11 results about "Ploidy" patented technology

The present application relates to the field of aquatic organism breeding technology, and particularly relates to a method for preparing rainbow trout tetraploid fry. The method provided by the present application comprises the following steps: obtaining fertilized eggs by dry fertilization of naturally matured rainbow trout parents, inducing the rainbow trout fertilized eggs to obtain tetraploid rainbow trout after 6.2-6.4 hours, and the duration of the induction is 10-18 minutes; after the tetraploid rainbow trout fry is obtained by induction, the chromosome ploidy of the fish is determined by using techniques such as chromosome karyotype analysis and flow cytometry DNA content determination, and the tetraploid rainbow trout fry is identified and screened for cultivation. The present application adopts a drug induction method, and controls the starting time and duration of the induction to inhibit the first mitosis time of the fertilized eggs, thereby improving the induction rate and survival rate of the tetraploid rainbow trout, and the induction rate of the tetraploid rainbow trout can reach 25-30%, and the survival rate of the fry can reach more than 90%.

The application discloses a method for creating a DH line of eggplant by using anther culture and application thereof, and relates to the technical field of plant breeding. The application establishes a morphological index of a flower bud based on a calyx lobe separation state, replaces a time-consuming microscope examination procedure, and optimizes a combination of heat treatment and low-temperature pretreatment, so that the embryoid induction rate is significantly improved. Finally, through strict ploidy identification and self-pollination purification, a DH line with consistent traits and stable heredity can be obtained, an efficient pure line source is provided for eggplant hybrid breeding, and efficient conversion from a heterozygous genotype plant to a homozygous DH line is realized.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

This invention discloses a method for creating maize androgenetic haploid inducible lines and its applications. This invention belongs to the field of biotechnology. The substances regulating the expression of protein-coding genes or regulating the activity or content of said proteins can be applied in any of the following ways: U1) application in regulating the induction ability of plant androgenetic haploids; U2) application in preparing products that regulate the induction ability of plant androgenetic haploids; U3) application in cultivating plants with altered androgenetic haploid induction ability; U4) application in preparing products that cultivate plants with altered androgenetic haploid induction ability; U5) application in plant breeding. This invention can identify haploids through the compact, leafless plant phenotype, and further confirm their ploidy using flow cytometry, greatly reducing screening costs and time, and has promising applications.

The present application relates to the assisted evaluation method of the second biopsy in the pre-implantation genetic testing. The blastocyst first biopsy process collects and cryopreserves the corresponding biopsy droplet; for the blastocyst with CNV detection failure or suspected abnormal CNV detection result in the first biopsy cell, the first biopsy droplet is thawed to complete genome amplification, CNV library construction, high-throughput sequencing and CNV analysis, and the second biopsy suggestion is given according to the detection result. If the biopsy droplet detects the abnormal aneuploidy or chimera clinically interpreted, it is suggested that the corresponding blastocyst has a high probability of chromosomal abnormality, and the second biopsy is not recommended; if the biopsy droplet does not detect the abnormal aneuploidy or chimera clinically interpreted, it is suggested that the corresponding blastocyst has a high probability of chromosomal normality, and the second biopsy is recommended. The present application proves that the first biopsy droplet contains detectable genetic material, and the consistency of the chromosomal ploidy detection result and the biopsy cell is more than 80%, which can assist the second biopsy decision and improve the decision efficiency and scientificity of PGT detection.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The present application firstly obtains the diploid Fujian oyster common, shell gold and shell black strains with fast growth and pure shell color through population breeding. Secondly, the tetraploid Fujian oyster common, shell gold and shell black strains with high and stable tetraploid rate and stable shell color inheritance are obtained through ploidy breeding. Then, the tetraploid populations of long oyster 'Haida 1', 'Haida 2' and 'Haida 3' are obtained by using ploidy breeding technology with the pure shell color, shell type and large size of the individuals as parents. Finally, the fast-growing and high-temperature-resistant hybrid triploid oyster new strains with different shell colors are obtained through interspecific hybridization. The hybrid triploid oyster new strains obtained in the present application maintain the fast growth and strong stress resistance of long oyster, and have the excellent high-temperature-resistant traits of Fujian oyster. In addition, the shell color of the gold and black hybrid triploid oyster new strains is pure and the color is beautiful. The hybrid triploid oyster new strains obtained in the present application expand the germplasm resources of the shell color strains of the cultured oyster, provide important germplasm resources for the high-quality development and transformation and upgrading of the oyster industry to the high-end consumer market, and have a broad industrial application prospect.

The application provides a tumor single sample whole genome copy number calculation method and device. The calculation method comprises the following steps: S1) obtaining the copy number signal and allele frequency of the tumor single sample; S2) performing first segmentation analysis on the copy number signal and allele frequency to obtain a first segmentation set; S3) identifying a low heterozygosity density region, and screening a corrected heterozygous site set based on the distribution of the allele frequency of the tumor single sample; S4) performing second segmentation analysis on the corrected heterozygous site set and the copy number signal to obtain a second segmentation set; and S5) calculating the purity and ploidy of the tumor single sample based on the second segmentation set, and calculating the whole genome copy number in the tumor single sample. The application can solve the problem that there is no allele copy number analysis method suitable for the whole genome and having high accuracy without a control sample in the prior art, and is suitable for the field of bioinformatics.

A method for establishing a high-efficiency tissue culture system for Atractylodes lancea includes: determining the optimal conditions for adventitious bud proliferation of Atractylodes lancea var. guananus and Atractylodes lancea var. benjamin; screening the optimal culture media for rooting and seedling vigor of the two Atractylodes lancea varieties; determining the appropriate number of days for hardening off tissue culture seedlings; obtaining the complete growth cycle of tissue culture seedlings after transplanting; verifying the ploidy of tissue culture seedlings and their corresponding seedlings; measuring the content of secondary metabolites (atractylodes lancea); and establishing a highly efficient and stable rapid tissue culture propagation method for Atractylodes lancea var. guananus and Atractylodes lancea var. benjamin). The advantages of this invention are: achieving efficient rapid tissue culture propagation while simultaneously ensuring stable ploidy of tissue culture seedlings, strong field adaptability, and stable quality, thus accelerating the protection of high-quality Atractylodes lancea resources and the breeding of new varieties.

This invention discloses a method for inducing haploids in Camellia oleifera through radiation pollen pollination, belonging to the field of plant tissue culture. The method includes the following steps: irradiating Camellia oleifera pollen; determining the viability and germination rate of the irradiated pollen; performing hybridization pollination using the irradiated pollen; selecting the pollinated embryos and inoculating them into a culture medium for embryo rescue; and screening the plants obtained from the embryo rescue for ploidy identification to identify haploids. This invention creates a new approach for cultivating haploids in Camellia oleifera, applicable to both the Camellia oleifera and Camellia brevicornuate groups, and can be applied to the breeding of Camellia oleifera varieties, accelerating the Camellia oleifera breeding process.