93 results about "Micrococcus" patented technology

The invention provides a fine-grained phytoplankton microscopic image enhancement classification method and a model building method thereof, and belongs to the technical field of underwater image enhancement classification, and the method comprises the following steps: firstly, collecting phytoplankton samples from a plurality of sea areas, and obtaining image data through a microscopic imaging technology; screening, preprocessing and data enhancement are carried out on the images, and a high-quality training set and a high-quality test set are constructed; according to the method, a double-branch deep learning model HySwinFormer is developed, the double-branch deep learning model HySwinFormer comprises a multi-scale context capture branch based on hierarchical vision TransFormer and a local detail enhancement branch designed by adopting a parallel convolution kernel, and joint expression of features is realized through a designed weighted fusion strategy; and training and optimizing the model to obtain a final model. Experimental results show that the classification performance of the HySwinFormer model on multiple data sets is superior to that of an existing mainstream deep learning model, and the classification effect is remarkably improved.

The present disclosure describes a Pseudococcus preparation and a preparation method and application thereof, the Pseudococcus preparation comprises an active ingredient, the active ingredient comprises a specific pro-inflammatory regression mediator (SPMs) and a pro-inflammatory regression mediator (PRMs), the specific proinflammatory regression medium and the proinflammatory regression medium are generated by catalyzing unsaturated fatty acid synthesized by micrococcus by own enzyme. According to the invention, the invention can provide the micrococcus preparation containing the specific proinflammatory regression medium and the proinflammatory regression medium as well as the preparation method and the application of the micrococcus preparation.

This invention belongs to the field of microbial technology, specifically relating to a strain of *Lactobacillus plantarum* that produces antimicrobial peptides and its applications. The *Lactobacillus plantarum* is specifically *Lactobacillus plantarum* (…). Lactobacillus plants Lactobacillus plantarum AF-6, with accession number CGMCC No. 33716, is a product of this invention. It generates a variety of antimicrobial peptides with broad-spectrum antibacterial activity, inhibiting spoilage bacteria such as Escherichia coli, Bacillus subtilis, Salmonella, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus. This provides a more reliable and effective option for food safety preservation.

The utility model provides a novel micro-nano bubble cyclone filter, which comprises a barrel, an upper seal head and a lower seal head, at least one water inlet is tangentially arranged on two sides of the upper end of the barrel, the water inlet is connected with a water inlet pipeline, and a gas injection port is further arranged on the water inlet pipeline; the inner wall of the upper end of the cylinder body is also provided with an oil collecting micro-cavity; an oil discharge outlet is further formed in one side of the upper end of the cylinder body, is connected with the oil collection micro-cavity and is used for discharging oil dirt; the upper sealing head is also provided with an exhaust port; oxygen-free gas injected from the gas injection port is mixed with oily sewage, so that oxygen in water is promoted to be transferred into the gas and is discharged through the exhaust port, and the deoxygenization effect is achieved. The micro-nano bubble cyclone filter disclosed by the utility model integrates a plurality of advanced functions such as cyclone separation, high-efficiency filtration, automatic control, deep deoxygenation and self-adaptive maintenance, so that the overall efficiency of sewage treatment is remarkably improved, the operation cost is reduced, and the micro-nano bubble cyclone filter is an important innovation in the technical field of water treatment.

The utility model provides a novel micro-nano bubble rotational flow filtering system. The novel micro-nano bubble rotational flow filtering system comprises a rotational flow filter, a mixer, a micro-nano bubble generator, a stabilizer and a dissolved air pump, water inlets are tangentially formed in the two sides of the upper end of the barrel body, an oil collecting micro-cavity is further formed in the inner wall of the upper end of the barrel body, and an oil discharging pipe is arranged in the oil collecting micro-cavity and used for discharging oil dirt; and the upper sealing head is also provided with an exhaust port. Light-phase oil stains in sewage can be effectively separated and enriched through the rotational flow air flotation effect, the sump oil recovery efficiency is improved, the micro-nano bubble technology and the dissolved air pump are combined for use, the oil-water separation effect can be enhanced, dissolved oxygen in water can be effectively removed, and favorable conditions are created for follow-up anaerobic treatment or other processes sensitive to oxygen; due to the special property of the micro-nano bubbles, the micro-nano bubbles can be adhered to the surface of the filter element and the interface of the filter element and liquid, the filter element is automatically cleaned through physical action, dirt accumulation is reduced, and the service life and the maintenance period of the filter element are remarkably prolonged.

This invention belongs to the field of wastewater treatment technology, specifically relating to a method for treating wastewater from industrialized aquaculture. This invention combines physical, biological, and chemical treatment methods, enabling efficient wastewater treatment in a short time, reducing the footprint and operating costs of treatment equipment, and exhibiting high economic efficiency and practicality. The combined use of Spirulina with Bacillus licheniformis, Bacillus subtilis, and Micrococcus luteus forms a stable algae-bacteria symbiotic system in the aquatic environment, accelerating the removal of nitrogen and phosphorus from the aquaculture wastewater and effectively reducing the chemical oxygen demand (COD). After treatment using this method, the wastewater reuse rate is no less than 80%, and the treated wastewater quality meets national discharge standards.

The invention provides a biological feed leavening agent for preventing and treating African swine fever as well as a preparation method and application of the biological feed leavening agent, and belongs to the technical field of feeds. The biological feed leavening agent disclosed by the invention is prepared from the following components in parts by weight: 5 to 25 parts of micrococcus luteus, 10 to 50 parts of Weissella mesenteroides SL7, 10 to 50 parts of lactic acid bacteria, 10 to 20 parts of bacillus licheniformis and 40 to 80 parts of clostridium butyricum. The biological feed leavening agent for preventing and treating the African swine fever, provided by the invention, is added into feed, so that the completeness of mucosal barriers of organisms can be maintained, the non-specific immunity of the organisms can be improved, and the threshold value of infectious diseases, especially the threshold value of infection of the African swine fever can be increased.

The present application relates to the field of pharmaceutical chemistry, and specifically discloses a emodin mannich base, a preparation method and application thereof. The emodin mannich base has a structure as shown in formula I, the structure is simple, has strong in-vitro antimicrobial activity, especially high inhibitory activity to staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteus vulgaris, pseudomonas aeruginosa, shigella dysenteriae, salmonella typhimurium and other bacteria, and candida utilis, candida albicans, saccharomyces cerevisiae, monascus and other fungi, and can be used for preparing antibacterial and / or antifungal drugs.

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The application discloses a preparation method of a hawthorn extract for cigarettes and application of the hawthorn extract in cigarettes, and the preparation method comprises the following steps: mixing and sterilizing rutin with water; inoculating Pseudomonas acidovorans ZY-03, Bacillus subtilis SMXP-58, Micrococcus ZY02 and Pichia membranacei into culture media respectively to form seed liquids; inoculating the seed liquids of the Pseudomonas acidovorans ZY-03 and the Bacillus subtilis SMXP-58 into the rutin solution to obtain a fermentation product 1; grinding and crushing hawthorn and mixing the hawthorn with water and heating, then filtering and mixing the hawthorn with the fermentation product 1 to obtain a mixed solution; mixing the four seed liquids and inoculating the mixed solution into the mixed solution; after fermentation, the mixed solution is extracted by refluxing with an organic solvent, centrifuged, rotary evaporated, and added with an organic reagent to obtain the hawthorn extract for cigarettes. The rutin and the hawthorn are mixed and fermented by using microorganisms, the fermentation effect is improved, flavonoids such as rutin and macromolecular substances in the hawthorn are degraded and converted into aroma components, and the quality of the hawthorn extract for cigarettes is improved.

The invention belongs to the field of new materials for daily use chemicals, and discloses a method for fermenting solanum nigrum, which is characterized in that solanum nigrum roots are fermented by adopting one or more strains of lactic acid bacteria, bifidobacteria, bacillus, micrococcus, saccharomycetes and filamentous fungi to obtain a solanum nigrum fermentation product. The functions of wrinkle resistance, compactness and the like of the withanolaria nipponica fermentation product obtained by fermentation through the method are obviously better than those of unfermented withanolaria nipponica fermentation products and fermentation products taking withanolaria nipponica extracts as fermentation bases. Meanwhile, the invention also provides application of the fermentation product.

The invention discloses the application and method of Leuconostoc mesenteroides in the preparation of fermented food or beverage, and relates to the field of microbial fermentation technology. Leuconostoc mesenteroides I1 / 53 produces antibacterial components such as hydrophilic esters, thioaminophenol C, cerulenin and faecalis in fermented sugarcane juice beverage, which can effectively inhibit foodborne pathogens, including Staphylococcus aureus, Escherichia coli, Salmonella, Listeria monocytogenes, Staphylococcus lentus, Bacillus subtilis, Bacillus cereus and Micrococcus luteus; at the same time, it can also enhance the flavor characteristics of sugarcane juice beverage.

The invention discloses an efficient ribosome imprinting sequencing library construction method, and belongs to the technical field of biology. In the enzyme digestion treatment step, a mixed enzyme system of RNase I and micrococcus nuclease is adopted to treat the cell lysis buffer, and the trinucleotide periodicity of RPFs can be remarkably improved to 80% or above. In the target fragment recovery step, the sucrose gradient ultracentrifugation step is omitted, the RPFs are directly recovered by gel electrophoresis, a plurality of enzyme reaction steps in library construction are optimized and integrated, and streptavidin magnetic beads are used for solid phase purification, so that the whole library construction period is greatly shortened from 7 days to 2.5 days. The method does not depend on special equipment such as ultracentrifugation, and can be completed in a conventional molecular biology laboratory. Besides, a multi-enzyme synergistic RNA terminal one-step modification system is innovatively constructed, single-tube one-step completion is realized, the terminal treatment efficiency and consistency are improved, and a key guarantee is provided for obtaining a high-quality and high-complexity Ribo-seq library.

The invention belongs to the technical field of sewage treatment, and particularly relates to an urban domestic sewage treatment method. Chitosan, modified activated carbon and a complex microbial inoculant formed by compounding any two strains of spirulina, rhodotorula mucilaginosa and micrococcus luteus are specifically combined, the chitosan in the composition serves as a carrier of the complex microbial inoculant, nutrients can be provided for survival of the complex microbial inoculant, and the survival time of the microbial inoculant is prolonged; the modified activated carbon has high adsorption capacity on the complex microbial inoculant, so that slow release of the complex microbial inoculant in the later period is facilitated; therefore, the composition provided by the invention can be used for purifying the urban domestic sewage, the purpose of purifying the urban domestic sewage is achieved, ecologicalization and recycling of domestic sewage treatment are realized, and a new thought is provided for urban sewage treatment.

The invention discloses an algae-derived functional active peptide, a preparation and application, and belongs to the technical field of biological active peptides. The amino acid sequence of the algae-derived functional active peptide is at least one of FPRFR and FLF. According to the preparation method, two bioactive peptides with the effects of resisting oxidation and reducing uric acid are obtained through separation and purification by taking micrococcus as a raw material, chemical synthesis is carried out according to an identified peptide fragment sequence, the effects of reducing uric acid and resisting oxidation of a synthesized single peptide fragment and a compounded peptide fragment are determined, and the bioactive peptides have relatively good XOD inhibition rate and DPPH clearance rate. Therefore, the algae-derived functional active peptides not only can be used as natural antioxidants in food, medicines and / or cosmetics, but also can be used as natural uric acid-reducing supplements in medicines; the method has an important application prospect in the development of products with the effects of reducing uric acid and resisting oxidation.

This invention discloses a method for extracting polysaccharides from *Microcystis aeruginosa* and its applications. The method includes fat removal, alcohol-soluble molecule removal, ultrasonic water extraction, flavonoid removal, protein removal, pigment removal, ethanol precipitation, small molecule substance removal, and freeze-drying. This invention combines directional stepwise impurity removal with ultrasonic water extraction to achieve efficient separation of polysaccharides from impurities. The optimal ultrasonic extraction process was determined using response surface methodology: a fixed ultrasonic extraction power of 100W, an ultrasonic extraction temperature of 49℃, an extraction time of 61 min, a solid-liquid ratio of 1:20.5 g / mL, and three extractions. Under these conditions, the polysaccharide yield reached 1.45%. The obtained *Microcystis aeruginosa* polysaccharides exhibit significant antioxidant activity, showing good scavenging effects against DPPH free radicals, hydroxyl free radicals, and ABTS free radicals, with the total reducing power increasing in a dose-dependent manner. This invention provides a simple and controllable extraction process, yielding polysaccharides with high purity and good activity, which can be used as natural antioxidants in functional foods, health products, and cosmetics.

The invention provides a compound fermentation polypeptide composition with whitening, anti-aging, moisturizing and repairing functions and application thereof, and belongs to the technical field of skin care products. The composite fermentation polypeptide composition comprises the following components: a micrococcus lysate, crithmum maritimum callus culture filtrate, a bifidobacterium fermentation product filtrate, a galactose yeast-like bacterium fermentation product filtrate, a tuber melanosporum extract, a peony root extract, acetyl hexapeptide-8, nonapeptide-1, hexapeptide-9, tetrahydromethylpyrimidine carboxylic acid, fibronectin and the like. The composite fermented polypeptide composition not only realizes multi-dimensional mildness and aging resistance, but also has the characteristics of brightening skin, enhancing skin barrier and the like, and is suitable for the field of skin care products.

The present invention relates to the field of biotechnology, and in particular to a Chinese horseshoe crab TaTrs_ALF_α1 antimicrobial peptide and a preparation method and application thereof. The present invention studies a horseshoe crab antimicrobial peptide: TaTrs_ALF_α1 for Chinese horseshoe crabs. It has been verified that the antimicrobial peptide has good inhibitory effects on common pathogenic bacteria in aquaculture: Staphylococcus aureus, Micrococcus luteus, Aeromonas hydrophila and / or Pseudomonas stutzeri. At the same time, it also has a good growth inhibition effect on gastric cancer cells SGC-7901 and / or esophageal cancer cells Eca-109. When used as a drug for treating esophageal cancer, the inhibitory effect on esophageal cancer cells Eca-109 can reach more than 90% when the concentration reaches 80 μg / mL, and the anticancer effect is good. Therefore, the antimicrobial peptide of the present application can be widely used in the fields of drug research and development and animal breeding immunoenhancers, and is a rare antimicrobial peptide.

The invention discloses a method for preparing tobacco extract rich in terpenes by a biological method, which comprises the following steps: (1) grinding and sieving tobacco leaves in the middle of flue-cured tobacco, mixing with deionized water, extracting, filtering and sterilizing to obtain tobacco extract liquid; (2) denucleating bergamot, squeezing to obtain juice, adding tannase for constant-temperature reaction, and heating to inactivate the tannase after the reaction is ended, so as to obtain bergamot extracting solution; (3) respectively culturing micrococcus ZY-02 and klebsiella variicola HNYJ-3, and mixing the seed solutions according to the volume ratio of 1: 1 to obtain an aroma-producing strain seed solution; (4) mixing the tobacco extract with the bergamot extract according to the mass ratio of 100: 1-1: 1, and then mixing the mixture with the aroma-producing fungus seed solution according to the volume ratio of 1: 1000-1: 50 for fermentation; and (5) centrifuging the fermentation liquor, taking supernatant, concentrating, and adding 1, 2-propylene glycol to obtain the tobacco extract rich in terpenes. The terpenoid-rich tobacco extract is prepared by a biological method, so that the quality of the tobacco extract can be effectively improved.

The invention discloses an application of a bupleurum chinense extract in preparation of an antibacterial product. According to the invention, it is found for the first time that various extracts (including aqueous extracts, ethanol extracts and extraction parts of different polar solvents) of bupleurum chinense have relatively good bacteriostatic activity on streptococcus pneumoniae, methicillin-resistant staphylococcus epidermidis, micrococcus luteus and enterococcus faecium. The antibacterial spectrums and effects of different extracts are different, and the research lays a solid foundation for developing novel anti-drug-resistant bacteria drugs and natural-source antibacterial products.

Provided is a method for inactivating viruses or bacteria that has high inactivation effects on enveloped viruses, bacteria of the genus Moraxella and bacteria of the genus Micrococcus even under the condition of using a quaternary ammonium salt-type surfactant at a low concentration,A method for inactivating one or more viruses or bacteria selected from an enveloped virus, a bacterium of the genus Moraxella and a bacterium of the genus Micrococcus and present on a surface of interest, including, bringing a treatment liquid containing (A) a quaternary ammonium salt-type surfactant, (B) an aliphatic alcohol and water into contact with the surface of interest.

This invention discloses the application of *Ulva prostrata* extract in the preparation of preventive and therapeutic antibacterial drugs. The antibacterial components of *Ulva prostrata* are extracted by ethanol extraction using a reflux extraction method. The extract shows significant antibacterial effects against *Staphylococcus aureus*, *Pseudomonas aeruginosa*, *Acinetobacter baumannii*, and *Micrococcus luteus*. The minimum inhibitory concentrations (MICs) are 3.125 mg / mL, 12.5 mg / mL, 6.25 mg / mL, and 3.125 mg / mL, respectively. The extract exhibits good stability to heat treatment and ultraviolet light, and shows good antibacterial effects in neutral and slightly alkaline environments. Metal ions affect the antibacterial effect of the extract, but the diameter of the inhibition zone is greater than 7 mm.

The invention provides a superconducting nanowire single-photon detector film thickness testing structure and method and a preparation method. The film thickness testing structure comprises a substrate, a bottom reflecting layer, a superconducting film layer, a dielectric material layer and a top reflecting layer which are sequentially stacked from bottom to top. Wherein the bottom reflecting layer, the superconducting film layer, the dielectric material layer and the top reflecting layer form an optical microcavity structure; the bottom reflecting layer and the top reflecting layer are used as two reflecting surfaces of the optical microcavity structure; the superconducting film layer and the dielectric material layer are used as spacing layers of the optical microcavity structure. According to the superconducting nanowire single-photon detector film thickness testing structure and method and the preparation method provided by the invention, the problem that the film thickness of the superconducting film layer of the superconducting nanowire single-photon detector cannot be accurately tested by the existing film thickness testing means can be solved.

The present invention relates to a selective antibacterial use of Micrococcus luteus against harmful Staphylococcus sp. This composition comprising Micrococcus luteus, or a lysate, culture medium, or extract thereof exhibits selective antibacterial activity against harmful Staphylococcus sp., particularly Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and Staphylococcus capitis, and thus can be variously used as an antibacterial composition in cosmetics, quasi-drugs, external use skin preparations, medicines or the like.

The invention relates to the technical field of essential oil, in particular to peach leaf essential oil and application thereof. According to the method, the peach leaf essential oil is extracted by adopting a water distillation method, the components of the peach leaf essential oil are identified, 40 volatile chemical components are identified by utilizing headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GCMS), 23 chemical components are identified by utilizing ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS), and the content of the volatile chemical components in the peach leaf essential oil is greatly improved. Researches find that the peach leaf essential oil can effectively inhibit and kill escherichia coli, staphylococcus aureus, micrococcus luteus, pantoea, fusarium oxysporum and monilinia fusarium, and in addition, the invention also finds that the peach leaf essential oil has better oxidation resistance and free radical scavenging ability, so that the invention develops new application of the peach leaf essential oil.

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.

The invention provides a pet anti-aging additive based on compound functional components and a preparation method thereof, and relates to the technical field of pet foods.The additive takes arabinoxylan, micrococcus oil and nano traditional Chinese medicine extracts as matrixes to construct a multi-dimensional anti-inflammatory barrier system, and the multi-dimensional anti-inflammatory barrier system is prepared into the pet anti-aging additive based on the compound functional components. The proanthocyanidins and the micrococcus oil are adopted to form a neuroprotection synergistic interaction system, and meanwhile, a microencapsulation process is combined, so that the bioavailability is improved, and a multi-dimensional anti-aging mechanism of resisting oxidation, regulating and controlling senescence-related markers and resisting inflammation is realized; in-vitro experiments show that the additive can effectively inhibit the expression of SA-beta-gal in canine fibroblasts, and can reduce the MDA content and relieve oxidative damage by improving the activity of SOD and GSH-Px; in-vivo experiments show that the additive can effectively reduce the CCDR score of a test dog, obviously improve the cognitive function of the test dog, improve the NAD + level of plasma, reduce the levels of TNF-alpha, IL-1beta and IL-6 in joint synovial fluid and exert the anti-aging effect, and a safe and efficient new scheme is provided for delaying pet aging.

The invention relates to the technical field of river sewage treatment, and mainly relates to a river sewage treatment fungicide, a treatment method and treatment equipment. The sewage treatment microbial agent comprises the following strains in parts by weight: 10 to 90 parts of bacillus phosphate, 5 to 80 parts of nitrifying bacteria, 5 to 80 parts of denitrifying bacteria, 5 to 60 parts of bacillus, 3 to 65 parts of micrococcus, 3 to 60 parts of lactobacillus plantarum, 3 to 60 parts of halotolerant bacteria, 2 to 50 parts of acetic bacteria, 2 to 50 parts of methanogens, 2 to 30 parts of methanospirillum, 2 to 30 parts of pseudomonas and 2 to 30 parts of activating enzyme and polysaccharide. The sewage treatment fungicide and the treatment method have the effects of high efficiency, low cost, environmental protection and sustainability, the designed treatment equipment can effectively recycle sludge in a settling zone, microorganisms in the sludge can be degraded again conveniently, and sewage treatment is facilitated.

The invention belongs to the technical field of traditional Chinese medicine extracts, and particularly relates to application of a mulberry leaf extract in preparation of antibacterial products. The mulberry leaf extract can be used for resisting bacteria, is particularly suitable for resisting klebsiella pneumoniae, streptococcus pneumoniae, micrococcus luteus, enterococcus faecium, methicillin-resistant staphylococcus epidermidis, sarcina and acinetobacter baumannii, and has great development potential in research of bacteriostatic drugs, bacteriostatic feed additives and other bacteriostatic products in the future.

The present invention relates to a Yunnan micrococcus and its application in passivating heavy metals in livestock and poultry excrement treatment, belonging to the field of heavy metal pollution remediation. The present invention provides Yunnan micrococcus TW001, and a microbial preparation is obtained after strain activation, liquid seed preparation and liquid fermentation. The Yunnan micrococcus TW001 microbial preparation for poultry excrement passivation treatment provided by the present invention is used in soil heavy metal passivation treatment, has the advantages of fast strain growth rate, high fermentation density, strong genetic stability, good compatibility with the environment, and passivation effect on multiple heavy metals such as arsenic, cadmium and mercury, etc., is a good heavy metal biological passivator, and has good application prospects.