48 results about "Ploidy" patented technology

The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention discloses a method for cultivating triploid kelp sporophytes and identifying ploidy of induced protonema, and belongs to the field of kelp breeding. The method is based on a capillary glass needle separation technology for inducing filamentous somatic cells by kelp and a molecular biological technology for specific marking of sex of kelp gametophytes. The sex specific marker of the kelp gametophyte is used for carrying out PCR (Polymerase Chain Reaction) amplification and 1.5% agarose gel electrophoresis detection on the separated induction protonema single-cell cloning line, so that the genetic sex of the kelp induction protonema can be accurately identified; and the ploidy of the induced protonema of a part of varieties (lines) is directly determined from the molecular level. The method for synchronously detecting the genetic sex and ploidy of the induced protonema breaks through the bottlenecks of chromosome counting and flow cytometry operation, can be completed only through one-time PCR amplification, and is simple, convenient, rapid, short in time consumption, capable of completing detection of a plurality of samples at the same time and low in cost.

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

The utility model provides a device capable of quantitatively adding DNA (Deoxyribose Nucleic Acid) ploidy staining fluid, which relates to the technical field of fluid injection, and comprises a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder, a fluid taking cylinder and a fluid taking cylinder, and is characterized in that the fluid taking cylinder can be used for temporarily storing the staining fluid under the action of air pressure, and one end of the fluid taking cylinder is provided with an outlet; the electromagnetic liquid extraction component is in a rod shape, one end of the electromagnetic liquid extraction component is arranged in the liquid extraction cylinder to serve as a piston, the other end of the electromagnetic liquid extraction component extends out of the liquid extraction cylinder to be pushed, and the magnetic force of the electromagnetic liquid extraction component can be changed. During use, the permanent magnet ring is arranged in the liquid extraction cylinder, the movable electromagnetic liquid extraction component is arranged in the liquid extraction cylinder, the magnetic force of the electromagnetic liquid extraction component can be changed, and the repulsive force of the permanent magnet ring to the electromagnetic liquid extraction component is changed by changing the magnetic force of the electromagnetic liquid extraction component; the end part of the electromagnetic liquid extraction part is pushed to a specified scale, and the staining fluid is quantitatively extracted, so that the effect of quantitatively adding the staining fluid is realized.

The invention discloses a system for endometriosis diagnosis and a computer storage medium. Aiming at major adjustment of endometriosis diagnosis standards, biomarkers and ploidy information of circulating endometrial cells in easily available samples such as peripheral blood of a patient are detected, and comprehensive risk assessment is performed by utilizing an optimization algorithm model, so that the diagnosis accuracy of endometriosis is improved. The invention provides an objective and quantifiable noninvasive diagnostic tool adapted to the adjusted standard, and the method solves the problem of missed diagnosis caused by false negative in the existing surgical diagnostic method, and has important clinical application value, social benefit and market prospect for improving the overall diagnostic level of endometriosis.

This invention provides a chromosome copy number counting method based on the relative fluorescence intensity between chromosome monosoms. This method leverages the built-in genetic markers of different chromosomes in multiplex STR typing systems. By introducing a control sample with known ploidy, and considering that the relative fluorescence intensity between two chromosome monosoms is a constant value, the ploidy of chromosomes with unknown ploidy in the test sample is calculated. This method yields accurate results that can be cross-validated with ploidy determinations based on genetic marker typing, eliminating the need for further screening of other polymorphic genetic markers or additional experimental studies to determine the ploidy of the chromosome. Furthermore, it can serve as a basis for developing corresponding software for multiplex STR typing systems. Developing corresponding analytical software based on this calculation scheme can significantly reduce manual intervention in data analysis, improve the accuracy of analytical results, and significantly reduce experimental workload.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The application discloses a set of detection wheat relative species universal markers and application thereof, and belongs to the technical field of plant genetic engineering. The application takes different species of triticeae as research objects, selects specific primers designed on the basis of the length polymorphism information of different introns of the conserved genes of triticeae identified by using the existing Chinese spring wheat reference genome and annotation information and a bioinformatics method, and finally develops the polymorphic DNA molecular markers capable of distinguishing different ploidy materials of triticeae by using PCR technology and agarose gel electrophoresis technology. The molecular markers have high specificity, stability and universality, and provide a new direction for identification of exogenous chromosome fragments of wheat, molecular marker assisted breeding of exogenous genes and screening of germplasm resources.

The invention relates to the field of plant biotechnology breeding, and discloses a strawberry anther culture regeneration plant optimization method based on multistage screening, which comprises the following steps: constructing a regeneration first generation regeneration plant population through in vitro induction culture; detecting the ploidy of a single plant by using flow cytometry, and removing plants with abnormal ploidy according to a ploidy detection result; calculating a genetic similarity coefficient between the ploidy-stable plant and the parent by using a simple repetitive sequence marker, and removing a genetic variation plant to retain a superior line group; planting the superior line group in a field, and then measuring agronomic, physiological and fruit quality indexes; integrating ploidy, genetic coefficient and multi-dimensional character indexes to establish a grading evaluation model, and dividing the plants into different grades to establish a superior line. By constructing a cell, molecule and phenotype three-level linkage screening system, the screening efficiency and accuracy of strawberry anther culture regeneration plants are improved, and objective quantification of optimal line establishment is realized.

The invention discloses a method for virus-free rapid propagation by utilizing strawberry anther culture, and relates to the technical field of strawberry anther culture, and the method is characterized by comprising the following steps: S1, selection and disinfection of explants, S2, culture stage, S3, ploidy identification, S4, virus detection, and S5, domestication and transplanting of virus-free seedlings. According to the strawberry anther in-vitro culture method, a large number of virus-free plants are successfully obtained through a strawberry anther in-vitro culture technology, the strawberry detoxification efficiency and effect are remarkably improved, and technical support is provided for healthy development of the strawberry industry.

The invention relates to the technical field of plant breeding, discloses a high-quality and high-yield alfalfa directional breeding method and application thereof, and aims to solve the problem that western alfalfa breeding lacks an integrated molecular marker screening system which is combined with a time sequence stress phenotype and can be simultaneously associated with high-protein quality, drought resistance and saline-alkaline characteristics. The method comprises the following steps: constructing association and verification double groups, and performing stress memory activation, ploidy and genetic purity verification and quality preliminary screening; simulating three-stage time sequence stress from a seedling stage to an early overwintering stage, and measuring four-dimensional phenotypic indexes of yield, quality, stress response and habitat adaptation; gWAS analysis is carried out, a double-character co-association marker is mined, and molecular expression, genetic complementation and multi-environment verification are carried out; finally, new germplasm is created through phenotype primary screening, genotype verification, ecological adaptation three-level screening and field trials, the breeding efficiency is improved, the bred variety can stably adapt to the western composite habitat, the high-yield and high-protein characteristics and the soil improvement capacity are both considered, and the field ineffective workload is greatly reduced.

The present application relates to the field of aquatic organism breeding technology, and particularly relates to a method for preparing rainbow trout tetraploid fry. The method provided by the present application comprises the following steps: obtaining fertilized eggs by dry fertilization of naturally matured rainbow trout parents, inducing the rainbow trout fertilized eggs to obtain tetraploid rainbow trout after 6.2-6.4 hours, and the duration of the induction is 10-18 minutes; after the tetraploid rainbow trout fry is obtained by induction, the chromosome ploidy of the fish is determined by using techniques such as chromosome karyotype analysis and flow cytometry DNA content determination, and the tetraploid rainbow trout fry is identified and screened for cultivation. The present application adopts a drug induction method, and controls the starting time and duration of the induction to inhibit the first mitosis time of the fertilized eggs, thereby improving the induction rate and survival rate of the tetraploid rainbow trout, and the induction rate of the tetraploid rainbow trout can reach 25-30%, and the survival rate of the fry can reach more than 90%.

The application discloses a method for creating a DH line of eggplant by using anther culture and application thereof, and relates to the technical field of plant breeding. The application establishes a morphological index of a flower bud based on a calyx lobe separation state, replaces a time-consuming microscope examination procedure, and optimizes a combination of heat treatment and low-temperature pretreatment, so that the embryoid induction rate is significantly improved. Finally, through strict ploidy identification and self-pollination purification, a DH line with consistent traits and stable heredity can be obtained, an efficient pure line source is provided for eggplant hybrid breeding, and efficient conversion from a heterozygous genotype plant to a homozygous DH line is realized.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

This invention discloses a method for creating maize androgenetic haploid inducible lines and its applications. This invention belongs to the field of biotechnology. The substances regulating the expression of protein-coding genes or regulating the activity or content of said proteins can be applied in any of the following ways: U1) application in regulating the induction ability of plant androgenetic haploids; U2) application in preparing products that regulate the induction ability of plant androgenetic haploids; U3) application in cultivating plants with altered androgenetic haploid induction ability; U4) application in preparing products that cultivate plants with altered androgenetic haploid induction ability; U5) application in plant breeding. This invention can identify haploids through the compact, leafless plant phenotype, and further confirm their ploidy using flow cytometry, greatly reducing screening costs and time, and has promising applications.

The invention relates to the technical field of plant genetic breeding, in particular to a directional pyramiding breeding method for improving the pungency degree and color value of processed chilies. Comprising the steps of parent screening (molecular marker identification of high-pungency and high-color-value germplasm based on capsaicin synthesis key genes Pun1 and Capana06g001204 and capsorubin regulatory genes CaBBX20 and CCS), hybridization polymerization (multi-generation backcross and molecular marker-assisted selection integration of pungency degree / color value genes), ploidy breeding accelerated homozygosis (a microspore culture combined chromosome doubling technology), and cross breeding of the high-pungency and high-color-value germplasm. And performing phenotype verification (detecting the content of capsaicin substances by high performance liquid chromatography and determining the color value by spectrophotometry). According to the method, gene modules for controlling the pungency degree and the color value are directionally polymerized, a special dry processing variety with the capsaicin content being larger than or equal to 3% (the corresponding pungency degree being larger than or equal to 30,000 SHU) and the color value being larger than or equal to 300 ASTA is bred, the breeding period is shortened to 1 / 3 of that of a traditional method, and the requirement of the pepper processing industry for high-quality raw materials is met. The method is suitable for large-scale pepper breeding, and is particularly suitable for planting in high-altitude and arid regions.

The invention provides an absolute copy number calculation method, and relates to the field of biological information. According to the method, the purity and ploidy of a sample are determined according to log2Ratio and BAF of a heterozygous embryonic line mutation site. The method has high sensitivity and specificity, overcomes the problems of high cost and long period of whole genome sequencing, can ensure good copy number calculation accuracy even under the condition of extremely low cost, and has good clinical application prospects.

Provided herein are systems and methods for allele-specific copy number (ASCN) calling using sequencing data. The presently described systems and methods can support various configurations based on different sequencing assays and disease contexts. The techniques include segmentation of target regions of interest based on similarity of coverage ratio and B-allele frequency (BAF) values for adjacent target regions, and estimation of an allele-specific copy number for each segment based at least one the observed coverage ratio, an expected coverage ratio, an observer BAF value, and an expected BAF value for each segment, wherein the expected coverage ratio and / or the expected BAF are calculated based on tumor purity, tumor ploidy, and total copy number for each segment.

Techniques for determining whether a significant copy number amplification has occurred are described. An example method includes determining, by analyzing sequence read data of a sample obtained from a subject, a copy number of a segment of the sample; and determining, by analyzing the sequence read data, a ploidy of the sample by determining an average copy number of multiple segments across a genome of the sample. The multiple segments across the genome of the sample include the segment of the sample. The example method further includes determining a ratio by dividing the copy number of the segment of the sample by the ploidy of the sample; comparing the ratio to a threshold; and based on comparing the ratio to the threshold, outputting an indication of the copy number of the segment of the sample.

The present invention relates to the technical field of image detection. In particular, the invention relates to a computer-implemented method, an apparatus, a use, and a program element to determine the ploidy of a vascular plant. The present invention also relates to a computer-implemented method for training an image classifier to determine the ploidy of a vascular plant.

The present application relates to the assisted evaluation method of the second biopsy in the pre-implantation genetic testing. The blastocyst first biopsy process collects and cryopreserves the corresponding biopsy droplet; for the blastocyst with CNV detection failure or suspected abnormal CNV detection result in the first biopsy cell, the first biopsy droplet is thawed to complete genome amplification, CNV library construction, high-throughput sequencing and CNV analysis, and the second biopsy suggestion is given according to the detection result. If the biopsy droplet detects the abnormal aneuploidy or chimera clinically interpreted, it is suggested that the corresponding blastocyst has a high probability of chromosomal abnormality, and the second biopsy is not recommended; if the biopsy droplet does not detect the abnormal aneuploidy or chimera clinically interpreted, it is suggested that the corresponding blastocyst has a high probability of chromosomal normality, and the second biopsy is recommended. The present application proves that the first biopsy droplet contains detectable genetic material, and the consistency of the chromosomal ploidy detection result and the biopsy cell is more than 80%, which can assist the second biopsy decision and improve the decision efficiency and scientificity of PGT detection.

The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.

The present disclosure provides a gene copy number variation type detection method and related products. A specific embodiment of the gene copy number variation type detection method additionally considers the tumor ploidy of the sample to be tested in the process of determining the corrected tumor cell copy number of the gene to be tested in the sample to be tested. Since the factors considered are more abundant, the accuracy of the corrected tumor cell copy number of the gene to be tested in the sample to be tested can be improved. In turn, the accuracy of detecting the copy number variation state of the gene to be tested can be improved.

This invention relates to the field of breeding ploidy anomaly detection technology, specifically to a method for detecting ploidy anomalies in pear breeding. The method includes: performing Hough circle detection on flow cytometry scatter plots corresponding to pear tree leaf tissues to obtain individual Hough circles; obtaining cluster core regions based on the number of scatter points on the edge lines of each Hough circle, the perimeter of the Hough circle, and its area; merging the corresponding regions to be merged with the cluster core regions based on the areal density of the cluster core regions and the scatter points in the regions to be merged, obtaining the region with the highest density; and obtaining the target subgroup based on the number of scatter points in the structural elements corresponding to each edge scatter point and the edge scatter points of the region with the highest density, thereby determining whether the breeding ploidy is abnormal. This invention improves the accuracy of breeding ploidy identification results.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

This invention discloses a method for producing and identifying haploid rice, belonging to the field of biotechnology. The method includes the following steps: (1) using a dominant male-sterile line as the female parent and a haploid inducible line carrying the red fluorescent protein gene mCherry as the male parent for hybridization and pollination to obtain hybrid seeds; (2) after the hybrid seeds germinate, candidate haploid seeds without fluorescence are screened by observing the red fluorescence in the roots and buds; (3) the seedlings obtained from the candidate haploid seeds are identified by flow cytometry. This invention uses a dominant male-sterile line as the hybrid female parent, which improves the efficiency of pollination and hybridization and greatly reduces the amount of manual labor and time; the haploid inducible line combined with fluorescent labeling technology, by screening for non-fluorescent seeds and then performing flow cytometry background measurement to determine ploidy, haploid seedlings can be screened in the early stage, and the screening efficiency is greatly improved, while saving time and field planting workload.