50 results about "Vitamin K2" patented technology

The present disclosure relates to the synthesis of Vitamin K2 bearing varying prenyl side chains. The synthesis comprises the reaction of phenyl sulfone of tert-butyl dimethyl silyl (TBDMS) protected mono prenyl menadiol with prenyl halides bearing varying prenyl units in the presence of the phase transfer catalyst followed by reductive desulphonation to yield TBDMS ether of Vitamin K2. The TBDMS ether is then oxidized in the presence of chromium trioxide and periodic acid to yield the corresponding Vitamin K2.

The invention relates to the field of detection, in particular to a method for simultaneously detecting multiple fat-soluble vitamins, which comprises the following steps: (S.1) carrying out protein precipitation and liquid-liquid extraction on a sample to be detected to obtain an extract containing fat-soluble vitamins; (S.2) carrying out derivatization reaction on the extract by using a PTAD derivatization reagent; and (S.3) analyzing the derivatized product by adopting a liquid chromatography-tandem mass spectrometry method, wherein the detected target fat-soluble vitamins comprise vitamin A, vitamin E, 25-hydroxy vitamin D2, 25-hydroxy vitamin D3, vitamin K1, vitamin K2 and vitamin K2. According to the application, PTAD is creatively adopted as a unified derivatization reagent, simultaneous and efficient derivatization of seven fat-soluble vitamins is successfully realized, the detection sensitivity of low-content targets, especially vitamin D, K2 and the like, is greatly improved, and the core problems of weak signal response and difficulty in accurate quantification are solved.

This invention belongs to the field of biotechnology and discloses a recombinant strain of Saccharomyces cerevisiae that enhances the efficient synthesis of vitamin K2 and its application. By heterologously integrating the hepS / T and naphthoquinone cyclotransferase genes (menA) of Bacillus subtilis, four genetically engineered strains were constructed based on the designed MK-7 biosynthetic pathway: YK-1, YK-2, YK-3, and YK-4. YK-1 is an expression strain containing the target genes MenF, MenD, and MenH; YK-2 is an expression strain integrating the target genes MenC, MenE, and MenB on the basis of YK-1; YK-3 is an expression strain integrating the target genes hepS and hepT on the basis of YK-2; and YK-4 is an expression strain integrating the target genes yuxO, MenA, and MenG on the basis of YK-3. YK-4 is the final engineered strain that produces high levels of vitamin K2. The optimal fermentation time was determined by measuring OD600 and yield curves. YK-4 reached its maximum cell concentration in 18 hours, which can effectively shorten the fermentation cycle.

The invention provides a pharmaceutical composition for improving bone mineral density and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises lactarius vomica extract peptide, winged yam rhizome peel extract, rainbow trout extract, calcium citrate-glycine chelate, vitamin K2 and vitamin D3. The lactarius volemus extract peptide is obtained by sequentially carrying out enzymolysis on lactarius volemus by bromelain and subtilisin and purifying and extracting enzymatic hydrolysate through an LH-60 sephadex column. The winged yam rhizome peel extract is obtained by extracting the peels of winged yam rhizome tubers with an ethanol water solution and purifying and extracting the extracting solution with an AB-8 macroporous adsorption resin column. The rainbow trout extract is a product below 5kDa obtained by sequentially performing enzymolysis on fish heads, fish skins and fish scales of rainbow trout through trypsin and lumbrukinase. All the components are compounded according to a specific proportion, and the core effect of improving the bone mineral density is achieved through two-way regulation and control of bone metabolism, optimization of calcium utilization and improvement of safety.

The invention provides application of a composition containing butyric acid producing bacteria and vitamin K2 in preparation of a medicine for treating osteoporosis, and relates to the technical field of biological medicines, the composition comprises butyric acid producing bacteria and vitamin K2; wherein the butyric acid producing bacteria are selected from any one or more of clostridium butyricum, rosella and clostridium praeparatum; and the vitamin K2 is selected from any one or more of menadione-4 or menadione-7. The composition can play a synergistic effect through an intestine-bone axis, and compared with a single component, the composition can more remarkably improve the bone mineral density. The composition has comprehensive effects and high safety, provides a brand-new nutrition intervention strategy with a synergistic effect for prevention and treatment of osteoporosis, and provides a brand-new possibility for preparation of medicines related to osteoporosis.

The invention relates to the technical field of in-vitro detection, in particular to a sample pretreatment method and a method for detecting VK2 and VD in serum. According to the specific technical scheme, the method comprises the following steps: carrying out protein precipitation after carrying out enzymolysis on serum, taking supernate, mixing the supernate with activated magnetic beads, enabling the magnetic beads to adsorb a target object, finally eluting the magnetic beads adsorbed with the target object by using eluent, collecting the eluent, and detecting the eluent to obtain vitamin K2 (VK2) and 25-hydroxy vitamin D (VD) at the same time. The problems that pretreatment operation is tedious, health is harmed and flux is low are solved by adopting an automatic magnetic bead method; the problems that detection equipment is expensive and difficult to popularize are solved by adapting to a conventional mass spectrum platform; the technical path is not only suitable for detection of vitamin K2 and 25-hydroxyvitamin D, but also provides a solution normal form with popularization value for detection of trace micromolecular markers which are difficult to popularize due to complex pretreatment or high equipment threshold.

The present application relates to the technical field of nutritional supplement food, more specifically, to a double-protein balanced type of old-age nutritional supplement food and a preparation method thereof. The food comprises calcium, magnesium, zinc, iron, vitamin C, vitamin B6, vitamin E, vitamin B2, sodium selenite, nicotinamide, vitamin D3, vitamin A, vitamin B12, folic acid, vitamin B1 and vitamin K2. The present application is targeted at physiological characteristics and metabolic rules of middle-aged and old people, and scientifically proportioned various vitamins, minerals, essential fatty acids and antioxidant substances required by human body, so as to fully meet daily nutritional requirements of the middle-aged and old people and make up for insufficient dietary intake caused by decreased digestive and absorptive functions.

The invention relates to fermented milk containing human milk oligosaccharide 2-FL and having a bone mineral density improving function. The invention provides application of human milk oligosaccharide 2-FL in preparation of a composition for improving bone health, and further provides a composition which contains the human milk oligosaccharide 2-FL and has a function of improving bone health. The composition comprises human milk oligosaccharide 2-FL and a leavening agent, wherein the leavening agent is one or a mixture of more of streptococcus thermophilus, lactobacillus bulgaricus and a vitamin K2-producing leavening agent. Compared with other technical schemes of the same type for enhancing the bone mineral density, the calcium intake of a subject is not additionally increased, the risk that the subject suffers from organ calculi is reduced, the application range is wider, and the preparation method is suitable for being applied to various fermented milk products.

This invention discloses a desmethylmenoprenone methyltransferase mutant, its construction method, and its applications, belonging to the field of biotechnology. Desmethylmenoprenone methyltransferase is a key enzyme in the MK-7 biosynthetic pathway, responsible for catalyzing the methylation reaction of desmethylmenoprenone to generate a series of methylnaphthoquinone (MK) products, ultimately forming MK-7. This invention, through metabolic network regulation and rational design, successfully obtained 19 single-point mutants, among which 5 mutants showed significantly higher catalytic efficiency than the wild-type enzyme. Experimental results show that the recombinant engineered bacteria constructed based on these mutants exhibit significant advantages in the whole-cell catalytic production of vitamin K2 (MK-7), with MK-7 yield increased by more than 20% compared to the wild-type strain. The excellent catalytic performance of the menG mutant provided by this invention provides strong technical support for the industrial production of vitamin K2, and has broad application prospects and market value.

Composition comprising a mineral combination: calcium, magnesium, zinc; an amino acid combination: L-lysine, L-tryptophan or other amino acids; a vitamin combination: vitamin D3, vitamin K2, vitamin A; a bioactive peptide combination: casein phosphopeptide, collagen tripeptides, egg yolk tripeptides / hydrolyzed egg yolk flour.

The invention belongs to the technical field of inhalation preparations and pulmonary drug delivery, and particularly relates to an atomization preparation for vitamin K inhalation drug delivery and preparation and application thereof. According to the preparation, dipalmitoyl phosphatidylcholine, cholesterol and optional anionic phospholipid are adopted to construct a bionic lung surface activity liposome skeleton LA1, an MK-7 organic phase is intercalated into a liposome double-layer hydrophobic area under the condition of controlling temperature and an energy path, and a continuous phase containing sodium chloride, propylene glycol and a pH regulator is matched to form an isotonic near-neutral environment; a neutral oil phase, a fat-soluble antioxidant, an optional metal chelating agent and a viscosity modifier can be introduced to synergistically optimize the atomization performance. The apparent viscosity of the prepared atomization preparation at 25 DEG C is 1.0-2.5 mPa.s, the osmotic pressure is 280-320 mOsm / kg, the average particle size of lipidosome Z is 80-150 nm, the PDI is not larger than 0.25, the mass median diameter of fog drops atomized by a vibration net type atomizer is 2.0-3.0 microns, the fine particle fraction is not smaller than 60%, and the MK-7 content retention rate is not lower than 90% after 6 months of accelerated storage at 25-40 DEG C.

The invention provides a micro-fat calcium supplement carrier, which is prepared from the following raw materials in parts by weight: 30 to 35 parts of standard index calcium, 1 to 5 parts of chondroitin, 1 to 5 parts of glucosamine, 8 to 12 parts of pure camel milk, 0.7 part of vitamin D, 2 to 70.3 parts of vitamin K, 1 to 3 parts of phospholipid complex and 8 to 10 parts of excipient. According to the invention, standard calcium, chondroitin, glucosamine, pure camel milk, vitamin D, vitamin K2-7, a phospholipid complex and an excipient are organically mixed by adopting a liposome carrier technology, so that the digestion and absorption of calcium in a body and the distribution of calcium in a specific region are effectively improved, and further the effects of rapid bone growth, increase of bone length (promotion of height increase) and increase of bone density are achieved; the health-care food has the health-care functions of reducing the fracture probability, preventing tooth decay, reducing the death rate of cardiovascular diseases and the like, so that the calcium absorption rate is remarkably improved and reaches up to 95%.

The present disclosure relates to an improved method for regiospecific and stereospecific synthesis of polyisopentenyl compounds. More particularly, it discloses regiospecific and stereospecific synthesis of (2E, 6E, 10E, 14E, 18E, 22E)-3, 7, 11, 15, 19, 23, 27-heptamethyl octacosa-2, 6, 10, 14, 18, 22, 26 heptaen-1-ol useful for the synthesis of vitamin K2-7.

The application provides a vitamin premix and a preparation process thereof, and belongs to the technical field of food additives, and the preparation process is as follows: adding mixed microparticles of fat-soluble vitamins, mixed water-soluble vitamins and mixed minerals into a mixer and mixing, then adding vitamin C, magnesium taurate, amino acids and tricalcium phosphate and continuing to mix, and sieving to obtain the vitamin premix. The raw materials of the mixed microparticles of fat-soluble vitamins include vitamin A, vitamin E acetate, vitamin D3, vitamin K2, acacia, modified protein and beta-cyclodextrin; the raw materials of the mixed water-soluble vitamins include vitamin B1, vitamin B2, vitamin B5, vitamin B12, folic acid and nicotinamide; and the raw materials of the mixed minerals include ferrous glycinate, calcium citrate, magnesium citrate and zinc glycinate. The vitamin premix prepared by the application can be used in various foods as a nutritional supplement, is convenient to use, and has good stability and mixing uniformity.

The invention provides bacillus subtilis and application thereof in fermentation production of vitamin K2, the bacillus subtilis is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.35348, and the preservation date is July 28, 2025; according to the application of the bacillus subtilis in fermentation production of the vitamin K2, the unit yield of the vitamin K2 can be increased, and particularly, the vitamin K2 is the vitamin K2 (MK-7). When the bacillus subtilis is applied to fermentation production of the vitamin K2, the yield of the vitamin K2 (MK-7) can be increased, the average yield reaches up to 400 mg / L or above, the operation procedure is simple, the method is stable, reproducibility is good, and industrial production can be achieved easily.

Compositions comprising nanoparticles of vitamin K2 and methods of use thereof are disclosed.

The invention belongs to the technical field of livestock and poultry breeding and feed additives, and particularly relates to a nutrition additive for prolonging the shelf life of eggs and application of the nutrition additive. The nutritional additive for prolonging the shelf life of the eggs consists of the following active ingredients in parts by weight: 5-10 parts of compound organic trace elements, 5-30 parts of in-vivo antioxidant compound substances, 10-30 parts of an intestinal health regulator, 0.1-1 part of organic selenium, 1-5 parts of vitamin E, 3-10 parts of vitamin C, 0.1-2 parts of vitamin K2, 30-60 parts of functional fat powder and 5-30 parts of a carrier. According to the additive disclosed by the invention, through scientific compatibility of specific nutrients and in-vivo biotransformation of laying hens, the deterioration resistance of eggs is fundamentally enhanced. After the additive is used, the shelf life of the eggs stored at normal temperature can be prolonged to more than 5 weeks.

The invention relates to the field of functional food preparation by a fermentation method, and discloses a preparation method and application of vitamin K2 and tea polyphenol reinforced fermented bean flour. The method comprises the following steps: activating bacillus natto serving as a starting strain into primary seeds, culturing the primary seeds in a seed culture medium at 37 DEG C for 12 hours according to an inoculum size of 1% to realize secondary seed culture, then culturing the secondary seeds in a fermentation culture medium containing bean flour and herb tea raw materials according to an inoculum size of 2%, culturing the secondary seeds at 40 DEG C for 6 days to obtain fermentation liquor, and filtering the fermentation liquor to obtain the finished product. And finally, adding a carrier into the fermentation liquid, and performing low-temperature freeze drying or spray drying. Different bean flour and herb tea raw materials are added into a fermentation culture medium, synchronous enrichment of vitamin K2 and tea polyphenol is achieved, and the obtained fermented bean flour is suitable for functional food, brewing drinks, nutrient enrichment ingredients and the like. The technological process is simple, parameters are clear, and amplification and industrial application are facilitated.

The present invention pertains to the use of a live Saccharomyces cerevisiae strain for the treatment and / or prevention of bone-loss disorders. The present invention further pertains to a combination of a live Saccharomyces cerevisiae strain with vitamin K2 for the treatment and / or prevention of bone- loss disorders such as osteoporosis. The present inventors have shown that such a combination allows significantly improving bone health markers while reducing negative markers associated with osteoclastogenic activity.

The invention provides application of vitamin K2 in biphasic regulation of ER-alpha36 expression and hepatoma carcinoma cell growth, and belongs to the technical field of medicines.The vitamin K2 has an estrogen-like effect and can biphasic regulate hepatoma carcinoma cell growth through an ER-alpha36 signal transduction pathway, that is, low-dose vitamin K2 up-regulates ER-alpha36 expression and promotes HepG2 cell growth, so that the hepatoma carcinoma cell growth is promoted. A high dose of vitamin K2 down-regulates the expression of ER-alpha 36 and inhibits the growth of HepG2 cells. The new application of the vitamin K2 is found for the first time, and a new strategy is provided for treatment of hepatocellular carcinoma and drug development.

According to a first aspect of the invention, there is provided health supplement composition comprising: a therapeutically effective amount of at least one active ingredient selected from Calcium Carbonate, Maize Starch, Magnesium Stearate, Microcrystalline Cellulose, Vitamin D3, and Vitamin K2; and at least one pharmaceutically acceptable excipient selected from the group consisting of binders, disintegrants, stabilizers, lubricants, diluents, and preservatives, wherein the composition is formulated for oral administration in a powder, tablet, capsule, or liquid dosage form.In an embodiment, the composition is provided in a powder / granular form, typically packaged in tablets, as well as in a liquid form or as an additive to a liquid such as water, juice, or vegetable glycerine. Other delivery forms for the novel supplement may be utilized to meet particular operating circumstances or environments. Consequently, the invention is not limited to a specific delivery system but covers any and all suitable delivery systems.

The invention provides a high-dissolution-rate and high-absorptivity liquid calcium preparation as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: carrying out superfine grinding on food-grade calcium citrate, carrying out ultrasonic treatment, loading in milk exosome, mixing with liposome loaded with vitamin K2 and vitamin D3, carrying out ultrasonic treatment, incubating to obtain a fusion body, mixing the fusion body with collagen peptide chelated with calcium, carrying out ultrahigh-pressure homogenization treatment, filtering, sterilizing and filling to obtain the collagen peptide containing vitamin K2 and vitamin D3. The liquid calcium preparation with the high dissolution rate and the high absorption rate is obtained. By improving the production process and the preparation, the liquid calcium preparation product which can stably exist for a long time and is high in dissolution rate and absorptivity is prepared, the absorptivity of calcium supplement function nourishment is improved, the calcium supplement effect of the product is further improved, and the preparation has a good application prospect.

The application discloses a vitamin K2 compound microcapsule system with gastric protection-intestinal sustained release function and a preparation method and application thereof, and relates to the technical field of medicine, in particular to a vitamin K2 compound microcapsule system with gastric protection-intestinal sustained release function, which comprises three layers from inside to outside, namely a core layer, an intermediate layer and an outer layer; the core layer is a zein-shellac hydrophobic core for wrapping vitamin K2; the intermediate layer is a pea protein-pectin electrostatic compound; and the outer layer is a sodium alginate and chitosan double-network gel. The microcapsule system can be closely combined in a gastric acid environment after oral administration, reduces the release of active ingredients in the stomach, then is retained at the intestinal mucosa for a long time, slowly releases the contents, slowly presents the function outside, prolongs the half-life, and improves the effect. Vitamin K2 directly or indirectly participates in and regulates various physiological processes related to health, and plays an important role in maintaining bone health, preventing cardiovascular diseases, protecting nerves, improving metabolism and the like.

The invention discloses a vitamin K2 oil agent, which is prepared from the following components in percentage by mass: 1 to 20.0 percent of vitamin K2, 0.1 to 10.0 percent of antioxidant, 0.1 to 10.0 percent of light protective agent and 60.0 to 98.0 percent of grease. The grease is selected from dicapryl carbonate or isononyl isononanoate or a mixture of the dicapryl carbonate or isononyl isononanoate and other grease, and the light protective agent is selected from caryophyllyl malonate diethylhexyl ester, 2-(4-hydroxy-3, 5-dimethoxy styryl) malonate diisooctyl ester or trimethoxy benzal pentanedione or a mixture of the caryophyllyl malonate diethylhexyl ester, the 2-(4-hydroxy-3, 5-dimethoxy styryl) malonate diisooctyl ester and trimethoxy benzal pentanedione and other light protective agents. The antioxidant is selected from vitamin E or a mixture of vitamin E and other antioxidants. The vitamin K2 oil agent has high activity loading capacity and excellent light / oxidation stability, and the application of the vitamin K2 in cosmetics is promoted.

This invention relates to the field of cell culture technology, specifically to a serum-containing osteogenic induction differentiation medium, a culture method, and its application. The differentiation medium consists of a basal medium and supplementary components. The basal medium is MEMα, and the supplementary components consist of component A and component B. Component A includes fetal bovine serum, L-ascorbic acid-2-phosphate magnesium salt, β-glycerophosphate sodium, dexamethasone, resveratrol, estradiol, strontium chloride, vitamin K2, and sodium butyrate; component B is 1α,25-dihydroxyvitamin D3. This invention exhibits high differentiation efficiency. Mesenchymal stem cells induced using the differentiation medium of this invention show obvious calcium nodules within 7 days, and osteoblast differentiation is achieved within 10-12 days.

The invention relates to the technical field of vitamin preparation, and provides a vitamin K2 extraction device and method.The vitamin K2 extraction device comprises a fermentation tank, the fermentation tank is fixedly installed on a fixed rack, a feeding port is formed in the top of the fermentation tank, the output end of the fermentation tank is communicated with a discharging pipe, a control valve is fixedly installed on the discharging pipe, and the control valve is connected with the feeding port. The stirring shaft is rotatably mounted in the fermentation tank, the sampling barrel is fixedly mounted on the side part of the fermentation tank, the sampling assembly is mounted on the sampling barrel, the driving assembly is mounted at the top of the fermentation tank, and the ventilation assembly is mounted at the top of the fermentation tank; the technical problem that in the prior art, when fermentation liquor is sampled, due to the fact that the fermentation liquor retained in a sampling pipe is difficult to reflect the real change condition in a fermentation tank, the authenticity of the taken-out sample is affected, and the judgment on the fermentation condition is affected is solved.

The present invention provides a pharmaceutical composition for improving the quality of life of the elderly based on vitamin K2, the pharmaceutical composition being composed of composition I and composition II, with a mass part ratio of (0.5-7):1. The present invention also provides a method for preparing the pharmaceutical composition and its application. This pharmaceutical combination has the main effects of anti-cancer and anti-Alzheimer's, and at the same time has the effects of bone strength, osteoporosis improvement, anti-aging, antioxidant, memory and cognition improvement, lipid reduction, liver protection, etc., which can effectively improve the quality of life of the elderly and provide healthy support and protection. The pharmaceutical composition of the present invention has stable activity of each component and high bioavailability, which overcomes the technical defects of current products for the elderly, which have a single function, low absorption and utilization effect, strong gastrointestinal irritation, strong side effects, and poor palatability, and does not have any toxic side effects when taken for a long time.

The disclosure is directed to stabilized aqueous compositions containing calcium (in the form of calcium lactate and calcium gluconate), vitamin D3, and vitamin K2. In addition to calcium, vitamin D3, and vitamin K2, the compositions contain an emulsifier (such as e.g., a polysorbate), an antioxidant (such as e.g., ascorbic acid) and one or more of a sweetener (such as e.g., sucralose and sorbitol), a preservative, a flavoring agent, a pH adjuster, or a combination thereof. The disclosure also includes using the compositions to support bone health.

The application belongs to the technical field of organic synthesis, and relates to a synthesis method of vitamin K2 or a derivative thereof. The synthesis method comprises the following steps: obtaining a compound shown in formula I according to a reaction route shown in the following compound 1 and compound 2; wherein the compound 1 and the compound 2 are subjected to enzyme catalysis reaction (enzymatic reaction) to obtain a compound 3; the enzyme used in the enzyme catalysis reaction is one or more of isopentenyl transferase, geranylgeranyl transferase and heptaprenyl pyrophosphate transferase; and n is a natural number from 0 to 7. The synthesis method has the advantages of shortening a synthesis route, simplifying post-treatment operation, less pollution, mild conditions, high reaction efficiency, low production cost, high product purity and the like.