44results about How to "High number of viable bacteria" patented technology

The invention relates to the technical field of biological products, and discloses a fermentation method for lactobacillus with high cell density. The fermentation method comprises the steps of constructing a fibrous bed reactor, preparing a culture medium, inoculating, culturing by fermenting, detecting microbial content, collecting fermentation liquor and the like. According to the fermentation method, fiber materials are successfully arranged inside the reactor as carriers of immobilized lactobacillus cells, and the number of the lactobacillus cells can be substantially enhanced in fermentation of lactobacillus with the fibrous bed and under specific fermentation conditions. The biological reactor can be separately used or be taken as a seed fermentation tank to be combined with a common reactor for carrying out sequence fermentation in batches or continuous fermentation, and has the characteristics of being high in production efficiency and stable in batch production, shortening a fermentation period, ensuring high cell density and the like; fiber materials used by the method are cheap; the device is simple and convenient to operate, the production cost is low, and the fermentation method can be well applied to industrialized production of cells of lactobacillus such as lactobacillus acidophilus, lactobacillus bulgaricus and bifidobacterium.

The invention discloses bacillus subtilis, a fermentation method, powder and application. The bacillus subtilis is bacillus subtilis Gv-1, the depository authority is the China Center for Type Culture Collection, the depository address is Wuhan University, Wuhan of China, the depository number is CCTCC NO: M2015766, and the depository date is December 21, 2015. According to the invention, Kosteletzkya virginica earthnut endophyt is separated from a Kosteletzkya virginica earthnut for the first time, and purification, 16S rDNA sequencing and morphological and physiological characteristic analysis are performed, so that isolated and cultured stra Gv-1 is identified as bacillus subtilis. According to the invention, bacillus subtilis powder is prepared through bacillus subtilis fermentation liquor and is added into pig feed, and results show that the pig feed with the bacillus subtilis powder added can sufficiently realize the effect of the bacillus subtilis Gv-1, promote the production performance of piglets and digestive absorption for protein, Ca and P by the piglets, improve the intestinal microorganism flora of the piglets, and ensure that the intestinal microorganism flora is in an optimum equilibrium state.

The invention provides a streptomyces microflavus solid fermentation medium and a preparation method thereof, a streptomyces microflavus fermentation method and fermentation product, bio-control products of the fermentation product as well as application of the fermentation product and bio-control products, and relates to the technical field of microbial fermentation. The streptomyces microflavussolid fermentation medium is prepared from the following components in parts by weight: 40-50 parts of bran, 15-25 parts of rice hull, 25-35 pats of rice bran, 3-7 parts of calcium carbonate, and water. The streptomyces microflavus fermentation method comprises the steps of optionally enabling a strain of streptomyces microflavus to be subjected to seed activation and liquid seed culture, and performing fermentation culture by using the streptomyces microflavus solid fermentation medium to obtain the fermentation product. The streptomyces microflavus fermentation method overcomes the defect that the existing streptomyces microflavus is less in viable count and sporulation quantity. When being used for solid fermentation culture, the streptomyces microflavus solid fermentation medium is short in fermentation cycle and more in viable count, thus being suitable for industrial production; the obtained fermentation product has a significant effect in the aspect of controlling bacterial wiltof crops, thus increasing the quality and yield of the crops.

The invention relates to space-induced efficient bacillus natto, an application of bacillus natto and a preparation method of a troche of the bacillus natto. For efficient natto production strains obtained by conventional induction, a large batch of original strains needs to be induced, and strain variation amplitude sieved is small, so that the fermenting yield is hard to improve in a large scale. The bacillus natto (Bacillusnatto) S7-T3 provided by the invention can be applied to preparing medicines for reducing blood fat and blood pressure and health-care products. The enzyme generating activity and multiplication capacity of the bacillus natto provided by the invention are greatly improved, and the bacillus natto can be stably inherited to 30 generations without degeneration, so that glucose can be better utilized to generate non-acidic or neutral end products to decompose sulfur-containing amino acids. The space-induced efficient bacillus natto can be applied to preparing health-care products and medicines, and in particular applied to preparing health-care products and medicines for reducing blood fat and blood pressure.

The invention relates to the field of preparation of crop fertilizer and particularly relates to a compound microorganism fertilizer and a preparation method thereof. The compound microorganism fertilizer comprises 10-16 parts of molasses, 11-15 parts of urea, 10-15 parts of ammonium nitrate, 8-16 parts of calcium magnesium phosphate, 5-10 parts of potassium chloride, 5-8 parts of soybean meal, 6-12 parts of lignin, 8-12 parts of chitin, 8-12 parts of cellulose, 2-5 parts of lactobacillus fermentation liquid, 2-5 parts of yeast fermentation liquid, 2-5 parts of actinomycetes fermentation liquid and 2-5 parts of filamentous bacteria fermentation liquid. The compound microorganism fertilizer can be used for promoting plant absorption, is capable of improving the yield of plants to a great extent while avoiding fertilizer waste, and can guarantee soil fertility.

The invention discloses a solid-phase microecology preparation used for treating alcoholic liver injuries, and a preparation method thereof. According to the preparation method, the solid-phase microecology preparation is prepared by following steps: bifidobacterium longum NQ-1501 bacterial strains are made into bacterial powder; and the bacterial powder is mixed with auxiliary materials. The solid-phase microecology preparation is capable of treating alcoholic liver injuries, adjusting intestinal floras, and mitigating damages on human body caused by alcohol.

The invention discloses a preparation method of a microecological agent capable of reducing human body serum cholesterol content. The microecological preparation can be stored for 12 months in an ambient temperature condition. The method comprises the steps of preparing bifidobacterium longum NQ-1501 bacterial strain into bacterial powder, and then mixing the bacterial powder and auxiliary material into the preparation. The preparation can achieve the purposes that only cholesterol is reduced and the absorption of other nutritious substance is not affected, and overcomes the disadvantage that microecological preparation can not be stored in low temperature condition.

The invention discloses a pig fattening biological complete feed taking persimmons as base materials and a production process thereof. The production process comprises the following steps: fermenting by taking cheap persimmons as main raw materials, adding other carrier materials such as grain byproducts, namely bean pulp, rice bran and wheat bran, and adding microorganism fermenting bacterial florae suitable for animals to obtain a brand-new antimicrobial-free fermented feed. The production process has the advantages that the market vacancy is filled up, the resource advantages of the persimmons are utilized, the added value of the persimmons is improved, the cultivation enthusiasm of fruit farmers is enhanced, the local economy development is promoted, the raw materials of the biological feed are mixed according to the nutritional value of the pig fattening feed, strains are artificially cultivated, high-temperature-resistance strains are added, the total number of viable bacteria is high after a drying process, the production cost is low, and the obtained biological complete feed is comprehensive in nutritional value, high in nutrition, high in fattening speed, good in meat quality and especially suitable for pig fattening.

A process for treating the waste alcohol liquid from sugar refinery includes such steps as mixing it with cane pith filtered mud and powdered coal ash, stirring, adding yeast, bacteria and actinomycetes and fermenting to obtain solid fertilizer, or adding Japanese EM bacteria to said waste liquid and fermenting to obtain liquid fertilizer. Its advantages are low cost, high fertility and no secondary pollution.

The invention aims to provide a microecological preparation capable of preventing and treating reproductive tract infection of a dairy cow. Dominating lactic acid bacteria in the vagina of a healthy dairy cow are separated and identified; then two optimal lactic acid bacteria are selected; the two lactic acid bacteria are non-toxic according to results of safety detection on mice and have striking antagonistic effects according to results of detection on their antagonism on pathogenic bacteria. The two lactic acid bacteria are used as raw materials and fermented to prepare bacterial powders, then the bacterial powders of the two lactic acid bacteria are mixed together, and an obtained mixture is returned to the body of an ill animal; colonization of bacterial strains is benefited, an inhibitory effect is exerted on pathogenic bacteria, damage of chemical medicines to a body is avoided, the problem of residual medicines in the body is overcome, and the dairy cow is benefited in production of a dam.

The invention discloses a preparation method of a mushroom bran immobilized microbial agent. The preparation method comprises steps as follows: (1) preparation of a bacterial suspension: hydrocarbon degradation bacteria are added to normal saline to be prepared into the bacterial suspension; (2) preparation of a carrier: air drying is performed after mushroom bran is washed clean, and a product issieved by a 20-80-mesh sieve for later use; (3) adsorption: the mushroom bran in the step (2) and the bacterial suspension in the step (1) are mixed in the proportion of 1:(15-25) (v / v), the mixtureis put in a shaking table at the temperature of 30-37 DEG C at the speed of 120-160 rpm to be adsorbed for 16-24 h, and an upper solution is discarded; (4) centrifugation: the adsorbed mushroom bran is transferred to a centrifugal tube with normal saline, and a liquid supernatant is discarded through centrifugation; the centrifugation operation is repeated multiple times, and precipitates obtainedafter centrifugation are the immobilized microbial agent. The prepared mushroom bran immobilized microbial agent can be applied to remediation of petroleum-polluted soil, has high removal efficiencyfor petroleum hydrocarbon, causes no secondary pollution and has the remediation and degradation rate substantially increased as compared with independent mushroom bran and microorganisms.

The invention discloses an animal selenium-enriched health-care nutritional liquid, a preparation method and an application. The preparation method of the animal selenium-enriched health-care nutritional liquid comprises breeding of a selenium-enriched yeast strain, acquisition of a yeast hydrolysate, fermentation of a single probiotics strain, preparation of a probiotics mixed liquid, and compounding of the selenium-enriched yeast hydrolysate and probiotics. The preparation method specifically comprises the following steps of: adding selenium-enriched yeast hydrolysate dry powder into the probiotics mixed liquid in a proportion of 10-15g / L; and adding a fresh culture medium into the compound mixed liquid, adding potassium sorbate into the probiotics mixed liquid, the mass concentration of which is 0.08-0.1%, into the probiotics mixed liquid, and uniformly mixing the mixture to obtain the animal selenium-enriched health-care nutritional liquid. According to the animal selenium-enriched health-care nutritional liquid provided by the invention, five probiotics strains are separately fermented and are combined according to the optimum proportion, and the probiotics strains are synergistic, so that the animal selenium-enriched health-care nutritional liquid has the characteristics of novel strain combination, stable structure, high enzyme activity, great viable count and etc. Meanwhile, by adding the hydrolysate of bred highly selenium-enriched yeast into the nutritional liquid, and the hydrolysate and probiotics are organically combined, so that the growth-promoting and life-preserving effects of organic selenium and probiotics are exerted.

The invention discloses a production method of saccharomycopsis fibuligera. The method comprises the following steps: (1) preparing a liquid seed culture medium: obtaining the optimal liquid seed culture medium for cultivating denitrifying bacteria through a culture medium optimizing experiment; (2) preparing seeds: performing conventional bacteria screening and activation, and performing cultivation in the liquid seed culture medium to obtain liquid seeds in a logarithmic phase; (3) preparing a solid flat plate: performing a solid flat plate culture medium containing liquid culture medium components; (4) performing amplification fermentation: spraying the liquid seeds obtained in the step (2) on the surface of the solid flat plate and performing closed fermentation cultivation in a fermentation chamber; (5) collecting bacterial sludge; (6) preparing bacterial powder. By the production method, the saccharomycopsis fibuligera can be produced on a large scale, the saccharomycopsis fibuligera has a large number of viable bacteria, the production process is low in operation difficulty, the whole utilization rate of raw materials is high, the production cost is low and the production efficiency is high.

The invention provides a low-temperature compost inoculant which comprises pichia pastoris VT-21 with the cell concentration ranging from 1.0*108 cfu / mL to 5.0*109 cfu / mL, bacillus psychrphilus H H with the cell concentration ranging from 1.0*108 cfu / mL to 5.0*109 cfu / mL, pseudomonas spp. B5-16 with the cell concentration ranging from 5.0*108 cfu / mL to 1.0 *1010 cfu / mL and pseudomonas spp. B6-15 with the cell concentration ranging from 5.0*108 cfu / mL to 1.0 *1010 cfu / mL. According to the low-temperature compost inoculants, through optimum combination of bacteria, culturing and matching according to suitable active units are conducted, the obtained inoculant is large in total variable bacteria number, high in biological activity, good in synergistic effect, capable of enabling organic material compost to rise in temperature quickly in a low-temperature region, and compost products are stable in quality and high in fertilizer efficiency.

The invention relates to the technical field of fertilizers, in particular to a preparation method for a liquid fertilizer. The method comprises the following steps that S1, malt and spinach are crushed separately and then are uniformly mixed, distilled water is added, soaking is carried out, a pretreatment solution is added, and stirring is carried out at normal temperature so as to obtain a fermentation raw material, wherein the pretreatment solution is an alkaline solution; and S2, saccharomycetes is added into the fermentation raw material, anaerobic fermentation is carried out, filteringis carried out, and a filtrate is evaporated and concentrated so as to obtain the liquid fertilizer. The method has the advantages of being simple in process, safe and environmentally friendly, and the prepared liquid fertilizer has the advantages of being rich in nutrition, high in quality, long in fertilizer efficiency, high in nutrient utilization rate, capable of effectively promoting the cropgrowth and capable of increasing the crop yield.

The invention belongs to the technical field of food processing, and particularly discloses a method for direct feeding fermentation production of instant preserved cowpeas. The method for direct feeding fermentation production of instant preserved cowpeas comprises the following steps of 1, processing raw materials, wherein fresh, crispy and tender cowpeas with compact tissue and without plant diseases or insect pests are picked, washed and drained for use; 2, fermenting, wherein the cowpeas are placed into a fermentation jar, auxiliary materials, carbon sources and direct feeding leavening agents are sequentially added and mixed to be uniform, then, fermentation is performed, and the instant preserved cowpeas can be obtained; the direct feeding leavening agents include leuconostoc mesenteroides, bacillus subtilis, bifidobacterium breve, Lactobacillus curvatus and bacillus pumilus. According to the method for direct feeding fermentation production of instant preserved cowpeas, the specially manufactured direct feeding leavening agents are adopted as zymocyte to be mixed with cowpeas for fermentation, the fermented instant preserved cowpeas are high in sensory quality, the food safety is good, and the edible value is high.

The invention relates to a microcapsule containing an active substance and a preparation method thereof, in particular to a multilayer microcapsule containing an embedded active substance and a preparation method of the multilayer microcapsule. According to the application, the microcapsule particles are prepared by a method of combining extrusion rolling core making (or centrifugal granulation core making) with fluidized spray coating; the preparation method comprises the following steps: firstly, preparing a primary pellet core by adopting an extrusion spheronization granulation method or a centrifugal granulation method, and then uniformly fluidizing a water-blocking coating layer material and a hydrophilic coating layer material to the surface of the pellet core. The prepared microcapsule particles are safe, free of toxic and side effects, small in particle size, rich in active substances and good in stability under the normal temperature condition, and have a slow release effect.

The invention discloses a fermented tea and a preparation method thereof. The fermented tea comprises the following raw materials in parts by weight: 2-8 parts of fermented jerusalem artichoke powder and 92-98 parts of fermented tea leaves. The fermented jerusalem artichoke powder and the fermented tea leaves are uniformly mixed so as to obtain the fermented tea. The fermented jerusalem artichoke powder and the fermented tea leaves are obtained by adopting microbial fermentation so as to remove heavy metal residues and pesticide residues. Cooperation between tea polyphenol and the jerusalem artichoke powder is utilized to reduce the body weight and liver weight of hyperlipidemia patients. The content of high-density lipoprotein cholesterol is raised to clear accumulated cholesterol on artery vessel walls, and reduce the contents of triglyceride and total cholesterol so as to realize the object of decreasing blood lipid. A combination of the tea polyphenol and the jerusalem artichoke powder also has a strong effect of inhibiting converzyme activity so as to play an effect in reducing blood pressure. The combination of the tea polyphenol and the jerusalem artichoke powder has an effect of adjusting glycometabolism disorders of human bodies, and can lower blood sugar levels so as to effectively prevent and treat diabetes.

The invention discloses a compound probiotic functional food used for regulating immune response to enteritis in children. The compound probiotic functional food used for regulating immune response toenteritis in children comprises bifidobacterium lactis, lactobacillus rhamnosus, bifidobacterium animalis, lactobacillus acidophilus, lactobacillus reuteri, lactobacillus fermentum, bifidobacterium breve, fructo-oligosaccharides and isomalto-oligosaccharides. A preparation method of the compound probiotic functional food used for regulating immune response to enteritis in children comprises the following steps: separately sieving ingredients of mixed probiotics and mixed prebiotics so as to ensure that the particle size is smaller than 80 meshes; weighing the sieved materials according to theproportion by mass; blending the weighed materials by using a dry blender so as to obtain a uniform mixture; and then, performing tablet compression on the mixture so as to obtain tablets, or directly weighing and bagging the mixture. The compound probiotic functional food used for regulating immune response to enteritis in children solves the problems of unsatisfactory regulation effects on enteritis and immune functions in children of existing compound probiotic functional foods due to few flora species and unbalanced flora proportion.

The invention relates to a recombinant lactobacillus rhamnosus engineering strain and a preparation method thereof. The lactobacillus rhamnosus engineering strain carries expression vectors of a catalase gene and a superoxide dismutase gene which are connected in series. The method for preparing the engineering strain comprises the following steps of: 1) amplifying catalase gene segments and superoxide dismutase gene segments from genomes of lactobacillus sake and streptococcus thermophiles by a polymerase chain reaction (PCR) method; 2) designing a specific restriction enzyme site, wherein the restriction enzyme site is connected in series with an escherichia coli-lactobacillus shuttle plasmid vector pSIP502; and 3) transforming a recombinant plasmid into lactobacillus rhamnosus by an electroporation method. The recombinant lactobacillus rhamnosus engineering strain prepared by the method can remarkably enhance oxidation resistance and can be applied to the fermented food industry.

The invention belongs to the field of agricultural biotechnologies, and particularly relates to a method for preventing and treating verticillium wilt of cotton verticillium wilt seriously diseased fields. The method includes that disease-tolerant varieties, microbial organic fertilizers and resistance inducers are jointly used, national or provincial-level authorized cotton varieties with verticillium wilt disease indexes lower than 30 are adopted, bacterial strains CEF08111 are fermented to obtain the microbial bacterial fertilizers, the application amounts are 4 m<3>, and basal applicationis carried out; the resistance inducers are a type of exogenous biological or non-biological substances capable of inducing cotton plants to develop resistance to target diseases. Application processes include carrying out leaf surface spraying in pathogenic initial phases. The method has the advantages that the method is green, environmentally friendly and sustainable and is high in efficiency, synergistic control effects can be realized for the cotton verticillium wilt, and yield loss due to diseases can be remarkable abated.

The invention belongs to the field of microecological preparations, and particularly relates to preparation and application of an efficient immunoregulation micro-ecological preparation. The preparation method comprises the following steps of: S1, preparing a lactobacillus acidophilus fermentation culture medium from the following raw materials in percentage by weight: 0.5-1.0% of glucose, 0.1-0.5% of isomaltooligosaccharide, 0.1-0.5% of soy peptone, 0.1% of diammonium hydrogen citrate, 0.1% of sodium acetate, 0.1% of K2HPO4, 0.015% of MgSO4, 0.02% of MnSO4, 0.025% of L-cysteine hydrochloride, 0.01-0.05% of polysaccharopeptide, 0.01-0.05% of astragalus polysaccharide, 0.01-0.05% of triterpenoid saponin and water; S2, adding lactic acid or NaOH into the lactobacillus acidophilus fermentation culture medium to enable the pH range of the lactobacillus acidophilus fermentation culture medium to be 6.0-6.4, preparing a fermentation culture medium, and finally, conducting sterilization for 30 min through high-pressure steam at the temperature of 121 DEG C; and S3, cooling the fermentation culture medium obtained in S2 to 37 DEG C by using brine ice. The total number of viable bacteria of lactobacillus acidophilus can be remarkably increased, and meanwhile, the inhibition effect on harmful bacteria (escherichia coli and salmonella) is very remarkable.

The hotpot ingredient is prepared from the following raw materials in parts by weight: 10 to 12 parts of citrus peel, 4 to 6 parts of cordyceps sinensis, 8 to 10 parts of ramulus mori, 12 to 15 parts of maca and 10 to 15 parts of phellinus igniarius. The preparation method comprises the following steps: (1) selecting ripe oranges with uniform size, cleaning, opening the tops of the oranges, taking out the flesh of the oranges, and punching; (2) weighing; (3) mixing the citrus peel, the cordyceps sinensis and the mulberry twigs, performing ultrasonic extraction, and performing vacuum concentration on an extracting solution; and (4) slicing maca and phellinus igniarius, filling the sliced maca and phellinus igniarius into the hollow main body of the citrus, soaking in the active extract concentrated solution, sterilizing at high temperature, cooling, inoculating high-temperature-resistant probiotics, fermenting, taking out, covering a citrus top cover, drying at low temperature, taking out, and packaging. The hotpot ingredient capable of reducing uric acid and improving immunity is simple in preparation process and low in production cost, and has a good industrial application prospect.

The invention relates to a simple bacillus product identification method, in particular to a simple method for identifying activities of bacillus licheniformis and bacillus subtilis (comprising bacillus natto) in a bacillus product. The method comprises the following steps of: (1) activating the bacillus product; (2) culturing the bacillus product; (3) recording the temperature in the culture process of the bacillus product; and (4) judging the quality of the bacillus product. The method is easy to operate and low in requirements for a culture medium and culture conditions, and can be supplied to feed and breeding enterprises which do not have microbiological examination means or do not have sound microbiological examination means at present and used for identifying the bacillus activity of the bacillus product and guiding purchase and use of the bacillus product.

The invention relates to the technical field of fermented milk products, in particular to wild mushroom yoghourt and a preparation method thereof. The wild mushroom yoghourt is prepared by mixing andfermenting the following components in percentage by mass: 0.2-0.7% of a stabilizer, 0.3-0.8% of single cream, 6-9% of white granulated sugar, 0.1-0.5% of whey protein powder, 0.1-0.25% of wild mushroom powder, 0.5-0.8% of prebiotics and the balance of raw milk. Aiming at the defects in the prior art, the invention provides the wild mushroom yoghourt and the preparation method thereof, so that theyoghourt is high in acid yield and high in probiotic activity, and the quality and stability of the yoghourt are improved.

The invention discloses a purple potato, carrot and viable organism compound beverage made by fermentation liquor containing dumpling-type fermentation bodies. The compound beverage is characterized in that the compound beverage comprises, by weight, 100-120 parts of carrots, 20-30 parts of carrot tops, 8-10 parts of degreased cow milk, 12-14 parts of saccharose, 4-6 parts of citric acid, 1-2 parts of bifidobacterium, 1-2 parts of Angel yeast, 1.2-1.4 parts of cellulose, 0.8-1 part of peptone and a proper amount of water. The made purple potato, carrot and viable organism compound beverage is high in viable count, good in sensory quality and rich in nutritive value, foreign smell of the carrots is eliminated through the bifidobacterium, and the beverage tastes good, is large in viable count and rich in amino acid and multivitamin and has an important function on human bodies.