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Solid microbial bacterium agent for petroleum degradation and preparation method thereof

A technology for microbial inoculum and microbial fertilizer, which is applied in the field of solid microbial inoculum for degrading petroleum and its preparation, can solve the problems of difficulty in ingesting pollutants by microorganisms, difficulty in migrating exogenous microorganisms, and contacting and ingesting pollutants by microorganisms.

Inactive Publication Date: 2013-10-16
CHANGAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most abiotic factors can be regulated by engineering measures, such as adjusting soil water content, pH, adding organic carbon sources and inorganic nutrients, etc.; Difficulty in the migration of microorganisms, resulting in the inability of microorganisms to effectively contact and ingest pollutants. Difficulty in the uptake of pollutants by microorganisms is a bottleneck in in-situ bioremediation

Method used

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  • Solid microbial bacterium agent for petroleum degradation and preparation method thereof
  • Solid microbial bacterium agent for petroleum degradation and preparation method thereof
  • Solid microbial bacterium agent for petroleum degradation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The solid microbial inoculant for degrading petroleum in this example is prepared by mixing the following components: 90 g of microbial fertilizer, Micrococcus luteus 4006 (CGMCC No. 6072), Bacillus cereus (Bacillus cereus) 4033, preservation number CGMCC No.6074) and Bacillus pumilus (Bacillus pumilus 4070, preservation number CGMCC No.6073) mixed fermentation broth 10g; the water content of the solid microbial inoculum is 5wt%; the mixed fermentation broth The content of Micrococcus luteus is 1.0×10 8 pcs / mL~1.0×10 9 pcs / mL, the content of Bacillus cereus is 1.0×10 7 pcs / mL~1.0×10 10 pcs / mL, the content of Bacillus pumilus is 1.0×10 9 pcs / mL~1.0×10 11 The microbial fertilizer is a 12-bacteria microbial fertilizer produced by Xi'an Delong Biotechnology Co., Ltd., the number of effective viable bacteria in the bacterial fertilizer is ≥080 million / g, the organic matter content is not less than 25%, and the humic The mass content of acid is not less than 20%, and th...

Embodiment 2

[0068] The solid microbial inoculant for degrading petroleum in this example is prepared by mixing the following components: 85kg of microbial fertilizer, Micrococcus luteus 4006 (CGMCC No.6072), Bacillus cereus (Bacillus cereus) 4033, preservation number CGMCC No. 6074) and Bacillus pumilus (Bacillus pumilus 4070, preservation number CGMCC No. 6073) mixed fermentation broth 15kg; the water content of the solid microbial inoculum is 15wt%; the mixed fermentation broth The content of Micrococcus luteus is 1.0×10 8 pcs / mL~1.0×10 9 pcs / mL, the content of Bacillus cereus is 1.0×10 7 pcs / mL~1.0×10 10 pcs / mL, the content of Bacillus pumilus is 1.0×10 9 pcs / mL~1.0×10 11 The microbial fertilizer is a 12-bacteria microbial fertilizer produced by Xi'an Delong Biotechnology Co., Ltd., the number of effective viable bacteria in the bacterial fertilizer is ≥080 million / g, the organic matter content is not less than 25%, and the humic The mass content of acid is not less than 20%, and...

Embodiment 3

[0076] The solid microbial inoculant for degrading petroleum in this example is prepared by mixing the following components: 89 g of microbial fertilizer, Micrococcus luteus 4006 (Deposit No. CGMCC No. 6072), Bacillus cereus (Bacillus cereus) 4033, preservation number CGMCC No.6074) and Bacillus pumilus (Bacillus pumilus 4070, preservation number CGMCC No.6073) mixed fermentation broth 11g; the water content of the solid microbial inoculum is 9.5wt%; the mixed fermentation broth The content of Micrococcus luteus in 1.0×10 8 pcs / mL~1.0×10 9 pcs / mL, the content of Bacillus cereus is 1.0×10 7 pcs / mL~1.0×10 10 pcs / mL, the content of Bacillus pumilus is 1.0×10 9 pcs / mL~1.0×10 11 The microbial fertilizer is a 12-bacteria microbial fertilizer produced by Xi'an Delong Biotechnology Co., Ltd., the number of effective viable bacteria in the bacterial fertilizer is ≥080 million / g, the organic matter content is not less than 25%, and the humic The mass content of acid is not less th...

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Abstract

The invention discloses a solid microbial bacterium agent for petroleum degradation. The agent is prepared by mixing of the following components by weight: 85-90 parts of a microbial bacterial fertilizer, and 10-15 parts of a mixed fermentation solution of Micrococcus luteus, Bacillus cereus and Bacillus pumilus. In addition, the invention also provides a preparation method of the solid microbial bacterium agent. The solid microbial bacterium agent provided in the invention can introduce beneficial microorganisms into the soil, optimize the soil microbial system, enhance physical properties of the soil, enhance soil biological activity, and restore hardened and degraded soil, thus being an eco-environment-friendly green bacterium agent. Application of the solid microbial bacterium agent provided in the invention in the soil can promote crop growth, degrade and transform petroleum pollutants in the soil, and also can enhance the stress resistance of crops, as well as improve crop yield.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculants, in particular to a solid microbial inoculum for degrading petroleum and a preparation method thereof. Background technique [0002] The harm of petroleum pollutants is mainly manifested in the harm and impact on human beings, animals, soil and natural water bodies. After the oil is discharged into the soil, it will affect the permeability of the soil. The soil particles are not easily infiltrated by water after being polluted by the oil, and an effective water conduction channel in the soil cannot be formed. Petroleum hydrocarbons, most of which are macromolecular organics, adhere to the root system of plants to form a layer of mucous membrane, hinder the respiration of the root system and the absorption of water, and even cause root rot. In addition, oil is rich in reactive groups, which combine with inorganic nitrogen and phosphorus, and inhibit nitrification and dephosphorylation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08B09C1/10C12R1/265C12R1/085C12R1/07
Inventor 王文科申圆圆李春荣王周峰韩晓娇廖鹏辉王兆琪
Owner CHANGAN UNIV
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