62results about How to "Speed ​​up breeding progress" patented technology

The invention discloses single nucleotide polymorphism of SCD genes in dairy goat and a detection method thereof. The single nucleotide polymorphism of the genes comprises G / A single nucleotide polymorphism which is the 5001st in the SCD genes in the dairy goat. The detection method comprises the following steps: taking the DNA of the whole genome of the dairy goat to be detected containing the SCD genes as a template and a primer pair P as a primer to carry out PCR amplification on the SCD genes in the dairy goat; after using restriction enzyme NdeI to digest the PCR amplified product, carrying out agarose gel electrophoresis on amplified fragments after restriction enzyme; and identifying the 5001bp base polymorphism of the SCD genes in the dairy goat according to the electrophoresis results. The method screens and detects the molecular genetic markers closely related to the milk production traits of the dairy goat on the DNA level to be used for assisted selection and molecular breeding of the dairy goat and speed up find breeding of the dairy goat.

The invention discloses a method for breeding kidding traits by utilizing 3-gene pyramiding effect. The method comprises the following steps: amplifying a 3' untranslated region of a stem cell factor (SCF) gene, an exon 7 of a III type transmembrane tyrosine kinase receptor (KIT) gene and an intron 1 of a kiss (KISS1) gene respectively by using three pairs of primers and by taking goat genome DNA as a template; judging the size of each amplification product by using agarose gel electrophoresis at the concentration of 1.5%; screening the site mutation of the amplification products of the three pairs of primers by a DNA sequencing technology; performing genetic typing and gene frequency analysis on the single nucleotide polymorphisms (SNPs) of three sites of the SCF gene, the KIT gene and the KISS1 gene by using polyacrylamide gel electrophoresis at the concentration of 10% and agarose gel electrophoresis at the concentration of 3.5%; analyzing the relationship between different genotype combinations and kidding quantity; and screening out the optimal genotype combination.

The invention discloses cattle FoxO1 gene mononucleotide polymorphism and a detection method thereof. The gene mononucleotide polymorphism comprises that: with a Qinchuan cattle whole genome DNA comprising the FoxO1 gene as a template, and with a primer pair P as primers, the Qinchuan cattle FoxO1 gene is subjected to PCR amplification; a restriction endonuclease HhaI is used for digesting the PCR amplification product, and the digested segment is subjected to agarose gel electrophoresis; and according to the electrophoresis result, base polymorphism on the 178132 site of the Qinchuan cattle FoxO1 gene is identified. The FoxO1 gene is important for animal muscle fat traits and growth traits. With the method, the molecular genetic marker closely related to Qinchuan cattle growth traits is screened and detected on a DNA level. The method is used in Qinchuan cattle assisted selection and molecular breeding, and assists in accelerating Qinchuan cattle breeding speed.

The invention relates to a method for breeding high-temperature-resistant Paralichthys olivaceus new varieties and belongs to the technical field of agricultural mariculture. In the method, Paralichthys olivaceus half sibs family and sibs family with a clear pedigree are established; multi-generation breeding is performed on the Paralichthys olivaceus family at half lethal temperature; inbreeding possibly occurring in the breeding process and tolerance of the Paralichthys olivaceus at different growth stages of juvenile fish and adult fish to the lethal temperature are taken into full consideration; and screening is repeated for two or more times, so that the high-temperature-resistant Paralichthys olivaceus new varieties can be bred effectively, and the problem that the Paralichthys olivaceus in some provinces in south China are difficult to aestivate.

The invention discloses a multi-gene pyramiding breeding method for thoroughbred milk goats. Genome DNA (deoxyribonucleic acid) of a milk goat continuously giving birth to two or more lambs is used as a template, four pairs of primers respectively expand intron 2 and exon 10 of a prolactin receptor gene and an untranslation region (5'UTR) and exon 1 of luteotropin beta calcmeurin 5', the sizes of expanded products are judged by agarose gel electrophoresis, site mutation of the expanded products of the four pairs of primers are screened by DNA sequencing technology, then polyacrylamide gel electrophoresis is used for performing genetic typing and gene frequency analysis for SNPs (single nucleotide polymorphisms) of four sites of the prolactin receptor gene and the luteotropin beta calcmeurin, the relation of polymorphism of a multiple-birth milk goat individual (F1 generation) and the number of born lamps and the relation of different genetype combinations and the number of the born lamps are analyzed, parental generation (F0 generation) is reviewed, filial generation (F2 generation) is tracked, the relation of the genetype combination of an ewe individual and the number of the born lamps is detected, and contribution of different genetypes in terms of prolific trait formation is analyzed.

The invention discloses single nucleotide polymorphism of the STMN1 gene of a Beijing duck and a detection method thereof. The detection method for the single nucleotide polymorphism of the gene comprises the following steps: with whole genome DNA of a to-be-detected Beijing duck containing the STMN1 gene as a template and the primer pair P as primers, carrying out PCR amplification on the STMN1 gene of the Beijing duck; digesting a PCR amplification product with restriction endonuclease and then carrying out agarose gel electrophoresis on amplified segment after digestion; and identifying polymorphism of the 48th nucleotide base of exon 4 of the STMN1 gene of the Beijing duck according to results of electrophoresis. According to the invention, molecular genetic markers closely related to economic characters of the Beijing duck are screened and detected at the level of DNA, which provides molecular bases for rapid selective breeding of the Beijing duck.

The invention discloses a method for selecting a molecular marker for goat yeaning traits, which comprises the following steps of: taking a goat genome DNA sequence as a template, amplifying KITL gene introns 1 and 6 by using primers P1 and P2 under a PCR condition in the presence of TaqDNA polymerase, buffer environment, Mg2+ and dNTPs respectively, and judging the size of a destination fragment according to an agarose gel electrophoresis result; digesting the PCR amplification product of the primer P1 by using restriction enzyme CviAII, and then detecting the enzyme digested amplification fragment by using polyacrylamide gel electrophoresis, wherein the amplification product of the primer P1 has mutation of two basic groups; detecting the PCR amplification product of the primer P2 by adopting the polyacrylamide gel electrophoresis, wherein the amplification product of the primer P2 has mutation of one basic group; and then performing gene analysis and gene frequency analysis on the amplification products of the primers P1 and P2, and performing association analysis between the amplification products and the yeaning numbers of Guanzhong dairy goats, western Saanen dairy goats and Boer goats, wherein the analysis results show that an SNPs site of the KITL gene, detected by the primers P1 and P2, can be taken as the molecular marker for goat yeaning trait selection.

The invention discloses a method for detecting single nucleotide polymorphism of a sheep KITLG gene and application of the method. The method comprises the following steps: carrying out PCR amplification on fragments of the sheep KITLG gene in a manner of taking a whole-genome DNA of sheep to be detected as a template and taking a primer pair P as primers; digesting a PCR amplification product with restriction endonuclease SspI, and then, carrying out agarose gel electrophoresis on the amplified fragments subjected to digestion; identifying the polymorphism of a 47569-th base of the sheep KITLG gene according to electrophoresis results. The mutant site of the base is closely associated with reproduction traits, i.e., litter size of the sheep, so that the method is a method for detecting molecular genetic markers, which are closely related to the reproduction traits of the sheep, from a DNA level, and can be applied to the assisted selection and molecular breeding of the sheep, and thus, the good-variety breeding speed of the sheep is accelerated.

The invention discloses a molecular marking method of using neuroendocrine factor genes to select kidding characters. The method comprises the following steps of: using a goat genomic DNA sequence as a template; amplifying Kisspeptin gene intron 2 by using a primer P1 under the PCR condition in the presence of TaqDNA polymerase, buffering environment, Mg2+, dNTPs, and then judging the size of the target fragment by agarose gel electrophoresis; detecting the PCR application product of the primer P1 by polyacrylamide gel electrophoresis, finding that the amplification SNPs of the primer P1 has one-base mutation and 8bp-base deletion, and then carrying out genotyping and gene frequency analysis on the amplification product of the primer P1, and the correlation analysis with the kidding characters of Guanzhong diary goat and Xinong Saanen dairy goat. Shown by experiments, the SNPs of the Kisspeptin gene intron 2 detected by the primer P1can be used as molecular marker of the selection of kidding characters.

The invention discloses a whole-genome selective breeding method suitable for aquatic animals. Specifically, high-density SNP typing is carried out on individuals of a breeding basic group or a core group; the phenotypic value of the target character is determined; whole-genome association analysis (GWAS) is carried out by utilizing the SNP typing data and the phenotypic data to obtain a significance P value of each SNP marker; the marks are sorted from low to high according to P values; according to different traits, different marker number combinations sorted in the top are selected according to the P values, analysis is performed by adopting methods such as GBLUP, Bayes B and the like, the prediction accuracy of the different marker numbers selected according to the P values is evaluated through cross validation, and finally, the marker combination with the highest prediction accuracy is determined. SNP typing is conducted on the candidate population or the next-generation breedingpopulation through the screened optimal marker combination, breeding value prediction is conducted on the candidate population or the next-generation breeding population through adoption of GBLUP or BayesB or ssGBLUP or other methods, and therefore, the prediction accuracy can be remarkably improved.

The invention relates to a four-strain mating pair-hybridization breeding method for litopenaeus vannamei, and belongs to the field of genetic breeding for aquatic animals. The method specifically comprises the steps of collecting breeding materials, and constructing four specialized strains including a fast-growth strain (strain A), a high survival / high propagation strain (strain B), a high survival / fast growth strain (strain C) and a high propagation strain (strain D); conducting primary hybridization testing between the fast-growth strain (strain A) and the high survival / high propagation strain (strain B) to select an optimal hybridization combination (AB) with high growth speed; meanwhile, conducting primary hybridization testing between the high survival / fast growth strain (strain C)and the high propagation strain (strain D) to select an optimal hybridization combination (CD) with high fertility; conducting secondary hybridization testing between the optimal hybridization combination (AB) with high growth speed and the optimal hybridization combination (CD) with high fertility to screen out an optimal mating combination (ABCD) to serve as commercial young litopenaeus vannamei. The finally-cultivated strain has the advantages of being high in growth speed and survival rate, and shows obvious yield strengths in different breeding modes.

The invention discloses a method for selecting milk production characters to breed a milk goat by utilizing the double gene polymerization effect. Milk goat genome DNA serves as a template, two pairs of primers are used for amplifying the exon 9 of a gene of a PRLR and the intron 1 of a gene of an ELF5 respectively, agarose gel electrophoresis with the concentration of 1.5% is used for judging the sizes of the amplified products, the locus mutations of the amplified products of the two pairs of primers are screened based on the DNA sequencing technique, then, the agarose gel electrophoresis with the concentration of 3.5% is used for carrying out genetic typing and gene frequency analyzing on SNPs of the two locuses of the gene of the PRLR and the gene of the ELF5, the relation between different gene type combinations and the milk production characters is analyzed, and the optimal gene combination is screened out.

The invention discloses a method for detecting single nucleotide polymorphism of a sheep PCNP (PEST-Containing Nuclear Protein) gene by using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and application of the method. The method comprises the following steps: carrying out PCR amplification on a sheep PCNP gene segment by taking a sheep whole genome DNA to be measured as a template and taking a primer pair P as a primer; after a PCR amplification product is digested by a restriction enzyme SPhI, carrying out agarose gel electrophoresis on the digested amplified segment; authenticating the single nucleotide polymorphism of the 5019th site of the sheep PCNP gene according to an electrophoresis result. As a base mutation site is closely associated with the kidding number of sheep reproduction traits, the method is a method for detecting molecular genetic markers closely associated with the sheep reproduction traits at the DNA level, and can be used for auxiliary selection and molecular breeding of sheep and increasing the improved variety breeding speed of the sheep.

The invention discloses a method for fast detecting the single nucleotide polymorphism (SNP) of a cattle krupple-like factor (KLF) 7 gene. The method comprises the following steps of: performing polymerase chain reaction (PCR) amplification on the cattle KLF7 gene by taking a whole genome DNA comprising a KLF7 gene of cattle to be detected as a template and the mixture of primer pairs P1, P2, P3 and the like in a mol ratio as a primer; after digesting a PCR amplification product by using a restriction enzyme TaqI, performing agarose gel electrophoresis on an amplified fragment subjected to enzyme digestion; and according to the result of the agarose gel electrophoresis, identifying the SNP of the 41041st, 42025th and 42075th positions of the cattle KLF7 gene. In the method, the SNPs of the KLF7 gene are subjected to genotyping and gene frequency analysis, and the growth traits of Nanyang cattle in different growth stages are subjected to trait association analysis. The results show that the SNP locus for the KLF7 gene, particularly P2, can serve as a molecular marker selected in the early stage (6 month age and 12 month age) of the growth traits of the cattle.

The invention provides an SNP molecular marker related with pig growth velocity and application thereof. According to the SNP molecular marker disclosed by the invention, a TRPC1 gene is utilized as acandidate gene which can affect the pig growth velocity; a sequencing technique is utilized to screen and identify SNP loci related with the pig growth velocity to obtain 2 SNP loci which can affectthe pig growth velocity and a gene type effect; the 2 SNP loci are respectively arranged (595bp)th and (539bp)th positions of a sequence shown in SEQ ID NO.1; allelotype of the 2 SNP loci is C and T;furthermore, alleles of C and C and T and T of the 2 SNP loci are completely linked on a genome; thus, any SNP locus can be applied to breeding pig breed growth rate characters; furthermore, the SNP loci cannot be limited by ages, genders and breeds of pigs, can be applied to early breeding of the pigs and can quicken a breeding process.

The invention discloses a method for detecting the polymorphism of three base mutations of the gene exon 4 of goat gonadotropin releasing hormone and the gene exon 2 of the growth differentiation factor 9. The method comprises the following steps: using the DNA sequence of goat genome as template, using 2 to amplify the primer P1 and P2 under PCR condition in the presence of Taq DNA polymerase, buffer environment, Mg2+ and dNTPs, using agarose gel electrophoresis to judge the size of the target fragments; using polyacrylamide gel electrophoresis to detect the PCR amplified product of the primer P1 and P2 to find that the two amplification sites have three base mutations, then performing genotyping and gene frequency analysis to the SNPs of the two genes, and performing association analysis with the fecundity traits of Boer goat and Xinong Saanen dairy goat. The result shows that the SNPs sites detected by the primer P1 and P2, of the GnRH gene exon 4 (containing partial intron 3 and 3' non-translational region) and the GDF9 gene exon 2, thus the method can be used for molecular marking of fecundity trait selection of goat.

The invention discloses a method for detecting insertion-deletions polymorphism of sheep FTH-1 (ferritin heavy polypeptide1) genes by utilizing PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and application thereof. The method comprises the following steps: taking to-be-detected sheep whole genome DNA containing FTH-1 genes as a template, performing PCR amplification on the sheep FTH-1 gene segments, and performing detection and genotyping on the PCR product by utilizing the SSCP technology; and identifying the 639th locus insertion-deletions polymorphism of the sheep FTH-1 genes according to electrophoretic results. The method is a method for screening and detecting a molecular genetic marker which is closely related to reproductive performances of sheep on the DNA level so as to be used for assistant selection and molecular breeding of the sheep and acceleration of sheep stock breeding.

The invention discloses a method for detecting single nucleotide polymorphism of a sheep FTH-1 (Ferritin Heavy Polypeptide-1) gene by using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and application of the method. The method comprises the following steps: carrying out PCR amplification on a sheep FTH-1 gene segment by taking a sheep whole genome DNA to be measured as a template and taking a primer pair P as a primer; after a PCR amplification product is digested by a restriction enzyme XspI, carrying out agarose gel electrophoresis on the digested amplified segment; authenticating the single nucleotide polymorphism of the sheep FTH-1 gene according to an electrophoresis result. As the polymorphism of a base mutation site is closely associated with the kidding number of sheep reproduction traits, the polymorphism detection method is a method for detecting molecular genetic markers closely associated with the sheep reproduction traits at the DNA level, and can be used for auxiliary selection and molecular breeding of sheep and increasing the improved variety breeding speed of the sheep.

The invention relates to a duck sebum character living body prediction method and application thereof. The method comprises the following steps: feeding a batch of ducklings in stages, recording the weight and feed intake of each duck in each stage, calculating the residual feed intake and feed conversion rate of each duck after the feeding is completed, obtaining a body size index and a serum biochemical index of each duck, selecting a batch of ducks from the bred ducks as a to-be-detected group, slaughtering the ducks to obtain sebum traits of the ducks in the to-be-detected group, calculating a multiple linear regression equation of the sebum traits of the ducks by using statistical analysis software, and predicting the residual group sebum traits of the raised ducks by using the multiple linear regression equation. According to the method, the sebum traits of the live ducks can be quickly and accurately estimated, the elimination probability of excellent individuals in breeding is greatly reduced, and the breeding efficiency is greatly improved.

The invention belongs to the field of molecular genetics, and provides an SNP molecular marker for CF1 gene of Qinchuan cattle and a detection method of the SNP molecular marker. The whole genome DNAof Qinchuan cattle to be tested containing the CFL1 gene is used as a template, a primer pair P is used as primers, and PCR is carried out to amplify the CFL1 gene; agarose gel electrophoresis is performed on the amplified fragments after enzyme digestion, after the PCR amplified product is digested with a restriction enzyme HinfI; and the base polymorphism at position 2052 of the CFL1 gene of Qinchuan cattle is identified according to the electrophoresis results. Because the mutation site is closely related to the beef traits (body length, chest width, chest depth and weight) of Qinchuan cattle, and the method is a method for screening and detecting the molecular genetic markers closely related to the growth traits of Qinchuan cattle at DNA level, the SNP molecular marker and the method can be used in auxiliary selection and molecular breeding of Qinchuan cattle, so as to accelerate the breeding speed of Qinchuan cattle.

The invention discloses a single nucleotide polymorphic locus of a cattle I-mfa gene and a detection method thereof. The detection method of the single nucleotide polymorphisms locus of cattle I-mfa gene comprises utilizing cattle whole-genome deoxyribonucleic acid (DNA) to be detected containing the I-mfa gene as a template, utilizing a primer pair P as primers, performing polymerase chain reaction (PCR) amplification on the cattle I-mfa gene and performing polyacrylamide gel electrophoresis on amplified framorphism; according to electrophoresis results, identifying the 12284 locus of the cattle I-mfa gene to be nucleotide polymplate of A or G; and identifying the 12331 locus of the cattle I-mfa gene to be nucleotide polymorphism of T or C. The two locus is completely in linkage. The detection method of the single nucleotide polymorphic locus of the cattle I-mfa gene is a method which screens and detects molecular genetic markers closely related to growth traits of cattle from the DNA level, and provides the molecular basis for cattle crossbreeding utilizing the crossbreed advantages.

The invention discloses a screening method of a goat COX II gene segment capable of serving as a molecular marker and application thereof. The screening method comprises the following steps: by using constructed goat sperm pool DNA (deoxyribonucleic acid) as a template, using a designed specific primer P1 set a corresponding program in the presence of Tag DNA polymerase, buffer, Mg<2+> and dNTPs (deoxyribonucleotide triphosphates), and carrying out PCR (polymerase chain reaction) amplification on the COX II gene; determining the size of the target segment according to an agarose gel electrophoretogram; sequencing to obtain sequence and mutant site information, wherein the result indicates that the amplification product has one base mutant; after digesting HindI II the P1 amplification product with restriction endonuclease, detecting the digested amplification segment by agarose gel electrophoresis; and analyzing the genotype and gene frequency of the P1 amplification product as well as the correlation of the sperm activities between the Anhwei white goat and Bohr goat, wherein the result indicates that the SNP (single nucleotide polymorphism) site of the COX II gene detected by the primer P1 can serve as a molecular marker for selecting goat sperm activity property, thereby providing a molecular marker for auxiliary selection of a goat sperm quality property marker.

The invention discloses a method for selecting milk production characters to breed a milk goat by utilizing the double gene polymerization effect. Milk goat genome DNA serves as a template, two pairs of primers are used for amplifying the exon 9 of a gene of a PRLR and the intron 1 of a gene of an ELF5 respectively, agarose gel electrophoresis with the concentration of 1.5% is used for judging the sizes of the amplified products, the locus mutations of the amplified products of the two pairs of primers are screened based on the DNA sequencing technique, then, the agarose gel electrophoresis with the concentration of 3.5% is used for carrying out genetic typing and gene frequency analyzing on SNPs of the two locuses of the gene of the PRLR and the gene of the ELF5, the relation between different gene type combinations and the milk production characters is analyzed, and the optimal gene combination is screened out.

The invention discloses a method for evaluating the quality of eggshell colloidal protection membranes with different colors. The method comprises: measuring the change of an XYZ color space by usinga spectrophotometer before and after an eggshell colloidal protection membrane is stained with a MST colloidal protection membrane blue, converting the XYZ color space into a RGB color space through aformula, finally converting into opacity, and evaluating the quality of the eggshell colloidal protection membrane by calculating the change of the opacity before and after the staining of the eggshell colloidal protection membrane, wherein the higher the change of the opacity before and after the staining of the eggshell colloidal protection membrane, the better the quality of the eggshell colloidal protection membrane, and the smaller the change of the opacity before and after the staining of the eggshell colloidal protection membrane, the worse the quality of the eggshell colloidal protection membrane. According to the present invention, the method has characteristics of simple operation, low cost and short detection time, and can be generalized to various poultry eggs with colloidal protection membranes, such that the variety with the excellent colloidal protection membrane and the variety with the poor colloidal protection membrane quality can be hybridized so as to select the variety with the excellent colloidal protection membrane quality, reduce the bacterial invasion chance on poultry eggs, and prolong the storage and fresh-keeping time of poultry eggs.

The invention provides an SNP molecular marker located on pig chromosome 9 and related to the healthy piglet number and the healthy piglet rate. The site of the SNP molecular marker corresponds to C > T mutation at the 33597148 site on a chromosome 9 of a reference sequence of an international pig genome version 11.1. By optimizing the dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the number and rate of healthy piglets of core group sows are improved, and the improvement progress of related reproductive traits of the sows is accelerated, so that the economic benefit of breeding of boars is effectively improved.

The invention relates to a detection primer, a detection kit, a detection method and application of a chicken Columbia feather genotype, and belongs to the technical field of animal genetic breeding.According to the invention, the insertion of 63bp base sequences from the 33rd site to the 95th site in the upstream region sequence of the RAI14 gene is found to be closely related to the Columbia feather character; therefore, a PCR (Polymerase Chain Reaction) genotyping method aiming at the insertion is established by utilizing the characteristics of the upstream region of the RAI14 gene, so that the Columbia feather chicken gene can be typed. The method for detecting the chicken Columbia feather genotype is simple to operate, rapid in detection and accurate in result, is beneficial to moreeffectively and rapidly selecting the Columbia feather character in breeding practice, accelerates the breeding progress, saves the breeding cost, and provides a rapid molecular breeding method for breeding the Columbia feather chicken character.

The invention discloses a method for detecting insertion-deletions polymorphism of sheep FTH-1 (ferritin heavy polypeptide1) genes by utilizing PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and application thereof. The method comprises the following steps: taking to-be-detected sheep whole genome DNA containing FTH-1 genes as a template, performing PCR amplification on the sheep FTH-1 gene segments, and performing detection and genotyping on the PCR product by utilizing the SSCP technology; and identifying the 639th locus insertion-deletions polymorphism of the sheep FTH-1 genes according to electrophoretic results. The method is a method for screening and detecting a molecular genetic marker which is closely related to reproductive performances of sheep on the DNA level so as to be used for assistant selection and molecular breeding of the sheep and acceleration of sheep stock breeding.

The invention discloses a molecular marking method of using neuroendocrine factor genes to select kidding characters. The method comprises the following steps of: using a goat genomic DNA sequence as a template; amplifying Kisspeptin gene intron 2 by using a primer P1 under the PCR condition in the presence of TaqDNA polymerase, buffering environment, Mg2+, dNTPs, and then judging the size of thetarget fragment by agarose gel electrophoresis; detecting the PCR application product of the primer P1 by polyacrylamide gel electrophoresis, finding that the amplification SNPs of the primer P1 has one-base mutation and 8bp-base deletion, and then carrying out genotyping and gene frequency analysis on the amplification product of the primer P1, and the correlation analysis with the kidding characters of Guanzhong diary goat and Xinong Saanen dairy goat. Shown by experiments, the SNPs of the Kisspeptin gene intron 2 detected by the primer P1can be used as molecular marker of the selection of kidding characters.

The invention discloses a method for detecting single nucleotide polymorphism of a sheep PCNP (PEST-Containing Nuclear Protein) gene by using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and application of the method. The method comprises the following steps: carrying out PCR amplification on a sheep PCNP gene segment by taking a sheep whole genome DNA to be measured as a template and taking a primer pair P as a primer; after a PCR amplification product is digested by a restriction enzyme SPhI, carrying out agarose gel electrophoresis on the digested amplified segment; authenticating the single nucleotide polymorphism of the 5019th site of the sheep PCNP gene according to an electrophoresis result. As a base mutation site is closely associated with the kidding number of sheep reproduction traits, the method is a method for detecting molecular genetic markers closely associated with the sheep reproduction traits at the DNA level, and can be used for auxiliary selection and molecular breeding of sheep and increasing the improved variety breeding speed of the sheep.