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Solid microbial bacterium agent for petroleum degradation and preparation method thereof

A technology of microbial inoculum and microbial fertilizer, which is applied in the field of solid microbial inoculum for degrading petroleum and its preparation, can solve the problems of microorganisms contacting and ingesting pollutants, difficulty in ingesting pollutants by microorganisms, and difficulty in migration of exogenous microorganisms.

Inactive Publication Date: 2012-12-26
CHANGAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most abiotic factors can be regulated by engineering measures, such as adjusting soil water content, pH, adding organic carbon sources and inorganic nutrients, etc.; Difficulty in the migration of microorganisms, resulting in the inability of microorganisms to effectively contact and ingest pollutants. Difficulty in the uptake of pollutants by microorganisms is a bottleneck in in-situ bioremediation

Method used

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  • Solid microbial bacterium agent for petroleum degradation and preparation method thereof
  • Solid microbial bacterium agent for petroleum degradation and preparation method thereof
  • Solid microbial bacterium agent for petroleum degradation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The solid microbial bacterial agent used to degrade petroleum in this example is made by mixing the following components: 90g of microbial fertilizer, Micrococcus luteus 4006, preservation number CGMCC No.6072), Bacillus cereus 4033, preservation number CGMCC No.6074) and Bacillus pumilus 4070, preservation number CGMCC No.6073) mixed fermentation broth 10g; the water content of the solid microbial agent is 5wt%; the mixed fermentation broth The content of Micrococcus luteus was 1.0×10 8 A / mL~1.0×10 9 individual / mL, the content of Bacillus cereus was 1.0×10 7 A / mL~1.0×10 10 individual / mL, the content of Bacillus pumilus was 1.0×10 9 A / mL~1.0×10 11 pcs / mL; the microbial fertilizer is 12-bacteria microbial fertilizer produced by Xi'an Delong Biotechnology Co., Ltd., the number of effective viable bacteria in the bacterial fertilizer is ≥0.8 billion / g, and the organic matter content is not less than 25%. The mass content of acid is not less than 20%, and the total mas...

Embodiment 2

[0068] The solid microbial bacterial agent used to degrade petroleum in this embodiment is made by mixing the following components: 85 kg of microbial fertilizer, Micrococcus luteus 4006, preservation number CGMCC No.6072), Bacillus cereus 4033, preservation number CGMCC No.6074) and Bacillus pumilus 4070, preservation number CGMCC No.6073) mixed fermentation broth 15kg; the water content of the solid microbial agent is 15wt%; the mixed fermentation broth The content of Micrococcus luteus was 1.0×10 8 A / mL~1.0×10 9 individual / mL, the content of Bacillus cereus was 1.0×10 7 A / mL~1.0×10 10 individual / mL, the content of Bacillus pumilus was 1.0×10 9 A / mL~1.0×10 11 pcs / mL; the microbial fertilizer is 12-bacteria microbial fertilizer produced by Xi'an Delong Biotechnology Co., Ltd., the number of effective viable bacteria in the bacterial fertilizer is ≥0.8 billion / g, and the organic matter content is not less than 25%. The mass content of acid is not less than 20%, and the to...

Embodiment 3

[0076] The solid microbial bacterial agent used to degrade petroleum in this example is made by mixing the following ingredients: 89g of microbial fertilizer, Micrococcus luteus 4006, preservation number CGMCC No.6072), Bacillus cereus 4033, preservation number CGMCC No.6074) and Bacillus pumilus 4070, preservation number CGMCC No.6073) mixed fermentation broth 11g; the water content of the solid microbial agent is 9.5wt%; the mixed fermentation broth The content of Micrococcus luteus in 1.0×10 8 A / mL~1.0×10 9 individual / mL, the content of Bacillus cereus was 1.0×10 7 A / mL~1.0×10 10 individual / mL, the content of Bacillus pumilus was 1.0×10 9 A / mL~1.0×10 11 pcs / mL; the microbial fertilizer is 12-bacteria microbial fertilizer produced by Xi'an Delong Biotechnology Co., Ltd., the number of effective viable bacteria in the bacterial fertilizer is ≥0.8 billion / g, and the organic matter content is not less than 25%. The mass content of acid is not less than 20%, and the total m...

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Abstract

The invention discloses a solid microbial bacterium agent for petroleum degradation. The agent is prepared by mixing of the following components by weight: 85-90 parts of a microbial bacterial fertilizer, and 10-15 parts of a mixed fermentation solution of Micrococcus luteus, Bacillus cereus and Bacillus pumilus. In addition, the invention also provides a preparation method of the solid microbialbacterium agent. The solid microbial bacterium agent provided in the invention can introduce beneficial microorganisms into the soil, optimize the soil microbial system, enhance physical properties of the soil, enhance soil biological activity, and restore hardened and degraded soil, thus being an eco-environment-friendly green bacterium agent. Application of the solid microbial bacterium agent provided in the invention in the soil can promote crop growth, degrade and transform petroleum pollutants in the soil, and also can enhance the stress resistance of crops, as well as improve crop yield.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to a solid microbial bacterial agent for degrading petroleum and a preparation method thereof. Background technique [0002] The hazards of petroleum pollutants are mainly manifested in the hazards and impacts on humans and animals, soil and natural water bodies. After oil is discharged into the soil, it will affect the permeability of the soil. After the soil particles are polluted by oil, they are not easy to be infiltrated by water, and cannot form an effective water conduction channel in the soil. The water seepage decreases, and the water permeability decreases. Petroleum hydrocarbons, most of which are high-molecular organic matter, stick to the root system of plants to form a layer of mucous membrane, which hinders the respiration and water absorption of the root system, and even causes the root system to rot. In addition, petroleum is rich in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08B09C1/10C12R1/265C12R1/085C12R1/07
Inventor 王文科申圆圆李春荣王周峰韩晓娇廖鹏辉王兆琪
Owner CHANGAN UNIV
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