8 results about "Crossbreed" patented technology

The application discloses a SNP marker (chr1: 170526265, located on the upstream of a SETDB2 gene) related to a twelve-week-old weight trait of a chicken and an application thereof. By determining and recording the twelve-week-old weight of a crossbreed chicken of a crossbreed population of the chicken, 1092 chickens are sequenced by using a resequencing technology, and further whole genome correlation analysis is carried out, so that a SNP (chr1: 170526265) site affecting the twelve-week-old weight is obtained. The SNP frequencies of the SNP site in low-weight chicken species and high-weight chicken species in resequencing are counted, and it is found that the SNP site has significant differences in the low-weight chicken species and the high-weight chicken species. In the high-weight chicken, G is a dominant allele, and in the low-weight chicken, A is a dominant allele. In the population with low weight, by selecting the individuals with the G allele of the site, the weight of the breeding population can be improved.

The utility model discloses a limit type mutton sheep hybridization breeding pen, and particularly relates to the technical field of mutton sheep breeding, the limit type mutton sheep hybridization breeding pen comprises breeding pen bottom frames, two fixing side frames and a supporting frame, the two fixing side frames are both fixed on the upper surface of the breeding pen bottom frames, the supporting frame is fixed on one side of one breeding pen bottom frame, and the supporting frame is fixed on the other side of the breeding pen bottom frame. A limiting pushing assembly is arranged in the supporting frame. The limiting and pushing assembly comprises a linkage screw rod, a transmission motor, a sliding sleeve block, a linkage strip and a linkage block. The limiting and pushing assembly is adopted, a piece of hybridized mutton sheep is placed between the fixing side frames, the piece of hybridized mutton sheep can thoroughly enter the position between the two fixing side frames, a second piece of hybridized mutton sheep is placed between the two fixing side frames, and the second piece of hybridized mutton sheep is rapidly limited to enter the position between the two fixing side frames. Mutton sheep with two ends hybridized can be rapidly limited for hybridization breeding and can be rapidly driven to a designated position, and the hybridization breeding speed is higher.

The application discloses a molecular marker and specific primers and an identification method for identifying Takifugu obscurus, Takifugu poecilonotus and hybrid Arlea japonica. The application can realize multiple test results through one-time PCR, and simultaneously complete the rapid identification of the three species of Takifugu obscurus, Takifugu poecilonotus and hybrid Arlea japonica. The application provides a fast and accurate identification method for Arlea japonica variety identification, solves the problems of difficult naked-eye differentiation and low operation accuracy of morphological feature differentiation, and realizes the characteristics of easy result judgment, high conservation, high accuracy and the like. The application provides technical support for variety identification and parent selection of the special economic fish, provides a train of thought for further carrying out genetic scientific research of hybrid Arlea japonica, and is favorable for promoting the vigorous development of the hybrid Arlea japonica breeding industry.

The present invention discloses a method for constructing and using an animal model of dementia with Lewy bodies, and belongs to the technical field of animal model construction. The construction method comprises: obtaining a phosphatase and tensin homologous gene-induced kinase 1 gene knockout experimental animal A using gene editing technology, and crossbreeding with an Alzheimer's disease model experimental animal B simulating β-amyloid protein (Aβ) pathology, and screening offspring containing both Alzheimer's disease-related mutations and pink1 gene knockout. The model constructed by the present invention is to deposit β-amyloid protein on the basis of pink1 gene knockout, induce α-synuclein protein aggregation using β-amyloid plaques (Aβ), widely produce Lewy bodies in the cortex and hippocampus, simulate the occurrence and development process of DLB, and provide a reliable animal model for disease mechanism research and therapeutic drug screening and development.

The present invention proposes a method for breeding a high-performance Wenchang chicken complementary line. The method comprises: establishing a complementary line breeding population of four Wenchang chicken lines, namely, the specialized Wenchang chicken lines LA, LB, CU3, and CK, with the specialized Wenchang chicken line LA serving as the maternal parent, the specialized Wenchang chicken line LB serving as the maternal parent, the specialized Wenchang chicken line CU3 serving as the paternal parent, and the specialized Wenchang chicken line CK serving as the paternal parent; selecting seeds based on comprehensive indicators to control seed retention rates; and selecting the chicken breed obtained by crossbreeding the terminal maternal and terminal paternal lines as the target complementary line commercial broiler chicken. The Longquan Wenchang chicken complementary line commercial broiler chicken obtained by the present invention not only enhances the high-quality flavor and appearance of the local Wenchang chicken breed, but also significantly improves its production performance.

The present invention provides a method for cultivating hybrid spiny eels, which method comprises: S1. Selection of parent fish; S2. Inducing spawning and fertilization; S3. Culturing fry; and S4. Culturing commercial fish. This cultivation method uses Sinobdella sinensis and Macrognathus aculeatus for hybridization. It can either use male Sinobdella sinensis as the male parent and female Macrognathus aculeatus as the female parent for hybridization and breeding, or use male Macrognathus aculeatus as the male parent and female Sinobdella sinensis as the female parent for hybridization and breeding, and can obtain a population of offspring with the hybrid advantages of the parents in a short time, improving the problems of poor cold tolerance and population degradation of Macrognathus aculeatus itself.

The invention relates to the technical field of biological breeding, in particular to a primer group and a kit for detecting an SNP (Single Nucleotide Polymorphism) marker related to a cattle carcass weight character and application of the primer group and the kit. The primer group comprises an upstream primer F as shown in SEQ ID NO.2 and a downstream primer R as shown in SEQ ID NO.3, the SNP marker detected by the primer group and related to the carcass weight character of the cattle is located at the 59342623bp position on the 20th chromosome of the cattle, an A / G mutation site exists at the 59342623bp position, so that the SNP marker has three genotypes of AA, AG and GG, and the carcass weight of individuals with the genotypes of AA and AG is remarkably higher than that of individuals with the genotypes of GG. The method is used for screening carcass heavy individuals with excellent snowflake beef production aiming at the hybrid population of the He cattle and the Angus cattle, and can be used for early marker-assisted selective breeding to accelerate the breeding process.

The present invention relates to the technical field of animal genetic engineering, and particularly relates to a humanized animal model of the CRBN gene, a construction system, a construction method and an application. C57BL / 6J strain mice are used, and C57BL / 6J is abbreviated as B6; specifically, B6.Fas<supgt;lpr / lpr< / supgt> strain, B6.Ncf1<supgt;m1j / m1j< / supgt> strain, B6.Crbn<supgt;tm1.1Ble< / supgt> strain, B6.Stat1<supgt;fl / fl< / supgt> strain, B6.Cd68<supgt;Cre / Cre< / supgt> strain, and B6.Lyz2<supgt;Akaluc< / supgt> strain mice are used to establish breeding cages and perform cross-breeding to finally obtain an animal model of four types of mice; the present invention combines the polymorphism of the Ncf1 gene and the macrophage tracing method of Lyz2 gene-targeted spontaneous fluorescence labeling. By mating a Cre-labeled mouse transcribed from the promoter position of the membrane protein Cd68-encoding gene with a Stat1-Floxp mouse, a conditional gene knockout CKO mouse is established, and a mouse model with multiple controls and a wide application range is established, which is helpful for the research of immune diseases and the development of drugs for the treatment of immune diseases.