83 results about "Ploidy" patented technology

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention relates to the technical field of bioinformatics and molecular cytogenetics, in particular to a method for counting centromeres of chromosomes based on a Nuf2 antibody and application. The method comprises the following steps: performing chromosome centromere counting on a Nuf2 antibody and a centromere specific protein Nuf2 through an immunofluorescence reaction, so that the number of chromosome centromeres can be directly observed, and the number of chromosomes can be efficiently and quickly determined. Besides, due to the fact that the specific protein Nuf2 of the centromere is highly conservative in eukaryotes, the invention further provides application of the method to identification of the plant ploidy, the method is easy and convenient to operate and low in cost, the ploidy identification efficiency is remarkably improved, and a universal technical platform is provided for genetic breeding.

The invention discloses a method for cultivating triploid kelp sporophytes and identifying ploidy of induced protonema, and belongs to the field of kelp breeding. The method is based on a capillary glass needle separation technology for inducing filamentous somatic cells by kelp and a molecular biological technology for specific marking of sex of kelp gametophytes. The sex specific marker of the kelp gametophyte is used for carrying out PCR (Polymerase Chain Reaction) amplification and 1.5% agarose gel electrophoresis detection on the separated induction protonema single-cell cloning line, so that the genetic sex of the kelp induction protonema can be accurately identified; and the ploidy of the induced protonema of a part of varieties (lines) is directly determined from the molecular level. The method for synchronously detecting the genetic sex and ploidy of the induced protonema breaks through the bottlenecks of chromosome counting and flow cytometry operation, can be completed only through one-time PCR amplification, and is simple, convenient, rapid, short in time consumption, capable of completing detection of a plurality of samples at the same time and low in cost.

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

Provided herein are methods of determining a treatment for a subject having a neoplastic disease, said method comprising assaying a sample obtained from the subject for (a) SAC activity, (b) ploidy, (c) WGD, (d) APC / C activity, or (e) a combination thereof. In exemplary embodiments, the treatment determined for the subject comprises, consists essentially of, or consists of a KIF18A inhibitor, when the sample is positive for (a) increased SAC activity, (b) high ploidy, (c) WGD, (d) low APC / C activity, (d) or a combination thereof.

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

The invention belongs to the technical field of plant detection, and particularly relates to a strawberry root tip chromosome slide preparation method. According to the method, strawberry root tips are used as test materials, and clear chromosome division phases of strawberries can be obtained through enzymatic hydrolysate and hypotonic treatment of the strawberry root tips. According to the strawberry root tip chromosome slide preparation method provided by the invention, the root taking frequency is reduced, the observation time is saved, the strawberry resource ploidy can be accurately identified, and the efficiency of obtaining the clear chromosome metaphase of the strawberry is remarkably improved.

The utility model provides a device capable of quantitatively adding DNA (Deoxyribose Nucleic Acid) ploidy staining fluid, which relates to the technical field of fluid injection, and comprises a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder and a fluid taking cylinder, and is characterized in that the fluid taking cylinder can be used for temporarily storing the staining fluid under the action of air pressure, and one end of the fluid taking cylinder is provided with an outlet; the electromagnetic liquid extraction component is in a rod shape, one end of the electromagnetic liquid extraction component is arranged in the liquid extraction cylinder to serve as a piston, the other end of the electromagnetic liquid extraction component extends out of the liquid extraction cylinder to be pushed, and the magnetic force of the electromagnetic liquid extraction component can be changed. During use, the permanent magnet ring is arranged in the liquid extraction cylinder, the movable electromagnetic liquid extraction component is arranged in the liquid extraction cylinder, the magnetic force of the electromagnetic liquid extraction component can be changed, and the repulsive force of the permanent magnet ring to the electromagnetic liquid extraction component is changed by changing the magnetic force of the electromagnetic liquid extraction component; the end part of the electromagnetic liquid extraction part is pushed to a specified scale, and the staining fluid is quantitatively extracted, so that the effect of quantitatively adding the staining fluid is realized.

The invention discloses a system for endometriosis diagnosis and a computer storage medium. Aiming at major adjustment of endometriosis diagnosis standards, biomarkers and ploidy information of circulating endometrial cells in easily available samples such as peripheral blood of a patient are detected, and comprehensive risk assessment is performed by utilizing an optimization algorithm model, so that the diagnosis accuracy of endometriosis is improved. The invention provides an objective and quantifiable noninvasive diagnostic tool adapted to the adjusted standard, and the method solves the problem of missed diagnosis caused by false negative in the existing surgical diagnostic method, and has important clinical application value, social benefit and market prospect for improving the overall diagnostic level of endometriosis.

This invention provides a chromosome copy number counting method based on the relative fluorescence intensity between chromosome monosoms. This method leverages the built-in genetic markers of different chromosomes in multiplex STR typing systems. By introducing a control sample with known ploidy, and considering that the relative fluorescence intensity between two chromosome monosoms is a constant value, the ploidy of chromosomes with unknown ploidy in the test sample is calculated. This method yields accurate results that can be cross-validated with ploidy determinations based on genetic marker typing, eliminating the need for further screening of other polymorphic genetic markers or additional experimental studies to determine the ploidy of the chromosome. Furthermore, it can serve as a basis for developing corresponding software for multiplex STR typing systems. Developing corresponding analytical software based on this calculation scheme can significantly reduce manual intervention in data analysis, improve the accuracy of analytical results, and significantly reduce experimental workload.

The present invention provides a hexaploid Salvia miltiorrhiza and a cultivation method, which relate to the technical field of hexaploid Salvia miltiorrhiza cultivation, including chromosome manipulation technology. The hexaploid Salvia miltiorrhiza and the cultivation method include the following steps: Sp1, collecting diploid Salvia miltiorrhiza germplasm resources, collecting 6 materials from Yunnan, Sichuan, Shaanxi, Shandong, Hebei, Tianjin and other places, and establishing a Salvia miltiorrhiza germplasm resource material planting nursery; Sp2, performing morphological observation, trait analysis, chromosome ploidy identification and genome analysis on the Salvia miltiorrhiza germplasm resources, the results of which show that the somatic cells of white Salvia miltiorrhiza and purple Salvia miltiorrhiza are all diploid, that is, 2n=2x=16; Sp3, based on the identification results, selecting diploid white Salvia miltiorrhiza and artificially inducing tetraploid Salvia miltiorrhiza using a chemical mutagen. Taking the mutagenized white Salvia miltiorrhiza seedlings for chromosome ploidy identification, screening homozygous tetraploid white Salvia miltiorrhiza, and then performing triploid hybridization of the tetraploid white Salvia miltiorrhiza and the diploid purple Salvia miltiorrhiza.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention provides a novel screening platform and aneuploidal pluripotent stem cells and compositions containing for use in the identification of genes / pathways affected by different ploidy states or chromosomal stability and therapeutic agents that selectively act on cells of different ploidy states or chromosomal stability. In particular the present invention provides novel methods for inducing aneuploidy and / or chromosomal instability in human pluripotent stem cells (hPSCs) or non-human pluripotent stem cells (nhpPSCs). The present application also relates to the use of such methods and aneuploid hPSCs or nhpPSCs obtained thereby for identifying genes which are affected by different ploidy states and / or chromosomal stability and for identifying therapeutic agents that selectively or preferentially act on cells of different ploidy states and / or which affect chromosomal stability.

The invention discloses an embryo development quality evaluation method and device, and belongs to the field of embryo in-vitro culture, the embryo before implantation is subjected to three-dimensional imaging to form a three-dimensional blastocyst structure chart, fluorescence imaging is carried out to form a redox specific heat chart, the three-dimensional blastocyst structure chart and the redox specific heat chart are subjected to co-localization superposition, and the embryo development quality is evaluated. Constructing a three-dimensional blastocyst model based on map combination; according to the three-dimensional blastocyst model, the spatial form, chromosome ploidy and metabolic state of the early blastocyst are comprehensively evaluated, and non-intrusive, accurate and efficient blastocyst ectodevelopment quality evaluation and transplantation screening are achieved.

The application discloses a set of detection wheat relative species universal markers and application thereof, and belongs to the technical field of plant genetic engineering. The application takes different species of triticeae as research objects, selects specific primers designed on the basis of the length polymorphism information of different introns of the conserved genes of triticeae identified by using the existing Chinese spring wheat reference genome and annotation information and a bioinformatics method, and finally develops the polymorphic DNA molecular markers capable of distinguishing different ploidy materials of triticeae by using PCR technology and agarose gel electrophoresis technology. The molecular markers have high specificity, stability and universality, and provide a new direction for identification of exogenous chromosome fragments of wheat, molecular marker assisted breeding of exogenous genes and screening of germplasm resources.

The invention relates to the field of plant biotechnology breeding, and discloses a strawberry anther culture regeneration plant optimization method based on multistage screening, which comprises the following steps: constructing a regeneration first generation regeneration plant population through in vitro induction culture; detecting the ploidy of a single plant by using flow cytometry, and removing plants with abnormal ploidy according to a ploidy detection result; calculating a genetic similarity coefficient between the ploidy-stable plant and the parent by using a simple repetitive sequence marker, and removing a genetic variation plant to retain a superior line group; planting the superior line group in a field, and then measuring agronomic, physiological and fruit quality indexes; integrating ploidy, genetic coefficient and multi-dimensional character indexes to establish a grading evaluation model, and dividing the plants into different grades to establish a superior line. By constructing a cell, molecule and phenotype three-level linkage screening system, the screening efficiency and accuracy of strawberry anther culture regeneration plants are improved, and objective quantification of optimal line establishment is realized.

The present invention relates to the technical fields of bioinformatics and molecular cytogenetics, and particularly relates to a method and application for chromosome centromere counting based on Nuf2 antibody. The present invention comprises the following steps: performing chromosome centromere counting on the Nuf2 antibody and the centromere-specific protein Nuf2 through an immunofluorescence reaction, so as to directly observe the number of chromosome centromeres, and thus efficiently and rapidly determine the number of chromosomes. In addition, in view of the high conservation of the centromere-specific protein Nuf2 in eukaryotes, the present invention also provides the application of this method in identifying the ploidy of plants. The operation is simple, the cost is low, the efficiency of ploidy identification is significantly improved, and a general technical platform is provided for genetic breeding.

The invention discloses a method for virus-free rapid propagation by utilizing strawberry anther culture, and relates to the technical field of strawberry anther culture, and the method is characterized by comprising the following steps: S1, selection and disinfection of explants, S2, culture stage, S3, ploidy identification, S4, virus detection, and S5, domestication and transplanting of virus-free seedlings. According to the strawberry anther in-vitro culture method, a large number of virus-free plants are successfully obtained through a strawberry anther in-vitro culture technology, the strawberry detoxification efficiency and effect are remarkably improved, and technical support is provided for healthy development of the strawberry industry.

The invention relates to the technical field of plant breeding, discloses a high-quality and high-yield alfalfa directional breeding method and application thereof, and aims to solve the problem that western alfalfa breeding lacks an integrated molecular marker screening system which is combined with a time sequence stress phenotype and can be simultaneously associated with high-protein quality, drought resistance and saline-alkaline characteristics. The method comprises the following steps: constructing association and verification double groups, and performing stress memory activation, ploidy and genetic purity verification and quality preliminary screening; simulating three-stage time sequence stress from a seedling stage to an early overwintering stage, and measuring four-dimensional phenotypic indexes of yield, quality, stress response and habitat adaptation; gWAS analysis is carried out, a double-character co-association marker is mined, and molecular expression, genetic complementation and multi-environment verification are carried out; finally, new germplasm is created through phenotype primary screening, genotype verification, ecological adaptation three-level screening and field trials, the breeding efficiency is improved, the bred variety can stably adapt to the western composite habitat, the high-yield and high-protein characteristics and the soil improvement capacity are both considered, and the field ineffective workload is greatly reduced.

The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.

The present application relates to the field of aquatic organism breeding technology, and particularly relates to a method for preparing rainbow trout tetraploid fry. The method provided by the present application comprises the following steps: obtaining fertilized eggs by dry fertilization of naturally matured rainbow trout parents, inducing the rainbow trout fertilized eggs to obtain tetraploid rainbow trout after 6.2-6.4 hours, and the duration of the induction is 10-18 minutes; after the tetraploid rainbow trout fry is obtained by induction, the chromosome ploidy of the fish is determined by using techniques such as chromosome karyotype analysis and flow cytometry DNA content determination, and the tetraploid rainbow trout fry is identified and screened for cultivation. The present application adopts a drug induction method, and controls the starting time and duration of the induction to inhibit the first mitosis time of the fertilized eggs, thereby improving the induction rate and survival rate of the tetraploid rainbow trout, and the induction rate of the tetraploid rainbow trout can reach 25-30%, and the survival rate of the fry can reach more than 90%.

The application discloses a method for creating a DH line of eggplant by using anther culture and application thereof, and relates to the technical field of plant breeding. The application establishes a morphological index of a flower bud based on a calyx lobe separation state, replaces a time-consuming microscope examination procedure, and optimizes a combination of heat treatment and low-temperature pretreatment, so that the embryoid induction rate is significantly improved. Finally, through strict ploidy identification and self-pollination purification, a DH line with consistent traits and stable heredity can be obtained, an efficient pure line source is provided for eggplant hybrid breeding, and efficient conversion from a heterozygous genotype plant to a homozygous DH line is realized.

The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.

The present invention relates to the field of biotechnology, and in particular to a MT molecular marker related to a potato tuber solanine content regulating gene and its application, and to determining the potato flesh solanine inhibitory factor by amplification results of a marker detection primer set. StERF9 The ploidy of potatoes can be determined by the type and expression level of the promoter, and the level of solanine in the mature potato flesh can be predicted. This greatly shortens the time required to measure solanine in potato flesh after the potato growth cycle is completed. It is a new breeding method that saves time and is highly accurate.

This disclosure provides a novel method and system using a “spatial genome aligner” that parses true chromatin signals from noise by aligning signals to a DNA polymer model. This spatial genome aligner can efficiently reconstruct chromosome architectures from DNA-fluorescence in situ hybridization (DNA-FISH) data across multiple scales and determine chromosome ploidies de novo in interphase cells.