65 results about "Petroleum Pollution" patented technology

The invention discloses a flora prediction and petroleum pollution remediation method based on machine learning. The method comprises the following steps: collecting multiple groups of experimental data of a diesel oil pollution sample treated by a microbial agent, extracting environmental factors, microbial community characteristics and target response variables to construct a training data set after missing value processing, abnormal value detection and standardized pretreatment, and importing the data set into a preset machine learning model to obtain a training result; carrying out feature learning, classification training and hyper-parameter optimization by adopting a GridSearchCV method in combination with 10-fold cross validation; evaluating the correlation between a target response variable classification result and the features through a multivariable Pearson's correlation matrix, and constructing an optimal test set; and finally, selecting an optimal prediction model according to a preset index. According to the method, the model training quality is improved through data preprocessing and correlation analysis, efficient flora prediction and algorithm application evaluation are achieved by means of multiple machine learning algorithms, scientific support is provided for petroleum pollution remediation, and remediation accuracy and efficiency are improved.

The invention discloses a Gordonia ovorans YDY-4 strain and an application of the Gordonia ovorans YDY-4 strain in degradation of n-hexadecane. The cholesterol-eating Gordonia sp. YDY-4 is named as the cholesterol-eating Gordonia sp. YDY-4, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC M 20251290, and the cholesterol-eating Gordonia sp. YDY-4 is named as the cholesterol-eating Gordonia sp. YDY-4 and is preserved in the China Center for Type Culture Collection (CCTCC). The Gordonia ovorans YDY-4 provided by the invention has the capability of degrading n-hexadecane under the condition that the pH (Potential of Hydrogen) is 7.0 to 9.0, and can grow at 28 to 32 DEG C. The Gordonia ovorans YDY-4 provided by the invention has relatively strong environmental adaptability and n-hexadecane degradation capability, and provides a basis for bioremediation of petroleum polluted environment and application of a microbial enhanced treatment technology.

The invention is applicable to the technical field of industrial wastewater treatment, and provides a construction method of a microalgae-in-situ bacteria strengthening system for degrading naphthenic acid, which comprises the following steps: separating and identifying two strains of in-situ bacteria from a discharge port of an oil-producing wastewater treatment plant, and constructing a three-strain typical stain-resistant green alga-in-situ bacteria co-culture system. The strengthening system can highly tolerate and efficiently degrade naphthenic acid, can effectively remove nitrogen and phosphorus, and has higher biomass and high-value compound yield. The microalgae-in-situ bacterium strengthening system can reduce pollutant discharge and improve the microalgae resource utilization rate, and the microalgae-in-situ bacterium strengthening system can be used as a biological strengthening degradation technology of naphthenic acid and can be suitable for purification treatment of oil production wastewater and petroleum polluted water areas.

The invention belongs to the technical field of solid waste recycling, and discloses efficient carbon sequestration pseudomonas stutzeri from an oil-containing solid waste residue source and application of the efficient carbon sequestration pseudomonas stutzeri, and the efficient carbon sequestration pseudomonas stutzeri is pseudomonas stutzeri A58-5 with the preservation number of CCTCC NO: M 20241582. The Pseudomonas stutzeri is obtained by taking Pseudomonas stutzeri A58 separated from oil-containing solid waste residues of an oil field as an original strain and carrying out ARTP mutagenesis and screening, has the excellent characteristics of reducing pollution and sequestering carbon and is relatively strong in stress resistance; the method has wide application prospects in the fields of deep recycling (soil conversion) of oil-containing solid waste residues, improvement of the carbon sequestration capacity of the petroleum-contaminated soil, remediation of the petroleum-contaminated soil and the like, and is of great significance in pollution reduction and sequestration increase in the petroleum and petrochemical industry under the background of supporting double carbon.

The application discloses a floating composite microbial preparation for removing marine oil pollution and a preparation method thereof. The floating composite microbial preparation contains bacillus thuringiensis NH26 strain, hollow glass microbeads and a lipopeptide surfactant purified from a fermentation liquor of the NH26 strain, has floating characteristics, can rapidly activate and efficiently degrade oil hydrocarbon, has good degradation capacity for alkanes and polycyclic aromatic hydrocarbons in seawater, and still has good degradation capacity for alkanes in seawater containing high-concentration oil hydrocarbon. The floating composite microbial preparation can be used for removing marine oil pollution and repairing marine environment. In addition, the application further provides a preparation method of the floating composite microbial preparation. The preparation method has simple preparation process, the obtained floating composite microbial preparation has uniform particle size, has high embedding rates for nitrogen and phosphorus, and can be prepared on a large scale.

This invention discloses a method for preparing heavy metal adsorbent materials (PCS) by co-milling and pyrolysis of petroleum-contaminated soil and iron oxides. First, iron oxides or their natural minerals are added to the petroleum-contaminated soil. Then, a high-energy ball milling process is used for mechanochemical activation. The milled mixture is then subjected to slow pyrolysis to form carbonized soil with a surface rich in carbonates and metal oxides. This carbonized soil can be used to adsorb heavy metals from wastewater, with a maximum adsorption capacity of over 300 mg / g for lead. This method simultaneously achieves efficient remediation and high-value resource utilization of PCS through the exploitation, value-added transformation, and "waste-to-waste" approach of the endogenous resources of petroleum-contaminated soil. It contributes to the innovation of petroleum-contaminated soil remediation models and the synergistic remediation of soil-water pollution.

The invention discloses trichoderma longibrachiatum for degrading petroleum hydrocarbon in a saline-alkali environment, a culture and application of the trichoderma longibrachiatum, and relates to the technical field of microorganisms. The preservation number of the trichoderma longibrachiatum is CGMCC (China General Microbiological Culture Collection Center) No. The trichoderma longibrachiatum provided by the invention has an efficient petroleum degradation characteristic, provides a practical and effective solution for the petroleum pollution problem of the saline-alkali soil, can efficiently degrade petroleum hydrocarbon in the saline-alkali soil, and has a good application prospect.

The invention discloses a Bosea thioxidans DY-5 strain and an application of the Bosea thioxidans DY-5 strain in degradation of phenanthrene and benzopyrene. The strain Bosea thioxidans DY-5 is obtained through domestication, separation and identification from petroleum-contaminated soil of Ningbo, phenanthrene and benzopyrene can be used as carbon sources, and after the strain is cultured in an inorganic salt culture solution with the initial concentration of phenanthrene and benzopyrene being 50 mg.L <-1 > and 25 mg.L <-1 > respectively for 7 days, the degradation rate of phenanthrene and benzopyrene can reach 75% or above. Therefore, the strain has good application potential in the aspect of bioremediation of polycyclic aromatic hydrocarbon.

This invention discloses a benzo[a]pyrene-degrading fungus, *Nigrospora osmanthi* DL-7, for use in petroleum-contaminated soil, along with its inoculum and applications. This invention domesticated, isolated, and identified a benzo[a]pyrene-degrading strain, *Nigrospora osmanthi* DL-7, from petroleum-contaminated soil in Ningbo. DL-7 can utilize benzo[a]pyrene as a carbon source, even at an initial benzo[a]pyrene concentration of 25 mg·L⁻¹. ‑1 After culturing in an inorganic salt broth for 7 days, the pure DL-7 strain achieved a degradation rate of over 85% for benzo[a]pyrene. When prepared as a bacterial agent, the degradation rate of benzo[a]pyrene could be increased to over 90%. Therefore, this strain and its bacterial agent have good application potential in the bioremediation of polycyclic aromatic hydrocarbons.

The invention belongs to the technical field of oil reservoir microorganisms, and particularly relates to a zoococcus strain and application thereof. The bacterial strain is a Planococcus sp. Bacterial strain WL-9, and is a new strain of the Planococcus sp. The strain is separated from an oil reservoir source, can reduce interfacial tension between crude oil and water, plays a role in emulsifying the crude oil, is good in oil reservoir adaptability, and can convert the crude oil into methane in an extreme environment of the oil reservoir and in the presence of CO2. The invention provides a new high-quality strain resource for microbial oil recovery, and has great significance for improving the oil recovery rate, reducing carbon emission by CCUS microbial carbon conversion and treating and repairing petroleum pollution.

The application discloses a benzo[a]pyrene-degrading fungus Arthrinium acutiapicum DL-5 in petroleum contaminated soil, a microbial inoculum of the Arthrinium acutiapicum DL-5 and application of the Arthrinium acutiapicum DL-5. ‑1 The benzo[a]pyrene-degrading rate of the pure Arthrinium acutiapicum DL-5 to benzo[a]pyrene can reach more than 75% after the Arthrinium acutiapicum DL-5 is cultured in an inorganic salt culture solution with an initial concentration of 25 mg / L of benzo[a]pyrene for 7 days. After the Arthrinium acutiapicum DL-5 is made into a microbial inoculum, the benzo[a]pyrene-degrading rate of the Arthrinium acutiapicum DL-5 to benzo[a]pyrene can be promoted to more than 80%, and therefore, the Arthrinium acutiapicum DL-5 and the microbial inoculum have good application potential in the bioremediation of polycyclic aromatic hydrocarbons.

The present invention provides a polar low-temperature surfactant-producing bacterium Pedobacter cryoconitis NJ‑S‑72 and its application, which belongs to the field of bioremediation technology for petroleum and petroleum product contaminated environments. The strain was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on October 31, 2024, with a deposit number of CGMCC NO: 32458. The strain is derived from the soil of the Fildes Peninsula in Antarctica, and its fermentation liquid can be directly applied to the ecological restoration of oil spills in polar or low-temperature environments. Compared with chemical surfactants, it can reduce secondary damage to the environment, and the biosurfactant produced by the strain can accelerate the emulsification of oil and accelerate the biodegradation of oil pollution.

The invention discloses an industrial application device and method for microbe enhanced ex-situ remediation of petroleum-contaminated soil by applying ozone sequencing batch driving. According to the method, ozone and air with a certain concentration are intermittently and circularly injected into the soil containing the petroleum pollutants in an ectopic mode for a specific time period, and the contaminated soil is remedied through ozone oxidation coupled with microorganisms. According to the invention, ozone is injected to decompose organic pollutants which are difficult to degrade in petroleum into short-chain organic matters with good biodegradability by utilizing relatively strong oxidability and penetrability of ozone, so that the bioavailability of the pollutants is improved; air injection promotes microorganisms to decompose pollutants difficult to degrade in the soil, and alternate synergy of chemical oxidation and bioremediation is achieved through a sequencing batch type gas circulation injection mode, so that the overall remediation efficiency is improved, and then ectopic harmless treatment of the contaminated soil is achieved.

The invention discloses a petroleum-contaminated soil remediation agent based on synergism of a fixed microbial inoculum and free bacteria and application of the petroleum-contaminated soil remediation agent, and belongs to the technical field of soil pollution control and treatment. The petroleum-contaminated soil remediation agent is prepared from an immobilized microbial agent prepared by immobilizing stenotrophomonas by adopting rape straw biochar and free pseudomonas, wherein the immobilized microbial agent is prepared by immobilizing stenotrophomonas by adopting rape straw biochar; wherein both the stenotrophomonas and the pseudomonas are preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the stenotrophomonas and the pseudomonas are respectively CGMCC No.34532 and CGMCC No.34533. According to the method, the petroleum-contaminated soil is repaired by adopting a technology of combining the immobilized microbial inoculum and the free degrading bacteria for the first time, so that the advantages of the immobilized microbial inoculum are reserved, and the degrading effect is improved through the synergistic effect of the immobilized microbial inoculum and the free bacteria. In addition, an adsorption method is used for immobilization, operation is simple, chemical reagents such as an embedding agent and a cross-linking agent are not added, and secondary pollution is avoided.

The application discloses a strain of Acinetobacter, the strain name is C16ZT1, and simultaneously proposes a bacterial agent containing the different bacillus, and further uses the bacterial agent for treating petroleum pollution wastewater. The Acinetobacter can efficiently degrade the petroleum production wastewater containing chain alkanes, can still realize the degradation effect quickly and stably under the conditions of high concentration, high salt and extreme acid and alkali, and can degrade more than 95% of the petroleum production wastewater within 144 hours. In the application in sewage treatment, the strain has strong tolerance, can tolerate high-concentration alkanes, and has a wide adaptation range to salt concentration, pH and the like, and has a good application prospect.

The present application relates to the technical field of marine oil pollution degradation, in particular to a microbial remediation agent for marine oil pollution degradation and a preparation method thereof, which is used to solve the problem that the existing biological remediation method is difficult to play an effective degradation role in the complex marine environment; the microbial remediation agent takes hydrophobic microspheres coated carbon fixed bacteria agent and modified composite particles as main raw materials, combines the dual degradation mechanisms of microbial degradation and photocatalytic degradation, significantly improves the degradation effect and efficiency of oil pollutants, has good stability, prolongs the service life, and the selected materials have excellent environmental protection performance and will not cause secondary pollution to the marine environment, so it has a broad application prospect.

The invention discloses a Pandoraea sp. YDY-3 strain and an application of the Pandoraea sp. YDY-3 strain in degradation of n-hexadecane. The Pandoraea pandurata YDY-3 is named as Pandoraea pandurata YDY-3, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 20251289. The Pandoraea pandurata YDY-3 is named as Pandoraea pandurata YDY-3. The Pandoraea sp. YDY-3 has the capability of degrading n-hexadecane under the condition that the pH value is 7.0-10.0, and can grow at 28-32 DEG C. The Pandoraea sp. YDY-3 has the advantages that the Pandoraea sp. The Pandoraea sp. YDY-3 provided by the invention has relatively strong environmental adaptability and n-hexadecane degradation capability, and provides a foundation for bioremediation of petroleum polluted environment and application of a microbial enhanced treatment technology.

The invention discloses bacillus velezensis BZ capable of degrading petroleum hydrocarbon, the name of the strain is BZ, the classification name is bacillus velezensis, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31766. The invention further discloses a preparation method of the bacillus velezensis BZ capable of degrading petroleum hydrocarbon. The invention discloses an application of bacillus velezensis capable of degrading petroleum hydrocarbon in degradation of petroleum hydrocarbon. The BZ strain provided by the invention can grow by taking petroleum hydrocarbon as a sole carbon source, can tolerate the low temperature of 5 DEG C and salinity with the concentration of 10%, and can quickly degrade the petroleum hydrocarbon, so that the BZ strain can be widely applied to petroleum degradation. In addition, the strain is simple in culture method and high in tolerant petroleum hydrocarbon concentration, and can be directly used for treating petroleum-polluted water and soil.

The invention discloses a preparation method of attapulgite modified biochar and application of the attapulgite modified biochar in petroleum pollution remediation, and relates to the technical field of environmental geochemistry. The attapulgite modified biochar provided by the invention can keep good adsorption performance on petroleum hydrocarbon in a saline-alkali environment, can be successfully applied to removal of hydrocarbon such as petroleum hydrocarbon, and can gradually break n-alkanes with relatively large carbon atoms to form n-alkanes with relatively small carbon atoms; aromatic hydrocarbon can be subjected to oxidation and ring-opening reaction to generate hydrocarbon with fewer carbon atoms, a petroleum-polluted sample is treated by the attapulgite modified biochar, the adsorption rate of petroleum hydrocarbon in the sample can be greatly improved, and the adsorption rates of the modified biochar BA400 and BA700 on petroleum hydrocarbon pollution in a saline-alkali environment are respectively improved by 17.66% and 26.29% compared with those of BC400 and BC700. Therefore, the attapulgite modified biochar provides a new method for treatment of saline alkali soil polluted by petroleum hydrocarbon.

The invention discloses an efficient carbon sequestration bacillus cereus from an oil-containing solid waste residue source and application of the efficient carbon sequestration bacillus cereus. The bacillus cereus is any one of bacillus cereus S13 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 2024515 and bacillus cereus S29 with the preservation number of CCT CC NO: M 2024516. The bacillus cereus strain is obtained by taking bacillus cereus S10 separated from oil-containing solid waste residues of an oil field as an original strain through ARTP mutagenesis and screening, has excellent characteristics of reducing pollution and sequestering carbon, is relatively strong in stress resistance, can be used for deeply recycling the oil-containing solid waste residues, can be used for improving the carbon sequestration capacity of petroleum-polluted soil and can be used for preparing the oil-containing solid waste residues. The method has a wide application prospect in the fields of petroleum-polluted soil remediation and the like, and has great significance in pollution reduction and convergence increase in petroleum and petrochemical industries under the background of supporting double carbon.

The invention discloses separation and identification of a yeast Rhodotorula mucilaginosa DY-1 for degrading phenanthrene and benzopyrene in petroleum contaminated soil, and an application of the yeast Rhodotorula mucilaginosa DY-1 for degrading phenanthrene and benzopyrene in petroleum contaminated soil. The strain Rhodotorula mucilaginosa DY-1 is domesticated, separated and identified from petroleum-polluted soil in Ningbo City in Zhejiang Province, phenanthrene and benzopyrene can be used as carbon sources, and after the strain is cultured for 7 days in an inorganic salt culture solution with the initial concentration of phenanthrene and benzopyrene being 50 mg.L <-1 > and 25 mg.L <-1 > respectively, the degradation rate of phenanthrene and benzopyrene can reach 70% or above. Therefore, the strain has good application potential in the aspect of bioremediation of polycyclic aromatic hydrocarbon.

The invention discloses a method for promoting aqueous-phase hydroxyl free radicals to migrate to soil to directly oxidize petroleum hydrocarbons, which comprises the following steps: mixing a chitin mixture, a citric acid-ferrous sulfate solution and H2O2 water to react to prepare a Fe-SOM material; mixing the Fe-SOM material with petroleum-contaminated soil, performing rotary oscillation, regulating and controlling the relative polarity of the soil, and guiding hydroxyl radicals to directionally migrate from a water phase to a solid phase to obtain a Fe-SOM regulated and controlled soil material; h2O2 is added into the Fe-SOM regulated soil material for continuous oscillation, and the process of directly oxidizing the petroleum hydrocarbon in the soil is completed. Introduction of organic functional groups is changed by regulating materials, the organic matter content of soil is increased, and therefore the relative polarity of the soil is improved; by improving the relative polarity of the soil, the transfer ratio of hydroxyl radicals is increased, the relative polarity eta value of the soil and the direct oxidation amount of TPH are increased, petroleum is directly oxidized in a solid phase, and therefore the effect of Fenton petroleum oxidation is improved.

The invention belongs to the field of comprehensive treatment of oily sludge, and relates to a high-temperature-resistant petroleum degrading bacterium, application and an oily sludge degrading method. The high-temperature-resistant petroleum degrading bacterium is geobacillus stearothermophilus, and the preservation number of the high-temperature-resistant petroleum degrading bacterium is CGMCC (China General Microbiological Culture Collection Center) No. 29411. According to the method, high-temperature-resistant microorganisms are screened, enriched and amplified by utilizing oil sludge or oil-polluted soil. The flora screened by the method is more targeted when being used for treating the oil sludge, the rapid and efficient degradation of the oil-containing sludge can be realized, and a better oil sludge treatment effect is achieved.

This invention relates to a petroleum hydrocarbon-degrading bacterium and its application, belonging to the field of microbial technology. To address the technical problems in existing petroleum-contaminated soil bioremediation, such as the scarcity of highly efficient degrading strains, low degradation efficiency, and insufficient capacity to handle complex hydrocarbons, this invention provides a petroleum hydrocarbon-degrading bacterium, *Acinetobacter pylori* AFRC34, with accession number CCTCC No:2026007. This strain can use petroleum hydrocarbon organic matter in diesel fuel as the sole carbon source and grows on inorganic salt culture media. It maintains a high petroleum hydrocarbon degradation rate even at a low temperature of 15℃. In an inorganic salt culture medium containing 10,000 mg / L high-concentration diesel fuel at 37℃, the degradation rate reaches 92.46%. The strain and inoculant provided by this invention can efficiently degrade petroleum hydrocarbon pollutants, and are particularly suitable for in-situ bioremediation in cold regions and heavily contaminated sites.

This invention provides a strain of Bacillus thuringiensis NH26 and its applications. This strain was deposited on July 21, 2022, at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC NO: M20221149, located at Wuhan University, Wuhan, China. The Bacillus thuringiensis NH26 strain provided by this invention exhibits excellent degradation capabilities for various petroleum hydrocarbons, including alkanes and polycyclic aromatic hydrocarbons, under high salinity conditions such as seawater and other high-salinity bodies. Even under high concentrations of petroleum / petroleum hydrocarbons, it still demonstrates excellent degradation performance, making it of significant value in the remediation of petroleum pollution in high-salinity bodies such as marine environments.

The invention discloses a parsley fungus YDY-1 strain and an application of the parsley fungus YDY-1 strain in degradation of n-hexadecane. According to the present invention, the apiotrichum cotoxinovorans YDY-1 is named as the apiotrichum cotoxinovorans YDY-1, and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC M 20251287, and the preservation number of the apiotrichum cotoxinovorans YDY-1 is CCTCC M 20251287; the parvibrio molitor YDY-1 provided by the invention has the capability of degrading n-hexadecane under the condition that the pH (Potential of Hydrogen) is 7.0 to 9.0, and can grow at 28 to 32 DEG C. The parvibrio molitor YDY-1 provided by the invention has relatively strong environmental adaptability and n-hexadecane degradation capability, and provides a basis for bioremediation of petroleum polluted environment and application of a microbial enhanced treatment technology.

The application discloses a kind of petroleum pollution site typical organic pollution tracing analysis system and method, contain a kind of NMFCMB composite receptor system.The system includes NMF module, coupling module and CMB module.The method includes the following steps: S1, data preprocessing is carried out using NMF module;S2, main component factor is extracted using NMF module;S3, non-negative constraint factor decomposition is carried out using NMF module;S4, main pollution source is analyzed using coupling module;S5, pollution source contribution rate is calculated and uncertainty analysis is carried out using CMB module;S6, iteration calculation is carried out repeatedly to step S1 to S5, until the convergence condition is satisfied.The application solves the problem of poor accuracy of source analysis results and poor stability of the number of pollution sources in the prior art.

The invention discloses a composite biological stimulation agent for stimulating activity of a petroleum hydrocarbon degrading enzyme, which comprises the following components in parts by weight: 5 to 8 parts of KNO3, 5 to 10 parts of FeSO4, 1 to 8 parts of MnSO4, 1000 parts of rhamnolipid and 20 parts of succinic acid. The method has the beneficial effects that the spanning of an in-situ bioremediation technology of the petroleum-polluted underground water from experience to quantification and mechanization is promoted, and the method has important theoretical value and engineering application prospect. The restoration efficiency is improved in magnitude, and the petroleum hydrocarbon degradation rate within 7 days is improved to 75% from 30% under the natural attenuation condition.

This invention discloses a microbial composting treatment device for petroleum-contaminated soil, comprising: a composting tank and a stirring and oxygenation device. The composting tank has a receiving cavity, an inlet, and an outlet. The stirring and oxygenation device includes a spiral auger and an air guide pipe. A driven gear is located at the bottom of the spiral auger. The first cavity of the air guide pipe is connected to the air inlet of the air guide pipe. The air guide pipe has multiple first through holes communicating with the receiving cavity, with both ends of the first through holes connecting the receiving cavity and the first cavity of the air guide pipe, respectively. During the tumbling process, the spiral auger promotes the homogenization of pollutant concentration throughout the compost pile, thereby improving the microbial degradation efficiency. The air guide pipe extends along the axis of the spiral auger and rotates synchronously with it. It is connected to an external fan via a rotary joint, allowing air to pass through the first cavity of the air guide pipe and then be evenly dispersed into the soil compost pile through the multiple first through holes. This achieves synergistic operation of tumbling and oxygenation, enabling oxygen to reach deep areas of the compost pile.