The application discloses a
molecular marker and
specific primers and an identification method for identifying
Takifugu obscurus, Takifugu poecilonotus and
hybrid Arlea
japonica. The application can realize multiple test results through one-time PCR, and simultaneously complete the
rapid identification of the three species of
Takifugu obscurus, Takifugu poecilonotus and
hybrid Arlea
japonica. The application provides a fast and accurate identification method for Arlea
japonica variety identification, solves the problems of difficult naked-eye differentiation and low operation accuracy of morphological feature differentiation, and realizes the characteristics of easy result judgment, high conservation, high accuracy and the like. The application provides
technical support for variety identification and parent selection of the special economic fish, provides a
train of thought for further carrying out genetic scientific research of
hybrid Arlea japonica, and is favorable for promoting the vigorous development of the hybrid Arlea japonica breeding industry.