Succinic acid purification process through anaerobic fermentation

A technology of anaerobic fermentation and succinic acid, which is applied in the field of bioengineering, can solve the problems of cumbersome steps, harsh operating conditions and high difficulty in the crystallization process, and achieve the effects of ensuring quality, reducing methanol residues, and reducing production costs

Inactive Publication Date: 2010-06-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operating conditions of the crystallization process are harsh, requiring high-temperature pyrolysis of sulfate, and the steps are cumbersome and difficult.
In a word, the current separation and extraction methods of succinic acid still need to be improved in terms of simplifying the process, improving product quality and reducing costs.

Method used

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  • Succinic acid purification process through anaerobic fermentation
  • Succinic acid purification process through anaerobic fermentation
  • Succinic acid purification process through anaerobic fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The method for fermentatively producing succinic acid described in the present invention can use any anaerobic fermentative microbial strain producing succinic acid in the prior art. The succinic acid-producing microbial strain used in this example is: Actinobacillus succinogenes NJ113 (Actinobacillus succinogenes NJ113). This bacterium has applied for a patent and obtained authorization, and the patent authorization announcement number is CN100537744C.

[0037] The fermentation medium of the present invention can use any fermentation medium for producing succinic acid by anaerobic fermentation in the prior art. The medium used in this example is: every liter of volume medium contains 50-100 g of glucose (sterilized separately), 15 g of yeast powder, 15 mL of corn steep liquor, 1.36 g of anhydrous sodium acetate, 1 g of NaCl, K 2 HPO 4 3g, MgCl 20.2g, CaCl 2 0.2g.

[0038] The method for preparing succinic acid by fermenting microbial strains of the present invent...

Embodiment 2

[0050] The present embodiment adopts the same fermentation conditions and methods as in Example 1, and its whole process is as follows: image 3 Shown:

[0051] NaOH was used to adjust the fermentation pH.

[0052] In the anaerobic reactor, succinic acid is produced by anaerobic fermentation of microorganisms. During the fermentation process, the pH of the fermentation system is adjusted with NaOH and maintained at 7.0. After the fermentation, the fermented liquid is filtered to filter out insoluble matter, and then ultra-filtered to remove proteins, polysaccharides and other biological macromolecules to avoid the production of pigments in the subsequent concentration and crystallization process; at the same time, a decolorization unit is added before the multi-effect evaporation, To ensure the quality of succinic acid crystallization products; in the crystallizer, the pH is maintained at 1.8, and at this low pH, CO 2 , and add ammonia water, disodium succinate reacts with a...

Embodiment 3

[0061] The present embodiment adopts the same fermentation conditions and methods as in Example 1, and its whole process is as follows: Figure 4 Shown:

[0062] Using Mg(OH) 2 Adjust the fermentation pH.

[0063] Using carbohydrates as a carbon source, succinic acid is produced by anaerobic fermentation of microorganisms in an anaerobic reactor. During the fermentation process, Mg(OH) 2 Adjust the pH of the fermentation system to maintain around 6.8. After the fermentation, the fermented liquid is filtered to filter out insoluble matter, and then ultra-filtered to remove proteins, polysaccharides and other biological macromolecules to avoid the production of pigments in the subsequent concentration and crystallization process; at the same time, a decolorization unit is added before the multi-effect evaporation, To ensure the quality of succinic acid crystallization products; the pH in the crystallizer is maintained at 1.6, at this low pH, add HCl, and then add ammonia wate...

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Abstract

The invention belongs to the technical field of biological engineering and discloses a novel succinic acid purification process through anaerobic fermentation. By adopting the separation process based on solid-liquid separation, ultrafiltration, decoloration, acidification and crystallization and by introducing ammonium sulfate thermal cracking technology, the invention realizes cyclic utilization of the acid and the alkali, forms a closed clean production flow and effectively ensures the quality of the succinic acid used for food and medicine. The invention not only reduces the fermentation cost and improves the economic benefits but also reduces environment pollution, improves the ecological environment and promotes the sustainable development of the national economy. Therefore, the invention has wide social benefits.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to a novel closed process for purifying succinic acid by anaerobic fermentation. Background technique [0002] Succinic acid, also known as succinic acid, is a common natural organic acid widely found in the human body, animals, plants and microorganisms. At present, most of succinic acid is still synthesized by chemical method, and its raw material depends on fossil resources. The industrial potential of succinic acid fermentation was recognized as early as 1980. With the depletion of fossil resources and the technological progress of biological preparation of succinic acid, the large-scale production of succinic acid by biotransformation has attracted more and more countries. attention. [0003] Succinic acid is the most important C4 platform compound in biorefinery product engineering. As many important intermediate products and professional chemicals, the traditional prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C07C55/10C07C51/42C07C51/43C12R1/01
Inventor 姜岷李建陈可泉吴昊黄秀梅方晓江韦萍欧阳平凯
Owner NANJING UNIV OF TECH
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