Preparation method and applications of freeze-dried powder of acetic acid bacteria

A technology for freeze-dried bacteria powder and acetic acid bacteria strain, which is applied in the field of preparation of acetic acid bacteria freeze-dried bacteria powder, can solve the problems of too much lightening of liver burden, slow reaction speed, unsatisfactory effect and the like, achieves protection of the liver, reduces The speed of metabolism, the effect of good hangover effect

Inactive Publication Date: 2014-05-28
天津百利食品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although vinegar can alleviate the anesthesia effect of excessive alcohol, because the above-mentioned esterification reaction is carried out in the human body, it will be interfered by many factors, and the reaction speed is very slow, hydrochloric acid is needed as a catalyst,

Method used

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  • Preparation method and applications of freeze-dried powder of acetic acid bacteria
  • Preparation method and applications of freeze-dried powder of acetic acid bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of freeze-dried powder of Acetobacter pasteurianus and production of hangover tablet

[0031] 1) Inoculate the Acetobacter pasteurii strain into the liquid medium first, and cultivate it at 28°C for 10 hours to obtain the first-grade strain, and the Acetobacter pasteurii strain accounts for 0.1% of the mass of the liquid medium; The composition and mass percentage of the liquid medium are glucose 2%, yeast extract 2%, MgSO 4 .7H 2 O0.05%, KH 2 PO 4 0.05%, absolute ethanol 4%, and the rest is water; during the preparation of the liquid medium, first mix the components except absolute ethanol evenly, sterilize at 121°C for 15 minutes, then add absolute ethanol, and the liquid culture base pH = 6.0;

[0032] 2) Insert the primary strain into the fermentation medium and culture it at 28°C for 20 hours. At the same time, add 5% sodium hydroxide aqueous solution during the fermentation process to maintain the pH of the fermentation medium = 5.0-6.5 to obtain a...

Embodiment 2

[0039] Preparation of freeze-dried powder of Acetobacter oxydans and production of hangover tablet

[0040] 1) Inoculate the strain of Acetobacter oxydans into the liquid medium first, and culture it at 30°C for 15 hours to obtain the first-grade strain, and the strain of Acetobacter oxidans accounts for 0.05% of the mass of the liquid medium; The composition and mass percentage of the liquid medium are glucose 2.5%, yeast extract 2.5%, MgSO 4 .7H 2 O0.01%, KH 2 PO 4 0.1%, absolute ethanol 7%, and the rest is water; in the process of liquid medium preparation, firstly mix the components except absolute ethanol, sterilize at 121°C for 15 minutes, then add absolute ethanol, the liquid culture base pH = 6.0;

[0041] 2) Insert the primary strain into the fermentation medium and culture it at 30°C for 22 hours. At the same time, add 10% sodium hydroxide aqueous solution during the fermentation process to maintain the pH of the fermentation medium = 5.0-6.5 to obtain a fermenta...

Embodiment 3

[0048] Preparation of freeze-dried powder of Acetobacter hansenii and production of hangover drink

[0049] 1) Put the Acetobacter hansenii strains into the liquid medium first, and cultivate them at 30°C for 30 hours to obtain the first-grade strains, and the Acetobacter hansenii strains account for 0.01% of the liquid medium mass %; The composition and mass percent of the liquid medium are glucose 1%, yeast extract 1%, MgSO 4 .7H 2 O0.01%, KH 2 PO 4 0.3%, absolute ethanol 10%, and the rest is water; in the process of liquid medium preparation, first mix the components except absolute ethanol evenly, sterilize at 121°C for 15 minutes, then add absolute ethanol, the liquid culture base pH = 6.0;

[0050] 2) Insert the primary strain into the fermentation medium and culture it at 33°C for 30 hours. At the same time, add 25% sodium hydroxide aqueous solution during the fermentation process to maintain the pH of the fermentation medium at 5.0-6.5 to obtain a fermentation mixt...

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Abstract

The invention discloses a preparation method and applications of freeze-dried powder of acetic acid bacteria. The preparation method comprises following steps of: 1) inoculating the acetic acid bacteria into a liquid culture medium and culturing at 28-30 DEG C for 10-30 h to obtain a primary strain; 2) inoculating the primary strain into a fermentation culture medium, culturing at 28-33 DEG C for 10-30 h, and adding an aqueous sodium hydroxide solution having a concentration of 5-25 wt% during the fermentation process to obtain a fermentation liquid mixture; 3) filtering the fermentation liquid mixture or centrifuging and separating to obtain bacterial sludge; 4) uniformly mixing the bacterial sludge and a bacterial sludge protective agent to obtain a bacterial suspension; and 5) freeze-drying the bacterial suspension to obtain the freeze-dried powder of the acetic acid bacteria. After the freeze-dried powder of the acetic acid bacteria is mixed with various additives, alcoholism-relieving tablets and alcoholism-relieving beverages, which have good alcoholism-relieving effects, can be obtained through different processes. The preparation method is simple and the freeze-dried powder of the acetic acid bacteria is high in bacterium content and long in storage time.

Description

technical field [0001] The invention relates to the field of biological health food, in particular to a preparation method and application of acetic acid bacteria freeze-dried bacteria powder. Background technique [0002] With the continuous progress and development of society, alcohol is playing an increasingly important role in people's social life, and alcohol culture is also popular all over the world, but excessive drinking can cause serious damage to the liver, because excessive drinking means that excessive alcohol exceeds Alcohol will cause direct or indirect damage to liver cells if the metabolism and detoxification capabilities of the human liver are impaired. Each of the three metabolic steps of alcohol in liver cells has potential toxicity to cells, such as direct damage to liver cells, stimulation of fat synthesis, hypoxia, and production of acetaldehyde, which induces the activities of various enzymes and disrupts liver metabolism, causing a series of clinical...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K36/05A61K35/74A61P25/32C12R1/02
Inventor 赵金梁赵发
Owner 天津百利食品股份有限公司
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