130 results about "High fertility" patented technology

The invention relates to an artificial reproduction method for schizothorax wangchiachii. The method mainly comprises the following seven steps: bait preparation, parent fish sourcing, parent fish domestication, parent fish selection, artificial insemination, fish egg incubation and fish fry feeding. The artificial reproduction method for schizothorax wangchiachii has the beneficial effects that oxytocin is not used, the schizothorax wangchiachii can lay eggs and produce sperms, and relatively high fertility rate and hatchability can be obtained; the problem that female and male wild fish do not develop or develop asynchronously in a pond culture environment is solved fundamentally; the damage on the parent fish of the schizothorax wangchiachii is reduced, and the cultivation cost is also reduced; the method is reasonable and efficient, the hatchability is improved, and the waste of fish egg resources is reduced; and the aberration rate of the fish fry is reduced while the survival rate of the fish fry is improved.

The invention relates to the technical field of seedling matrixes of crops and particularly relates to a rice seedling matrix and a preparation method thereof. The rice seedling matrix with good permeability and high fertility is particularly prepared from the main raw materials of crop straws, cow mature, rice husk, seed cakes, microbial zymophyte and the like through steps of material preparation, soaking, straw piling, straw composting, matrix preparation and the like. The rice seedling matrix comprises the following main raw materials in percentage by weight: 35%-45% of the crop straws, 27%-34% of the rice husk, 16%-24% of the cow mature and 7%-15% of seed cakes, wherein 1kg of the microbial zymophyte is used in each 5-6 cubic meters of the crop straws. According to the preparation method, the soil-free matrix production is realized, the utilization of non-renewable resources is reduced, the damage caused to farmland soil resources is reduced, the environment is protected, the factory seedling is realized, the cost is lowered, the plant diseases and the insect pests are reduced, the content of soil organic matters is increased, and the yield and the milled rice rate can be increased.

The invention relates to a method for inducing gonad maturity and ovulation of a verasper variegates parent fish by implanting a slow-release hormone, which comprises two aspects of cultivating an optimally selected parent fish and adjusting temperature and light to promote the gonad development of the parent fish, and implanting the slow-release hormone for promoting a metrocyte of the parent fish to reach final maturity and ovulation. In the invention, the slow-release hormone is a salmon gonadotropin-releasing hormone analog (sGnRHa) and is coated with a safe and high-efficiency polymer slow-release transitive system (95 percent of cholesterol and 5 percent of cellulose); a female parent fish with gonad in fourth stage is selected by using a living body sampling method or a light table method; a special implanting gun is used for implanting in back muscles, and the effect time of the slow-release hormone lasts for 14-18 days. The method can enable the metrocyte of the verasper variegates parent fish to achieve final maturity and ovulation, has low duress on parent fish reproduction, high rate of parent fish survival and gonad maturity, high fertility rate being 72 percent and high hatching rate being 56.2 percent, and can acquire a bulk of high-quality oosperms. The invention is a set of complete control technique method, can stably regulate and control the development, the maturity and the ovulation of the gonad of the verasper variegates parent fish, and acquire high-quality oosperms.

The invention discloses a method for inducing misgurnus anguillicaudatus gynogenesis tetraploid fries by using heterogenenos sperms. The method comprises the following steps: selecting parents, performing genetic inactivation on sperms, inducing gynogenesis tetraploid fries, and performing cold shock treatment to obtain gynogenesis tetraploid fries. The highest fertility rate, hatching rate and survival rate of the gynogenesis tetraploid misgurnus anguillicaudatus can reach 76.99+/-2.51%, 54.54+/-1.87% and 17.9+/-0.65% respectively. The method disclosed by the invention has the characteristics of advancement, high efficiency, practicability and reliability, and has an important application value and a wide application prospect in the breeding of misgurnus anguillicaudatus.

The invention discloses a preparation method of a high-efficient decay-promoting agent. The preparation method comprises the three steps of activation and purification of bacteria, enlarged culture of a liquid shaking table and preparation of solid bacterial powder, and comprises the concrete steps of inoculating fungi, actinomycetes and bacteria on respective culture media, and carrying out streak culture; picking single colonies of the bacteria and the actinomycetes or mycelia or spores of the fungi to inoculate into respective prepared liquid culture media, and culturing through the liquid shaking table; finally inoculating each bacterium of each liquid culture medium into a prepared culture material according to the proportion, dry-curing, mixing the dry-cured bacteria according to the proportion, and preparing the high-efficient decay-promoting agent with high fertility. The invention provides the preparation method of the high-efficient decay-promoting agent, which has the characteristics of capability of enabling the decomposing speed of straw or farmyard manures to be improved remarkably, high degradation ability, high decay promoting efficiency, and good effect.

The invention discloses an elite breeding method for apis cerana in the southern area of China. A swarm with good fertility performance and uniform shape feature can be kept as a population; by means of four-generation advantageous hybridization propagation, high-fertility of the apis cerana on Changbai mountains, disease-resistance of the apis cerana in Aba county, cold-resistance of the apis cerana in the northern area and heat-resistance of the apis cerana in Hainan province are taken into consideration, and accordingly the population, with good comprehensive characteristics, of the apis cerana in the southern area of China is bred; growth of the apis cerana in the southern area of China is stabilized, and the apis cerana in the southern area of China can still keep good characteristics after inheritance of multiple generations. Parents of good apis cerana are led in from different areas of China, by means of an advantageous hybridization propagation technique, the good population, with disease resistance, high-temperature and low-temperature resistance, high yield and high accumulated nutrition, of the apis cerana in the southern area of China is obtained after four-generation hybridization breeding, good characteristics of offsping of the apis cerana in southern area of China are maintained through concentrated scientific breeding and artificial breeding, and a southern apis cerana breeding technique is developed by summarizing bee breeding techniques and apis cerana breeding techniques in the southern area of China.

Provided are a diluent and a sperm preservation method using the diluent. The diluent is useful in preservation of sperm with high fertility, and is capable of stably achieving quality control and improving quality of the sperm. Refrigerating or freezing sperm using the diluent for sperm, which includes an aqueous solution containing at least one oligosaccharide selected from the group consisting of a fructo-oligosaccharide, an isomalto-oligosaccharide, a gentio-oligosaccharide, and a galacto-oligosaccharide, can improve the quality of preserved sperm and provide sperm having high fertility, at a reduced cost.

The invention provides a method for determining a core promoter of a miR-27a gene. According to the method, 8 miR-27a promoter deletion fragments are amplified from genomic DNA in mouse blood, recombinant dual-luciferase expression vectors from pGL3-D1 to pGL3-D8 are constructed, after transfer-in of CHO cells, luciferase activity of the 8 deletion fragments is detected, and a core promoter area is obtained; a core area of miR-27a is identified, and a new promoter resource is provided for genetic engineering and molecular breeding. The invention further provides a method for determining an internal transcription factor Myod binding site of the core promoter of the miR-27a gene. By means of overexpression of the transcription factor Myod and site-specific mutagenesis of the transcription factor binding site in the core promoter area, a mutant type fluorescent expression vector is constructed, luciferase activity is detected, and the transcription factor binding site is determined; basisis provided for research of applying expression regulation of miR-27a to high-fertility molecular regulation mechanism of livestock.

The invention discloses a high-fertility biofertilizer for crops, which comprises the following components in parts by weight: 50-60 parts of rice straw, 25-35 parts of corn stalk straw, 14-36 parts of soybean straw, 10-18 parts of potato straw, 21-35 parts of chicken manure, 10-15 parts of duck manure, 4-6 parts of ammonium monoacid phosphate, 3-8 parts of manganese hypophosphite, 0.01-0.02 part of Bacillus siliceous, 0.01-0.02 part of Bacillus cereus, 0.01-0.02 part of Bacillus thuringiensis, 25-30 parts of rape cake, 25-30 parts of chicken manure, 14-25 parts of rice bran and 10-16 parts of wheat bran. The formula disclosed by the invention is scientific and reasonable, achieves nutrient balance, and satisfies the nutrients required by plant growth; and the microbial inoculants are added to accelerate degradation of high-polymer natural organic compounds into the organic fertilizer, thereby achieving the goals of low input and high output, and further enhancing the economic benefit of the crops.

The invention relates to a high-fertility long-acting anti-verticillium-wilt biological bacterial fertilizer and a preparation method thereof. The biological bacterial fertilizer is formed by mixing bacterial fertilizer carrier grains and bacterium agent particles. Compared with that of a biological bacterial fertilizer which is commonly used in production, the fertilizer efficiency of the biological bacterial fertilizer is higher. By taking chilies, eggplants and tomatoes as fertilization objects and taking no fertilization, fertilization with an inorganic compound fertilizer and fertilization with an organic fertilizer, chicken manure, as contrasts, the biological bacterial fertilizer can be used for obviously promoting biological yields of the three vegetables, and the biomass of the three vegetables applied with the bacterial fertilizer is more than 50% higher than that of vegetables which are not applied with fertilizer, applied with the inorganic compound fertilizer and applied with the organic fertilizer, chicken manure. A test on eggplants inoculated with verticillium wilt shows that the bacterial fertilizer can be used for effectively inhibiting and relieving the diseases. A soil enzyme activity test shows that the enzyme activity of soil treated by the biological bacterial fertilizer is obviously higher than that of soil without fertilization and that of soil applied with the inorganic compound fertilizer or the organic fertilizer, chicken manure; nitrogen transformation, phosphorus transformation and organic matter transformation capabilities of soil can be facilitated well.

The invention discloses a rice straw strip-shaped deep ploughing returning wheat planting method. The method comprises the steps of rice straw strip-shaped sorting, rice straw strip-shaped deep ploughing returning, shallow ploughing and field block leveling, wheat strip-shaped sowing and the like. The method has the advantages that firstly the rice straw deep ploughing returning contributes to control of spread and damage of germs and eggs carried by the straws in field; secondly the wheat strip-shaped planting at the field block which is not deeply ploughed, so that the soil at the root layer of the wheat has higher fertility and full seedling and strong seedling are facilitated; thirdly the strip-shaped ploughing can obviously reduce the mechanical energy consumption, and the straw deep ploughing strips are rationally switched year by year, the soil plough horizon can be deepened, and the soil fertility is improved.

The present invention discloses an artificial breeding method for Triplophysa xichangensis, and Triplophysa xichangensis belongs to a plateau cold water fish. The present invention provides a selection method, cultivation method and artificial spawning method for parent fishes of Triplophysa xichangensis, and a method for feeding a seed first feed or the like. The method includes the steps: under an artificial controllable condition of water quality and cultivation, employing two doses of oxytocic including luteinizing hormone releasing hormone A2(LHRH-A2) and human chorionic gonadotropin (HCG) on the parent fishes, and allowing gonad development of female fishes and male fishes to be substantially synchronous; and employing natural spawning insemination, and taking the cooked egg yolk as the seed first feed. The method has the high induced spawning rate, the high fertility rate, the high hatching rate and unanimous seedling emergence, the induced spawning rate is 100%, the fertility rate is higher than 95%, the hatching rate is in a range from 75% to 95%, and the survival rate is higher than 95%. The practical applications prove that the method is low in cost, is simple to operate, is highly operable, and is suitable for large scale production of Triplophysa xichangensis.

The invention provides an organic planting method for loofah, belonging to the technical field of agriculture, and specifically relates to a planting method for the loofah. The invention provides the organic planting method for the loofah with little pesticide residue. The organic planting method for the loofah is characterized by comprising the following steps: 1, a manner of direct sowing or seedling transplanting of the loofah can be adopted, and no matter which manner is adopted, the better manner is to soak seeds and accelerate germination at first and then sow seeds; 2, nutrient and water management, wherein stem vines of the loofah have large growth amount, bear well and need a great amount of water and nutrients; 3, frame building, vine introducing and vine binding: timely building a frame after a second deep intertillage, wherein a shed frame, in general, is better to be adopted for varieties with long vines which grow vigorously and have strong branching capacity; 4, soil preparation and field planting: selecting soil with strong water-retaining capacity and high fertility for cultivation of the loofah, carrying out deep ploughing and soil drying under the sun before planting, and applying about 5000 kg of farmyard manure per mu; and 5, plant arrangement, wherein the loofah is a vining plant, so the frame is needed to be built for cultivation; generally, when the vines of the loofah have a length of 30 to 60 centimeters, the frame is needed to be built; and the form of the frame can be determined according to the varieties adopted for cultivation, plant growth capacity and branching conditions.

A composite pesticide for rice plantation is provided and belongs to the technical field of pesticides. The composite pesticide is prepared from rapeseed cakes, rice waste, bentonite, a nitrogen-phosphorus-potassium compound fertilizer, composite amino acids, trace elements, licorice segments, humic acid, clover, Chinese gentian and an insecticide. The insecticide is prepared from, by weight, 10-30 parts of mock lime flower, 10-30 parts of rhizoma coptidis, 5-20 parts of temple juniper, 5-20 parts of pilea longicaulis Hand.-Mazz., 5-20 parts of rhododendron spinuliferum, 5-15 parts of inula flower, 3-12 parts of vernonia anthelmintica (L.) Willd., 3-12 parts of elm bark and 3-12 parts of calamus. Raw materials of the composite pesticide are synergistic. The prepared composite pesticide has high fertility and obvious antibacterial capability, can obviously reduce the disease rate of rice, and is efficient, time-saving and labor-saving.

The invention discloses a technique for manufacturing an active phosphorus organic fertilizer from pig manure, which comprises the following steps: raw material pretreatment, anaerobic fermentation, aerobic fermentation and aging. The weight percent of the raw material pig manure on dry basis is 70-95%. An auxiliary material with the organic content of higher than 60% is utilized to regulate the total water content of the pig manure and auxiliary material to 50-70%; an organic matter decomposing inoculant is added until the effective viable count in the total material is up to 0.5 million/g; and in the aerobic fermentation period, a phosphorous acidic matter is utilized to regulate the pH value to be greater than or equal to 5.5 and smaller than or equal to 8.5. The technique can effectively treat the cultivation farm excrement, enhances the index stability of the fertilizer, greatly reduces the effluvium generated in the fertilizer production process, and enhances the phosphorus utilization ratio and humus content. The active phosphorus organic fertilizer has the advantages of high thermal insulation, high fertility retention and high air permeability, contains more activated phosphorus element, and thus, has excellent promotion effects on crop root system growth, seed development and the like.

The invention discloses a method for gradually replacing tobacco nitrogen fertilizers with multicropping vetches. The method comprises the following steps of hole-sowing a row of Yunguang early vetches on each of two sides of tobaccos on a tobacco planting ridge, and cradling, chopping, turning and pressing fresh Yunguang early vetches in early March of the next year at the period of full bloom, wherein the using amount of low- and medium-fertility soil is 15,000 kg/hm<2>, and the using amount of high-fertility soil is 7,500 kg/hm<2>; aftercropping the tobaccos, and continuously multicropping the tobaccos and the vetches. Compared with the using amount of conventional fertilizer application, the using amount of tobacco nitrogen fertilizers is reduced by 15 percent in the first year and is progressively decreased year by year on the basis of the using amount of the last year according to 15 percent until the using amount of the nitrogen fertilizers is progressively decreased to zero. According to the method, the using amount of the tobacco nitrogen fertilizers is greatly reduced; furthermore, the yield of tobacco products can be obviously improved and is increased by over 10 percent; the quality of the tobacco products is obviously improved; the output value is increased by over 14 percent; the effect of improving the soil is outstanding; the average annual increase of organic matters in the soil is 0.025 percent; the average annual decrease of the volume weight of the soil is 0.011 g/cm<3>.

A method for promoting ovary maturity and oviposition for Japanese prawn, relating to a method for breeding prawn, in particular to a method employing exo-genetic hormone. It comprises following steps: preparing 5-hydroxytryptamine solution, employing sterilized sea water as dissolvent, with the content ratio of them being: 1 mg:1-5ml, injecting every prawn at least one time with dose being 2-60 2~60mug/g of the 5-hydroxytryptamine according to parent prawn's weight, injecting 1-3 times for every prawn with time interval being 5-10 days. It is unnecessary to extract eyestalk, and no damage to reproductive endocrinology organ, and thus the invention is characterized by short ovary mature time, low prawn mortality, high fertility and hatchability rate, easy to operate and low cost, and etc.

The invention belongs to the technical field of ornamental chrysanthemum breeding, and especially relates to an ornamental chrysanthemum potted cultivation method. The ornamental chrysanthemum potted cultivation method specifically comprises the following steps: 1) using wild chrysanthemum for grafting breeding; 2) using a ridge method to culture ornamental chrysanthemum seedlings; 3) transplanting chrysanthemum seedling potted plant; 4) culturing seedling trunks; 5) pinching and picking buds; 6) shaping and remaining flowers; 7) regulating flowering time; 8) performing flower bud management. The method uses a grafting rapid propagation technology to breed ornamental chrysanthemum, excellent characteristics of an original variety is maintained, and survival rate of breeding of the ornamental chrysanthemum is improved. Cultivation soil has relatively high fertility and good hydrophobic property, and satisfies requirements of fertilizer and moisture and prevents a water logging disaster. Through short-day treatment on the ornamental chrysanthemum, flowering time is promoted to bring forward. Through KT treatment, chlorophyll, nucleic acid, and protein contents of the ornamental chrysanthemum are reduced, cellular structure is protected, and flowering time of the ornamental chrysanthemum is prolonged.

The invention discloses a method for preparing a fertilizer from mixed animal manure, which comprises the following steps: mixing mixed animal manure, calcium oxide and water, regulating the pH value, and adding 0.5 part of effective microorganisms to obtain a mixture; regulating the pH value to 8, adding neutral proteinase, and carrying out enzymolysis; steaming the mixture at 120 DEG C under 1.5 atmospheric pressures; pulverizing the mixture four times; putting a seaweed-containing sealed box in the mixture, standing for 15 minutes, and taking out the box; regulating the pH value of the mixture to 5, adding lactobacillus, and carrying out anaerobic fermentation; adding yucca, fructus momordicae, oyster shell, effective microorganisms and straw feed ferment into the mixture, and carrying out anaerobic fermentation; adding a manure ferment into the mixture, and carrying out anaerobic fermentation; and steaming the mixture at 90 DEG C under 1.2 atmospheric pressures for 5 minutes, relieving to one atmosphere, cooling the mixture to 15 DEG C, and adding 0.005 part of nano carbon into the mixture. The fertilizer prepared by the method has high fertility, and is free of malignant bacteria and pests.

The invention discloses a lemon grass organic fertilizer and a preparation method thereof, belonging to the technical field of agricultural fertilizers. The organic fertilizer comprises the following raw materials in parts by weight: 30-40 parts of lemon grass waste residue, 20-30 parts of activated sludge, 15-20 parts of candy pomace, 17-21 parts of chicken manure, 10-20 parts of vinasse and 5-15 parts of tea bran. The preparation method is implemented by rotting and fermenting the raw materials in a dark place. The method converts various wastes into the biological fertilizer by fermentation, thereby solving the problem of environment pollution caused by waste discharge, and changing wastes into valuable substances. The organic fertilizer can improve the soil structure and kill pests in soil, has the advantages of high fertility and endurable fertilizer efficiency, and improves the quality of agricultural products.

The invention discloses a fertilizer used for fruit trees. The fertilizer comprises, by weight, 50-100 parts of straw powder, 60-90 parts of animal manure, 20-40 parts of plant ash, 20-40 parts of acrylic acid, 10-20 parts of diammonium phosphate, 10-30 parts of potassium dihydrogen phosphate, 0.5-4 parts of sodium tetraborate, 0.2-3 parts of boric acid, 0.5-6 parts of sodium glutamate, 0.1-5 parts of sodium alginate, 1-5 parts of amino acids, 0.2-4 parts of cellulose and 1-5 parts of medium and trace elements. Organic components and inorganic components are mixed, and all the components have a synergistic effect, so the fertilizer has high fertility, and meets nutrition required by growth of the fruit trees.

The invention relates to a Sophora alopecuroide and Cynanchum komarovii composite organic fertilizer and a preparation method, which belongs to the organic fertilizer technical field. The organic fertilizer is prepared by Sophora alopecuroide, Cynanchum komarovii and straw (corn, wheat, rape and the like)compost according to certain ratio, each component has the following weight percentages of 0-40%, 0-40% and 20-60% respectively; the preparation method comprises the following steps: mixing Sophora alopecuroide, Cynanchum komarovii and straw compost according to certain ratio and sequence for granulation, drying and packaging, wherein water is added for retting Sophora alopecuroide and Cynanchum komarovii for 6-10 days before granulation, and the straw compost is prepared by straw, night soil and water according to weight ratio of 10:3:2 for oxygen consumption fermentation and decomposing for 20-30 days. According to the invention, the Sophora alopecuroide and Cynanchum komarovii having antibiosis, pest-resistance and nutrition are taken as materials, by organically compositing with the straw compost, the organic fertilizer has the characteristics of wide raw material source, low cost, green performance, natural performance, environmental protection, high fertility and the like, so that the insect damage can be effectively prevented, the disease can be reduced, and the composite organic fertilizer has substantial effect for planting and producing the organic agricultural products (grains, vegetables and fruits).

The invention discloses a manufacturing method of a soilless culture substrate. The method comprises the steps of preparing raw materials, mixing and crushing the raw materials in a certain proportion, performing composting and fermentation, controlling the water content to be 20-30%, controlling the temperature to be 55-70 DEG C, turning and throwing according to needs, mixing a compound fertilizer into a backing material subjected to composting, performing mature fermentation again, performing sampling detection after completing mature fermentation, packaging and delivering when qualified, and reprocessing as the raw materials when not qualified. By virtue of the mode, the problems of incomplete biomass decomposition can be effectively solved by adopting the manufacturing method of the soilless culture substrate; the manufacturing method disclosed by the invention has the advantages of stable product quality, little environmental pollution, high fertility, good seedling growth effect, wide material source, cheap price and the like, and has broad market prospects in agricultural markets.

The invention relates to a multifunctional peanut leaf fertilizer which comprises the following components in percentages by weight: 0.95-1.55% of macroelements, 0.25-0.45% of trace elements, 0.75-1.25% of organic monosaccharide, 0.35-0.55% of a pesticide, 0.15-0.25% of a wetting agent and the balance of water. The macroelements are a salt containing any one or more of nitrogen, phosphorus and potassium; the trace elements are a salt containing any one or more of iron, copper, zinc, cobalt, manganese, chromium, selenium, iodine, nickel, fluorine, molybdenum, vanadium, tin, silicon, strontium, boron, rubidium and arsenic; the organic monosaccharide is any one or more of glucose, fructose and galactose; the pesticide is one or two of chlorpyrifos and chlorothalonil; the wetting agent is a detergent. By separately applying the multifunctional peanut leaf fertilizer provided by the invention to field blocks with low fertility and high fertility, growth and development of peanuts are obviously promoted; the ability of photosynthesis of the peanuts is obviously enhanced, the output and milled rice rate are increased, and the quality indexes, for example, proteins and fat, of the peanuts are obviously improved.

The invention relates to a nitrogen fertilizer applying method, and the method is as follows: a quantitative nitrogen fertilizer is respectively applied to rice in the seventh day, the fourteenth day, the thirty-fifth day, the forty-ninth day, the fifty-sixth day, the seventieth day and the seventy-seventh day after rice transplanting so as to improve rice yield and / or nitrogen utilization rate. The method has the advantages of timing and quantitative nitrogen fertilizer application and simple and easy operation. Compared with a real-time nitrogen management mode and a leaf age nitrogen application mode, the nitrogen fertilizer applying method can synchronously significantly improve the rice yield and the nitrogen utilization rate in a low fertility field, the nitrogen utilization rate in a medium fertility field of the nitrogen fertilizer applying method is higher than that of the leaf age nitrogen application mode, and the rice yield in a high fertility field of the nitrogen fertilizer applying method is higher than that of the real-time nitrogen management mode.