38 results about "Micrococcus" patented technology

This invention belongs to the field of wastewater treatment technology, specifically relating to a method for treating wastewater from industrialized aquaculture. This invention combines physical, biological, and chemical treatment methods, enabling efficient wastewater treatment in a short time, reducing the footprint and operating costs of treatment equipment, and exhibiting high economic efficiency and practicality. The combined use of Spirulina with Bacillus licheniformis, Bacillus subtilis, and Micrococcus luteus forms a stable algae-bacteria symbiotic system in the aquatic environment, accelerating the removal of nitrogen and phosphorus from the aquaculture wastewater and effectively reducing the chemical oxygen demand (COD). After treatment using this method, the wastewater reuse rate is no less than 80%, and the treated wastewater quality meets national discharge standards.

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The invention belongs to the field of new materials for daily use chemicals, and discloses a method for fermenting solanum nigrum, which is characterized in that solanum nigrum roots are fermented by adopting one or more strains of lactic acid bacteria, bifidobacteria, bacillus, micrococcus, saccharomycetes and filamentous fungi to obtain a solanum nigrum fermentation product. The functions of wrinkle resistance, compactness and the like of the withanolaria nipponica fermentation product obtained by fermentation through the method are obviously better than those of unfermented withanolaria nipponica fermentation products and fermentation products taking withanolaria nipponica extracts as fermentation bases. Meanwhile, the invention also provides application of the fermentation product.

The invention discloses an efficient ribosome imprinting sequencing library construction method, and belongs to the technical field of biology. In the enzyme digestion treatment step, a mixed enzyme system of RNase I and micrococcus nuclease is adopted to treat the cell lysis buffer, and the trinucleotide periodicity of RPFs can be remarkably improved to 80% or above. In the target fragment recovery step, the sucrose gradient ultracentrifugation step is omitted, the RPFs are directly recovered by gel electrophoresis, a plurality of enzyme reaction steps in library construction are optimized and integrated, and streptavidin magnetic beads are used for solid phase purification, so that the whole library construction period is greatly shortened from 7 days to 2.5 days. The method does not depend on special equipment such as ultracentrifugation, and can be completed in a conventional molecular biology laboratory. Besides, a multi-enzyme synergistic RNA terminal one-step modification system is innovatively constructed, single-tube one-step completion is realized, the terminal treatment efficiency and consistency are improved, and a key guarantee is provided for obtaining a high-quality and high-complexity Ribo-seq library.

The invention belongs to the technical field of sewage treatment, and particularly relates to an urban domestic sewage treatment method. Chitosan, modified activated carbon and a complex microbial inoculant formed by compounding any two strains of spirulina, rhodotorula mucilaginosa and micrococcus luteus are specifically combined, the chitosan in the composition serves as a carrier of the complex microbial inoculant, nutrients can be provided for survival of the complex microbial inoculant, and the survival time of the microbial inoculant is prolonged; the modified activated carbon has high adsorption capacity on the complex microbial inoculant, so that slow release of the complex microbial inoculant in the later period is facilitated; therefore, the composition provided by the invention can be used for purifying the urban domestic sewage, the purpose of purifying the urban domestic sewage is achieved, ecologicalization and recycling of domestic sewage treatment are realized, and a new thought is provided for urban sewage treatment.

The invention discloses an algae-derived functional active peptide, a preparation and application, and belongs to the technical field of biological active peptides. The amino acid sequence of the algae-derived functional active peptide is at least one of FPRFR and FLF. According to the preparation method, two bioactive peptides with the effects of resisting oxidation and reducing uric acid are obtained through separation and purification by taking micrococcus as a raw material, chemical synthesis is carried out according to an identified peptide fragment sequence, the effects of reducing uric acid and resisting oxidation of a synthesized single peptide fragment and a compounded peptide fragment are determined, and the bioactive peptides have relatively good XOD inhibition rate and DPPH clearance rate. Therefore, the algae-derived functional active peptides not only can be used as natural antioxidants in food, medicines and / or cosmetics, but also can be used as natural uric acid-reducing supplements in medicines; the method has an important application prospect in the development of products with the effects of reducing uric acid and resisting oxidation.

This invention discloses a method for extracting polysaccharides from *Microcystis aeruginosa* and its applications. The method includes fat removal, alcohol-soluble molecule removal, ultrasonic water extraction, flavonoid removal, protein removal, pigment removal, ethanol precipitation, small molecule substance removal, and freeze-drying. This invention combines directional stepwise impurity removal with ultrasonic water extraction to achieve efficient separation of polysaccharides from impurities. The optimal ultrasonic extraction process was determined using response surface methodology: a fixed ultrasonic extraction power of 100W, an ultrasonic extraction temperature of 49℃, an extraction time of 61 min, a solid-liquid ratio of 1:20.5 g / mL, and three extractions. Under these conditions, the polysaccharide yield reached 1.45%. The obtained *Microcystis aeruginosa* polysaccharides exhibit significant antioxidant activity, showing good scavenging effects against DPPH free radicals, hydroxyl free radicals, and ABTS free radicals, with the total reducing power increasing in a dose-dependent manner. This invention provides a simple and controllable extraction process, yielding polysaccharides with high purity and good activity, which can be used as natural antioxidants in functional foods, health products, and cosmetics.

The invention provides a compound fermentation polypeptide composition with whitening, anti-aging, moisturizing and repairing functions and application thereof, and belongs to the technical field of skin care products. The composite fermentation polypeptide composition comprises the following components: a micrococcus lysate, crithmum maritimum callus culture filtrate, a bifidobacterium fermentation product filtrate, a galactose yeast-like bacterium fermentation product filtrate, a tuber melanosporum extract, a peony root extract, acetyl hexapeptide-8, nonapeptide-1, hexapeptide-9, tetrahydromethylpyrimidine carboxylic acid, fibronectin and the like. The composite fermented polypeptide composition not only realizes multi-dimensional mildness and aging resistance, but also has the characteristics of brightening skin, enhancing skin barrier and the like, and is suitable for the field of skin care products.

The invention discloses a method for preparing tobacco extract rich in terpenes by a biological method, which comprises the following steps: (1) grinding and sieving tobacco leaves in the middle of flue-cured tobacco, mixing with deionized water, extracting, filtering and sterilizing to obtain tobacco extract liquid; (2) denucleating bergamot, squeezing to obtain juice, adding tannase for constant-temperature reaction, and heating to inactivate the tannase after the reaction is ended, so as to obtain bergamot extracting solution; (3) respectively culturing micrococcus ZY-02 and klebsiella variicola HNYJ-3, and mixing the seed solutions according to the volume ratio of 1: 1 to obtain an aroma-producing strain seed solution; (4) mixing the tobacco extract with the bergamot extract according to the mass ratio of 100: 1-1: 1, and then mixing the mixture with the aroma-producing fungus seed solution according to the volume ratio of 1: 1000-1: 50 for fermentation; and (5) centrifuging the fermentation liquor, taking supernatant, concentrating, and adding 1, 2-propylene glycol to obtain the tobacco extract rich in terpenes. The terpenoid-rich tobacco extract is prepared by a biological method, so that the quality of the tobacco extract can be effectively improved.

The invention discloses an application of a bupleurum chinense extract in preparation of an antibacterial product. According to the invention, it is found for the first time that various extracts (including aqueous extracts, ethanol extracts and extraction parts of different polar solvents) of bupleurum chinense have relatively good bacteriostatic activity on streptococcus pneumoniae, methicillin-resistant staphylococcus epidermidis, micrococcus luteus and enterococcus faecium. The antibacterial spectrums and effects of different extracts are different, and the research lays a solid foundation for developing novel anti-drug-resistant bacteria drugs and natural-source antibacterial products.

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.

The invention discloses a method for preparing a sweet tobacco extract through biological fermentation and application of the sweet tobacco extract. The method comprises the following steps: preparing tobacco extract liquid, grinding tobacco leaves in the middle of flue-cured tobacco into tobacco powder, sieving, mixing with deionized water in proportion, extracting, filtering and sterilizing; preparing a rosa sterilis extracting solution, squeezing rosa sterilis to obtain juice, adding 100-1000U / mL pectinase, reacting at 45 DEG C for 1-24 hours, and heating to inactivate the pectinase; then culturing micrococcus ZY-02 and gluconacetobacter ZT-01, inoculating a single colony to an LB culture medium, and mixing two seed solutions according to a ratio of 1: 1 after shake cultivation to obtain an aroma-producing bacterium seed solution; and mixing the two leaching solutions according to the ratio of (100: 1)-(1: 1), mixing with the seed solution according to the ratio of (1: 1000)-(1: 50), and carrying out oscillation fermentation at 25-45 DEG C and 50-300r / min for 1-5 days. And centrifuging the fermentation liquor, concentrating the supernate, and adding 1, 2-propylene glycol to obtain the extract. The problems that an existing extract is complex in process or contains many impurities and tobacco smoke stimulates and is dry are solved, the sweetness of cigarettes can be remarkably improved through the extract, stimulation is reduced, and the quality of the extract is superior to that of an extract without gold roxburgh rose or an unfermented extract.

The invention discloses a targeted periprosthetic infection detection, drug delivery and drug intervention system as well as a method and application thereof, and belongs to the field of biological medicine. The detection and drug intervention system comprises a micrococcus nuclease sensitive biological probe, a functional silicon plastid for bearing the micrococcus nuclease sensitive biological probe, light-cured hydrogel and an implant, wherein medicines are carried in the functional silica plastid; a micrococcus nuclease sensitive biological probe is connected to the surface of a functional silicon plastid through electrostatic interaction force to obtain a nuclease probe functional silicon plastid, and the nuclease probe functional silicon plastid is connected to the surface of an implant through photocuring hydrogel. According to the system provided by the invention, the detection is realized, the delivery of the medicine is also realized, and the release of the medicine can also be realized when the occurrence of infection around the prosthesis is judged, so that the intervention treatment of the medicine is carried out.

The anoectochilus formosanus hangover alleviating and liver protecting raw stock is prepared from the following raw materials in parts by weight: 150-350 parts of anoectochilus formosanus raw juice, 100-250 parts of waxberry raw juice, 100-250 parts of Chinese wolfberry fruit raw juice, 10-30 parts of micrococcus oil, 1-5 parts of oriental cherry polyphenol and 1-5 parts of proanthocyanidin. The anoectochilus formosanus raw stock capable of dispelling effects of alcohol and protecting liver is prepared by the following method: 1) weighing anoectochilus formosanus raw juice, waxberry raw juice and Chinese wolfberry fruit raw juice according to the proportion in parts by weight, adding water which is 10-15 times of the total feeding weight, mixing, pulping, sieving and removing dregs to obtain a raw stock base, and the anoectochilus formosanus raw juice, the waxberry raw juice and the Chinese wolfberry fruit raw juice are obtained by directly pulping fresh anoectochilus formosanus raw juice, fresh waxberry fruit and fresh Chinese wolfberry fruit; the invention discloses anoectochilus formosanus protoplasm which comprises the following steps: 1) preparing a protoplasm substrate, 2) transferring the protoplasm substrate into a vacuum homogeneous emulsifying pot, slowly adding micrococcus oil at 38-42 DEG C, and homogenizing to obtain an emulsion, 3) sequentially adding oriental cherry polyphenol and proanthocyanidin under the protection of nitrogen, and homogenizing to obtain protoplasm, and 4) sterilizing the protoplasm, filling with nitrogen, and refrigerating for storage.The anoectochilus formosanus protoplasm has the effects of dispelling the effects of alcohol and protecting the liver.

The invention relates to selective antibacterial application of micrococcus luteus to harmful staphylococcus, a composition containing the micrococcus luteus, or lysates, culture solution or extracts of the micrococcus luteus shows selective antibacterial activity to the harmful staphylococcus, especially staphylococcus aureus, methicillin-resistant staphylococcus aureus (MRSA) and staphylococcus capitis, and the composition can be used for preparing a medicine for treating the harmful staphylococcus, and can be used for preparing a medicine for treating the harmful staphylococcus, especially the staphylococcus aureus, methicillin-resistant staphylococcus aureus (MRSA) and staphylococcus capitis. Therefore, the composition can be used as an antibacterial composition in various ways, such as cosmetics, quasi-drugs, external preparations for the skin, drugs or the like.

There is provided a microbial composition comprising Paenibacillus cineris and Micrococcus sp. for enhancing the inoculation rate of T. matsutake mycelium in trees, as well as a use thereof. The microbial composition according to the present invention significantly increases the inoculation rate of T. matsutake mycelium in trees. Furthermore, the composition does not inhibit the growth of T. matsutake mycelium and effectively suppresses harmful microorganisms that interfere with mycelium growth. The microbial composition of the present invention also remarkably improves the survival rate of T. matsutake-inoculated seedlings. According to the method for producing T. matsutake mycelium-inoculated seedlings of the present invention, seedlings with a high inoculation rate of T. matsutake mycelium and a high survival rate can be mass-produced and supplied in large quantities to mushroom cultivators.

The invention belongs to the field of medicinal chemistry, and discloses an aromatic pleuromutilin derivative with an antibacterial effect as well as a preparation method and application of the aromatic pleuromutilin derivative. The pleuromutilin is structurally modified, aromatic group modification is further increased through a reductive amination method after mercaptoethylamine is introduced into a C-14 side chain, and the structural formula of the pleuromutilin is shown in the specification. Research on an antibacterial mechanism finds that the series of derivatives can effectively interfere with expression of bacterial protein and show unique and excellent antibacterial activity, and the pleuromutilin can be applied to preparation of antibacterial agents. Compared with pleuromutilin in the prior art, most of the pleuromutilin compounds have antibacterial activity on staphylococcus aureus, escherichia coli, salmonella, micrococcus luteus and enterococcus faecalis which are common in in-vitro clinical application, hardly generate drug resistance, have stable safety and are expected to be further optimized into lead compounds of novel antibiotics.

The present invention relates to topical compositions comprising Micrococcus luteus Q24 and a viscosity modifier, the composition having a viscosity of from about 2,500,000 to about 8,000,000 cP at 25°C, and the use of these for improving the appearance of skin or at least one sign of aging, or reducing skin dryness.

The invention discloses application of micrococcus ruber WR1 in drought-resistant cultivation of Chinese chestnuts, and belongs to the technical field of agricultural microorganism application and drought-resistant planting. The application comprises the following steps: (1) carrying out fermentation culture on micrococcus ruber WR1 to obtain WR1 fermentation liquor, and preparing a WR1 microbial inoculum by utilizing the WR1 fermentation liquor; and (2) applying the WR1 microbial inoculum prepared in the step (1) to soil for planting Chinese chestnuts. Based on the characteristics of deep root systems and high tannic acid rhizosphere environments of Chinese chestnuts, a plant endophytic fungus WR1 with a good symbiotic effect with the Chinese chestnuts is obtained through mass screening and is applied to drought-resistant cultivation of the Chinese chestnuts, through symbiosis of the strain and the Chinese chestnuts, nutrient absorption of the Chinese chestnuts can be improved, growth of the Chinese chestnuts is promoted, and the yield of the Chinese chestnuts is increased. By enhancing photosynthetic physiology and an antioxidant defense system, oxidative damage and metabolic disorder under drought stress can be relieved, and the drought resistance of the Chinese chestnuts is improved.

This invention discloses the application of *Micrococcus rubra* WR1 in drought-resistant chestnut cultivation, belonging to the field of agricultural microbial application and drought-resistant planting technology. The application includes the following steps: (1) fermenting and culturing *Micrococcus rubra* WR1 to obtain WR1 fermentation broth, and using the WR1 fermentation broth to prepare WR1 inoculum; (2) applying the WR1 inoculum prepared in step (1) to the soil where chestnuts are planted. Based on the characteristics of chestnut's deep root system and high tannin rhizosphere environment, this invention obtains a plant endophytic fungus WR1 with good symbiotic effect with chestnuts through extensive screening, and applies it to chestnut drought-resistant cultivation. Through symbiosis between this strain and chestnuts, the nutrient absorption of chestnuts can be improved, chestnut growth can be promoted, and the drought resistance of chestnuts can be improved by enhancing photosynthetic physiology and antioxidant defense system, reducing oxidative damage and metabolic disorders under drought stress.

The invention discloses a plant growth regulator for improving crop stress resistance and a preparation method and application thereof, and belongs to the technical field of plant growth regulators. The plant growth regulator disclosed by the invention is prepared from the following raw materials in parts by weight: 10 to 20 parts of betaine, 15 to 25 parts of fulvic acid, 80 to 100 parts of monopotassium phosphate, 3 to 8 parts of amino-oligosaccharin, 50 to 70 parts of compound microorganism fermentation liquor, 5 to 15 parts of radix actinidiae chinensis extract and 160 to 200 parts of water, wherein the compound microorganism fermentation liquor comprises bacillus cereus and brevibacterium paragasteroides. According to the plant growth regulator disclosed by the invention, the Chinese actinidia root extract and the compound microorganism fermentation liquor are innovatively added, so that the stress resistance of the economic crop pepper is remarkably enhanced, the antioxidant enzyme activity of pepper seedlings can be improved, osmotic regulation substances can be increased, and MDA accumulation in bodies is reduced, so that the tolerance of plants to high temperature and other adverse situations is greatly improved; the stability of plants in a severe environment is higher, and the influence of high temperature stress in summer on pepper seedling culture in summer is relieved.

The application discloses a kind of adolescent depression intestinal microorganism marker and its application, belong to the field of biological medicine.The microorganism marker includes one or more of erysipelotrichaceae bacteria, sludge brouds' bacteria, fecal prevo's bacteria, active rumen coccus, hoffmann's true bacillus, pet big anaerobic rod-shaped bacteria and variant rare micrococcus.The application provides a kind of product (such as kit), including detection reagent for detecting the relative abundance of the microorganism marker.The product provided by the application has good feasibility and accuracy, can effectively evaluate whether adolescent is at risk of suffering from depression, provides a new tool for clinical diagnosis.

The application provides a genetically engineered bacterium for producing recovery promoting factor Rpf and application thereof, relates to the technical field of bioengineering, and belongs to prokaryotes. The nucleotide sequence of the genetically engineered bacterium is shown in SEQ ID NO. 1. The sequence is obtained by codon mutation and DNA sequence optimization of a wild-type bacterium recovery promoting factor of Micrococcus luteus in an original nucleotide sequence, and the optimized nucleotide sequence is named Delta Rpf. The original nucleotide sequence is shown in SEQ ID NO. 2. The purity of the Delta Rpf recombinant protein of the application can reach 93 %, which is much higher than the purity of the protein extracted by the prior art. The enzymatic activity of the Delta Rpf recombinant protein prepared by the genetically engineered bacterium constructed by the application is significantly higher than the enzymatic activity of the unmutated Rpf protein.

The invention discloses a skin-beautifying light natural source / biological light filtering composition capable of absorbing and converting ultraviolet light and blue light into visible red light, and preparation and application of the skin-beautifying light natural source / biological light filtering composition. Belongs to the technical field of cosmetics and application. The composition is prepared from the following components in percentage by mass: 2 to 90 percent of bilberry fruit extracting solution, 2 to 90 percent of micrococcus extracting solution, 5 to 90 percent of camellia flower and leaf extracting solution and 0.5 to 6 percent of spirulina extract. Components capable of absorbing and converting ultraviolet light and blue light are found in natural source products and are matched, and a synergistic combination is found; a composite photoprotection system which has efficient light absorption and conversion capability, is natural in source, safe, stable and high in dosage form compatibility is successfully developed, the bottleneck of the current technology is broken through, and full-spectrum precise skin care is achieved.

The application discloses a CdS / C3N4 photocatalytic material and denitrifying bacteria coupling system and a preparation method and application thereof. After sterilization of CdS / C3N4 photocatalytic material, the CdS / C3N4 photocatalytic material is mixed with a denitrifying culture medium to obtain a mixture of the CdS / C3N4 photocatalytic material and the denitrifying culture medium. After centrifugation of LB culture medium containing denitrifying bacteria, wet bacteria bodies are obtained, and the wet bacteria bodies are added into the mixture of the CdS / C3N4 photocatalytic material and the denitrifying culture medium. The coupling system can promote the growth and cell activity of Micrococcus candidus, and the photocatalytic effect of the material reduces the degree of light damage of the bacteria. In addition, the electrons of the photocatalytic material can provide additional electron donors for the bacteria after being excited under light, promote the reactions involving electron transfer in the bacterial metabolism, and are suitable for treatment of wastewater containing nitrate ions.

The invention provides an anti-alcoholism composition and application thereof, the anti-alcoholism composition comprises an anti-alcoholism enzyme preparation and a bioactive preparation, the anti-alcoholism enzyme preparation comprises at least one of ethanol dehydrogenase and acetaldehyde dehydrogenase, the bioactive preparation comprises a product obtained by enzymolysis of protein contained in micrococcus, and the product comprises polypeptide substances. According to the disclosure, the invention can provide the anti-alcohol composition which can effectively relieve the burden caused by the entry of ethanol or metabolites thereof into the liver and has less side effects and the application of the anti-alcohol composition.

The invention discloses a preparation method of a microalgae carrier and application of the microalgae carrier in immobilized enzyme. According to the method, chlorella, nannochloropsis and schizochytrium are taken as objects, and microalgae residues are modified to be prepared into a microalgae carrier by utilizing pretreatment of a deep eutectic solvent in which the ratio of n (ChCl) to n (glycerol) is 1: 2 in combination with an ultrasonic-assisted technology, and the microalgae carrier is used for immobilizing lipase. According to the method, the utilization rate of microalgae can be increased, resource waste and environmental pollution are reduced, a systematic technical path is provided for full-component high-value utilization of microalgae grease and residues, and application and expansion of green enzyme engineering in the fields of food processing and biological manufacturing are expected to be promoted.

Methods for identifying an individual having a predisposition to developing acne, for assessing severity of acne lesions, and for personalized prevention, diagnosis, and treatment of acne are disclosed. The methods include: (a) obtaining microbiome sample from skin of an individual; (b) determining an amount of one or more bacteria in the microbiome sample, wherein the one or more bacteria are selected from Lawsonella, Cutibacterium, Staphylococcus, Corynebacterium, Streptococcus, Micrococcus, and Neisseriaceae; (c) comparing the amount of the one or more bacteria to a control; and (d) identifying a difference between the amount of the one or more bacteria in the microbiome sample and the control.

The present invention belongs to the technical field of genetic engineering. Provided are an antimicrobial protein, and a coding gene and the use thereof. An amino acid sequence of the antimicrobial protein is a sequence as set forth in any one of SEQ ID NOs. 1-12, or a sequence with the same function as the sequence as set forth in any one of SEQ ID NOs. 1-12 after the substitution and / or deletion and / or addition of 1-30 amino acids, or a sequence with the same function as the sequence as set forth in any one of SEQ ID NOs. 1-12 after the addition of 1-20 amino acids to the C-terminus and / or N-terminus thereof. The produced antimicrobial protein provided can hydrolyze bacterial cell walls, enabling lysis of Micrococcus luteus. Upon verification, the antimicrobial protein can significantly inhibit or kill Gram-positive and Gram-negative bacteria and yeast, thereby providing a new antimicrobial protein preparation and a preparation method therefor for industries such as daily chemicals, food, and feeds.