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Micrococcus luteus and application thereof

A technology of Micrococcus luteus and microorganisms, applied in the field of bioengineering, can solve the problems of slow degradation and long duration of petroleum hydrocarbon pollutants, achieve the effects of optimizing soil microbial system, improving physical properties, and promoting degradation

Inactive Publication Date: 2013-07-31
CHANGAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The microbial remediation technology of oil-contaminated soil has been greatly developed in foreign countries in the past ten years, but the degradation rate of petroleum hydrocarbon pollutants is slow, resulting in a long duration of the treatment process, which has always been a prominent difficulty in this technology, so looking for High-efficiency petroleum-degrading bacteria are currently a research hotspot in microbial remediation of petroleum-contaminated soils

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  • Micrococcus luteus and application thereof
  • Micrococcus luteus and application thereof

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Embodiment Construction

[0028] Screening of strains:

[0029] Step 1. Enrichment: The soil samples collected from the soil oil pollution 10000mg / kg restoration area of ​​the red clover rhizosphere restoration area about 10cm away from the Shaanxi Provincial Groundwater and Ecological Environment Engineering Research Center Test Site in Weiyang District, Chang'an University, Weishui Campus, Xi'an City were sterile Weigh 10.0g, put it into 100.0mL petroleum / inorganic salt liquid medium, and place it in a constant temperature shaker at 28±1℃, 130r / min for enrichment culture for 7 days; In the inorganic salt liquid medium, enrichment and domestication were carried out 3 times, each time for 7 days; the composition of the petroleum / inorganic salt liquid medium was: NaNO 3 0.15%, (NH 4 ) SO 4 0.15%, K 2 HPO 4 0.1%, MgSO 4 ·7H 2 O0.05%, KCl0.05%, FeSO 4 ·7H 2 O0.001%, CaCl 2 0.0002%, distilled water 1000mL, crude oil (Yanchang Petroleum Dehydrated Crude Oil) 0.5%, the above are mass percentages, me...

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Abstract

The invention discloses a micrococcus luteus 4006 with a collection number of CGMCC No.6072 (China General Microbiological Culture Collection Center Number 6072). In addition, the invention further discloses application of the micrococcus luteus in degradation of petroleum pollutants. The micrococcus luteus originates from soil, so that the micrococcus luteus quickly becomes a dominant populationand takes up a certain ecological niche after entering the soil, so as to effectively promote degradation of petroleum hydrocarbons, optimize soil microorganism systems, improve soil physical characteristics, improve soil biological activity and repair hardened and degraded soil. The micrococcus luteus is a dominant strain screened by taking raw oil as an unique carbon source, can change the property of the raw oil in the growth and propagation process to change the components of the raw oil so as to take effects of emulsifying, wetting and dispersing the raw oil, and can degrade the petroleum pollutants in the soil in a short time.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a micrococcus luteus and its application. Background technique [0002] The microbial remediation technology of oil-contaminated soil has been greatly developed in foreign countries in the past ten years, but the degradation rate of petroleum hydrocarbon pollutants is slow, resulting in a long duration of the treatment process, which has always been a prominent difficulty in this technology, so looking for High-efficiency petroleum-degrading bacteria are currently a research hotspot in the microbial remediation technology of petroleum-contaminated soil. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a Micrococcus luteus that can be used to degrade petroleum pollutants in view of the above-mentioned deficiencies in the prior art. This strain is a dominant strain selected from crude oil as the only ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/265
Inventor 申圆圆王文科李春荣王周峰
Owner CHANGAN UNIV
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