164 results about "Broodstock" patented technology

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention provides a method for breeding all-male hybrid pelteobagrus fulvidraco through hybridization of double super-male populations, which comprises the following steps: S1, respectively constructing super-male maintenance lines of pelteobagrus fulvidraco and pelteobagrus vachelli by utilizing a sex reversal technology and a sex molecular marker; s2, carrying out artificial propagation on the maintenance line, and propagating the super male fish in batches; s3, partially replacing estrogen with an androgen antagonist for the propagated supermale fish, and synergistically inducing the supermale fish to obtain superfemale fish; s4, performing specific nutrient enrichment cultivation on the super female fish and the super male parent fish; and S5, carrying out reciprocal cross artificial propagation on the super-female fish and the super-male fish between the two super-male groups to obtain a heterozygous super-male group, namely commercial fry of the all-male hybrid pelteobagrus fulvidraco. According to the technical scheme, super-female fishes can be efficiently induced in batches, high-density breeding of parent fishes is achieved, and the effect and efficiency of artificial breeding are remarkably improved; the bred hybrid all-realgar catfish is high in individual uniformity, the growth speed is increased by 20%, and the male rate reaches 100%.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The utility model discloses an artificial breeding device for light red ink head fish, which belongs to the technical field of aquatic product hatching and fry breeding and comprises a spawning induction pond, a hatching pond and a fry collecting pond. Cobblestones are laid at the bottom of the spawning induction pond, a spraying mechanism is fixedly connected to the top of the spawning induction pond, an ovulation pipe is communicated with the center of the spawning induction pond, the top of the ovulation pipe is lower than the top face of the spawning induction pond, and the bottom end of the ovulation pipe penetrates through the spawning induction pond to be communicated with the hatching pond; a filtering mechanism is detachably connected to the middle of the hatching pond, an overflow pipe is arranged in the filtering mechanism, and the bottom of the overflow pipe penetrates through the hatching pond to communicate with the outside; the side wall of the bottom of the hatching pond is communicated with a seedling outlet pipe, and the seedling outlet pipe is fixedly connected with a control valve and communicated with the seedling collecting pond. The device can effectively simulate the natural breeding environment of the light red ink head fish, accurately control various key parameters in the breeding process, and improve the reproductive performance of parent fish, the hatching rate of fish eggs and the survival rate of fish fries.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention provides an artificial propagation method of Sinilabeo wuyi, and belongs to the technical field of aquatic animal breeding. The artificial propagation method of the Sinilabeo wuyi comprises the steps of parent fish classification, parent fish rearing, artificial spawning induction, natural insemination, artificial insemination, fertilized egg incubation, fry rearing, juvenile fish rearing and the like. The oxytocic is reasonable in compatibility, the injection time of the oxytocic is accurate, the oxytocic rate, the fertilization rate and the hatching rate are increased, and damage to parent fishes is small. According to the method, feeding methods in different stages of fry breeding are also defined, the survival rate of the sinilabeo wuyi fry breeding stage is increased, and a technical support is laid for artificial breeding of the endangered species sinilabeo wuyi.

The invention discloses a method for efficient maturation of ovaries of industrially cultured plectropomus leopardus. The method comprises the following steps: screening healthy plectropomus leopardus individuals from a plectropomus leopardus population as parent fishes; putting the fish in an indoor culture pond for water adaptation to obtain high-quality parent fish; performing induced culture on the high-quality parent fish, wherein the induced culture comprises nutrient enrichment and temperature induction; the temperature induction time is less than or equal to 35 days to promote the ovary maturation of the parent fish. Through an indoor induction mode combining low-temperature energy storage, gradient rewarming and nutrient enrichment feed, the maturation rate of the female plectropomus leopardus ovary can be remarkably increased to 85% or above, and the maturation period is shortened to be within 40 days. The method is suitable for a standardized breeding process of an indoor parent fish breeding workshop, effectively realizes synchronous maturation and quality improvement of the parent fish group ovary, provides a stable parent guarantee for indoor artificial breeding of plectropomus leopardus, and has a good popularization and application prospect.

The invention provides a polypeptide with an ovary maturation promoting effect and application of the polypeptide in parturition induction of pomfret. The amino acid sequence of the polypeptide is SEQ ID NO: 1. The polypeptide provided by the invention is derived from the GnRH2 gene of the pomfret, and in-vivo experiments prove that the polypeptide can efficiently stimulate the pituitary of the pomfret to secrete gonadotropin, so that the spawning induction time is remarkably shortened, and the spawning rate and the relative spawning amount of parent fish are increased. The polypeptide is strong in species specificity and good in physiological compatibility, does not generate an antibody, avoids side effects of traditional heterologous hormones, is safe and effective, and has a huge potential of being developed into a special spawning induction agent for the pomfret.

The invention discloses a method for regulating out-of-season breeding of large yellow croakers bred in autumn. The method is characterized in that spawning in spring and summer is achieved through a three-stage technical system of biological safety prevention and control, precise environment regulation and control and reproductive physiological intervention. The method specifically comprises the following steps: 1) detecting trypanosomes; 2) parent fish screening; (3) parent fish rearing; 4) artificial propagation; 5) artificial incubation; and 6) seedling cultivation. According to the method, the out-of-season spring and summer breeding technology breakthrough of breeding the large yellow croaker in autumn is successfully achieved through nutrient enrichment, accurate temperature parameter regulation and control, gradual illumination regulation and reasonable hormone treatment. According to the technology, an adjustable reproduction synchronization scheme is provided for cross breeding between a spring reproduction group and an autumn reproduction group, and the technology has important application value for germplasm innovation of the large yellow croaker.

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

The invention belongs to the technical field of molecular biology and aquaculture, and discloses a river perch sex specific molecular marker and application thereof. The molecular marker provided by the invention is directly derived from a sex specific region of the river perches and is closely linked with sex phenotypes, the identification result is accurate and reliable, and the accuracy rate can reach 100%. Enough DNA can be extracted for detection only by using a small amount of tissue samples (such as fin rays, scales, muscles or blood) of the river perches, so that the method is harmless to parent fishes, and the intactness of breeding materials is ensured. Powerful technical support is provided for all-male / all-female breeding, family construction, growth and sex relationship research and breeding production management of the river perches, and the method has a wide application prospect.

This invention belongs to the field of aquaculture equipment and discloses a recirculating aquaculture system for indoor multi-layered culture of freshwater fish germplasm samples. The system includes a stainless steel culture rack, glass culture tanks, a temperature control system, a recirculating water purification system, an aeration system, and electrical and auxiliary materials. Through a three-layered culture structure design, combined with culture tanks of varying sizes and a precise temperature control module, it solves the problem of aquaculture with diverse sizes of freshwater fish germplasm samples and large differences in suitable water temperatures. The system has nine small glass culture tanks in the upper layer and three large glass culture tanks in each of the middle and lower layers, equipped with an external temperature control component and an internal heating rod, achieving full-coverage water temperature regulation from 10℃ to above 30℃. The recirculating water system ensures stable water quality through the synergistic effect of a filter tank, a booster pump, an ultraviolet sterilizer, and various filter media. Meanwhile, the lower culture tanks are equipped with an internal circulation filter to enhance local water purification, meeting the diverse aquaculture needs from small fry to large broodstock.

The present invention relates to the technical field of aquaculture and breeding of seawater fishes, in particular to an industrialized cultivation method for cephalopholis sonnerati fry, comprising the steps of successively subjecting parent fishes of cephalopholis sonnerati to feed domestication and nutrient enrichment at a water temperature of 25-29°C and under a flowing water condition, so as to obtain parent fishes with fully developed gonads, etc. The present invention takes strict measures for fry quality control and infectious disease prevention and control during industrialized workshop cultivation, so as to effectively optimize the growth conditions of fry, thus increasing the growth rate and improves the health conditions and adaptability of fry while reducing the risk of diseases. By means of standardized breeding management and technical means, the growth environment and feeding management of fry can be controlled, thus effectively improving production efficiency. The cultivated cephalopholis sonnerati has the advantages of fast growth rate, high disease resistance, strong vigor, tender and delicious flesh and good market prospects.

The invention discloses a squaliobarbus curriculus breeding and breeding method based on aquaculture. According to the method, slow flow domestication is started from the fry stage, the fries are made to adapt to the dynamic water environment from small, the physique is obviously enhanced, the fries are not prone to being infected with diseases during adult fish culture, and the trouble and cost of medication are reduced. In addition, through the feed transition domestication in the S3, the palatability of the fries eating the compound feed in the later stage is better, the growth speed is more uniform, the subsequently screened water-infused fishes are neat in specification, the water-infused fishes can more quickly adapt to fasting and water flow environments during water infusing, the weight-reducing and fishy smell-removing effects are more stable, the meat quality is compact and elastic, the mud fishy smell basically disappears, and the quality and the appearance of the fishes are directly improved; a cycle is formed from parent fish selective culture to sales feedback, the parent fish breeding direction and the adult fish culture density can be adjusted in time according to the requirements of the market end, the unsalable risk caused by blind culture is avoided, and the income of farmers is guaranteed.

The application provides a method for on-site rapid artificial insemination of wild Pseudosciaena crocea of Lutjanidae, and belongs to the technical field of aquatic animal breeding. Wild parent fish of Pseudosciaena crocea of Lutjanidae are captured in the breeding season; sperm is collected from the selected sexually mature male parent fish on the capture site; after artificial sperm collection, sexually mature female parent fish are selected, and fish eggs are collected; high-quality fertilized eggs are obtained through artificial insemination; the artificial insemination comprises the following steps: adding sperm into fish eggs, adding natural seawater for stirring, and finally washing with natural seawater. Through comprehensive regulation of key links in the whole process of artificial insemination, the purpose of obtaining high-quality fertilized eggs by using wild parent fish of Pseudosciaena crocea of Lutjanidae and carrying out artificial fry breeding is achieved.

This invention discloses an artificial breeding method for *Schizothorax lancensis*, belonging to the field of aquatic animal breeding technology. The artificial breeding method for *Schizothorax lancensis* provided by this invention includes: intensive three-step cultivation of broodstock, injection of spawning induced agents after cultivation, artificial fertilization, hatching of fertilized eggs, and fry cultivation. Using the artificial breeding method of this invention, the success rate of spawning induction in female *Schizothorax lancensis* is 83.3%, the average fertilization rate is 87.5%, the average hatching rate is 93.0%, and the average fry emergence rate is 91.5%. This achieves large-scale artificial breeding of *Schizothorax lancensis*, laying the foundation for preventing the extinction of *Schizothorax lancensis*, and for carrying out the propagation and release of *Schizothorax lancensis* for industrial utilization.

This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.

The invention discloses an artificial breeding method of lota lota, which comprises the following steps: S1, selecting robust lota lota as parent fish, placing the parent fish in an indoor culture tank for temporary culture, and inducing gonad to develop mature by regulating and controlling water temperature; s2, performing artificial spawning induction on the parent fish with mature gonad to obtain ova and semen, and performing in-vitro fertilization on the ova and the semen to obtain fertilized ova; s3, performing two-step soaking treatment on the fertilized eggs by using a composite biological functional liquid containing a liquid A and a liquid B, and then transferring the fertilized eggs into a hatching facility for hatching to obtain fish fries; and S4, breeding the hatched fish fries. According to the artificial breeding method of the lota lota, the survival rate of fertilized eggs and the survival rate in the fry breeding stage are increased to a high level, large-scale and controllable artificial breeding of the lota lota becomes possible, and the overall technical scheme has high stability and industrial application value.

The invention discloses a breeding method of anti-hypoxia grouper, and belongs to the technical field of grouper breeding. The method comprises the steps of parent fish selection, hypoxia domestication, juvenile fish screening, SNP molecular marker assisted breeding and the like, and finally the hypoxia-resistant grouper variety is obtained. By means of the method, the grouper variety high in hypoxia resistance can be cultivated, the cultivation cost is reduced, and sustainable development of the grouper cultivation industry is promoted.

The invention belongs to the technical field of fish genetic breeding in aquaculture industry, and discloses a grass carp intensive high-yield character genome selective breeding method, which comprises the following steps: establishing a grass carp intensive high-yield character prediction model; the establishment of the grass carp intensive high-yield character prediction model comprises the following steps: (1) obtaining a grass carp candidate parent fish single nucleotide polymorphism site; (2) constructing an analysis model for predicting breeding values of candidate parents; and (3) constructing a molecular genetic relationship matrix by using the obtained single nucleotide polymorphic sites, and predicting a genome estimation breeding value of the candidate group in combination with the analysis model in the step (2). The improved variety breeding method based on grass carp genome information can be used for screening excellent parents suitable for intensive breeding, the screened parents are directly used for breeding offspring seeds, and the high-density breeding tolerance of grass carp can be directly improved; and a technical support is provided for improving cultivation of new grass carp varieties suitable for intensive and facility cultivation.

The invention discloses a pelteobagrus fulvidraco fixing frame and egg squeezing device and a using method, the pelteobagrus fulvidraco fixing frame and egg squeezing device comprises the egg squeezing device and the fixing frame fixedly installed at the top of the egg squeezing device, the egg squeezing device comprises a shell, the fixing frame comprises a supporting plate, the outer wall of the supporting plate is fixedly connected with two hollow plates at equal intervals, and the outer wall of the supporting plate is fixedly connected with the shell. Two arc-shaped elastic pieces are slidably connected into the hollow plate, the outer wall of the hollow plate is an inclined plane, third springs are fixedly connected to the inner wall of the bottom of the hollow plate, the third springs are fixedly connected with the bottoms of the arc-shaped elastic pieces, and the number of the third springs is four; the sliding door is controlled to be closed, after the pelteobagrus fulvidraco touches the pelteobagrus fulvidraco, the parent fish opens, the locked pectoral fin spines are hung on the arc-shaped elastic pieces, the pelteobagrus fulvidraco is conveniently fixed, a motor is started, and fish eggs are conveniently collected while automatic fish egg squeezing work is achieved.

The invention discloses an AI-based trachinotus ovatus parent fish culture water body temperature determination method and system, and the method comprises the steps: collecting culture environment data and parent fish physiological data in real time, and generating a multi-modal time sequence database according to the collected data; based on the multi-modal time sequence database, establishing a target variable data set representing the reproductive performance of the parent fish; an AI prediction model is constructed and trained, a multi-modal time sequence database is used as an input feature, and a target variable data set is used as a prediction target; based on the trained AI prediction model, an optimization target is set, future reproductive performance under different water temperature strategies is simulated within a water temperature preset range, and a water temperature value capable of enabling the optimization target to be optimal is selected as a recommended set temperature according to a simulation result; and sending the recommended set temperature to a temperature control system of the culture pond to execute water temperature regulation and control. According to the method, the temperature of the trachinotus ovatus parent fish culture water body is accurately and intelligently regulated and controlled by fusing the multi-modal time sequence data and the AI prediction model.

The invention provides an artificial propagation method of schizothorax wangchizoides, and belongs to the technical field of fish propagation. The artificial propagation method of the schizothorax wandersoni comprises the following steps that (1) mature parent fish is selected and injected with an oxytocic mixture, and the oxytocic mixture comprises DOM, HCG and LRH-A3; (2) collecting ova and sperms after the parent fish is oestrus, and carrying out artificial insemination; and (3) transferring the fertilized eggs into an incubator for incubation. According to the method, 268345 artificial fertilized eggs are obtained, the fertilization rate reaches 95.6%, 251707 pond fries are obtained after artificial incubation, the hatching rate is 93.8%, and the fertilization rate and the hatching rate of the schizothorax wandersoni are remarkably increased.

The present application relates to a kind of artificial propagation method of Leucoglanis guichenoti.The artificial propagation efficiency of Leucoglanis guichenoti in prior art is low, and the rate of induced spawning, fertilization rate and hatching rate are not high.The propagation method of the present application comprises: using induced spawning agent (water temperature 13-19℃) to Leucoglanis guichenoti parent fish; the parent fish is put into spawning and hatching frame to spawn and fertilize naturally; after taking out the parent fish, keep the water level 5-15cm, and flow water hatching with 2-4 times / day exchange amount to obtain membrane fry; after another 24h culture, flat swimming fry is obtained.The induced spawning agent contains LRH-A2 and DOM, the dosage of female fish is LRH-A2 3-5ug / kg+DOM 5-10mg / kg, the dosage of male fish is LRH-A2 1-3ug / kg+DOM 2-5mg / kg, the effect time of induced spawning agent is 15-19h, the flow water hatching temperature is 17-19℃, and the duration is 42-45h.The method is simple in operation, low in cost, high in induced spawning rate, fertilization rate and hatching rate, can provide technical support for large-scale breeding of Leucoglanis guichenoti, promote the development of aquaculture industry, meet market demand, and has economic and social benefits.

The invention belongs to the technical field of aquaculture, and particularly relates to a decapterus maruadsi breeding feed and application thereof in improving the quality of decapterus maruadsi fertilized eggs. The invention provides a decapterus maruadsi breeding feed. The feed comprises a first feed and a second feed which are mutually independent, the first feed comprises a basal feed, fish cream and a nutrition enhancer; the second feed comprises the first feed and oyster meat; the nutrition enhancer contains calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin C and vitamin F. When the culture feed for the decapterus maruadsi is used for performing nutrient enrichment on parent fishes of the decapterus maruadsi, synchronous development of sexual glands of male and female parent fishes of the decapterus maruadsi can be promoted, the quality of fertilized eggs of the decapterus maruadsi is improved, the good egg rate of the fertilized eggs of the decapterus maruadsi reaches 88.50% and is 39.15% higher than that of a control group, and the fertilization rate of the fertilized eggs reaches 79.58% and is 19.46% higher than that of the control group.

The present application relates to the field of domestication of fish, and particularly relates to an artificial domestication method of Leptobotia rubipinnis. The method comprises the following steps: collecting and transporting wild parent fish, temporarily raising the wild parent fish, and domesticating the wild parent fish. The domestication process of the wild parent fish comprises the following steps: ① feeding biological bait, ② feeding biological bait and artificial compound feed, and ③ feeding artificial compound feed. The method has the advantages of simple operation process, and the use of the segmented temporary raising and domestication, gradual conversion from biological bait to artificial compound feed, and regulation of water flow rate during feeding, so that the death of the parent fish due to starvation is avoided, thereby improving the survival rate (more than 75%), the food conversion rate and the artificial domestication survival rate (≥68%) of the wild parent fish, and providing technical support and basic data accumulation for the artificial propagation, disease prevention and control and artificial propagation and release of Leptobotia rubipinnis.

The application provides an artificial breeding method of Onychostoma wujiangense, and belongs to the technical field of aquatic animal breeding. The artificial breeding method of Onychostoma wujiangense comprises the steps of parent fish classification, parent fish cultivation, artificial parturition induction, natural insemination, artificial insemination, fertilized egg hatching, larva cultivation and juvenile fish cultivation. The parturition induction agent is reasonably matched, the parturition induction injection time is accurate, the parturition induction rate, the fertilization rate and the hatching rate are improved, and the parent fish damage is small. The application also clearly defines the feeding methods at different stages of seed cultivation, improves the survival rate of Onychostoma wujiangense seed cultivation stage, and lays a technical support for the artificial breeding of the endangered species Onychostoma wujiangense.

The invention provides a sea bass breeding method which comprises the following steps: parent fish selection and intensive cultivation: selecting 3-5-year-old healthy parent fish, domesticating in a circulating water system with illumination, water temperature and salinity, and feeding intensive feed to promote gonad development; step-by-step spawning induction: injecting the female fish twice, and injecting the male fish once to improve spawning induction synchronism; the semen and the eggs are mixed and activated through ultraviolet irradiation, and the fertilization efficiency is improved; pulse-type water flow hatching: inhibiting water mildew by combining with sound wave oscillation, wherein the hatching rate reaches 85% or above; staged fry breeding: phage-probiotic enhanced rotifers are fed in the fry stage, and microcapsule feed coated with sodium alginate-chitosan is used in the juvenile stage, so that the survival rate and the growth speed of the fry are remarkably increased. The method solves the problems of low fertilization rate, weak seedling disease resistance and the like in traditional breeding through methods of environment simulation, nutrient enrichment, biological prevention and control and the like, and is suitable for large-scale seedling breeding and deep-sea breeding.

The application discloses a method for obtaining high-quality fertilized eggs of wild Pseudosciaena crocea of Lutjanus, and belongs to the technical field of aquatic animal breeding. Wild parent fishes are captured in the breeding season of Pseudosciaena crocea of Lutjanus and are transported to a breeding farm; the wild parent fishes are temporarily raised and intensively cultured, and male and female wild parent fishes with mature gonads are selected, and artificial parturition mixed hormones are injected after anesthesia; after the injection of the artificial parturition mixed hormones, ova and sperm are collected, artificial insemination is performed, and high-quality fertilized eggs are obtained. The technical steps in the application are correlated with each other to form a whole, high-quality fertilized eggs of wild Pseudosciaena crocea of Lutjanus can be successfully obtained, and the proportion of the high-quality fertilized eggs is more than 45%. The high-quality fertilized eggs obtained by the application are used for fry hatching, the hatching rate of the fertilized eggs is more than 62%, and the high-quality fertilized eggs can be used for constructing a basic population of wild Pseudosciaena crocea of Lutjanus, so that artificial breeding of the wild Pseudosciaena crocea of Lutjanus is realized.