Acne-removing and repairing composition for promoting microcirculation of acne-sensitive skin and application of acne-removing and repairing composition

Abstract

The invention discloses an acne-removing and repairing composition for promoting microcirculation of acne-sensitive skin and application of the acne-removing and repairing composition, and relates to the technical field of cosmetics. The invention provides an acne removing and repairing composition. The acne removing and repairing composition is prepared from the following components in parts by weight: 0.05 to 1 part of vitamin K2, 0.1 to 1.5 parts of swertia indica extract, 0.1 to 2 parts of guaiai extract, 0.005 to 0.1 part of cerium oxide microspheres and 0.5 to 5 parts of mulberry root extract. Wherein the average particle size of the cerium oxide microspheres is 0.2-3 [mu] m, the cerium oxide microspheres contain Ce < 3 + >, and the molar ratio of the Ce < 3 + > in the cerium oxide microspheres is 30-38%. The invention provides an acne-removing and repairing composition, and the inventor finds that when vitamin K2, swertia indica extract, guaiacol extract, cerium oxide microspheres and mulberry root extract exist at the same time in the actual experiment process, the five components can cooperate with one another and act together from multiple aspects, so that the skin roughness is reduced, and couperose skin and acne are improved.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to an acne-repairing composition for promoting microcirculation of acne-sensitive skin and application thereof. Background Art

[0002] Skin microcirculation is a complex system and an important structure of the skin. It is composed of two parallel vascular plexuses, deep and shallow, which store blood, nourish the skin and maintain the stability of skin microcirculation. As an important part of the skin barrier, changes in skin blood flow are affected by physiological, pathological and environmental factors. Different skin conditions and topical medications can cause changes in skin blood flow and change skin microcirculation. Improving skin microcirculation can enhance skin cell activity, provide skin cells with sufficient nutrients and nutrients, remove cell metabolites and various harmful substances, reduce collagen fiber oxidation, and thus delay skin aging. The detection of skin microcirculation can help us understand the state of the skin, which is of great significance for beauty and skin care. Currently, most treatments for acne-prone skin focus on aspects such as the water-oil balance of the skin. During the treatment process, there is little effect on the transportation or regulation of substances, energy, and biological information between cells and tissues, and the improvement of skin microcirculation is ignored. Microcirculation disorders cause continuous and irreversible damage to skin cells. Microcirculation disorders prevent skin cells from obtaining sufficient oxygen and nutrients, slowing down the skin's renewal metabolism, which is not conducive to skin repair and health, causing intermittent acne, easy recurrence, and skin lesions.

[0003] Therefore, an acne-repairing composition that can increase cell vitality, promote blood circulation, enhance facial skin microcirculation, further reduce skin roughness, and improve red blood streaks and acne has broad application prospects. Summary of the invention

[0004] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an acne-repairing composition that can improve cell vitality, promote blood circulation, enhance facial skin microcirculation, further reduce skin roughness, improve red blood streaks and acne, and promote acne-sensitive skin microcirculation, as well as its preparation method and application.

[0005] To achieve the above object, the technical solution adopted by the present invention is: an acne-repairing composition, comprising the following components in parts by weight: 0.05-1 part of vitamin K2, 0.1-1.5 parts of Swertia indica extract, 0.1-2 parts of Guaiac wood extract, 0.005-0.1 parts of cerium oxide microspheres, and 0.5-5 parts of mulberry root extract; wherein the average particle size of the cerium oxide microspheres is 0.2-3 μm, and the cerium oxide microspheres contain Ce 3+ , the Ce 3+The molar proportion in the cerium oxide microspheres is 30-38%.

[0006] Optionally, the test method for the average particle size of the cerium oxide microspheres is as follows: observe the cerium oxide microspheres under an electron scanning electron microscope to measure the average particle size of the cerium oxide microspheres.

[0007] The preparation method of the cerium oxide microspheres of the present invention is prepared with reference to the following patent, application number: 202310697002.X, invention name: cerium oxide microspheres and their preparation method, application and cosmetics. Specifically, a preparation method of cerium oxide microspheres comprises the following steps:

[0008] mixing a cerium-containing precursor with water to prepare a precursor solution;

[0009] Adding a pH adjuster to the precursor solution to prepare a first solution;

[0010] Adding a dispersion medium to the first solution to prepare a second solution; the mass proportion of the cerium-containing precursor in the second solution is 3-7%;

[0011] The second solution is heated for reaction.

[0012] Preferably, the step of preparing the precursor solution includes at least one of the following conditions:

[0013] (1) the cerium-containing precursor comprises one or more of cerium nitrate hexahydrate and cerium nitrate; (2) the mass volume ratio of the cerium-containing precursor to the water is (20-120) g: (10-60) mL;

[0014] Preferably, the step of preparing the first solution comprises at least one of the following conditions:

[0015] (1) the pH adjuster comprises one or more of acetic acid, formic acid and propionic acid; (2) the mass volume ratio of the cerium-containing precursor to the pH adjuster is (20-120) g: (20-120) mL;

[0016] Preferably, the step of preparing the second solution comprises at least one of the following conditions:

[0017] (1) the dispersion medium comprises ethylene glycol; (2) the mass volume ratio of the cerium-containing precursor to the dispersion medium is (20-120) g: (600-700) mL;

[0018] Preferably, the heating reaction includes at least one of the following conditions:

[0019] (1) The pressure of the heating reaction is 0.5Mpa-4Mpa; (2) The temperature of the heating reaction is 160°C-170°C; (3) The time of the heating reaction is 140min-180min; (4) The heating reaction is carried out in a Hastelloy pressure-resistant reactor;

[0020] Optionally, the heating reaction is carried out in a Hastelloy pressure-resistant reactor with a volume of 1 L.

[0021] The vitamin K2 provided by the present invention is a fat-soluble vitamin in the vitamin K family, which is an important nutrient family and has a significant impact on the normal functions of the human body. It can directly or indirectly participate in and regulate many physiological processes related to skin health; Indian Swertia is the dried whole herb of Indian Swertia, a plant of the genus Swertia in the Gentianaceae family, and is one of the main source medicinal materials of the Tibetan medicine Dida. It is an annual or perennial herb and has antioxidant and anti-inflammatory effects; Cerium oxide microspheres are a type of oxide nanomaterial that is currently being studied more. Cerium oxide microspheres have good biocompatibility, and their surface Ce 3+ and Ce 4+ The ions can be rapidly converted into each other, giving it good redox and self-regeneration properties, and excellent antibacterial activity in wound healing; the root bark of mulberry plants mainly contains flavonoids, coumarins, aromatic benzofuran derivatives, polysaccharides and other chemical components, which have the effects of lowering blood pressure, inhibiting bacteria and relieving pain.

[0022] The present invention provides an acne-repairing composition. The inventors found in the actual experimental process that when vitamin K2, Swertia indica extract, Guaiac extract, cerium oxide microspheres, and mulberry root extract are present at the same time, the five components can cooperate with each other and work together from multiple aspects to reduce skin roughness and improve red blood streaks and acne. Swertia indica extract, mulberry root extract, and guaiac extract work together, have anti-inflammatory and antibacterial properties, can inhibit the growth of acne pathogens such as Propionibacterium acnes, and reduce inflammatory reactions. The active ingredients in Swertia indica extract and mulberry root extract, in synergy with guaiac extract, can soothe and calm the skin, reduce vasodilation, promote local blood circulation, and relieve red blood streaks. The present application also adds vitamin K2 and cerium oxide microspheres, which can further protect vascular endothelial cells, enhance the elasticity and toughness of capillary walls, reduce capillary dilation, and enhance the resistance of capillary walls; at the same time, the addition of vitamin K2 and cerium oxide microspheres can further promote blood circulation, promote the regeneration and repair of skin cells, enhance skin barrier function, and improve skin roughness.

[0023] Preferably, the acne-repairing composition comprises the following components in parts by weight: 0.1-0.8 parts of vitamin K2, 0.6-1.2 parts of Swertia indica extract, 0.8-1.5 parts of Guaiac wood extract, 0.01-0.05 parts of cerium oxide microspheres, and 2-4 parts of mulberry root extract.

[0024] Preferably, in the acne-repairing composition, the total mass percentage of vitamin K2 and cerium oxide microspheres is 2-10%; and / or, in the acne-repairing composition, the mass percentage of Swertia indica extract is 10-20%; and / or, in the acne-repairing composition, the mass percentage of Morus alba extract is 40-60%.

[0025] The inventors found during the actual research process that the weight of the components in the acne-removing and repairing composition will affect the performance of the product. When the weight of the components is further selected to be within the above range, the obtained product has better effects of improving cell vitality, promoting blood circulation, enhancing facial skin microcirculation, further reducing skin roughness, and improving red blood streaks and acne.

[0026] Preferably, the preparation method of the Swertia indica extract is as follows:

[0027] (1) drying the Indian sedge sprouts, crushing them, and sieving them to obtain Indian sedge sprout powder; the mesh size of the Indian sedge sprout powder is 100-150 meshes;

[0028] (2) extracting the Indian sedge sprout powder with 15-30 times the amount of ethanol solution, concentrating it into a paste, and drying it to a constant weight to obtain the Indian sedge sprout extract;

[0029] Further preferably, the volume fraction of the ethanol solution is 70-90%; the extraction is ultrasonic extraction, the temperature of ultrasonic extraction is 30-40°C, the time of ultrasonic extraction is 0.5-1h, the power of ultrasonic extraction is 200-250W; the temperature of drying is 60-70°C.

[0030] Optionally, the preparation method of the Swertia indica extract is as follows:

[0031] (1) drying the Indian sedge sprouts, crushing them, and sieving them to obtain Indian sedge sprout powder; the mesh size of the Indian sedge sprout powder is 100-150 meshes;

[0032] (2) extracting the Indian sedge sprout powder with 15-30 times the amount of ethanol solution, collecting the supernatant and extracting with an organic solvent, concentrating the extract into a paste, and drying to a constant weight to obtain the Indian sedge sprout extract;

[0033] Preferably, the volume fraction of the ethanol solution is 70-90%; the extraction is ultrasonic extraction, the temperature of ultrasonic extraction is 30-40°C, the time of ultrasonic extraction is 0.5-1h, and the power of ultrasonic extraction is 200-250W; the organic solvent is at least one of chloroform, ethyl acetate, petroleum ether, and ethyl acetate; the drying temperature is 60-70°C.

[0034] The preparation method of the Indian Swertia extract will affect the performance of the final acne-repairing composition. The conventional preparation method of the Indian Swertia extract is to extract with an organic solvent after ultrasonic extraction. The method of extracting with an organic solvent after ultrasonic extraction may affect the proportion of active ingredients in the Indian Swertia extract. The active ingredients in the Indian Swertia extract finally extracted by different organic solvent extractants are not the same, and the proportion will also change greatly. The inventor found in the actual research process that in the preparation method of the Indian Swertia extract, the method of organic solvent extraction is not adopted. The Indian Swertia extract after ultrasonic extraction will be better synergistic with vitamin K2, guaiac extract, cerium oxide microspheres, and mulberry root extract. The obtained product has better effects of improving cell vitality, promoting blood circulation, and enhancing facial skin microcirculation, further reducing skin roughness, and improving red blood streaks and acne.

[0035] Preferably, the preparation method of the mulberry root extract is as follows:

[0036] (1) drying the mulberry root, crushing it, and sieving it to obtain mulberry root powder; the mesh size of the mulberry root powder is 100-150 mesh;

[0037] (2) extracting mulberry root powder with 20-60 times the amount of methanol solution, concentrating it into a paste, and drying it to constant weight to obtain the mulberry root extract;

[0038] Preferably, the volume fraction of the methanol solution is 70-90%; the extraction is flash extraction, the temperature of the flash extraction is 25-30°C, the time of the flash extraction is 50-80s, the voltage of the flash extraction is 150-250V, the speed of the flash extraction is 4000-5500r / min; the temperature of the drying is 40-50°C.

[0039] Further preferably, in step (2), 40-50 times the amount of methanol solution is used to extract the mulberry root powder.

[0040] The present invention provides a method for preparing the acne-removing and repairing composition, which is specifically as follows:

[0041] (1) Fully dissolving vitamin K2 in GTCC (a high-purity oil esterified with caprylic acid / capric acid and glycerol) to obtain an oil phase; the mass ratio of vitamin K2 to GTCC is 1:18-20;

[0042] (2) dissolving the guaiac extract, the Indian Swertia extract, the mulberry root extract, and the cerium oxide microspheres in deionized water according to weight parts to obtain an aqueous phase; wherein the weight ratio of the total amount of the guaiac extract, the Indian Swertia extract, the mulberry root extract, the cerium oxide microspheres and the deionized water is 1:5-8;

[0043] (3) Ultrasonic mixing the oil phase and the water phase in proportion to obtain the acne-repairing composition; the ratio of the oil phase to the water phase is 1:8-10.

[0044] In addition, the present invention provides the use of the acne-removing and repairing composition in the preparation of skin products.

[0045] Furthermore, the present invention provides a skin product, which includes the acne-removing and repairing composition.

[0046] Preferably, the skin product further comprises an auxiliary material, and the auxiliary material is at least one of a moisturizer, a thickener, a pH adjuster, an emulsifier, a preservative, a flavor, and deionized water.

[0047] Preferably, the skin product comprises one of lotion, emulsion, cream, mask, essence and spray.

[0048] Optionally, the present invention provides an emulsion comprising the following components in percentage by weight: 0.5-3% of acne-repairing composition, 1-20% of moisturizer, 0.05-0.5% of thickener, 0.05-0.1% of pH adjuster, 0.5-10% of emulsifier, 0.2-3% of preservative, 0.05-0.5% of fragrance, and the balance of deionized water.

[0049] Exemplarily, the moisturizer includes at least one of glycerin, butylene glycol, and low molecular weight sodium hyaluronate; the thickener includes at least one of xanthan gum, carbomer 980, and high molecular weight cellulose; the emulsifier includes at least one of coconut oil alcohol-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and parahydroxyacetophenone; the pH adjuster includes at least one of arginine and disodium EDTA.

[0050] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention provides an acne-repairing composition. The inventors found in the actual experimental process that when vitamin K2, Swertia indica extract, Guaiac extract, cerium oxide microspheres, and mulberry root extract are present at the same time, the five components can cooperate with each other and work together from multiple aspects to reduce skin roughness and improve red blood streaks and acne. Swertia indica extract, mulberry root extract, and guaiac extract work together to have anti-inflammatory and antibacterial properties, can inhibit the growth of acne pathogens such as Propionibacterium acnes, and reduce inflammatory reactions. The active ingredients in Swertia indica extract and mulberry root extract work together with guaiac extract to soothe and calm the skin, reduce vasodilation, promote local blood circulation, and relieve red blood streaks. The present application also adds vitamin K2 and cerium oxide microspheres, which can further protect vascular endothelial cells, enhance the elasticity and toughness of capillary walls, reduce capillary dilation, and enhance the resistance of capillary walls; at the same time, the addition of vitamin K2 and cerium oxide microspheres can further promote blood circulation, promote the regeneration and repair of skin cells, enhance skin barrier function, and improve skin roughness. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 The results of the acne removal test using the lotion of Application Example 1 on days D0 and D28 are shown in the figure;

[0052] Figure 2 The test results of removing red blood streaks on days D0 and D28 using the emulsion of Application Example 1 are shown in FIG.

[0053] Figure 3 The results of the acne removal test using the lotion of comparative application example 1 on days D0 and D28;

[0054] Figure 4 The results of the red blood streak removal test using the emulsion of comparative application example 1 on days D0 and D28. DETAILED DESCRIPTION

[0055] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0056] The raw materials used in the present invention are further described:

[0057] Vitamin K2: HPLC ≥ 98%, product number V140453, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.;

[0058] Vitamin K1: HPLC ≥ 98%, product number V820401, purchased from Shanghai MacLean Biochemical Technology Co., Ltd.;

[0059] Vitamin C: HPLC ≥ 98%, product number TC1384, purchased from Sichuan Jingcui Tiancheng Pharmaceutical Technology Co., Ltd.;

[0060] Guaiac wood extract: L2-DZ-SCB3675-X, purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.;

[0061] Cerium oxide microspheres: The trade name is luminescent TM , purchased from Huawei Technology (Beijing) Co., Ltd., with an average particle size of 200nm. The cerium oxide microspheres contain Ce 3+ , the Ce 3+ The molar proportion in the cerium oxide microspheres is 32%;

[0062] Ordinary cerium oxide-1: Product No. R005967, particle size 0.8-2 mm, purchased from Shanghai Roen Reagent Co., Ltd.;

[0063] Ordinary cerium oxide-2: Product No. C141507, particle size <5 μm, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.;

[0064] Mulberry leaf extract: Product No. S28490, purchased from Shanghai Yuanye Biotechnology Co., Ltd.;

[0065] Rosewood extract: Product No. TZ095, purchased from Xi'an Tongze Biotechnology Co., Ltd.;

[0066] Licorice extract: Product No. L860791, purchased from Shanghai MacLean Biochemical Technology Co., Ltd.;

[0067] Swertia indica Extract-1

[0068] The preparation method of the Swertia indica extract-1 comprises the following steps:

[0069] (1) drying the Indian sedge sprouts, crushing them, and sieving them to obtain Indian sedge sprout powder; the mesh size of the Indian sedge sprout powder is 120 meshes;

[0070] (2) Using 20 times the amount of ethanol solution to ultrasonically extract Indian musk deer sprout powder, after repeated extraction twice, passing through a 300-mesh sieve, collecting the filtrate, concentrating under reduced pressure to a paste, and drying to constant weight to obtain the Indian musk deer sprout extract; the volume fraction of the ethanol solution is 70%; the extraction temperature is 30°C, the extraction time is 1 hour, the extraction power is 250W; the drying temperature is 60°C.

[0071] Swertia indica Extract-2

[0072] The preparation method of the Swertia indica extract-2 comprises the following steps:

[0073] (1) drying the Indian sedge sprouts, crushing them, and sieving them to obtain Indian sedge sprout powder; the mesh size of the Indian sedge sprout powder is 150 mesh;

[0074] (2) Ultrasonic extraction of Indian musk deer sprout powder is performed using 30 times the amount of ethanol solution. After repeated extraction twice, the powder is passed through a 300-mesh sieve, the filtrate is collected, concentrated under reduced pressure to a paste, and dried to constant weight to obtain the Indian musk deer sprout extract; the volume fraction of the ethanol solution is 90%; the extraction temperature is 40° C., the extraction time is 0.5 h, the extraction power is 200 W; and the drying temperature is 70° C.

[0075] Swertia indica Extract-3

[0076] The preparation method of the Swertia indica extract-3, compared with the Swertia indica extract-1, only adds an organic solvent extraction process, and comprises the following steps:

[0077] (1) drying the Indian sedge sprouts, crushing them, and sieving them to obtain Indian sedge sprout powder; the mesh size of the Indian sedge sprout powder is 120 meshes;

[0078] (2) extracting the Indian sedge sprout powder with a 20-fold amount of ethanol solution, repeating the extraction twice, collecting the supernatant, extracting with an organic solvent, repeating the extraction three times, concentrating the extract under reduced pressure to a paste, and drying to a constant weight to obtain the Indian sedge sprout extract;

[0079] The volume fraction of the ethanol solution is 70%; the extraction is ultrasonic extraction, the temperature of ultrasonic extraction is 30° C., the time of ultrasonic extraction is 1 hour, and the power of ultrasonic extraction is 250W; the temperature of drying is 60° C.; and the organic solvent is chloroform.

[0080] Swertia indica Extract-4

[0081] The preparation method of the Swertia indica extract-4 is different from that of the Swertia indica extract-3, except that the organic solvent used for extraction is ethyl acetate, and the other components, weight parts and preparation method are exactly the same.

[0082] Swertia indica Extract-5

[0083] The preparation method of the Swertia indica extract-5 is different from that of the Swertia indica extract-3, except that the organic solvent used for extraction is petroleum ether, and the other components, weight parts and preparation method are exactly the same.

[0084] Mulberry root extract-1

[0085] The preparation method of the mulberry root extract-1 comprises the following steps:

[0086] (1) drying the mulberry root, crushing it, and sieving it to obtain mulberry root powder; the mesh size of the mulberry root powder is 100 mesh;

[0087] (2) Using 40 times the amount of methanol solution to extract mulberry root powder, repeat the extraction twice, pass through a 300-mesh sieve, collect the filtrate, reduce pressure and concentrate to a paste, and dry to constant weight to obtain the mulberry root extract; the volume fraction of the methanol solution is 75%; the extraction is a flash extraction, the flash extraction temperature is 30°C, the flash extraction time is 80s, the flash extraction voltage is 220V, the flash extraction speed is 4000r / min; the drying temperature is 40°C.

[0088] Mulberry Root Extract-2

[0089] The preparation method of the mulberry root extract-2 is compared with that of the mulberry root extract-1, except that in step (2), 50 times the amount of methanol solution is used to extract the mulberry root powder, and the other components, weight parts and preparation methods are exactly the same.

[0090] Mulberry Root Extract-3

[0091] The preparation method of the mulberry root extract-3 is different from that of the mulberry root extract-1, except that the volume fraction of the methanol solution in step (2) is 90%, and the other components, weight parts and preparation method are exactly the same.

[0092] Mulberry Root Extract-4

[0093] The preparation method of the mulberry root extract-4 is compared with that of the mulberry root extract-1, except that in step (2), 20 times the amount of methanol solution is used to extract the mulberry root powder, and the other components, weight parts and preparation methods are exactly the same.

[0094] Mulberry root extract-5

[0095] The preparation method of the mulberry root extract-5 is compared with that of the mulberry root extract-1, except that in step (2), 60 times the amount of methanol solution is used to extract the mulberry root powder, and the other components, weight parts and preparation methods are exactly the same.

[0096] Examples and Comparative Examples

[0097] The present application provides an acne-removing and repairing composition. The components and weight proportions of the acne-removing and repairing compositions of the embodiments and comparative examples are shown in Tables 1-1, 1-2 and 2 below.

[0098] The preparation method of the acne-removing and repairing composition is as follows:

[0099] Fully dissolve vitamin K2 in GTCC (a high-purity oil esterified from caprylic acid / capric acid and glycerol) to obtain an oil phase; the mass ratio of vitamin K2 to GTCC is 1:18;

[0100] Dissolve the guaiac extract, Swertia indica extract, Morus alba root extract, and cerium oxide microspheres in deionized water according to weight parts to obtain an aqueous phase; wherein the weight ratio of the total amount of the guaiac extract, Swertia indica extract, Morus alba root extract, cerium oxide microspheres and deionized water is 1:5;

[0101] The oil phase and the water phase are ultrasonically mixed in proportion to obtain the acne-repairing composition; the ratio of the oil phase to the water phase is 1:10.

[0102] When preparing the acne-repairing composition of the comparative example, if there is no relevant component, then no relevant component is needed. If there are other components, the components can be replaced according to the above preparation method to obtain the composition.

[0103] Table 1-1

[0104] Components (parts by weight) Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Vitamin K2 0.5 0.1 0.8 0.3 0.05 1 Swertia indica Extract-1 1 0.8 0.7 1.2 1.5 0.1 Guaiac Wood Extract 1.5 1.2 1 0.8 0.1 2 Cerium Oxide Microspheres 0.01 0.05 0.03 0.04 0.005 0.1 Mulberry root extract-1 4 2 3 4 5 0.5

[0105] Table 1-2

[0106]

[0107]

[0108] Table 2

[0109]

[0110]

[0111] Application Examples

[0112] Application Examples 1-15 of the present invention, Comparative Application Examples 1-14 and Blank Application Examples provide an emulsion, the components (mass percentage) of the emulsion are shown in Table 3;

[0113] The acne-repairing compositions used in Application Examples 1-14 are the acne-repairing compositions prepared in Examples 1-14, respectively, and the acne-repairing compositions used in Comparative Application Examples 1-14 are the acne-repairing compositions prepared in Comparative Examples 1-14, respectively; for example, the acne-repairing composition used in Application Example 1 is the acne-repairing composition prepared in Example 1, the acne-repairing composition used in Application Example 2 is the acne-repairing composition prepared in Example 2, the acne-repairing composition used in Comparative Application Example 1 is the acne-repairing composition prepared in Comparative Example 1, and the same can be said for other examples.

[0114] The acne-removing and repairing composition used in Application Example 15 is the acne-removing and repairing composition prepared in Example 1.

[0115] The method for preparing the emulsion of the present invention comprises the following steps:

[0116] (1) Mix phase A and water, heat to 80° C., and homogenize at 1200 rpm for 5 min. After homogenization, keep the mixture warm for later use to obtain prefabricated phase A;

[0117] (2) Mix phase B, heat to 80° C., and homogenize at 1200 rpm for 5 min. After homogenization, keep warm for later use to obtain prefabricated phase B;

[0118] (3) Mix phase C and heat to 60°C to melt to obtain prefabricated phase C;

[0119] (4) The preformed phase A is heated to 80° C., and the preformed phase B is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to 60° C., and the preformed phase C is added at a speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to below 45° C., and the acne-repairing composition and essence are added, and stirring is continued for 5 minutes. Finally, arginine is added to adjust the pH to 6.0, and stirring is stopped. The material is discharged to obtain an emulsion.

[0120] The preparation methods of the emulsions provided in the other application examples are consistent with that in application example 1, and no relevant components are added.

[0121] The selection of application example components and mass percentages are shown in Table 3 below.

[0122] Table 3

[0123]

[0124]

[0125] Performance Testing

[0126] Performance Test-1Cell Viability Test

[0127] Experimental materials: phosphate buffered saline (PBS), Shanghai Solebao; MTT, Yuanye Biotechnology; dimethyl sulfoxide (DMSO), Tianjin Damao; complete culture medium, MacLean; high glucose medium 1×DMEM, Gibco; fetal bovine serum (FBS), Gibco; keratinocytes were purchased from Guangzhou Customs Technology Center; human skin fibroblasts HSF were purchased from Kunming Cell Bank, Chinese Academy of Sciences.

[0128] Experimental steps: Inoculate the cell suspension into a 96-well cell culture plate and return to the incubator for 24 hours. Aspirate the complete culture medium in the well, add PBS, and expose to UVB light for 15 minutes (irradiation intensity: 80μW / cm 2 , irradiation dose: 80mJ / cm 2 );

[0129] The blank control group received complete culture medium without irradiation;

[0130] After irradiation for 15 minutes, the PBS solution was aspirated, and complete culture medium was added to the experimental group, and 100 μL of 0.5% acne repair composition was added to each well; only PBS was added to the model group without acne repair composition. The cells in each group were cultured for 24 hours. The culture plate was removed, 20 μL of MTT solution was added to each well, and incubated in the incubator for 3 hours. The liquid in the well was removed, 100 μL of DMSO was added to each well, and after being placed on an oscillator for 15 minutes, the absorbance OD value at a wavelength of 570 nm was measured on the microplate reader, and the cell viability was calculated.

[0131] All experiments were repeated at least three times according to the parallel design principle. The experimental data were expressed as mean ± SD. The cell viability was calculated as follows:

[0132] Cell viability = (OD value of sample well - OD value adjusted to zero) / (OD value of blank control well - OD value adjusted to zero) × 100%.

[0133] The test results are shown in Table 4.

[0134] Table 4

[0135]

[0136]

[0137] Note: When the t-test method is used for statistical analysis, the significance of the model group compared with the blank control group is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; the significance of the sample group compared with the blank control group is indicated by *, P-value < 0.05 is indicated by *, P-value < 0.01 is indicated by **, and P-value < 0.001 is indicated by ***.

[0138] The test results are shown in Table 4. The acne-repairing composition prepared in the examples provided in the present application can effectively promote the activity of keratinocytes in the epidermis and fibroblasts in the dermis under UVB irradiation damage.

[0139] Among them, the acne-removing and repairing composition in the present application optimizes the extraction conditions of the Indian Swertia extract and the mulberry root extract. The inventor found in the actual research process that in the preparation method of the Indian Swertia extract, the method of organic solvent extraction is not used. The Indian Swertia extract after ultrasonic extraction will be better synergized with vitamin K2, guaiac extract, cerium oxide microspheres, and mulberry root extract, and the obtained product has a better effect of improving cell viability. The inventor found in the actual research process that the mulberry root extract obtained by extracting mulberry root powder with 40-50 times the amount of methanol solution will be better synergized with vitamin K2, guaiac extract, cerium oxide microspheres, and mulberry root extract, and the obtained product has a better effect of improving cell viability.

[0140] The inventors found in the actual research process that vitamin K2 and cerium oxide microspheres can further protect vascular endothelial cells, enhance the elasticity and toughness of capillary walls, reduce capillary dilation, and enhance the resistance of capillary walls. The obtained product has a more excellent effect of improving cell viability. When vitamin K2, any one of Indian Swertia japonica extract, guaiacol extract, cerium oxide, and mulberry root extract is lacking, or any one of the active ingredients is lacking, and the weight of other ingredients is increased, the acne-repairing composition of comparative examples 1-7 obtained has a far less activating effect on skin cells than the embodiment, and the keratinocyte viability and fibroblast viability are significantly reduced. In addition, when other functionally similar components replace one of vitamin K2, Indian Swertia japonica extract, guaiacol, cerium oxide, and mulberry root extract, the effect of the comparative example obtained is also far less than the effect obtained by adopting the technical solution of the present application.

[0141] When the above components are within the scope of the present application, they have a relatively ideal synergistic effect.

[0142] Effect Example 2: ATP Test

[0143] Experimental content: Keratinocytes were cultured in vitro and incubated in DMEM medium containing or not containing the acne-repairing composition (the acne-repairing composition was diluted to 0.005% using the culture medium solution), and the blank control group was used without the acne-repairing composition. The cells were cultured for 48 hours, and the ATP content in the cells was tested using a commercially available kit (item number S0026, purchased from Beyotime) at 24 hours and 48 hours, respectively. The intracellular ATP content was determined by bioluminescence, and 0.01% cytochrome C was used as the positive control group. The relative ATP content was calculated as follows:

[0144] Relative ATP content (% blank control group) = ATP content experimental group / ATP content blank control group * 100%. The experimental results are shown in Table 5:

[0145] Table 5

[0146]

[0147]

[0148] Note: When the t-test method is used for statistical analysis, compared with the blank control group, the significance is indicated by *, P-value < 0.05 is indicated by *, P-value < 0.01 is indicated by **, and P-value < 0.001 is indicated by ***.

[0149] It can be seen from the above table that the acne-removing and repairing composition prepared in the examples provided in this application has a higher relative ATP content.

[0150] Among them, the acne-removing and repairing composition in the present application optimizes the extraction conditions of the Indian Swertia extract and the mulberry root extract. The inventor found in the actual research process that in the preparation method of the Indian Swertia extract, the method of organic solvent extraction is not used. The Indian Swertia extract after ultrasonic extraction will be better synergized with vitamin K2, guaiac extract, cerium oxide microspheres, and mulberry root extract, and the obtained product has a better effect of increasing the relative ATP content. The inventor found in the actual research process that the mulberry root extract obtained by extracting mulberry root powder with 40-50 times the amount of methanol solution will be better synergized with vitamin K2, guaiac extract, cerium oxide microspheres, and mulberry root extract, and the obtained product has a better effect of increasing the relative ATP content.

[0151] The inventors found in the actual research process that vitamin K2 and cerium oxide microspheres can further protect vascular endothelial cells, enhance the elasticity and toughness of capillary walls, reduce capillary dilation, and enhance the resistance of capillary walls. The resulting product has a better effect of increasing the relative ATP content.

[0152] When any one of vitamin K2, Swertia indica extract, Guaiacol, cerium oxide, and mulberry root extract is lacking, or any one of the active ingredients is lacking, and the weight of other ingredients is increased, the improvement of the relative ATP content of the acne-repairing composition of comparative examples 1-7 is far less than that of the embodiment. Moreover, when other components with similar functions replace one of vitamin K2, Swertia indica extract, Guaiacol, cerium oxide, and mulberry root extract, the effect of the comparative example obtained is also far less than that obtained by using the technical solution of the present application.

[0153] When the above components are within the scope of the present application, they have a relatively ideal synergistic effect.

[0154] Effect Example 3: Blood Flow Test

[0155] Zebrafish embryos are transparent and have become a classic research model for human cardiovascular research. Under a microscope, the flow of blood cells in blood vessels is clearly visible, so zebrafish embryos are very suitable for testing the blood circulation-promoting effects of samples.

[0156] (1) Zebrafish source and breeding: Use wild-type AB strain zebrafish (Danio rerio) from a reliable source (China National Zebrafish Resource Center) for spawning tests. Broodstock should have good reproductive ability - the best age is 6-12 months. Cross-crossing should be used as much as possible to maintain genetic diversity. A new batch of broodstock should be replaced after 5 generations of pure strain broodstock. Broodstock should not have obvious visible signs of infection and disease, and should not have undergone drug treatment within 2 months. Broodstock should be domesticated for more than 14 days after being introduced into the laboratory before spawning tests.

[0157] (2) Test grouping: Select healthy zebrafish embryos that are 3 days old after fertilization. The test needs to set up a blank control group (fish embryo culture medium / solvent solution) and a test substance group (test substance).

[0158] 40 fish embryos were randomly selected, transferred to a 6 cm culture dish, and 8 mL of fish embryo culture medium was added.

[0159] 40 fish embryos were randomly selected and transferred to a 6 cm culture dish, and 8 mL of 0.05 g / L caffeine solution was added (the working solution was 0.05 g / L caffeine solution prepared with fish embryo culture medium).

[0160] 40 fish embryos were randomly selected and transferred to a 6 cm culture dish, and 8 mL of the test substance solution (the test substance was prepared as a 0.1% acne-repairing composition using dimethyl sulfoxide as solvent) was added.

[0161] Each group was placed in a 28±1℃ constant temperature incubator and cultured for 2±0.5h.

[0162] The fish embryos were anesthetized for 2 min with tricaine working solution (200 mg tricaine (analytical grade) was weighed and dissolved in 48.95 mL water to prepare the storage solution, which was refrigerated and valid for 30 days. The storage solution was diluted to 0.04% with fish embryo culture medium and freshly prepared before use). The dorsal aorta of the fish embryo was photographed for 20 s under a microscope.

[0163] (3) Data and result calculation: Use software such as DanioScope to measure the relative velocity of blood flow in the dorsal aorta of each fish embryo and take the average value.

[0164] Calculate the blood circulation promotion rate: promotion rate = (TC) / C×100%;

[0165] Where: T is the average relative velocity of blood flow in the dorsal aorta of fish embryos in the test substance treatment group; C is the average relative velocity of blood flow in the dorsal aorta of fish embryos in the blank control group;

[0166] Table 6

[0167]

[0168]

[0169] Note: When the t-test method is used for statistical analysis, the significance of the model group compared with the blank control group is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; the significance of the sample group compared with the blank control group is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0170] It can be seen from the above table that the acne-repairing composition prepared in the examples provided in this application has a better effect in promoting blood circulation.

[0171] Among them, the acne-removing and repairing composition in the present application optimizes the extraction conditions of the Indian Swertia extract and the mulberry root extract. The inventor found in the actual research process that in the preparation method of the Indian Swertia extract, the method of organic solvent extraction is not used. The Indian Swertia extract after ultrasonic extraction will be better synergized with vitamin K2, guaiac extract, cerium oxide microspheres, and mulberry root extract, and the obtained product has a better effect of promoting blood circulation. The inventor found in the actual research process that the mulberry root extract obtained by extracting mulberry root powder with 40-50 times the amount of methanol solution will be better synergized with vitamin K2, guaiac extract, cerium oxide microspheres, and mulberry root extract, and the obtained product has a better effect of promoting blood circulation.

[0172] The inventors found in the actual research process that vitamin K2 and cerium oxide microspheres can further protect vascular endothelial cells, enhance the elasticity and toughness of capillary walls, reduce capillary dilation, and enhance the resistance of capillary walls. The resulting product has a more excellent effect in promoting blood circulation.

[0173] When any one of vitamin K2, Swertia indica extract, Guaiac extract, cerium oxide, and mulberry root extract is lacking, or any one of the active ingredients is lacking, and the weight of other ingredients is increased, the acne-repairing compositions of comparative examples 1-7 obtained have a far less effect on promoting blood circulation than the embodiments. Moreover, when other components with similar functions replace one of vitamin K2, Swertia indica extract, Guaiac extract, cerium oxide, and mulberry root extract, the effect of the comparative examples obtained is also far less than the effect obtained by using the technical solution of the present application.

[0174] When the above components are within the scope of the present application, they have a relatively ideal synergistic effect.

[0175] Effect Example 4

[0176] The effect example of the present invention verifies the safety of the application example on human skin. According to the human skin patch test "Safety Technical Specifications for Cosmetics" (2015), 33 volunteers aged 18-60 were recruited for the test, and the test environment was temperature (21±1)℃ and humidity (50±10)%. The volunteers washed their forearms with clean water, and after 5-10 minutes, after the moisture in the forearms evaporated, the testers applied 0.020-0.025mL of each group of sample emulsions to each chamber of the 10-hole patch tester, and selected the inner side of the two forearms of the volunteers, avoiding the skin at the front and back hand joints. Each arm was attached with a sample patch tester, and the corresponding marks were made. The patch time lasted for 24 hours, and the patch tester was removed and the residual sample was wiped. The skin reaction of 0.5h, 24h, and 48h was observed, and the results were recorded according to the skin reaction grading standard in the "Safety Technical Specifications for Cosmetics" (2015). As shown in Table 7, the skin reaction is divided into 4 scoring levels, and the higher the score, the higher the severity of the skin irritation reaction.

[0177] The test results are as follows: 0 cases of withdrawal and 0 cases of adhesive tape allergy in the technical solution provided by the present invention. Among them, no adverse reactions occurred in the application examples, comparative application examples and blank application examples.

[0178] Table 7

[0179]

[0180]

[0181] Effect Example 5 Human Efficacy Test

[0182] This effect example tests the improvement effects of application examples 1-15, comparative application examples 1-14 and blank application examples on human skin TEWL, moisturizing, roughness, acne counts and red blood streaks.

[0183] The specific test method is as follows: According to the "Technical Specification for Safety of Cosmetics" (2015), volunteers with sensitive skin aged 18-50 (lactic acid stinging score > 3) were selected and randomly divided into 30 groups, with 8 people in each group. The volunteers had obvious acne, blackheads, and papules. Under normal circumstances, the volunteers continued to use the product on their entire face for 28 days; every morning and evening after cleansing and moisturizing, the volunteers applied an appropriate amount of lotion on their face and massaged for absorption. The acne area can be overlapped and the entire face is used once in the morning and evening, with follow-up visits on the 1st, 3rd, 7th, 14th, and 28th days.

[0184] On the day of the volunteer visit, the volunteers washed their faces with water in the test area without applying any products, and sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. The improvement of facial acne indicators was quantified and photographed using instruments, including acne counts (classification counts: comedones, papules, respectively evaluated by VISIA and doctors), Tewameter TM Hex test for transepidermal water loss TEWL, Corneometer CM 825 test for moisturizing, VC 20Plus test for roughness, and MX 18 test for red blood streaks.

[0185] Improvement rate before and after use = |X mean value before use - X mean value after use | / X mean value before use × 100%; the calculation results are shown in Table 8. The improvement rate presented in Table 8 is the improvement rate of the follow-up visit on the 28th day.

[0186] Table 8

[0187]

[0188]

[0189]

[0190]

[0191] Note: * indicates that the difference is statistically significant compared with the blank application example (P < 0.05).

[0192] As can be seen from the above table, the acne-repairing composition prepared in the examples provided in this application has excellent effects of improving facial skin microcirculation, further reducing skin roughness, and improving red blood streaks and acne after being prepared into an emulsion. Figure 1 This is the result of the acne removal test using the lotion in Application Example 1 on days D0 and D28. Figure 2 This is the test result of removing red blood streaks on days D0 and D7 using the lotion in Application Example 1. Figure 3 The results of the acne removal test using the lotion in comparative application example 1 on days D0 and D28 are shown in the figure. Figure 4 The results of the red blood streak removal test using the emulsion in comparative application example 1 on days D0 and D28. Figure 1-4By comparison, it can be seen that the emulsion of Application Example 1 of the present invention has a very obvious effect in improving red blood streaks and acne.

[0193] Among them, the acne-removing and repairing composition in the present application optimizes the extraction conditions of the Indian Swertia extract and the mulberry root extract. The inventor found in the actual research process that in the preparation method of the Indian Swertia extract, the method of organic solvent extraction is not used. The Indian Swertia extract after ultrasonic extraction will be better synergized with vitamin K2, guaiac extract, cerium oxide microspheres, and mulberry root extract. The obtained product has a better effect of improving facial skin microcirculation, further reducing skin roughness, and improving red blood streaks and acne. The inventor found in the actual research process that the mulberry root extract obtained by extracting mulberry root powder with 40-50 times the amount of methanol solution will be better synergized with vitamin K2, guaiac extract, cerium oxide microspheres, and mulberry root extract. The obtained product has a better effect of improving facial skin microcirculation, further reducing skin roughness, and improving red blood streaks and acne.

[0194] The inventors discovered during the actual research process that vitamin K2 and cerium oxide microspheres can further protect vascular endothelial cells, enhance the elasticity and toughness of capillary walls, reduce capillary dilation, and enhance the resistance of capillary walls. The resulting product has a better effect of improving facial skin microcirculation, further reducing skin roughness, and improving red blood streaks and acne.

[0195] When any one of vitamin K2, Swertia japonica extract, Guaiac wood extract, cerium oxide, and Morus alba extract is lacking, or any one of the active ingredients is lacking, and the weight of other ingredients is increased, the acne-removing and repairing compositions of Comparative Examples 1-7 obtained are far less effective than the embodiments in improving facial skin microcirculation, reducing skin roughness, and improving red blood streaks and acne.

[0196] Moreover, when other components with similar functions replace one of vitamin K2, Swertia indica extract, guaiacol, cerium oxide, and mulberry root extract, the effect of the comparative example obtained is far inferior to the effect obtained by adopting the technical solution of the present application.

[0197] When the above components are within the scope of the present application, they have a relatively ideal synergistic effect.

[0198] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An acne-removing and repairing composition, characterized in that: The invention comprises the following components in parts by weight: 0.05-1 part of vitamin K2, 0.1-1.5 parts of Swertia indica extract, 0.1-2 parts of Guaiac wood extract, 0.005-0.1 parts of cerium oxide microspheres, and 0.5-5 parts of mulberry root extract; wherein the average particle size of the cerium oxide microspheres is 0.2-3 μm, and the cerium oxide microspheres contain Ce 3+ , the Ce 3+ The molar proportion in the cerium oxide microspheres is 30-38%.

2. The acne-removing and repairing composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 0.1-0.8 parts of vitamin K2, 0.6-1.2 parts of Swertia indica extract, 0.8-1.5 parts of Guaiac wood extract, 0.01-0.05 parts of cerium oxide microspheres and 2-4 parts of mulberry root extract.

3. The acne-removing and repairing composition according to claim 1, characterized in that: In the acne-removing and repairing composition, the total mass percentage of vitamin K2 and cerium oxide microspheres is 2-10%; And / or, in the acne-repairing composition, the mass percentage of Swertia indica extract is 10-20%; And / or, in the acne-repairing composition, the mass percentage of mulberry root extract is 40-60%.

4. The acne-removing and repairing composition according to claim 1, characterized in that: The preparation method of the Swertia indica extract is as follows: (1) drying the Indian sedge sprouts, crushing them, and sieving them to obtain Indian sedge sprout powder; the mesh size of the Indian sedge sprout powder is 100-150 meshes; (2) Using 15-30 times the amount of ethanol solution to extract the Indian sedge sprout powder, concentrating it into a paste, and drying it to a constant weight to obtain the Indian sedge sprout extract.

5. The acne-removing and repairing composition according to claim 4, characterized in that: In the step (2), the volume fraction of the ethanol solution is 70-90%; the extraction is ultrasonic extraction, the temperature of ultrasonic extraction is 30-40°C, the time of ultrasonic extraction is 0.5-1h, the power of ultrasonic extraction is 200-250W; the temperature of drying is 60-70°C.

6. The acne-removing and repairing composition according to claim 1, characterized in that: The preparation method of the mulberry root extract is as follows: (1) drying the mulberry root, crushing it, and sieving it to obtain mulberry root powder; the mesh size of the mulberry root powder is 100-150 mesh; (2) Using 20-60 times the amount of methanol solution to extract mulberry root powder, concentrating it into a paste, and drying it to constant weight to obtain the mulberry root extract.

7. The acne-repairing composition according to claim 6, characterized in that: In the step (2), the volume fraction of the methanol solution is 70-90%; the extraction is a flash extraction, the temperature of the flash extraction is 25-30°C, the time of the flash extraction is 50-80s, the voltage of the flash extraction is 150-250V, the speed of the flash extraction is 4000-5500r / min; the temperature of the drying is 40-50°C.

8. Use of the acne-repairing composition according to any one of claims 1 to 7 in the preparation of skin products.

9. A skin care product, characterized in that: The skin product comprises the acne-repairing composition according to any one of claims 1 to 7.

10. The skin care product according to claim 9, wherein: The skin products include one of lotion, emulsion, cream, facial mask, essence and spray.

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