39 results about "Paenibacillus validus" patented technology

The invention belongs to the technical field of agriculture, and particularly provides a compound microbial agent and application thereof. The compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02, and the compound microbial agent is prepared from bacillus velezensis RKN1111, bacillus subtilis N-3 and paenibacillus mucilaginosus HX-02. The compound microbial agent can effectively improve the pH and EC of saline alkali soil, increase the content of alkali-hydrolyzable nitrogen, available phosphorus and quick-acting potassium in the soil, and play a significant role in increasing the plant height, stem diameter, chlorophyll content and aboveground biomass of corn plants in coastal saline-alkali soil.

The invention discloses paenibacillus sp. H31, which is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20252669, and the preservation date is November 24, 2025. The invention further discloses a preparation method of the paenibacillus sp. The paenibacillus H31 disclosed by the invention not only has broad-spectrum disease-resistant activity on pathogenic fungi of various plants, but also has a remarkable growth-promoting effect on pepper, so that the paenibacillus H31 can be widely applied to prevention and treatment of agricultural diseases and insect pests, and sustainable development of green agricultural production is promoted. In addition, the paenibacillus H31 also has the effects of hydrolyzing proteinase and cellulose and decomposing ferritin, so that the paenibacillus H31 also can be applied to the scenes of industrial production and the like.

The invention provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae, and a method for efficiently preparing rare ginsenoside F2 by using an enzyme method and a whole-cell method, and particularly provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae and a method for efficiently preparing rare ginsenoside F2 by using a beta-glucosidase mutant from Paenibacillus phyllosphaerae. The method comprises the following steps: engineering-modifying beta-glucosidase by utilizing computer-aided design, constructing a mutant library, carrying out primary screening and secondary screening to obtain two high-activity mutants Q7D and G189A, constructing a combined mutant Q7D / G189A, transforming the combined mutant Q7D / G189A into genetically engineered bacterium escherichia coli for expression, and producing F2 by utilizing a whole-cell method. Finally, the highest yield of 18.01 g / L is achieved after the reaction is carried out for 10 h in a 5 L fermentation tank and is 6.82 times that of a shake flask, and the purity of F2 reaches up to 82.20% after the reaction is carried out for 24 h finally. The invention not only provides an efficient solution for large-scale production of rare saponin F2, but also provides a transferable framework for designing and engineering diversified glycoside hydrolase in customized biological catalysis.

The present invention relates to: a composition and a method for biologically controlling plant or crop diseases, the composition comprising three antagonistic microorganisms such as Bacillus velezensis CT-1, Bacillus amyloliquefaciens PT-1, and Paenibacillus polymyxa BW-1. The germination ability of pathogenic conidia that causes infection by adhering to and inhabiting flowers (floral organs) is suppressed by treating with a culture medium or extract of the novel strain, whereby the proliferation of pathogens is suppressed or the infiltration of bacteria into tissues is blocked, and thus the present invention can be effectively used for the stable production and environmentally friendly quality maintenance of fruits.

The present disclosure provides a Paenibacillus mucilaginosus LT1906 and use thereof, and a microorganism-activated circulating fluidized bed (CFB) fly ash-based cementitious material and a preparation method and use thereof, belonging to the technical field of cementitious materials. The Paenibacillus mucilaginosus LT1906 has a deposit number of CGMCC No. 21337. The strain is capable of dissolving silicate minerals and aluminosilicate minerals in the CFB fly ash to increase dissolution of active SiO2. The strain is used for activating the CFB fly ash-based cementitious material, to ameliorate a large water demand and a low early strength of the cementitious material, thereby improving comprehensive utilization of the CFB fly ash in the field of building materials.

This invention belongs to the field of bio-fermentation technology and relates to a strain of Bacillus videmannii SXZY-01 and its applications, including: isolating and screening a strain of Bacillus videmannii capable of producing tanninase from the surface of tobacco. Bacillus wiedmannii The strain SXZY-01, with accession number CCTCC NO:M 2025151, was used to ferment a biological agent containing SXZY-01. After fermentation optimization, the tannin enzyme activity reached 40.98 U / mL. This invention applies a high-enzyme-activity solution to tobacco leaf fermentation and optimizes the fermentation process, achieving efficient degradation of tannins, significantly improving the sensory quality of tobacco, reducing the bitter aftertaste, and demonstrating outstanding technical effects. It provides a green and environmentally friendly solution for upgrading tobacco quality and is in line with the industry's high-quality development direction.

The present disclosure provides a Paenibacillus strain, and a hemostatic polysaccharide produced by same and use thereof. In the present disclosure, a Paenibacillus sp. 1229 has a deposit number of CCTCC NO: M 2022553. A produced exopolysaccharide (EPS) Hemoadhican has a hemostatic function, and can be prepared as a hemostatic material in different dosage forms for different types of hemostasis. The hemostatic material has a high hemostatic speed, no need for applying pressures to the wound for a long time, safety and non-toxicity, desirable biocompatibility, and no requirement of removal after use. The material is suitable for the application of hemostasis in irregular bleeding such as solid organs in vitro or in vivo and diffuse bleeding.

The invention relates to a method for increasing the sporulation of bacteria such as Bacillus velezensis and Paenibacillus polymyxa using a liquid medium comprising glucose, ammonium sulfate, magnesium chloride and non-ionic surfactant polymer compounds. This method for increasing sporulation is used in a method for generating a large quantity of spores and selecting spores more resistant to higher temperatures than the spores of wild strains. The spores with higher heat resistance than those of the wild strains can be used in a method for eliminating contamination in fermentations.

The invention discloses an alkali-resistant xanthan gum side chain lyase mutant and a preparation method thereof, the alkali-resistant xanthan gum side chain lyase mutant is obtained by carrying out site-specific mutagenesis on wild type xanthan gum side chain lyase from paenibacillus nanensis, and the mutation site of the site-specific mutagenesis comprises at least one of Y260V, T387A and I422S. According to the xanthan gum side chain lyase mutant disclosed by the invention, the enzyme activity of the mutant treated by using an alkaline buffer system is obviously improved compared with that of an original enzyme at 40 DEG C, and the enzyme activity is improved to the greatest extent by using the three mutants Y260V / T387A / I422S, so that the xanthan gum side chain lyase mutant is suitable for large-scale degradation of a xanthan gum side chain under an alkaline condition, has important social and economic benefits and is wide in application prospect.

This invention discloses *Paenibacillus alvei* CHWJ-P3 for dissolving phosphorus and potassium in acidic soils and its applications. *Paenibacillus alvei* CHWJ-P3 is classified as *Paenibacillus alvei* CHWJ-P3 and was deposited at the China Center for Type Culture Collection (CCTCC) on December 8, 2025, with accession number CCTCC NO: M 20252813. The *Paenibacillus alvei* CHWJ-P3 of this invention exhibits a significant ability to dissolve phosphorus and potassium in the environment, along with high acid resistance and efficient pH regulation. It can be used for the release of insoluble phosphorus and potassium in soil, i.e., the bioremediation of phosphorus and potassium dissolution in acidic soils; and for the bioremediation of acidic soil pH regulation. It features high remediation efficiency, stable remediation effects, and no secondary pollution, demonstrating promising application prospects.

This invention discloses a *Bacillus subtilis* strain 52-6 with phosphate-solubilizing function and its applications, belonging to the field of microbial technology. This invention provides a *Bacillus subtilis* strain, classified and named *Bacillus subtilis*. Mesobacillus subterraneus Its accession number is CGMCC No. 32719. The colonies of the *Bacillus subtilis* 52-6 are round, light coral red, translucent, with a smooth surface and neat edges. The optimal growth temperature is 35-38℃. It has a short lag phase, grows rapidly, and is easy to cultivate. *Bacillus subtilis* 52-6 can efficiently dissolve insoluble phosphates in soil, promoting the absorption of phosphorus by plants, thus promoting plant growth, effectively improving soil fertility, and reducing the use of chemical fertilizers. *Bacillus subtilis* 52-6 has great potential in promoting plant growth and improving soil fertility.

The application discloses a method for synergistically inducing calcium carbonate deposition and solidification by bacillus subtilis and sphaerotilus paratus. The method comprises the following steps: 1) adding a composite bacterial solution containing bacillus subtilis and sphaerotilus paratus to treat loose particles; and 2) adding a calcification solution to post-treat the treated loose particles. The application also provides bacillus subtilis with a preservation number of CCTCC NO: M 20231896. The composite bacterial solution containing bacillus subtilis and sphaerotilus paratus synergistically promotes the induced calcification efficiency of microorganisms, and improves the cementation performance, toughness and hardness of the calcification material.

The invention belongs to the technical field of microorganisms, discloses a paenibacillus zeae strain and application of the paenibacillus zeae strain in prevention and treatment of plant diseases, and particularly relates to application of the paenibacillus zeae strain in prevention and treatment of lycium barbarum root rot. The paenibacillus zeae is separated from rhizosphere soil of a Chinese wolfberry plant suffering from root rot, the strain number is R2, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36137. The invention further discloses a preparation method of the paenibacillus zeae. The strain can inhibit the growth of various fungal pathogenic bacteria including lycium barbarum root rot, can generate cellulase and amylase, and can induce the expression of defensive genes such as PR1 and CAT2 of plants. Pot experiments show that the control effect of the strain on lycium barbarum root rot reaches 66.66%, the disease morbidity and disease index can be remarkably reduced, and effective biological resources are provided for green control of plant diseases, especially the lycium barbarum root rot.

The invention discloses a strain of nitrogen-fixing and acid-producing bacillus strain Paenibacillus sabinae N56, which not only has relatively strong free living nitrogen fixing capacity, but also has the capacity of secreting organic acids (oxalic acid and formic acid), especially has the function of activating inorganic phosphorus (P) in farmland soil, and can be used for preparing a nitrogen-fixing and acid-producing bacillus strain N56, and the nitrogen-fixing and acid-producing bacillus strain N56 can be used for preparing a nitrogen-fixing and acid-producing bacillus strain N56. Meanwhile, growth hormone indoleacetic acid can be secreted, so that the growth of crops is promoted, and the saline-alkaline tolerance is realized. Meanwhile, culture amplification of the nitrogen-fixing paenibacillus N56 is optimized, the problem that the yield of spores of the N56 is low is solved, the number of the spores is increased to 2 billion cfu / ml from 2 billion cfu / ml, the yield of the spores is increased by 10 times, the application potential of the N56 is enhanced, and the production cost can be effectively reduced.

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

The application provides a huperzine A-producing cold-tolerant Paenibacillus sp. strain Sys-Hup-008, the strain is preserved in the China Center for Type Culture Collection, the preservation date is October 31, 2025, and the preservation number is CCTCC NO:M20252403; the 16S rDNA sequence of the strain is shown as SEQ ID NO. 1. The cold-tolerant Paenibacillus sp. strain Sys-Hup-008 provided in the application can metabolically produce huperzine A, can be subjected to large-scale fermentation culture in a short period, and has low fermentation cost, and is not limited by time domain, season and other conditions, so that the problems of high cost, complicated steps and many intermediate pollutants in chemical synthesis of huperzine A can be solved through the microbial fermentation approach. Meanwhile, the application also provides optimized microbial fermentation conditions and a formula of a culture medium, and the yield of huperzine A is high.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a complex microbial inoculant for promoting quality improvement and yield increase of alfalfa as well as a preparation method and application of the complex microbial inoculant. The complex microbial inoculant provided by the invention is prepared from P.aryabhattai 96 and Paenibacillus gramineus H5 according to a specific proportion, and the complex microbial inoculant can assist plants to adapt to a stress environment, increase the biomass and enhance the tolerance and repair ability to pollutants through the mechanisms of nitrogen fixation, phosphorus solubilization and siderophore production.

This invention discloses a Bacillus tergenta Bte2005, with accession number M 2026736. This invention relates to Bacillus terrestris Bte2005, isolated from the hatching system of healthy Litopenaeus vannamei eggs. This strain and its metabolites exhibit strong antagonistic effects against more than ten pathogenic bacteria of marine aquaculture animals, including Vibrio harveyi, Vibrio Erwini, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio anguillarum, Vibrio cholerae, Vibrio shewani, Vibrio rotiferus, Pseudomonas pseudoalteromonas, and luminescent bacteria of mermaids. It demonstrates strong antibacterial activity and a broad antibacterial spectrum. Bacillus terrestris Bte2005 metabolites are abundant and stable. Its ammonium sulfate precipitate extract, hydrochloric acid precipitate methanol extract, ethyl acetate extract, and chloroform extract all exhibit broad-spectrum antibacterial activity. The functional substances that exert its broad-spectrum antagonistic antibacterial effect are complex and diverse, and it does not exert a targeted selection effect on bacteria in the environment, nor does it easily induce tolerance in pathogens. Therefore, it possesses good application safety and ecological safety properties.

There is provided a microbial composition comprising Paenibacillus cineris and Micrococcus sp. for enhancing the inoculation rate of T. matsutake mycelium in trees, as well as a use thereof. The microbial composition according to the present invention significantly increases the inoculation rate of T. matsutake mycelium in trees. Furthermore, the composition does not inhibit the growth of T. matsutake mycelium and effectively suppresses harmful microorganisms that interfere with mycelium growth. The microbial composition of the present invention also remarkably improves the survival rate of T. matsutake-inoculated seedlings. According to the method for producing T. matsutake mycelium-inoculated seedlings of the present invention, seedlings with a high inoculation rate of T. matsutake mycelium and a high survival rate can be mass-produced and supplied in large quantities to mushroom cultivators.

The application discloses a feed bacillus NGT8 and application thereof in prevention and treatment of wheat stem base rot. Paenibacillus pabuli The feed bacillus (Bacillus NGT8) provided by the application is preserved in the Guangdong Microbial Culture Collection Center on May 7, 2025, and the preservation number is GDMCC No. 66267. The strain has good bacteriostatic effect on various plant pathogenic bacteria, has broad-spectrum bacteriostatic property, and can be used for preventing and treating plant diseases caused by the pathogenic bacteria. The NGT8 strain has good prevention and treatment effect when applied to the prevention and treatment of wheat stem base rot, and can reduce the incidence and disease index of wheat stem rot. The NGT8 strain provided by the application can be used for developing more plant pathogenic bacteria inhibitors or biocontrol agents, reducing the use of chemical pesticides and pesticide residues, and providing a new way for gradually replacing chemical control with biological control.

The invention provides bacillus mojavensis and application thereof. The bacillus mojavensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO. 35674. The bacillus mojavensis has the advantages that the bacillus mojavensis can be used as a bacillus mojavensis strain; the bacillus mojavensis has multiple biological activities: on one hand, phosphorus-solubilizing related metabolites, potassium-solubilizing related metabolites, siderophores and indoleacetic acid can be generated, and meanwhile, the bacillus mojavensis has the activity of 1-aminocyclopropane-1-carboxylic acid deaminase, so that the growth of seedlings of crops such as jujubes, cucumbers and the like can be remarkably promoted; and on the other hand, the strain has broad-spectrum bacteriostatic activity on 16 pathogenic fungi such as jujube fruit black skin pathogen and cucumber root rot pathogen, has excellent prevention and control effects on jujube black skin disease and cucumber root rot, and can effectively regulate and control a cucumber rhizosphere micro-ecological structure and improve crop disease resistance. Besides, the JZBQ1 strain also has remarkable salt-tolerant and drought-resistant characteristics, can stably play the roles of disease prevention, growth promotion and yield increase under complex cultivation conditions such as conventional cultivated land, saline-alkali soil and drought, and has huge application potential. The invention provides a new strain resource for development and application of an efficient biocontrol inoculant, and has important significance for promoting green development of fruits and vegetables and reducing dependence on chemical pesticides.

The invention discloses bacillus tequilensis SX18 and application of the bacillus tequilensis SX18 in prevention and treatment of radix bupleuri root rot, and belongs to the technical field of microorganisms. The strain is separated from radix bupleuri rhizosphere soil in the Fengzhang area of Baochicken City, Shaanxi Province, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.35247. Experiments show that the strain has a remarkable antagonistic effect on radix bupleuri root rot pathogenic bacteria in a PDA (potato dextrose agar) plate. The invention provides a new microbial resource for green prevention and control of radix bupleuri root rot, can be used for developing environment-friendly biopesticide, and has important significance for guaranteeing safe production of radix bupleuri.

The invention discloses a functional composite microbial inoculant for ecological improvement of moderate and severe saline-alkali soil and a preparation method and application thereof. The composite bacterial agent is prepared by compounding bacterial solutions of paenibacillus polymyxa SMr02, bacillus velezensis DS-R-B-3-5, bacillus laterosporus BHr6-5, lysinibacillus fusiformis SCB19 and pseudomonas japonicus D109 according to the volume ratio of 3: 2: 2: 2: 1. The complex microbial inoculant disclosed by the invention has good saline-alkali tolerance, can obviously improve the stable soil fertility and nutrient content in moderate and severe saline-alkali soil, reduce the soil salinity and alkalinity, relieve saline-alkali stress of salt-tolerant crops and promote growth of the salt-tolerant crops, is simple and convenient to operate, low in cost and environment-friendly, and has a wide application prospect. The method is suitable for ecological improvement of moderate and severe saline-alkali soil in arid areas, irrigation areas in the middle and upstream of the Yellow River and the like, and has good ecological benefits and economic benefits.

The invention relates to the technical field of agricultural microorganisms, and provides a microbial agent for improving soil and application thereof, which can improve soil properties and effectively increase the yield of fruits and vegetables. The microbial agent for improving the soil comprises active bacteria and enzyme, and the mass ratio of the active bacteria to the enzyme is (8-12): (2-4); the active bacteria comprise the following components in percentage by viable count: 28%-32% of bacillus licheniformis, 23%-27% of paenibacillus mucilaginosus, 23%-27% of bacillus amyloliquefaciens and 18%-22% of bacillus subtilis; the sum of the viable count of the four bacteria in the microbial agent is not less than 1 * 10 < 6 > CFU / g; the enzyme comprises protease and lipase, the enzyme activity of the protease is greater than or equal to 50,000 U / g, the enzyme activity of the lipase is greater than or equal to 10,000 U / g, and the weight ratio of the protease to the lipase is (2-3): 1.

The invention relates to the field of microorganisms, and discloses application of bacillus polymyxa and L-gamma-diaminobutyric acid in production of polymyxin B and a method for producing the polymyxin B. According to the method, through the synergistic effect of the bacillus polymyxa and the L-gamma-diaminobutyric acid, the yield of the polymyxin B is remarkably increased, non-target byproducts are reduced, and the large-scale production of the high-purity polymyxin B in medicine and animal husbandry is met.

The application belongs to the technical field of microbial application, and specifically provides a cold-tolerant Paenibacillus polymyxa (Y5R4-9). Peribacillus frigoritolerans Under the condition of saline-alkali stress, inoculation of the strain Y5R4-9 can promote the growth of forage oats in saline-alkali soil by improving the leaf antioxidant enzyme activity and proline content, reducing the MDA content, improving the soil enzyme activity and soil organic matter, increasing the plant height of forage oats, and improving the root morphology, so as to efficiently relieve the stress of saline-alkali on the growth of forage oats.

The invention discloses a composite microbial agent and application thereof in promoting plant growth. The invention discloses a compound microbial agent, which is characterized in that the compound microbial agent comprises Paenibacillus gelatinolyticus PG04 and Lysinibacillus capsici LC43, the compound microbial agent is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation numbers of the compound microbial agent are respectively CCTCC M 2026304 and CCTCC M 2026305. The two strains have excellent salt tolerance. The two components complement each other in function and have synergistic interaction. Pot experiments show that wheat growth can be remarkably promoted by applying the complex microbial inoculant. The microbial agent disclosed by the invention is eco-friendly and controllable in cost, and has a wide application prospect in agricultural utilization.

The application discloses a microbial agent for preventing and treating strawberry root rot and a preparation method thereof. The microbial agent is compounded by Paenibacillus polymyxa and Bacillus pseudomycoides. The Paenibacillus polymyxa has a preservation number of CGMCC No. 25858, and the Bacillus pseudomycoides has a preservation number of CGMCC No. 25859. The preparation method comprises the following steps: after fermentation culture of the two bacteria, the two bacteria are compounded into a wettable powder at a ratio of 1:1, and the wettable powder is applied to the roots by irrigation. The microbial agent prepared by the application can effectively prevent and treat strawberry root rot, promote the growth of strawberry plants, and will not cause drug resistance of pathogenic bacteria, pesticide residues and environmental pollution after use.

The invention discloses an enterobacter strain and application thereof in improving stress resistance of plants. The invention relates to the field of biological agriculture, and provides bacillus fruticola YJD5-23, and the registration number of the bacillus fruticola YJD5-23 in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms is CGMCC No.36069. The bacillus fruticola YJD5-23 has the advantages that the bacillus fruticola YJD5-23 can be used for preparing the bacillus fruticola YJD5-23; the enterobacter sphaeroides YJD5-23 provided by the invention can improve the resistance of arabidopsis thaliana, alfalfa, corn and soybean to saline-alkali stress, specifically, the dry weight, the fresh weight, the plant length, the leaf area and the chlorophyll content are increased, the oxidation resistance is improved, the wilt is reduced, and the physiological and biochemical indexes are improved. The YJD5-23 microbial inoculum improves the property and activity of saline alkali soil and improves the abundance and composition of microbial communities. Detection shows that the YJD5-23 strain has the capabilities of secreting IAA, ACC deaminase and siderophore, dissolving phosphorus, fixing nitrogen and dissolving potassium. The indexes show that the YJD5-23 microbial inoculum has the potential of growth-promoting bacteria and has important application value in the field of agriculture.

This invention provides a cold-resistant *Bacillus subtilis* KY5 and its applications, belonging to the field of agricultural microbiology technology. The cold-resistant *Bacillus subtilis* KY5 provided by this invention is classified and named *Bacillus subtilis*. Peribacillus frigoritolerans The preservation number is CGMCC No. 36309. The cold-resistant *Bacillus subtilis* KY5 provided by this invention was isolated from the epidermis of healthy potato tubers in Heilongjiang Province. It is a local indigenous flora, possessing a natural affinity for the potato host, strong ecological adaptability, and the ability to adapt to cold climates and soil environments. Furthermore, its cultivation conditions are simple, making it easy to produce and apply. It also exhibits significant inhibitory activity against various potato scab pathogens, demonstrating a significant control effect on potato scab. In pot experiment tests, the control effect against potato scab reached 53.69%.