292 results about "Micrococcus" patented technology

A Rhodococcus ruber Em (CGMCC No.0868) features that its cells, cell suspension, or immobilized cells can degradate the paraffin and polycyclic arylhydrocarbon because the paraffin and polycyclic arylhydrocarbon are used as its only carbon source and energy source for growing to generate the bioemulsifier of ester. Said emulsifier can obviously decrease the surface tension of aqueous solution, has strong emulsifying power to ester substance, and can increase the solubility of paraffin and polycyclic arylhydrocarbon in water, so it can be used for treating the oil-contained sewage and repairing the petroleum polluted soil.

The invention relates to the field of soil fertilizer and provides a biological organic repairing agent for soil pollution and its application and preparation method. The biological organic repairing agent of the invention is composed of, by weight, 1.5-3 parts of eymogenic bacterium, 1-1.5 parts of wheat bran, 1.5-2 parts of molasses meal, 45-50 parts of ammonium humate, 45-50 parts of hydrolase dregs, 150-200 parts of soybean meal, 100-150 parts of humic acid powder, 80-100 parts of zeolite powder, 25-40 parts of a calcium magnesium phosphate fertilizer, 150-200 parts of cattle and sheep manure, 150-204 parts of plant straw, 25-40 parts of potassium humate, 1.5-3 parts of bacillus mucilaginosus, 1-1.5 parts of Gliocladium roseum, 1.5-2 parts of Streptomycesvinaceus-drappus, 1-1.5 parts of trichoderma spp, 1-1.5 parts of Paecilomyces lilacinus, 1-1.5 parts of bacillus subtilis and 1-1.5 parts of micrococcus. According to the invention, soil pollution control, improvement and nutrition are integrated.

This invention provides ZJB-064(Rhodococcus rubber ZJB-064) of a new microbial strain selected from soil and its application in preparing p-hydroxyben acetamide, in which, said ZJB-064 was preserved in China tipical cultivated subject center in April 14, 2006 with the preservation number of CCTCCNo: M206040 and is used in sybthesizing p-hydroxyben acetamide.

The invention relates to in-situ restoration of pesticide contaminated soil, in particular to a preparation method of microbe immobilized particles for restoring pesticide contaminated soil. Porous carbon is used as a main carrier material, and cellulose, chitosan and sodium alginate are used as auxiliary carriers to realize the purpose of immobilizing the microbes; and the obtained microbe immobilized particles are used for in-situ restoration of the pesticide contaminated soil. The carrier material used in the invention has cheap price, low cost, wide sources, good air permeability and no toxicity to organisms, and can not cause secondary pollution to the soil. The carrier and the immobilizing process thereof can be used for immobilizing multi-bacterium microbes, such as pseudomonas, bacillus, zoogloea, micrococcus and the like, and have certain broad-spectrum applicability; and the bacillus can reach more than 10 billions/gram. Compared with the prior immobilizing method, the invention is characterized by directly sowing the immobilized cell particles into the soil without destroying the original soil structure.

The invention discloses a solid microbial bacterium agent for petroleum degradation. The agent is prepared by mixing of the following components by weight: 85-90 parts of a microbial bacterial fertilizer, and 10-15 parts of a mixed fermentation solution of Micrococcus luteus, Bacillus cereus and Bacillus pumilus. In addition, the invention also provides a preparation method of the solid microbialbacterium agent. The solid microbial bacterium agent provided in the invention can introduce beneficial microorganisms into the soil, optimize the soil microbial system, enhance physical properties of the soil, enhance soil biological activity, and restore hardened and degraded soil, thus being an eco-environment-friendly green bacterium agent. Application of the solid microbial bacterium agent provided in the invention in the soil can promote crop growth, degrade and transform petroleum pollutants in the soil, and also can enhance the stress resistance of crops, as well as improve crop yield.

The invention discloses high-concentration chemical sewage composite inocula. The high-concentration chemical sewage composite inocula comprise, by weight, 180-220 parts of bacillus cereus, 150-160 parts of bacillus circulans, 75-85 parts of Exiguobacterium acetylicum, 90-120 parts of bacillus coagulans, 150-170 parts of pseudomonas aeruginosa, 20-25 parts of micrococcus luteus, 120-140 parts of thiobacillus thiooxidans, 180-230 parts of rhodococci, 20-30 parts of lactobacillus brevis, 50-65 parts of paracoccus denitrificans, 95-112 parts of alcaligenes faecalis, 110-125 parts of pseudomonas putida, 100-130 parts of enterobacter aerogenes, 280-320 parts of beer yeast and 140-160 parts of lactobacillus fermenti. All strains of the composite inocula reasonably cooperate with each other, produce synergistic effects, do not produce antagonism, have a high survival rate, is suitable for one step addition and fast forms dominant bacterial community. The high-concentration chemical sewage composite inocula have unique treatment effects on high-concentration chemical sewage, have a COD clearance rate of 94.17%, reduce an operation cost and promote qualified discharge.

The invention belongs to the field of wastewater treatment and discloses a treatment process applicable to industrial wastewater. The treatment process applicable to the industrial wastewater comprises the following steps: 1) preparing complex microbial inoculant; 2) precipitating wastewater; 3) purifying the wastewater; 4) preparing bacterial manure. The prepared complex microbial inoculant comprises 5-6 parts of micrococcus luteus, 4-5 parts of bacillus mucilaginosus, 3-4 parts of paracoccus denitrificans, 2-3 parts of rhodococcus, 2-3 parts of acidithiobacillus ferrooxidans, 1-2 parts of bacillus megaterium and 1-2 parts of bacillus cereus. The invention also discloses bacterial manure prepared by utilizing the treatment process applicable to the industrial wastewater. The treatment process applicable to the industrial wastewater is simple and feasible, the industrial wastewater can be effectively treated, and the bacterial manure is prepared by utilizing the treatment process applicable to the industrial wastewater.

The invention discloses a method for quickly qualitatively and quantitatively detecting harmful bacteria in beer breweries by applying fluorescence in situ hybridization and fluorescent microcolony combined technology. The method comprises steps of: preparing sample solution and a culture medium; performing fluorescent microcolony and fluorescence in situ hybridization experimental operation; andobserving by using a fluorescence microscope, counting, and qualitatively and quantitatively analyzing the harmful bacteria in the production process of the beer breweries according to fluorescent probes and excited fluorescence with different colors of a carboxyfluorescein diacetate (CFDA) dye. The invention also discloses two specific probes, which are respectively a probe for detecting Lactobacillus brevis, namely 5'-TAGCCGGTGTAACCCTGACTCTG-3' of which one end is marked by Cy3, and a probe for detecting micrococcus tetragenus, namely 5'-GAGATTAGGAAGAACACCAGTGGCGAA-3' of which one end is marked by Cy3. By combining two methods, phenomena that the fluorescence in situ hybridization is difficult to quantitate bacteria and experimental results are easy to show false negative and false positive are avoided, and the problem that the fluorescent microcolony technology cannot qualitatively analyze the bacteria is also solved. Therefore, the requirement on quick detection and qualitative and quantitative requirements for the production of the beer breweries are met.

The present invention relates to a method for resting and evaluating capacity of corrosion prevention system of cosmetic. The invention selects micrococcus pyogenes, bacillus coil, bacillus pyocyaneus, candida albicans, aspergillus niger and salmonella as evaluating strain. The product is grafted into the cosmetic sample solution through the six pathogens and the mixed strains composed of the six pathogens for executing culture experiment. The corrosion prevention capacity of cosmetic is evaluated through comparing and identifying data. The risk resistance capacity of cosmetic for resisting the microbial pollution is effectively evaluated. As the six pathogens have wide representativeness and typical reference value, the six pathogens are used as the typical representative sample strains for evaluating. If the corrosion prevention capacity test is passed, the pollution of product by the microorganism can be effectively prevented, and the possibility of product pollution caused by the reproduction of microorganism can be effectively suppressed. The safety risk caused by product and the cost for frequently executing microbiological examination can be reduced through the basic product formula checked by the corrosion prevention capacity test or all products extended from the basic product formula. The effect is extraordinarily remarkable.

The invention belongs to the technical field of food processing, and particularly relates to safe food preservative. The safe food preservative comprises, by mass, 2-7 parts of gingko leaf extractives, 4-9 parts of lysozyme, 12-15 parts of chitosan, 3-7 parts of spice extractives, 4-10 parts of protamines and 80-100 parts of water. According to the safe food preservative, the main inhibiting objects are pseudomonades, micrococcus, bacilli, enterobacteriaceae, vibrios, halobacterium, mycete and the like, and the application range is wide in beverages, seasoning, cooked wheaten food, cakes, sausages, canned fruit, various melons and fruits, vegetables and the like.

The invention relates to a quinones derivative as well as a preparation method of the quinones derivative and an application of the quinones derivative as an antibacterial agent. During the preparation, marine fungi Nigrospora sp.(HM565952) is subjected to strain culture in a culture medium, then, the marine fungi are subjected to fermentation culture in a fermentation culture medium, next, the obtained fermentation liquid is filtered, thalli are removed, and the fermentation liquid is extracted by ethyl acetate; after the extraction liquid is concentrated, chromatographic separation is carried out, and the obtained eluent is concentrated to obtain two red crystals which are respectively 4-Deoxybostrycin and Bostrycin; and acetyl oxide is added into solution in which the two compounds dissolve for reaction to obtain 3-Acetoxy-4-deoxybostrycin and 3-Acetoxy-bostrycin. The quinones derivative provided by the invention has the obvious antibacterial activity on Bacillus subtilis, Bacillus cereus, Micrococcus tetragenus, Micrococcus lutea, Staphylococcus aureus, Staphylococcus albus, Escherichia coli, Vibrio parahaemolyticus and Vibrio anguillarum and can be used for developing antibacterial agents, in addition, raw materials can be produced in large scales, and the resource limitation is avoided, so the application prospect is wide.

The invention discloses a temperature resistant quaternary ammonium type anion exchange resin phase transfer catalyst and a preparation method thereof, belonging to the synthesis field of anion exchange resin and phase transfer catalyst. The preparation method comprises the following steps: swelling polystyrene alkylated by macroporous chlorine and divinyl benzene copolymer bead body by solvents, adding a certain amount of N-alkyl aminopyridine, stirring and heating, preserving the temperature at 40-100 DEG C, carrying out salifying reaction at the temperature of 40-100 DEG C for 10-30h, filtering after reaction, washing with alcohol and drying to obtain the temperature resistant quaternary ammonium type anion exchange resin phase transfer catalyst, wherein, the amount of substance ratio of micrococcus chlorinus to N-alkyl aminopyridine is 1:1-3, and the amount of substance of micrococcus chlorines is expressed by chlorine element; the solvents used in swelling are as follows: methylene dichloride, tetrahydrofuran, N,N-dimethyl fomamide or n-butyl alcohol; the expressed polystyrene and divinyl benzene co-polymer framework is a repeat unit of the polystyrene and the divinyl benzene; and k, n, and m are respectively 0,1,2,3.

The invention discloses a manufacturing method of a poultry bifidobacteria fermented sausage, comprising the following steps of: selecting poultry meat, mincing and chopping, adding auxiliary materials, uniformly stirring, placing in an environment of 2-5 DEG C, preserving for 16-20 h, optimally selecting lactics, bifidobacteria, staphylococcus and micrococcus, separating, domesticating, culturing, choosing strong bacterial strains, inoculating mixed strains according to 0.3% of the total weight of the poultry meat, mixing stuffing, and carrying out technologies of filling, rinsing, cultivation fermentation, baking and maturation on poultry meat so as to obtain the finished product. The cultivation fermentation time is 2.5-4.5 hours, and the temperature is 35-37 DEG C. The poultry bifidobacteria fermented sausage provided by the invention is rich in nutrients, has a unique flavor, is suitable for the taste of countrymen and is easy to digest. By the adoption of the manufacturing method, shelf-life can be prolonged, conversion from a traditional technology of natural fermentation to a modern technology of pure fermentation is completed, and quality and security are guaranteed.

The invention relates to a histone-modified chromosome co-immunoprecipitation method applicable in a tissue sample, which comprises the following steps: carrying out tissue sample pretreatment; grinding in an ice bath, filtering with sterile gauze, collecting cells in the filtrate, and treating the suspended cells in an enzyme digestion buffer solution under water-bath conditions; adding micrococcus nuclease into the cells with the enzyme digestion buffer solution, carrying out enzyme digestion, and adding an enzyme digestion termination solution with the same amount as the micrococcus nuclease; carrying out high-frequency ultrasonic treatment and centrifugation in an ice bath to obtain a pulverized chromatin solution; taking ProteinA/G, adding a BSA (bovine serum albumin)-containing PBS (phosphate buffer solution), adding an antibody, incubating, adding the pulverized chromatin solution, and incubating over night; and washing with a high salt solution many times on a magnetic rack, eluting DNA (deoxyribonucleic acid) on the magnetic bead with a TE solution, and purifying with a purification solution, extracting the DNA, and dissolving the DNA in ultrapure water. Compared with the prior art, the method can accurately position the combination site of the histone or transcription factor on the high flux level, thereby enhancing the detection effect of the clinic sample.

The invention provides an anthraquinone derivative, as well as a preparation method and application of the anthraquinone derivative serving as an antibacterial agent. The preparation method comprises the following steps: culturing a strain of Nigrospora sp. (HM565952) in a strain culture medium; fermenting the nigrospora sp. in a fermentation culture medium; filtering the fermentation broth, removing thallus, and extracting the fermentation broth with ethyl acetate; concentrating the extract, and performing chromatographic separation, and concentrating the eluent to obtain two types of yellow powder, namely 3,5,8-Trihydroxy-7-methoxy-2-methylanthracene-9,10-dione and austrocortirubin; and adding an acetic anhydride reagent in a solution dissolved with the compound, and reacting to obtain the anthraquinone derivative. The anthraquinone derivative obtained from the Nigrospora sp. (HM565952) has selective inhibitory activity to Bacillus subtilis, Bacillus cereus, Micrococcus tetragenus, Micrococcus lutea, Escherichia coli, Staphylococcus aureus, Staphylococcus albus, Vibrio parahaemolyticus and Vibrio anguillarum, and can be used for developing selective bacteriostatic agents; and the raw materials can be produced on a large scale, and not limited by resources. Therefore, the anthraquinone derivative has the advantage of wide application prospect.

The invention discloses a fusion protein for Micrococcus pyogenes surface protein, and also discloses the method for preparing the same as well as its usage. The fusion protein is fused by Micrococcus pyogenes fnb and thioredoxin Trx on expression carrier, and the process comprises preparation of Micrococcus pyogenes surface protein fnb coding gene, colony of fnb coding gene into expression carrier and protein fusion. The fusion protein is soluble protein and retains immunity of natural protein. It is detected that said fusion protein is characterized by good response for humoral immunity and cell immunity, enlargement of protection range of protein vaccine and prevention of infection caused by most of Micrococcus pyogenes.

Recalcitrant chemical oxygen demand (COD) of a liquid is reduced in a water treatment system. The method includes pretreating the liquid in a pretreatment unit to remove indigenous bacteria or microbes to a population level below which the indigenous organisms can interfere with the screened and externally introduced microorganisms. The liquid is then provided to a reactor that has a filter bed formed with a carrier material. Special microbes are screened and used to colonize the carrier material to remove recalcitrant COD. A biofilm is cultured on the surface of the carrier material to immobilize the screened microbes in the reactor. The method further includes percolating the liquid from the pretreatment unit through the filter bed colonized with the screened microbes to degrade at least part of the recalcitrant COD under aerobic conditions. In one embodiment, the filter is formed with biological granular activated carbon (GAC) as the carrier material and the screened microbes comprise at least one microbial species selected from the group consisting of Bacillus, Comamonas, Arthrobacter, Micrococcus, Pseudomonas, Pediococcus, Achromobacter, Flavobacterium, Mycobacterium, Rhodanobacter, Stenotrophomonas and Yeast.

The invention discloses a low salt pickling and artificial fermentation process of Xuanwei ham, the technical proposal includes four steps of raw material selection, low salt pickling, fermentation fungi group cultivation and artificial fermentation, and a fermentation fungi group comprises Penicillium, lactic acid bacteria, Bacterium lacticum and Micrococcus in the ratio of 2.7:0.73:0.2:1. The advantages are that after raw material meat is inoculated with the lactic acid bacteria, the lactic acid bacteria uses carbohydrates such as glucose for fermentation to produce lactic acid, gelatinous tissues are formed by degeneration of muscle proteins under acidic conditions, bonding force among meat blocks is increased, and hardness and elasticity of meat products are improved, the lactic acid bacteria has high salt tolerance, can be used for fermentation of sugar to produce the lactic acid to play antibacterial and hypoglycemic effects and improve meat freshness; the Micrococcus has effects of decomposition of proteins and fat and reduction of nitrates, large amounts of amino acids are produced after the proteins are broken down, the amino acids are sources of a fresh taste, meanwhile by reduction of the cancerogenic substance nitrates, health of food customers can be protected.

The invention provides a method for preparing high-quality biological organic fertilizer through municipal sewage sludge. The method includes the steps of raw material preparing, fermented material preparing, sterilization, anaerobic fermentation, fermentation promoter injection, aerobic fermentation and finished product preparing. Anaerobic fermentation agents used in the anaerobic fermentation step contain methane thermophilic micrococcocci, lactobacillus planetarium, metallophilic thermoanaerobacter ethanolicus and white high-temperature actinomycetes. Aerobic fermentation agents used in the aerobic fermentation step contain trichoderma reese, lactobacillus delbruckii and vibrio proteolyticus. According to the prepared bio-organic fertilizer, the number of fecal coliform bacteria ranges from 23 to 26 per g, the death rate of ova of roundworm ranges from 99.2% to 99.8%, and the number of effective living bacteria ranges from 440 millions CPF/g to 52 millions CPF/g.

The invention discloses a composite microbial inoculant for treating grease wastewater, and application and a use method thereof. The composite microbial inoculant is compounded from the following components in percentage by volume: 10-30% of Pseudomonas cepacia, 10-30% of micrococcus, 10-20% of Bacillus licheniformis, 10-20% of Bacillus amyloliquefaciens, 10-20% of Pexpansum and 10-20% of Yarrowia lipolytica. The OD600 of each strain is 0.30+/-0.05. Under the action of the composite microbial inoculant for treating grease wastewater, the ecological niche in the wastewater system is more abundant, different types of microbes closely cooperate to perform more efficient metabolism, thereby enhancing the wastewater treatment efficiency and lowering the treatment cost. The composite microbial inoculant is simple in manufacturing technique and use mode, has obvious enhancement actions on treatment effect of the high-concentration grease wastewater, and has very high industrialization application value.