Four-strain mating pair-hybridization breeding method for litopenaeus vannamei

A four-line matching, double-hybrid technology, applied in the field of aquatic animal genetics and breeding, can solve problems affecting downstream seedling breeding, breeding, processing industries, price increases, and raising breeding costs, so as to speed up the progress of breeding, increase profits, and breed survival rate-enhancing effect

Inactive Publication Date: 2018-12-21
渤海水产育种(海南)有限公司
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AI Technical Summary

Problems solved by technology

[0003] With the expansion of the breeding scale, the demand for high-quality seedlings is becoming more and more urgent. my country's self-selected varieties have achieved good results, but the current varieties still cannot meet the requirements of my country's Litopenaeus vannamei farming industry for new varieties and high-quality parent species. Shrimp demand, more than 300,000 pairs of broodstock still need to be imported every year
The excessive dependence on imports of broodstock has also brought about a series of problems: (1) The price of imported broodstock has been increasing year by year. Cutting off the supply of broodstock to the domestic market will seriously affect the entire downstream nursery, breeding, and processing industries

Method used

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Embodiment Construction

[0016] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0017] (1) Establishment of breeding base group:

[0018] Collect the germplasm materials that have entered the Chinese market with excellent traits and have obvious adaptability after multiple generations of local breeding as the basic breeding group. The breeding base group is separated into multiple breeding base groups according to the source pool, and the parent size of each breeding base group is greater than 1000 pairs.

[0019] (2) Strain breeding

[0020] Pedigree construction was carried out for each breeding base group. Record the egg production and hatching rate of the parents of each family to evaluate the reproductive performance of the parents. The size of the larvae raised by each family should not be less than 50,000. Different families are cultured under the same conditions. The growth traits of different families , survival rate traits and fecund...

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Abstract

The invention relates to a four-strain mating pair-hybridization breeding method for litopenaeus vannamei, and belongs to the field of genetic breeding for aquatic animals. The method specifically comprises the steps of collecting breeding materials, and constructing four specialized strains including a fast-growth strain (strain A), a high survival / high propagation strain (strain B), a high survival / fast growth strain (strain C) and a high propagation strain (strain D); conducting primary hybridization testing between the fast-growth strain (strain A) and the high survival / high propagation strain (strain B) to select an optimal hybridization combination (AB) with high growth speed; meanwhile, conducting primary hybridization testing between the high survival / fast growth strain (strain C)and the high propagation strain (strain D) to select an optimal hybridization combination (CD) with high fertility; conducting secondary hybridization testing between the optimal hybridization combination (AB) with high growth speed and the optimal hybridization combination (CD) with high fertility to screen out an optimal mating combination (ABCD) to serve as commercial young litopenaeus vannamei. The finally-cultivated strain has the advantages of being high in growth speed and survival rate, and shows obvious yield strengths in different breeding modes.

Description

technical field [0001] The invention relates to the field of genetic breeding of aquatic animals, in particular to a four-line matching double-hybrid breeding method of Litopenaeus vannamei. Background technique [0002] Litopenaeus vannamei, also known as white Pacific shrimp, is produced in Central and South America. The wild population is distributed in the Pacific coast waters from Mexico to Chile. The natural water temperature in this area is above 20 degrees all year round. my country's Litopenaeus vannamei aquaculture industry has experienced explosive growth since 2000, and has become the country with the highest aquaculture output of Litopenaeus vannamei in the world. In recent years, my country's annual aquaculture output has exceeded 1 million tons. [0003] With the expansion of the breeding scale, the demand for high-quality seedlings is becoming more and more urgent. my country's self-selected varieties have achieved good results, but the current varieties stil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59
CPCA01K61/59Y02A40/81
Inventor 黄皓
Owner 渤海水产育种(海南)有限公司
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