Organophosphorus pesticide degrading bacterium and method for producing microbial inoculum thereof

A technology for organophosphorus pesticides and degrading bacteria, which is applied in the biological field and can solve problems such as the influence of degradation efficiency, the Bacillus that degrades organophosphorus pesticides and the production method of its bacterial agent have not been found yet.

Inactive Publication Date: 2010-05-19
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For pesticide-degrading microorganisms isolated from soil, sludge and other sources, they may not be able to adapt to the harsh environment of plant foliage, and their degradation efficiency will often be sig...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Production of organophosphorus pesticide degrading bacterial agent

[0021] 1. Medium preparation:

[0022] 1) Strain preservation medium (solid, 1L): peptone 10g, yeast extract 5g, sodium chloride 5g, agar 20g, add water to 1L, pH 7.2;

[0023] 2) Strain activation medium (liquid, 1L): peptone 10g, yeast extract 5g, sodium chloride 5g, add water to 1L, pH 7.2;

[0024] 3) Seed medium (liquid, 10L): MgSO 4 1.0g, KH 2 PO 4 5.0g, NaCl 5.0g, K 2 HPO 4 15.0g, 100g sucrose, 50g peptone, add water to 10L, pH 7.2;

[0025] 4) Fermentation medium (liquid, 200L): MgSO 4 20g, KH 2 PO 4 100g, NaCl 100g, K 2 HPO 4 300g, molasses 12kg, (NH 4 ) 2 SO 4 0.8kg, add water to 200L, pH 7.2;

[0026] All the above media were sterilized at 121°C for 15 min.

[0027] 2. Strain activation: pick organophosphorus pesticide-degrading bacteria to the strain preservation medium, pick single colony by continuous streaking at 30°C for two times, then pick a single ...

Embodiment 2

[0031] Example 2: Degradation effect of organophosphorus pesticide degrading bacteria agent on dichlorvos pesticide residues on rape leaves

[0032] 1) Select two identical rape plots, each 30m 2 , evenly spray 1000 times diluted 80% dichlorvos EC pesticide;

[0033] 2) After 6 hours, evenly spray the pesticide-degrading bacteria agent (diluted 600 times with water), and use spraying water as a control;

[0034] 3) After 24 hours, the five-point sampling method was used to detect the residue of dichlorvos on rapeseed leaves, and it was found that the residue of dichlorvos in the treatment group was reduced by about 80% compared with the control group.

Embodiment 3

[0035] Embodiment 3: Degradation effect of organophosphorus pesticide degrading bacteria agent on rape leaf dimethoate

[0036] 1) Select two identical rape plots, each 30m 2 , evenly spray 1000 times diluted 50% Dimethoate EC pesticide;

[0037] 2) After 6 hours, evenly spray the pesticide-degrading bacteria agent (diluted 800 times with water), and use spraying water as a control;

[0038] 3) After 36 days, the five-point sampling method was used to detect dimethoate residues on rapeseed leaves, and it was found that the residues of dimethoate in the treatment group were reduced by about 65% compared with the control group.

[0039] The bacterial strain for degrading organophosphorus pesticides provided by the present invention is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation date is November 2, 2009, and the classification is named Bacillus subtilis (Bacillus subtilis). The deposit numb...

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PUM

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Abstract

The invention relates to bacillus capable of efficiently degrading an organophosphorus pesticide and separated from rape phyllospheres, and provides a method for preparing a microbial inoculum thereof, which belong to the field of biotechnology. The bacillus is identified as bacillus subtilis, which is preserved in the China General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, wherein the preservation No. is CGMCC No. 3374; the 16S rDNA gene sequence of the bacillus strain is submitted to a GenBank database; and the accession number is GU086422. The method for producing the microbial inoculum comprises the following 5 steps of: the preparation of a culture medium, the activation of a bacterium strain, the preparation of a liquid seed, the liquid state fermentation and the preparation of the microbial inoculum, wherein the fermentation culture medium takes molasses as a main raw material. Because the organophosphorus pesticide degrading microbial inoculum prepared by the technical scheme of the invention can efficiently degrade the organophosphorus pesticide residue on plant leaves in situ, the microbial inoculum can guarantee the safety of foods and protect the ecological environment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a bacillus isolated from the phyllosphere of rapeseed and capable of efficiently degrading organophosphorus pesticides, and a production method thereof. technical background [0002] Organophosphorus pesticides are still the most used category of pesticides because of their many varieties, high efficacy, and broad-spectrum prevention and control. They have played a pivotal role in agricultural production. More than half of the total production and usage. However, organophosphorus pesticides are highly toxic. After long-term and large-scale use, their residues on crops will cause food safety problems and affect the export of agricultural products in my country. Moreover, scattered pesticides will also pollute water and soil. [0003] The bioremediation method using microorganisms to degrade organophosphorus pesticide residues has the advantages of low cost, non-toxicity, and no seconda...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/125A62D101/04
Inventor 王晓辉姜丹李林杨建州刘冬芹白志辉
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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