109 results about "Crossbreed" patented technology

The invention provides a method of crossbreed seed producing which performs rhizocarpous regeneration by use of the cotton sterile male line and restorer line, and the steps are that: a. first year, in the region where the cotton is able to live through the winter, the cotton male sterile line and the restorer line are respectively planted, the restorer line is used as the male parent to crossbreed with the sterile line to produce the F1 crossbreed cotton seeds so as to being used for the large-area production; b. after gaining the seeds, the sterile line and the restorer line plant live through the winter in natural environment; c. next year, the sterile line and the restorer line plant through the winter are pruned and trained, subsequently, the restorer line is used as the male parent to crossbreed with the sterile line during the flowering period, again the F1 crossbreed cotton seeds are produced so as to being used for the large-area production; d. the two steps of b and c are repeated for 1 to 3 times. The invention promises one year planting and continuously gaining the crossbreed seeds for many years, the invention provides labor and time saving for crossbreed seed production with low cost; moreover, the advantages of the crossbreed can be kept for a long time and the planting output and the quality of the crossbreed cotton can be assured, which is convenient for the commercialized application and has extremely important significance in the popularization of the utilization technology of the cotton crossbreed advantages.

The invention relates to a cultivation method of the triploid salvia, the steps of the invention are that: not completely ripe good salvia seeds are collected to put into the 1/2MS culture medium with colchicine for mutagenesis cultivation; then the seeds are transferred into a normal 1/2MS culture medium for cultivation, the root tip of the seedling is fetched to perform the ploidy identification of the chromosome after the preceding process of the chromosome, and the seedling with a chromosome quantity of 2n=4x=32 is the tetraploid salvia; the tetraploid salvia is taken as a female parent and the diploid salvia is taken as a male parent to perform the crossbreed and the obtaining seed is a triploid salvia plant. The nutrient organs such as the root, stem and leaf and so on of the triploid salvia plant obviously become bigger and present remarkable crossbreed advantages, and the root medicinal material output of the triploid salvia is increased by over 30 percent. Moreover, the triploid salvia has flowers, but no seeds, thus decreasing the nutrition consumption and the crossbreed advantages of the triploid salvia can be kept over 10 years through the asexual reproduction, which enhances the disease resistance and stress resistance with strong applicability, thus being applicable to planting in every part of the country. Besides, the crossbreed advantages produced by the novel variety triploid production are able to improve the output of the salvia medicinal material planting in a large scale.

The sprayer of this invention is a crossbreed that combines the benefits of a pressurized air sprayer with the convenience a common bottle-top trigger sprayer. However, activating the trigger now has a reverse action causing the related pump to ingest air and the pump's traditional flow direction is now reversed. Air is passed into a reinforced reservoir bottle, near the top, similar to a pressurized air sprayer. Multiple hand-trigger-pulls are used to pre-pressurize air into the reservoir's chamber. A second conduit starting within and near the bottom of the reservoir utilizes air pressure to transfer product within the reservoir to a dispensing-valve. A means is provided to operate the dispensing-valve, which is able to dispense product continuously in-between compression strokes.

The invention provides a five-in-one variety breeding method. The five-in-one variety breeding method comprises the following steps: firstly, taking a hybridized combination A*B or a population variety C as a base material, planting the base material in a strong stress environment at high density and large group, continuously carrying out selfing and test mating by using an IPT ((Isopentenyl-Transferases) method, planting the test mating progenies in regions with remarkably different ecological environments and selecting single plants D of which the test mating progenies meet certain standard; secondly, carrying out DH (Doubled Haploid) breeding by taking the D as the material to obtain more than 1000 haploid seeds, doubling the haploids to obtain more than 100 DH pure lines, carry outing target character selection, genetic background restoration selection and homozygosity identification by using an MAS (Marker Assisted Selection) molecule marker assisted breeding technology and selecting a DH pure line F according with a breeding target; thirdly, taking F as parent and assembling the F with the traditional common pure line cultivar to form a hybridized combination, planting the hybridized combination at more than ten test points in different ecological regions and selecting hybridized combination with strong adaptability, high quality and high yield as a hybrid variety G for breeding. According to the five-in-one variety breeding method provided by the invention, the breeding efficiency is increased.

The invention discloses a high-efficiency polymerization method for apple cross breeding. The cross breeding is the most important and most effective way of the breeding of the apples, but the evaluation and the screening of crossbreed seedlings and cross breeding fruits take a lot of work and labors, steps of the cross breeding method are complicated, and the improper technology of each step can cause the failure of the breeding. The breeding period of the apples is long and needs at least 20 years from the cross breeding to the new species selecting and breeding. The method optimizes the key steps of the cross breeding of the apples and overcomes the defects of the conventional breeding method. The method adopts the technical schemes of anther collection; artificial cross breeding; cross breeding management; cross breeding seed collection and pregermination; cross breeding young seedling management; early selection of cross breeding seedlings; primary selection and reselection; and excellent series area demonstration. Compared with the conventional method, the high-efficiency polymerization method specifically and effectively selects indexes, can shorten the breeding period of the apples by 3-4 years and save the breeding cost of the apples by 15-20 percent, and the method system achieves the international advanced level.

The invention provides a method of the aseptic seeding and culturing of the cymbidium aseptic seed, comprising the steps of aseptic seeding, aseptic seedling culturing and seedling hardening, which is characterized in that after the aseptic seeding of the seed, a low temperature and darkness pre-culture step is added. The invention aims at enabling the cymbidium aseptic seed to complete the after ripening process and to promote germination by using the low temperature and dark condition and simulating the proterozonic environment of the cymbidium aseptic seed. Compared with the prior art, the invention improves the average bourgeon rate of the aseptic seeding of the cymbidium aseptic seed by over 10 times and has the advantages of simpleness and practicality, short seed culturing period and convenience for a large-scale production and so on, and the invention lays a firm basis for the novel variety selection of the cymbidium aseptic crossbreed.

The invention discloses a breeding method of a yellow bud marker photosensitive male sterile line. The breeding method provided by the invention comprises the following step: performing autocopuation on the yellow bud marker photosensitive male sterile line under the condition of being within 11 hours and 12 hours and 20 minutes so as to get seeds of the yellow bud marker photosensitive male sterile line. The yellow bud marker photosensitive male sterile line is the sterile line, is also a maintainer line of the sterile line, and further has the characteristics of a wide range of restoration sources, free assembly, simple seed production procedure and low seed production cost. The yellow bud marker photosensitive male sterile line comprises a yellow bud marker, hybrid seeds can be effectively removed by screening markers during the seedling stage and photosensitive sterile strains can be further determined. The yellow bud marker photosensitive male sterile line can provide seed resources for breeding of the new photosensitive sterile lines, greatly reduce the cost of artificial emasculation, assemble a large number of hybrid combinations, breed the hybrid seeds and accelerate the breeding process.

The invention relates to an industrial production method of Cymbidium germchit based on sexual hybridization. The method provided by the invention comprises the following steps of: firstly identifying chromosome number and ploidy of Cymbidium parents; selecting pairing hybridized combinations according to the ploidy; carrying out a sexual hybridization pollination of the Cymbidium; managing the pollinated Cymbidium; carrying out a nonsymbiotic germination culture on Cymbidium seeds; inducing and differentiating protocorms (PLBs) of the Cymbidium seeds; differentiating bigger seedlings of the Cymbidium; transplanting the Cymbidium seedlings into bottles; and managing the cultivation of the Cymbidium transplanted seedlings. On the basis of indentifying the chromosomes of the Cymbidium parents, the method provided by the invention selectively pairs the hybridized combinations and carries out the sexual hybridization pollination, so that the aims of improving varieties and breeding new varieties are achieved; then a lot of Cymbidium germchit can be industrially produced through a tissue culture method.

The invention relates to a method for producing a cotton crossbreed, which uses a pure line variety of an HB red flower upland cotton as a male parent or a female parent to perform hybridization with a normal white flower parent material so as to generate a crossbreed and belongs to the field of agricultural biotechnology. The red flower upland cotton has stable characteristic heredity, and the expression of the red flower upland cotton is not influenced by climate, cultivation conditions and the like; through field planting observations of F1, F2 or BC1F1, the purity and the truth of an F1 generation crossbreed intuitively, quickly and easily according to flower color separation, and the identification result is reliable, quick and accurate. A false crossbreed can be pulled out during the bud stage in production to reduce the economic loss caused by problems of crossbreed purity, and the method provides an effective technical support for the protection of a cotton new variety and the seed quality detection control for cotton seed enterprises and majority of cotton farmers, and has important application value and social meaning.

A crossbreed method for different geographical species parti-colour abalone, which relates to a genetic improved method for parti-colour abalone, especially relates to a crossbreed method using different geographical species parti-colour abalone which comprises, putting the abalone in 16-18Deg C. sea water and elevating temperature to make the parent abalone accelerate. The bait comprises gracilaria 100, Ulva lactuca 30-60, pinnatifida 20-50, spirulina 1-15. Injecting 5-hydroxytryptamine to the head or abdominal feet of the parti-colour abalone, and making the lay eggs and discharge spermium synchronous with a different geographical parti-colour abalone. Making artificial insemination and getting filial generation. and guaranteeing the survival rate during the line haul, making a two species parent parti-colour abalones mature in a same time by using reasonable bait breeding; Using ejecting 5-hydroxytryptamine to make the gamete discharge in a same time and overcome the physiological difference of gamete discharging and sexual maturity because of geographic isolation of different geographical species, and making the gonadal mature and gamete discharge at the same time.

The invention relates to a pelteobagrus vachelli and pseudobagrus ussuriensis cross breeding method, which is characterized by comprising the steps of parent selection and breeding, outbreeding, cross bred fingerling culture and the like. By the pelteobagrus vachelli and pseudobagrus ussuriensis cross breeding method, a crossbreed of pelteobagrus vachelli and pseudobagrus ussuriensis, which has rapid growth, strong disease resistance and excellent meat quality can be obtained; and the method has significance for improving specific yield, breeding benefit, and serving production practice.

The invention provides a production method of high-efficient and high-quality indigenous chicken and the utilization thereof. The production method includes the steps: selecting an indigenous strain as the patriline, selecting a strain with the same feather color with the patriline as the matriline, and hybridizing a cock of the patriline and a hen of the matriline to obtain a crossbreed of F1 generation, i.e. a high-quality indigenous variety commercial generation chicken. The invention also provides a food containing the ingredient of the chicken obtained by the production method of the high-efficient and high-quality indigenous chicken. The commercial generation chicken (F1 generation) produced by the invention retains the macroscopic features of the indigenous variety chicken and the characteristic of fresh meat quality, improves the production performances in various aspects, embodies the economic value of the indigenous variety and better satisfies the market requirement.

The invention relates to the aquatic product heredity and breeding field, in particular to a construction method of a portunus trituberculatus miers disease-resistant variety. The method comprises the following concrete steps: information of mitochondrial control zone sequence of breeding materials serves as a molecular marker, group with rich heredity mutation serves as base group of group breeding according to the molecular marker, and groups which are genetically independent serve as the supplementary materials of family breeding; first, group breeding is carried out on the base group to obtain group, family breeding is carried out on the obtained group and the supplementary materials of family breeding, then, interfamily hybridization combination is carried out on groups with stable disease-resistant character obtained from family breeding, and the family with stable disease-resistant character is the disease-resistant variety; wherein breeding materials in each step are individuals which survive with half lethal concentration after being soaked and infected by source of infection. The invention shortens the breeding time while making the most use of species genetic resources simultaneously, combines the adaptability of local crabs, and effectively avoids the inbreeding depression of small groups, thus facilitating scientifically cultivation of the portunus trituberculatus miers disease-resistant variety suitable for local aquaculture.

The invention provides an insect pollination seed producing method for the cotton nuclear sterile male lines in the rhizocarpous condition, the steps of the invention are that: in the region where the cotton is able to live through the winter, the cotton worm-resistant nuclear sterile male lines are planted as a female parent and the restorer line are planted as a male parent; during the bud period of the first year, the viable plants in the female parent are pulled out, and in the flowering and boll setting period, the production of the crossbreed is performed by use of the artificial assistant pollination or insect pollination; in winter, the female parent is rhizocarpous, the male parent is sleeved and planted into the rhizocarpous cotton of the second year of which the female plant is 100 percent sterile plant; in the flowering period, the cotton crossbreed seeds by means of insects pollination are adopted. In the third year and later, the insect pollination is performed in the flowering period by utilizing the female parent and the male parent of the rhizocarpous cotton to produce the crossbreed seeds. The advantage of the crossbreed can be kept for a long time and the planting output and the quality can be assured, the method promises planting one year and gaining crossbreed seeds continuously for many years; and by utilizing the insect pollination, a large amount of labor forces are saved and the seed production cost is low, which is convenient for the commercialized application and has extremely important significance in the popularization of the utilization technology of the cotton crossbreed advantages.

The invention discloses an RNA quantitative detection method using the cutting single strand nucleic acid characteristic of S1 enzyme. The method mainly comprises the following steps: first, fixing a capture probe with proper concentration to a enzyme label plate precoated by the streptavidin for further use; then ultrasonically lysising the cell and releasing RNA from the cell, filling in signal probe and hybridizing with targeting RNA; after hybridizing, filling in S1 enzyme to cut the single strand part in the single RNA,DNA and RNA/signal probe crossbreed, obtaining the complete complementary targeting RNA/signal probe crossbreed; hybridizing between the capture probe and the signal probe after the rest crossbreed is denaturated, finally amplifying the signal by horseradish peroxidase. The invention has simple operation and strong specificity. The RNA quantitative detection can be finished under conventional experimental conditions.

The invention discloses a molecular marker of a main active gene BLS1 of paddy rice resisting bacterial streak and an application of the molecular marker. A bacterial streak resistant backcross inbred line obtained from deriving an ordinary wild paddy rice resistant source DP3 is crossed with a bacterial streak-infected nonglutinous rice variety 93-11 to obtain an offspring F1 hybrid and then the hybrid is self-fertilized to obtain F2 generation, genetic linkage analysis is performed for a gene type and corresponding resistant phenotype of each family of F2, and the bacterial streak-resistant main active gene BLS1 sourced from the ordinary wild paddy rice DP3 is detected. Molecular markers capable of being used for breeding such as RM19382, RM19391, RM19400, RM19402 and RM510 are obtained. The molecular markers can effectively detect whether the bacterial streak-resistant ordinary wild paddy rice DP3 and erivative varieties (lines) thereof contain a main active gene site, thereby greatly improving the selection efficiency of the bacterial streak resistant paddy rice, and obtaining the bacterial streak resistant paddy rice variety containing the BLS1 gene.

A hybridization method for wheat features that the wheat 'Mi7606(1)' as female parent and the branched wheat '33' as male parent are used for hydridizing and after choosing several times a new variety of hybridized wheat is cultured on the basis of the second generation. Its advantages are high quality, and high stress resistance and reproduction speed.

The invention belongs to the technical field of poultry breeding, and particularly relates to a breeding method for a new strain of high-yield green-eggshell laying hens with relatively high egg laying performance. The method comprises the following steps: hybridizing Langshan chickens serving as a male parent and the green-eggshell laying hens serving as a female parent to generate a generation F1, selecting black-feather hens of the generation F1 for continuous hybridization with the male parent in the step a to generate a generation F2, selecting hens of the generation F2 for hybridization with the green-eggshell laying hens to obtain a generation F3, performing crossing and expanding propagation, and breeding the new strain of the high-yield green-eggshell laying hens after breeding of 3 generations. The new strain of high-yield green-eggshell laying hens are relatively high in egg yield; compared with the original female parent, the high-yield green-eggshell laying hens have the egg production week shortened by 2 weeks or longer, and the annual egg output is increased by 40 pieces or more.

The invention discloses a method and application method for obtaining a novel germ plasm of brassica A genome vegetable. In the method, free microspore culture is carried out on crossbreeds among vegetables of Chinese cabbage subspecies*pakchoi cabbage subspecies or variety in pakchoi cabbage subspecies*variety of the brassica A genome to obtain a regeneration plant and further to create the novel germ plasm which is used for breeding. The method mainly comprises the following steps of: hybridizing the vegetables of Chinese cabbage subspecies*pakchoi cabbage subspecies or variety in pakchoi cabbage subspecies*variety; inducing free microspore culture embryoids; germinating the embryoids to obtain the regeneration plant; carrying out ploidy identification on the regeneration plant obtainedby microspore culture; reserving seeds of double haploids and polyploids; planting an offspring of the regeneration plant to carry out morphological identification and nutritive index identification;and preparing, hybridizing and combining. The method has the advantages of short cycle and high efficiency, provides a material for efficiently breeding Chinese cabbage vegetables and has broad application prospect.

The invention relates to the technical field of crossbreeding and in particular relates to a crossbreeding method of industrial hemp. The crossbreeding method comprises the following steps: seeding female parents DMG231, and seeding male parents DMG240; carrying out interlaced planting on the female parents DMG231 and the male parents DMG240; hybridizing the female parents DMG231 and the male parents DMG240 to obtain F1-generation seeds; and planting the F1-generation seeds to obtain F1-generation plants. Hologynic tall cannabidiol and industrial hemp crossbreeds cultivated by using the crossbreeding method are high in CBD content, are all female and are suitable for the characteristics of planting in southern areas in China.

The invention discloses a convergent cross breeding method for multi-resistance high-quality orange heart baby cabbage for spring and autumn. In order to solve problems, the method comprises the following steps of: selectively breeding a high-stalk encircling resistance source 322; selectively breeding an encircling orange heart material 02-693; selectively breeding a premature orange heart self-bred line 08P238; hybridizing a TuMV resistance source B75 and Fushan wrapped cabbage head, and then performing multi-generation inbred to obtain a property modified encircling resistance source 95; selectively breeding a bolting-resistant material 538; selectively breeding an extremely early orange heart strain 304; selectively breeding an orange heart baby cabbage self-bred line X08-219; performing coordinating test on the premature orange heart self-bred line 08P238 and the orange heart baby cabbage self-bred line X08-219; transforming the premature orange heart self-bred line 08P238 into a sterile line to obtain an orange heart male sterile line 08P238A; and hybridizing the orange heart baby cabbage self-bred line X08-219 and the orange heart male sterile line 08P238A to obtain an F1 crossbreed. The orange heart baby cabbage selectively bred by the method for spring and autumn is extremely early, has high virus disease resistance and orange color, and is suitable to be cultivated in spring and autumn.

According to the present invention, provided is a production method of a crossbreed of Takifugu obscurus and Takifugu rubripes, which comprises the following steps: extracting ova and semen from female Takifugu obscurus and male Takifugu rubripes respectively; performing artificial fertilization to obtain fertilized ova; and incubating the fertilized ova and breeding the same. According to the present invention, a new crossbreed of Takifugu obscurus and Takifugu rubripes produced by the production method has an advantage of rapid growth rate, thereby allowing the promotion of existing puffer culture industry declining due to low production rate and low economical efficiency.

The invention belongs to the filed of crop inheritance breeding, relating to a method for breading crossbreed with corn inbred lines. The Kuan-chung Plain in the northwest china has good geography advantage; however, the current per mu yield of corn crops is not extremely higher than the other areas in the whole country and stability is bad. The invention provides a method for breading crossbreed with corn inbred lines, in the year of 2003, inbred lines m3255 is taken as female parent in Hainan, foreign introduction lines 1*9801 is taken as male parent, according to the ratio of the female parent and the male parent being 3:1 or 4:1, cross-combined crossbreed with density being 4000-4500 seed/per mu is seeded; the leaf sheath of seedling has the color of dark violet, the cotyledon has a shape of elliptic type, the leaves have the color of dark green, the leaf edges have the color of green, and the leaves are upwards straightened; the crop is high to 245-250 cm, the seed is high to 85-90 cm, the crop shape is compact and the root system is developed; the seed has the shape of long cylinder, the spike stalk has the color of white, the ear length is 14-16 cm, the head progeny row is 12-14; and each row has 30-33 grains, the seed is strong and full. The method has the advantages of high yielding, stable yielding, strong disease resistance, wide applicability, strong stress resistance and good agriculture shape, and is fit for sowing corn in the spring and the winter at the northwest china and north China.

The invention discloses a method for breeding the male sterility restoring line of yellow sprout pepper cytoplasm and belongs to the technical field of plant breeding. The method includes the specific steps that mixed pollination is conducted on the male sterility restoring line of the yellow sprout pepper cytoplasm and selfing line pepper male parent pollen, harvested hybrid seeds are sowed, the fertile plant line and fertile plants are screened many times, strict selfing is conducted, and the male sterility restoring line of the yellow sprout pepper cytoplasm with the restoring rate being 100% is obtained finally; compared with an existing breeding technology of the pepper male sterility restoring line, the method has the advantages that the method is simple in breeding procedure, convenient to implement and controllable in target trait and procedure of breeding; the breeding efficiency is improved greatly, the breeding restoring line type and number are increased greatly, and a new purpose is developed for advantage utilization of pepper crossbreed, leaf color marking sterility restoring line breeding and the like.

The invention discloses a rapid breeding method of potatoes. Screening is conducted by methods of screening in Shandong and Inner Mongolia as well as screening spring, summer and autumn, varieties suitable for a central plain two-season crop area and a northern one-season crop area simultaneously can be selected, and potato new varieties only suitable for the central plain two-season crop area toplant or only suitable for the northern one-season crop area to plant also can be selected, so compared with the traditional breeding method, the rapid breeding method of the potatoes has the following advantages: the prepared hybridized combination is completely utilized and waste of hybridized materials is avoided; and through generation adding planting of two places, the operation from preparation of hybridized combination in the first year to variety registration totally needs 11 years, the breeding year limit is shortened and the breeding flow is accelerated, so that the pace of pushing the new varieties to the market is accelerated.

The invention discloses a method for performing variety identification and seed purity check of a celery cabbage by using an SCAR marker. The method uses a genome DNA of a new species 'gold crown No.2' and a parent thereof of an orange celery cabbage as a template, establishes an RAPD specific finger print of a crossbreed 'gold crown No.2' crossbreed and the parent thereof through an RAPD analysis, successfully obtains a female parent specificity SCAR marker and a crossbreed specificity SCAR marker, and can perform the variety identification and the seed purity check of the 'gold crown No.2' crossbreed according to a variety finger print and specificity markers by using the two SCAR markers. The identification results of the identifications of 134 random groups of the 'gold crown No.2' accord with those of field identifications.