36 results about "Petroleum Pollution" patented technology

The invention discloses a flora prediction and petroleum pollution remediation method based on machine learning. The method comprises the following steps: collecting multiple groups of experimental data of a diesel oil pollution sample treated by a microbial agent, extracting environmental factors, microbial community characteristics and target response variables to construct a training data set after missing value processing, abnormal value detection and standardized pretreatment, and importing the data set into a preset machine learning model to obtain a training result; carrying out feature learning, classification training and hyper-parameter optimization by adopting a GridSearchCV method in combination with 10-fold cross validation; evaluating the correlation between a target response variable classification result and the features through a multivariable Pearson's correlation matrix, and constructing an optimal test set; and finally, selecting an optimal prediction model according to a preset index. According to the method, the model training quality is improved through data preprocessing and correlation analysis, efficient flora prediction and algorithm application evaluation are achieved by means of multiple machine learning algorithms, scientific support is provided for petroleum pollution remediation, and remediation accuracy and efficiency are improved.

The invention is applicable to the technical field of industrial wastewater treatment, and provides a construction method of a microalgae-in-situ bacteria strengthening system for degrading naphthenic acid, which comprises the following steps: separating and identifying two strains of in-situ bacteria from a discharge port of an oil-producing wastewater treatment plant, and constructing a three-strain typical stain-resistant green alga-in-situ bacteria co-culture system. The strengthening system can highly tolerate and efficiently degrade naphthenic acid, can effectively remove nitrogen and phosphorus, and has higher biomass and high-value compound yield. The microalgae-in-situ bacterium strengthening system can reduce pollutant discharge and improve the microalgae resource utilization rate, and the microalgae-in-situ bacterium strengthening system can be used as a biological strengthening degradation technology of naphthenic acid and can be suitable for purification treatment of oil production wastewater and petroleum polluted water areas.

The invention belongs to the technical field of solid waste recycling, and discloses efficient carbon sequestration pseudomonas stutzeri from an oil-containing solid waste residue source and application of the efficient carbon sequestration pseudomonas stutzeri, and the efficient carbon sequestration pseudomonas stutzeri is pseudomonas stutzeri A58-5 with the preservation number of CCTCC NO: M 20241582. The Pseudomonas stutzeri is obtained by taking Pseudomonas stutzeri A58 separated from oil-containing solid waste residues of an oil field as an original strain and carrying out ARTP mutagenesis and screening, has the excellent characteristics of reducing pollution and sequestering carbon and is relatively strong in stress resistance; the method has wide application prospects in the fields of deep recycling (soil conversion) of oil-containing solid waste residues, improvement of the carbon sequestration capacity of the petroleum-contaminated soil, remediation of the petroleum-contaminated soil and the like, and is of great significance in pollution reduction and sequestration increase in the petroleum and petrochemical industry under the background of supporting double carbon.

The invention discloses trichoderma longibrachiatum for degrading petroleum hydrocarbon in a saline-alkali environment, a culture and application of the trichoderma longibrachiatum, and relates to the technical field of microorganisms. The preservation number of the trichoderma longibrachiatum is CGMCC (China General Microbiological Culture Collection Center) No. The trichoderma longibrachiatum provided by the invention has an efficient petroleum degradation characteristic, provides a practical and effective solution for the petroleum pollution problem of the saline-alkali soil, can efficiently degrade petroleum hydrocarbon in the saline-alkali soil, and has a good application prospect.

This invention discloses a benzo[a]pyrene-degrading fungus, *Nigrospora osmanthi* DL-7, for use in petroleum-contaminated soil, along with its inoculum and applications. This invention domesticated, isolated, and identified a benzo[a]pyrene-degrading strain, *Nigrospora osmanthi* DL-7, from petroleum-contaminated soil in Ningbo. DL-7 can utilize benzo[a]pyrene as a carbon source, even at an initial benzo[a]pyrene concentration of 25 mg·L⁻¹. ‑1 After culturing in an inorganic salt broth for 7 days, the pure DL-7 strain achieved a degradation rate of over 85% for benzo[a]pyrene. When prepared as a bacterial agent, the degradation rate of benzo[a]pyrene could be increased to over 90%. Therefore, this strain and its bacterial agent have good application potential in the bioremediation of polycyclic aromatic hydrocarbons.

The invention belongs to the technical field of oil reservoir microorganisms, and particularly relates to a zoococcus strain and application thereof. The bacterial strain is a Planococcus sp. Bacterial strain WL-9, and is a new strain of the Planococcus sp. The strain is separated from an oil reservoir source, can reduce interfacial tension between crude oil and water, plays a role in emulsifying the crude oil, is good in oil reservoir adaptability, and can convert the crude oil into methane in an extreme environment of the oil reservoir and in the presence of CO2. The invention provides a new high-quality strain resource for microbial oil recovery, and has great significance for improving the oil recovery rate, reducing carbon emission by CCUS microbial carbon conversion and treating and repairing petroleum pollution.

The invention discloses an industrial application device and method for microbe enhanced ex-situ remediation of petroleum-contaminated soil by applying ozone sequencing batch driving. According to the method, ozone and air with a certain concentration are intermittently and circularly injected into the soil containing the petroleum pollutants in an ectopic mode for a specific time period, and the contaminated soil is remedied through ozone oxidation coupled with microorganisms. According to the invention, ozone is injected to decompose organic pollutants which are difficult to degrade in petroleum into short-chain organic matters with good biodegradability by utilizing relatively strong oxidability and penetrability of ozone, so that the bioavailability of the pollutants is improved; air injection promotes microorganisms to decompose pollutants difficult to degrade in the soil, and alternate synergy of chemical oxidation and bioremediation is achieved through a sequencing batch type gas circulation injection mode, so that the overall remediation efficiency is improved, and then ectopic harmless treatment of the contaminated soil is achieved.

The invention discloses a petroleum-contaminated soil remediation agent based on synergism of a fixed microbial inoculum and free bacteria and application of the petroleum-contaminated soil remediation agent, and belongs to the technical field of soil pollution control and treatment. The petroleum-contaminated soil remediation agent is prepared from an immobilized microbial agent prepared by immobilizing stenotrophomonas by adopting rape straw biochar and free pseudomonas, wherein the immobilized microbial agent is prepared by immobilizing stenotrophomonas by adopting rape straw biochar; wherein both the stenotrophomonas and the pseudomonas are preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the stenotrophomonas and the pseudomonas are respectively CGMCC No.34532 and CGMCC No.34533. According to the method, the petroleum-contaminated soil is repaired by adopting a technology of combining the immobilized microbial inoculum and the free degrading bacteria for the first time, so that the advantages of the immobilized microbial inoculum are reserved, and the degrading effect is improved through the synergistic effect of the immobilized microbial inoculum and the free bacteria. In addition, an adsorption method is used for immobilization, operation is simple, chemical reagents such as an embedding agent and a cross-linking agent are not added, and secondary pollution is avoided.

The application discloses a strain of Acinetobacter, the strain name is C16ZT1, and simultaneously proposes a bacterial agent containing the different bacillus, and further uses the bacterial agent for treating petroleum pollution wastewater. The Acinetobacter can efficiently degrade the petroleum production wastewater containing chain alkanes, can still realize the degradation effect quickly and stably under the conditions of high concentration, high salt and extreme acid and alkali, and can degrade more than 95% of the petroleum production wastewater within 144 hours. In the application in sewage treatment, the strain has strong tolerance, can tolerate high-concentration alkanes, and has a wide adaptation range to salt concentration, pH and the like, and has a good application prospect.

The present application relates to the technical field of marine oil pollution degradation, in particular to a microbial remediation agent for marine oil pollution degradation and a preparation method thereof, which is used to solve the problem that the existing biological remediation method is difficult to play an effective degradation role in the complex marine environment; the microbial remediation agent takes hydrophobic microspheres coated carbon fixed bacteria agent and modified composite particles as main raw materials, combines the dual degradation mechanisms of microbial degradation and photocatalytic degradation, significantly improves the degradation effect and efficiency of oil pollutants, has good stability, prolongs the service life, and the selected materials have excellent environmental protection performance and will not cause secondary pollution to the marine environment, so it has a broad application prospect.

The invention discloses bacillus velezensis BZ capable of degrading petroleum hydrocarbon, the name of the strain is BZ, the classification name is bacillus velezensis, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31766. The invention further discloses a preparation method of the bacillus velezensis BZ capable of degrading petroleum hydrocarbon. The invention discloses an application of bacillus velezensis capable of degrading petroleum hydrocarbon in degradation of petroleum hydrocarbon. The BZ strain provided by the invention can grow by taking petroleum hydrocarbon as a sole carbon source, can tolerate the low temperature of 5 DEG C and salinity with the concentration of 10%, and can quickly degrade the petroleum hydrocarbon, so that the BZ strain can be widely applied to petroleum degradation. In addition, the strain is simple in culture method and high in tolerant petroleum hydrocarbon concentration, and can be directly used for treating petroleum-polluted water and soil.

The invention discloses a preparation method of attapulgite modified biochar and application of the attapulgite modified biochar in petroleum pollution remediation, and relates to the technical field of environmental geochemistry. The attapulgite modified biochar provided by the invention can keep good adsorption performance on petroleum hydrocarbon in a saline-alkali environment, can be successfully applied to removal of hydrocarbon such as petroleum hydrocarbon, and can gradually break n-alkanes with relatively large carbon atoms to form n-alkanes with relatively small carbon atoms; aromatic hydrocarbon can be subjected to oxidation and ring-opening reaction to generate hydrocarbon with fewer carbon atoms, a petroleum-polluted sample is treated by the attapulgite modified biochar, the adsorption rate of petroleum hydrocarbon in the sample can be greatly improved, and the adsorption rates of the modified biochar BA400 and BA700 on petroleum hydrocarbon pollution in a saline-alkali environment are respectively improved by 17.66% and 26.29% compared with those of BC400 and BC700. Therefore, the attapulgite modified biochar provides a new method for treatment of saline alkali soil polluted by petroleum hydrocarbon.

The invention discloses a method for promoting aqueous-phase hydroxyl free radicals to migrate to soil to directly oxidize petroleum hydrocarbons, which comprises the following steps: mixing a chitin mixture, a citric acid-ferrous sulfate solution and H2O2 water to react to prepare a Fe-SOM material; mixing the Fe-SOM material with petroleum-contaminated soil, performing rotary oscillation, regulating and controlling the relative polarity of the soil, and guiding hydroxyl radicals to directionally migrate from a water phase to a solid phase to obtain a Fe-SOM regulated and controlled soil material; h2O2 is added into the Fe-SOM regulated soil material for continuous oscillation, and the process of directly oxidizing the petroleum hydrocarbon in the soil is completed. Introduction of organic functional groups is changed by regulating materials, the organic matter content of soil is increased, and therefore the relative polarity of the soil is improved; by improving the relative polarity of the soil, the transfer ratio of hydroxyl radicals is increased, the relative polarity eta value of the soil and the direct oxidation amount of TPH are increased, petroleum is directly oxidized in a solid phase, and therefore the effect of Fenton petroleum oxidation is improved.

The invention belongs to the field of comprehensive treatment of oily sludge, and relates to a high-temperature-resistant petroleum degrading bacterium, application and an oily sludge degrading method. The high-temperature-resistant petroleum degrading bacterium is geobacillus stearothermophilus, and the preservation number of the high-temperature-resistant petroleum degrading bacterium is CGMCC (China General Microbiological Culture Collection Center) No. 29411. According to the method, high-temperature-resistant microorganisms are screened, enriched and amplified by utilizing oil sludge or oil-polluted soil. The flora screened by the method is more targeted when being used for treating the oil sludge, the rapid and efficient degradation of the oil-containing sludge can be realized, and a better oil sludge treatment effect is achieved.

This invention relates to a petroleum hydrocarbon-degrading bacterium and its application, belonging to the field of microbial technology. To address the technical problems in existing petroleum-contaminated soil bioremediation, such as the scarcity of highly efficient degrading strains, low degradation efficiency, and insufficient capacity to handle complex hydrocarbons, this invention provides a petroleum hydrocarbon-degrading bacterium, *Acinetobacter pylori* AFRC34, with accession number CCTCC No:2026007. This strain can use petroleum hydrocarbon organic matter in diesel fuel as the sole carbon source and grows on inorganic salt culture media. It maintains a high petroleum hydrocarbon degradation rate even at a low temperature of 15℃. In an inorganic salt culture medium containing 10,000 mg / L high-concentration diesel fuel at 37℃, the degradation rate reaches 92.46%. The strain and inoculant provided by this invention can efficiently degrade petroleum hydrocarbon pollutants, and are particularly suitable for in-situ bioremediation in cold regions and heavily contaminated sites.

The invention discloses a composite biological stimulation agent for stimulating activity of a petroleum hydrocarbon degrading enzyme, which comprises the following components in parts by weight: 5 to 8 parts of KNO3, 5 to 10 parts of FeSO4, 1 to 8 parts of MnSO4, 1000 parts of rhamnolipid and 20 parts of succinic acid. The method has the beneficial effects that the spanning of an in-situ bioremediation technology of the petroleum-polluted underground water from experience to quantification and mechanization is promoted, and the method has important theoretical value and engineering application prospect. The restoration efficiency is improved in magnitude, and the petroleum hydrocarbon degradation rate within 7 days is improved to 75% from 30% under the natural attenuation condition.

This invention discloses a microbial composting treatment device for petroleum-contaminated soil, comprising: a composting tank and a stirring and oxygenation device. The composting tank has a receiving cavity, an inlet, and an outlet. The stirring and oxygenation device includes a spiral auger and an air guide pipe. A driven gear is located at the bottom of the spiral auger. The first cavity of the air guide pipe is connected to the air inlet of the air guide pipe. The air guide pipe has multiple first through holes communicating with the receiving cavity, with both ends of the first through holes connecting the receiving cavity and the first cavity of the air guide pipe, respectively. During the tumbling process, the spiral auger promotes the homogenization of pollutant concentration throughout the compost pile, thereby improving the microbial degradation efficiency. The air guide pipe extends along the axis of the spiral auger and rotates synchronously with it. It is connected to an external fan via a rotary joint, allowing air to pass through the first cavity of the air guide pipe and then be evenly dispersed into the soil compost pile through the multiple first through holes. This achieves synergistic operation of tumbling and oxygenation, enabling oxygen to reach deep areas of the compost pile.

The present application relates to a kind of biological eluent composition applied to severe oil-contaminated soil remediation and oil-containing sludge treatment, wherein, based on the total mass of the composition, it contains: (1) biological surfactant, content is 0.05-5.0wt%; (2) synergist, content is 0.02-5.0wt%; (3) ion regulator, content is 0.02-3.0wt%; (4) the rest is mainly water; the biological surfactant is selected from one or more of rhamnolipid, esterified modified sophorose ester and sulfonated modified sophorolipid, the synergist is peroxide, and the ion regulator is selected from nitrogen-phosphorus type inorganic salt. The eluent can effectively separate the oil phase in the severe oil-contaminated soil and the high oil-containing sludge, reduce the adhesion of the oil phase on the surface of soil particles, enhance the solubility of the oil phase in the solution, and has the advantages of high efficiency, short processing cycle and easy biodegradation.

The invention discloses a method for repairing petroleum-contaminated soil through a biochar reinforced alternating current-microorganism technology, and belongs to the technical field of soil repairing. The method comprises the following steps: preparing sludge-based biochar or modified sludge-based biochar; preparing a compound microbial agent; the method comprises the following steps: adding polluted soil into a reactor, adjusting the water content, adding sludge-based biochar or modified biochar, adding a compound microbial agent, inserting an electrode, applying an electric field, and repairing the petroleum-polluted soil. According to the method, the removal rate of petroleum pollutants is remarkably increased, the remediation period is shortened, and a feasible method is provided for green and efficient remediation of the petroleum-contaminated soil.

The invention belongs to the technical field of microbial degradation of petroleum pollution, and particularly discloses bacillus velezensis and application thereof in remediation of a petroleum hydrocarbon polluted environment. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.29578. The strain has the characteristics of using petroleum hydrocarbon as a substrate and metabolizing a biological surfactant, and has relatively strong degradation performance on petroleum hydrocarbon, especially heavy hydrocarbon. Experimental results show that the strain can reduce the surface tension of crude oil from 72.326 mN / m to 27.392 mN / m within 7 days, the 7-day degradation rate of petroleum hydrocarbon in oil-containing sewage is 37.11%, and the 7-day degradation rate of heavy long chain hydrocarbon in the oil-containing sewage reaches 96.41%.

The invention belongs to the field of oil and gas field environmental protection equipment, and relates to a petroleum-contaminated soil microbiological treatment self-ploughing system and method, which are suitable for performing petroleum-contaminated soil microbiological treatment by adopting a composting process. The soil above is pushed to displace through gradual expansion of the ploughing rubber air bag, automatic ploughing in the treatment process is achieved, the oxygen content in the soil is increased, the growth and reproduction environment of microorganisms is improved, and therefore the biodegradation process of petroleum hydrocarbon is accelerated.

This invention discloses a method for preparing a dual-emulsion sustained-release agent for remediating petroleum-contaminated groundwater, belonging to the field of petroleum pollution control. The method includes preparing a nutrient matrix, preparing a dual-emulsion nutrient sustained-release agent, and preparing an outer shell structure. The specific steps for preparing the dual-emulsion nutrient sustained-release agent are as follows: Step 1, obtaining an inner aqueous phase W1; Step 2, obtaining an experimental oil phase O; Step 3, stirring to form a W1 / O type emulsion; Step 4, obtaining an outer aqueous phase W2; Step 5, obtaining the dual-emulsion nutrient sustained-release agent. The specific steps for preparing the outer shell structure are as follows: Step 1, heating to form a gelling agent; Step 2, the ratio of the gelling agent to W1 / O / W2 is 5:1; Step 3, water bath heating; Step 4, solution separation; Step 5, obtaining a boric acid solution; Step 6, forming spherical structures in the solution; Step 7, obtaining the dual-emulsion sustained-release agent. Beneficial effects: It can efficiently degrade petroleum hydrocarbons in groundwater, improving the petroleum hydrocarbon degradation effect by more than 40%.

The invention relates to a degradation catalyst as well as a preparation method and application thereof, and relates to the technical field of pollution remediation. The degradation catalyst comprises a zero-valent iron-porous material compound and petroleum degrading bacteria, the petroleum degrading bacteria are immobilized on the zero-valent iron-porous material compound. According to the method, a solid-phase Fenton oxidation method and an immobilized microbiological method are coupled and have a synergistic effect, efficient and rapid remediation of the petroleum-contaminated soil is achieved, the process is simple, and the method has an excellent application prospect in in-situ remediation of the petroleum-contaminated soil.

The invention belongs to the field of comprehensive treatment of oily sludge, and relates to a medium-high temperature petroleum degrading bacterium, application and an oily sludge degrading method. The medium and high temperature petroleum degrading bacterium is brevibacillus borstelensis, and the preservation number of the medium and high temperature petroleum degrading bacterium is CGMCC (China General Microbiological Culture Collection Center) No. 29416. According to the invention, petrophilic bacteria existing in oil sludge or soil polluted by petroleum are utilized to screen, enrich and enlarge high-temperature microbial bacteria in culture for degrading oily sludge. The strain screened by the method is more targeted when being used for treating the oil sludge, rapid and efficient degradation of the oil-containing sludge can be realized, and a better oil sludge treatment effect is achieved.

This application relates to the field of analytical chemistry technology and provides a method for detecting perfluorinated and polyfluoroalkane (PFAS) compounds in petroleum-contaminated soil. The method includes: freeze-drying, pulverizing, and sieving the petroleum-contaminated soil; mixing the sieved soil with diatomaceous earth, placing it in an extraction cell for extraction, and collecting the extract; drying the extract, redissolving it with methanol, filtering, and diluting to obtain the sample to be tested; detecting the sample using high-performance liquid chromatography-high-resolution mass spectrometry (HPLC-MS / MS) in negative ion electrospray ionization mode; and identifying compounds based on molecular ion mass, retention time, and peak shape. This application provides a standardized detection method adapted to petroleum-based soil matrices, integrating targeted precise qualitative analysis and non-targeted broad-spectrum screening of PFAS based on rapid solvent extraction and HPLC-MS / MS, solving the problems of low extraction efficiency, poor purification effect, and inaccurate qualitative analysis of PFAS trace detection under strong interference from petroleum hydrocarbons.

The invention relates to the technical field of heat soil remediation, and discloses a treatment device and method for soil organic pollution remediation, the treatment device for soil organic pollution remediation comprises a soil heat treatment box, and a partition treatment mechanism is arranged in the soil heat treatment box; the partition treatment mechanism comprises a gas lifting heat treatment box, a microwave treatment box and a heat energy supply assembly, the gas lifting heat treatment box and the microwave treatment box are sequentially installed in the advancing direction of the soil in the soil heat treatment box, and the soil polluted by petroleum sequentially passes through the gas lifting heat treatment box and then passes through the microwave treatment box; soil shoveling plates are arranged in the air lifting heat treatment box and the microwave treatment box in an array mode and used for shoveling moving soil, and a plurality of air spraying heads are arranged below the soil shoveling plates in the air lifting heat treatment box in an array mode and connected with a heat energy supply assembly; according to the invention, the soil treatment process is divided into two stages of gas-lifting heat treatment and microwave gas explosion treatment, so that the step-by-step deep removal of pollutants is realized.

The invention belongs to the technical field of oily sludge bioremediation, and particularly relates to a petroleum hydrocarbon degradation composite flora and application thereof, and the composite flora comprises P.megatherium OS-A1 and Bacillus tropicalis OS-D5; the P.megatherium OS-A1 is preserved in the China Center for Type Culture Collection on June 17, 2025, and the biological preservation number of the P.megatherium OS-A1 is CCTCC NO: M 20251408; the bacillus tropicalis OS-D5 is preserved in the China Center for Type Culture Collection on June 17, 2025, and the biological preservation number of the bacillus tropicalis OS-D5 is CCTCC NO: M 20251409. According to the invention, the technical problems of poor flora stability and incomplete metabolic substrate coverage in the prior art are solved, the coverage of full carbon chain degradation is realized, the limitation of a single strain on specific hydrocarbon degradation is avoided, and a breakthrough solution is provided for complex petroleum pollution remediation.

The invention discloses trehalose lipid modified biochar and a preparation method and application thereof.The preparation method of the trehalose lipid modified biochar comprises the steps that trehalose lipid and water are mixed to be uniform, and a trehalose lipid solution is obtained; the preparation method comprises the following steps: uniformly mixing a trehalose lipid solution and biochar, standing, drying, cooling to room temperature to obtain a black solid, cleaning the black solid to be neutral, drying, and sieving for the first time to obtain the trehalose lipid modified biochar. The trehalose lipid modified biochar is added into the petroleum-contaminated soil, and the petroleum-contaminated soil is repaired by strengthening microorganisms. The addition of the trehalose lipid modified biochar can obviously improve the physicochemical properties of the petroleum-contaminated soil, provide a good growth environment for microorganisms, increase the bioavailability of petroleum hydrocarbon in the soil, reduce the stress effect of petroleum pollutants on the microorganisms, and significantly improve the biodegradation rate of petroleum hydrocarbon in the petroleum-contaminated soil.

The invention relates to the technical field of environmental engineering, and discloses a soil remediation simulation method and system based on Aspen Plus. Petroleum-contaminated soil is added into a tubular furnace, thermal desorption remediation and resource recovery experiments are conducted in the nitrogen atmosphere, and gas-liquid-solid products are collected after reaction; the method comprises the following steps: establishing a simulation model for a PCS thermal desorption repair and resource recovery process by adopting Aspen Plus, inputting experimental parameters into the simulation model for simulation to obtain a group of simulation results, comparing the obtained simulation results with an experiment, and verifying the feasibility of the simulation model; carbon emission and the heat value and heat efficiency of the solid product are predicted according to the simulation model, and absolute return of energy recovery economy and carbon emission of the system is established. Integrated analysis of process parameter optimization and energy recovery economy is achieved, a reliable digital tool is provided for process research, evaluation comprehensiveness is improved, laboratory and industrial application is more effectively connected, and efficient and energy-saving economic development of the repair technology is promoted.