127 results about "Liquid state fermentation" patented technology

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

The invention relates to the technical field of sewage biochemical treatment, and discloses a preparation method of an efficient composite biological carbon source, which comprises the following steps: solid-liquid two-phase fermentation treatment, microbial community echelon relay treatment, fermentation liquor reflux and biological detoxification system and biomass enhancement treatment. According to the invention, the preparation of the composite biological carbon source with high concentration (COD > = 50,000 mg / L), high stability (stable operation time > = 120 days) and high activity is realized by combining solid state fermentation and liquid state fermentation, constructing a microbial echelon metabolic network, applying a porous carrier layer to eliminate an inhibitor, implementing active factor regeneration and other technical means; according to the present invention, the organic matter conversion efficiency is improved by more than 30%, the stable operation time of the system is improved, the technical problems of insufficient concentration, unbalanced components, poor stability and the like in the traditional carbon source preparation are solved, the method is suitable for the nitrogen and phosphorus removal process of the large-scale sewage treatment plant, the total nitrogen removal rate of the sewage treatment system can be improved by 15-25%, and the nitrogen removal rate can be improved by 30-40%.

The invention relates to a method for improving fermentation production efficiency of bacillus subtilis lipopeptide sodium by controlling threonine flow acceleration rate based on fermentation process oxygen uptake rate curve feedback. Bacillus subtilis is used as a production strain, and lipopeptide sodium is produced through deep liquid state fermentation. In the fermentation process, the oxygen uptake rate of the process is detected, a threonine and ammonium sulfate continuous feeding control process based on oxygen uptake rate curve parameters is implemented, the oxygen uptake rate in the fermentation process is stably maintained to be 60-65 mmo1 / L / h, the ammonium ion concentration is maintained to be 15-20 mmol / L, and rapid growth of hyphae and rapid increase of the lipopeptide sodium synthesis rate can be achieved. The threonine and ammonium sulfate fed-batch control process based on oxygen uptake rate feedback control is established, the production period is shortened, and the fermentation production level is stably improved.

The invention provides a complex microbial inoculant for fermenting modified corn flour, a method and application, and belongs to the technical field of fermented food. The complex microbial inoculant comprises a liquid state fermentation complex microbial inoculant (streptococcus thermophilus is greater than or equal to 1 * 10 < 9 > cfu / mL and bacillus subtilis is greater than or equal to 1 * 10 < 9 > cfu / mL) and a solid state fermentation complex microbial inoculant (monascus spore suspension is greater than or equal to 1 * 10 < 6 > spores / mL and lactobacillus plantarum is greater than or equal to 1 * 10 < 8 > cfu / mL), and the fermentation modification method comprises the following steps: performing microwave puffing treatment on corn flour, mixing the corn flour with oat bran, and sequentially performing liquid state fermentation and solid state fermentation, and spraying the liquid fermentation supernatant back to the solid substrate, and finally drying to obtain the modified corn flour. Through the synergistic effect of a two-stage fermentation system, the content of functional components such as soluble dietary fibers and Monacolin K in the corn flour is remarkably increased, and the obtained product has the characteristic of lowering cholesterol.

The invention relates to a protein feed prepared from biologically refined distiller's grains and a preparation method thereof, and relates to the technical field of bioengineering and feed resource development. The method comprises the following steps: (1) sequentially carrying out acid pretreatment and double-enzyme synergistic hydrolysis on the white spirit vinasse to obtain hydrolysate containing fermentable sugar; (2) adding a candida utilis yeast liquid and urea, and carrying out carbon-nitrogen synergistic liquid fermentation to obtain a fermentation liquid with the viable count of candida utilis greater than or equal to 4.0 * 10 < 8 > CFU / mL; and drying. According to the method, the white spirit vinasse is subjected to diluted acid high-pressure pretreatment and double-enzyme synergistic hydrolysis, then candida utilis is utilized for carbon-nitrogen synergistic liquid fermentation, efficient utilization of glucose and xylose is achieved, and the mycoprotein yield is larger than or equal to 0.70 g / L / h; according to the method, all-component high-value utilization of the distiller's grains is realized, and a process path with industrial production potential is provided for preparing the protein feed by biorefining the distiller's grains.

This invention relates to a protein feed prepared from biorefined baijiu lees and its preparation method, belonging to the fields of bioengineering and feed resource development technology. It includes the following steps: (1) subjecting the baijiu lees to acid pretreatment and dual-enzyme synergistic hydrolysis sequentially to obtain a hydrolysate containing fermentable sugars; (2) adding Candida utilis culture and urea for carbon-nitrogen synergistic liquid fermentation to obtain a Candida utilis viable count ≥4.0 × 10⁻⁶. 8 Fermentation broth with CFU / mL; drying is then performed. This invention pretreats baijiu (Chinese liquor) lees under high pressure with dilute acid, followed by synergistic hydrolysis with two enzymes, and then utilizes *Candida utilis* for carbon-nitrogen synergistic liquid fermentation, achieving efficient utilization of glucose and xylose, with a cell protein yield ≥0.70 g / L / h. This invention realizes the high-value utilization of all components of baijiu lees, providing a process route with industrial-scale production potential for the biorefining of baijiu lees to prepare protein feed.

The invention discloses saccharomyces cerevisiae XJ-YSSCE010-1 with high temperature resistance, high ethanol yield and low fusel oil yield and application of the saccharomyces cerevisiae XJ-YSSCE010-1, and belongs to the technical field of microbial fermentation. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention is separated and screened from Maotai-flavor liquor yeast, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31174. The fusel oil comprises the following main components: n-propanol, isobutanol, isoamyl alcohol, phenethyl alcohol and the like, wherein isobutanol and isoamyl alcohol have the highest toxicity. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention shows the advantages of high yield of ethanol, low yield of fusel oil and no production of isobutanol and isoamyl alcohol in liquid fermentation and simulated high-temperature stacking fermentation experiments, and the saccharomyces cerevisiae XJ-YSSCE010-1 is applied to preparation of white spirit, so that the production efficiency of the white spirit is improved, and the quality of the white spirit is improved.

The invention relates to the technical field of deep processing of agricultural and sideline products and feed additives, in particular to DDS pulp capable of degrading vomitoxin and glycerol and a preparation method of the DDS pulp. According to the method, the DDS pulp is inoculated with the specifically screened complex microbial inoculants, liquid fermentation is carried out under proper conditions, microorganisms preferentially utilize free glycerol in the DDS pulp as a carbon source for metabolism, the glycerol content is remarkably reduced in the process, and the palatability is fundamentally improved. According to the method, when the complex microbial inoculant is inoculated, the DDS pulp is inoculated with a functional strain with a vomitoxin degradation function and a special enzyme preparation, the functional strain is colonized in a system and continuously produces enzyme, and the added enzyme preparation can quickly and directly hydrolyze and destroy key toxicological groups in toxin molecules, so that the degradation efficiency of the vomitoxin is improved. And the degradation product is converted into a non-toxic or low-toxicity degradation product.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

The application belongs to the technical field of medicine, and particularly relates to Phellinus orientoasiaticus and application thereof. Phellinus orientoasiaticus (strain IFPW11189) is preserved in the China General Microbiological Culture Collection Center, and the preservation date is August 15, 2024, and the preservation number is CGMCC No.41436. The application can improve the flavone yield of liquid-state fermentation of Phellinus orientoasiaticus, and provides scientific guidance for the development of medicinal fungal resources.

The invention relates to a preparation method and application of ganoderma lucidum and coix seed mycoplasm, and belongs to the technical field of traditional Chinese medicine and health food processing. According to the preparation method of the lucid ganoderma and coix seed mycoplasm, the lucid ganoderma and coix seed mycoplasm is obtained through liquid fermentation by taking coix seeds as a fermentation substrate and lucid ganoderma as a fermentation strain. The ganoderma lucidum and coix seed mycoplasm composition is obtained through a ganoderma lucidum and coix seed two-way fermentation technology, functional ingredients are increased, and the ganoderma lucidum and coix seed mycoplasm composition has the effects of enhancing immunity, protecting pancreas islet beta cells and improving glycolipid metabolism. The invention develops the application of the traditional Chinese medicine composition in the fields of food and health care products.

The application discloses an engineering biogas fermentation device and a method thereof. The fermentation device comprises a liquid fermentation material feeding mechanism and a solid fermentation material feeding mechanism. The liquid fermentation material feeding mechanism comprises a homogenate pool, which is connected with a feeding port of the fermentation device through a liquid inlet pipe. The solid fermentation material feeding mechanism comprises a feeding hopper and a conveying device. The conveying device comprises a motor and a conveying belt driven by the motor. The feeding hopper is arranged on the conveying belt. The solid fermentation material enters the feeding hopper and is fed to the fermentation device by the conveying device as needed. The liquid fermentation material feeding mechanism and the solid fermentation material feeding mechanism cooperate to form a mixed area for feeding in two directions at the bottom of the fermentation device. The application can reduce the methane emission of fermentation materials in the storage link in the station and the energy efficiency loss caused by invalid degradation of the fermentation materials. The application can provide high-quality fermentation raw materials for a biogas project and realize effective quality preservation and emission reduction.

This invention discloses a method, system, equipment, and medium for activating and promoting the release of humic acid from silkworm excrement. The method includes: mechanochemical synergistic crystal-breaking pretreatment, i.e., dry ball milling to destroy the physical encapsulation structure and induce a local alkaline microenvironment under chemical solvent-free conditions; stepwise enzymatic hydrolysis bridging activation, utilizing the acid buffering properties of silkworm excrement, sequentially adding uricase, cellulase / hemicellulase, and laccase or manganese peroxidase to form a natural pH fluctuation curve; directed microbial induction and release promotion, inoculating with Bacillus amyloliquefaciens and Bacillus lentigines for liquid fermentation to achieve metal ion chelation and surface charge regulation; and finally, low-temperature drying and molding to obtain the product. This invention also discloses the system, computer equipment, and storage medium for implementing this method. This invention does not require the addition of external acids, alkalis, or chemical chelating agents, shortens the total cycle to 2-4 days, and the obtained product has a high water-soluble humic acid content, which enhances the resistance to refixation after application to the soil.

The invention relates to the technical field of dairy product processing, in particular to a yeast protein dairy product and a preparation method thereof.In the yeast protein preparation process, the quality of the yeast protein dairy product can be remarkably improved through the synergistic effect of key processes such as bi-enzyme directional step-by-step hydrolysis, ultrafiltration desalination concentration, compound bacteria liquid fermentation and an emulsification compound system; the yeast dairy product prepared by the method can be further suitable for products such as high-protein beverages, cheese substitutes and stirred yoghurt, and has the advantages of nutrition retention and texture optimization.

The invention relates to the technical field of fermentation, in particular to family farm liquid fermentation feeding equipment which comprises a mixing device and at least one fermentation device, and the mixing device is communicated with each fermentation device. Each fermentation device comprises a fermentation tank, a second stirring mechanism and a second control assembly, the fermentation tanks are communicated with the mixing tank, the second stirring mechanisms are arranged on the fermentation tanks and used for stirring materials entering the fermentation tanks, and the second control assemblies are arranged on the fermentation tanks and used for controlling the materials in the fermentation tanks. In the fermentation process, multi-stage stirring is adopted, the Reynolds number Re of the materials is larger than or equal to 2000 in the fermentation process, the uniformity of the materials is improved, the number of viable bacteria of the materials is increased, and therefore the feeding error rate is reduced.

The invention discloses an automatic mixed fermentation system and process for preparing a traditional Chinese medicine multi-strain fermented beverage, and relates to the technical field of beverage production and fermentation. According to the automatic mixed fermentation system and process for preparing the traditional Chinese medicine multi-strain fermented beverage, through cooperation of the mixing frame mechanism, the driving mechanism, the cam frame mechanism, the gas pumping and discharging mechanism and the solution pumping and discharging mechanism, the driving mechanism can drive the mixing frame mechanism to rotate, liquid traditional Chinese medicine and various probiotics in the fermentation barrel are stirred and mixed, and the liquid traditional Chinese medicine and the various probiotics are mixed; the gas pumping and discharging mechanism and the solution pumping and discharging mechanism can also be driven to work, so that the gas pumping and discharging mechanism pumps external gas into the fermentation barrel, the dissolved oxygen amount in materials in the fermentation barrel is controlled, the dissolved oxygen requirement in the liquid state fermentation process is met, the solution pumping and discharging mechanism pumps external acid-base adjusting liquid into the fermentation barrel, and the fermentation efficiency is improved. The pH value of the material is maintained in a required range, the large fluctuation of the dissolved oxygen amount and the pH value caused by microbial metabolism in the fermentation process is prevented, and stable liquid fermentation is guaranteed.

The invention discloses a method for increasing the yield of monascus pigment through liquid fermentation, the monascus pigment is obtained through spore culture, seed culture, liquid fermentation and monascus pigment extraction by taking monascus as a production strain, and macroporous resin is added into a fermentation culture medium. According to the method, the yield of liquid fermentation of the monascus pigment can be greatly increased, the monascus pigment is concentrated in precipitates, namely resin and hyphae after solid-liquid separation is carried out on the fermentation product, a water phase of fermentation liquor does not contain pigment, separation and extraction of downstream fermentation products and treatment of fermentation waste liquor are facilitated, and the method has good market prospects and market value.

The application discloses an application of Armillaria mellea in degrading vomitoxin, and further discloses the Armillaria mellea Am-07-22 with a preservation number of CCTCC NO: M 20221044; the mycotoxin is vomitoxin or zearalenone. The strain Am-07-22 has the best degrading effect when the concentration of vomitoxin is 2 mu g / mL, and the degrading rate is 64.05%; the Armillaria mellea has the degrading effect on vomitoxin in two different fermentation raw materials, the degrading effect is good when adopting a solid-state fermentation mode for corn husks, and the degrading effect is good when adopting a liquid-state fermentation mode for corn yellow powder.

The present disclosure relates to a process for capturing CO2 using bio-digester slurry, along with a method to improve the quality of fermented organic manure (FOM) and liquid fermented organic liquor (LFOM). The present disclosure describes the use of lignin functionalizing microbes (LFM) and H2S oxidizing microbes (SOD) to enhance the quality of the FOM and LFOM and improve the CO2 capture ability of the slurry. The CO2 and / or H2S captured manure increases their nutrient value for use in agriculture. The CO2 absorption process not only aids in reducing the amount of greenhouse gases released into the environment, but also improves the quality of FOM and LFOM. The integration of these processes results in a sustainable and efficient approach for capturing CO2 while improving the quality of organic waste material for agricultural use.

The present application relates to a kind of for sensitive skin improves mood stress skin aging composition preparation and application, belong to cosmetic technical field.The raw material of the composition for sensitive skin improves mood stress skin aging provided in the present application includes mulberry root extract, figwort flower extract, with distance stone branch algae extract, palmitoyl tetrapeptide-7, wherein with distance stone branch algae extract is prepared by bidirectional liquid fermentation method, four ingredients scientific compound, form " source control cortisol-skin structure repair " complete path, through target point clear, ring interlocking synergies, it is realized to sensitive skin mood stress skin aging Systematic improvement, provides a kind of mild, efficient and mechanism clear solution for sensitive skin anti-aging.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The invention discloses a preparation process of a composition based on corn straw, corn stigma and grain juice, which comprises the following steps: solid state fermentation, liquid state fermentation, low temperature distillation, blending and filling to obtain the grain juice composition, the formaldehyde content of the grain juice composition is lower than 0.5 mg / L, and the grain juice composition is rich in corn peptide. According to the composition based on the corn straw, the corn stigma and the grain juice and the preparation process of the composition, the corn straw and the corn stigma are subjected to optimized solid-state and liquid-state fermentation respectively, and a specific low-temperature distillation technology is combined, so that efficient and mild extraction and conversion of nutritional ingredients and active substances in the raw materials are realized; according to the process, the content of bad byproducts such as formaldehyde in a final product is remarkably reduced, and meanwhile, rich natural active ingredients derived from raw materials are reserved to the greatest extent.

The present application belongs to the technical field of fermented feed, and particularly relates to a fermented feed prepared by using ganoderma lucidum, and a preparation method and application thereof. The present application takes by-products such as vinasse, cassava residue, potato residue, kelp residue, pepper residue and traditional Chinese medicine residue as substrates, through screening of fermentation strains, optimization of fermentation process and fermentation conditions, and by using liquid fermentation technology, a functional feed with low fermentation cost and rich in nutritional components is prepared by expanding the use of the above-mentioned unconventional feed resources, so that the purpose of replacing soybean meal as animal feed can be achieved, and food is saved. Meanwhile, the present application uses ganoderma lucidum as a fermentation strain, and the product after fermentation contains ganoderma polysaccharide, which can effectively protect the intestinal tract of animals and maintain the health of the intestinal tract of animals.

The invention discloses expansin recombinant aspergillus niger suitable for liquid fermentation as well as a construction method and application thereof, and belongs to the technical field of recombinant microorganisms. The technical problem of efficiently degrading cellulose is solved. The invention provides a recombinant aspergillus niger, and the recombinant aspergillus niger is obtained by taking aspergillus niger TH-2-D as a starting strain and replacing a saccharifying enzyme gene glaA as shown in SEQ ID NO.31 with a penicillium oxalicum expansin Swo gene as shown in SEQ ID NO.28. The invention also provides a preparation method of the recombinant aspergillus niger. The strain is subjected to liquid state fermentation, fermentation liquor is suitable for fiber puffing application, the cellulose degradation efficiency can be improved when the strain is used in cooperation with cellulase, linked starch can be effectively removed when the strain is used in cooperation with corn wet grinding enzyme, and the starch yield is increased.

The utility model relates to the technical field of liquid feeding, and provides an efficient intelligent liquid feeding device which comprises a storage device, a stirring cylinder, a liquid fermentation tank, a main feeding pipe, a main water inlet pipe, a centrifugal water pump, a feeding branch pipe, a water inlet branch pipe, a main discharging pipe, a first discharging pipe, a second discharging pipe, a water return tank, a main water return pipe and a water return branch pipe. The material storage device is connected with the stirring cylinders, the number of the stirring cylinders is two, the main feeding pipe is arranged at the bottom of the liquid fermentation tank, the main water inlet pipe is arranged at the top of the liquid fermentation tank, the centrifugal water pump is arranged on the main feeding pipe, the two feeding branch pipes are arranged on the main feeding pipe, and the centrifugal water pump is arranged on the main water inlet pipe. The stirring cylinder and the pipeline can be automatically cleaned after feeding, and the cleaned liquid is recycled to the water return tank, so that the cyclic utilization of water resources and the automatic operation and maintenance of the system are realized, the labor intensity of manual cleaning is reduced, and the water resource consumption and environmental pollution in the cleaning process are also reduced.

The invention discloses a chaetomium globosum solid-state fermentation extraction production system and method, and the production system comprises a metering and batching system which is used for weighing and metering raw materials required by preparation of a culture medium; the stirring and mixing tank is used for preparing a culture medium; the liquid fermentation tank is used for providing strain fermentation liquor; the solid-state fermentation tank is used for carrying out solid-state fermentation; the airing system is used for airing the solid-state fermentation product; the collecting system is used for collecting spores in the fermentation product; the metering and batching system conveys raw materials to the stirring and mixing tank through the conveying system, the solid-state fermentation tank is respectively connected with the stirring and mixing tank and the liquid-state fermentation tank through pipelines, the airing system is connected with the collecting system through a pipeline, and products obtained by the solid-state fermentation tank are conveyed to the airing system through the conveying system. The system and the method are suitable for chaetomium globosum solid state zymophyte and spore extraction, the chaetomium globosum solid state zymophyte and spore extraction is changed from a laboratory to a production line, and industrialization of chaetomium globosum fermentation and spore extraction is achieved.

The application discloses a kind of enzyme processing combination liquid fermentation production brewing type tobacco flavor agent's method, comprising the following steps: S1, cut tobacco is mixed with sorghum powder, after adding distilled water obtains tobacco-sorghum liquid medium;S2, yeast strain is inoculated to YPD culture medium and is cultivated, obtains yeast bacterial liquid;And / or lactobacillus strain is inoculated to MRS culture medium and is cultivated, obtains lactobacillus bacterial liquid;S3, configuration enzyme preparation solution, enzyme includes one or more of amylase, flavourzyme and saccharifying enzyme;S4, enzyme preparation solution is added to tobacco-sorghum liquid culture medium;S5, the yeast bacterial liquid and / or lactobacillus bacterial liquid of S2 is inoculated to the culture medium of S4 and is cultivated, obtains fermentation liquid flavor medium;S6, fermentation liquid flavor medium is freeze-dried, obtains tobacco flavor agent.The brewing type flavor agent of the application is prepared based on microbial fermentation technology, process is simple, condition is mild, cost is low, green environmental protection, improve the economic benefit of low quality cigarette.

The present application relates to the technical field of probiotic composition, and discloses a bacterial agent for regulating vaginal microbial flora and a preparation method thereof, which comprises the following steps: preparing Lactobacillus paracasei KFY202407 powder into a seed solution; performing segmented fermentation culture on the seed solution; obtaining bacterial slurry after centrifugal enrichment of the fermentation product; mixing the bacterial slurry with a composite freeze-drying protective agent and then performing freeze-drying to obtain freeze-dried bacterial powder; and performing gelatinization treatment on the freeze-dried bacterial powder to obtain the bacterial agent. The present application realizes rapid proliferation of bacterial cells and promotes accumulation of active metabolites by adopting a two-stage fermentation process of liquid-state fermentation followed by solid-state fermentation, thereby solving the technical problems of low active bacterial proliferation efficiency and insufficient metabolites in the prior art. Meanwhile, the present application adopts a freeze-drying process of two-stage pre-freezing and drying sublimation, so that ice crystals are removed in the form of sublimation instead of melting, thereby solving the technical problem of bacterial cell damage and uncontrollable inactivation due to ice crystals in the traditional freeze-drying technology.

The present application relates to a kind of tobacco flavor preparation method and its product and application, comprising the following steps: tobacco, sorghum and water are mixed to be sterilized, obtain tobacco-sorghum liquid medium, enzyme preparation and bacteria liquid are added to tobacco-sorghum liquid medium and are cultured fermentation, the bacteria liquid is yeast bacteria liquid and / or lactobacillus bacteria liquid, obtain the tobacco flavor.The present application uses sorghum and tobacco as common fermentation substrate, gives the double flavor of final flavoring liquor and tobacco flavor, using enzyme treatment combined with liquid fermentation method, solve the problem that the flavor brought by traditional stacking solid fermentation often needs to be passed through long time and flavor is insufficient etc., process is simple, condition is mild, green and environmental protection, cost is low, significantly improve the flavor quality of low quality cigar, improve the economic value of low quality cigar, with wide application prospect.

The invention belongs to the field of microorganisms, and particularly relates to a construction method and application of genetically engineered bacteria for super-high yield of monascus pigment. The invention discloses a construction method and application of a genetically engineered bacterium with superhigh yield of monascus pigment. The strain has the characteristic of superhigh yield of monascus pigment, and is obtained by taking red monascus as an original strain and screening by knocking out a gene MpSte11A. The invention discloses an application of the genetically engineered bacterium in microbial fermentation preparation of monascus pigment, and a liquid fermentation result shows that the highest intracellular total pigment content of MpSte11A on the 15th day is 112U / mL, which is 22 times of the yield (5.45 U / mL) of an original strain. The recombinant strain obviously promotes the production of monascus pigment under a liquid state fermentation condition, improves the fermentation efficiency, and has good commercial and scientific research values.