144results about How to "Optimizing Fermentation Conditions" patented technology

The invention relates to a biocontrol strain Y-1 for preventing and controlling fungal diseases of grapes and application of the biocontrol strain Y-1 in prevention and control of grape diseases and belongs to the technical field of biological prevention and control of plant diseases. The strain is Meyerozyma guilliermondii Y-1 with a preservation number of CCTCC (China Center For Type Culture Collection) NO:M2015814. The Meyerozyma guilliermondii Y-1 is used for preparing a biocontrol preparation for preventing and controlling the grape diseases, and the biocontrol preparation has an obviousprevention and control effect on multiple pathogenic fungi. The Meyerozyma guilliermondii Y-1, provided by the invention, as an active ingredient of the biocontrol preparation, has the characteristicsof stable prevention and control effect, long persistence and wide disease control spectrum as well as has the properties of environment friendliness, safety and low possibility of generating pesticide resistance; the strain has high propagation speed, is cultured under simple culture conditions and is easy for storage and industrial production; defense related enzyme activity in plant bodies canbe improved, and a good development and application prospect is realized.

The invention relates to a phellinus igniarius bacterial strain liquid fermenting culture medium and a method for fermenting and producing phellinus linteus polysaccharides. The culture medium has a prescription shown as follows: 20 to 30g/L of glucose, 2 to 6g/L of yeast powder, 1 to 3g/L of KH2PO4, 0.3 to 0.7g/L of MgSO4.7H2O, 110mg/L of VB, and 1L of water; a pH is under a natural condition; a fermenting condition is that a temperature is at 28 to 32 DEG C, a rocking speed is at 140 to 180rpm, inoculation amount is 20 to 30%, cultivation is carried out for 9 to 11 days; the method comprises the following steps of: centrifuging, collecting fermenting liquid, washing via distilled water, precipitating to obtain a mycelium, then extracting polysaccharide inside or outside cells. With the adoption of the phellinus igniarius bacterial strain liquid fermenting culture medium and the method for fermenting and producing the phellinus linteus polysaccharides provided by the invention, yield of the mycelium of phellinus igniarius and content of the polysaccharide inside or outside the cells can be obviously increased; moreover, yields of the phellinus linteus polysaccharides is 6.34% higher than that of polysaccharide extracted by a common hot water extracting method. An animal test proves that extracted phellinus linteus polysaccharides have obvious biological activity such as tumour resistance, oxidization resistance, and improvement of immunity.

The invention relates to a Bacillus siamensis YJ15 and an application thereof in biological control of blueberry gray mold. Bacillus siamensis strain YJ15 of that invention belong to the genus Bacillus sp., Deposited in the Common Microorganism Center of China Microorganism Culture Collection Management Committee (CGMCC), strain collection No. CGMCC No. 15952. Bacillus siamensis YJ15 is inoculatedin LB liquid culture medium to obtain fermentation seed liquid, and then the fermentation seed liquid is inoculated in fermentation culture liquid to obtain fermentation broth or supernatant of fermentation broth to obtain biological microbial agent. The biological bacterial agent prepared by the invention can control the blueberry gray mold and has good control effect equivalent to carbendazim.

The invention discloses a plant and fruit fermentation stock solution. A preparation method of the plant and fruit fermentation stock solution comprises the following steps: cleaning A-type fruits including 3-5 parts of green plums, 2-4 parts of waxberries, 2-3 parts of strawberries, 3-6 parts of raspberries and 1-2 parts of avocados, wiping and carrying out fermentation; cleaning B-type fruits including 5-6 parts of blueberries, 3-4 parts of mulberries, 5-7 parts of cherries, 3-5 parts of plums, 2-3 parts of prunes and 1-2 parts of wax apples, wiping, feeding 2 parts by weight of maltose, and carrying out fermentation; preparing C-type fruits including 4-5 parts of avocado, 2-3 parts of mangosteens and 1-2 parts of kiwi fruits, and carrying out fermentation; cleaning plants including ginseng which is artificially planted for 4-5 years, Chinese wolfberries, burdock root and pueraria (peeled), and carrying out fermentation; and carrying out fermentation termination treating, namely, uniformly mixing 5 parts of fermentation filtrate of the A-type fruits, 2 parts of fermentation filtrate of the B-type fruits, 1 part of fermentation filtrate of the C-type fruits and 3 parts of fermentation filtrate of the plants to obtain a mixture, immediately putting the mixture into a closed fermentation vat, and continuously fermenting for one month to obtain the finished product of the plant and fruit fermentation stock solution.

A preparing method of a long-acting organic fertilizer is provided and belongs to the technical field of agrochemistry. According to the method, a humus forming amount is increased through raw material formula regulation, microbial inoculum improvement to inhibit acidification and ammonification, an aerobic compost fermentation process is accelerated through equipment of a compost process, and ammonia nitrogen in fermentation tail gas is recovered to prepare guano phosphorite. The method has characteristics of high innovation, simple steps, a low equipment investment, and convenient preparation, and the produced organic fertilizer is stable and long-acting in fertility, high in humus contents and good in environment benefit and has good popularization value.

The invention discloses a preparation method and applications of freeze-dried powder of acetic acid bacteria. The preparation method comprises following steps of: 1) inoculating the acetic acid bacteria into a liquid culture medium and culturing at 28-30 DEG C for 10-30 h to obtain a primary strain; 2) inoculating the primary strain into a fermentation culture medium, culturing at 28-33 DEG C for 10-30 h, and adding an aqueous sodium hydroxide solution having a concentration of 5-25 wt% during the fermentation process to obtain a fermentation liquid mixture; 3) filtering the fermentation liquid mixture or centrifuging and separating to obtain bacterial sludge; 4) uniformly mixing the bacterial sludge and a bacterial sludge protective agent to obtain a bacterial suspension; and 5) freeze-drying the bacterial suspension to obtain the freeze-dried powder of the acetic acid bacteria. After the freeze-dried powder of the acetic acid bacteria is mixed with various additives, alcoholism-relieving tablets and alcoholism-relieving beverages, which have good alcoholism-relieving effects, can be obtained through different processes. The preparation method is simple and the freeze-dried powder of the acetic acid bacteria is high in bacterium content and long in storage time.

The invention relates to construction of saccharomyces cerevisiae engineering bacteria generating beta-phenethyl alcohol, a strain and application thereof, and belongs to the technical field of bioengineering and production of food spices. The prepared beta-phenethyl alcohol spice is a natural spice and can be used for preparing a plurality of food essences, and therefore, the beta-phenethyl alcohol can be widely applied to industries such as food, medicine and daily use chemicals. Engineering bacterial for coupling overexpression of an aminotransferase gene (ARO8) and decarboxylase gene (ARO10) in the anabolic way of beta-phenethyl are designed and constructed with saccharomycescerevisiae S288 as an original strain; gene copy and expression of the two key enzymes in the main synthetic way are enhanced; the bred engineering bacteria (transformants) are passaged a plurality of times and stable in inheritance; the metabolic flux and the yield of beta-phenethyl are obviously improved. With L-phenylalanine as a major raw material, the engineering bacteria are used for fermenting and converting the raw material into the beta-phenethyl alcohol spice which is single in configuration and chirality and high in fragrance quality, and therefore, a new idea and technical support are provided for preparation of the natural beta-phenethyl alcohol spice.

The invention relates to a spiral type fermentation turner which is applied to an aerobic fermentation process of various organic materials including fertilizers, feeds and foods, and realizes forcedventilation oxygen-increasing operation of the materials simultaneously when turning and stirring the fermentation materials. The spiral type fermentation turner comprises a walk device arranged on astand of the spiral type turner, turning and stirring devices and a forced ventilation oxygen-increasing device and is characterized in that one or more rows of turning and stirring devices are installed below the stand, tow rows of branch ventilating pipes which extend into the bottom of the fermentation materials are vertically and symmetrically installed at the axial line position in the middleof two adjacent turning and stirring devices in the same row, and gas supply equipment is connected with the branch ventilating pipes through a main ventilating pipe. When turning and stirring the fermentation materials, the spiral turner can ensure true realization of forced ventilation and oxygen increasing to the materials, increase the smoothness and the safety of walking of the turner, enable the fermentation period to be shortened by over 70 percent and the turnover rate of a fermentation workshop to be enhanced by over 50 percent, also lower the energy consumption in the fermentation and production process by over 50 percent and dramatically enhance the economic benefit of an enterprise.

The invention discloses an antibiotic peptide and its preparation and application. According to sequence of natural antibiotic peptide, gene of antibiotic peptide is designed and synthesized in DNA synthesizer by choosing genetic code preferred by yeast. The invention selects dispense of culture medium and optimizes condition of fermentation. The antibiotic peptide can kill many pathogenic microorganisms such as Gram-positive bacteria, Gram-negative bacterium and so on.

The invention discloses a preparation method of a malt wort fermented beverage. The preparation method comprises the following steps: (1) preparing malt meal; (2) preparing malt wort; (3) domesticating lactobacilli, namely mixing the malt wort with pure milk in different volume ratios respectively to prepare a domestication culture medium, and inoculating mixed bacteria into the domestication culture medium to obtain mixed lactobacilli liquid containing domesticated lactobacilli; (4) performing primary fermentation on the lactobacilli; (5) performing secondary fermentation on the lactobacilli; and (6) blending, namely adding white granulated sugar and a stabilizer into the malt wort to obtain the malt wort fermented beverage. According to the preparation method, the technical difficulty that the lactobacilli unfavorably grow in the malt wort is overcome, the mixed lactobacilli can be directly fermented and the practicability of lactic acid fermentation is improved through a lactobacilli domestication process. The malt wort fermented beverage prepared by adopting the method is sour, sweet and palatable, soft and coordinated in mouth feel, rich and lasting in fragrance and full in aftertaste, retains the fragrance and nutritional ingredients of malt and is added with nutritional substances generated by the lactobacilli.

The invention discloses bacillus subtilis, a fermentation method, powder and application. The bacillus subtilis is bacillus subtilis Gv-1, the depository authority is the China Center for Type Culture Collection, the depository address is Wuhan University, Wuhan of China, the depository number is CCTCC NO: M2015766, and the depository date is December 21, 2015. According to the invention, Kosteletzkya virginica earthnut endophyt is separated from a Kosteletzkya virginica earthnut for the first time, and purification, 16S rDNA sequencing and morphological and physiological characteristic analysis are performed, so that isolated and cultured stra Gv-1 is identified as bacillus subtilis. According to the invention, bacillus subtilis powder is prepared through bacillus subtilis fermentation liquor and is added into pig feed, and results show that the pig feed with the bacillus subtilis powder added can sufficiently realize the effect of the bacillus subtilis Gv-1, promote the production performance of piglets and digestive absorption for protein, Ca and P by the piglets, improve the intestinal microorganism flora of the piglets, and ensure that the intestinal microorganism flora is in an optimum equilibrium state.

The invention relates to high-yield 3-Methionol saccharomyces cerevisiae engineering bacteria and a preparation method and application thereof, and belongs to the technical field of biological preparation of food flavors. According to the high-yield 3-Methionol saccharomyces cerevisiae engineering bacteria and the preparation method and application thereof, the engineering bacteria is designed and constructed through the coupling excessive expression of an aminotransferase gene (ARO9) and a decarboxylase gene (ARO10) and the knock-out of a methylthio lyase gene (CY3) in the anabolic pathway of Methionol by taking Saccharomyces cerevisiae S288c as an original strain; the expression of two key enzyme genes in a main synthetic pathway is enhanced, and the expression of the CY3 gene in a side reaction pathway is reduced or eliminated; the bred engineering bacteria (converter) is subjected to multiple-time passage and stable in inheritance, so that the metabolic network and metabolic pathway of the biological synthesis of the Methionol are optimized, and the metabolic flux and yield of the Methionol are obviously increased; a Methionol flavor prepared by fermenting and converting the engineering bacteria is single in structure and chirality and high in fragrance quality.

The invention provides a method and a special-purposed strain used for producing alpha-ketoglutaric acid. Lichtheimia corymbifera D1 provided by the invention has a collection number of CGMCC NO.4974. The lichtheimia corymbifera D1 provided by the invention has good strain stability, good alpha-ketoglutaric acid production capacity, relatively low fermentation cost (substrates have wide source and low cost), and good industrialized application prospect. The invention also optimizes the fermentation conditions of lichtheimia corymbifera D1. The method provided by the invention is used for producing alpha-ketoglutaric acid, and has good scientific research value and industrial application prospect.

The invention discloses a modifying method for sandy soil. The modifying method for the sandy soil comprises the following steps: step (1), turning the sandy soil; step (2) laying a straw layer in a soil pit; and uniformly spreading a microbial agent on the straw layer, wherein the microbial agent comprises one or more of bacillus subtilis, lactobacillus and saccharomycetes; step (3) laying a fermenting layer on the microbial agent, wherein the fermenting layer comprises bean dregs, vinasse and water which are mixed; step (4) laying a tree leaf layer on the fermenting layer; and step (5) fermenting tree leaves and green grass in a fermenting tank for 20 days to obtain a fermented product, wherein fermenting culture is bacillus subtilis; cutting straws into straw particles, blending the straw particles in the turned soil, blending the fermented product in the straw particles, and covering the tree leaf layer with the soil blended with the straw particles. By the modifying method, a soilenvironment is improved, a soil structure is modified, and finally, a soil desertification condition is improved.

The invention provides a method for preparing a composite amino acid liquid fertilizer by biologically degrading feather. The method comprises the following steps: 1) inoculating bacillus licheniformis with a preservation number of CGMCC NO.17458 into a seed culture medium, and carrying out culturing to obtain a seed solution; 2) adding feather into a fermentation culture medium to prepare a feather fermentation culture medium; and 3) inoculating the seed solution into the feather fermentation culture medium, and carrying out fermenting to obtain the composite amino acid liquid fertilizer. Thecompound amino acid liquid fertilizer can effectively promote growth of crops. The method for preparing the liquid fertilizer disclosed by the invention has the advantages of simple production condition, low investment, high efficiency and remarkable product growth promoting effect, and is easy to popularize and apply. According to the invention, waste feather resources can be effectively recycled, and meanwhile, ecological circulating cultivation of poultry can be promoted.

The invention discloses a method for improving clear degree of Luzhou-flavor liquor by pit mud maintenance, and belongs to the technical field of wine making. According to the method, high-quality aged pit mud functional micropopulation is used as a seed, and performed with culture intensification through a specific culture medium; then the micropopulation and wine brewing yeast are commonly rejuvenated and cultivated. The matured compound bacteria compound and liquid esterifying enzyme are added in the brewing pit mud and the yeast of the Luzhou-flavor liquor together and feed into the pit together. Due to the synergistic effect of the pit mud functional micropopulation and the yeast, the substrate inhibition is weakened, and the esterification of the external esterifying enzyme reduces the substrate inhibition, so that the caproic acid fermentation activity of the pit mud functional micropopulation is significantly improved; concentrations of caproic acid and ethyl caproate in a raw liquor are improved by 2-3 times; meanwhile, the clear level of the liquor body is greatly improved. The method provides a basis for the controllable high-quality brewing of the Luzhou-flavor liquor.

The invention discloses a method for improving the yield of L-arginine synthesized by corynebacterium crenatum, specifically discloses a method for improving the yield of L-arginine through integratedmutation modification of bi-functional uridyl transfer/removal enzyme GlnD, modification of pII signal transduction protein GlnK and uridine acylation of GlnK, and belongs to the technical field of bioengineering. The method successfully achieves the integrative mutation modification of the bifunctional uridyl transfer/removal enzyme GlnD, the pII signal transduction protein GlnK is overexpressedon this base, and the uridine acylation of GlnK is enhanced. A 5-L fermentation tank is used for batch fermentation, the fermentation conditions are optimized, finally the final recombinant corynebacterium crenatum Cc-HDAA/pXMJ19-glnK is fermented for 96 h, the yield of L-arginine of the recombinant bacteria reaches 52.2 g/L, the production intensity reaches 0.544 g/L h, and the high yield of L-arginine is achieved.

The invention relates to optimization of a methyl nutritional type bacillus spore-production fermentation medium. The fermentation medium consists of the following components: 0.5-0.6% of corn meal, 0.08-0.2% of corn starch, 0.9-1.2% of bran, 0.18-0.54% of glucose, 0.05-0.15% of soybean cake powder, 0.16-0.2% of soybean peptone, 0.005-0.006% of KH2PO4, 0.006-0.008% of (NH4)2SO4, 0.004-0.005% of MnSO4, 0.0005-0.002% of CaCO3, 0.005-0.006% of MgSO4.7H2O, and the balance of water. The culture medium provided by the invention not only can meet the requirement of a shake flask fermentation experiment but also can successfully amplify the result of shake flask fermentation to a fermentation tank. Compared with the culture medium before optimization, the spore conversion rate and the spore yield of the formula of the culture medium are improved by 1-10 times, and the formula of the culture medium adopts low-price agriculture byproducts, so that the production cost is greatly reduced, and a foundation is laid for large-scale industrial production of wettable powder.

The invention discloses a preparation method of thermostable chitosanase. The method comprises the following steps: cloning a chitosanase gene having a high catalytic activity and a lasting normal-temperature enzyme activity, constructing a chitosanase engineering bacterium having a high catalytic activity and a lasting normal-temperature enzyme activity, and preparing the chitosanase BmCsn havinga high catalytic activity and a lasting normal-temperature enzyme activity. The chitosanase has an obvious stability, and the enzyme activity of the chitosanase undergoing heat insulation at 28 DEG Cfor 7 d is only 5% less than the initial activity. The stability of the chitosanase is higher than the stability of reported general products. After the fermentation conditions of the chitosanase areoptimized, so the protein content reaches up to 4.5 g/L. After the chitosanase undergoes high-density fermentation, the enzyme activity reaches up to 834 U/mL fermentation solution.

The invention discloses a method for modifying desertified soil. The method comprises the steps of step (1) desertified soil is turned over, the turning depth is 30-40 cm, the turned soil is placed aside, and a pit is formed; step (2) a straw layer is laid in the pit; the straw layer is sprayed with a microbial agent uniformly, and the microbial agent comprises one or more of bacillus subtilis, lactic acid bacteria and saccharomycetes; step (3) a fermentation layer is laid on the microbial agent and comprises bean dregs, distilled grain and water, and the mass ratio of the fermentation layer and the microbial agent is (20-25):1; step (4) a leaf layer is laid on the fermentation layer; step (5) the straw is cut into straw granules, the straw granules are stirred into the turned soil, and the leaf layer is covered with the soil stirred with the straw granules. According to the method for modifying the desertified soil, the soil environment is improved, the soil structure is modified, andfinally the soil desertification condition is improved.

A livestock and poultry manure fermentation treatment method is disclosed. Livestock and poultry manure fermentation treatment equipment composed of a fermentation roller, a supporting device, a drive unit, a feeding and discharging device, a heating and stirring device and a ventilation unit is adopted. Through a feed screw conveyer inside the feeding and discharging device, fresh livestock and poultry manure which needs to be treated is conveyed into the fermentation roller; through rotating of the fermentation roller, ripened materials containing high-temperature fermentation strains are mixed in proportion; the product then undergoes continuous stirring, heating and dehydration, etc., and rationed oxygen supply at fixed time cooperates; and finally, completely fermented ripened materials are sent out, and potassium, phosphor or beneficial trace elements are added so as to form an organic compound fertilizer. By the method, manure can reach the most efficient fermentation temperature and water content. In addition, with rationed oxygen supply at fixed time, fermentation speed and fermentation effect are greatly improved. By adding potassium, phosphor or beneficial trace elements into the completely ripened livestock and poultry manure, utilization value of livestock and poultry manure is raised.

The invention discloses a method for preparing a fermentative mirror carp surimi sausage through a pediococcus pentosaceus fermentation agent. The method is characterized in that mirror carps are adopted as raw materials and are prepared into surimi through the steps of pretreatment, chopping, mixing, strain cultivation, fermentation agent inoculation, optimizing of fermentation conditions, canning and storage. The method aims at solving the processing problem that freshwater surimi is serious in fishy smell and difficult to store, the method is combined with the microbial fermentation technology commonly used by modern meat paste, meat paste products of the highly developed mirror carp industry in the northeast are systematically studied, and the processing technique for the fermentative mirror carp surimi sausage is formed; and the quality characteristic of the mirror carps is improved through fermentation, the fishy smell is weakened, and the shelf life of surimi products is prolonged by maintaining better freshness.

The invention discloses bacillus subtilis for efficiently producing beta glucanase as well as an application thereof. The bacillus subtilis is named as Bacillus subtilis pj101. The strain has been already stored in China General Microbiological Culture Collection Center on January, 28, 2012, wherein the preserving number is CGMCC No.6897. The invention further provides an application of the bacillus subtilis strain for efficiently producing beta glucanase in fermenting production of beta glucanase. The bacillus subtilis strain CMGCC 6897 is good in genetic stability, and beta glucanase produced is high and stable in activity. In addition, the fermenting condition of the strain is further optimized to obtain a better enzyme producing effect, so that bacillus subtilis can be applied to industrial fermenting production of beta glucanase.