Bacillus subtilis, fermentation method, powder and application

A technology of Bacillus subtilis and a fermentation method, applied in the field of Bacillus subtilis, can solve the problems of long fermentation period, high cost, low spore formation rate, etc., and achieve the effects of low cost, easy access to sources, and improved stress resistance

Active Publication Date: 2016-05-25
河南广安生物科技股份有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the industrial production of Bacillus subtilis, there are still problems such as long fermentation period, low sporulation rate and high cost.

Method used

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  • Bacillus subtilis, fermentation method, powder and application
  • Bacillus subtilis, fermentation method, powder and application
  • Bacillus subtilis, fermentation method, powder and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Isolation, purification, identification and preservation of seashore mallow root tuber endophytes

[0035] 1. Isolation and purification of seaside mallow tuber endophytic bacteria

[0036] The seaside mallow material is taken from the seaside mallow root tubers grown in the first year in mainland China introduced from the United States. The coastal mallow planting test area is located at 34°83′N, 113°50′E, in the tidal flat of the Yellow River in Huiji District, Zhengzhou City, Henan Province. The seaside mallow planted with seeds enters the dormant period in winter after a one-year growth cycle, and the underground part of the tubers are taken out as a whole as experimental materials.

[0037]The seashore mallow tubers freshly dug out in the field were cleaned, ventilated and dried, and the plant epidermis was removed with a scalpel under aseptic conditions. Soak in 70% ethanol for 1min, wash with sterile water three times, soak in sodium hypochlorite for ...

Embodiment 2

[0086] Embodiment 2 Bacillus subtilis Gv-1 influences on plant growth

[0087] Wheat was used as experimental material to test the effect of Bacillus subtilis Gv-1 on plant growth. The cultivation container of wheat is a black plastic nutrition bowl with a diameter of 8 cm and a depth of 1.2 cm at the bottom. The nutrition bowl is filled with vermiculite with a depth of 7 cm and sterilized at 121° C. for 1 hour.

[0088] Disinfection treatment of wheat seeds: soak in 75% ethanol for 1 min, then soak twice in 5% sodium hypochlorite solution, 10 min each time. 5 grains of wheat seeds are sown in each nutrient bowl, and the nutrient bowl is placed in a square dish and water is added from the bottom to make the vermiculite fully absorb water. After the seeds germinated, the nutrient bowl was irrigated with 1 / 2 Hoagland nutrient solution, placed in a light intensity of 5000 lux, and cultivated at 22°C, with 16 hours of light and 8 hours of darkness, alternate cultivation. After a...

Embodiment 3

[0094] The fermentation culture of embodiment 3 Bacillus subtilis Gv-1

[0095] 1. Optimization of fermentation and culture conditions of Bacillus subtilis

[0096] 1.1 Condition optimization of fermentation medium

[0097] Wash, dry and cut seaside mallow roots first, add water to boil 3 times, each time for 30 minutes, filter, combine three boiling filtrates, add water 3 times the weight of seaside mallow root each time, and obtain sea ice mallow root extract .

[0098] Mix sea-ice mallow tuber root extract and ordinary broth medium according to the volume ratio of 100:0, 70:30, 60:40, 40:60 and 80:20 respectively to obtain a mixed culture solution, and then add the mixed culture medium Liquid total weight 0.02% (NH 4 ) 2 SO 4 and 0.04% NaOH, adjust the pH to 9.0, and sterilize under high pressure at 121° C. for 30 minutes to obtain 5 kinds of fermentation media.

[0099] Inoculate the preserved Bacillus subtilis Gv-1 into a 50mL Erlenmeyer flask containing 30mL of LB ...

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Abstract

The invention discloses bacillus subtilis, a fermentation method, powder and application. The bacillus subtilis is bacillus subtilis Gv-1, the depository authority is the China Center for Type Culture Collection, the depository address is Wuhan University, Wuhan of China, the depository number is CCTCC NO: M2015766, and the depository date is December 21, 2015. According to the invention, Kosteletzkya virginica earthnut endophyt is separated from a Kosteletzkya virginica earthnut for the first time, and purification, 16S rDNA sequencing and morphological and physiological characteristic analysis are performed, so that isolated and cultured stra Gv-1 is identified as bacillus subtilis. According to the invention, bacillus subtilis powder is prepared through bacillus subtilis fermentation liquor and is added into pig feed, and results show that the pig feed with the bacillus subtilis powder added can sufficiently realize the effect of the bacillus subtilis Gv-1, promote the production performance of piglets and digestive absorption for protein, Ca and P by the piglets, improve the intestinal microorganism flora of the piglets, and ensure that the intestinal microorganism flora is in an optimum equilibrium state.

Description

technical field [0001] The invention relates to a strain of Bacillus subtilis, and also relates to a fermentation method of the Bacillus subtilis, a powder containing the Bacillus subtilis and its application, belonging to the technical field of bioengineering. Background technique [0002] Seashore mallow (Kosteletzkyavginica) is a stress-resistant plant that tolerates salt and can use saline-alkali land and dry soil. It originally grew on the east and south coasts of the United States, and was introduced to China by scientists after 1992. Seaside mallow seeds contain higher unsaturated fatty acids and higher protein, and its roots contain polysaccharides, saponins and other ingredients and anti-cancer active ingredients, which can improve the production performance and immunity of animals, and have the potential to be used as feed, medicine, etc. potential. [0003] Bacillus subtilis is one of the two types of bacillus that are allowed to be added as ingredients in my cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/30A23K10/18A01N63/00A01P21/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 高天增郭予琦张天勇高鹏飞郭小云符锋管军军毛晶晶李梦君
Owner 河南广安生物科技股份有限公司
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