The invention relates to a sandwich candy floss with donkey hide glue and a preparation method thereof. The candy floss comprises the following ingredients according to parts by weight: 0.3-0.5 parts of donkey hide glue, 2-3 parts of modified starch, 35-40 parts of white granulated sugar, 2-4 parts of gelatin, 0.3-0.5 parts of pectin, 55-60 parts of water, 0.1-0.2 parts of vitamin C and 0.1-0.2 parts of vitamin E. Based on the traditional candy floss formula, the sandwich candy floss is added with the donkey hide glue and the pectin, the water using amount is increased, the using amounts of the modified starch and the gelatin are reduced, the added pectin can retain water completely, so that the cost is lowered, simultaneously, the candy floss body is good in elasticity, and is softer, can be dissolved upon entry into the mouth, and has the same health protection function as that of the donkey hide glue, which can nourish blood and beautify a human body. The preparation method of the sandwich candy floss has the advantages of low cost and high production efficiency.