A method for detecting single nucleotide polymorphism of sheep kitlg gene and its application
A single nucleotide polymorphism, sheep technology, applied in the field of molecular genetics, can solve problems such as few reports on sheep research, and achieve the effects of accelerating breeding progress, low cost and simple method
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[0031] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, and the described embodiments are explanations of the present invention, rather than limitations.
[0032]The invention uses the conservative sequence of KITLG gene to design primers to amplify the 468bp fragment of the fifth intron of KITLG gene, and uses the sheep genome as a template to carry out PCR amplification, and the amplified product is sequenced to search for the single nucleotide polymorphism of the amplified fragment ; Conduct trait correlation analysis for the discovered single nucleotide polymorphism, and provide its detection method, making the nucleotide polymorphism of the KITLG gene a molecular genetic marker that can be quickly and conveniently detected, in order to speed up the establishment of a high-quality Provide evidence for breeding sheep populations.
[0033] a. Detection of sheep KITLG gene polymorphism
[0034] 1. ...
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