21 results about "Rainbow trout" patented technology

The utility model discloses a device for fixing fertilized eggs of rainbow trout, which belongs to the technical field of aquaculture and comprises a box body, the top of the box body is fixedly connected with a pair of slide rails, and each slide rail is slidably connected with a support frame. By arranging the sliding rail, the supporting frame, the first bevel gear, the first threaded rod and the transmission assembly, the position of the egg box is adjusted, the supporting frame can slide on the sliding rail and drive the egg box to move horizontally, the transmission assembly drives the first bevel gear to rotate, the first threaded rod can drive the egg box to ascend and descend, it is ensured that the egg box is in a suitable environment, and the hatching rate is increased; through the arrangement of the connecting plate and the bolt assembly, the mode that the egg box is rapidly assembled and disassembled, the sliding blocks are matched with the sliding grooves and the bolt assembly is fixed is achieved, the stability of the egg box after installation is guaranteed, the risk that fertilized eggs are damaged due to shaking is reduced, the egg box can be rapidly disassembled as needed, and the egg box can be conveniently cleaned, overhauled or replaced.

The present application relates to rainbow trout nucleic acid vaccine adjuvant, the adjuvant includes polydisulfide compound and cyclic di-GMP delivered by polydisulfide compound, the present application also relates to the application of the rainbow trout nucleic acid vaccine adjuvant in the preparation of rainbow trout IHNV virus nucleic acid vaccine.A kind of rainbow trout nucleic acid vaccine adjuvant, i.e.c-di-GMP+CPD complex, is developed based on STING agonist-c-di-GMP and polydisulfide compound (CPD) delivery carrier, so as to realize the cost reduction and efficiency increase of rainbow trout nucleic acid vaccine, polydisulfide compound (CPD) can efficiently deliver cyclic di-GMP, and have good stability, c-di-GMP+CPD complex has the potential as rainbow trout immune adjuvant, can significantly enhance the immune protection effect of rainbow trout nucleic acid vaccine, reduces vaccine dosage.

The invention provides a pharmaceutical composition for improving bone mineral density and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises lactarius vomica extract peptide, winged yam rhizome peel extract, rainbow trout extract, calcium citrate-glycine chelate, vitamin K2 and vitamin D3. The lactarius volemus extract peptide is obtained by sequentially carrying out enzymolysis on lactarius volemus by bromelain and subtilisin and purifying and extracting enzymatic hydrolysate through an LH-60 sephadex column. The winged yam rhizome peel extract is obtained by extracting the peels of winged yam rhizome tubers with an ethanol water solution and purifying and extracting the extracting solution with an AB-8 macroporous adsorption resin column. The rainbow trout extract is a product below 5kDa obtained by sequentially performing enzymolysis on fish heads, fish skins and fish scales of rainbow trout through trypsin and lumbrukinase. All the components are compounded according to a specific proportion, and the core effect of improving the bone mineral density is achieved through two-way regulation and control of bone metabolism, optimization of calcium utilization and improvement of safety.

The invention discloses a primer combination for identifying 11 animal-derived components and application thereof, the primer combination comprises 16 specific primers, the nucleotide sequences of the primers are shown as SEQ ID NO.1-16, and the primer combination can be used for identifying 11 animal-derived components of salmon, cod, rainbow trout, oil fish, chicken, duck, goose, cattle, horse, sheep and pig. The invention also provides a corresponding detection method and a detection kit or a detection reagent. The technology realizes high-throughput, high-specificity and high-sensitivity detection of the 11 animal-derived components, not only simplifies the operation process and improves the detection efficiency, but also has wide applicability.

This invention relates to a method for trimming the anal fin of rainbow trout fillets, comprising the following steps: S1: The conveyor belt of the trimming device transports the fillets at a predetermined speed. When the fillet reaches image acquisition point A, point cloud data of the fillet is acquired and converted to the coordinate system of the trimming device; S2: The converted point cloud data is preprocessed to obtain the fillet point cloud, and a fillet coordinate system following the fillet's posture is established based on the fillet point cloud; S3: The anal fin region point set is identified based on the geometric features of the point cloud, and the anal fin contour is extracted; S4: The extracted anal fin contour is curve-fitted, and a predetermined distance is offset along the normal vector direction of the fitted curve to generate a cutting path, and the mapping relationship between the cutting path parameters and the cutting time is determined; S5: The cutting path is converted to the coordinate system of the trimming device in real time, and the cutting device is controlled to cut the moving rainbow trout fillet at trimming station B. This method improves the consistency of trimming and the meat yield, and also significantly reduces labor costs and labor intensity.

The invention discloses a triploid rainbow trout juvenile fish feed and application thereof. The formula of the feed comprises 300.0 g / kg of fish meal, 120.0 g / kg of krill meal, 100.0 g / kg of soybean meal, 80.0 g / kg of soybean protein concentrate, 80.0 g / kg of corn protein, 20.0 g / kg of wheat bran powder, 165.4 g / kg of wheat flour and a mixture of different proportions of fish oil and dephenolized cottonseed oil, the proportion of the dephenolized cottonseed oil replacing the fish oil in the mixture is 25%-100%, and 10.0 g / kg of premix, 0.2 g / kg of astaxanthin, 3.0 g / kg of choline chloride, 1.0 g / kg of EDTA, 0.3 g / kg of betaine and 0.1 g / kg of calcium propionate are added. The feed disclosed by the invention is applied to culture of juvenile triploid rainbow trout, and can be used for remarkably regulating fatty acid composition of a fish body, improving muscle texture and enhancing oxidation resistance and heat stress resistance of the liver on the premise of not influencing growth performance, so that dependence on fish oil is reduced, and green, efficient and sustainable development of aquaculture is promoted.

The invention provides a rainbow trout recirculating aquaculture, spawning and hatching integrated device, and belongs to the technical field of aquaculture, the rainbow trout recirculating aquaculture, spawning and hatching integrated device comprises an aquaculture device body, a vibration turbulent flow assembly and a circulating air supply assembly; through cooperation of the generation chamber, the incubation chamber and the outlet cavity and a hydraulic transfer system, connection from spawning, fertilization to incubation of rainbow trout is achieved, ovum transfer damage is reduced, and the initial incubation rate is increased; a shading structure is designed to shield external strong light and protect embryonic development; the filter screen is matched with the directional nozzle, and the vibration turbulent flow assembly is combined, so that self-cleaning of the filter screen is achieved, pollution sources are directionally discharged, and hatching water is kept clean; according to the circulating air supply assembly, air is continuously supplied to the upper portion of water in the incubation chamber through a rotating wheel, bubbles are pressed downwards through water flow sprayed obliquely downwards, mixed dive flow is formed, oxygenation can be achieved without extra electric power, the water is stirred through the generated bubbles, the temperature and dissolved oxygen in the incubation chamber are distributed more evenly, and the incubation environment is further optimized.

The present application relates to a new application of radix cirsii japonici extract in resisting largemouth bass iridovirus. The present application finds that the radix cirsii japonici extract has a significant effect in resisting largemouth bass iridovirus, can significantly reduce the copy number of largemouth bass iridovirus, inhibit the expression of largemouth bass iridovirus MCP protein, improve the phagocytic ability of largemouth bass kidney leukocytes, and effectively improve the survival rate of fish infected with largemouth bass iridovirus, and has good biological safety. Therefore, the radix cirsii japonici extract can be used for preparing products such as drugs, fish feed or fish feed additives resisting largemouth bass iridovirus, and provides an effective prevention and control means for largemouth bass and other fish breeding.

The present application relates to the field of aquatic organism breeding technology, and particularly relates to a method for preparing rainbow trout tetraploid fry. The method provided by the present application comprises the following steps: obtaining fertilized eggs by dry fertilization of naturally matured rainbow trout parents, inducing the rainbow trout fertilized eggs to obtain tetraploid rainbow trout after 6.2-6.4 hours, and the duration of the induction is 10-18 minutes; after the tetraploid rainbow trout fry is obtained by induction, the chromosome ploidy of the fish is determined by using techniques such as chromosome karyotype analysis and flow cytometry DNA content determination, and the tetraploid rainbow trout fry is identified and screened for cultivation. The present application adopts a drug induction method, and controls the starting time and duration of the induction to inhibit the first mitosis time of the fertilized eggs, thereby improving the induction rate and survival rate of the tetraploid rainbow trout, and the induction rate of the tetraploid rainbow trout can reach 25-30%, and the survival rate of the fry can reach more than 90%.

The invention provides a triploid rainbow trout refrigeration and fresh-keeping method by combining plant extracts with a low-temperature plasma technology, and belongs to the technical field of rainbow trout refrigeration and fresh-keeping. Hawthorn, pericarpium citri reticulatae and fried semen raphani are mixed according to the mass ratio of 2: 1: 2 to prepare a composite plant fresh-keeping agent; according to a sequence of soaking for 20 minutes with plasma activated water, soaking for 10 minutes with a composite plant preservative, and then carrying out dielectric barrier discharge treatment for 2 minutes, a flavonoid compound and organic acid are used for removing strong oxidizing substances generated by plasma treatment, and an anti-oxidation protection layer is formed; the soaking time of the composite plant preservative is dynamically adjusted according to the thiobarbituric acid value, and the technical problem that the preservation effect is reduced due to the fact that low-temperature plasma treatment accelerates lipid oxidation of the triploid rainbow trout is solved.

The present application relates to the technical field of aquaculture feed, and provides a hermetia illucens protein peptide and a preparation method thereof.The hermetia illucens protein peptide prepared by using the technical solution of the present application can be used as a feed protein source, replace most of the fish meal in the feed, reduce the use of fish meal in the aquaculture feed, and alleviate the problem of fish meal shortage and insufficient supply.The present application also provides application of the hermetia illucens protein peptide in preparation of functional feed for improving the growth performance of fish and improving the muscle quality of fish.The functional feed for fish prepared by using the hermetia illucens protein peptide of the present application can not only significantly improve the growth of rainbow trout, but also has no negative effect on the antioxidant capacity of the liver and back muscle of animals, and can also improve the muscle quality of rainbow trout.

This invention relates to a method and trimming device for recognizing the anal fin contour of rainbow trout fillets. The method includes: S1: acquiring point cloud data of the fish fillet and transforming the point cloud data from the camera coordinate system to the trimming device coordinate system; S2: preprocessing the transformed point cloud data to obtain the fish fillet point cloud, calculating the principal axis direction of the fish fillet point cloud using principal component analysis, and establishing a fish fillet coordinate system that follows the fish fillet posture; S3: extracting the anal fin contour in the fish fillet coordinate system, which includes: calculating the normal vector and curvature of each point in the fish fillet point cloud; selecting a set of point clouds belonging to the anal fin region from the fish fillet point cloud according to preset curvature thresholds, height thresholds, and width thresholds; selecting seed points in the point cloud set, performing region growing based on the spatial distance and normal vector consistency of the point cloud, obtaining the anal fin region point set, and extracting the anal fin contour from it. This method can effectively overcome the influence of water stain reflection, blood stain interference, and uneven lighting on the fish fillet surface on the recognition results.

The application discloses application of Prunella vulgaris extract in prevention and treatment of fish parasitic disease infection. Inclined tube worm in-vitro soaking experiments show that the insecticidal effect of Prunella vulgaris extract and zinc sulfate is equivalent after 48 hours, and the mortality rate is above 95.44%; based on the growth condition of rainbow trout feeding on Prunella vulgaris extract, the specific growth rate of fish is significantly improved, the growth performance is improved, and the growth of fish is promoted; the plasma biochemical indexes after parasitic infection show that the addition of 0.25% to 1% Prunella vulgaris extract in feed can significantly reduce stress caused by pathogenic stress and protect the health of fish liver.

The invention discloses a special constant-temperature hatching device for rainbow trout fry rearing, which relates to the technical field of aquaculture equipment and comprises an external heat insulation box body, a plurality of layers of independent hatching units, a water circulation control system, a main water inlet pipe and a water return pipeline. The hatching unit is of a drawer type structure, the bottom of the hatching unit is provided with a flow equalizing plate and a conical flow guide hole, a bottom-up upwelling type suspension flow field is formed, and roes are evenly suspended and rolled. The water circulation system integrates a circulating pump, a refrigerating and heating module, an ultraviolet sterilizer and a dissolved oxygen oxygenation device, and accurate control over water temperature, control over the content of dissolved oxygen and continuous sterilization are achieved. The upper part of each unit is provided with an overflow port, and water flow is filtered by a water return pipeline and recycled to form a closed-loop system. The device has the advantages of constant temperature stability, uniform dissolved oxygen, convenience in operation, high space utilization rate and the like, can remarkably improve the hatching rate of rainbow trout eggs and fry quality, and is suitable for large-scale breeding production.

This invention discloses a method for deodorizing rainbow trout skin using a blanching-tea polyphenol coupling process. The method includes the following steps: S1. The fish skin is frozen and thawed at 4°C before use. After thawing, the muscle tissue attached to the surface of the fish skin is mechanically scraped off to remove residual meat scraps and impurities, and then the fish skin is washed. S2. The fish skin treated in step S1 is cut into fish skin samples of the same shape according to the same specifications. S3. The fish skin cut in step S2 is blanched in pure water at a medium temperature for a short time. S4. The fish skin blanched in step S3 is soaked in a tea polyphenol solution of a certain concentration at room temperature for 60 minutes and then drained. This invention is green, natural, and deeply deodorizes the fish. The blanching-tea polyphenol coupling process effectively improves the defects of heavy residual fishy smell and high risk of undesirable residues, and has broad application prospects in the field of high-value utilization of fish by-products.

The invention relates to the technical field of aquaculture, in particular to an efficient and low-consumption cold water shrimp fry and fingerling breeding method. The method comprises the following steps: laying microcapsules for packaging paraffin phase-change materials at the bottom of a breeding pool, injecting underground water in a pulse manner through an annular water distribution pipeline, monitoring the water temperature in real time by using a wireless temperature sensor, and regulating and controlling the water temperature in combination with intelligent temperature control logic. According to the invention, temperature fluctuation is buffered bidirectionally through heat absorption and heat release of the phase-change material, and an underground water pulse injection technology is combined, so that the problems of high embryo death rate and over-high energy consumption caused by temperature fluctuation and embryo displacement damage caused by water flow disturbance in the prior art are solved, and the embryo death rate and energy consumption are remarkably reduced; the device is suitable for hatching cold water fries such as rainbow trout and cold water shrimps, and has the advantages of high stability, good economical efficiency and strong ecological property.

The invention discloses a cryobacterium SD-C-1-7 and application thereof in rainbow trout culture, and belongs to the technical field of aquaculture water quality purification. The strain is classified and named as Pseudomonas sp., when the strain is in a low-temperature environment of 8-18 DEG C, the removal rate of ammonia nitrogen in a rainbow trout culture water body is larger than or equal to 87%, the removal rate of nitrite nitrogen is larger than or equal to 91%, the removal rate of total phosphorus is larger than or equal to 52%, and the preservation number is CCTCC NO: M20251642. The method has the core advantages that the low-temperature adaptability is high, and the problem of low low-temperature denitrification efficiency of traditional nitrifying bacteria is solved; the purification function is comprehensive, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen can be synchronously removed, and total phosphorus can be synergistically reduced; the purification efficiency is stable, in a 60-day rainbow trout breeding test, ammonia nitrogen is stabilized to be 0.4 mg / L or below, nitrite nitrogen is lower than 0.2 mg / L, and the average removal rate of total nitrogen and the average removal rate of total phosphorus reach 29.86% and 39.45% respectively. Meanwhile, the invention provides a water quality purification fungicide containing the strain and a rainbow trout circulating water culture water quality purification method, the fungicide preparation process is mature, application is convenient, rainbow trout culture benefits are remarkably improved, and the requirements of green sustainable development are met.

The invention relates to the technical field of breeding ponds, and discloses a movable rainbow trout fry breeding pond and a breeding method thereof.The movable rainbow trout fry breeding pond comprises a breeding pond body, an upper mounting frame is arranged above the breeding pond body, two side plates are arranged above the upper mounting frame, two sliding rails are further arranged on the breeding pond body, and electric sliding blocks are slidably arranged on the two sliding rails; connecting blocks are arranged above the two electric sliding blocks, and a mounting plate is arranged above the two connecting blocks. A mounting pipeline is arranged in the middle of the mounting plate in a penetrating mode, a feeding port is formed in the upper portion of the mounting pipeline, and a containing shell is arranged below the mounting pipeline. And two upper sealing plates and two lower sealing plates are arranged at the bottom of the placement shell. According to the invention, feed can be temporarily stored through the feed inlet of the mounting pipeline, the placement shell is matched with the upper sealing plate, the lower sealing plate and the sliding sealing plate, and the side plate of the upper mounting frame can control the opening and closing size of the sliding sealing plate, so that sufficient food at the edge of the breeding pond is ensured, and the breeding pond is adaptive to the fry gathering habit.

The invention provides an intelligent AI plateau rainbow trout modernized breeding method, which comprises the following steps: collecting water environment and fish body parameters in real time through a multi-source sensor, generating a multi-modal feature vector through plateau specificity data fusion, inputting a plateau adaptability AI decision model for risk assessment and growth stage recognition, and generating a precise control instruction. Equipment for oxygenation, feeding, water flow and the like is cooperatively regulated and controlled, and model parameters are dynamically optimized based on a stress feedback mechanism, so that high-efficiency and low-stress intelligent culture of the plateau rainbow trout is realized. The plateau rainbow trout breeding system can improve the breeding efficiency, reduce the stress risk, optimize the energy consumption, and improve the intelligent level and economic benefits of plateau rainbow trout breeding.

The invention relates to application of astragalus polysaccharide in preparation of a medicine for preventing and treating infectious hematopoietic necrosis, and belongs to the technical field of disease prevention and treatment in aquaculture. The astragalus polysaccharide is mainly from leguminous plants and is light yellow powder. Tests prove that the astragalus polysaccharide has a remarkable inhibiting effect on IHNV, can prevent rainbow trout from being infected with IHNV, and remarkably improves the survival rate of diseased fish. The astragalus polysaccharide disclosed by the invention is wide in source, low in price and small in toxic and side effects, is used for preventing and controlling IHNV infection in production practice, and has a wide market prospect.

The invention discloses a triple RT-qPCR method and kit for simultaneously detecting rainbow trout IHNV, VHSV and vibrio anguillarum, and belongs to the technical field of pathogen detection. According to the invention, qPCR primers and TaqMan probes for detecting IHNV, VHSV and vibrio anguillarum are respectively designed, and the nucleotide sequences of the qPCR primers and TaqMan probes are shown as SEQ ID NO: 1-9. The primer and the probe can be used for simultaneously qualitatively and quantitatively detecting three pathogens, namely IHNV, VHSV and vibrio anguillarum, by virtue of a triple qPCR (Quantitative Polymerase Chain Reaction) method. The triple RT-qPCR method for simultaneously detecting the IHNV, the VHSV and the vibrio anguillarum is established for the first time, common detection of RNA viruses and bacteria is realized, and the method has the characteristics of high efficiency and convenience; meanwhile, the molecular crowding agent is added as a component for improving the amplification efficiency, so that the detection of pathogens in a medium-low concentration range is ensured, and the reliability of the detection limit is improved. The method provided by the invention has no amplification curve for non-target pathogens, and has good specificity.