The invention relates to a detection primer, a detection kit, a detection method and application of a chicken Columbia feather genotype, and belongs to the technical field of animal genetic breeding.According to the invention, the insertion of 63bp base sequences from the 33rd site to the 95th site in the upstream region sequence of the RAI14 gene is found to be closely related to the Columbia feather character; therefore, a PCR (Polymerase Chain Reaction) genotyping method aiming at the insertion is established by utilizing the characteristics of the upstream region of the RAI14 gene, so that the Columbia feather chicken gene can be typed. The method for detecting the chicken Columbia feather genotype is simple to operate, rapid in detection and accurate in result, is beneficial to moreeffectively and rapidly selecting the Columbia feather character in breeding practice, accelerates the breeding progress, saves the breeding cost, and provides a rapid molecular breeding method for breeding the Columbia feather chicken character.