40 results about "Vitamin B12" patented technology

The application provides a biological analysis method capable of accurately, simply and quickly detecting the concentration of adenosylcobalamin in human serum. The method is a liquid chromatography tandem mass spectrometry method, octadecylsilane bonded silica gel is used as the column filler, the liquid phase conditions, mass spectrometry parameters and sample processing method are designed and optimized, and the application first proposes and discloses a biological analysis method capable of accurately and quickly detecting the concentration of adenosylcobalamin in human serum: after simple treatment (need to avoid light) such as protein precipitation and nitrogen blowing dry, the sample can be injected, the analysis time is only 4.00 min, the lower limit of quantification is as low as 5 pg / mL, and the sample dosage is only 100 muL. The application can quickly monitor the change of vitamin B12 in the body, meet the requirements of adenosylcobalamin in vivo drug monitoring, pharmacokinetics and bioequivalence, and can effectively improve the safety and reliability of the drug.

This invention provides a method for producing vitamin B12 using microchannel reaction technology. The method involves acidifying, hydrolyzing, filtering, and analyzing the vitamin B12 fermentation broth, followed by a cyanidation reaction in a microchannel reactor. Further filtration, analysis, and concentration yield vitamin B12. This method produces cyanide-treated vitamin B12 with a thorough conversion reaction and increased yield. It also reduces the amount of cyanide used, thus reducing pollution and costs. The equipment has good airtightness, and the small amount of hydrogen cyanide gas generated is completely recovered and centrally treated, greatly reducing harm to the environment and human health. This method is environmentally friendly and contributes to green production.

This invention provides a method for improving the filtration efficiency of vitamin B12 fermentation broth. First, the secondary filtrate after plate and frame filtration is adsorbed onto a resin column and elute with ammonia. Then, the eluent is filtered through a molecular sieve, and the vitamin B12 content in the filtrate is determined using high-performance liquid chromatography (HPLC). This preparation method effectively removes small molecule impurities such as ammonia from the 50-column eluent, alters the alkaline environment of the filtrate to prevent vitamin B12 degradation, reduces impurity content, and improves the quality of vitamin B12.

This invention relates to a mouthwash for treating oral ulcers and its preparation method. The mouthwash comprises the following components in the indicated weight ratios: 100 parts deionized water, containing 5-35 parts sodium chloride, 1-10 parts sodium bicarbonate, 0.1-1 parts borneol, 0.1-1 parts peppermint oil, 1-5 parts vitamin E, and 1-5 parts vitamin B12. First, all the above materials (excluding deionized water) are mixed in the indicated weight ratio and ground into a fine powder finer than 400 mesh. Then, peppermint oil is sprayed onto the powder mixture and stirred evenly to obtain the prepared mouthwash powder. The powder is then packaged and sealed. To use, the mouthwash powder is poured into a bottle containing the corresponding weight of deionized water and shaken until fully dissolved before use. This mouthwash has significant effects in treating oral ulcers, providing timely pain relief, accelerating ulcer healing, and shortening the course of the disease.

The present application relates to the technical field of nutritional supplement food, more specifically, to a double-protein balanced type of old-age nutritional supplement food and a preparation method thereof. The food comprises calcium, magnesium, zinc, iron, vitamin C, vitamin B6, vitamin E, vitamin B2, sodium selenite, nicotinamide, vitamin D3, vitamin A, vitamin B12, folic acid, vitamin B1 and vitamin K2. The present application is targeted at physiological characteristics and metabolic rules of middle-aged and old people, and scientifically proportioned various vitamins, minerals, essential fatty acids and antioxidant substances required by human body, so as to fully meet daily nutritional requirements of the middle-aged and old people and make up for insufficient dietary intake caused by decreased digestive and absorptive functions.

This invention discloses a microsphere-oil gel composite sustained-release injection and its preparation method. The method includes the following steps: dissolving PVA in pure water to obtain an aqueous phase; dissolving PLGA in an organic solvent to form an organic phase; adding vitamin B12 in solid form to the organic phase to form a solid-oil phase suspension system; adding the solid-oil phase suspension system to the aqueous phase for emulsification to form a solid-oil-aqueous emulsion; adding the emulsion to excess pure water to form drug-loaded PLGA microspheres and freeze-drying; using peanut oil as the oil phase, heating to 60-65°C, adding a gelling agent (a mixture of glyceryl monostearate and beeswax) to the oil phase to form a clear and homogeneous oil phase system; cooling the oil phase system to 30-40°C, adding drug-loaded PLGA microspheres, and uniformly dispersing them under stirring conditions, then continuing to cool naturally to room temperature to obtain the microsphere-oil gel composite sustained-release injection.

The application discloses a composition containing burdock polysaccharide for reducing blood sugar of type 2 diabetes patients and a preparation method and application thereof, and belongs to the technical field of medicine and food. The composition contains burdock polysaccharide, chitooligosaccharide, chlorogenic acid, epicatechin, resveratrol, ginsenoside Rd, and vitamin D, vitamin B1, vitamin B6, vitamin B12, magnesium glycinate and thaumatin protein. The application takes burdock polysaccharide as a core long-acting component, takes chitooligosaccharide as a short-acting synergistic component, constructs a long-short-acting synergistic blood sugar reducing system, and realizes the organic combination of rapid blood sugar reduction and stable blood sugar control. Experiments show that the composition can significantly reduce the fasting blood sugar of type 2 diabetes model rats, improve insulin resistance and glucose tolerance, reduce blood lipid and inflammatory factor levels, and reduce oxidative stress damage. The alpha-glucosidase inhibitory activity and in-vivo comprehensive blood sugar reducing effect of the composition are better than those of single burdock polysaccharide, and the composition has good taste and is suitable for long-term use of diabetes patients.

This invention, entitled "A Sample Pretreatment Solution, Pretreatment Method, and Application for Vitamin B12 Detection," belongs to the field of analytical detection technology. The technical problem to be solved is how to simultaneously improve the accuracy and safety of vitamin B12 detection. The key technical solution is a sample pretreatment solution for vitamin B12 detection, comprising: 10 g / L–30 g / L of alkali metal hydroxide, 0.005 g / L–0.05 g / L of cyanide, and 0.5 g / L–1.5 g / L of disodium ethylenediaminetetraacetate (EDTA). By adding disodium EDTA, the alkali metal hydroxide, cyanide, and disodium EDTA work synergistically to effectively reduce the concentration of highly toxic cyanide, which requires strict safety management, thereby simultaneously improving the overall accuracy and operational safety of vitamin B12 detection.

A composition for improving cognitive performance, enhancing mental clarity, increasing focus, and increasing physical and mental energy is disclosed. The composition includes sufficient amounts of Fermented Green Tea Extract, L Theanine, and Caffeine, Ashwagandha Extract, Black Pepper Extract, Huperzine, Fermented Alpha GPC / Citoline Matrix, Turmeric Root Extract, Lions Mane Extract, Ginkgo Biloba Powder Extract, Panax Ginseng Extract, Rhodiola Extract, Cognitive Probiotic Blend, Folic Acid, Vitamin D3, Vitamin C Powder, Vitamin B12, Vitamin B6, Pantothenic Acid, Vitamin B3 Crystalline Powder, Vitamin B2 Powder, Vitamin B1 HCL, and Biotin.

This invention provides a composition containing vitamins and minerals, comprising vitamin D, folic acid, vitamin B12, potassium, and excipients in specific proportions. The invention may also include vitamin B1 and vitamin B2. The components of this composition have a synergistic effect in preventing hypertension, particularly in preventing hypertension accompanied by elevated homocysteine ​​levels. Furthermore, this composition can also be used for targeted nutritional supplementation in specific populations.

The present disclosure provides compositions and methods for providing a health benefit to a growing animal comprising administering a food composition to the animal, wherein the food composition includes a brain development blend. The brain development blend generally comprises DHA, EPA, taurine, choline, thiamine, riboflavin, pantothenic acid, niacin, pyridoxine, vitamin B12, and folic acid.

The present application relates to a kind of drug composition for preventing and adjuvant therapy of Alzheimer's disease and preparation method thereof, belong to medical health technical field.The prescription of the drug composition prepared in the present application includes the following active ingredients, phosphatidylserine, pyrrole quinoline quinone, coenzyme Q10, curcumin, EGCG, alpha-lipoic acid, acetyl L-carnitine, magnesium threonate, vitamin E, folic acid and vitamin B12.The preparation method of the drug composition also includes the following steps: the product is loaded with energy by weak magnetic resonance equipment to the composition.The drug composition reaches the effect of antioxidation, anti-inflammation, improves cerebral blood flow and protects neuron by the synergistic effect of multiple functional components, so as to help slow down the progress of Alzheimer's disease.

The application provides a kind of granule conveying device for vitamin B12 food additive, relating to food additive processing technical field, including raw material tank, discharge pipeline is vertically connected to the lower end of raw material tank, discharge pipeline includes discharge section, anti-sticking section and feeding section from top to bottom, the pipe diameter of discharge section and the pipe diameter of feeding section are all greater than the pipe diameter of anti-sticking section;Cooling sleeve is sleeved on the outer periphery of discharge pipeline and covers anti-sticking section, cooling space is formed between cooling sleeve and discharge pipeline, the gas inlet and gas outlet for guiding cooling gas into and out of cooling space are arranged on the peripheral wall of cooling sleeve, to cool the raw materials flowing through anti-sticking section, avoid the oxidation and deterioration problem caused by long-term residence of raw materials adhering to pipe wall in high temperature environment in traditional way.

The application discloses a lemon plant health beverage which is composed of lemon, vitamin C, vitamin B1, vitamin B2, vitamin B6, vitamin B9, vitamin B12, honey and mineral water. The lemon health beverage does not add any chemical additives, preservatives, pigments and preservatives, is very safe and healthy. And the beverage can supplement vitamin C and various B vitamins for the human body according to the WHO standard and the required vitamin intake per day of adults, can help to improve immunity, promote digestion, maintain nerve and cardiovascular health, and has the effects of antioxidation, anti-inflammation, auxiliary disease risk reduction and the like.

This invention discloses a composition for improving microcirculation in the pet's brain and nourishing nerves, its preparation method, and its application. Belonging to the field of pet medical technology, it specifically relates to a composition for improving microcirculation in the pet's brain and nourishing nerves, comprising: 2-5 parts of Ginkgo biloba extract, 10-20 parts of curcumin, 5-10 parts of α-lipoic acid, 75-125 parts of Astragalus polysaccharide, 0.5-5 parts of vitamin B12, and 0.25-2 parts of vitamin B1. This invention scientifically combines Ginkgo biloba extract, curcumin, α-lipoic acid, Astragalus polysaccharide, vitamin B1, and vitamin B12, utilizing the synergistic effects of each component in anti-inflammatory, antioxidant, microcirculation improvement, and neurotrophic effects, to provide a composition that can effectively improve microcirculation in the pet's brain and nourish nerves, filling a gap in the prior art.

The application provides a method for supplementing nutrients required for growth of a vitamin B12 fermentation production strain, which comprises the following steps: in a fermentation operation stage, according to consumption, supplementing carbon source glucose to maintain basic metabolism, and supplementing nutrients after operation for 90 hours. The feeding method provided by the application can improve the activity of the vitamin B12 fermentation production strain, effectively prolongs the stable period of the strain, prolongs the fermentation cycle by 7.46%, and has obvious effect on the improvement of the fermentation titer, and the improvement ratio is 8.30%.

This invention relates to a method for the non-toxic preparation of vitamin B12, comprising the following steps: first, under antioxidant conditions, the fermentation broth of *Pseudomonas denitrificans* is converted using a cyanide ion donor as a conversion agent; then, using polyaluminum ferric chloride as a precipitant, the vitamin B12 conversion broth is precipitated, and solid-liquid separation is performed to obtain vitamin B12. This invention effectively improves the conversion rate of vitamin B12 and removes cyanide residues through the synergistic effect of the conversion and precipitation reactions under antioxidant conditions.

The present invention relates to orally administrated compositions suitable for supporting women with premenstrual syndrome, which compositions contain: a) stereoisomers of inositol that contribute to the normal function of the ovaries, specifically myoinositol and D-chiro-inositol in a ratio of myoinositol: D-chiro-inositol from 2:1 to 5:1; b) vitamins of the B complex that contribute to normalpsychological function, preferably folic acid and / or vitamin B2 and / or vitamin B6 and / or vitamin B12; c) osier extract and / or Rooibos extract that contribute in normal psychological function; d) N-acetylcysteine and e) one or more inactive excipients.

FIELD: food industry.SUBSTANCE: intended for preventive nutrition of individuals experiencing increased psycho-emotional stress. A drink concentrate is proposed to reduce psycho-emotional stress and maintain cognitive stability, which is made on the basis of biologically active components in the form of amino acids L-theanine, taurine and glycine, vitamins of group B-B1, B6 and B12, magnesium citrate, plant extracts of lemon balm and rose hips, β-glucan, ascorbic and succinic acids, stevia sweetener and water, and is made in two phases by combining the aqueous and lipophilic phases and homogenizing the prepared components, while the amino acids L-theanine, taurine and glycine are used in microencapsulated form with a polysaccharide shell based on maltodextrin and sodium alginate and with B vitamins and magnesium citrate are introduced during the preparation of the aqueous phase, and plant extracts of lemon balm and rose hips and β-glucan – in the preparation of a lipophilic phase stabilized with plant emulsifiers, while the total content of biologically active components per 20 ml of concentrate is selected within the range: L-theanine – 100–200 mg, taurine – 400–600 mg, glycine – 600–1000 mg, magnesium citrate – 300–500 mg, vitamin B1 – 1.5–2.5 mg, vitamin B6 – 1.5–2.5 mg, vitamin B12 – 0.001–0.003 mg, lemon balm herbal extract – 200–400 mg, rosehip herbal extract – 200–300 mg, ascorbic acid – 80–120 mg, succinic acid – 100–200 mg, β-glucan – 0.1–0.5 wt.%, stevia sweetener – 10–30 mg. In this case, before introducing biologically active components, the water is subjected to ultrasonic treatment at a frequency of 20–40 kHz for 3–5 minutes.EFFECT: increasing the bioavailability and stability of active substances, prolonged physiological action and reducing the effects of psycho-emotional stress.2 cl, 3 ex

The present disclosure relates to an oral product, a process for producing the oral product, as well as to uses of said oral product. The oral product comprises a combination of active ingredients, wherein the combination comprises: caffeine; a combination of at least two B vitamins selected from vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B7, vitamin B9, and vitamin B12; and beta-alanine.

A nasal spray solution for hangovers and method for its preparation. The nasal spray solution may be used to alleviate headache, fatigue, nausea, redness of the eyes, raised blood pressure, congestion, dizziness and other symptoms resulting from the consumption of alcohol. The main active components of the nasal spray solution are vitamin B12, aloe vera extract, L-glutamine, and potassium chloride.

The present application relates to the production process of vitamin B12, the process steps include pretreatment, ceramic membrane filtration, adsorption, adding hydrocyanic acid through pipeline reactor cyanidation, concentration, column chromatography and crystallization drying. The purpose of the present application is to optimize the extraction and purification process of vitamin B12, and provide a method with safe process steps, high extraction yield and product quality meeting the quality standards of foreign customers.

The invention provides a method for improving the cyanidation conversion rate of vitamin B12, which comprises the following steps: immobilizing a catalyst nickel on a carrier graphene, catalyzing the conversion efficiency of the cyanidation vitamin B12 by using an immobilized catalyst technology, controlling the dosage of the catalyst, regulating the pH value of a reaction solution, and controlling the reaction time and reaction temperature, thereby obtaining the cyanidation conversion rate of the vitamin B12. The cyanidation conversion rate of the fermentation liquor is averagely increased by 13.47%, the total impurity content is integrally reduced to 8.8% or below, the catalyst can be recycled through simple operation, the subsequent purification process of the product is reduced, and the method has higher economic benefits.

This invention discloses a genetically engineered *Streptococcus adhesioides* strain that enhances vitamin B12 production, belonging to the field of bacterial engineering technology. The genetically engineered strain contains a recombinant expression plasmid containing the natural *cobPWNO* operon. The *cobPWNO* operon consists of the natural promoter of the *cobP* gene and the coding sequences of the *cobP*, *cobW*, *cobN*, and *cobO* genes in tandem. This invention, by preserving the natural operon structure through overexpression, perfectly maintains the inherent synergistic regulatory relationship between the four key genes. This coordinated overexpression method ensures a balanced enzyme ratio in each step of the metabolic pathway, allowing for smooth metabolic flow. This not only enhances the synthesis of the target product, vitamin B12, but also avoids the accumulation of intermediate products or metabolic disorders caused by excessively strong local metabolic nodes. Furthermore, by utilizing the gene's own natural promoter, it avoids cellular stress responses that may be triggered by strong exogenous promoters, ensuring normal bacterial growth.

The application relates to the technical field of food detection, in particular to a special medical food vitamin B12 enrichment and detection material and method based on magnetic molecular imprinting. The material is a magnetic molecular imprinting polymer (MMIPs), which takes Fe3O4 as a magnetic core, is coated with a SiO2 layer and a KH570 coupling agent in sequence, and is coupled with a vitamin B12 imprinting polymer layer through surface molecular imprinting technology. The imprinting polymer layer is polymerized from a template molecule vitamin B12 (CNcbl), a functional monomer methacrylic acid (MAA) and a crosslinking agent trimethylolpropane trimethacrylate (TRIM). The material of the application realizes specific recognition through molecular imprinting technology, and simplifies a separation step by using superparamagnetism, so that the cost is significantly reduced and the efficiency is improved.

The present application relates to a kind of finished product vitamin B12 cyanide ion detection method, its steps include dissolution, adsorption and resolution, concentration and titration method detection.The present application provides a kind of simple process step, remove the color influence of finished product vitamin B12, ensure that cyanide ion detection result is correct method.

This invention relates to a method for extracting vitamin B12 from vitamin B12 fermentation broth, comprising the steps of pretreatment, ceramic membrane filtration, adsorption, addition of thiocyanate for cyanation via a microreactor, concentration, column chromatography, and crystallization drying. The purpose of this invention is to optimize the vitamin B12 extraction and purification process, providing a method with safe process steps, high extraction yield, and finished product quality meeting the standards of overseas customers.

This application relates to a large-particle dog and cat oral health functional food coated with quick-dissolving freeze-dried granules and its preparation method, belonging to the field of pet food technology. This formulation contains grain-based granules, an external spraying system consisting of 0.1-0.15 parts lecithin, 0.002-0.003 parts monoglycerides, 0.001-0.003 parts vitamin E, and 0.8-1.2 parts chicken fat, and an instant-dissolving freeze-dried microparticle layer consisting of 0.1-0.2 parts brown algae polysaccharide, 0.02-0.05 parts lysozyme, 0.04-0.08 parts tea polyphenols, 0.02-0.04 parts zinc gluconate, 0.05-0.1 parts gum arabic, 0.03-0.1 parts glutamine, 0.01-0.02 parts vitamin B2, 0.01-0.04 parts vitamin B12, and 0.02-0.07 parts glycine. Combined with large particles, it can stimulate saliva secretion, effectively inhibit the formation of dental plaque and tartar, and eliminate bad breath.

This invention relates to the field of pharmaceutical technology, and more particularly to a long-lasting collagen moisturizing and repairing agent, an eye drop, and a method for preparing the same. The long-lasting collagen moisturizing and repairing agent of this invention comprises the following components in parts by weight: 0.01-0.08 parts of type III collagen peptides, 0.05-0.2 parts of sodium hyaluronate, 0.02-0.06 parts of ceramide, 0.01-0.05 parts of lutein, 1-3 parts of propylene glycol, and 0.005-0.02 parts of vitamin B12. The components of this invention are safe and non-irritating. The eye drop prepared according to the above components can maintain a moisturizing effect for 4-6 hours, providing a new approach for the development of eye drops.

The present application provides a kind of fermentation method for improving the content of vitamin B12, by controlling total sugar content to be 1.0-7.0g / 100ml in the fermentation process, under the condition that 5-amino levulinic acid is added in the fermentation process of producing vitamin B12 in the fermentation medium by using Pseudomonas denitrificans, the metabolic pathway of vitamin B12 product synthesis is greatly strengthened.The fermentation method of the present application shortens the fermentation period and effectively improves the fermentation titer in the fermentation process of producing vitamin B12, and has great industrial application prospect.