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Method and application for preparing composite amino acid liquid fertilizer by biologically degrading feather

A compound amino acid and biodegradation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of easy destruction of amino acid components, complicated processing procedures, low amino acid yield, etc., and achieve easy absorption and utilization of crops , low fermentation temperature requirements, large consumption of feathers

Active Publication Date: 2019-08-30
江苏丘陵地区南京农业科学研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current production of amino acid fertilizers that are mostly used in acid hydrolysis or alkali hydrolysis feathers, the amino acid components are easily destroyed, there are many chloride ions in the product and it is difficult to remove, there is a risk of secondary pollution in the production process, and the existing fermented feather amino acid raw materials are processed The present invention provides a method for preparing compound amino acid liquid fertilizer by using biodegradable feathers and the application of the compound amino acid liquid fertilizer due to the complex process, many medium components and low amino acid yield

Method used

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  • Method and application for preparing composite amino acid liquid fertilizer by biologically degrading feather
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  • Method and application for preparing composite amino acid liquid fertilizer by biologically degrading feather

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Effect test

Embodiment 1

[0031] 1. Degrading bacteria

[0032] The Bacillus licheniformis used in the present invention is a biologically safe strain of Bacillus licheniformis with high protease activity obtained by the applicant's screening and isolation. The applicant named it ZSZ6 and preserved it in China on March 28, 2019. General Microbiology Center (CGMCC), Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, Institute of Microbiology, Chinese Academy of Sciences, Zip Code: 100101, and the deposit number is: CGMCCNO.17458.

[0033] 2. Activation of strains

[0034] Streak the Bacillus licheniformis ZSZ6 frozen at -80°C on the activation medium and culture at 37°C for 12-24h;

[0035] Activation medium formula: yeast powder 5g, NaCl 10g, tryptone 10g, agar powder 15g, add water to 1L, adjust pH to about 7.2, sterilize and pour plate for later use.

[0036] 3. Preparation of seed solution

[0037] A single colony of Bacillus licheniformis ZSZ6 was picked and inoculated...

Embodiment 2

[0055] Embodiment 2 prepares compound amino acid liquid fertilizer

[0056] The preparation steps are as follows;

[0057] 1. Pick a single colony of Bacillus licheniformis ZSZ6 and inoculate it into the seed medium, culture it on a shaker at 37°C and 180rpm for 12-18h, and prepare the seed solution;

[0058] The formula of the seed medium is: 5g of yeast powder, 10g of NaCl, 10g of tryptone, add water to make up to 1L, adjust the pH to about 7.2, and sterilize for later use.

[0059] 2. Feathers are added to the fermentation medium in an amount of 5% by mass, and sterilized at 121°C for 20 minutes to make a feather fermentation medium;

[0060] The formula of the fermentation medium is (1L): 1g of potassium dihydrogen phosphate, 1g of dipotassium hydrogen phosphate, 1g of sodium chloride, the balance is water, and the pH value is 7.0;

[0061] 3. Inoculate the seed liquid prepared in step 1 into the hair fermentation medium according to the volume ratio of 2%, with a liquid...

Embodiment 3

[0065] Application of embodiment 3 compound amino acid liquid fertilizer

[0066] 1. Growth-promoting test

[0067] The experiments were all carried out in the cultivation experiment area of ​​Nanjing Institute of Agricultural Sciences in the hilly area of ​​Jiangsu.

[0068] (1) Growth-promoting test of Chinese cabbage.

[0069] The compound amino acid organic liquid fertilizer prepared by the present invention is used in a potted test of Chinese cabbage. Establish 3 treatments altogether, be respectively the compound amino acid organic liquid fertilizer (I) that embodiment 2 obtains, commercially available amino acid fertilizer (Yishibang, Nongfei (2014) Zhunzi No. 3632, CN6121403-isa02) (II) and clear water (CK ). Fertilize after the seeds germinate and grow 2 to 3 true leaves, the amount of water per pot is 45ml, and the nitrogen content of the fertilizer applied in each treatment is 0.16g L -1 , CK poured the same amount of water. A total of 4 times of pouring, each ...

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Abstract

The invention provides a method for preparing a composite amino acid liquid fertilizer by biologically degrading feather. The method comprises the following steps: 1) inoculating bacillus licheniformis with a preservation number of CGMCC NO.17458 into a seed culture medium, and carrying out culturing to obtain a seed solution; 2) adding feather into a fermentation culture medium to prepare a feather fermentation culture medium; and 3) inoculating the seed solution into the feather fermentation culture medium, and carrying out fermenting to obtain the composite amino acid liquid fertilizer. Thecompound amino acid liquid fertilizer can effectively promote growth of crops. The method for preparing the liquid fertilizer disclosed by the invention has the advantages of simple production condition, low investment, high efficiency and remarkable product growth promoting effect, and is easy to popularize and apply. According to the invention, waste feather resources can be effectively recycled, and meanwhile, ecological circulating cultivation of poultry can be promoted.

Description

technical field [0001] The invention relates to the field of resource utilization of waste, in particular to a method for producing compound amino acid liquid fertilizer by using bacillus licheniformis to biodegrade feathers and the application of the compound amino acid liquid fertilizer. Background technique [0002] Amino acids, as the smallest molecules that make up proteins, exist in fertilizers, which are easy to be absorbed by crops; they also have the functions of improving the disease resistance of fertilized objects and improving the quality of fertilized crops. The free amino acids in fertilizers can be directly absorbed and utilized by plants, improving the utilization rate of fertilizers and the conversion rate of crop nutrients, which is of great significance to the growth of plants. At present, methods such as fermentation of soybean meal and fish meal, acid hydrolysis of sick and dead livestock and poultry, and enzymatic hydrolysis of feathers and blood are m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F1/00C05F17/00C12N1/20C12R1/10
CPCC05F1/005C12R2001/10C12N1/205C05F17/20Y02A40/20Y02W30/40
Inventor 周影王琳魏启舜郭成宝文蔚明
Owner 江苏丘陵地区南京农业科学研究所
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