22results about How to "Optimizing Fermentation Conditions" patented technology

The invention belongs to the technical field of biochemical engineering, and discloses application and a method of tea polyphenol in improvement of schizochytrium limacinum DHA (docosahexaenoic acid) grease yield and grease storage, and tea polyphenol is added in the process of producing DHA by fermentation of schizochytrium limacinum. By comparing the difference of schizochytrium limacinum in tea polyphenol fermentation and traditional fermentation, it is clear that the antioxidant tea polyphenol regulates cell oxidative stress in schizochytrium limacinum, removes ROS and inhibits grease oxidation, so that grease rancidity is delayed, and grease synthesis and DHA production are increased. According to the invention, schizochytrium limacinum HX-308 is used as a receptor strain, and an antioxidant tea polyphenol is added for regulation, so that fermentation conditions under which the content of grease and DHA is obviously increased and the grease storage effect is better are obtained.

The invention relates to alpha-amylase, an alpha-amylase gene, an engineering bacterium for high yield of alpha-amylase and application of the engineering bacterium, and belongs to the technical field of biology and the technical field of enzyme engineering. According to the invention, an alpha-amylase gene is identified from a strain A8-1 with high starch hydrolysis activity, and the alpha-amylase coded by the alpha-amylase gene has the advantages of strong pH stability, wide temperature resistance range and strong resistance to organic solvents. Furthermore, by heterologous expression of the amylase gene in different hosts, a recombinant strain BAX-5 / PTamyy (A8-1) with the highest enzymatic activity is obtained, and the enzymatic activity of the recombinant strain is 156.1 U / mL. Finally, through signal peptide and promoter engineering, a recombinant strain BAX-5 / PTmy (A8-1) SP002 with the highest enzyme activity is obtained, and the enzyme activity reaches 205 U / mL.

The invention belongs to the technical field of biology, and discloses a biological utilization method of brewer's grains, which comprises the following steps: fermenting a solid culture medium containing the brewer's grains by adopting a strain to obtain a biological feed; the strain contains aspergillus oryzae. Aspergillus oryzae is adopted as one of zymophytes and can significantly reduce the content of pentosan, and as a preferable scheme, the aspergillus oryzae, lactobacillus plantarum and bacillus subtilis are combined to further reduce the content of pentosan, improve the content of crude protein and improve the application value of the feed. Meanwhile, the invention further provides the biological feed prepared on the basis of the method and application of the biological feed.

The utility model discloses a fermentation tank for optimizing fermentation conditions, which comprises a tank body, a rotating shaft arranged in the tank body, and an optimization assembly arranged on the rotating shaft, the optimization assembly comprises a plurality of blades arranged at the bottom end of the rotating shaft, and a plurality of blades arranged above the blades, the stirring rods are arranged in the tank body and connected with the rotating shaft, the liquid spraying pipe penetrates through the rotating shaft, the liquid spraying pipe is obliquely arranged, and a plurality of disturbance rods are connected to the outer side of the liquid spraying pipe and used for optimizing the mixing rate of fermentation liquor and thalli in the tank body. According to the utility model, the blades, the stirring rods and the liquid spraying pipe are arranged on the rotating shaft, so that thalli gathered at the bottom of the tank body are sequentially subjected to turning raising, stirring mixing, upward throwing-out and backflow stirring, so that fermentation conditions are optimized during thalli fermentation, the mixing speed of the thalli and fermentation liquor is accelerated, and the fermentation efficiency is improved. And the uniform mixing state can be quickly achieved.

The invention relates to a production device for shrimp culture fermented feed. Impurities in agricultural waste are removed by fine screening and the ratio of water to carbon source is adjusted to ensure that the materials entering the fermentation tank are uniform and suitable for microbial decomposition. A power device drives a stirring shaft and related components to achieve efficient mixing, crushing and primary fermentation strain inoculation. A high-pressure oxygen supply system and intelligent temperature control technology are combined to maintain optimal fermentation conditions and effectively improve the decomposition efficiency of organic matter. In addition, waste gas generated by primary fermentation is recovered and burned to convert into heat energy for heating a fermentation tank body to achieve energy recycling. In the process of material transfer, an adjusting component is used for fine cutting and secondary crushing. At the same time, a specific functional microbial preparation is added in the lower inner tank body to enrich the nutrient components of the feed, thereby achieving efficient conversion of agricultural waste resources and improving the economic benefits of the fermentation process.

The invention relates to the technical field of data processing, in particular to a yeast frozen dough fermentation process control method based on a fermentation kinetic model. The method comprises the following steps: acquiring frozen dough data and yeast activity data; constructing a yeast dynamic basic model according to the yeast activity data to obtain the yeast dynamic basic model; performing dough component feature extraction according to the frozen dough data to obtain frozen dough component feature data; performing yeast fermentation dynamic evolution according to the frozen dough component characteristic data and the yeast dynamic basic model to obtain frozen dough fermentation process data; and performing fermentation process evaluation according to the frozen dough fermentation process data to obtain fermentation process evaluation data so as to perform yeast frozen dough fermentation process control auxiliary operation. The control method of the fermentation process can be effectively simulated, so that the fermentation uniformity and stability of the frozen dough are improved, and the quality of baked products is further improved.

The utility model discloses a saccharification pile oxygen introducing device in the technical field of white spirit brewing, which comprises a vertical pipe and a coil pipe, the vertical pipe is vertically arranged, the top end and the bottom end of the vertical pipe are sealed, a plurality of exhaust holes penetrating through the inner wall and the outer wall are formed in the peripheral side of the vertical pipe, and a connecting hole penetrating through the inner wall and the outer wall is formed in the bottom end of the vertical pipe. A plurality of vent holes penetrating through the inner wall and the outer wall are formed in the circumferential side of the coil pipe, an air inlet in one end of the air conveying pipe is connected with the air supply device, and the other end of the air conveying pipe is connected with the pipe opening in the outermost ring of the coil pipe. The saccharification pile oxygen introducing device solves the technical problems that fermentation is insufficient and sauce fragrance is not prominent enough due to the fact that the oxygen content of the fermentation pile core and the pile bottom of an existing saccharification pile is low, and is simple in structure and capable of improving the oxygen content of the pile core and the pile bottom of the saccharification pile.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis GS33 and application thereof. The fermentation supernatant of the bacillus laicisii GS33 has broad-spectrum antibacterial activity on common food-borne pathogenic bacteria and plant fungi, can generate a large number of bacteriostatic active substances after fermentation optimization, can be further developed as a probiotic agent, a growth promoting agent or an antibacterial agent, can promote animal growth or prevent diseases after being added into feed, and has a good application prospect. Therefore, the use of antibiotics can be reduced to a certain extent, and the application prospect is wide. According to the invention, the fermentation conditions of the bacillus velezensis GS33 for producing the bacteriostatic active substances are also optimized, and the bacteriostatic activity of the fermentation supernatant is improved by 30.14% through optimization of the fermentation conditions, so that the yield of the bacteriostatic active substances is effectively improved. A powerful tool is provided for solving the problems of drug resistance, drug residues, environmental pollution and the like caused by the application of antibiotics in livestock production.

The invention provides a fingered citron tea beverage with high oxidation resistance and a preparation method thereof, and solves the problems of poor oxidation resistance and heavy bitter taste in the prior art. The preparation method of the fingered citron tea beverage with high oxidation resistance comprises the following steps: S1, preparing sweet water; s2, selecting dry pieces of Jinhua fingered citron, namely boy chokeberry, and brewing tea soup; s3, filtering tea soup; and S4, co-fermenting by using kombucha, and centrifuging to obtain supernate. The fingered citron tea beverage prepared from Jinhua fingered citron boy has high oxidation resistance, and the fingered citron tea beverage can effectively neutralize free radicals in a human body and reduce oxidative stress due to the high oxidation resistance, so that the risks of cardiovascular diseases and the like are reduced. Besides, the kombucha liquid selected in the invention is used as a leavening agent, so that the total antioxidant capacity of the boy chokeberry fingered citron tea soup is improved from 2.25 U / mL before fermentation to 3.95 U / mL after fermentation, and the increase amplitude reaches 75.56%. The combination of the selected kombucha and Jinhua fingered citron boy has a remarkable synergistic effect, and the oxidation resistance of the fingered citron tea beverage can be greatly improved.

The invention belongs to the technical field of microbial fermentation, and discloses a fermentation medium for producing Ectoine engineering strains, a fermentation method and application. The fermentation medium for producing the Ectoin engineering strain comprises the following components: 5g / L-35g / L of glucose, 5g / L-30g / L of peptone, 5g / L-30g / L of yeast extract powder, 20mM-250mM of sodium aspartate, 2g / L-12g / L of ammonium sulfate, 5g / L-10 g / L of monopotassium phosphate, 10g / L-18g / L of dipotassium phosphate, 0.5 g / L-2g / L of magnesium sulfate, 0.5 g / L-2g / L of citric acid monohydrate and 0.05 g / L-0. 5g / L of compound vitamin B. According to the fermentation method disclosed by the invention, a double-flow feeding mode of simultaneously feeding the carbon source and the nitrogen source is adopted for feeding, so that the yield of the Ectoin at a fermentation tank level is remarkably improved, and a technical support is provided for industrial-scale production of the Ectoin.

The invention relates to a fermentation method of erythritol, and belongs to the technical field of erythritol fermentation. The method comprises a fermentation strain shake flask culture stage, a primary seed tank expansion culture stage and a fermentation stage. The erythritol fermentation production method adopts a batch sugar supplementation mode to be put into a fermentation tank. The batch sugar supplementation fermentation is adopted, which is conducive to extending the fermentation cycle but not too long, and the time is controlled within the range of 70 to 100 hours, thereby improving the fermentation conversion rate, effectively improving the erythritol yield, and being suitable for large-scale industrial production.

The invention discloses recombinant escherichia coli for producing NMN as well as a preparation method and application of the recombinant escherichia coli, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, nicotinamide phosphoribosyltransferase NAMPT as shown in SEQ ID NO.1, PRPP synthase BaPRS as shown in SEQ ID NO.2 and transporter BMpnuC as shown in SEQ ID NO.3 are expressed in escherichia coli, and a pncC gene, a ushA gene, an nadR gene and a purR gene are knocked out, so that the accumulation amount of NMN in escherichia coli cells is increased. In addition, by optimizing fermentation conditions, NMN can be efficiently accumulated outside cells, 20 g / L or above of NMN can be obtained under a 5L fermentation system, and the method has wide application prospects in the fields of medicines, cosmetics, feed, textiles and the like.

The invention relates to the technical field of organic fertilizer production, in particular to an organic fertilizer production process and equipment based on three-source fermentation, and the process comprises the following steps: crushing, screening and mixing fermentation raw materials to obtain a fermentation base material; stirring and mixing the fermentation base material, water and the fermentation culture medium additive to obtain a fermentation mixed matrix; stirring the fermentation mixed matrix for fermentation, obtaining the mixed gas production speed of mixed gas generated in the stirring process in the detection period to adjust the fermentation temperature in the fermentation tank, and detecting the microbial concentration to obtain the actual microbial concentration; the fermentation stirring speed is adjusted to meet the fermentation requirement under the condition that the microbial concentration cannot meet the fermentation requirement; whether fermentation is stopped or not is judged by detecting oxygen consumption and fermentation temperature changes, and the fermented mixed substrate is guided into an organic fertilizer separation device to obtain the organic fertilizer. By accurately controlling the particle size of the fermentation base material and adjusting the fermentation temperature, the microbial activity is improved, and the quality of the organic fertilizer is improved.

The invention relates to the technical field of food processing, in particular to a constant-temperature quantitative inoculation device for dregs vinegar fermentation, which comprises a tank body, a spraying assembly is arranged in the tank body, an inoculation assembly is arranged at the top, and a temperature control pipeline is arranged on the outer side wall; the inoculation assembly comprises a sterile inoculation box, a connecting pipeline and a metering pump, and the spraying assembly is driven by an electric sliding rail; a temperature sensor is arranged in the tank body, a pressure sensor, a sterile air inlet and a one-way exhaust valve are arranged on the top of the tank body, and a side wall control panel is integrated with a wireless transmission module and linked with all parts. The device can accurately control the temperature and quantitatively inoculate, construct a sterile fermentation environment, realize uniform distribution of spraying and real-time monitoring and regulation of parameters, solve the problems of large quality fluctuation and high risk of infectious microbe pollution of a traditional device, improve the fermentation efficiency and the product percent of pass, and is suitable for large-scale production of dregs vinegar.

The invention relates to the technical field of biological fermentation, and discloses a fruit and vegetable and wild tea grass enzyme efficient fermentation method based on active bacteria, and the method comprises the following steps: S1, screening and gene optimization of diversified active bacteria: screening strains from a natural environment and a commercial strain library, the glycometabolism capability and the environmental stress resistance of the strain are enhanced through a genetic engineering technology; s2, performing pretreatment and enzymolysis on the fruit and vegetable raw materials: performing mechanical crushing and multi-enzyme synergistic treatment on the fruit and vegetable raw materials to improve the enzymolysis efficiency; and S3, staged dynamic fermentation: performing saccharification and fermentation in stages under different temperature and pH conditions. Through diversified flora screening and gene optimization, the enzyme activity is remarkably improved; by combining staged dynamic fermentation and an intelligent control technology, the fermentation efficiency and the process stability are improved; ultrahigh-pressure and low-temperature plasma non-heat treatment is adopted, so that the stability and shelf life of the product are enhanced, and the requirements of the market on high-quality enzyme products are met.

The invention discloses a black tea fermentation liquid with antioxidant and brightening effects, a preparation method and application thereof, and belongs to the technical field of cosmetics. The black tea fermentation liquid with antioxidant and brightening effects of the invention is obtained by fermenting black tea powder with composite bacteria, wherein the composite bacteria are animal Bifidobacterium lactis subspecies and Lactobacillus plantarum; the end mark of the fermentation is: the pH of the black tea fermentation liquid drops to 3.5-4.5. The invention adopts specific composite bacteria to ferment black tea powder, and optimizes the conditions during the fermentation process, so that the prepared black tea fermentation liquid has excellent antioxidant, brightening, firming and soothing effects.

The invention relates to an antibiotic-free biological fermentation compound feed for poultry and a preparation method thereof, and belongs to the technical field of feeds, the feed takes a fermentation basic raw material as a carrier, plant extracts and chemical addition components are added, and the synergistic effects of resisting bacteria, diminishing inflammation, regulating intestinal flora, promoting nutrient absorption and improving the immunity of poultry are achieved in a biological fermentation synergistic manner; and antibiotic residues are avoided, so that the safety of poultry products is guaranteed.

The invention discloses black tea fermentation equipment, and belongs to the field of tea leaf fermentation equipment.The fermentation chamber is internally provided with multiple layers of fermentation bins for containing black tea, the fermentation chamber is made of heat preservation materials, and each layer of fermentation bin is internally provided with a temperature sensor and a humidity sensor; the temperature control system comprises a heater and a cooling system, and the heater and the cooling system can automatically and adaptively adjust the temperature of the fermentation chamber according to sensor data; the humidifier sprays water mist into the fermentation chamber, the dehumidifier comprises a suction pump and a filter ball connected with the suction pump, the filter ball comprises a hollow spherical shell, the spherical shell is filled with a drying agent, the suction pump is installed at the bottom end in the fermentation chamber, and when the suction pump is started, after water vapor in air in the fermentation chamber is captured by the filter ball, the air in the fermentation chamber is discharged out of the fermentation chamber. And directly discharging into a corresponding fermentation bin. According to the invention, black tea fermentation can be rapidly and efficiently carried out, the fermentation conditions are accurately and rapidly controlled, and the energy-saving property is good.

The invention provides Xiangyuwang essence and a preparation method thereof. Comprising the following components in parts by mass: 85 to 97 parts of propylene glycol, 1.5 to 3 parts of butyric acid, 0.1 to 0.3 part of octanoic acid, 0.4 to 0.8 part of decanoic acid, 0.1 to 0.4 part of ethyl lactate, 2 to 4 parts of vanillin, 0.2 to 0.4 part of ethyl maltol, 1 to 2 parts of delta-decalactone, 1 to 2 parts of delta-dodecalactone, 0.4 to 0.7 part of gamma-dodecalactone, 1 to 3 parts of butanedione, 0.3 to 0.5 part of ethyl butyrate, 0.6 to 0.9 part of butanedione, 0.3 to 0.5 part of 2-heptanone and 4 to 6 parts of a milk-flavored fermentation product. The Xiangyuwang essence prepared by the preparation method disclosed by the invention is pure, rich and mellow in fragrance, long in aftertaste and rich in fermentation taste, and can be widely applied to various beverages, dairy products, ice cream, baked foods, candies and the like.

The invention relates to the technical field of rice vinegar preparation, in particular to olive rice vinegar and a preparation method thereof. The method specifically comprises the following steps: (1) soaking glutinous rice, cooking, liquefying and saccharifying to obtain saccharified glutinous rice; (2) mixing the saccharified glutinous rice and olive pomace to obtain a fermentation system, and mixing the fermentation system with a fermentation inoculant for fermentation to obtain vinegar mash; (3) filtering the vinegar mash with water to obtain a primary filtrate, mixing and soaking the vinegar mash and the olive juice, and filtering to obtain a leachate; and mixing the primary filtrate and the leachate to obtain the olive rice vinegar. The invention aims at developing and optimizing a new technology of the olive rice vinegar, the prepared rice vinegar is good in taste and excellent in quality, and reference is provided for subsequent research on the quality and technology of the olive rice vinegar.

The invention relates to the field of feed, in particular to a preparation method of traditional Chinese medicine residue feed and application of the traditional Chinese medicine residue feed in mutton sheep breeding. According to the preparation method of the traditional Chinese medicine residue feed provided by the invention, the content of harmful substances can be effectively reduced through specific fermentation process treatment, so that the nutritional ingredients of the feed are converted and improved; meanwhile, the obtained fermented traditional Chinese medicine residues are rich in nutritional ingredients such as protein, cellulose and mineral substances, are easy to digest and absorb and can be used as a high-quality feed raw material. The invention further provides a mutton sheep feed containing the traditional Chinese medicine residues. The feed has the advantages of being safe to use, promoting the growth and development of mutton sheep and improving the mutton quality. Therefore, the traditional Chinese medicine residues can be used as a novel feed resource through microbial fermentation, and nutrient substances are provided for growth and development of ruminants.

This invention belongs to the field of bioengineering technology, specifically relating to a preparation process for nano-sized water-soluble squalene. The process includes the following steps: S1, corn flour is treated and then mixed with pure water, followed by enzymatic hydrolysis using a compound enzyme to obtain a corn saccharification liquid for later use; S2, *Saccharomyces cerevisiae* GS-A3 is inoculated into a culture medium containing the corn saccharification liquid for fermentation; S3, after centrifugation of the fermentation broth, crude squalene is obtained by supercritical CO2 extraction; S4, after molecular distillation, the product is nano-sized. This invention uses corn as raw material, utilizing abundant starch resources, converting it into glucose through enzymatic hydrolysis, and producing squalene in *Saccharomyces cerevisiae* via the MVA pathway. This invention prepares high-yield, high-purity squalene, and through a special nano-saccharification process, improves the stability of squalene, extends its shelf life during application, and significantly improves its bioavailability. Furthermore, the technology used in this invention meets the requirements of green environmental protection.