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A kind of bacterial strain jn5 for degrading petroleum hydrocarbons in oily sludge and its application

A technology for petroleum hydrocarbons and bacterial strains, applied in the field of bioremediation, can solve the problems of no investment in large-scale applications, cumbersome cultivation process operations, and large environmental factors, so as to avoid complex reaction products, optimize soil microbial systems, and reduce complex steps. Effect

Active Publication Date: 2020-08-04
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using metabolic surfactant microorganisms to treat oily sludge can increase the solubility of petroleum hydrocarbons, which can improve the degradation efficiency of petroleum hydrocarbons, and use biological treatment to remove harmful substances such as PAHs. Biological treatment can simultaneously degrade aliphatic hydrocarbons and aromatics in oily sludge However, the biological treatment method has limitations such as being greatly affected by environmental factors and a long treatment period, so the treatment technology for biological treatment of oily sludge has not yet been put into large-scale application in China
At the same time, the current microbial degradation agents are mainly compound microbial agents. Various strains need to be cultured and activated separately and then mixed in a certain proportion. cumbersome operation
In the prior art, there is no technical solution for using a single bacterial strain to achieve relatively excellent degradation of petroleum hydrocarbons in oily sludge

Method used

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  • A kind of bacterial strain jn5 for degrading petroleum hydrocarbons in oily sludge and its application
  • A kind of bacterial strain jn5 for degrading petroleum hydrocarbons in oily sludge and its application
  • A kind of bacterial strain jn5 for degrading petroleum hydrocarbons in oily sludge and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Degradation and remediation test of oily sludge in Liaohe Oilfield

[0060] Take oily sludge from Liaohe Oilfield, air-dry and grind, pass through a 1mm sieve, and set aside. Pick a single colony in the sterilized LB liquid medium, seal it with an air-permeable sealing film, and place the Erlenmeyer flask in a shaker at 120rpm and 30°C for 24h activation. Weigh 40g of the above-mentioned oily sludge after air-drying, grinding and sieving, add Pseudomonas stutzeri JN5 activated bacterial liquid with OD600=1 at an inoculum of 10%, adjust the water-soil ratio to 2:5 with distilled water, and seal with a breathable sealing film. Place all Erlenmeyer flasks in a constant temperature incubator at 30°C for static culture, take samples every 5 days, and use infrared spectrophotometer to determine the content of total petroleum hydrocarbons in oily sludge. The degradation curve is as attached image 3 Shown. It can be seen from the figure that the bacteria has a good de...

Embodiment 2

[0064] Example 2: Degradation effect of Pseudomonas stutzeri JN5 on petroleum hydrocarbons in oily sludge under different pH values

[0065] Take oily sludge from Liaohe Oilfield, air-dry and grind, and pass through a 1mm sieve. Weigh 40g of the above-mentioned oily sludge in a conical flask, and use dilute H 2 SO 4 Adjust the pH of the soil to 1, 3, 5, 7, 9, 11 with NaOH, add Pseudomonas stutzeri JN5 activated bacterial solution with OD600=1 at 10% of the inoculum, and adjust the water-soil ratio to 2:5 with distilled water. Breathable sealing film for sealing. All the conical flasks were placed in a constant temperature incubator at 30°C for static cultivation and samples were taken 25 days later, and the total petroleum hydrocarbon content in the oily sludge was measured by an infrared spectrometer. The degradation effect of strain Pseudomonas stutzeri JN5 on petroleum hydrocarbons in oily sludge under different pH conditions is as follows: Figure 4 Shown. by Figure 4 It c...

Embodiment 3

[0066] Example 3: Degradation effect of Pseudomonas stutzeri JN5 on petroleum hydrocarbons in oily sludge at different temperatures

[0067] Take oily sludge from Liaohe Oilfield, air-dry and grind, and pass through a 1mm sieve. Weigh 40g of the above-mentioned oily sludge into an Erlenmeyer flask, add Pseudomonas stutzeri JN5 activated bacterial liquid with OD600=1 at an inoculum of 10%, adjust the water-soil ratio to 2:5 with distilled water, and seal with a breathable sealing film. Place the Erlenmeyer flasks in a constant temperature incubator at 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, and 50°C for static culture, and take samples 25 days later, using infrared The spectrophotometer measures the total petroleum hydrocarbon content in oily sludge. The degradation effect of strain Pseudomonas stutzeri JN5 on total petroleum hydrocarbons in oily sludge at different temperatures is as follows Figure 5 Shown. by Figure 5 It can be seen that the strain of the present i...

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Abstract

The invention discloses a high-efficiency degrading bacterium JN5 for petroleum hydrocarbons in oily sludge and its application, belonging to the technical field of bioremediation. The degrading bacterium JN5 is preserved in the China General Microorganism Culture Collection Management Center, the preservation number is CGMCC NO.14976, and the classification number is Pseudomonas stutzeri. The use of the bacterial strain JN5 having the function of degrading petroleum hydrocarbons in the present invention is to treat oily sludge by degrading petroleum hydrocarbons. The degradation rate of the bacteria to total petroleum hydrocarbon (TPH) in oily sludge was 73.73% within 30 days. The bacterium acts as a single strain against n‑C 10 ~n‑C 26 The straight-chain petroleum hydrocarbons have strong degradation ability. The optimal degradation conditions for the bacteria to petroleum pollutants are 20-50°C and pH 7-11. The biological method is used in the remediation technology of oily sludge, and has the advantages of high efficiency, low cost, and environmental friendliness.

Description

Technical field [0001] The invention relates to the technical field of bioremediation, in particular to a strain capable of degrading and repairing oily sludge, and its application in degrading oily sludge. Background technique [0002] Since the 20th century, petroleum has become one of the most important energy sources for mankind. While being exploited and used in large quantities, the harm caused by petroleum and its processed products to the environment and humans is becoming more and more serious. Since my country’s reform and opening up, the annual output of crude oil has exceeded 100 million tons. China has become one of the top ten oil-producing countries in the world. China has more than 400 oil and gas fields for exploration and development, which are distributed in 25 of the country, accounting for about 3% of the country’s land area. Provinces (cities) and autonomous regions. However, in the process of rapid economic and technological development and rising oil deman...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/38C02F101/32C02F103/10
CPCC02F11/02C02F2101/32C02F2103/10C12N1/20C12N1/205C12R2001/38
Inventor 叶正芳姜楠赵泉林
Owner PEKING UNIV
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