43results about How to "Accurately estimate breeding values" patented technology

The invention discloses a molecular marking method selecting goat growth traits using nucleophosmin gene. According to the method, a goat gene group DNA sequence is used as a template, under the condition of existence of Taq DNA polymerase, buffer environment, Mg <2+>, and dNTPs, four pairs of primer are used for amplifying a Nucleophosmin (NPM) gene 5'UTR region and exon 1 under the condition of PCR condition, and the size of a target segment of an amplified product is determined according to agarose gel electrophoresis; an amplified site of primer P3 is found lacking of 18bp basic group through detection of PCR amplification products of primers P1-P4 by use of polyacrylamide gel electrophoresis, then genotyping and gene frequency analysis are performed on the amplification product of the primer P3, and associated analysis on the amplification production of the primer P3 and growth traits of a milk goat and a Boer goat in the central Shaanxi plain is performed. Results indicate that the SNPs site of the Nucleophosmin gene exon 1 detected by the primer P3 can be used as a molecular mark for goat growth trait selection.

The invention discloses a method for selecting milk production characters to breed a milk goat by utilizing the double gene polymerization effect. Milk goat genome DNA serves as a template, two pairs of primers are used for amplifying the exon 9 of a gene of a PRLR and the intron 1 of a gene of an ELF5 respectively, agarose gel electrophoresis with the concentration of 1.5% is used for judging the sizes of the amplified products, the locus mutations of the amplified products of the two pairs of primers are screened based on the DNA sequencing technique, then, the agarose gel electrophoresis with the concentration of 3.5% is used for carrying out genetic typing and gene frequency analyzing on SNPs of the two locuses of the gene of the PRLR and the gene of the ELF5, the relation between different gene type combinations and the milk production characters is analyzed, and the optimal gene combination is screened out.

The invention discloses cattle FoxO1 gene mononucleotide polymorphism and a detection method thereof. The gene mononucleotide polymorphism comprises that: with a Qinchuan cattle whole genome DNA comprising the FoxO1 gene as a template, and with a primer pair P as primers, the Qinchuan cattle FoxO1 gene is subjected to PCR amplification; a restriction endonuclease HhaI is used for digesting the PCR amplification product, and the digested segment is subjected to agarose gel electrophoresis; and according to the electrophoresis result, base polymorphism on the 178132 site of the Qinchuan cattle FoxO1 gene is identified. The FoxO1 gene is important for animal muscle fat traits and growth traits. With the method, the molecular genetic marker closely related to Qinchuan cattle growth traits is screened and detected on a DNA level. The method is used in Qinchuan cattle assisted selection and molecular breeding, and assists in accelerating Qinchuan cattle breeding speed.