Patents
Literature
Patsnap Eureka AI that helps you search prior art, draft patents, and assess FTO risks, powered by patent and scientific literature data.

219 results about "Broodstock" patented technology

Broodstock, or broodfish, are a group of mature individuals used in aquaculture for breeding purposes. Broodstock can be a population of animals maintained in captivity as a source of replacement for, or enhancement of, seed and fry numbers. These are generally kept in ponds or tanks in which environmental conditions such as photoperiod, temperature and pH are controlled. Such populations often undergo conditioning to ensure maximum fry output. Broodstock can also be sourced from wild populations where they are harvested and held in maturation tanks before their seed is collected for grow-out to market size or the juveniles returned to the sea to supplement natural populations. This method, however, is subject to environmental conditions and can be unreliable seasonally, or annually. Broodstock management can improve seed quality and number through enhanced gonadal development and fecundity.

Industrialized cultivation method for cephalopholissonnerati fry

The present disclosure relates to the technical field of marine fish cultivation and breeding, and in particular to an industrialized cultivation method for a cephalopholissonnerati fry. The method includes: a step of sequentially performing food acclimation and nutrient enrichment on a cephalopholissonnerati parent fish under conditions of 25-29° C. of water temperature and running water, to obtain the parent fish with mature gonad development, and other steps.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI +1

Industrialized cultivation method for cephalopholissonnerati fry

The present disclosure relates to the technical field of marine fish cultivation and breeding, and in particular to an industrialized cultivation method for a Cephalopholissonnerati fry. The method includes: a step of sequentially performing food acclimation and nutrient enrichment on a Cephalopholissonnerati parent fish under conditions of 25-29° C. of water temperature and running water, to obtain the parent fish with mature gonad development, and other steps.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI +1

Seedling cultivation method for Jinhu hybrid spots

The invention provides a method for cultivating hybrid fries of Jinhu and tiger. The method comprises the following steps: selecting a breeding environment; breeding and ripening female parents; management and control before oviposition; insemination; hatching fertilized eggs; fry rearing. According to the method, in the female parent breeding and ripening stage, specific biological bait is controlled to be fed in stages, feeding of the parent fish can be promoted, growth can be accelerated, in cooperation with specific compound feed in the management and control stage before oviposition, favorable conditions are created for subsequent oviposition, and the quality and number of ova can be improved. Low-frequency ultrasonic assisted fertilization is introduced in the artificial insemination stage, so that the activity of ovum sperms can be obviously improved, and the fertilization rate is increased; in the fertilized egg hatching stage, intermittent ultrasound is assisted, the hatching rate can be increased, and the seedling yield of the golden-tiger hybrid spots can be increased; after hatching, transferring into a specific culture pond, slowly releasing beneficial bacteria, improving the adaptability of the fry, adsorbing harmful substances, reducing pathogenic bacteria, stabilizing water quality, promoting the growth of the fry, improving the survival rate and reducing the malformation rate.
Owner:陵水晨海种业有限公司

Artificial breeding method of wild sal-family large yellow croaker

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.
Owner:GUANGDONG OCEAN UNIVERSITY

Artificial breeding method of epinephelus trifoliatus and epinephelus lanceolatus hybrid

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA

Method for breeding all-male hybrid pelteobagrus fulvidraco through hybridization of double super-male populations

The invention provides a method for breeding all-male hybrid pelteobagrus fulvidraco through hybridization of double super-male populations, which comprises the following steps: S1, respectively constructing super-male maintenance lines of pelteobagrus fulvidraco and pelteobagrus vachelli by utilizing a sex reversal technology and a sex molecular marker; s2, carrying out artificial propagation on the maintenance line, and propagating the super male fish in batches; s3, partially replacing estrogen with an androgen antagonist for the propagated supermale fish, and synergistically inducing the supermale fish to obtain superfemale fish; s4, performing specific nutrient enrichment cultivation on the super female fish and the super male parent fish; and S5, carrying out reciprocal cross artificial propagation on the super-female fish and the super-male fish between the two super-male groups to obtain a heterozygous super-male group, namely commercial fry of the all-male hybrid pelteobagrus fulvidraco. According to the technical scheme, super-female fishes can be efficiently induced in batches, high-density breeding of parent fishes is achieved, and the effect and efficiency of artificial breeding are remarkably improved; the bred hybrid all-realgar catfish is high in individual uniformity, the growth speed is increased by 20%, and the male rate reaches 100%.
Owner:ZHAOQING JIAYI AQUATIC TECHNOLOGY CO LTD +2

Artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA

Artificial propagation device for light red ink head fish

The utility model discloses an artificial breeding device for light red ink head fish, which belongs to the technical field of aquatic product hatching and fry breeding and comprises a spawning induction pond, a hatching pond and a fry collecting pond. Cobblestones are laid at the bottom of the spawning induction pond, a spraying mechanism is fixedly connected to the top of the spawning induction pond, an ovulation pipe is communicated with the center of the spawning induction pond, the top of the ovulation pipe is lower than the top face of the spawning induction pond, and the bottom end of the ovulation pipe penetrates through the spawning induction pond to be communicated with the hatching pond; a filtering mechanism is detachably connected to the middle of the hatching pond, an overflow pipe is arranged in the filtering mechanism, and the bottom of the overflow pipe penetrates through the hatching pond to communicate with the outside; the side wall of the bottom of the hatching pond is communicated with a seedling outlet pipe, and the seedling outlet pipe is fixedly connected with a control valve and communicated with the seedling collecting pond. The device can effectively simulate the natural breeding environment of the light red ink head fish, accurately control various key parameters in the breeding process, and improve the reproductive performance of parent fish, the hatching rate of fish eggs and the survival rate of fish fries.
Owner:GUANGXI ACAD OF SCI +1

Indoor full-artificial breeding method for garrupa nigatosa

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA

Artificial propagation method of Sinilabeo wuyi

The invention provides an artificial propagation method of Sinilabeo wuyi, and belongs to the technical field of aquatic animal breeding. The artificial propagation method of the Sinilabeo wuyi comprises the steps of parent fish classification, parent fish rearing, artificial spawning induction, natural insemination, artificial insemination, fertilized egg incubation, fry rearing, juvenile fish rearing and the like. The oxytocic is reasonable in compatibility, the injection time of the oxytocic is accurate, the oxytocic rate, the fertilization rate and the hatching rate are increased, and damage to parent fishes is small. According to the method, feeding methods in different stages of fry breeding are also defined, the survival rate of the sinilabeo wuyi fry breeding stage is increased, and a technical support is laid for artificial breeding of the endangered species sinilabeo wuyi.
Owner:WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI +1

Method for efficiently maturing ovaries of industrially cultured plectropomus leopardus

The invention discloses a method for efficient maturation of ovaries of industrially cultured plectropomus leopardus. The method comprises the following steps: screening healthy plectropomus leopardus individuals from a plectropomus leopardus population as parent fishes; putting the fish in an indoor culture pond for water adaptation to obtain high-quality parent fish; performing induced culture on the high-quality parent fish, wherein the induced culture comprises nutrient enrichment and temperature induction; the temperature induction time is less than or equal to 35 days to promote the ovary maturation of the parent fish. Through an indoor induction mode combining low-temperature energy storage, gradient rewarming and nutrient enrichment feed, the maturation rate of the female plectropomus leopardus ovary can be remarkably increased to 85% or above, and the maturation period is shortened to be within 40 days. The method is suitable for a standardized breeding process of an indoor parent fish breeding workshop, effectively realizes synchronous maturation and quality improvement of the parent fish group ovary, provides a stable parent guarantee for indoor artificial breeding of plectropomus leopardus, and has a good popularization and application prospect.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA

Polypeptide with ovary maturation promoting effect and application of polypeptide in pomfret spawning induction

The invention provides a polypeptide with an ovary maturation promoting effect and application of the polypeptide in parturition induction of pomfret. The amino acid sequence of the polypeptide is SEQ ID NO: 1. The polypeptide provided by the invention is derived from the GnRH2 gene of the pomfret, and in-vivo experiments prove that the polypeptide can efficiently stimulate the pituitary of the pomfret to secrete gonadotropin, so that the spawning induction time is remarkably shortened, and the spawning rate and the relative spawning amount of parent fish are increased. The polypeptide is strong in species specificity and good in physiological compatibility, does not generate an antibody, avoids side effects of traditional heterologous hormones, is safe and effective, and has a huge potential of being developed into a special spawning induction agent for the pomfret.
Owner:NINGBO UNIV

Method for regulating out-of-season breeding of large yellow croakers bred in autumn

The invention discloses a method for regulating out-of-season breeding of large yellow croakers bred in autumn. The method is characterized in that spawning in spring and summer is achieved through a three-stage technical system of biological safety prevention and control, precise environment regulation and control and reproductive physiological intervention. The method specifically comprises the following steps: 1) detecting trypanosomes; 2) parent fish screening; (3) parent fish rearing; 4) artificial propagation; 5) artificial incubation; and 6) seedling cultivation. According to the method, the out-of-season spring and summer breeding technology breakthrough of breeding the large yellow croaker in autumn is successfully achieved through nutrient enrichment, accurate temperature parameter regulation and control, gradual illumination regulation and reasonable hormone treatment. According to the technology, an adjustable reproduction synchronization scheme is provided for cross breeding between a spring reproduction group and an autumn reproduction group, and the technology has important application value for germplasm innovation of the large yellow croaker.
Owner:ZHEJIANG OCEAN UNIV

Breeding method of fast-growing high-quality Dongtaotai

The invention provides a breeding method of fast-growing high-quality Dongzhihuo. Comprising the following steps: identifying the genotype of an SNP (Single Nucleotide Polymorphism) locus which is located at the 79th site of a gene sequence as shown in SEQ ID NO.1, and if the genotype of the locus is TT type, determining that the locus is a candidate parent; selecting candidate parents with uniform and robust body types as parent fishes; carrying out spawning induction on the male fish and the female fish of the Dongstellate star; collecting ova and sperms for artificial insemination; uniformly putting the fertilized eggs into an indoor nursery pond for hatching and breeding. The euryale ferox bred through the method is high in growth and development speed, high in muscle fat content, high in crude protein content, high in flavor amino acid content, delicious in taste, more popular with consumers and good in market prospect.
Owner:HAINAN CHENHAI AQUATIC CO LTD

Method for mass production of all-female triploid mandarin fish

PendingCN122319994AHeat shockAnimal science
This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.
Owner:SUN YAT SEN UNIV

River perch sex specific molecular marker and application thereof

The invention belongs to the technical field of molecular biology and aquaculture, and discloses a river perch sex specific molecular marker and application thereof. The molecular marker provided by the invention is directly derived from a sex specific region of the river perches and is closely linked with sex phenotypes, the identification result is accurate and reliable, and the accuracy rate can reach 100%. Enough DNA can be extracted for detection only by using a small amount of tissue samples (such as fin rays, scales, muscles or blood) of the river perches, so that the method is harmless to parent fishes, and the intactness of breeding materials is ensured. Powerful technical support is provided for all-male / all-female breeding, family construction, growth and sex relationship research and breeding production management of the river perches, and the method has a wide application prospect.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

A circulating water culture system for indoor multi-layer culture of freshwater fish germplasm samples

This invention belongs to the field of aquaculture equipment and discloses a recirculating aquaculture system for indoor multi-layered culture of freshwater fish germplasm samples. The system includes a stainless steel culture rack, glass culture tanks, a temperature control system, a recirculating water purification system, an aeration system, and electrical and auxiliary materials. Through a three-layered culture structure design, combined with culture tanks of varying sizes and a precise temperature control module, it solves the problem of aquaculture with diverse sizes of freshwater fish germplasm samples and large differences in suitable water temperatures. The system has nine small glass culture tanks in the upper layer and three large glass culture tanks in each of the middle and lower layers, equipped with an external temperature control component and an internal heating rod, achieving full-coverage water temperature regulation from 10℃ to above 30℃. The recirculating water system ensures stable water quality through the synergistic effect of a filter tank, a booster pump, an ultraviolet sterilizer, and various filter media. Meanwhile, the lower culture tanks are equipped with an internal circulation filter to enhance local water purification, meeting the diverse aquaculture needs from small fry to large broodstock.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Industrialized cultivation method for cephalopholis sonnerati fry

PCT designated stageWO2026011334A1Climate change adaptationPisciculture and aquariaCephalopholis sonneratiBroodstock
The present invention relates to the technical field of aquaculture and breeding of seawater fishes, in particular to an industrialized cultivation method for cephalopholis sonnerati fry, comprising the steps of successively subjecting parent fishes of cephalopholis sonnerati to feed domestication and nutrient enrichment at a water temperature of 25-29°C and under a flowing water condition, so as to obtain parent fishes with fully developed gonads, etc. The present invention takes strict measures for fry quality control and infectious disease prevention and control during industrialized workshop cultivation, so as to effectively optimize the growth conditions of fry, thus increasing the growth rate and improves the health conditions and adaptability of fry while reducing the risk of diseases. By means of standardized breeding management and technical means, the growth environment and feeding management of fry can be controlled, thus effectively improving production efficiency. The cultivated cephalopholis sonnerati has the advantages of fast growth rate, high disease resistance, strong vigor, tender and delicious flesh and good market prospects.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI +1

A method for evaluating the survival ability of scallops

The present invention discloses a method for evaluating the survival ability of scallops, which can be used as a new method for the selection and breeding of scallop broodstock and the evaluation of aquaculture. This method monitors the process of the opening and closing movement of the two valves of scallops in vitro, analyzes the law of the opening and closing movement of scallops, calculates the opening and closing movement ability of scallops, and evaluates and predicts the survival potential of scallops. Compared with the traditional method, this method has the characteristics of non-invasiveness, non-damage, and strong timeliness, can evaluate the survival potential of live shellfish, and does not harm the target scallops. The present invention provides a new method for predicting the survival potential of scallops, and the application of this method will provide technical support for the selection and breeding of scallop broodstock with high survival ability and the healthy aquaculture of shellfish.
Owner:OCEANOGRAPHIC INSTR RES INST SHANDONG ACAD OF SCI

Method for breeding and breeding squaliobarbus curriculus based on aquaculture

The invention discloses a squaliobarbus curriculus breeding and breeding method based on aquaculture. According to the method, slow flow domestication is started from the fry stage, the fries are made to adapt to the dynamic water environment from small, the physique is obviously enhanced, the fries are not prone to being infected with diseases during adult fish culture, and the trouble and cost of medication are reduced. In addition, through the feed transition domestication in the S3, the palatability of the fries eating the compound feed in the later stage is better, the growth speed is more uniform, the subsequently screened water-infused fishes are neat in specification, the water-infused fishes can more quickly adapt to fasting and water flow environments during water infusing, the weight-reducing and fishy smell-removing effects are more stable, the meat quality is compact and elastic, the mud fishy smell basically disappears, and the quality and the appearance of the fishes are directly improved; a cycle is formed from parent fish selective culture to sales feedback, the parent fish breeding direction and the adult fish culture density can be adjusted in time according to the requirements of the market end, the unsalable risk caused by blind culture is avoided, and the income of farmers is guaranteed.
Owner:JIAYING UNIV

Multifunctional fish breeding and culturing facility

The utility model provides a multifunctional fish breeding and culturing facility. The multifunctional fish breeding and culturing facility comprises a hatching spraying facility, a movable partition plate, a culturing water inlet pipe, a culturing tank body, a hatching frame and a culturing water outlet pipe, the depth of the cylinder body is gradually increased along the water flow direction; partition plate inserting grooves are formed in the inner wall of the breeding tank body at intervals in the water flow direction, and the movable partition plate is inserted into each partition plate inserting groove; the breeding space of the breeding tank body is divided into a plurality of breeding subareas through the movable partition plates. According to the utility model, intensive culture facilities are adopted to solve the problems of viscous fertilized egg incubation, culture of fishes with different habits and partitioned culture of fishes with different specifications in different stages, so that the production and culture in the whole stage from parent fish culture, parent fish spawning and fertilization, fertilized egg incubation to open fry culture, fry culture and fish culture are realized; the artificial injury to the fishes caused by frequent conversion of culture facilities in the culture process is avoided, and the culture efficiency and effect of the fishes are improved.
Owner:POWERCHINA BEIJING ENG CORP

Bionic efficient breeding method for micropterus salmoides offspring seeds

The invention relates to the technical field of fry breeding in aquaculture, and discloses a micropterus salmoides fry bionic efficient breeding method which comprises the following steps: step 1, selecting parents, and performing physique exercise and optimization on the parents through industrial water tank culture in a pond; 2, preferably selecting parents, and carrying out winter intensified cultivation in an indoor temperature-controlled overwintering pond; thirdly, nutrient enrichment and gonad ripening acceleration of the parents are achieved by feeding animal bait and adjusting water flow; step 4, performing simulated ecological environment construction and parent fish breeding in the outdoor soil pond; 5, fertilized egg incubation is conducted under the indoor controllable condition; sixthly, large-scale fry breeding is carried out in the indoor temperature control cement pond. According to the micropterus salmoides fry bionic efficient breeding method, the micropterus salmoides fry breeding efficiency can be remarkably improved, the fry quality can be improved, the labor intensity and the cost input can be effectively reduced, and micropterus salmoides fry production and commercial fish breeding industry development can be powerfully promoted.
Owner:HUAIYIN TEACHERS COLLEGE

Method for on-site rapid artificial insemination of wild pseudosciaena crocea

The application provides a method for on-site rapid artificial insemination of wild Pseudosciaena crocea of Lutjanidae, and belongs to the technical field of aquatic animal breeding. Wild parent fish of Pseudosciaena crocea of Lutjanidae are captured in the breeding season; sperm is collected from the selected sexually mature male parent fish on the capture site; after artificial sperm collection, sexually mature female parent fish are selected, and fish eggs are collected; high-quality fertilized eggs are obtained through artificial insemination; the artificial insemination comprises the following steps: adding sperm into fish eggs, adding natural seawater for stirring, and finally washing with natural seawater. Through comprehensive regulation of key links in the whole process of artificial insemination, the purpose of obtaining high-quality fertilized eggs by using wild parent fish of Pseudosciaena crocea of Lutjanidae and carrying out artificial fry breeding is achieved.
Owner:GUANGDONG OCEAN UNIVERSITY

Artificial breeding method of Lancang schizothorax

This invention discloses an artificial breeding method for *Schizothorax lancensis*, belonging to the field of aquatic animal breeding technology. The artificial breeding method for *Schizothorax lancensis* provided by this invention includes: intensive three-step cultivation of broodstock, injection of spawning induced agents after cultivation, artificial fertilization, hatching of fertilized eggs, and fry cultivation. Using the artificial breeding method of this invention, the success rate of spawning induction in female *Schizothorax lancensis* is 83.3%, the average fertilization rate is 87.5%, the average hatching rate is 93.0%, and the average fry emergence rate is 91.5%. This achieves large-scale artificial breeding of *Schizothorax lancensis*, laying the foundation for preventing the extinction of *Schizothorax lancensis*, and for carrying out the propagation and release of *Schizothorax lancensis* for industrial utilization.
Owner:HUANENG LANCANG RIVER HYDROPOWER CO LTD +1

A method for efficiently inducing heteropolyploid fry

ActiveCN118947633BHeat shockAnimal science
This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.
Owner:HUNAN NORMAL UNIVERSITY

Artificial breeding method of lota lota

The invention discloses an artificial breeding method of lota lota, which comprises the following steps: S1, selecting robust lota lota as parent fish, placing the parent fish in an indoor culture tank for temporary culture, and inducing gonad to develop mature by regulating and controlling water temperature; s2, performing artificial spawning induction on the parent fish with mature gonad to obtain ova and semen, and performing in-vitro fertilization on the ova and the semen to obtain fertilized ova; s3, performing two-step soaking treatment on the fertilized eggs by using a composite biological functional liquid containing a liquid A and a liquid B, and then transferring the fertilized eggs into a hatching facility for hatching to obtain fish fries; and S4, breeding the hatched fish fries. According to the artificial breeding method of the lota lota, the survival rate of fertilized eggs and the survival rate in the fry breeding stage are increased to a high level, large-scale and controllable artificial breeding of the lota lota becomes possible, and the overall technical scheme has high stability and industrial application value.
Owner:WUHAN SINO-SCI RUIHUA ECO TECH CO LTD +3

Feed and method for promoting ovary maturation of scatophagus argus

The invention discloses a feed and method for promoting ovaries of scatophagus argus to be mature, the feed comprises a feed 1, a feed 2 and a feed 3, after parent fish is temporarily bred in net cages, first-stage domestication is started in the net cages, the feed 1 is fed during the period, dysplastic individuals are removed after the period is finished, second-stage domestication is started in the net cages, the feed fed during the period is a mixture of the feed 1 and the feed 2, and the feed 3 is a mixture of the feed 1 and the feed 2. And after finishing feeding, rejecting the individuals with dysplasia, starting domestication at the third stage in the net cage, and feeding the feed 3 during the domestication. According to the method, the maturation of the ovary of the female fish can be remarkably promoted, the maturation of the ovary of the female fish is advanced by 2 months, synchronous sexual maturation with the male fish of the same age is achieved, the ovulation amount of the parent fish is excellent, the fertilization rate is high, and the development of the scatophagus argus artificial breeding technology can be effectively promoted.
Owner:GUANGDONG OCEAN UNIVERSITY

Whole-process breeding method and system for Pseudosciaena crocea of sal family

The invention relates to the field of fish breeding, and discloses a full-process breeding method and system for Pseudosciaena of the Halitium family, and the method comprises the following steps: carrying out state analysis and screening on Pseudosciaena of the Halitium family to obtain target parent fish, carrying out bait feeding and oxytocic injection on the target parent fish to ensure that the target parent fish is in a sexual maturity state, and breeding the Pseudosciaena of the Halitium family to obtain the Pseudosciaena of the Halitium family. Roes of the target parent fish in the sexual maturity state are collected, and meanwhile water quality parameter control is conducted on the environment where the target parent fish is located. According to the breeding method, the breeding habit of the Halitium pseudosciaena crocea is understood, the research of intensive breeding of the parent fish is combined, the method for ripening and spawning of the parent fish of the Halitium pseudosciaena crocea is explored, full-process breeding and breeding of the Halitium pseudosciaena crocea in the Xiaozhou family are achieved, the problem of resource exhaustion of the Halitium pseudosciaena crocea in the Xiaozhou family is solved, and a foundation is provided for large-scale production of fry of the Halitium
Owner:广东省农业技术推广中心

Breeding method of hypoxia-resistant grouper

The invention discloses a breeding method of anti-hypoxia grouper, and belongs to the technical field of grouper breeding. The method comprises the steps of parent fish selection, hypoxia domestication, juvenile fish screening, SNP molecular marker assisted breeding and the like, and finally the hypoxia-resistant grouper variety is obtained. By means of the method, the grouper variety high in hypoxia resistance can be cultivated, the cultivation cost is reduced, and sustainable development of the grouper cultivation industry is promoted.
Owner:HAINAN CHENHAI AQUATIC CO LTD

Grass carp intensive high-yield character genome selective breeding method

The invention belongs to the technical field of fish genetic breeding in aquaculture industry, and discloses a grass carp intensive high-yield character genome selective breeding method, which comprises the following steps: establishing a grass carp intensive high-yield character prediction model; the establishment of the grass carp intensive high-yield character prediction model comprises the following steps: (1) obtaining a grass carp candidate parent fish single nucleotide polymorphism site; (2) constructing an analysis model for predicting breeding values of candidate parents; and (3) constructing a molecular genetic relationship matrix by using the obtained single nucleotide polymorphic sites, and predicting a genome estimation breeding value of the candidate group in combination with the analysis model in the step (2). The improved variety breeding method based on grass carp genome information can be used for screening excellent parents suitable for intensive breeding, the screened parents are directly used for breeding offspring seeds, and the high-density breeding tolerance of grass carp can be directly improved; and a technical support is provided for improving cultivation of new grass carp varieties suitable for intensive and facility cultivation.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI