Method for cultivating triploid of red sage root

A cultivation method and triploid technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, etc., can solve problems such as lack of repatriation and improvement, achieve strong heterosis and reduce nutrient consumption , the effect of improving biological yield

Inactive Publication Date: 2008-02-20
NANKAI UNIV
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Problems solved by technology

However, there is a lot of research work to be carried out urgently in the discovery, innovation and directional cultivation of new varieties of salvia miltiorrhiz...

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  • Method for cultivating triploid of red sage root
  • Method for cultivating triploid of red sage root

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Embodiment

[0024] Figure 1 shows the chromosome analysis and identification of diploid (a), triploid (b) and tetraploid (c) of Salvia miltiorrhiza; Figure 2 shows diploid (a), triploid (b) and tetraploid of Salvia miltiorrhiza (c) Plant appearance; Figure 3 is a comparison of the diploid (a), triploid (b) and tetraploid (c) flowers of Salvia miltiorrhiza.

[0025] The white-flowered salvia miltiorrhiza collected from the Laiwu area of ​​Shandong Province took root every two years and was inserted in the experimental field in early March. When it bloomed, it was bagged and self-crossed to prevent cross-pollination. When the seeds are close to maturity (not blackened, still green), about 50 immature seeds are taken, first sterilized with 75% alcohol for 10 minutes, then sterilized with 0.1% mercury liter for 10 minutes, then rinsed with sterile water 5 times, inoculated In 1 / 2 MS medium containing 0.02% colchicine, culture at room temperature 25° C. for 96 hours. Then transfer the Salvi...

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Abstract

The invention relates to a cultivation method of the triploid salvia, the steps of the invention are that: not completely ripe good salvia seeds are collected to put into the 1/2MS culture medium with colchicine for mutagenesis cultivation; then the seeds are transferred into a normal 1/2MS culture medium for cultivation, the root tip of the seedling is fetched to perform the ploidy identification of the chromosome after the preceding process of the chromosome, and the seedling with a chromosome quantity of 2n=4x=32 is the tetraploid salvia; the tetraploid salvia is taken as a female parent and the diploid salvia is taken as a male parent to perform the crossbreed and the obtaining seed is a triploid salvia plant. The nutrient organs such as the root, stem and leaf and so on of the triploid salvia plant obviously become bigger and present remarkable crossbreed advantages, and the root medicinal material output of the triploid salvia is increased by over 30 percent. Moreover, the triploid salvia has flowers, but no seeds, thus decreasing the nutrition consumption and the crossbreed advantages of the triploid salvia can be kept over 10 years through the asexual reproduction, which enhances the disease resistance and stress resistance with strong applicability, thus being applicable to planting in every part of the country. Besides, the crossbreed advantages produced by the novel variety triploid production are able to improve the output of the salvia medicinal material planting in a large scale.

Description

technical field [0001] The invention relates to a method for cultivating new varieties of salvia miltiorrhiza, in particular to a method for cultivating triploid salvia miltiorrhiza. Through the heterosis produced by the new triploid variety, the method for greatly improving the yield of the cultivated salvia miltiorrhiza medicinal material. Background technique [0002] The root of Danshen (Salviamiltiorrhza Bunge) is a traditional traditional Chinese medicine for promoting blood circulation and removing blood stasis, which is widely used clinically to treat coronary heart disease, angina pectoris, ischemic stroke and other diseases. At present, there are more than 100 kinds of Danshen and its compound preparations produced in my country. However, the production of Salvia miltiorrhiza in my country has been in short supply since the mid-1970s. With the development of cities and population, the wild resources of Salvia miltiorrhiza continue to decrease, and cultivated Salv...

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Application Information

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IPC IPC(8): A01H1/02A01H1/08A01H5/00C12Q1/68
Inventor 陈力李秀兰陈成彬宋文芹
Owner NANKAI UNIV
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