Method for detecting single nucleotide polymorphism of sheep FTH-1 (Ferritin Heavy Polypeptide-1) gene by using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and application of method

Abstract

The invention discloses a method for detecting single nucleotide polymorphism of a sheep FTH-1 (Ferritin Heavy Polypeptide-1) gene by using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and application of the method. The method comprises the following steps: carrying out PCR amplification on a sheep FTH-1 gene segment by taking a sheep whole genome DNA to be measured as a template and taking a primer pair P as a primer; after a PCR amplification product is digested by a restriction enzyme XspI, carrying out agarose gel electrophoresis on the digested amplified segment; authenticating the single nucleotide polymorphism of the sheep FTH-1 gene according to an electrophoresis result. As the polymorphism of a base mutation site is closely associated with the kidding number of sheep reproduction traits, the polymorphism detection method is a method for detecting molecular genetic markers closely associated with the sheep reproduction traits at the DNA level, and can be used for auxiliary selection and molecular breeding of sheep and increasing the improved variety breeding speed of the sheep.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to the use of single nucleotide polymorphism (SNP) of sheep functional gene as a molecular genetic marker, in particular to the single nucleotide polymorphism of sheep FTH-1 gene and a detection method thereof. Background technique [0002] In animal breeding, people expect to achieve early selection and improve the accuracy of breeding values ​​by selecting DNA markers that are closely related to growth, reproduction and other traits and are closely linked to quantitative traits, so as to obtain better results in livestock and poultry breeding. Great genetic advances. [0003] Molecular breeding, that is, Molecular Mark-Assist Selection (MAS), is a technology that uses DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. It uses modern molecular The method of combining biology and traditional gene...

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Problems solved by technology

Due to the lack of research in the field of genetic variation of the FTH-1 gene in Chinese sheep, the functional research of this gene locus and the research on the relationship between genetic variation of this gene and reproductive traits have become blank

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