the CFB fly ash to increase dissolution of active SiO2. The strain is used for activating the CFB fly ash-based cementitious material, to ameliorate a large water demand and a low early strength of the cementitious material, thereby improving comprehensive utilization of the CFB fly ash in the field of building materials.

This invention belongs to the field of bio-fermentation technology and relates to a strain of Bacillus videmannii SXZY-01 and its applications, including: isolating and screening a strain of Bacillus videmannii capable of producing tanninase from the surface of tobacco. Bacillus wiedmannii The strain SXZY-01, with accession number CCTCC NO:M 2025151, was used to ferment a biological agent containing SXZY-01. After fermentation optimization, the tannin enzyme activity reached 40.98 U / mL. This invention applies a high-enzyme-activity solution to tobacco leaf fermentation and optimizes the fermentation process, achieving efficient degradation of tannins, significantly improving the sensory quality of tobacco, reducing the bitter aftertaste, and demonstrating outstanding technical effects. It provides a green and environmentally friendly solution for upgrading tobacco quality and is in line with the industry's high-quality development direction.

The present disclosure provides a Paenibacillus strain, and a hemostatic polysaccharide produced by same and use thereof. In the present disclosure, a Paenibacillus sp. 1229 has a deposit number of CCTCC NO: M 2022553. A produced exopolysaccharide (EPS) Hemoadhican has a hemostatic function, and can be prepared as a hemostatic material in different dosage forms for different types of hemostasis. The hemostatic material has a high hemostatic speed, no need for applying pressures to the wound for a long time, safety and non-toxicity, desirable biocompatibility, and no requirement of removal after use. The material is suitable for the application of hemostasis in irregular bleeding such as solid organs in vitro or in vivo and diffuse bleeding.

The invention relates to a method for increasing the sporulation of bacteria such as Bacillus velezensis and Paenibacillus polymyxa using a liquid medium comprising glucose, ammonium sulfate, magnesium chloride and non-ionic surfactant polymer compounds. This method for increasing sporulation is used in a method for generating a large quantity of spores and selecting spores more resistant to higher temperatures than the spores of wild strains. The spores with higher heat resistance than those of the wild strains can be used in a method for eliminating contamination in fermentations.

Colistin is an important last-resort antibiotic, but its production involves significant challenges due to the presence of impurities resulting from various analogs and the limited number of native producers. In response, disclosed are mutant Paenibacillus bacteria that produce Colicin A more efficiently and / or Colicin A that is of higher purity and / or has a cleaner metabolite background. The disclosed mutant bacteria are genetically engineered to inactivate expression of one or more biosynthetic gene clusters (BGCs), increase expression of one or more other BCGs, and / or increase expression of one or more L-DABA pathway genes.

The invention discloses an alkali-resistant xanthan gum side chain lyase mutant and a preparation method thereof, the alkali-resistant xanthan gum side chain lyase mutant is obtained by carrying out site-specific mutagenesis on wild type xanthan gum side chain lyase from paenibacillus nanensis, and the mutation site of the site-specific mutagenesis comprises at least one of Y260V, T387A and I422S. According to the xanthan gum side chain lyase mutant disclosed by the invention, the enzyme activity of the mutant treated by using an alkaline buffer system is obviously improved compared with that of an original enzyme at 40 DEG C, and the enzyme activity is improved to the greatest extent by using the three mutants Y260V / T387A / I422S, so that the xanthan gum side chain lyase mutant is suitable for large-scale degradation of a xanthan gum side chain under an alkaline condition, has important social and economic benefits and is wide in application prospect.

This invention discloses *Paenibacillus alvei* CHWJ-P3 for dissolving phosphorus and potassium in acidic soils and its applications. *Paenibacillus alvei* CHWJ-P3 is classified as *Paenibacillus alvei* CHWJ-P3 and was deposited at the China Center for Type Culture Collection (CCTCC) on December 8, 2025, with accession number CCTCC NO: M 20252813. The *Paenibacillus alvei* CHWJ-P3 of this invention exhibits a significant ability to dissolve phosphorus and potassium in the environment, along with high acid resistance and efficient pH regulation. It can be used for the release of insoluble phosphorus and potassium in soil, i.e., the bioremediation of phosphorus and potassium dissolution in acidic soils; and for the bioremediation of acidic soil pH regulation. It features high remediation efficiency, stable remediation effects, and no secondary pollution, demonstrating promising application prospects.

This invention discloses a *Bacillus subtilis* strain 52-6 with phosphate-solubilizing function and its applications, belonging to the field of microbial technology. This invention provides a *Bacillus subtilis* strain, classified and named *Bacillus subtilis*. Mesobacillus subterraneus Its accession number is CGMCC No. 32719. The colonies of the *Bacillus subtilis* 52-6 are round, light coral red, translucent, with a smooth surface and neat edges. The optimal growth temperature is 35-38℃. It has a short lag phase, grows rapidly, and is easy to cultivate. *Bacillus subtilis* 52-6 can efficiently dissolve insoluble phosphates in soil, promoting the absorption of phosphorus by plants, thus promoting plant growth, effectively improving soil fertility, and reducing the use of chemical fertilizers. *Bacillus subtilis* 52-6 has great potential in promoting plant growth and improving soil fertility.

The application discloses a method for synergistically inducing calcium carbonate deposition and solidification by bacillus subtilis and sphaerotilus paratus. The method comprises the following steps: 1) adding a composite bacterial solution containing bacillus subtilis and sphaerotilus paratus to treat loose particles; and 2) adding a calcification solution to post-treat the treated loose particles. The application also provides bacillus subtilis with a preservation number of CCTCC NO: M 20231896. The composite bacterial solution containing bacillus subtilis and sphaerotilus paratus synergistically promotes the induced calcification efficiency of microorganisms, and improves the cementation performance, toughness and hardness of the calcification material.

The invention belongs to the technical field of microorganisms, discloses a paenibacillus zeae strain and application of the paenibacillus zeae strain in prevention and treatment of plant diseases, and particularly relates to application of the paenibacillus zeae strain in prevention and treatment of lycium barbarum root rot. The paenibacillus zeae is separated from rhizosphere soil of a Chinese wolfberry plant suffering from root rot, the strain number is R2, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36137. The invention further discloses a preparation method of the paenibacillus zeae. The strain can inhibit the growth of various fungal pathogenic bacteria including lycium barbarum root rot, can generate cellulase and amylase, and can induce the expression of defensive genes such as PR1 and CAT2 of plants. Pot experiments show that the control effect of the strain on lycium barbarum root rot reaches 66.66%, the disease morbidity and disease index can be remarkably reduced, and effective biological resources are provided for green control of plant diseases, especially the lycium barbarum root rot.

The present invention relates to a high-efficiency compost-promoting microbial agent, a preparation method, and an application thereof, belonging to the technical field of bio-agriculture and related industries. The microbial agent is composed of the following strains: Bacillus licheniformis DY-1, Bacillus massiliense MS, Bacillus aeruginosa MB, and Paenibacillus aeruginosa XWS-70; the viable cell count of each strain is greater than or equal to 5×10 8 CFU / g; the total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g. Its preparation method includes: strain activation, strain expansion culture, bacterial powder preparation, and strain compounding steps. Its application in rapid composting of organic waste has the following advantages: not only do the four strains not produce antagonism between each other, but they can also significantly accelerate cellulose degradation and improve the degree of composting.

The invention discloses a strain of nitrogen-fixing and acid-producing bacillus strain Paenibacillus sabinae N56, which not only has relatively strong free living nitrogen fixing capacity, but also has the capacity of secreting organic acids (oxalic acid and formic acid), especially has the function of activating inorganic phosphorus (P) in farmland soil, and can be used for preparing a nitrogen-fixing and acid-producing bacillus strain N56, and the nitrogen-fixing and acid-producing bacillus strain N56 can be used for preparing a nitrogen-fixing and acid-producing bacillus strain N56. Meanwhile, growth hormone indoleacetic acid can be secreted, so that the growth of crops is promoted, and the saline-alkaline tolerance is realized. Meanwhile, culture amplification of the nitrogen-fixing paenibacillus N56 is optimized, the problem that the yield of spores of the N56 is low is solved, the number of the spores is increased to 2 billion cfu / ml from 2 billion cfu / ml, the yield of the spores is increased by 10 times, the application potential of the N56 is enhanced, and the production cost can be effectively reduced.

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

The application provides a huperzine A-producing cold-tolerant Paenibacillus sp. strain Sys-Hup-008, the strain is preserved in the China Center for Type Culture Collection, the preservation date is October 31, 2025, and the preservation number is CCTCC NO:M20252403; the 16S rDNA sequence of the strain is shown as SEQ ID NO. 1. The cold-tolerant Paenibacillus sp. strain Sys-Hup-008 provided in the application can metabolically produce huperzine A, can be subjected to large-scale fermentation culture in a short period, and has low fermentation cost, and is not limited by time domain, season and other conditions, so that the problems of high cost, complicated steps and many intermediate pollutants in chemical synthesis of huperzine A can be solved through the microbial fermentation approach. Meanwhile, the application also provides optimized microbial fermentation conditions and a formula of a culture medium, and the yield of huperzine A is high.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a complex microbial inoculant for promoting quality improvement and yield increase of alfalfa as well as a preparation method and application of the complex microbial inoculant. The complex microbial inoculant provided by the invention is prepared from P.aryabhattai 96 and Paenibacillus gramineus H5 according to a specific proportion, and the complex microbial inoculant can assist plants to adapt to a stress environment, increase the biomass and enhance the tolerance and repair ability to pollutants through the mechanisms of nitrogen fixation, phosphorus solubilization and siderophore production.

This invention discloses a Bacillus tergenta Bte2005, with accession number M 2026736. This invention relates to Bacillus terrestris Bte2005, isolated from the hatching system of healthy Litopenaeus vannamei eggs. This strain and its metabolites exhibit strong antagonistic effects against more than ten pathogenic bacteria of marine aquaculture animals, including Vibrio harveyi, Vibrio Erwini, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio anguillarum, Vibrio cholerae, Vibrio shewani, Vibrio rotiferus, Pseudomonas pseudoalteromonas, and luminescent bacteria of mermaids. It demonstrates strong antibacterial activity and a broad antibacterial spectrum. Bacillus terrestris Bte2005 metabolites are abundant and stable. Its ammonium sulfate precipitate extract, hydrochloric acid precipitate methanol extract, ethyl acetate extract, and chloroform extract all exhibit broad-spectrum antibacterial activity. The functional substances that exert its broad-spectrum antagonistic antibacterial effect are complex and diverse, and it does not exert a targeted selection effect on bacteria in the environment, nor does it easily induce tolerance in pathogens. Therefore, it possesses good application safety and ecological safety properties.