257 results about "Vitamin B12" patented technology

The invention discloses a cultivation material for increasing the content of B vitamins of straw mushrooms and application of the cultivation material in straw mushroom cultivation. The cultivation material is formed by mixing and fermenting the following components in percentage by weight: 52-72% of pilose antler mushroom dregs, 10-20% of rice straws, 15-25% of waste cotton and 3% of lime. The contents of vitamin B3, vitamin B5, vitamin B6 and vitamin B12 in the volvariella volvacea sporocarp cultivated by adopting the cultivation material disclosed by the invention are obviously increased, the nutritional value of the volvariella volvacea is increased, and the market competitiveness and economic benefits of products are greatly enhanced. The pilose antler mushroom residues are used for replacing part of waste cotton raw materials, the production cost is reduced, meanwhile, efficient recycling of agricultural waste is achieved, and the method is suitable for large-scale industrial production.

The invention relates to the related technical field of daily chemical skin care products, in particular to a water aqua solution for scalp care and a preparation method thereof.The water aqua solution is prepared by mixing multiple raw materials in sequence, and the multiple raw materials comprise, by concentration, 0.005% of mixed cytokines, 0.005% of auxiliary agents and the balance water. The addition amount of the vitamin B12 is 0.00006%; the addition amount of allantoin is 0.2%; the addition amount of nicotinamide is 0.7%; the adding amount of the chamomile extracting solution is 5%; the adding amount of the aloe extracting solution is 5%; the addition amount of the ginkgo biloba extract is 5%; the addition amount of the lucid ganoderma extracting solution is 5%; the addition amount of the polyol preservative is 1%; the total percentage is 100%; according to the application, the mixed cell factors, the vitamin B12, the allantoin, the nicotinamide, the chamomile extracting solution, the aloe, the gingko, the lucid ganoderma, the polyol preservative and the water are mixed, so that the scalp absorption efficiency is improved, the safety of the product can be improved, and the maintenance effect is more obvious; the problems that an existing aqueous solution for skin curing is low in component action efficiency and insufficient in adaptability and safety are solved.

The invention provides a monoclonal antibody 34C1 for resisting vitamin B12. The sequence of a heavy chain CDR1 of the monoclonal antibody 34C1 is as shown in SEQ ID NO: 1, the sequence of CDR2 of the monoclonal antibody 34C1 is as shown in SEQ ID NO: 2, and the sequence of CDR3 of the monoclonal antibody 34C1 is as shown in SEQ ID NO: 3; the sequence of the light chain CDR1 is shown as SEQ ID NO: 4, the sequence of the CDR2 is shown as SEQ ID NO: 5, and the sequence of the CDR3 is shown as SEQ ID NO: 6. The antibody can specifically recognize VB12, and can be developed into an immunodetection reagent based on a competition law principle or a sandwich law principle.

The invention discloses a method for obtaining and culturing ED pathway regulated and controlled engineering bacteria for fermentation production of vitamin B12, a mutant strain is based on ensifer adhesion, an ED pathway key gene edd gene in the ensifer adhesion of a vitamin B12 production strain is overexpressed by a homologous recombination single exchange method, an edd gene overexpressed mutant strain is constructed, and the edd gene overexpressed mutant strain is used for producing the vitamin B12. The purpose of disturbing the ED pathway to realize the metabolic migration of central carbon is achieved, and the method is applied to the production of vitamin B12. A shake flask fermentation experiment proves that overexpression of the edd gene can remarkably promote synthesis of a VB12 product, and the edd gene has good commercial and scientific research values.

The invention discloses modified biogas residue-based hydrothermal carbon, a preparation method thereof and a dry digestion method for kitchen waste, and belongs to the technical field of waste organic matter recycling. The preparation method comprises the following steps: soaking biogas residues in a phosphoric acid solution to obtain pretreated biogas residues; mixing the pretreated biogas residues, transition metal salt, biochar loaded with vitamin B12 and water with the mass being 8-15 times that of the pretreated biogas residues, and performing hydrothermal reaction for 1-4 hours under the conditions that the temperature is 180-250 DEG C and the pressure is 1.5-4 MPa to obtain a hydrothermal product; and activating the hydrothermal product for 1-2 hours under the protective atmosphere of 300-400 DEG C to obtain the modified biogas residue-based hydrothermal carbon. The surface Fe-Cu composite oxide sequentially catalyzes and oxidizes H2S into elemental sulfur and sulfate ions, the vitamin B12-loaded biochar can promote MA directional proliferation, the sulfate ions can competitively inhibit SRB substrate uptake, the quantity ratio of MA to SRB is increased, and the methane production efficiency is effectively improved.

A lamb stomach extract Vitamin B12 preparation composition composed of lamb stomach extract and Vitamin B12 is provided. The lamb stomach extract has a chymosin activity of >40 U / g and a pepsin activity of >15 U / g, and the weight ratio of the lamb stomach extract and Vitamin B12 is 100000:0.5-5, which is useful in the treatment of moderate-to-severe chronic atrophic gastritis, intestinal metaplasia reversal, and gastric cancer prevention. Lamb stomach extract and Vitamin B12 produced synergistic or synergistic effects in specific ratios.

The invention belongs to the technical field of biological detection, and particularly relates to a method for synchronously and quantitatively detecting various folic acid metabolism related substances in trace plasma or serum, which comprises the following steps of: detecting a pretreated trace plasma or serum sample by adopting a liquid chromatography-tandem mass spectrometry method; the following nine folic acid metabolism related substances are synchronously and quantitatively detected: 5-formyl tetrahydrofolic acid, folic acid, 5-methyl tetrahydrofolic acid, S-adenosine-L-methionine, S-adenosine-L-homocysteine, vitamin B12, pyridoxamine, pyridoxine and pyridoxal. The invention further relates to application of the detection method in related detection of child neurodevelopment. According to the detection method, only trace plasma or serum is used, methotrexate is used for replacing an expensive isotope internal standard for more economical detection, and nine folate metabolism related substances can be accurately quantified at the same time. Therefore, the detection method disclosed by the invention has important clinical significance and wide market application prospect.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

A composition, preferably for caring for and / or making up keratin materials, comprising: a) polydatin and / or derivatives thereof, b) at least 0.0001 wt. % of at least one Vitamin B12, relative to the total weight of the composition, and c) at least one hydrotrope.

Various benefits to intestinal health are made by delivery of an active agent to the colon of an animal, including a human. Active ingredients include of riboflavin, vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, folic acid, β-carotene, vitamin B1, niacin, vitamin B5, vitamin B6, biotin, vitamin B12, omega-3 fatty acids and combinations thereof. Benefits include increased concentration of at least one short-chain fatty acid its salt thereof in the intestine, increased microbiome diversity in the intestine, increased beneficial bacteria in the intestine, increased the butyrate synthesis pathway in the intestine, improved barrier function of the intestine, reduced redox potential of the gut, reduced amount of gas produced in the intestine; and stimulation of intestinal immune responses.

The invention relates to the technical field of biology, in particular to a method for producing vitamin B12 through fermentation of propionibacterium shermangii, which comprises the following steps: inoculating propionibacterium shermangii into a seed culture medium to culture a seed solution; inoculating the seed solution into a fermentation culture medium for fermentation; after fermentation is finished, thalli are removed in a centrifugal separation or filtration mode, a fermentation solution is subjected to ultrafiltration concentration, a concentrated solution is purified through ion exchange chromatography or affinity chromatography, and a vitamin B12 crude product is obtained; and further purifying by adopting a crystallization method to obtain a high-purity vitamin B12 finished product. According to the method for producing the vitamin B12 through fermentation of the propionibacterium shermannii, the yield and the purity of the vitamin B12 are remarkably improved and the production cost is reduced by accurately controlling fermentation conditions, optimizing culture medium components, introducing an intelligent regulation and control system and adopting a specific purification process, so that the method is suitable for industrial production. The problems of low yield, unstable purity, difficult production process control and the like of the vitamin B12 in the prior art are solved.

The invention provides an anti-idiotypic nano antibody 28E1 of vitamin B12. A variable region sequence of the anti-idiotypic nano antibody 28E1 is shown as SEQ ID NO: 1. The antibody sequence of the anti-idiotype nano antibody 28E1 of the vitamin B12 is as shown in SEQ ID NO: 2. The antibody can specifically recognize the anti-VB12 monoclonal antibody 34C1 and inhibit the combination of the anti-VB12 monoclonal antibody 34C1 and VB12, and can be used for developing an immunodetection reagent based on a competition law principle.

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

The invention provides vitamin B12 modified microspheres capable of efficiently loading His tag recombinant protein as well as a preparation method and application of the vitamin B12 modified microspheres, and belongs to the technical field of biological medicines. The preparation method of the vitamin B12 modified microsphere for efficiently loading the His tag recombinant protein comprises the following steps: (1) carrying out esterification reaction on unsaturated fatty acid containing sulfydryl, disulfide bond or olefinic bond, saturated fatty acid and vitamin B12 to prepare an amphiphilic vitamin B12-lipid conjugate; and (2) taking the amphiphilic vitamin B12-lipid conjugate, PLGA (poly (lactic-co-glycolic acid)), a multi-sulfydryl cross-linking agent and a photoinitiator as raw materials, and preparing the raw materials into microspheres by adopting a microfluidic method, so as to obtain the vitamin B12 modified microspheres capable of efficiently loading various His tag recombinant proteins. The microsphere provided by the invention overcomes the problems of protein load stability and burst release of traditional microspheres, and various different His tag recombinant growth factors can be flexibly selected according to the requirements on different growth factors in treatment of complex bone defects caused by different pathogenesis.

The invention belongs to the technical field of biological medicines, and particularly relates to a nutritional composition as well as a preparation method, application and a product thereof. The nutritional composition provided by the invention consists of nicotinamide, B vitamins and a fungal polysaccharide compound, the vitamin B consists of vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid; the fungal polysaccharide compound is composed of a lucid ganoderma extract, a mushroom extract, an agaricus blazei murill extract, a hericium erinaceus extract, a coprinus comatus extract, a grifola frondosa extract and a cordyceps militaris extract. Western medicine source substances (nicotinamide and B vitamins) and traditional Chinese medicine source substances (fungal polysaccharide compounds) are combined, and the nutrition composition is prepared through combination of the Chinese medicine and the western medicine. The nutritional composition has remarkable effects of enhancing immunity, delaying senescence, resisting fatigue and resisting oxidation, and shows extremely wide application prospects in the field of preparation of health-care products, medicines and cosmetics.

The invention provides a novel filtering membrane used in a VB12 production process and a preparation method thereof, and belongs to the technical field of vitamin B12 production.The preparation method comprises the following steps that firstly, graphene oxide is obtained after graphene powder is oxidized, a diphenyl substitute is grafted to the surface of the graphene oxide, and an intermediate is obtained; 2, carrying out mixed reaction on the modified chitosan and the intermediate to obtain modified graphene; and 3, mixing the modified graphene with the cellulose ester membrane casting solution, and preparing the novel filtering membrane used in the VB12 production process through a phase inversion method. Through collaborative design of a graphene-chitosan hybrid structure and a polyphenylsilane cross-linked network, high selectivity, strong pollution resistance and chemical corrosion resistance of the filtering membrane are realized, and the filtering membrane is worthy of popularization.

The present invention relates to a fluorescent detection kit for detecting hydroxocobalamin receptor antibodies in central nervous system vitamin B12 deficiency and its application, which can efficiently and accurately detect the presence and concentration of anti-CD320 antibodies in patient serum or cerebrospinal fluid. The kit uses a specific antibody to bind to the CD320 protein produced by cells expressing the CD320 gene, which then binds to a fluorescently labeled secondary antibody to generate a fluorescent signal, thereby diagnosing central nervous system vitamin B12 deficiency. The base sequence in this application ensures the effective expression of the CD320 gene, which is crucial for improving the sensitivity and specificity of the test.

The present invention provides a homocysteine-reducing composition that rapidly lowers blood homocysteine ​​levels to an appropriate level without causing any concerns about side effects. [Solution] A composition for reducing homocysteine ​​levels, comprising one or more B vitamins selected from vitamin B6, vitamin B12, and folic acid, and β-NMN as active ingredients. The β-NMN content is in the range of 22.32% to 89.29% by weight. Vitamin C and vitamin B2 may also be added.

The present invention discloses a method containing VB 12 The production method of the medicinal plant additive for suckling pig feed and the product thereof relate to the field of suckling pig breeding. The production method of the medicinal plant additive for suckling pig feed of the present invention comprises the following steps: (1) crushing cardamom, tricholoma, oregano and inulin respectively and enzymolyzing them with cellulase, and then mixing them in proportion, or first mixing cardamom, tricholoma, oregano and inulin in proportion, and then crushing and enzymolyzing them with cellulase to obtain a primary enzymolysis material; (2) adding potassium dihydrogen phosphate and oxalic acid to the primary enzymolysis material, and continuing to degrade to obtain a hot-cooked enzymolysis material; (3) mixing the hot-cooked enzymolysis material with molasses, wormwood, glucose, and yeast extract to obtain a mixture, adding sterilized tap water and Clostridium butyricum liquid, and fermenting to obtain a primary fermentation material; (4) adding pretreated Sanguisorba officinalis and molasses to the primary fermentation material, and continuing to ferment. The medicinal plant additive for suckling pig feed of the present invention can significantly reduce the feed-to-meat ratio of suckling pigs and significantly increase daily weight gain.

The present invention provides a vitamin premix and a preparation process thereof, and belongs to the technical field of food additives, the preparation process is as follows: fat-soluble vitamin mixed particles, mixed water-soluble vitamins and mixed minerals are added into a mixer for mixing, then vitamin C, magnesium taurate, amino acids and tricalcium phosphate are added for continuous mixing, and sieving is performed to obtain the vitamin premix. Wherein the fat-soluble vitamin mixed particles comprise the following raw materials: vitamin A, vitamin E acetate, vitamin D3, vitamin K2, Arabic gum, modified protein and beta-cyclodextrin; the mixed water-soluble vitamins comprise the following raw materials: vitamin B1, vitamin B2, vitamin B5, vitamin B12, folic acid and nicotinamide; the mixed mineral substance comprises the following raw materials: ferrous glycinate, calcium citrate, magnesium citrate and zinc glycinate. The vitamin premix prepared by the invention can be used as a nutritional supplement in various foods, is convenient to use, and has good stability and mixing uniformity.

Mood support compositions are described herein. For example, this document provides mood support compositions containing saffron, ashwagandha, and vitamin B12. Such compositions may be used to, e.g., support a positive mental outlook, promote emotional health, support general well-being, and reduce feelings of occasional stress.

The invention relates to the technical field of biological fermentation, in particular to a method for improving the fermentation stability of vitamin B12, which comprises the following steps: acquiring a microbial strain of vitamin B12 and activating; preparing a culture medium suitable for fermentation of vitamin B12 according to a composite carbon source system, an optimized nitrogen source combination, key elements, other nutritional ingredients, plant three elements and a modified buffer agent; according to the fermentation stages, the fermentation conditions are accurately controlled stage by stage; an online monitoring system is established, key parameters in the fermentation process are monitored in real time, a neural network model is established, and the stability and high efficiency of the fermentation process are ensured. According to the method for improving the fermentation stability of the vitamin B12, a diversified carbohydrate mixture is used as a carbon source, a nitrogen source combination is optimized, key elements are accurately added, a specific buffer system is constructed, and an online monitoring system and a neural network model are established for big data analysis and intelligent prediction; the synthesis of vitamin B12 is promoted, and the stability and high efficiency of the fermentation process are ensured.

The invention discloses NaYF4: Yb, Er (at) NaYF4 (at) SiO2 (at) AgNPs as well as a preparation method and application thereof in vitamin B12 detection. According to the method, a core NaYF4: Yb, Er and a core shell NaYF4: Yb, Er (at) NaYF4 are prepared through a high-temperature solvothermal reaction; and carrying out hydrophilic modification and fluorescence enhancement modification to obtain the nano probe. Compared with the prior art, the invention has the following advantages: (1) the NaYF4: Yb, Er (at) NaYF4 (at) SiO2 (at) Ag NPs can absorb exciting light at 980nm and convert the exciting light into visible fluorescence, and through an energy transfer mode, an up-conversion fluorescence emission peak and an ultraviolet absorption peak of vitamin B12 coincide, so that rapid detection is realized; (2) the nanoparticles and the application method have the advantages of high sensitivity, convenience and rapidness in operation, good specificity and the like.

The invention relates to the technical field of biology, in particular to a method for increasing the yield of vitamin B12 through an ultrasonic-assisted fermentation method, which comprises the following steps: screening a propionibacterium strain and inoculating the propionibacterium strain into a seed culture medium to obtain a seed solution; a carbon source of the fermentation culture medium is supplied in stages, glucose is used in the early stage, modified glycerol is used in the later stage, the proportion of a nitrogen source is dynamically adjusted, and metal ions and vitamins are added; performing ultrasonic treatment on the fermentation liquor in the early stage of the logarithmic growth phase in the fermentation process; extracting vitamin B12 by adopting an ultrasonic coupling deep-eutectic solvent method; and purifying the supernate to obtain a vitamin B12 product. According to the method for improving the yield of the vitamin B12 through the ultrasonic-assisted fermentation method, the production process of the vitamin B12 is optimized and the yield and the purity of the vitamin B12 are improved by supplying a carbon source in stages, dynamically adjusting the proportion of a nitrogen source, carrying out ultrasonic treatment in the early stage of a logarithmic phase and adopting an ultrasonic coupling deep-eutectic solvent extraction technology.

The invention discloses a cell protection solution for venous transfusion as well as a preparation method and application of the cell protection solution. The cell protection liquid comprises 4 to 8 mg / ml of sodium chloride, 0.1 to 0.5 mg / ml of potassium chloride, 2.8 to 3.5 mg / ml of sodium lactate, 0.1 to 0.3 mg / ml of calcium chloride, 0.15 to 0.3 [mu] g / ml of glucose, 0.01 to 0.03 ml / ml of 20% human serum albumin, 4 to 8 [mu] g / ml of vitamin E, 0.2 to 2 [mu] g / ml of vitamin B12, 0.5 to 2 [mu] g / ml of folic acid, 4 to 8 [mu] g / ml of L-aspartic acid and 0.5 to 2 [mu] g / ml of glutathione. The cell preserving fluid provided by the invention can effectively protect cells for a long time, can be directly transfused back through veins, does not need special treatment, is convenient for clinical application, and has better application prospect and development potential in the development of the clinical treatment of the cells towards the drug direction.

This invention provides a method for producing vitamin B12 using microchannel reaction technology. The method involves acidifying, hydrolyzing, filtering, and analyzing the vitamin B12 fermentation broth, followed by a cyanidation reaction in a microchannel reactor. Further filtration, analysis, and concentration yield vitamin B12. This method produces cyanide-treated vitamin B12 with a thorough conversion reaction and increased yield. It also reduces the amount of cyanide used, thus reducing pollution and costs. The equipment has good airtightness, and the small amount of hydrogen cyanide gas generated is completely recovered and centrally treated, greatly reducing harm to the environment and human health. This method is environmentally friendly and contributes to green production.

The invention relates to a large-particle dog and cat oral health-care functional food wrapped with instant freeze-dried particles and a preparation method thereof, and belongs to the technical field of pet food. According to the scheme, an external spraying system comprises grain base material particles, 0.1 to 0.15 part of lecithin, 0.002 to 0.003 part of monostearin, 0.001 to 0.003 part of vitamin E and 0.8 to 1.2 parts of chicken oil, and an instant freeze-dried particle layer comprises 0.1 to 0.2 part of fucoidan, 0.02 to 0.05 part of lysozyme, 0.04 to 0.08 part of tea polyphenol, 0.02 to 0.04 part of zinc gluconate, 0.05 to 0.1 part of Arabic gum, 0.03 to 0.1 part of glutamine, 0.01 to 0.02 part of vitamin B2, 0.01 to 0.04 part of vitamin B12 and 0.02 to 0.07 part of glycine. The toothpaste can effectively inhibit the formation of dental plaque and dental calculus and remove halitosis.

The invention provides a refreshing and anti-fatigue composition as well as a preparation method and application thereof, and relates to the technical field of nutritional supplements. The refreshing and anti-fatigue composition is prepared from the following components in parts by mass: 1000 to 5000 parts of phosphatidylserine, 300 to 500 parts of taurine, 10 to 20 parts of nicotinamide, 2 to 3 parts of vitamin B1, 1 to 1.5 parts of vitamin B6 and 0.001 to 0.002 part of vitamin B12. The refreshing and anti-fatigue composition alleviates the problems of complex material consumption, large component intake, too high sugar content and the like of the refreshing and anti-fatigue composition in the prior art. The refreshing and anti-fatigue composition can promote energy metabolism, improve brain functions and relieve physical and mental fatigue. The formula supports nervous system health through multiple ways, helps people to keep sober and concentrate, and reduces the feeling of fatigue at the same time.

The invention belongs to the technical field of vitamin content detection, and particularly relates to a method for measuring the content of various water-soluble vitamins B. The water-soluble vitamins B comprise vitamin B1, vitamin B2, vitamin B3, vitamin B6, vitamin B7, vitamin B12 and nicotinamide. A high performance liquid chromatograph and an electrospray detector are used as detection instruments, and chromatographic conditions are as follows: a mobile phase A is ammonium formate or ammonium acetate, a mobile phase B is methanol, and gradient elution is performed; the gradient elution procedure is as follows: a solvent of a test sample is a mixed solution of acetonitrile, water and glacial acetic acid, and the volume ratio of the acetonitrile to the water to the glacial acetic acid is 5: 95: 0.5; the atomization temperature of the electrospray detector is 35 DEG C; the method is better in separation degree and higher in specificity and sensitivity.