304 results about "Broodstock" patented technology

The invention provides a method for calculating migratory parent fish quantity by using fish early-stage resource quantity, which comprises the following steps: collecting early-stage resource quantity data, migratory parent fish quantity data and various parameters, and processing the collected data to obtain a comprehensive data set; constructing a basic model for representing the relationship among the number of roes, the number of larva fishes and the number of migratory parent fishes in combination with various parameters; designing a model architecture of the basic model by using a long-short-term memory network to obtain an initial prediction model, and quantifying the initial prediction model by using a Bayesian method to obtain a calculation prediction model; in combination with an MCMC method, performing parameter estimation and model training on the reckoning prediction model to realize optimization of the reckoning prediction model; and calculating the number of migratory parent fishes by using the optimized calculation and prediction model. According to the method, multiple data sources can be combined, the ecological environment complexity, the time sequence dynamics and the prediction uncertainty are fully considered, and accurate calculation of the migratory parent fish quantity is achieved.

The invention provides an ecology-imitating artificial propagation method for Dongquasichthys nobilis. The ecology-imitating artificial propagation method comprises the following steps: S1, selecting male and female parent fishes and putting the male and female parent fishes into an artificial ecology-imitating breeding pond for breeding; s2, in a non-breeding season, feeding female parent fish feed A for 2-3 times every day, and feeding male parent fish feed B for 2-3 times every day; s3, 2-3 months before breeding, female parent fish fortified feed I is fed 2-3 times every day, and male parent fish fortified feed II is fed 2-3 times every day; s4, after the gonad of the parent fish is mature, artificial spawning induction is conducted, and fertilized eggs are collected and hatched. According to the method, the ecology-imitating breeding environment is manufactured, so that the east astrostroma parent fish adapts to the artificial environment, the irritability is reduced, the east astrostroma parent fish is fed with different feeds in the non-breeding season and the breeding season, the ecological factor and nutritional requirements for gonad development of the east astrostroma can be met, the immunity of the east astrostroma parent fish is improved, gonad maturation is promoted, and the survival rate of the east astrostroma parent fish is increased. The quality of sperms and ova is improved, the fertilization probability is improved, the malformation probability of hatched larvae is reduced, and the breeding efficiency is improved.

The present disclosure relates to the technical field of marine fish cultivation and breeding, and in particular to an industrialized cultivation method for a cephalopholissonnerati fry. The method includes: a step of sequentially performing food acclimation and nutrient enrichment on a cephalopholissonnerati parent fish under conditions of 25-29° C. of water temperature and running water, to obtain the parent fish with mature gonad development, and other steps.

The invention discloses a method for obtaining high-quality fertilized eggs of wild sal-family pseudosciaena crocea, and belongs to the technical field of aquatic animal breeding. The method comprises the following steps: capturing wild parent fishes in the breeding season of the sal-family pseudosciaena crocea and transporting the wild parent fishes to a farm; the method comprises the following steps: carrying out temporary culture and intensive culture on wild parent fishes, selecting male and female wild parent fishes with mature gonads, anesthetizing, and injecting artificial spawning induction mixed hormones; after artificial spawning mixed hormones are injected, ova and seminal fluid are collected, and high-quality fertilized ova are obtained through artificial insemination. According to the method, all the technical steps are associated with one another to form a whole, the high-quality fertilized eggs of the large yellow croakers of the wild sal ammoniac family can be successfully obtained, and the proportion of the obtained high-quality fertilized eggs reaches 45% or above. According to the method, the obtained high-quality fertilized eggs are adopted for fry hatching, the hatching rate of the obtained fertilized eggs reaches 62% or above, the fertilized eggs can be used for constructing and breeding a wild sal-family pseudosciaena crocea basic population, and artificial breeding of the sal-family pseudosciaena crocea is achieved.

The invention provides an artificial breeding method of black head fish, and belongs to the technical field of aquaculture. The spawning induction method comprises the following steps: injecting spawning induction hormone into a female parent for breeding parent fish twice for artificial spawning induction to obtain fertilized eggs; during the incubation period of the fertilized eggs, micro-flow at 18-21 DEG C is adopted for artificial incubation, and the flow speed is controlled by water at different incubation stages until incubation and seedling emergence are achieved, so that the fry of the black head fish with the odd amount is obtained. According to the method, the oxytocic hormone is injected in a two-needle injection mode, the injection dosage of each needle is strictly controlled, ova and seminal fluid which are well developed can be obtained, meanwhile, the hatching conditions of the fertilized ova are controlled, the oxytocic rate of the black head fish is 100%, the fertilization rate is 89% or above, the hatching rate is 86% or above, large-scale artificial breeding of the black head fish is achieved, and the method is suitable for large-scale artificial breeding of the black head fish. The method has important significance for protecting the cyprinus carpio which is an endangered resource.

The invention discloses a method for targeted creation of a fast-growing new tilapia strain, and belongs to the technical field of fish cultivation. The method specifically comprises the following steps: (1) taking a fragment, as shown in SEQ ID NO.1, on a third exon of a pomc gene as a gRNA target sequence; (2) designing and synthesizing a gRNA primer sequence; (3) amplifying by adopting the gRNA primer sequence and the gRNA plasmid, and then transcribing and purifying to obtain a gRNA fragment; (4) transferring the gRNA fragments into fertilized eggs of tilapia mossambica through gene editing to obtain F0-generation positive fish; and (5) hybridizing the F0-generation positive male fish with the wild-type female fish, screening out F1-generation parent fish, and culturing the F1-generation parent fish to obtain F2-generation tilapia with homozygous deletion mutation. According to the invention, the pomc homozygous knockout line is established in the Nile tilapia mossambica for the first time, the new fast-growing line is a complete gene function deletion model, the cost is lower, the efficiency is higher, and the phenotype can be stably inherited.

The invention provides a breeding method of golden and tiger hybrid spotted fish eggs. The breeding method comprises the following steps: S1, parent screening; s2, parent cultivation; s3, artificial insemination: (1) spawning induction: when the belly of the female parent fish expands, the ovary develops to the IV stage and the male parent fish presses the belly to cause white seminal fluid to flow out, performing oxygenation flowing water stimulation on the female parent fish, and performing irradiation with a TDP radiation lamp for spawning induction; (2) fertilization; s4, artificial incubation: (1) arranging an incubator, injecting seawater, and performing plasma treatment to obtain a treated incubator; and (2) adding the fertilized eggs into the treated incubator, carrying out magnetization treatment, adding chitosan, and hatching to obtain the larva fish. According to the breeding method, the sperm and egg quality can be improved, the fertilization rate and the hatching rate are remarkably increased, and according to verification of the embodiment, the fertilization rate ranges from 97.4% to 99.3%, and the hatching rate ranges from 95.6% to 97.1%. The hatched larvae are not deformed, the survival rate is 96%-99%, the quality of the larvae is high, and a good foundation is established for obtaining high-quality hybrid Jinhuasu.

A macrobrachium rosenbergii breeding method belongs to the technical field of shrimp breeding, and comprises the following steps: selection of parent shrimps: selecting a macrobrachium rosenbergii pseudo-female population introduced from Thailand as female shrimps, and stocking in a breeding pond A; screening domestic male groups which are robust in physique and are not carried by viruses, and stocking the male groups in the breeding pond B; thailand pseudo female shrimps and domestic male shrimps are subjected to cross breeding, and the domestic male shrimps (South Taihu No.3) are subjected to cross breeding with the Thailand pseudo female shrimps by virtue of relatively high low temperature resistance, so that the low temperature resistance of hybrid offspring is improved, and the current breeding situation of Thailand Macrobrachium macrobrachium offspring seeds in China is improved.

The present disclosure relates to the technical field of marine fish cultivation and breeding, and in particular to an industrialized cultivation method for a Cephalopholissonnerati fry. The method includes: a step of sequentially performing food acclimation and nutrient enrichment on a Cephalopholissonnerati parent fish under conditions of 25-29° C. of water temperature and running water, to obtain the parent fish with mature gonad development, and other steps.

The invention provides a method for cultivating hybrid fries of Jinhu and tiger. The method comprises the following steps: selecting a breeding environment; breeding and ripening female parents; management and control before oviposition; insemination; hatching fertilized eggs; fry rearing. According to the method, in the female parent breeding and ripening stage, specific biological bait is controlled to be fed in stages, feeding of the parent fish can be promoted, growth can be accelerated, in cooperation with specific compound feed in the management and control stage before oviposition, favorable conditions are created for subsequent oviposition, and the quality and number of ova can be improved. Low-frequency ultrasonic assisted fertilization is introduced in the artificial insemination stage, so that the activity of ovum sperms can be obviously improved, and the fertilization rate is increased; in the fertilized egg hatching stage, intermittent ultrasound is assisted, the hatching rate can be increased, and the seedling yield of the golden-tiger hybrid spots can be increased; after hatching, transferring into a specific culture pond, slowly releasing beneficial bacteria, improving the adaptability of the fry, adsorbing harmful substances, reducing pathogenic bacteria, stabilizing water quality, promoting the growth of the fry, improving the survival rate and reducing the malformation rate.

The invention provides a low-temperature-resistant trachinotus ovatus fry breeding method which comprises the following steps: (1) parent selection and breeding: taking trachinotus ovatus as a female parent, and carrying out intensive culture before insemination; then low-temperature stress culture is carried out under the condition of 18-20 DEG C, and the survival and normal ingestion parent fishes are used for the subsequent breeding step; (2) cross breeding: screening out sexually mature parents for induced spawning to complete artificial insemination; when the larvae are hatched out of membranes, the larvae are transferred into a breeding pond for fry breeding; (3) feeding rotifer and copepod larvae to the newly hatched larvae; when the larvae are transformed into juvenile fishes, feed containing drosera burmannii is fed, and when the juvenile fishes are transformed into juvenile fishes, feed containing eucommia ulmoides vines is fed for at least 15 days. According to the method, the low-temperature resistance of the trachinotus ovatus fries is effectively improved, and the method is easy to operate, low in cost and easy to apply and popularize.

The utility model belongs to the technical field of fish culture, and particularly relates to an acrossocheilus fasciatus roe collecting and hatching integrated device. Comprising a water storage frame used for providing a water environment, an egg collecting frame used for collecting fish eggs and an egg laying frame used for parent egg laying, frame bodies of the egg laying frame, the egg collecting frame and the water storage frame are gradually enlarged, and the egg laying frame, the egg collecting frame and the water storage frame are sequentially nested and erected from top to bottom, so that the frame bodies form three layers of nested structures from inside to outside; an ovipositor is arranged in the oviposition frame and is provided with a gravel-imitating rough surface, the bottom of the oviposition frame is provided with a first hole allowing roes to pass through, and the bottom of the roe collecting frame is provided with a second hole allowing water to pass through. The device is simple in structure and convenient to use, parent fish and fertilized eggs can be conveniently separated, after the fertilized eggs are hatched, fish fries can be conveniently transferred, the hatching rate is increased, and the survival amount of offspring is increased.

The invention provides an artificial breeding method of wild sal-family large yellow croaker, and belongs to the technical field of aquatic animal breeding. The invention provides an artificial breeding method of wild sal-family pseudosciaena crocea, which comprises the following steps: domesticating the wild sal-family pseudosciaena crocea, then carrying out intensive cultivation, and after the wild sal-family pseudosciaena crocea is cultivated until gonad is mature, carrying out artificial spawning induction and natural fertilization on the obtained parent fish of the sal-family pseudosciaena crocea to obtain fertilized eggs; the fertilized eggs are subjected to incubation and fry cultivation, and sal-family large yellow croaker fries are obtained; the artificial spawning method comprises the step of injecting hormone into parent fish in two steps. According to the method, favorable conditions are created for large-scale breeding of the sal-family pseudosciaena crocea, and a large number of artificially bred sal-family pseudosciaena crocea fry groups can be obtained by adopting the method.

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention provides a method for breeding all-male hybrid pelteobagrus fulvidraco through hybridization of double super-male populations, which comprises the following steps: S1, respectively constructing super-male maintenance lines of pelteobagrus fulvidraco and pelteobagrus vachelli by utilizing a sex reversal technology and a sex molecular marker; s2, carrying out artificial propagation on the maintenance line, and propagating the super male fish in batches; s3, partially replacing estrogen with an androgen antagonist for the propagated supermale fish, and synergistically inducing the supermale fish to obtain superfemale fish; s4, performing specific nutrient enrichment cultivation on the super female fish and the super male parent fish; and S5, carrying out reciprocal cross artificial propagation on the super-female fish and the super-male fish between the two super-male groups to obtain a heterozygous super-male group, namely commercial fry of the all-male hybrid pelteobagrus fulvidraco. According to the technical scheme, super-female fishes can be efficiently induced in batches, high-density breeding of parent fishes is achieved, and the effect and efficiency of artificial breeding are remarkably improved; the bred hybrid all-realgar catfish is high in individual uniformity, the growth speed is increased by 20%, and the male rate reaches 100%.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The utility model discloses an artificial breeding device for light red ink head fish, which belongs to the technical field of aquatic product hatching and fry breeding and comprises a spawning induction pond, a hatching pond and a fry collecting pond. Cobblestones are laid at the bottom of the spawning induction pond, a spraying mechanism is fixedly connected to the top of the spawning induction pond, an ovulation pipe is communicated with the center of the spawning induction pond, the top of the ovulation pipe is lower than the top face of the spawning induction pond, and the bottom end of the ovulation pipe penetrates through the spawning induction pond to be communicated with the hatching pond; a filtering mechanism is detachably connected to the middle of the hatching pond, an overflow pipe is arranged in the filtering mechanism, and the bottom of the overflow pipe penetrates through the hatching pond to communicate with the outside; the side wall of the bottom of the hatching pond is communicated with a seedling outlet pipe, and the seedling outlet pipe is fixedly connected with a control valve and communicated with the seedling collecting pond. The device can effectively simulate the natural breeding environment of the light red ink head fish, accurately control various key parameters in the breeding process, and improve the reproductive performance of parent fish, the hatching rate of fish eggs and the survival rate of fish fries.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention provides an artificial propagation method of Sinilabeo wuyi, and belongs to the technical field of aquatic animal breeding. The artificial propagation method of the Sinilabeo wuyi comprises the steps of parent fish classification, parent fish rearing, artificial spawning induction, natural insemination, artificial insemination, fertilized egg incubation, fry rearing, juvenile fish rearing and the like. The oxytocic is reasonable in compatibility, the injection time of the oxytocic is accurate, the oxytocic rate, the fertilization rate and the hatching rate are increased, and damage to parent fishes is small. According to the method, feeding methods in different stages of fry breeding are also defined, the survival rate of the sinilabeo wuyi fry breeding stage is increased, and a technical support is laid for artificial breeding of the endangered species sinilabeo wuyi.

The invention discloses a method for efficient maturation of ovaries of industrially cultured plectropomus leopardus. The method comprises the following steps: screening healthy plectropomus leopardus individuals from a plectropomus leopardus population as parent fishes; putting the fish in an indoor culture pond for water adaptation to obtain high-quality parent fish; performing induced culture on the high-quality parent fish, wherein the induced culture comprises nutrient enrichment and temperature induction; the temperature induction time is less than or equal to 35 days to promote the ovary maturation of the parent fish. Through an indoor induction mode combining low-temperature energy storage, gradient rewarming and nutrient enrichment feed, the maturation rate of the female plectropomus leopardus ovary can be remarkably increased to 85% or above, and the maturation period is shortened to be within 40 days. The method is suitable for a standardized breeding process of an indoor parent fish breeding workshop, effectively realizes synchronous maturation and quality improvement of the parent fish group ovary, provides a stable parent guarantee for indoor artificial breeding of plectropomus leopardus, and has a good popularization and application prospect.

The invention provides an artificial breeding method of spinibarbus capito, which is suitable for Dongting Lake in Hunan Province and four water basins of Hunan, Zi, active yuan and halite, and comprises the steps of parent fish rearing, artificial spawning induction and artificial incubation, and spawning can be carried out for 1-2 times in one year. In the spinibarbus capito parent fish rearing process, puffed floating fish feed is used as a main material and properly matched with green feed, animal bait is added for feeding 2 months before winter, accumulation of fish body fat can be guaranteed so that the fish can smoothly pass the winter, vitamin E, vitamin C and flowing water stimulation are supplemented in the feed before antenatal, and ovary development and ovum maturation can be effectively promoted; the artificial spawning induction adopts a two-needle injection method, the spawning induction efficiency is high, and the fertilization rate is high; through comparison of different hatching devices, the hatching effect of the hatching frame is best, the hatching rate reaches 80% or above, the hatching barrel is inferior, and the small net cage is worst. By means of the artificial propagation method, large-scale production of spinibarbus capito can be achieved, and the method is of great significance in improving the economic benefits of farmers and popularizing the spinibarbus capito artificial propagation technology.

The invention provides a polypeptide with an ovary maturation promoting effect and application of the polypeptide in parturition induction of pomfret. The amino acid sequence of the polypeptide is SEQ ID NO: 1. The polypeptide provided by the invention is derived from the GnRH2 gene of the pomfret, and in-vivo experiments prove that the polypeptide can efficiently stimulate the pituitary of the pomfret to secrete gonadotropin, so that the spawning induction time is remarkably shortened, and the spawning rate and the relative spawning amount of parent fish are increased. The polypeptide is strong in species specificity and good in physiological compatibility, does not generate an antibody, avoids side effects of traditional heterologous hormones, is safe and effective, and has a huge potential of being developed into a special spawning induction agent for the pomfret.

The invention discloses a breeding and maturing method of Sichuan parabramis pekinensis parent fish, which comprises the following steps: putting male and female parabramis pekinensis into a breeding pond with the putting density of 150 parabramis pekinensis / m < 2 >; the tubificidae and the sturgeon special feed are uniformly mixed for domestication, the weight ratio of the tubificidae to the sturgeon special feed is 8: 2, and an artificial nutrition feed additive is supplemented; 7-10 days before spawning induction, feeding is stopped; feeding management: the dissolved oxygen in the culture water is greater than 6mg / L, the average water temperature is 23 DEG C, and the pH value is 6.5-7.5; disinfecting the parent fish culture pond every other January; water flow stimulation: adjusting the water flow speed to 0.04 m / s; in the close breeding period, a rainfall temperature increasing and water flow stimulation combined mode is adopted, and water surface and underwater combined mode is adopted for water flow stimulation. According to the method, through artificial regulation and control, the female and male Sichuan parabramis pekinensis parent fishes are mature, the brood amount is large, the reproductive capacity is high, the sperm and egg quality is high, and synchronous maturation of the female and male parent fishes is achieved.

The invention provides an artificial breeding method for echinacea chlamydoides. The artificial breeding method comprises the following steps: S1, selecting parent fish; s2, performing nutrient enrichment culture on the parent fish; s3, artificial spawning and fertilization; s4, artificial incubation; s5, seedling cultivation. The feed is reasonably matched to promote parent fish development and improve egg laying and sperm production quality, spawning hormone is manually injected, injection dosage is strictly controlled, eggs and sperm which are well developed are obtained, hatching conditions of the fertilized eggs are controlled, the hatching rate of the fertilized eggs reaches 90% or above, and the hatching rate of the fertilized eggs reaches 90% or above. According to the method, oyster fertilized eggs, rotifers, artemia or cladocerans / copepods nauplii, cladocerans / copepods and floating type larva fry breeding seawater fish compound feed are sequentially fed in the fry breeding period, palatable bait series of different fry breeding stages are established, and a mature fry breeding technical route is formed.

The invention discloses a bionic spawning site suitable for producing super-male opsariichthys bidens fry by pseudo-female opsariichthys bidens, and particularly relates to the field of artificial breeding of all-male opsariichthys bidens. The egg laying and collecting device comprises a simulated ecological running water sand bottom egg laying site and an opening and closing mechanism for controlling quartz sand to fall after egg laying; the opening and closing mechanism comprises a sliding rail rod and a sealing sliding plate, the sliding rail rod is fixedly arranged at the lower end of the spawning pond, and the sealing sliding plate is connected to the sliding rail rod in a sliding mode. The bionic spawning site can simulate the requirements of spawning behaviors of opsariichthys bidens on sand bottoms and flowing water under natural conditions, avoids damage to parent fishes of pseudo female opsariichthys bidens caused by manual spawning, and improves the survival rate of the postpartum pseudo female parent fishes.

The invention provides a domestication method of wild sal-family large yellow croaker fries, and belongs to the technical field of aquaculture. The method comprises the following steps: capturing wild fry of the halitium larimichthys crocea, transporting the wild fry to a culture pond for temporary culture, and then sequentially carrying out live bait domestication, chilled bait domestication, soft pellet feed domestication and artificial matched pellet feed domestication culture. According to the method, live baits loved by the wild sal-family pseudosciaena crocea are fed and are gradually transited to special artificial compound feed, so that the feeding habit and digestive system of the wild sal-family pseudosciaena crocea are gradually adapted, the wild sal-family pseudosciaena crocea can be successfully converted to eat the artificial compound feed after being domesticated for 20 days, the survival rate reaches 63% or above, and the phenomenon of batch death does not occur during domestication. According to the method, the survival rate of the fries domesticated from the wild sal-family large yellow croaker fries into the fries under the artificial breeding condition is effectively increased, and a guarantee is provided for building an artificial breeding basic parent fish group.

The artificial breeding method comprises the following steps: parent fish culture: selecting wild cymbidium for cage culture, and feeding culture bait; after the weight of the parent fishes reaches the standard that the weight of the female fishes is larger than 200 g / fish and the weight of the male fishes is larger than 150 g / fish, round fishes which are robust in physique, lively in action, high in stress resistance and free of body surface trauma are selected as the parent fishes, the parent fishes are transferred into the parent fish pond from the net cage, and the proportion of the female parent fishes to the male parent fishes is (2-2.5): 1. According to the method disclosed by the invention, the fortified bait prepared from the iced trash fish, the eel meal, the probiotic, the multivitamins, the fish oil, the vitality-promoting agent and the yeast is used for fortifying the parent fish of the Cymbidium, and a certain dosage of HCG is used for carrying out ripening and spawning induction treatment on the female parent and the male parent, so that the Cymbidium fry can be obtained on a large scale; therefore, the artificial breeding of the cynanchum otophyllum fries is realized, the blank of the artificial breeding technology of the cynanchum otophyllum is filled, a foundation is provided for the large-scale production of the cynanchum otophyllum fries, and the popularization of

The invention discloses a method for regulating out-of-season breeding of large yellow croakers bred in autumn. The method is characterized in that spawning in spring and summer is achieved through a three-stage technical system of biological safety prevention and control, precise environment regulation and control and reproductive physiological intervention. The method specifically comprises the following steps: 1) detecting trypanosomes; 2) parent fish screening; (3) parent fish rearing; 4) artificial propagation; 5) artificial incubation; and 6) seedling cultivation. According to the method, the out-of-season spring and summer breeding technology breakthrough of breeding the large yellow croaker in autumn is successfully achieved through nutrient enrichment, accurate temperature parameter regulation and control, gradual illumination regulation and reasonable hormone treatment. According to the technology, an adjustable reproduction synchronization scheme is provided for cross breeding between a spring reproduction group and an autumn reproduction group, and the technology has important application value for germplasm innovation of the large yellow croaker.

The invention provides a method for on-site rapid artificial insemination of wild sal-family large yellow croakers, and belongs to the technical field of aquatic animal breeding. According to the method, wild parent fishes are captured in the breeding season of the sal-family pseudosciaena crocea; selecting sexually mature male parent fishes on a capturing site, and collecting seminal fluid for later use; after manual semen collection is finished, female parent fishes with sexual maturity are selected, and fish eggs are collected; performing artificial insemination to obtain high-quality fertilized eggs; the artificial insemination comprises the following steps: adding seminal fluid into the roes, then adding natural seawater, stirring, and finally washing with the natural seawater. According to the method, key links of the whole artificial insemination process are comprehensively regulated and controlled, so that the purposes of obtaining high-quality fertilized eggs and carrying out artificial fry breeding by utilizing the parent fish of the wild sal ammoniac large yellow croaker are achieved.

The invention provides a breeding method of fast-growing high-quality Dongzhihuo. Comprising the following steps: identifying the genotype of an SNP (Single Nucleotide Polymorphism) locus which is located at the 79th site of a gene sequence as shown in SEQ ID NO.1, and if the genotype of the locus is TT type, determining that the locus is a candidate parent; selecting candidate parents with uniform and robust body types as parent fishes; carrying out spawning induction on the male fish and the female fish of the Dongstellate star; collecting ova and sperms for artificial insemination; uniformly putting the fertilized eggs into an indoor nursery pond for hatching and breeding. The euryale ferox bred through the method is high in growth and development speed, high in muscle fat content, high in crude protein content, high in flavor amino acid content, delicious in taste, more popular with consumers and good in market prospect.