104 results about "Broodstock" patented technology

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention provides a polypeptide with an ovary maturation promoting effect and application of the polypeptide in parturition induction of pomfret. The amino acid sequence of the polypeptide is SEQ ID NO: 1. The polypeptide provided by the invention is derived from the GnRH2 gene of the pomfret, and in-vivo experiments prove that the polypeptide can efficiently stimulate the pituitary of the pomfret to secrete gonadotropin, so that the spawning induction time is remarkably shortened, and the spawning rate and the relative spawning amount of parent fish are increased. The polypeptide is strong in species specificity and good in physiological compatibility, does not generate an antibody, avoids side effects of traditional heterologous hormones, is safe and effective, and has a huge potential of being developed into a special spawning induction agent for the pomfret.

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

This invention belongs to the field of aquaculture equipment and discloses a recirculating aquaculture system for indoor multi-layered culture of freshwater fish germplasm samples. The system includes a stainless steel culture rack, glass culture tanks, a temperature control system, a recirculating water purification system, an aeration system, and electrical and auxiliary materials. Through a three-layered culture structure design, combined with culture tanks of varying sizes and a precise temperature control module, it solves the problem of aquaculture with diverse sizes of freshwater fish germplasm samples and large differences in suitable water temperatures. The system has nine small glass culture tanks in the upper layer and three large glass culture tanks in each of the middle and lower layers, equipped with an external temperature control component and an internal heating rod, achieving full-coverage water temperature regulation from 10℃ to above 30℃. The recirculating water system ensures stable water quality through the synergistic effect of a filter tank, a booster pump, an ultraviolet sterilizer, and various filter media. Meanwhile, the lower culture tanks are equipped with an internal circulation filter to enhance local water purification, meeting the diverse aquaculture needs from small fry to large broodstock.

The present invention relates to the technical field of aquaculture and breeding of seawater fishes, in particular to an industrialized cultivation method for cephalopholis sonnerati fry, comprising the steps of successively subjecting parent fishes of cephalopholis sonnerati to feed domestication and nutrient enrichment at a water temperature of 25-29°C and under a flowing water condition, so as to obtain parent fishes with fully developed gonads, etc. The present invention takes strict measures for fry quality control and infectious disease prevention and control during industrialized workshop cultivation, so as to effectively optimize the growth conditions of fry, thus increasing the growth rate and improves the health conditions and adaptability of fry while reducing the risk of diseases. By means of standardized breeding management and technical means, the growth environment and feeding management of fry can be controlled, thus effectively improving production efficiency. The cultivated cephalopholis sonnerati has the advantages of fast growth rate, high disease resistance, strong vigor, tender and delicious flesh and good market prospects.

The invention discloses a squaliobarbus curriculus breeding and breeding method based on aquaculture. According to the method, slow flow domestication is started from the fry stage, the fries are made to adapt to the dynamic water environment from small, the physique is obviously enhanced, the fries are not prone to being infected with diseases during adult fish culture, and the trouble and cost of medication are reduced. In addition, through the feed transition domestication in the S3, the palatability of the fries eating the compound feed in the later stage is better, the growth speed is more uniform, the subsequently screened water-infused fishes are neat in specification, the water-infused fishes can more quickly adapt to fasting and water flow environments during water infusing, the weight-reducing and fishy smell-removing effects are more stable, the meat quality is compact and elastic, the mud fishy smell basically disappears, and the quality and the appearance of the fishes are directly improved; a cycle is formed from parent fish selective culture to sales feedback, the parent fish breeding direction and the adult fish culture density can be adjusted in time according to the requirements of the market end, the unsalable risk caused by blind culture is avoided, and the income of farmers is guaranteed.

This invention discloses an artificial breeding method for *Schizothorax lancensis*, belonging to the field of aquatic animal breeding technology. The artificial breeding method for *Schizothorax lancensis* provided by this invention includes: intensive three-step cultivation of broodstock, injection of spawning induced agents after cultivation, artificial fertilization, hatching of fertilized eggs, and fry cultivation. Using the artificial breeding method of this invention, the success rate of spawning induction in female *Schizothorax lancensis* is 83.3%, the average fertilization rate is 87.5%, the average hatching rate is 93.0%, and the average fry emergence rate is 91.5%. This achieves large-scale artificial breeding of *Schizothorax lancensis*, laying the foundation for preventing the extinction of *Schizothorax lancensis*, and for carrying out the propagation and release of *Schizothorax lancensis* for industrial utilization.

This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.

The invention discloses an artificial breeding method of lota lota, which comprises the following steps: S1, selecting robust lota lota as parent fish, placing the parent fish in an indoor culture tank for temporary culture, and inducing gonad to develop mature by regulating and controlling water temperature; s2, performing artificial spawning induction on the parent fish with mature gonad to obtain ova and semen, and performing in-vitro fertilization on the ova and the semen to obtain fertilized ova; s3, performing two-step soaking treatment on the fertilized eggs by using a composite biological functional liquid containing a liquid A and a liquid B, and then transferring the fertilized eggs into a hatching facility for hatching to obtain fish fries; and S4, breeding the hatched fish fries. According to the artificial breeding method of the lota lota, the survival rate of fertilized eggs and the survival rate in the fry breeding stage are increased to a high level, large-scale and controllable artificial breeding of the lota lota becomes possible, and the overall technical scheme has high stability and industrial application value.

The invention discloses an AI-based trachinotus ovatus parent fish culture water body temperature determination method and system, and the method comprises the steps: collecting culture environment data and parent fish physiological data in real time, and generating a multi-modal time sequence database according to the collected data; based on the multi-modal time sequence database, establishing a target variable data set representing the reproductive performance of the parent fish; an AI prediction model is constructed and trained, a multi-modal time sequence database is used as an input feature, and a target variable data set is used as a prediction target; based on the trained AI prediction model, an optimization target is set, future reproductive performance under different water temperature strategies is simulated within a water temperature preset range, and a water temperature value capable of enabling the optimization target to be optimal is selected as a recommended set temperature according to a simulation result; and sending the recommended set temperature to a temperature control system of the culture pond to execute water temperature regulation and control. According to the method, the temperature of the trachinotus ovatus parent fish culture water body is accurately and intelligently regulated and controlled by fusing the multi-modal time sequence data and the AI prediction model.

The invention provides an artificial propagation method of schizothorax wangchizoides, and belongs to the technical field of fish propagation. The artificial propagation method of the schizothorax wandersoni comprises the following steps that (1) mature parent fish is selected and injected with an oxytocic mixture, and the oxytocic mixture comprises DOM, HCG and LRH-A3; (2) collecting ova and sperms after the parent fish is oestrus, and carrying out artificial insemination; and (3) transferring the fertilized eggs into an incubator for incubation. According to the method, 268345 artificial fertilized eggs are obtained, the fertilization rate reaches 95.6%, 251707 pond fries are obtained after artificial incubation, the hatching rate is 93.8%, and the fertilization rate and the hatching rate of the schizothorax wandersoni are remarkably increased.

The present application relates to a kind of artificial propagation method of Leucoglanis guichenoti.The artificial propagation efficiency of Leucoglanis guichenoti in prior art is low, and the rate of induced spawning, fertilization rate and hatching rate are not high.The propagation method of the present application comprises: using induced spawning agent (water temperature 13-19℃) to Leucoglanis guichenoti parent fish; the parent fish is put into spawning and hatching frame to spawn and fertilize naturally; after taking out the parent fish, keep the water level 5-15cm, and flow water hatching with 2-4 times / day exchange amount to obtain membrane fry; after another 24h culture, flat swimming fry is obtained.The induced spawning agent contains LRH-A2 and DOM, the dosage of female fish is LRH-A2 3-5ug / kg+DOM 5-10mg / kg, the dosage of male fish is LRH-A2 1-3ug / kg+DOM 2-5mg / kg, the effect time of induced spawning agent is 15-19h, the flow water hatching temperature is 17-19℃, and the duration is 42-45h.The method is simple in operation, low in cost, high in induced spawning rate, fertilization rate and hatching rate, can provide technical support for large-scale breeding of Leucoglanis guichenoti, promote the development of aquaculture industry, meet market demand, and has economic and social benefits.

The invention belongs to the technical field of aquaculture, and particularly relates to a decapterus maruadsi breeding feed and application thereof in improving the quality of decapterus maruadsi fertilized eggs. The invention provides a decapterus maruadsi breeding feed. The feed comprises a first feed and a second feed which are mutually independent, the first feed comprises a basal feed, fish cream and a nutrition enhancer; the second feed comprises the first feed and oyster meat; the nutrition enhancer contains calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin C and vitamin F. When the culture feed for the decapterus maruadsi is used for performing nutrient enrichment on parent fishes of the decapterus maruadsi, synchronous development of sexual glands of male and female parent fishes of the decapterus maruadsi can be promoted, the quality of fertilized eggs of the decapterus maruadsi is improved, the good egg rate of the fertilized eggs of the decapterus maruadsi reaches 88.50% and is 39.15% higher than that of a control group, and the fertilization rate of the fertilized eggs reaches 79.58% and is 19.46% higher than that of the control group.

The present application relates to the field of domestication of fish, and particularly relates to an artificial domestication method of Leptobotia rubipinnis. The method comprises the following steps: collecting and transporting wild parent fish, temporarily raising the wild parent fish, and domesticating the wild parent fish. The domestication process of the wild parent fish comprises the following steps: ① feeding biological bait, ② feeding biological bait and artificial compound feed, and ③ feeding artificial compound feed. The method has the advantages of simple operation process, and the use of the segmented temporary raising and domestication, gradual conversion from biological bait to artificial compound feed, and regulation of water flow rate during feeding, so that the death of the parent fish due to starvation is avoided, thereby improving the survival rate (more than 75%), the food conversion rate and the artificial domestication survival rate (≥68%) of the wild parent fish, and providing technical support and basic data accumulation for the artificial propagation, disease prevention and control and artificial propagation and release of Leptobotia rubipinnis.

The application provides an artificial breeding method of Onychostoma wujiangense, and belongs to the technical field of aquatic animal breeding. The artificial breeding method of Onychostoma wujiangense comprises the steps of parent fish classification, parent fish cultivation, artificial parturition induction, natural insemination, artificial insemination, fertilized egg hatching, larva cultivation and juvenile fish cultivation. The parturition induction agent is reasonably matched, the parturition induction injection time is accurate, the parturition induction rate, the fertilization rate and the hatching rate are improved, and the parent fish damage is small. The application also clearly defines the feeding methods at different stages of seed cultivation, improves the survival rate of Onychostoma wujiangense seed cultivation stage, and lays a technical support for the artificial breeding of the endangered species Onychostoma wujiangense.

This invention discloses a method for evaluating the effectiveness of stock enhancement and release based on microsatellite markers. The method involves collecting a dataset of recaptured samples of released species; obtaining microsatellite genetic data of released broodstock, released fry, and recaptured samples; performing consistency processing on the microsatellite genetic data of released broodstock to supplement the virtual parent microsatellite genotype database; generating an allele contribution probability weight matrix to obtain an allele contribution probability dataset of recaptured samples; generating a basic matching list to construct a contribution weight dataset; and outputting the stock enhancement and release contribution rate evaluation results and storing them in a stock enhancement and release evaluation database. This invention makes the stock enhancement contribution rate no longer simply dependent on the number of matches, but comprehensively reflects the true contribution of recaptured individuals in the overall genetic background, thus improving the accuracy and robustness of stock enhancement and release effectiveness evaluation from an algorithmic perspective.

The technical scheme of the utility model discloses a continuous automatic injection device for hastening parturition of masuhua parent fish. The continuous automatic injection device comprises a movable bracket, the fixing device is movably connected to the movable support and used for fixing the parent fish; the injection device is connected to the movable support, located beside the fixing device and used for injecting the parent fish fixed by the fixing device; wherein the injection device is provided with a peristaltic pump, the peristaltic pump is connected with the controller and used for controlling the dosage of injection every time, and automatic injection of the oxytocic agent is achieved through the peristaltic pump. The automatic injection device has the advantages of being easy and convenient to operate, accurate in dosage, high in efficiency, safe and reliable, automatic injection can be achieved, parent fishes do not need to be caught repeatedly, and the stress response of the parent fishes is reduced. And accurate injection dosage can be ensured, and the spawning induction effect is improved. Multiple parent fishes can be continuously injected, so that the spawning induction efficiency is greatly improved, and the large-scale production requirement is met. Meanwhile, the injection process is safe and reliable, and fish bodies are prevented from being damaged.

The invention discloses a recirculating water breeding system for simulating natural ocean current and a production promoting method, and belongs to the technical field of aquaculture engineering. The invention discloses a circulating water breeding system and a production promoting method for simulating natural ocean current. The system comprises a culture pond body structure, a bionic water flow stimulation subsystem, a circulating water treatment subsystem and an intelligent control subsystem, the method comprises the following steps of: preparing the system in a pond entering stage of parent fish of the Dongquasichthys nobilis, a gonad development promoting stage, an oviposition inducing stage and a postpartum management stage. The problems that in the prior art, a water flow mode is single and fixed, a gonad development stimulation mode is extensive, artificial insemination is relied on, the automation degree is low, and the overall breeding efficiency is low are solved. According to the method, an integrated circulating water culture environment is constructed, and complex tide and ocean current conditions of a natural spawning site of the Dongcao are accurately simulated, so that gonad development synchronization of parent fishes is effectively stimulated, the natural breeding behavior of the parent fishes is triggered, and the spawning amount and the fertilization rate are remarkably increased.

Provided is a culturing facility comprising: a rearing water tank for rearing a cultured fish stock; an inspection device for performing a non-destructive inspection of a living cultured fish stock; and an information processing device that derives information relating to the amount of at least one organ on the basis of an inspection result acquired by the inspection device. Provided is a method for culturing fishes, comprising a selection step for selecting a parent fish from a cultured fish stock on the basis of information relating to the amount of at least one organ acquired during a non-destructive inspection of a living cultured fish stock.

The invention discloses an out-of-season artificial propagation technical method for crucian carp. The technical method specifically comprises the key steps of water temperature and illumination control of an intelligent culture system, parent fish screening and concentrated intensified culture, parent fish ripening acceleration, artificial spawning induction, artificial insemination, incubation and the like. Aiming at seasonal limitation of natural breeding of the crucian carp, by scientifically regulating and controlling an illumination period and a water temperature environment and accurately simulating ecological conditions suitable for breeding of the crucian carp, it is ensured that parent fishes of the crucian carp can synchronously reach physiological states of periodic normal ovulation and spermiation in an unnatural spawning season from October to January of the next year; the activity and quality of germ cells can be obviously improved; meanwhile, an innovative breeding mode and an exclusive feeding scheme are combined, the industrial pain points that eggs of female crucian carp of ice cream are prone to aging and parent fishes only spawn once a year in a natural state are effectively overcome, and anti-season stable breeding is achieved.

This invention provides a method for the artificial breeding of Litopenaeus vannamei, including steps such as scientifically selecting broodstock, optimizing the breeding environment, controlling the breeding process, fertilization, hatching of fertilized eggs, and intensive rearing. During the breeding process, specific photoperiods and staged feeding strategies are employed, with different ratios of formulated feed and nutritional preparations to meet the nutritional needs of the broodstock. After initial rearing, the hatched juvenile shrimp are graded and selected, followed by intensive rearing. During the intensive rearing stage, water quality parameters are adjusted, and necessary trace elements, vitamins, and specific feeds are administered to promote the healthy growth of the juvenile shrimp. This invention, through meticulous management, significantly improves the breeding success rate and larval survival rate of Litopenaeus vannamei, providing an effective breeding strategy for aquaculture.

The invention relates to the technical field of cultivation, and discloses a gymnocyprinus carpio cultivation device which comprises a cultivation pool body, a plurality of sets of programmable water power assemblies are arranged in the cultivation pool body and communicate with a circulating water assembly located outside the cultivation pool body, and the circulating water assembly is communicated with the programmable water power assemblies. The outer wall of the water inlet of the cultivation pool body is sleeved with a non-thermal physical disinfection module. Through the programmable water power assembly, free switching of various flow states such as bottom layer washing laminar flow, whole pool suspension micro-circulation flow and pulse stimulation flow can be achieved in one cultivation pool body. In the spawning period, strong jet flow simulates the scouring environment of a natural riverbed, and the parent fish is effectively stimulated to ovulate; in the mouth opening period, special micro-circulation enables mouth opening bait to suspend through fluid lift force, and the problems that in traditional breeding, bait sinks to the bottom, and juvenile fishes are starved to death are solved; in the ripening accelerating period, the pulse water flow provides necessary movement load.

The invention discloses a net cage for fish culture and breeding, and relates to the technical field of net cages for fish culture and breeding, the net cage comprises an outer floating ring and an inner floating ring, and further comprises a connecting frame used for connecting the outer floating ring and the inner floating ring, and a V-shaped fixing arm is installed below the connecting frame; the V-shaped structural bodies are connected forwards and backwards to form an X-shaped structural body, one V-shaped structural body is connected with the V-shaped fixed arm, and the multiple V-shaped structural bodies are connected in series in the linear direction; the continuous supporting frame in the depth direction is constructed, the fishing nets are arranged on the inner side and the outer side of the V-shaped structural body respectively, an all-dimensional stress system is formed in a three-dimensional space, the fishing nets are continuously supported, the shape stability of the fishing nets is effectively maintained, the twisting phenomenon caused by water flow impact is avoided, it is ensured that the internal space is sufficient and uniform, and the service life of the fishing net is prolonged. Therefore, the cultured fishes and the bred parent fishes are prevented from being extruded.

A fish egg hatching device includes a barrel containing a detachable fixing component. A broodstock net box is mounted on the fixing component, with openings at both the top and bottom. The lower end of the broodstock net box connects to a hatching net box. A separation component allows fish eggs to pass through between the broodstock and hatching net boxes. One side of the hatching net box has an openable door, and the other side has a barrier component allowing fry to pass through. An aeration component is located at the bottom of the barrel. One end of the barrel has a water inlet pipe, and the other end has a drain pipe containing a filter screen. This invention employs a double-layer structure of broodstock and hatching net boxes, ensuring sufficient space for broodstock activity and a suitable hatching environment for the fish eggs. The separation component separates the broodstock and eggs, preventing fry from swimming upstream and effectively preventing broodstock from consuming the eggs. It also prevents egg loss and avoids egg accumulation, thus improving the hatching survival rate.

This invention discloses an artificial breeding method for the Oriental blackfish, belonging to the field of fish artificial breeding technology. It includes six core steps: broodstock selection and domestication, enhanced maturation cultivation, broodstock selection and anesthesia, spawning induction, artificial insemination, fertilized egg hatching, and fry rearing. One year in advance, suitable-aged wild Oriental blackfish are selected and domesticated in a flowing water pond. Before breeding, tocopherol is mixed into their feed to enhance gonadal development. High-quality broodstock are selected at a female-to-male ratio of 1:2 and anesthetized. A specific ratio of spawning inducing agent is injected using a two-injection method. Artificial insemination is performed in a standardized fiberglass circular fishpond under controlled water flow conditions. Fertilized eggs are incubated at 26±1℃ for 22 hours, and after hatching, they are transferred to a sterilized micro-flow water pond for rearing. This invention achieves a 100% spawning efficiency, a stable fertilization rate of over 95%, and a hatching rate exceeding 82%. It is simple to operate, cost-effective, and allows for large-scale breeding, providing key technical support for the conservation and population expansion of the Oriental blackfish.

The application provides a method for cultivating all-male hybrid yellow-head catfish by using double super-male population hybridization, comprising the following steps: S1, constructing super-male maintenance lines of yellow-head catfish and Pelteobagrus vishnui by using sex reversal technology and gender molecular markers; S2, performing artificial propagation on the maintenance lines to expand the super-male fish in batches; S3, using androgen antagonists to partially replace estrogen for the expanded super-male fish to cooperatively induce super-female fish; S4, applying specific nutrient enrichment culture to the super-female fish and super-male fish parents; and S5, performing positive and negative cross artificial propagation between the super-female fish and super-male fish of the two super-male populations to obtain a heterogamy super-male population, i.e., commercial fry of all-male hybrid yellow-head catfish. The technical scheme of the application can efficiently and in batches induce super-female fish, realize high-density culture of parent fish, and significantly improve the effect and efficiency of artificial propagation; the hybrid all-male yellow-head catfish has high uniformity, a growth speed of 20% and a male rate of 100%.

The present application relates to grouper aquaculture technical field, specifically to a kind of pseudotolithus elongatus wild-like breeding device and method, including frame system and netting system installed on frame system, it is characterized in that, frame system includes platform, the circumferential of platform is equipped with several movable walking frame, walking frame is all connected with the arc-shaped float pipe group, air bag is installed between adjacent float pipe group, air bag is communicated with float barrel, and float barrel is installed at the side of float pipe group far from walking frame;The bionics tube for simulating the wild environment of pseudotolithus elongatus is installed below walking frame;Netting system includes netting and bionic plate installed at the bottom of netting.The present application can improve the survival rate of pseudotolithus elongatus, improve product fish quality, and also can be used for broodstock cultivation.

The invention relates to a three-way hybrid grouper new germplasm cultivation method, and belongs to the technical field of grouper genetic breeding. According to the method, epinephelus moara and golden tiger hybridized fries (female epinephelus fuscoguttatus * male epinephelus coioides) are used as breeding materials, three-way hybridization is carried out in a matching mode of female epinephelus moara * male epinephelus coioides, and breeding of three epinephelus genetic species and fry with excellent characteristics is achieved. The method comprises the steps of parent selection, parent fish reproduction regulation and control, parent fish spawning induction, gamete collection, artificial insemination, incubation and fry rearing. According to the method, on the basis of reproductive regulation and crossbreeding technologies, time and space reproductive isolation of the groupers is broken through, and the new grouper germplasm which is high in survival rate, low in malformation rate and high in growth speed is cultivated.