Method for utilizing plant heterosis

A technology of heterosis and plants, applied in the biological field, can solve the problems of consuming manpower, material resources and land resources, the purity of hybrid seeds is not up to standard, and the utilization rate of germplasm resources is low

Active Publication Date: 2019-06-28
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Since hybrid rice utilizes the heterosis in one generation, the separation of traits or fertility will occur in subsequent generations, so it is necessary to produce seeds every year, which consumes a lot of manpower, material resources and land resources.
In addition, the "three-line method" is restricted by the restoration relationship and has a low utilization rate of germplasm resources; the "two-line method" is affected by natural temperature and light, the reproductive output of the sterile line is unstable, and there are low-temperature induced insecurities during hybrid seed production. The risk of substandard hybrid seed purity due to self-fertilization of breeding lines

Method used

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  • Method for utilizing plant heterosis
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  • Method for utilizing plant heterosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0119] According to a typical embodiment of the present invention, the method includes the following steps: S1, using gene mutation or genetic engineering technology to transform the meiosis of the germ cells of the hybrid into similar mitosis to obtain the genotype and chromosomal ploidy of the hybrid identical gametes; and S2, inducing gametes to develop into seeds or plants.

[0120] Applying the technical solution of the present invention, the hybrid can produce cloned seeds or plants that are completely consistent with its own genotype, so that the hybrid can be used for a long time, and solve the problem of crossing between parents due to inconsistent flowering periods and other reasons in the use of heterosis. low yield and high cost of hybrids.

[0121] According to a typical embodiment of the present invention, S1 includes taking a hybrid, using genetic mutation or genetic engineering technology to convert the meiosis of its germ cells to similar to mitosis, so as to ...

Embodiment 1

[0205] 1. In this example, the F 1 The hybrid is the approved and commercialized hybrid rice variety Chunyou 84. Chunyou 84 is a new japonica-non-indica-recovery interspecies hybrid rice combination bred by the early-flowering and late-japonica CMS line Chunjiang 16A and the indica-japonica intermediary wide-compatibility restorer line C84. The hybrid rice has the advantages of large yield potential, high seed production yield, excellent comprehensive agronomic traits, good blast resistance, wide adaptability and the like. The genetic transformation background material used in this example is the hybrid rice F 1 The callus obtained by seed induction has not gone through the sexual reproduction stage, therefore, the transgenic T 0 The genetic background of the generation material is similar to that of the hybrid rice F 1 Plants consistent.

[0206] 2. Construction of multiple gene knockout vectors.

[0207] The main steps are as follows (see also CN201510485573.2 for detai...

Embodiment 2

[0239] 1. In this example, the maintainer line Chunjiang 16B and the intermediary indica-japonica wide-compatibility restorer line C84 were used. The background material of genetic transformation used in this example is the callus induced by parental seeds.

[0240] 2. Construction of multiple gene knockout vectors.

[0241] The main steps are as follows

[0242] 1) Construction of a single target SK-gRNA:

[0243] The following four sites were selected as the sites for knocking out REC8, OSD1, PAIR1 and MTL by the CRISPR-Cas9 gene editing system (the PAM sequence indicated by the underline):

[0244] OSD1 gene knockout site (SEQ ID NO: 1): CTGCCGCCGACGAGCAACA AGG

[0245] PAIR1 gene knockout site (SEQ ID NO: 2): AAGCAACCCAGTGCACCGC TGG

[0246] REC8 gene knockout site (SEQ ID NO: 3): CCCATGGCACTAAGGCTCT CCG

[0247] MTL gene knockout site (SEQ ID NO: 4): GGTCAACGTCGAGACCGGC AGG

[0248] Design two complementary DNA sequences: add GGCA before the forward sequence...

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Abstract

The invention discloses a method for utilizing plant heterosis. The method comprises the following steps: S1, converting meiosis of germ cells of a hybrid into similar mitosis by using a gene mutationor genetic engineering technology so as to obtain gametes consistent with a genotype and chromosome ploidy of the hybrid; and S2, inducing the gametes to develop into seeds or plants. By applying thetechnical scheme of the invention, hybrid seeds can generate cloned seeds or plants which are completely consistent with its genotype and chromosome ploidy, so that the heterosis can be utilized fora long time, and the existing problems of difficulty in parent-to-parent hybridization, low seed production yield, high hybrid seed cost and the like caused by inconsistent flowering periods and the like in the utilization of the heterosis are solved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for utilizing plant heterosis. Background technique [0002] Heterosis refers to the phenomenon that in the biological world, two varieties with different genetic bases or similar species are crossed, and the offspring of the hybrid are superior to their parents in terms of growth vigor, vitality, adaptability and yield. Heterosis is a common phenomenon in the biological world, which is widely used in the breeding and production of crops. [0003] For the application of heterosis in agricultural production, one of the most important links is the efficient preparation of hybrids. In dioecious crops such as corn, the male flowers of the female inbred line can be manually (or mechanically) removed, and the pollen of another inbred line (male parent) can be pollinated to obtain hybrid seeds. The operation is relatively simple and easy. Yes, so the heterosis of maize is used ea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H1/02A01H6/46
CPCC12N15/8216A01H4/008C07K14/415A01H1/12A01H1/08A01H6/46C07K14/475C12N2310/20A01H1/021A01H5/10A01H6/00A01H1/026C12N15/8201
Inventor 王克剑王春
Owner CHINA NAT RICE RES INST
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