43results about How to "Accurately estimate breeding values" patented technology

The invention discloses a method for breeding kidding traits by utilizing 3-gene pyramiding effect. The method comprises the following steps: amplifying a 3' untranslated region of a stem cell factor (SCF) gene, an exon 7 of a III type transmembrane tyrosine kinase receptor (KIT) gene and an intron 1 of a kiss (KISS1) gene respectively by using three pairs of primers and by taking goat genome DNA as a template; judging the size of each amplification product by using agarose gel electrophoresis at the concentration of 1.5%; screening the site mutation of the amplification products of the three pairs of primers by a DNA sequencing technology; performing genetic typing and gene frequency analysis on the single nucleotide polymorphisms (SNPs) of three sites of the SCF gene, the KIT gene and the KISS1 gene by using polyacrylamide gel electrophoresis at the concentration of 10% and agarose gel electrophoresis at the concentration of 3.5%; analyzing the relationship between different genotype combinations and kidding quantity; and screening out the optimal genotype combination.

The invention discloses a multi-gene pyramiding breeding method for thoroughbred milk goats. Genome DNA (deoxyribonucleic acid) of a milk goat continuously giving birth to two or more lambs is used as a template, four pairs of primers respectively expand intron 2 and exon 10 of a prolactin receptor gene and an untranslation region (5'UTR) and exon 1 of luteotropin beta calcmeurin 5', the sizes of expanded products are judged by agarose gel electrophoresis, site mutation of the expanded products of the four pairs of primers are screened by DNA sequencing technology, then polyacrylamide gel electrophoresis is used for performing genetic typing and gene frequency analysis for SNPs (single nucleotide polymorphisms) of four sites of the prolactin receptor gene and the luteotropin beta calcmeurin, the relation of polymorphism of a multiple-birth milk goat individual (F1 generation) and the number of born lamps and the relation of different genetype combinations and the number of the born lamps are analyzed, parental generation (F0 generation) is reviewed, filial generation (F2 generation) is tracked, the relation of the genetype combination of an ewe individual and the number of the born lamps is detected, and contribution of different genetypes in terms of prolific trait formation is analyzed.

The invention discloses single nucleotide polymorphism of the STMN1 gene of a Beijing duck and a detection method thereof. The detection method for the single nucleotide polymorphism of the gene comprises the following steps: with whole genome DNA of a to-be-detected Beijing duck containing the STMN1 gene as a template and the primer pair P as primers, carrying out PCR amplification on the STMN1 gene of the Beijing duck; digesting a PCR amplification product with restriction endonuclease and then carrying out agarose gel electrophoresis on amplified segment after digestion; and identifying polymorphism of the 48th nucleotide base of exon 4 of the STMN1 gene of the Beijing duck according to results of electrophoresis. According to the invention, molecular genetic markers closely related to economic characters of the Beijing duck are screened and detected at the level of DNA, which provides molecular bases for rapid selective breeding of the Beijing duck.

The invention discloses a method for detecting single nucleotide polymorphism of a sheep KITLG gene and application of the method. The method comprises the following steps: carrying out PCR amplification on fragments of the sheep KITLG gene in a manner of taking a whole-genome DNA of sheep to be detected as a template and taking a primer pair P as primers; digesting a PCR amplification product with restriction endonuclease SspI, and then, carrying out agarose gel electrophoresis on the amplified fragments subjected to digestion; identifying the polymorphism of a 47569-th base of the sheep KITLG gene according to electrophoresis results. The mutant site of the base is closely associated with reproduction traits, i.e., litter size of the sheep, so that the method is a method for detecting molecular genetic markers, which are closely related to the reproduction traits of the sheep, from a DNA level, and can be applied to the assisted selection and molecular breeding of the sheep, and thus, the good-variety breeding speed of the sheep is accelerated.

The invention discloses a molecular marking method of using neuroendocrine factor genes to select kidding characters. The method comprises the following steps of: using a goat genomic DNA sequence as a template; amplifying Kisspeptin gene intron 2 by using a primer P1 under the PCR condition in the presence of TaqDNA polymerase, buffering environment, Mg2+, dNTPs, and then judging the size of the target fragment by agarose gel electrophoresis; detecting the PCR application product of the primer P1 by polyacrylamide gel electrophoresis, finding that the amplification SNPs of the primer P1 has one-base mutation and 8bp-base deletion, and then carrying out genotyping and gene frequency analysis on the amplification product of the primer P1, and the correlation analysis with the kidding characters of Guanzhong diary goat and Xinong Saanen dairy goat. Shown by experiments, the SNPs of the Kisspeptin gene intron 2 detected by the primer P1can be used as molecular marker of the selection of kidding characters.

The invention discloses single nucleotide polymorphism of GHRHR genes in dairy goat and a detection method thereof. The single nucleotide polymorphism of the genes comprises C/G base polymorphism which is the 231st in the sequence table of the GHRHR gene in the dairy goat shown in SEQ ID NO.1. The detection method comprises the following steps: taking the DNA of the whole genome of the dairy goat to be detected containing the GHRHR genes as a template and a primer pair P as a primer to carry out PCR amplification on the GHRHR genes in the dairy goat; after using restriction enzyme MspI to digest the PCR amplified product, carrying out agarose gel electrophoresis on amplified fragments after restriction enzyme; and identifying the polymorphism of the genes according to the electrophoresis results. The method screens and detects the molecular genetic markers closely related to the milk production traits of the dairy goat on the DNA level to be used for assisted selection and molecular breeding of the dairy goat and speed up find breeding of the dairy goat.

The invention discloses a method for selecting milk production characters to breed a milk goat by utilizing the double gene polymerization effect. Milk goat genome DNA serves as a template, two pairs of primers are used for amplifying the exon 9 of a gene of a PRLR and the intron 1 of a gene of an ELF5 respectively, agarose gel electrophoresis with the concentration of 1.5% is used for judging the sizes of the amplified products, the locus mutations of the amplified products of the two pairs of primers are screened based on the DNA sequencing technique, then, the agarose gel electrophoresis with the concentration of 3.5% is used for carrying out genetic typing and gene frequency analyzing on SNPs of the two locuses of the gene of the PRLR and the gene of the ELF5, the relation between different gene type combinations and the milk production characters is analyzed, and the optimal gene combination is screened out.

The invention discloses a method for fast detecting the single nucleotide polymorphism (SNP) of a cattle krupple-like factor (KLF) 7 gene. The method comprises the following steps of: performing polymerase chain reaction (PCR) amplification on the cattle KLF7 gene by taking a whole genome DNA comprising a KLF7 gene of cattle to be detected as a template and the mixture of primer pairs P1, P2, P3 and the like in a mol ratio as a primer; after digesting a PCR amplification product by using a restriction enzyme TaqI, performing agarose gel electrophoresis on an amplified fragment subjected to enzyme digestion; and according to the result of the agarose gel electrophoresis, identifying the SNP of the 41041st, 42025th and 42075th positions of the cattle KLF7 gene. In the method, the SNPs of the KLF7 gene are subjected to genotyping and gene frequency analysis, and the growth traits of Nanyang cattle in different growth stages are subjected to trait association analysis. The results show that the SNP locus for the KLF7 gene, particularly P2, can serve as a molecular marker selected in the early stage (6 month age and 12 month age) of the growth traits of the cattle.

The invention discloses a method for detecting the polymorphism of three base mutations of the gene exon 4 of goat gonadotropin releasing hormone and the gene exon 2 of the growth differentiation factor 9. The method comprises the following steps: using the DNA sequence of goat genome as template, using 2 to amplify the primer P1 and P2 under PCR condition in the presence of Taq DNA polymerase, buffer environment, Mg2+ and dNTPs, using agarose gel electrophoresis to judge the size of the target fragments; using polyacrylamide gel electrophoresis to detect the PCR amplified product of the primer P1 and P2 to find that the two amplification sites have three base mutations, then performing genotyping and gene frequency analysis to the SNPs of the two genes, and performing association analysis with the fecundity traits of Boer goat and Xinong Saanen dairy goat. The result shows that the SNPs sites detected by the primer P1 and P2, of the GnRH gene exon 4 (containing partial intron 3 and 3' non-translational region) and the GDF9 gene exon 2, thus the method can be used for molecular marking of fecundity trait selection of goat.

The invention discloses a method for detecting insertion-deletions polymorphism of sheep FTH-1 (ferritin heavy polypeptide1) genes by utilizing PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and application thereof. The method comprises the following steps: taking to-be-detected sheep whole genome DNA containing FTH-1 genes as a template, performing PCR amplification on the sheep FTH-1 gene segments, and performing detection and genotyping on the PCR product by utilizing the SSCP technology; and identifying the 639th locus insertion-deletions polymorphism of the sheep FTH-1 genes according to electrophoretic results. The method is a method for screening and detecting a molecular genetic marker which is closely related to reproductive performances of sheep on the DNA level so as to be used for assistant selection and molecular breeding of the sheep and acceleration of sheep stock breeding.

The invention discloses a method for detecting single nucleotide polymorphism of a sheep FTH-1 (Ferritin Heavy Polypeptide-1) gene by using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and application of the method. The method comprises the following steps: carrying out PCR amplification on a sheep FTH-1 gene segment by taking a sheep whole genome DNA to be measured as a template and taking a primer pair P as a primer; after a PCR amplification product is digested by a restriction enzyme XspI, carrying out agarose gel electrophoresis on the digested amplified segment; authenticating the single nucleotide polymorphism of the sheep FTH-1 gene according to an electrophoresis result. As the polymorphism of a base mutation site is closely associated with the kidding number of sheep reproduction traits, the polymorphism detection method is a method for detecting molecular genetic markers closely associated with the sheep reproduction traits at the DNA level, and can be used for auxiliary selection and molecular breeding of sheep and increasing the improved variety breeding speed of the sheep.

The invention belongs to the field of molecular genetics, and provides an SNP molecular marker for CF1 gene of Qinchuan cattle and a detection method of the SNP molecular marker. The whole genome DNAof Qinchuan cattle to be tested containing the CFL1 gene is used as a template, a primer pair P is used as primers, and PCR is carried out to amplify the CFL1 gene; agarose gel electrophoresis is performed on the amplified fragments after enzyme digestion, after the PCR amplified product is digested with a restriction enzyme HinfI; and the base polymorphism at position 2052 of the CFL1 gene of Qinchuan cattle is identified according to the electrophoresis results. Because the mutation site is closely related to the beef traits (body length, chest width, chest depth and weight) of Qinchuan cattle, and the method is a method for screening and detecting the molecular genetic markers closely related to the growth traits of Qinchuan cattle at DNA level, the SNP molecular marker and the method can be used in auxiliary selection and molecular breeding of Qinchuan cattle, so as to accelerate the breeding speed of Qinchuan cattle.

The invention discloses meat-duck OTXR gene single nucleotide polymorphism and a detecting method and application thereof, and belongs to the field of molecular genetics detecting. The detecting process of the gene single nucleotide polymorphism includes the steps that to-be-detected meat-duct whole genome DNA containing OTXR genes serves as a template, a primer pair P serves as a primer, and the meat-duck OTXR genes are subjected to PCR amplification; after the PCR amplification product is digested through restriction enzymes PstI, amplified fragments obtained after digestion are subjected to agarose gel electrophoresis; the polymorphism of basic groups of the 946th nucleotide of second exons of the meat-duck OTXR genes is identified according to the electrophoresis result. According to the meat-duck OTXR gene single nucleotide polymorphism and the detecting method and application thereof, molecular genetic markers closely related to economic characters of meat ducks are screened and detected in the DNA level, and molecular bases are provided for rapid breeding of meat ducks.

The invention aims to provide a method for evaluating the individual breeding value of a turbot random population, so as to calculate an individual parentage coefficient under the condition of non-existence of physical pedigree, and further carry out genetic parameter evaluation. Pedigree recording is not needed in the method, but in traditional breeding, physical marking needs to be performed on a breeding population, and pedigree is recorded to evaluate an inter-individual genetic relationship, so that the workload is high. According to the method, the inter-individual genetic relationship can be directly evaluated by utilizing molecules, so as to eliminate the step of pedigree recording. Moreover, the inter-individual genetic relationship can be evaluated more accurately according to the method.

The invention discloses a screening method of a goat COX II gene segment capable of serving as a molecular marker and application thereof. The screening method comprises the following steps: by using constructed goat sperm pool DNA (deoxyribonucleic acid) as a template, using a designed specific primer P1 set a corresponding program in the presence of Tag DNA polymerase, buffer, Mg<2+> and dNTPs (deoxyribonucleotide triphosphates), and carrying out PCR (polymerase chain reaction) amplification on the COX II gene; determining the size of the target segment according to an agarose gel electrophoretogram; sequencing to obtain sequence and mutant site information, wherein the result indicates that the amplification product has one base mutant; after digesting HindI II the P1 amplification product with restriction endonuclease, detecting the digested amplification segment by agarose gel electrophoresis; and analyzing the genotype and gene frequency of the P1 amplification product as well as the correlation of the sperm activities between the Anhwei white goat and Bohr goat, wherein the result indicates that the SNP (single nucleotide polymorphism) site of the COX II gene detected by the primer P1 can serve as a molecular marker for selecting goat sperm activity property, thereby providing a molecular marker for auxiliary selection of a goat sperm quality property marker.

The invention discloses a single nucleotide polymorphism of an ox TAS1R2 gene and a detecting method thereof. The detecting method of the single nucleotide polymorphism of the gene comprises the following steps of: with an ox total genome DNA to be detected, which comprises a TAS1R2 gene, as a template and with a primer pair P as a primer, carrying out PCR (Polymerase Chain Reaction) amplification on the ox TAS1R2 gene; after denaturing the product, carrying out polyacrylamide gel electrophoresis on a denatured amplification fragment; and identifying the 288bp base polymorphism of the ox TAS1R2 gene according to an electrophoresis result. The method is used for screening and detecting molecular genetic markers which are closely relevant to ox meat producing characters at a DNA level to beused for the assisted selection and molecular breeding of oxen and improving the breeding speed of fine breeds of the oxen.