15 results about "Liquid state fermentation" patented technology

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

The application discloses an engineering biogas fermentation device and a method thereof. The fermentation device comprises a liquid fermentation material feeding mechanism and a solid fermentation material feeding mechanism. The liquid fermentation material feeding mechanism comprises a homogenate pool, which is connected with a feeding port of the fermentation device through a liquid inlet pipe. The solid fermentation material feeding mechanism comprises a feeding hopper and a conveying device. The conveying device comprises a motor and a conveying belt driven by the motor. The feeding hopper is arranged on the conveying belt. The solid fermentation material enters the feeding hopper and is fed to the fermentation device by the conveying device as needed. The liquid fermentation material feeding mechanism and the solid fermentation material feeding mechanism cooperate to form a mixed area for feeding in two directions at the bottom of the fermentation device. The application can reduce the methane emission of fermentation materials in the storage link in the station and the energy efficiency loss caused by invalid degradation of the fermentation materials. The application can provide high-quality fermentation raw materials for a biogas project and realize effective quality preservation and emission reduction.

The present application relates to the technical field of probiotic composition, and discloses a bacterial agent for regulating vaginal microbial flora and a preparation method thereof, which comprises the following steps: preparing Lactobacillus paracasei KFY202407 powder into a seed solution; performing segmented fermentation culture on the seed solution; obtaining bacterial slurry after centrifugal enrichment of the fermentation product; mixing the bacterial slurry with a composite freeze-drying protective agent and then performing freeze-drying to obtain freeze-dried bacterial powder; and performing gelatinization treatment on the freeze-dried bacterial powder to obtain the bacterial agent. The present application realizes rapid proliferation of bacterial cells and promotes accumulation of active metabolites by adopting a two-stage fermentation process of liquid-state fermentation followed by solid-state fermentation, thereby solving the technical problems of low active bacterial proliferation efficiency and insufficient metabolites in the prior art. Meanwhile, the present application adopts a freeze-drying process of two-stage pre-freezing and drying sublimation, so that ice crystals are removed in the form of sublimation instead of melting, thereby solving the technical problem of bacterial cell damage and uncontrollable inactivation due to ice crystals in the traditional freeze-drying technology.

This invention relates to a processing device for preparing feed by fermenting corn cobs. Belonging to the field of fermentation preparation, the device includes a fermentation cylinder, a top baffle, and a separation component. A temperature control tube is located at the center of the side of the fermentation cylinder. The top baffle is positioned above the fermentation cylinder, and the separation component is disposed within the fermentation cylinder. The separation component includes a movable shaft, a movable frame, a curved frame, and a linkage sleeve. The movable shaft is positioned behind the top baffle during its rotational movement, and a motor is mounted on the top baffle. This invention uses a curved frame driven by the movable shaft to dynamically separate the inner wall of the fermentation cylinder. Simultaneously, a sealed telescopic frame guides the liquid fermentation medium to the inner wall in two directions via a guide passage, achieving both wetting and efficient separation of adhering corn cobs and auxiliary materials. This effectively prevents material solidification and clumping, significantly improving the utilization rate of corn cobs and overcoming the wall-hanging loss problem commonly found in existing fermentation equipment.

The application discloses a method for preparing functional caragana fiber compound feed and belongs to the technical field of feed processing. The method comprises the following steps: taking caragana as raw material, performing solid-liquid separation after dilute sulfuric acid pretreatment, preparing cordycepin and lysine through liquid state fermentation of cordyceps militaris and glutamic acid corynebacterium respectively, neutralizing caragana solid fiber and caragana fiber raw material obtained through ammonia treatment, and preparing fiber feed through solid anaerobic fermentation of the compound fermentation strain, and finally, compounding the three to obtain functional caragana fiber compound feed. The method realizes efficient utilization of all components of caragana sugar and fiber resources, and the prepared compound feed is rich in cordycepin, lysine and dietary fiber, the crude protein content of the fiber feed is more than 20%, and the fiber feed is rich in cordycepin and lysine functional components. The method not only provides a new way for resource utilization and high value utilization of caragana, but also provides a new idea for research and development of functional coarse feed, and has good economic, ecological and industrial popularization value.

This invention discloses a method for increasing the yield of arachidonic acid (RAA) produced by *Morchella spp.* fermentation, relating to microbial fermentation technology. The method employs either solid-state fermentation or liquid-state shallow static fermentation. *Morchella spp.*, used for RAA synthesis, is inoculated onto the surface of a solid-state fermentation medium for solid-state fermentation, or inoculated into a liquid fermentation medium for liquid-state shallow static fermentation. Feeding is performed during fermentation until the RAA content in the *Morchella spp.* reaches its peak. This method can increase the RAA content in synthesized oils to 1.72 times that of deep fermentation, reaching a maximum of 80.93%. The yield of RAA synthesized by *Morchella spp.* per 1L of culture medium consumed is up to four times that of deep fermentation. This method requires less equipment investment, is simple to operate, has very low energy consumption, is pollution-free, and allows for large-scale production of RA-rich oils and microorganisms, showing broad application prospects in pharmaceutical intermediates, nutritional supplements, and animal feed.

This utility model discloses a multi-layer stirred liquid fermenter, including a stirred tank and a stirring unit. The stirred tank has at least three fermentation chambers, each with a fixed sleeve. The stirring unit is located inside the stirred tank and has a vertically rotating shaft inside. Stirring blades (a) are arranged on the side of the rotating shaft. A rotating ring is nested outside the rotating shaft, and stirring blades (b) are arranged on the side of the rotating ring. A conical tooth (b) is vertically rotating inside the fixed sleeve. The conical tooth (b) meshes with the rotating shaft and the rotating ring. By incorporating the stirring unit, this utility model allows the rotating shaft to rotate via a motor, causing the conical tooth (b) to rotate. The conical tooth (b) then drives the rotating ring to rotate in the opposite direction, thereby creating a counter-stirring effect through the stirring blades (a and b), improving the mixing uniformity and reaction efficiency of the fermentation broth.

The present application belongs to the technical field of alternative immunomodulators, and particularly relates to a preparation method of a Passiflora edulis immunomodulator for pigs and application thereof, which comprises the following steps: inoculating activated gold flower fungus into a liquid culture medium to prepare seed liquid; pretreating Passiflora edulis fruits, cutting or crushing the Passiflora edulis fruits to prepare a fermentation substrate, and adding bran and / or corn powder as an auxiliary carbon source; sterilizing the fermentation substrate; inoculating the seed liquid to perform solid or liquid fermentation; in the fermentation process, the gold flower fungus secretes an enzyme system to destroy the cell wall of the Passiflora edulis, release and convert polysaccharides and flavonoid active ingredients; and drying and crushing to obtain the Passiflora edulis immunomodulator for pigs. The immunomodulator can be added into pig feed to significantly improve the spleen index, macrophage phagocytic activity, lymphocyte proliferation capacity and serum IgG level, reduce the diarrhea rate, and promote growth performance. The present application combines the gold flower fungus and the Passiflora edulis to prepare the immunomodulator for pigs, which can be used to replace antibiotics and promote healthy pig breeding.

This invention relates to the field of microbial fermentation feed technology, and discloses a zero-wastewater feed ingredient production method based on two-stage fermentation. The method includes inoculating *Bacillus bellis* into a liquid fermentation medium for liquid fermentation to obtain a liquid fermentation mixture; centrifuging all of the liquid fermentation mixture to obtain *Bacillus bellis* cells and collecting the supernatant containing the remaining *Bacillus bellis*; adding yeast and lactic acid bacteria to the collected supernatant, then mixing it with solid fermentation raw materials to form a solid-liquid mixture; adding purified water or solid fermentation raw materials to control the fermentation moisture content; and then fermenting the moisture-adjusted solid-liquid mixture under sealed conditions to obtain a pre-fermented mixture as the feed ingredient. This invention avoids wastewater generation during the production process and improves the digestive enzyme activity of the product.

The utility model relates to quantitative feeding equipment field especially relates to fermentation carbon source quantitative feeding device, including mixed jar body, still including feeding cylinder, the upper end fixed mounting of mixed jar body has drive motor, the output end of drive motor extends to the inside fixed mounting of mixed jar body has transmission shaft, the surface fixed mounting of transmission shaft has stirring rod, the lower end fixed connection of mixed jar body has the feed pipe, the lower end fixed mounting of feeding cylinder has feeding pipe, the utility model discloses drive stirring rod with drive motor and mix and stir, thereby process the liquid fermentation carbon source of need, cooperate feeding cylinder and carry out quantitative feeding to liquid fermentation carbon source, through mixed structure and convert solid fermentation carbon source or multiple carbon source into mixed liquid carbon source, further convenient flow to the quantitative measurement work of fermentation carbon source, and liquid fermentation carbon source is more easy to disperse, and the more uniform of feeding, compared with traditional carbon source, the liquid compound carbon source can save the dosage of use, improve fermentation efficiency and product yield.

The application discloses a kind of liquid fermentation and liquid feeding method for pickled mustard tail, belong to fermentation feed and feeding technical field, and fermentation mechanism is equipped with adjustable feeding mechanism;Through stirring assembly, the stirring operation of fermentation material is realized, and using stirring as power source, tail and concentrate can be driven into stirring assembly by material conveying assembly, and through material conveying assembly, up and down convection of fermentation material in stirring box can also be realized, which breaks the stratification of fermentation material, improves the mixing uniformity and efficiency, and after stirring is completed, the discharge direction is adjusted by discharge assembly, and the fermentation material can be directly discharged into fermentation barrel, then lactobacillus plantarum is connected, and liquid fermentation is carried out at 20-35 DEG C. After fermentation, stable quality liquid fermented feed is obtained. After fermentation is completed, the material conveying assembly can also be used to take out and put into the liquid feeding system, to realize the integration of the whole process of feeding, mixing and fermentation, to make the whole process more simple and convenient, and to improve the work efficiency.

The application discloses a method for preparing white pepper from black pepper by using Saccharomyces cerevisiae Y15. The strain has been preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on January 29, 2026, and the preservation number is GDMCC NO: 67767, and the taxonomic name is Saccharomyces cerevisiae. The method inoculates the Saccharomyces cerevisiae into the black pepper to perform liquid fermentation, and the fermentation process is optimized by regulating the mechanical peeling time and degree, the fermentation temperature and the amount of bacterial liquid, so that the white pepper is efficiently prepared. The core of the application lies in the combination of mechanical peeling and inoculation of Saccharomyces cerevisiae fermentation, which not only effectively improves the peeling efficiency of the black pepper, but also makes the obtained white pepper particles complete and the aroma pure, and makes the fermentation process controllable and edible safer.

The application discloses a biocontrol agent, a preparation method and application of the biocontrol agent in prevention and treatment of root rot of Salvia miltiorrhiza, and belongs to the technical field of microbial materials. The biocontrol agent comprises a solid bacterial agent, a liquid bacterial agent and a microbial activity promoter; the solid bacterial agent is prepared by solid-state fermentation of Trichoderma harzianum CGMCC 3.10154; the liquid bacterial agent is prepared by liquid-state fermentation of Bacillus lentimorbus CGMCC 1.9055; and the microbial activity promoter is composed of L-glutamine, mannitol and sodium molybdate in a mass ratio of (1-3):(2-5):(0.2-0.5). The bacterial agent of the application effectively solves the problems of weak colonization ability and unstable prevention effect of the existing biocontrol agent through the synergistic effect of functional strains and the promoter, significantly promotes the growth of Salvia miltiorrhiza, improves the yield and the content of active ingredients such as salvianolic acid B, realizes the comprehensive effects of disease prevention, growth promotion and quality improvement, and has a wide application prospect.

This invention discloses a method for preparing liquid fermented tea that enhances the floral and fruity aroma of tea leaves, belonging to the field of fermented tea technology. This invention uses Pichia pastoris (a type of yeast). Pichia cactophila FL2 fermented tea liquor, after fermentation, shows an increase of more than 11.59 times in the content of floral and fruity aroma compounds such as terpinene, an increase of 86.58 times in the content of phenylethanol, an increase of 82.83 times in the content of phenethyl acetate, and an increase of 369.26 μg / mL in the content of 1-butanol and o-cymene from 0. In addition, the increases in cymene, α-terpineol, and nerolidol reached 5.41, 13.54, and 3.34 times, respectively. The interaction of various aroma components gives the tea liquor a unique "lemon and citrus fruity aroma" flavor, while also containing sweet and sour aroma compounds. It has a good flavor and is of industrial promotion value.

This invention discloses a method for enzymatic pretreatment of wet hydrolysate from kitchen waste. The method involves wet hydrolyzing the kitchen waste slurry and separating the three phases, with the liquid phase product being the wet hydrolysate. A portion of the wet hydrolysate is fed into a liquid fermentation tank, where an enzyme preparation is added for liquid fermentation to obtain a composite enzyme solution. The composite enzyme solution is then mixed with another portion of the wet hydrolysate for enzymatic hydrolysis, completing the pretreatment. This invention also discloses a method for treating kitchen waste, first using the aforementioned enzymatic pretreatment method for the wet hydrolysate, and then feeding the enzymatically hydrolysate into a high-efficiency anaerobic reactor for anaerobic treatment. This invention can reduce the investment cost of enzymatic pretreatment and even kitchen waste treatment without affecting the treatment efficiency.