Method of manufacturing cordyceps sinensis (Berk.) sacc polysaccharide by liquid state fermentation of rice bran and bran complete feed

A technology of Cordyceps sinensis polysaccharide and liquid fermentation, applied in the direction of fermentation, etc., can solve the problems of high price of Cordyceps sinensis, reduce the price of Cordyceps sinensis products, etc., and achieve the effects of advanced methods, protection of public health, and reduction of production costs.

Inactive Publication Date: 2013-12-04
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of Cordyceps sinensis is currently high, and it is difficult for ordinary people to consume in large quantities. In order to further utilize the value of Cordyceps sinensis and expand its application advantages, it is necessary to study new production methods of Cordyceps sinensis polysaccharides, and convert some low-end raw materials such as rice bran into high-value Cordyceps polysaccharides reduce the price of Cordyceps products so that ordinary people can consume them and promote public health

Method used

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  • Method of manufacturing cordyceps sinensis (Berk.) sacc polysaccharide by liquid state fermentation of rice bran and bran complete feed
  • Method of manufacturing cordyceps sinensis (Berk.) sacc polysaccharide by liquid state fermentation of rice bran and bran complete feed

Examples

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Effect test

Embodiment 1

[0022] Cordyceps sinensis strain adopts Cordyceps sinensis CCTCC M2013285. The amount of rice bran used is 3.0g / 100mL medium, the amount of bran used is 3.0g / 100mL medium, and KH 2 PO 4 0.09g / 100mL, MgSO 4 0.04g / 100mL, pH is natural, add water to the required volume, sterilize at 121°C and cook for 30 minutes, use it as a medium for fermentation, the sample volume of shake flask is 40%, the inoculum volume is 10%, the culture temperature is 28°C, the rotation speed 180r / min, culture time 5d. The mycelium containing a small amount of rice bran bran obtained from liquid culture is centrifuged and washed with water, the mycelium is extracted with hot water at 70°C for 3 hours, and the clear liquid is obtained by filtration. The centrifuged liquid of fermented products or mycelia extracted by broken hot water, after deproteinization, alcohol precipitation, centrifugal separation, and vacuum drying, exopolysaccharide and mycelial polysaccharide were obtained respectively, and th...

Embodiment 2

[0024] Cordyceps sinensis strain adopts Cordyceps sinensis CCTCC M2013285. The amount of rice bran used is 1.0g / 100mL medium, the amount of bran used is 1.0g / 100mL medium, and KH 2 PO 4 0.09g / 100mL, MgSO 4 0.04g / 100mL, pH is natural, add water to the required volume, sterilize at 121°C and cook for 30 minutes, use it as a medium for fermentation, the sample volume of shake flask is 40%, the inoculum volume is 10%, the culture temperature is 28°C, the rotation speed 180r / min, culture time 5d. The mycelium containing a small amount of rice bran bran obtained from liquid culture is centrifuged and washed with water, the mycelium is extracted with hot water at 70°C for 3 hours, and the clear liquid is obtained by filtration. The centrifuged liquid of fermented products or mycelia extracted by broken hot water, after deproteinization, alcohol precipitation, centrifugal separation, and vacuum drying, exopolysaccharide and mycelial polysaccharide were obtained respectively, and th...

Embodiment 3

[0026] Cordyceps sinensis strain adopts Cordyceps sinensis CCTCC M2013285. The amount of rice bran used is 3.0g / 100mL medium, the amount of bran used is 3.0g / 100mL medium, and KH 2 PO 4 0.18g / 100mL, MgSO 4 0.08g / 100mL, natural pH, add water to the required volume, sterilize at 121°C and cook for 30 minutes, use it as a medium for fermentation, the sample volume of the shake flask is 40%, the inoculum volume is 10%, the culture temperature is 23°C, the rotation speed 150r / min, culture time 7d. The mycelium containing a small amount of rice bran bran obtained from liquid culture is centrifuged and washed with water, the mycelium is extracted with hot water at 70°C for 3 hours, and the clear liquid is obtained by filtration. The centrifuged liquid of fermented products or mycelia extracted by broken hot water, after deproteinization, alcohol precipitation, centrifugal separation, and vacuum drying, exopolysaccharide and mycelial polysaccharide were obtained respectively, and t...

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Abstract

A manufacturing method for cordyceps sinensis (Berk.) sacc polysaccharide relates to the technical field of food microorganism application, and comprises the following steps: weighing raw materials, wherein rice bran is 1-3 g per 100 ml, bran is 1-3 g per 100 ml, potassium dihydrogen phosphate is 0.09-0.18 g per 100 ml, and magnesium sulphate is 0.04-0.08 g per 100 ml; material filling with natural pH after adding required water, and sterilizing and cooking at the temperature of 121 DEG C for 30 min; inoculating cordyceps sinensis (Berk.) sacc CCTCCM2013285 liquid seed after cooling, wherein the inoculation amount is 8-10%, the fermentation temperature is 23-28 DEG C, and the fermentation time is 4-7 d; respectively obtaining exopolysaccharides and mycelia polysaccharide after conducting deproteinization, alcohol precipitation, centrifugal separation, and vacuum drying on centrifugal separation liquid extracted through breaking cell wall hot water of fermentation material centrifugal separation liquid and hypha. The method better realizes the purpose that rice bran and bran are efficiently and high-valuedly transformed to cordyceps sinensis (Berk.) sacc polysaccharide after being subjected to liquid state fermentation.

Description

technical field [0001] The invention relates to the technical field of food microorganism application, in particular to a method for high-yielding the polysaccharide of Cordyceps sinensis by liquid fermentation of rice bran bran whole material culture medium with Cordyceps sinensis strain. Background technique [0002] Since Chihara et al. (references: Chihara G, MaedaY, Hamuro J, et al. Inhibition of mouse sarcoma180by polysaccharides from Lentinus edodes (Berk.) sing [J]. Nature, 1969, 222 (5194): 687-688.) In 1969, he published a paper on "Nature" and revealed that lentinan has anti-tumor activity. More than 200 medicinal fungi with anti-tumor activity have been found, some of which are edible fungi, such as Grifola frondosa and Hericium erinaceus , Ganoderma lucidum, Cordyceps sinensis, etc. The mycelium, fruiting bodies, sclerotia or spores of these edible fungi can produce various active ingredients such as amino acids, proteins, vitamins, polysaccharides, glycosides,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04
Inventor 刘伟民陈静沈国栋周文彪赵杰文马海乐张笑飞曹勋金雷
Owner JIANGSU UNIV
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