89 results about "Liquid state fermentation" patented technology

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

The invention relates to a method for improving fermentation production efficiency of bacillus subtilis lipopeptide sodium by controlling threonine flow acceleration rate based on fermentation process oxygen uptake rate curve feedback. Bacillus subtilis is used as a production strain, and lipopeptide sodium is produced through deep liquid state fermentation. In the fermentation process, the oxygen uptake rate of the process is detected, a threonine and ammonium sulfate continuous feeding control process based on oxygen uptake rate curve parameters is implemented, the oxygen uptake rate in the fermentation process is stably maintained to be 60-65 mmo1 / L / h, the ammonium ion concentration is maintained to be 15-20 mmol / L, and rapid growth of hyphae and rapid increase of the lipopeptide sodium synthesis rate can be achieved. The threonine and ammonium sulfate fed-batch control process based on oxygen uptake rate feedback control is established, the production period is shortened, and the fermentation production level is stably improved.

The invention discloses saccharomyces cerevisiae XJ-YSSCE010-1 with high temperature resistance, high ethanol yield and low fusel oil yield and application of the saccharomyces cerevisiae XJ-YSSCE010-1, and belongs to the technical field of microbial fermentation. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention is separated and screened from Maotai-flavor liquor yeast, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31174. The fusel oil comprises the following main components: n-propanol, isobutanol, isoamyl alcohol, phenethyl alcohol and the like, wherein isobutanol and isoamyl alcohol have the highest toxicity. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention shows the advantages of high yield of ethanol, low yield of fusel oil and no production of isobutanol and isoamyl alcohol in liquid fermentation and simulated high-temperature stacking fermentation experiments, and the saccharomyces cerevisiae XJ-YSSCE010-1 is applied to preparation of white spirit, so that the production efficiency of the white spirit is improved, and the quality of the white spirit is improved.

The invention relates to the technical field of deep processing of agricultural and sideline products and feed additives, in particular to DDS pulp capable of degrading vomitoxin and glycerol and a preparation method of the DDS pulp. According to the method, the DDS pulp is inoculated with the specifically screened complex microbial inoculants, liquid fermentation is carried out under proper conditions, microorganisms preferentially utilize free glycerol in the DDS pulp as a carbon source for metabolism, the glycerol content is remarkably reduced in the process, and the palatability is fundamentally improved. According to the method, when the complex microbial inoculant is inoculated, the DDS pulp is inoculated with a functional strain with a vomitoxin degradation function and a special enzyme preparation, the functional strain is colonized in a system and continuously produces enzyme, and the added enzyme preparation can quickly and directly hydrolyze and destroy key toxicological groups in toxin molecules, so that the degradation efficiency of the vomitoxin is improved. And the degradation product is converted into a non-toxic or low-toxicity degradation product.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

The invention relates to a preparation method and application of ganoderma lucidum and coix seed mycoplasm, and belongs to the technical field of traditional Chinese medicine and health food processing. According to the preparation method of the lucid ganoderma and coix seed mycoplasm, the lucid ganoderma and coix seed mycoplasm is obtained through liquid fermentation by taking coix seeds as a fermentation substrate and lucid ganoderma as a fermentation strain. The ganoderma lucidum and coix seed mycoplasm composition is obtained through a ganoderma lucidum and coix seed two-way fermentation technology, functional ingredients are increased, and the ganoderma lucidum and coix seed mycoplasm composition has the effects of enhancing immunity, protecting pancreas islet beta cells and improving glycolipid metabolism. The invention develops the application of the traditional Chinese medicine composition in the fields of food and health care products.

The application discloses an engineering biogas fermentation device and a method thereof. The fermentation device comprises a liquid fermentation material feeding mechanism and a solid fermentation material feeding mechanism. The liquid fermentation material feeding mechanism comprises a homogenate pool, which is connected with a feeding port of the fermentation device through a liquid inlet pipe. The solid fermentation material feeding mechanism comprises a feeding hopper and a conveying device. The conveying device comprises a motor and a conveying belt driven by the motor. The feeding hopper is arranged on the conveying belt. The solid fermentation material enters the feeding hopper and is fed to the fermentation device by the conveying device as needed. The liquid fermentation material feeding mechanism and the solid fermentation material feeding mechanism cooperate to form a mixed area for feeding in two directions at the bottom of the fermentation device. The application can reduce the methane emission of fermentation materials in the storage link in the station and the energy efficiency loss caused by invalid degradation of the fermentation materials. The application can provide high-quality fermentation raw materials for a biogas project and realize effective quality preservation and emission reduction.

This invention discloses a method, system, equipment, and medium for activating and promoting the release of humic acid from silkworm excrement. The method includes: mechanochemical synergistic crystal-breaking pretreatment, i.e., dry ball milling to destroy the physical encapsulation structure and induce a local alkaline microenvironment under chemical solvent-free conditions; stepwise enzymatic hydrolysis bridging activation, utilizing the acid buffering properties of silkworm excrement, sequentially adding uricase, cellulase / hemicellulase, and laccase or manganese peroxidase to form a natural pH fluctuation curve; directed microbial induction and release promotion, inoculating with Bacillus amyloliquefaciens and Bacillus lentigines for liquid fermentation to achieve metal ion chelation and surface charge regulation; and finally, low-temperature drying and molding to obtain the product. This invention also discloses the system, computer equipment, and storage medium for implementing this method. This invention does not require the addition of external acids, alkalis, or chemical chelating agents, shortens the total cycle to 2-4 days, and the obtained product has a high water-soluble humic acid content, which enhances the resistance to refixation after application to the soil.

The invention relates to the technical field of dairy product processing, in particular to a yeast protein dairy product and a preparation method thereof.In the yeast protein preparation process, the quality of the yeast protein dairy product can be remarkably improved through the synergistic effect of key processes such as bi-enzyme directional step-by-step hydrolysis, ultrafiltration desalination concentration, compound bacteria liquid fermentation and an emulsification compound system; the yeast dairy product prepared by the method can be further suitable for products such as high-protein beverages, cheese substitutes and stirred yoghurt, and has the advantages of nutrition retention and texture optimization.

The invention relates to the technical field of fermentation, in particular to family farm liquid fermentation feeding equipment which comprises a mixing device and at least one fermentation device, and the mixing device is communicated with each fermentation device. Each fermentation device comprises a fermentation tank, a second stirring mechanism and a second control assembly, the fermentation tanks are communicated with the mixing tank, the second stirring mechanisms are arranged on the fermentation tanks and used for stirring materials entering the fermentation tanks, and the second control assemblies are arranged on the fermentation tanks and used for controlling the materials in the fermentation tanks. In the fermentation process, multi-stage stirring is adopted, the Reynolds number Re of the materials is larger than or equal to 2000 in the fermentation process, the uniformity of the materials is improved, the number of viable bacteria of the materials is increased, and therefore the feeding error rate is reduced.

The invention discloses an automatic mixed fermentation system and process for preparing a traditional Chinese medicine multi-strain fermented beverage, and relates to the technical field of beverage production and fermentation. According to the automatic mixed fermentation system and process for preparing the traditional Chinese medicine multi-strain fermented beverage, through cooperation of the mixing frame mechanism, the driving mechanism, the cam frame mechanism, the gas pumping and discharging mechanism and the solution pumping and discharging mechanism, the driving mechanism can drive the mixing frame mechanism to rotate, liquid traditional Chinese medicine and various probiotics in the fermentation barrel are stirred and mixed, and the liquid traditional Chinese medicine and the various probiotics are mixed; the gas pumping and discharging mechanism and the solution pumping and discharging mechanism can also be driven to work, so that the gas pumping and discharging mechanism pumps external gas into the fermentation barrel, the dissolved oxygen amount in materials in the fermentation barrel is controlled, the dissolved oxygen requirement in the liquid state fermentation process is met, the solution pumping and discharging mechanism pumps external acid-base adjusting liquid into the fermentation barrel, and the fermentation efficiency is improved. The pH value of the material is maintained in a required range, the large fluctuation of the dissolved oxygen amount and the pH value caused by microbial metabolism in the fermentation process is prevented, and stable liquid fermentation is guaranteed.

The invention discloses a method for increasing the yield of monascus pigment through liquid fermentation, the monascus pigment is obtained through spore culture, seed culture, liquid fermentation and monascus pigment extraction by taking monascus as a production strain, and macroporous resin is added into a fermentation culture medium. According to the method, the yield of liquid fermentation of the monascus pigment can be greatly increased, the monascus pigment is concentrated in precipitates, namely resin and hyphae after solid-liquid separation is carried out on the fermentation product, a water phase of fermentation liquor does not contain pigment, separation and extraction of downstream fermentation products and treatment of fermentation waste liquor are facilitated, and the method has good market prospects and market value.

The application discloses an application of Armillaria mellea in degrading vomitoxin, and further discloses the Armillaria mellea Am-07-22 with a preservation number of CCTCC NO: M 20221044; the mycotoxin is vomitoxin or zearalenone. The strain Am-07-22 has the best degrading effect when the concentration of vomitoxin is 2 mu g / mL, and the degrading rate is 64.05%; the Armillaria mellea has the degrading effect on vomitoxin in two different fermentation raw materials, the degrading effect is good when adopting a solid-state fermentation mode for corn husks, and the degrading effect is good when adopting a liquid-state fermentation mode for corn yellow powder.

The present disclosure relates to a process for capturing CO2 using bio-digester slurry, along with a method to improve the quality of fermented organic manure (FOM) and liquid fermented organic liquor (LFOM). The present disclosure describes the use of lignin functionalizing microbes (LFM) and H2S oxidizing microbes (SOD) to enhance the quality of the FOM and LFOM and improve the CO2 capture ability of the slurry. The CO2 and / or H2S captured manure increases their nutrient value for use in agriculture. The CO2 absorption process not only aids in reducing the amount of greenhouse gases released into the environment, but also improves the quality of FOM and LFOM. The integration of these processes results in a sustainable and efficient approach for capturing CO2 while improving the quality of organic waste material for agricultural use.

The present application relates to a kind of for sensitive skin improves mood stress skin aging composition preparation and application, belong to cosmetic technical field.The raw material of the composition for sensitive skin improves mood stress skin aging provided in the present application includes mulberry root extract, figwort flower extract, with distance stone branch algae extract, palmitoyl tetrapeptide-7, wherein with distance stone branch algae extract is prepared by bidirectional liquid fermentation method, four ingredients scientific compound, form " source control cortisol-skin structure repair " complete path, through target point clear, ring interlocking synergies, it is realized to sensitive skin mood stress skin aging Systematic improvement, provides a kind of mild, efficient and mechanism clear solution for sensitive skin anti-aging.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The invention discloses a preparation process of a composition based on corn straw, corn stigma and grain juice, which comprises the following steps: solid state fermentation, liquid state fermentation, low temperature distillation, blending and filling to obtain the grain juice composition, the formaldehyde content of the grain juice composition is lower than 0.5 mg / L, and the grain juice composition is rich in corn peptide. According to the composition based on the corn straw, the corn stigma and the grain juice and the preparation process of the composition, the corn straw and the corn stigma are subjected to optimized solid-state and liquid-state fermentation respectively, and a specific low-temperature distillation technology is combined, so that efficient and mild extraction and conversion of nutritional ingredients and active substances in the raw materials are realized; according to the process, the content of bad byproducts such as formaldehyde in a final product is remarkably reduced, and meanwhile, rich natural active ingredients derived from raw materials are reserved to the greatest extent.

The invention discloses expansin recombinant aspergillus niger suitable for liquid fermentation as well as a construction method and application thereof, and belongs to the technical field of recombinant microorganisms. The technical problem of efficiently degrading cellulose is solved. The invention provides a recombinant aspergillus niger, and the recombinant aspergillus niger is obtained by taking aspergillus niger TH-2-D as a starting strain and replacing a saccharifying enzyme gene glaA as shown in SEQ ID NO.31 with a penicillium oxalicum expansin Swo gene as shown in SEQ ID NO.28. The invention also provides a preparation method of the recombinant aspergillus niger. The strain is subjected to liquid state fermentation, fermentation liquor is suitable for fiber puffing application, the cellulose degradation efficiency can be improved when the strain is used in cooperation with cellulase, linked starch can be effectively removed when the strain is used in cooperation with corn wet grinding enzyme, and the starch yield is increased.

The application discloses a kind of enzyme processing combination liquid fermentation production brewing type tobacco flavor agent's method, comprising the following steps: S1, cut tobacco is mixed with sorghum powder, after adding distilled water obtains tobacco-sorghum liquid medium;S2, yeast strain is inoculated to YPD culture medium and is cultivated, obtains yeast bacterial liquid;And / or lactobacillus strain is inoculated to MRS culture medium and is cultivated, obtains lactobacillus bacterial liquid;S3, configuration enzyme preparation solution, enzyme includes one or more of amylase, flavourzyme and saccharifying enzyme;S4, enzyme preparation solution is added to tobacco-sorghum liquid culture medium;S5, the yeast bacterial liquid and / or lactobacillus bacterial liquid of S2 is inoculated to the culture medium of S4 and is cultivated, obtains fermentation liquid flavor medium;S6, fermentation liquid flavor medium is freeze-dried, obtains tobacco flavor agent.The brewing type flavor agent of the application is prepared based on microbial fermentation technology, process is simple, condition is mild, cost is low, green environmental protection, improve the economic benefit of low quality cigarette.

The present application relates to the technical field of probiotic composition, and discloses a bacterial agent for regulating vaginal microbial flora and a preparation method thereof, which comprises the following steps: preparing Lactobacillus paracasei KFY202407 powder into a seed solution; performing segmented fermentation culture on the seed solution; obtaining bacterial slurry after centrifugal enrichment of the fermentation product; mixing the bacterial slurry with a composite freeze-drying protective agent and then performing freeze-drying to obtain freeze-dried bacterial powder; and performing gelatinization treatment on the freeze-dried bacterial powder to obtain the bacterial agent. The present application realizes rapid proliferation of bacterial cells and promotes accumulation of active metabolites by adopting a two-stage fermentation process of liquid-state fermentation followed by solid-state fermentation, thereby solving the technical problems of low active bacterial proliferation efficiency and insufficient metabolites in the prior art. Meanwhile, the present application adopts a freeze-drying process of two-stage pre-freezing and drying sublimation, so that ice crystals are removed in the form of sublimation instead of melting, thereby solving the technical problem of bacterial cell damage and uncontrollable inactivation due to ice crystals in the traditional freeze-drying technology.

The present application relates to a kind of tobacco flavor preparation method and its product and application, comprising the following steps: tobacco, sorghum and water are mixed to be sterilized, obtain tobacco-sorghum liquid medium, enzyme preparation and bacteria liquid are added to tobacco-sorghum liquid medium and are cultured fermentation, the bacteria liquid is yeast bacteria liquid and / or lactobacillus bacteria liquid, obtain the tobacco flavor.The present application uses sorghum and tobacco as common fermentation substrate, gives the double flavor of final flavoring liquor and tobacco flavor, using enzyme treatment combined with liquid fermentation method, solve the problem that the flavor brought by traditional stacking solid fermentation often needs to be passed through long time and flavor is insufficient etc., process is simple, condition is mild, green and environmental protection, cost is low, significantly improve the flavor quality of low quality cigar, improve the economic value of low quality cigar, with wide application prospect.

The invention belongs to the field of microorganisms, and particularly relates to a construction method and application of genetically engineered bacteria for super-high yield of monascus pigment. The invention discloses a construction method and application of a genetically engineered bacterium with superhigh yield of monascus pigment. The strain has the characteristic of superhigh yield of monascus pigment, and is obtained by taking red monascus as an original strain and screening by knocking out a gene MpSte11A. The invention discloses an application of the genetically engineered bacterium in microbial fermentation preparation of monascus pigment, and a liquid fermentation result shows that the highest intracellular total pigment content of MpSte11A on the 15th day is 112U / mL, which is 22 times of the yield (5.45 U / mL) of an original strain. The recombinant strain obviously promotes the production of monascus pigment under a liquid state fermentation condition, improves the fermentation efficiency, and has good commercial and scientific research values.

This invention relates to a processing device for preparing feed by fermenting corn cobs. Belonging to the field of fermentation preparation, the device includes a fermentation cylinder, a top baffle, and a separation component. A temperature control tube is located at the center of the side of the fermentation cylinder. The top baffle is positioned above the fermentation cylinder, and the separation component is disposed within the fermentation cylinder. The separation component includes a movable shaft, a movable frame, a curved frame, and a linkage sleeve. The movable shaft is positioned behind the top baffle during its rotational movement, and a motor is mounted on the top baffle. This invention uses a curved frame driven by the movable shaft to dynamically separate the inner wall of the fermentation cylinder. Simultaneously, a sealed telescopic frame guides the liquid fermentation medium to the inner wall in two directions via a guide passage, achieving both wetting and efficient separation of adhering corn cobs and auxiliary materials. This effectively prevents material solidification and clumping, significantly improving the utilization rate of corn cobs and overcoming the wall-hanging loss problem commonly found in existing fermentation equipment.

The application discloses a method for preparing functional caragana fiber compound feed and belongs to the technical field of feed processing. The method comprises the following steps: taking caragana as raw material, performing solid-liquid separation after dilute sulfuric acid pretreatment, preparing cordycepin and lysine through liquid state fermentation of cordyceps militaris and glutamic acid corynebacterium respectively, neutralizing caragana solid fiber and caragana fiber raw material obtained through ammonia treatment, and preparing fiber feed through solid anaerobic fermentation of the compound fermentation strain, and finally, compounding the three to obtain functional caragana fiber compound feed. The method realizes efficient utilization of all components of caragana sugar and fiber resources, and the prepared compound feed is rich in cordycepin, lysine and dietary fiber, the crude protein content of the fiber feed is more than 20%, and the fiber feed is rich in cordycepin and lysine functional components. The method not only provides a new way for resource utilization and high value utilization of caragana, but also provides a new idea for research and development of functional coarse feed, and has good economic, ecological and industrial popularization value.

The invention relates to salvia miltiorrhiza and poria cocos combined fermentation liquor and application research thereof in cosmetics, and aims to discuss the reliability of the salvia miltiorrhiza and poria cocos combined fermentation liquor as a functional raw material of the cosmetics. The HYJ bacteria are used for carrying out liquid state fermentation on the salvia miltiorrhiza and poria cocos combination, and the content change of key effective components in the fermentation liquid is researched. A high performance liquid chromatography method is adopted for quantitative analysis, and the anti-oxidation, anti-allergy and whitening effects of the fermentation liquor are evaluated. The research shows that the salvia miltiorrhiza and poria cocos combined fermentation liquor shows higher oxidation resistance than that of an unfermented group in a free radical scavenging experiment of 1, 1-diphenyl-2-trinitrophenylhydrazine and 2, 2-nitrogen-bis (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt; meanwhile, the inhibition effect of the fermentation liquor on hyaluronidase and tyrosinase is obviously enhanced compared with that of an unfermented group, so that the fermentation liquor has good anti-allergic and whitening effects. The research provides an experimental basis for the application of the salvia miltiorrhiza and poria cocos combined fermentation liquor in cosmetics, and proves the potential of the salvia miltiorrhiza and poria cocos combined fermentation liquor as a functional raw material of the cosmetics.

The invention provides a preparation method of a microbial fermentation five-flavor mannan medicated bath lotion, and belongs to the technical field of fermentation processes. The preparation method comprises the following steps: crushing Wuwei ganlu medicated bath raw materials, and carrying out wall breaking treatment through steam explosion to obtain a steam explosion substrate; adjusting the water content of the steam explosion substrate, inoculating enzyme-producing fungi to carry out aerobic solid-state fermentation, and carrying out enzymolysis on the substrate by utilizing an extracellular enzyme system secreted by the fungi to obtain a solid-state fermentation product; adding water into the solid-state fermentation product, inoculating transformed bacteria and saccharomycetes, carrying out anaerobic liquid-state fermentation, forming a water-alcohol-acid mixed system through microbial metabolism, and transforming and extracting active components to obtain fermented mash; and carrying out solid-liquid separation on the fermented mash, and carrying out membrane separation, filtration, sterilization and impurity removal on the obtained liquid to obtain the microbial fermentation five-flavor mannan medicated bath liquid. Through steam explosion physical modification and solid-liquid composite fermentation biotransformation, effective dissociation of medicinal material cell walls and improvement of substrate utilization rate are realized.

The invention provides a preparation method of white tea enzyme liquid, and belongs to the technical field of food biology.The method comprises the following steps of raw material pretreatment, preparation of dry white tea and purified water, preparation of Chinese patent medicine compound distiller's yeast, liquid fermentation of the compound distiller's yeast, distillation and separation of tea liquor, and refining and sterilization of the white tea enzyme liquid. According to the invention, 24 Chinese patent medicines with homology of medicine and food are blended into the distiller's yeast for preparation, so that the enzyme liquid has the composite health-care functions of oxidation resistance (tea polyphenol), bioflavonoids and the like of white tea at the same time, and the core pain point of single effect of the existing product is solved.

The invention discloses an organic fertilizer for white ginger planting and a preparation method thereof, and belongs to the technical field of agricultural fertilizers. The organic fertilizer is composed of a basic fermentation material and a microbial functional complex. The microbial functional complex is prepared by performing liquid state fermentation on a complex microbial inoculant consisting of bacillus subtilis, trichoderma harzianum and photosynthetic bacteria and leftovers of Chinese herbal medicines such as liquorice, astragalus membranaceus and the like to obtain functional fermentation liquor, and loading the functional fermentation liquor on porous biochar. Through the synergistic effect of the composite flora, the Chinese herbal medicine fermentation product and the charcoal carrier, a'microbial antagonism-plant immune induction 'disease prevention and control system is constructed, and soil-borne diseases such as ginger blast and the like can be efficiently inhibited; the content of gingerol in the white ginger can be remarkably increased by a Chinese herbal medicine fermentation product; meanwhile, the biochar can effectively adsorb and passivate heavy metals in the soil, and the enrichment of the heavy metals in the ginger blocks is reduced. The problems that in the prior art, organic fertilizer is poor in disease prevention effect, the quality cannot be improved, and the heavy metal pollution risk cannot be solved are solved.

The invention relates to the technical field of peach wine production, in particular to peach and multi-grain primary pulp co-fermented liquor and a preparation method thereof.The preparation method comprises the following steps that multi-grain primary pulp liquor is prepared, specifically, sorghum primary pulp liquor, wheat primary pulp liquor and corn primary pulp liquor are produced through a solid-state fermentation method, and rice primary pulp liquor and sticky rice primary pulp liquor are produced through a liquid-state fermentation method; the preparation method comprises the following steps: selecting 90-percent ripe juicy peach fruits, performing pretreatment of impurity removal, cleaning, sterilization by soaking in a disinfectant, secondary cleaning and secondary high-temperature sterilization, removing kernels, pulping, and cooling to obtain peach pulp; mixing the peach pulp with the multi-grain original pulp wine, adding sugar and distiller's yeast for fermentation, and obtaining peach pulp mash after fermentation is finished; and distilling the peach pulp mash, performing head-cutting and tail-removing operation, and performing classified storage to obtain the finished product peach white wine, thereby achieving the technical effects of realizing deep association of fruit and grain flavors, enabling the wine body to be soft, mellow and harmonious, blocking the generation of harmful substances from the source, and remarkably improving the fermentation stability and the safety of the finished product.

The invention discloses a brewing process of liquid fermented wine rich in gamma-aminobutyric acid, which comprises solid saccharification and inoculation of corynebacterium glutamicum for fermentation and liquid inoculation of Lactobacillus hilgardii for fermentation, the solid saccharification and inoculation of corynebacterium glutamicum for fermentation can generate glutamic acid in the saccharification process, and the liquid fermented wine rich in gamma-aminobutyric acid is obtained by adding water and inoculation of Lactobacillus hilgardii for liquid fermentation. Glutamic acid is metabolized to generate gamma-aminobutyric acid, and a solid saccharification inoculation corynebacterium glutamicum fermentation process and a liquid inoculation lactobacillus hirsuti fermentation process do not influence the consumption of wine production substances, so that the content of gamma-aminobutyric acid in the fermented wine is increased under the conditions of not influencing the alcoholic strength of the fermented wine and not changing the flavor sense of the raw wine.