376 results about "Petroleum Pollution" patented technology

This invention discloses a solid microbial agent for degrading petroleum pollutants and petroleum products. The solid microbial agent is prepared by: mixing primary seed solutions of Bacillus subtilis and Sphingobacterium multivorum at a volume ratio of (0.5-20):1, fermenting to obtain mixed bacterial fermentation solution, and mixing the mixed bacterial fermentation solution and turfy soil at a weight ratio of (0.1-1):1. The active living bacteria number of Bacillus subtilis is 106-1010/mL, and that of Sphingobacterium multivorum is 107-1012/mL. The solid microbial agent has such advantages as high activity in soil, rapid growth and no secondary pollution to environment, and can be used for biological bioremediation of petroleum polluted soil and petroleum product leakage fields.

A Rhodococcus ruber Em (CGMCC No.0868) features that its cells, cell suspension, or immobilized cells can degradate the paraffin and polycyclic arylhydrocarbon because the paraffin and polycyclic arylhydrocarbon are used as its only carbon source and energy source for growing to generate the bioemulsifier of ester. Said emulsifier can obviously decrease the surface tension of aqueous solution, has strong emulsifying power to ester substance, and can increase the solubility of paraffin and polycyclic arylhydrocarbon in water, so it can be used for treating the oil-contained sewage and repairing the petroleum polluted soil.

The invention relates to a microorganism composite bacterial agent applicable to the restoration of saline alkali soil polluted by petroleum, and a preparation method thereof, and belongs to the technical field of environment protection. The bacterial agent mainly comprises the following 3 components: composite microorganism bacterial liquid, nutrients, and a surfactant. The composite microorganism bacterial liquid is obtained by performing liquid culture of 3 petroleum hydrocarbon degrading bacteria of bacillus megaterium P9 strains (with a preservation number of CGMCC No. 4270), pseudomonas sp. P4 strains (with a preservation number of CGMCC No. 4269), and achromobacter xylosoxidans P2 strains (with a preservation number of CGMCC No. 4268) to obtain microorganism bacterial strains, and mixing the strains with the nutrients. The bacterial strains are preserved at China microorganism strain preservation management committee general microbiological center. The composite microorganism bacterial liquid, the nutrients and the surfactant are mixed according to a ratio of 30-200:1-2:0.2-0.5 so as to obtain the microorganism composite bacterial agent. The microorganism composite bacterial agent prepared by the invention has high removing efficiency for petroleum pollution in saline alkali soil, can both reduce the pH value of the saline alkali soil, and improve soil physical and chemical properties.

The invention belongs to the technical field of bioremediation of marine oil pollution, and relates to an immobilized algal-bacterial symbiotic system for processing petroleum pollutants. The immobilized algal-bacterial symbiotic system is prepared by the following steps: (1) selection and axenic cultivation of algae species, firstly carrying out lysozyme and sodium dodecyl sulfate (SDS) sterile pretreatment on chlorella algae fluid, and then inoculating into a BG11 culture solution containing antibiotics to carry out sterile cultivation, so as to obtain sterile chlorella algae fluid for later use; (2) selection and cultivation of petroleum degrading bacteria; (3) preparation of composite carrier bacteria algae gel balls, namely immobilizing the sterile chlorella algae fluid obtained in the step (1) and the petroleum degrading bacteria obtained in the step (2) by adopting sodium alga acid and polyvinyl acetate (PVA) as immobilized carriers, and finally obtaining the immobilized algal-bacterial symbiotic system. The immobilized algal-bacterial symbiotic system can float on the water to carry out degradation of petroleum pollution, and is free of secondary pollution, free of toxic action on microorganisms, rapid in reaction speed, stable and reliable in operation, less in microorganism loss, and free of separation.

A method of using indigenous bacteria to restore oil-contaminated soil is to add mixed indigenous bacteria screened and enriched from oil-contaminated soil to oil-contaminated soil for bioremediation, and the specific steps include: oil-contaminated soil Grinding and sieving pretreatment, using inorganic salt medium to screen and enrich indigenous bacteria from oil-contaminated soil, adding enriched indigenous bacteria to oil-contaminated soil, and adding a certain proportion of inorganic nutrients to oil-contaminated soil Compost after stirring evenly, and plow and water the treated soil regularly. The method can be used for both in-situ remediation and ex-situ remediation of oil-contaminated soil, and can be used for remediation of high-concentration petroleum-contaminated soil and low-concentration petroleum-contaminated soil, and the medicines used are easy to obtain and easy to operate. And the degradation efficiency is high, and will not cause secondary pollution.

The invention belongs to the technical field of environmental treatment and in particular relates to a microorganism-electric power two-segment repairing method for petroleum contaminated soils. According to the microorganism-electric power two-segment repairing method disclosed by the invention, conversion between two segments including microorganism repairing and electrokinetic repairing is carried out with the ratio of the total petroleum hydrocarbon content and the total organic carbon content in contaminated soils, and the purpose of preparing the petroleum contaminated soils is achieved; microorganisms are capable of preferentially degrading light components which are easily degraded in petroleum hydrocarbon and then removing heavy components by virtue of the strengthening effect of an electric field, and staged degradation of pollutants is realized; the problem that the pollutants can not be completely degraded by virtue of single microorganisms is solved, and the energy consumption by virtue of single electrokinetic remediation is also lowered; the microorganism-electric power two-segment repairing method is rapid in speed for repairing the petroleum contaminated soils, high in efficiency and low in cost; and compared with single microorganism repairing and single electrokinetic repairing, the efficiency is improved by above 20-40 percent.

The invention relates to a method for preparing a hydrophobic lipophilic cotton fabric material through structuring a zinc oxide fine structure. The method comprises the following steps of: combining a sol-gel method with a hydrothermal method, i.e. uniformly laying prepared zinc oxide nano seed crystals on the surface of a cotton fabric through utilizing the sol-gel method at first, then growing zinc oxide nanorods toward fixed directions on the surface of the cotton fabric through utilizing the hydrothermal method, finally, modifying the cotton fabric through utilizing long-chain fatty acid-palmitic acid so as to have superhydrophobicity, and wrapping cotton with the cotton fabric for oil absorption. According to the method, the specific structure of zinc oxide is utilized to structure the superhydrophobic cotton fabric surface, and moreover, the cotton fabric has lipophilicity, good oil absorption after wrapping the cotton and a good practical application value in petroleum pollution water body remediation.

The invention relates to Pseudomonas sp. LD23, and the strain preservation number is CGMCC No,11679. The invention further relates to application of the Pseudomonas sp. LD23 in petroleum degradation and preparation of sodium alginate-activated carbon immobilized microspheres containing the Pseudomonas sp. LD23. The Pseudomonas sp. LD23 is a strain separated and screened from soil which is serious in petroleum pollution, can keep the high petroleum degradation rate under the low temperature condition and lays a foundation for petroleum-polluted soil treatment in future; meanwhile, the biological carbon immobilization conditions are optimized, the degrading effect of the immobilized petroleum degrading bacterium to raw petroleum is initially inspected, and a scientific basis is provided for remedying petroleum oil through the biological carbon immobilized microspheres.

The invention relates to a preparation method of petroleum-degrading bacteria immobilized embedded particles. The preparation method comprises the following steps: (1) mixing 35.8wt%-46.5wt% of water hyacinth, 14.2wt%-18.5wt% of wheat straws and 35wt%-50wt% of walnut shell-based activated carbon to obtain a mixture as an auxiliary carrier; (2) inoculating 3.0wt%-7.0wt% of the auxiliary carrier onto 10wt%-20wt% of a petroleum-degrading bacteria seed liquid and adsorbing; (3) dissolving 8.0wt%-10wt% of polyvinyl alcohol in 80-100 DEG C water, adding 1.2wt%-1.5wt% of sodium alginate, uniformly mixing, cooling and adding the balance of water; and (4) uniformly mixing the substances obtained in the step (2) and (3) to constitute an embedded system, dropwise adding the embedded system into a CaCl2 solution and crosslinking to form balls, transferring the balls into a Na2SO4 solution and immobilizing to obtain the immobilized embedded particles. The petroleum-degrading bacteria immobilized embedded particles are applied in in-situ remediation of petroleum-polluted soil to achieve good remediation effect on the petroleum-polluted soil.

The present invention relates to a sponge material with a polyurethane sponge surface modified by constructing a zinc oxide fine structure, and a preparation method thereof. According to the preparation method, a sol-gel method and a hydrothermal method are combined to use, wherein prepared zinc oxide nanometer crystal seeds are uniformly paved on the polyurethane sponge surface through the sol-gel method; then zinc oxide nano-rods grow on the surface in a directional manner through the hydrothermal synthesis method; a long-chain fatty acid-palmitic acid is adopted to modify so as to achieve super-hydrophobicity; and finally the hydrophobic effect and the oil absorption capacity of the modified sponge are determined. According to the present invention, the special morphology structure of zinc oxide is adopted to construct the super-hydrophobic sponge surface, and the super oil absorption capacity of the sponge surface is not affected, such that the sponge material has potential application value for petroleum-contaminated water body repair.

The invention discloses a health evaluation method for a river mouth and bay ecological system. The method comprises the following steps of establishing a river mouth and bay ecological environment evaluation index system, determining an ecological environment evaluation index and weights of evaluation factors thereof, detecting and collecting the data of each evaluation factor of the ecological environment evaluation index and computing an index evaluation value, computing a water environment ecological index evaluation value of each station, computing a sea area evaluation value, and computing a health index of the river mouth and bay ecological system. An evaluation result obtained by using the evaluation method of the invention can basically reflect the pressure on an offshore area ecological system caused by relatively large artificial disturbances, including discharge to the sea, artificial breeding and petroleum pollutions, meanwhile can also reflect main pollution risk factors, and also plays a certain forewarning role with respect to potential pollutant factors, thus having a certain reference value and significance for reference, and being capable of providing scientific bases and guidance information to future ecological protection and management of the river mouth and bay ecological system.

The invention provides Rhodococcusruber with the collection number of CGMCC (China General Microbiological Culture Collection Center) No.4795, a microbial agent comprising Gordonia, and application of the Gordonia or the microbial agent in degradation of petroleum hydrocarbon, bioremediation of petroleum pollution, bioremediation of petroleum polluted soil, and bioremediation of petroleum polluted water. The Rhodococcusruber with the collection number of CGMCC No.4795 and the microbial agent comprising the same have high capacity of degrading the petroleum hydrocarbon, and can be widely applied to degradation of the petroleum hydrocarbon and remediation of the petroleum pollution, such as remediation of the petroleum polluted soil, remediation of the petroleum polluted water and the like.

The invention provides a preparation method and an implementation technology for a petroleum-contaminated soil remediation agent system, and relates to a preparation method and an application method for a soil remediation agent. The soil remediation agent system is composed of soil conditioning liquid, a petroleum hydrocarbon degrading bacterium agent, a solid waste carrier, a biological polymer bacterial agent and a nutrient solution. The implementation technology comprises the operation steps that the soil conditioning liquid is sprayed firstly, then the petroleum hydrocarbon degrading bacterium agent is sprayed, an oxygen increasing agent is spread, then the biological polymer bacterial agent is sprayed, solid fertilizer carrier particles are spread, and finally the biological polymer bacterial agent is sprayed again. According to the preparation method and the implementation technology for the petroleum-contaminated soil remediation agent system, the petroleum-contaminated soil remediation agent system can improve the dissolution effect of petroleum and soil, promote the growth of petroleum hydrocarbon microorganisms and inhibit the reproduction of harmful microorganisms; for 5% or less of the petroleum-contaminated soil, the petroleum degradation rate reaches 39-43.7% under the condition that the temperature is 5-10DEG C; and the petroleum degradation rate reaches 59.8% under the condition that the temperature is 15-20 DEG C, and the petroleum degradation rate reaches more than 87.5% under the condition that the temperature is 25-30DEG C.

The present invention discloses an ozone-permeable reactive barrier repair system and a method for groundwater repair by using the system, and belongs to the technical field of groundwater pollution repair. According to the present invention, based on a characteristic of difficult repair of petroleum polluted groundwater, groundwater of a petroleum polluted groundwater pollution region is subjected to ozone reinforcement aeration, and a permeable reactive barrier is installed below water flow of the groundwater. With the present invention, groundwater polluted by petroleum and organic matterscan be effectively repaired while volatile components in groundwater and soil can be effectively treated; characteristics of strong adaptability to water quality of petroleum polluted groundwater, high stability, easy operation, simple treatment process, and continuous operation are provided; water quality of the treated water is good; only adsorbent replacement according to a prompt is required;and characteristics of economy, saving and high treatment efficiency are further provided.

The invention provides an arthrobacter strain highly effectively degrading phenanthrene, and an application thereof. The phenanthrene-degrading arthrobacter strain has a preservation number of CGMCC No. 6581. The strain provided by the invention can effectively degrade polycyclic aromatic hydrocarbon and heterocyclic substances in soil and oil sludge, and can be effectively applied in biodegradation treatments of petroleum-contaminated soil and PAHs pollutants in soil in loess highlands. Especially a phenanthrene degradation rate can be higher than 98%. The invention also provides a formula of a nutrient solution for the growth and degradation of the strain, and a method for applying the strain in petroleum-contaminated soil field reparation.

Reversibly switchable negatively charged surfactants are provided. The anionic surfactant is formed in aqueous solution by providing an absence of CO₂ and is converted to a non-surfactant neutral state through exposure to CO₂. The anionic switchable surfactants are useful to stabilize emulsions. In the neutral state they are useful to separate immiscible liquids. The surfactants find uses in polymerization, the oil industry, remediation of oil-contaminated soil and recapture of oil contaminant, and formation of solid particles such as plastics and nanoparticles.

The invention relates to a preparation method for an embedding floating bacteria agent of nutritive salt, expandable graphite and microorganism together. The preparation method for the embedding floating bacteria agent of the nutritive salt, the expandable graphite and the microorganism together comprises the steps of using a sodium alginate solution as an embedding frame, adding a nutritive salt solution, the expandable graphite and petroleum degrading bacteria to the sodium alginate solution, mixing evenly, dripping into a calcium chloride solution to embed, crosslinking for the night under the condition of 4 DEG C, and obtaining an embedding bacteria agent capable of strengthening bioremediation effect. The bacteria agent prepared by the preparation method for the embedding floating bacteria agent of the nutritive salt, the expandable graphite and the microorganism together is suitable for in-situ remediation of marine petroleum pollution, and has the advantages of being long in floating time, good in mass transfer performance, high in petroleum degrading efficiency, and the like. The method that the nutritive salt and bacteria solutions are embedded together is adopted by the embedding bacteria agent, defects that nutrition of marine environment is poor and additionally the nutritive salt is diluted quickly are overcome, and floating of the bacteria agent is achieved by addition of the expandable graphite. When the embedded bacteria agent is used, the embedded bacteria agent can be directly added to the surface of a polluted water body, effective contact with petroleum pollutant is ensured, biodegradation efficiency is effectively improved, and recycling is facilitated, and risk of a secondary pollution does not exist.

The invention discloses a highly effective degradation strain JN6 for petroleum hydrocarbons in oily sludge and application thereof, which belong to the technical field of bioremediation. The degradation strain JN6 is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO. 14977, and the classification number is Comamonas sp. The strain JN6 with a petroleum hydrocarbon degradation function which is disclosed by the invention is applied to treatment of oily sludge by degrading petroleum hydrocarbons, the degradation rate of the single strain of bacteria on total petroleum hydrocarbon (TPH) in oily sludge is 62.16 percent within 30 days, and the optimum degradation conditions of the bacteria on petroleum pollutants are 15 DEGC to 40 DEGC and the pH value of 7 to 11. The biological method is used in oily sludge remediation technology, and has the advantages of high effectiveness, low cost, environment-friendliness and the like.