36 results about "Pyridoxal" patented technology

The invention discloses a method for efficiently producing pyridoxal phosphate based on a three-enzyme cascade reaction and a recombinant strain, and belongs to the technical field of biological engineering. The recombinant escherichia coli for co-expressing the pyridoxine phosphate phosphatase mutant, the pyridoxine phosphate oxidase and the hydrogen peroxide decomposing enzyme is constructed, three-enzyme synergy is realized by optimizing the copy number of a carrier, and the catalytic efficiency of a recombinant strain as a cell catalyst is improved. The invention further provides a one-pot method for preparing pyridoxal phosphate, the production cost is reduced, an efficient, green and economical biosynthesis path is provided for industrial production of PLP, and the method can be widely applied to the fields of food, medicine and the like and has important industrial value and application prospects.

The invention relates to the technical field of genetic engineering, in particular to a recombinant microorganism and application thereof in preparation of glycine. The application comprises the following steps: producing glycine by adopting the recombinant microorganism; the recombinant microorganism comprises an enzyme combination and / or a nucleic acid molecule combination. The enzyme combination comprises threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase; the threonine aldolase comprises an amino acid sequence as shown in SEQ ID NO. 1. The nucleic acid molecule combination is used for encoding the enzyme combination. According to the invention, threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase are converted into microorganisms, and the constructed recombinant microorganisms can be used for decomposing threonine to produce glycine; and a specific system formed by specific isopropanol dehydrogenase, ethanol dehydrogenase and isopropanol dehydrogenase can also have a relatively high conversion rate under the condition of no addition of pyridoxal phosphate (PLP), and has important significance and economic value.

The invention relates to a pyridoxal phosphate (PLP) synthesis method based on cascade biological catalysis and capable of realizing ATP autonomous regeneration. The method comprises the following steps: constructing engineering bacteria for co-expressing pyridoxal phosphate kinase (PdxK) and polyphosphate kinase (PPK) to prepare a whole-cell catalyst or a crude enzyme solution; under the condition that ATP does not need to be exogenously added, pyridoxal, ADP and polyphosphate serve as substrates, cascade reaction is carried out under the action of a catalyst, ATP is regenerated in situ, and PLP synthesis is driven. A wild enzyme system is adopted, and the yield of PLP exceeds 95% within 4 hours of reaction; after PdxK mutation optimization, the reaction time is shortened to about 2 hours, and conversion can be completed. According to the invention, a polyphosphate-driven ATP regeneration system is coupled with PLP biosynthesis, so that the cofactor cost is remarkably reduced, a feasible technical path is provided for industrial biological manufacturing of PLP, and the application of polyphosphate in cyclic regeneration of cofactors is expanded.

This invention relates to a pyridoxal phosphate-responsive biosensor and its application, belonging to the field of synthetic biology biosensor technology. This invention is the first to discover and identify the relationship between pyridoxal phosphate and Cgl1185-P. cgl2838 The transcriptional regulation and response relationship between these factors was investigated, and this relationship was used as the basis for constructing a biosensor responsive to pyridoxal. The transcription factor regulatory system Cgl1185-P derived from Corynebacterium glutamicum was used. cgl2838 A biosensor for the specific detection of pyridoxal phosphate was constructed by assembling a reporter gene as the main component onto a plasmid, enabling effective detection of pyridoxal phosphate concentrations in intracellular or culture environments. This biosensor possesses advantages such as clear structure, high sensitivity, and good host adaptability, and can be used for fermentation process monitoring, cofactor metabolism research, and screening of relevant strains, showing promising application prospects in the fields of cofactor concentration monitoring and metabolic regulation.

The invention relates to the field of food biotechnology and fermentation engineering, and discloses lipid-lowering health-preserving vinegar capable of strengthening an amino acid metabolic pathway and a preparation process thereof, and the lipid-lowering health-preserving vinegar capable of strengthening the amino acid metabolic pathway is prepared by fermenting soybean protein isolate, silkworm chrysalis protein powder, broken rice, oat bran, a metabolism anchoring regulation composite emulsion and a plant extract. According to the metabolism anchoring regulation composite emulsion, carrier oligopeptide, pyridoxal-5-phosphoric acid and metal ions are utilized to construct a coordination system, and the microbial amino acid metabolism flow direction is directionally regulated. The preparation process is completed by adopting lactobacillus plantarum and acetobacter pasteurianus through anaerobic-aerobic variable-temperature variable-dissolved-oxygen coupling fermentation. Through a metabolic network intervention strategy, the enrichment efficiency of gamma-aminobutyric acid and hydrophobic active oligopeptide in the finished product is remarkably improved, and the flavor of the product is improved, so that the table vinegar is endowed with stronger auxiliary lipid-lowering and pressure-lowering effects.

The invention provides a composition comprising a Plasmodium species multimeric protein capable of forming a nanoparticle and at least one antigen of interest, wherein the multimeric protein comprises a monomer chosen from Plasmodium species pyridoxal 5'-phosphate synthase (PLP), chaperone 60 protein (Cpn60), and caseinolytic protease (Clp). In embodiments, the invention provides the composition wherein the monomers assemble to form a multimeric nanoparticle and / or which comprises an adjuvant. The invention further provides a nucleic acid encoding the multimeric protein and the at least one antigen of interest, E. coli comprising the nucleic acid, and the use of such in a method for producing the inventive multimeric protein and the at least one antigen of interest. Also provided are a method and use of the inventive multimeric protein and the at least one antigen of interest for immunizing a subject against Plasmodium species.

The invention relates to a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, and belongs to the technical field of biosynthesis. In order to solve the existing problem of serious environmental pollution, the invention provides a biosynthesis method of pyridoxal phosphate or pyridoxine phosphate, which comprises the following steps: in the presence of polyphosphate, magnesium salt, ATP (adenosine triphosphate), pyridoxal kinase and polyphosphate kinase, performing phosphorylation reaction on pyridoxal or pyridoxine and acid salts thereof, thereby obtaining the pyridoxal phosphate or pyridoxine phosphate. The temperature of the phosphorylation reaction is controlled to be 30-38 DEG C, after the reaction is finished, protein separation is conducted on reaction liquid, mother liquor is separated, the compound pyridoxal phosphate or pyridoxine phosphate shown in the formula I is obtained, and the mother liquor is recycled and reused. According to the method, a large amount of ammonium acetophosphate does not need to be additionally added, operation is simpler, mother liquor can be recycled, a large amount of waste water is prevented from being discharged, environment friendliness is better achieved, and the yield is high.

This invention relates to the field of biomaterial preparation technology, and in particular to a morphology-controllable mesoporous hydroxyapatite nanosphere, its preparation method, and its applications. The preparation method includes: S1, mixing an aqueous solution of pyridoxal phosphate with an aqueous solution of calcium chloride, adjusting the pH of the mixed solution to 7-11 to obtain a precursor solution; S2, placing the precursor solution in a microwave reactor for reaction to obtain mesoporous hydroxyapatite nanospheres. The preparation method provided by this invention is simple, the reaction conditions are easily controlled, and it is highly safe and reliable. It enables precise control of the morphology and particle size of the hydroxyapatite microspheres, and the prepared product exhibits excellent biocompatibility and structural regularity. It has good industrial application prospects in the field of biomedical implants such as bone tissue repair and is suitable for large-scale production.

The invention provides an omega-transaminase mutant and application thereof, belongs to the technical field of microbial pharmacy and pharmaceutical chemical engineering, and particularly provides the omega-transaminase mutant, and the amino acid sequence of the omega-transaminase mutant is shown as SEQ ID NO.4 or SEQ ID NO.6; the omega-transaminase mutant provided by the invention not only has high enzyme activity, but also can be used for preparing 2-thiophenemethylamine by using 2-thiophenecarboxaldehyde; the engineering escherichia coli for expressing the omega-transaminase mutant is used for performing whole-cell catalysis to prepare the 2-thiophenemethylamine, so that the conversion rate is high, and the concentration of the coenzyme pyridoxal phosphate used in the reaction is reduced.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a preparation method of a directional pore channel integral material and a method for constructing an enrichment device and establishing a rapid phosphopeptide enrichment method by using the directional pore channel integral material. The preparation method comprises the following steps: preparing a CS-PC integral material with directional pore channels from chitosan, zirconium amino phthalocyanine and polyethylene glycol diglycidyl ether through a directional freezing method and a chemical crosslinking method, and then modifying the CS-PC integral material with 5 '-phosphopyridoxal and zirconium oxychloride to obtain the CS-PC PLP integral material. The CS-PC-PLP integral material prepared by the invention has anisotropy and a directional pore channel structure, is convenient to fix in a pipette tip and is combined with a row pipettor for rapid enrichment; the CS-PC coated PLP integral material has the advantages of green and cheap raw materials and strong reusability.

The invention relates to the technical field of polymer synthesis, in particular to polymer microspheres and a preparation method and application thereof.The preparation method of the polymer microspheres comprises the following steps that pyridoxal, an anhydrous aprotic polar solvent, organic alkali and methacryloyl chloride are mixed to be subjected to an esterification reaction under the conditions of protective gas and light shielding, and the polymer microspheres are obtained. Pyridoxal methacrylate is prepared; dissolving inorganic salt and a dispersing agent in water to prepare a water phase; the preparation method comprises the following steps: mixing pyridoxal methacrylate, styrene, divinyl benzene and an initiator to prepare an oil phase; heating the water phase to 65-75 DEG C, and adding the oil phase into the water phase under a stirring condition to prepare a suspension dispersion system; under the protection of protective gas, the suspension dispersion system is subjected to a reaction for 8-12 h at the temperature of 65-75 DEG C, the polymer microspheres are prepared, and the polymer microspheres can keep efficient adsorption of uranyl ions within a wide pH range.

The invention discloses a chain-shaped silver complex as well as a preparation method and application thereof. The invention relates to a synthesis method of a chain-like silver complex, which comprises the following steps: carrying out condensation reaction on pyridoxal and p-methylbenzenesulfonyl hydrazide to generate a pyridoxal condensed p-methylbenzenesulfonyl hydrazide ligand, reacting the ligand with silver nitrate, slowly volatilizing the reaction liquid for 3-5 days, and separating out a yellow crystal of the chain-like silver complex. The crystal is subjected to structural analysis by adopting an X-ray single crystal diffraction technology, and the result shows that the crystal is a novel chain-shaped coordination polymer. The chain-shaped silver complex shows remarkable antibacterial activity on staphylococcus aureus, pseudomonas aeruginosa, methicillin-resistant staphylococcus aureus and multi-drug-resistant pseudomonas aeruginosa, and the in-vitro antibacterial effect of the chain-shaped silver complex is superior to that of silver nitrate, a ligand and a methicillin monomer; the application potential of the compound serving as an efficient antibacterial agent in the aspect of replacing or supplementing existing anti-infection drugs is shown. The structure of a pharmaceutically acceptable compound of the chain-shaped silver complex is shown as a formula (I) in the specification.

The invention discloses a D-amino acid transaminase mutant and a method for preparing 4-chloro-D-phenylalanine by using the D-amino acid transaminase mutant. The method comprises the following steps: in the presence of a buffer solution, an amine source and pyridoxal phosphate, catalyzing a compound II by using the D-amino acid transaminase mutant to generate a transaminase reaction to generate 4-chloro-D-phenylalanine, the amino acid sequence of the D-amino acid transaminase is as shown in SEQ ID NO: 1; the structural formula of the 4-chloro-D-phenylalanine is shown as a compound I. According to the mutant and the method, efficient and high-stereoselectivity biological catalytic synthesis of the 4-chloro-D-phenylalanine is realized on the basis of specific catalysis of the D-amino acid transaminase mutant obtained by directed evolution on 4-chlorphenyl pyruvic acid.

A pyridoxal kinase mutant is provided. To solve the problem of low concentration of existing enzyme catalyzed products, provided is a pyridoxal kinase mutant having an amino acid sequence selected from an amino acid sequence as shown in SEQ ID NO: 1 in which lysine at position 229 is mutated to alanine, phenylalanine, arginine, threonine, histidine, serine, tyrosine, valine, leucine, isoleucine, proline, aspartic acid, or glutamic acid; and the pyridoxal kinase mutant is used to catalyze the synthesis of pyridoxal phosphate from pyridoxal.

The invention relates to the technical field of microorganisms, and discloses an improved LST broth culture medium and application, and the improved LST broth culture medium comprises tryptone, ammonium chloride, lactose, dipotassium phosphate, monopotassium phosphate, sodium lauryl sulfate, Rpf recombinant protein, magnesium sulfate, sodium pyruvate and pyridoxal phosphate. The improved LST broth culture medium disclosed by the invention can be used for successfully enriching the VBNC-state escherichia coli, so that researchers can successfully detect the VBNC-state escherichia coli in a culture manner.

The disclosed invention relates to a chemical composition that can be taken orally and supplements an unhealthy diet and lifestyle. In one aspect of the invention the chemical composition includes Retinol Palmitate, Thiamine, Riboflavin, Niacin, Nicotinamide mononucleotide (NMN), Pyridoxal, Folic Acid, Cyanocobalamin, Cholecalciferol, Zinc, Manganese, Copper, Calcium, Magnesium, Iron, L-Selenomethionine, Lysine, Phenylalanine, L-Threonine, L-Methionine, L-Histidine, L-Tryptophan, L-Leucine, L-Isoleucine, L-Valine, Cinnamon Bark Powder, and Curcumin.

The application discloses a SNP marker related to wheat kernel pyridoxal content and application thereof. The application provides application of a substance for detecting a SNP site Ta-3B-SNP haplotype in a wheat genome in any one of the following: A1) identifying or assisting in identifying kernel pyridoxal content; A2) screening or assisting in screening wheat with high kernel pyridoxal; A3) breeding wheat with high kernel pyridoxal; A4) wheat genetic breeding; the SNP site Ta-3B-SNP is the 74th site of SEQ ID NO:1. The application finds a wheat kernel pyridoxal (PL) content related site, and develops a molecular marker according to the SNP site, the marker is a KASP marker, has good repeatability, low cost, is beneficial to screening wheat strains or varieties with high pyridoxal (PL) content, and has important significance for cultivating new wheat varieties with high pyridoxal (PL) content.

The invention discloses a method for producing gamma-aminobutyric acid through double-bacterium synergistic fermentation of lignocellulose hydrolysate, and belongs to the technical field of microbial engineering. The method comprises the following steps: constructing a recombinant pediococcus acidilactici (Pediococcus acidilactici FR-XY) engineering bacterium, and constructing a recombinant pediococcus acidilactici (Pediococcus acidilactici) FR-XY; the method comprises the following steps: carrying out staged fermentation culture on lactobacillus hilgardii FR-1012 and recombinant pediococcus acidilactici FR-XY by utilizing lignocellulose hydrolysate, so as to obtain mixed fermentation liquor; centrifuging the mixed fermentation liquor, collecting mixed thalli, resuspending the mixed thalli in a conversion solution containing L-glutamic acid and coenzyme 5-phosphopyridoxal, and carrying out whole-cell conversion reaction to obtain a conversion solution containing gamma-aminobutyric acid. According to the method, the lignocellulose hydrolysate can be utilized for double-bacterium synergistic fermentation, and full utilization of a multi-element carbon source in the lignocellulose hydrolysate and efficient synthesis of gamma-aminobutyric acid are achieved.

Provided herein are prodrugs of phenylalanamines (e.g., magical phenylethylamines) incorporating a vitamin B6-based prodrug moiety (e.g., pyridoxal). Also provided are methods of making such compounds, pharmaceutical compositions thereof, and methods of use thereof, such as for the treatment of psychological health disorders, neurodegenerative disorders, pain disorders, and inflammation, including as part of adjuvant therapy as magical agents. (I) (I)

The present application relates to the field of animal reproduction technology, and in particular to a sperm protection agent for improving sperm preservation quality and embryo development potential and application thereof, wherein the effective components of the sperm protection agent are selected from any one or several of acetyl carnitine, glycerophosphocholine, tauroursodeoxycholic acid, glutamic acid, sunflower acylcarnitine, pyridoxal phosphate and glyco ursodeoxycholic acid. Based on the regulation of the OR4C13-G13 / Golf signal axis related to sperm capacitation of the effective components, sperm viability can be maintained during preservation, premature capacitation can be inhibited, and the fertilization rate and embryo development potential can be improved. The present application is suitable for the normal temperature preservation of pig semen and can be extended to other mammalian or human assisted reproduction fields.

This invention belongs to the field of enzyme engineering technology, specifically relating to an EcCAT mutant of catalase and its applications. The mutant is obtained by mutating at least one amino acid from the following positions in SEQ ID NO:1: Ile (126th position), Ser (167th position), Val (199th position), Gly (200th position), Asn (201st position), Phe (206th position), Ala (211th position), Phe (214th position), Gly (273rd position), Ala (389th position), Phe (391st position), Leu (407th position), and Gly (410th position). Compared to wild-type catalase, the EcCAT mutant of this invention exhibits higher catalytic efficiency. When used in combination with pyridoxine 5'-phosphate oxidase, the whole-cell catalytic synthesis of pyridoxal 5'-phosphate can be completed in just 5 hours, with a conversion rate exceeding 99%.

This invention provides an ω-transaminase mutant and its applications, belonging to the fields of microbial pharmaceutical and pharmaceutical chemical technology. Specifically, it provides an ω-transaminase mutant with an amino acid sequence as shown in SEQ ID NO.4 or SEQ ID NO.6. The ω-transaminase mutant provided by this invention not only has high enzyme activity but also can prepare 2-thiophene methylamine using 2-thiophene carboxaldehyde. The whole-cell catalytic preparation of 2-thiophene methylamine using engineered Escherichia coli expressing the ω-transaminase mutant not only has a high conversion rate but also reduces the concentration of the coenzyme pyridoxal phosphate used in the reaction.

This utility model discloses an adsorption and impurity removal device for the production of pyridoxal phosphate, comprising a base plate with legs at each of the four corners of the lower end face of the base plate, and tanks on both the left and right sides of the upper end face of the base plate. Both tanks have detachable covers on their upper ends, and both tanks have detachable filters inside. Both covers have stirring mechanisms. A feeding pipe extends through the upper left end face of the left tank, and a discharge pipe extends through the lower right end face of the right tank. A pump body is located at the center of the upper end face of the base plate, with a suction pipe connected to its left end face. The suction pipe extends through the upper right end face of the left tank, and a feeding pipe is connected to the upper end face of the pump body. The feeding pipe extends through the lower left end face of the right tank. Both tanks have drain pipes extending through their bottom ends. Through the tank design, multi-stage adsorption and impurity removal is achieved, effectively improving the purity and production efficiency of pyridoxal phosphate.

The invention discloses a preparation method of sitagliptin base, and relates to the technical field of compound preparation, and the preparation method comprises the following steps: S1, adding 75 parts of water and 16 parts of hydrochloric acid into a three-neck bottle, and cooling to 0 DEG C; s2, the pH is adjusted to 7.5, 42.5 parts of a triethanolamine solution, 0.2 part of pyridoxal phosphate and 57 parts of DMSO are added, and the temperature is controlled to be lower than 10 DEG C; s3, adjusting the pH value to 8.5, adding 40 parts of liquid transaminase, and slowly heating to 40-45 DEG C; s4, when the temperature rises to 45 DEG C, 20 parts of diketone is dropwise added, the temperature is controlled to be 45 DEG C, the pH is adjusted to 8.5, and heat preservation is conducted for 24 h; s5, performing suction filtration and extraction, cooling and crystallizing through methyl tert-butyl ether to obtain a white solid, and drying to obtain a finished product sitagliptin base; preparation equipment adopted in the preparation method comprises a closed cylinder, a liquid separation mechanism, an organic phase receiving box, a water phase receiving box and the like, closed control in the liquid separation extraction process is achieved, recycling of the extraction agent is achieved, and the volatilization and dissipation amount of the extraction agent in the preparation process is effectively reduced.

The invention provides a kit for detecting homocysteine and metabolites thereof in serum. The kit comprises a calibrator and a sample releasing agent, the calibrator comprises a component 1 and a component 2; the component 1 comprises a BSA (Bovine Serum Albumin) matrix and a first protective agent; the component 2 comprises homocysteine, metabolite of the homocysteine and a second protective agent; each of the first protective agent and the second protective agent is prepared from ascorbic acid, dithiothreitol, N-acetyl-DL-penicillamine and EDTA (Ethylene Diamine Tetraacetic Acid). According to the present invention, the 5-methyltetrahydrofolic acid and the homocysteine which are easily oxidized are well protected, and the synchronous detection of the folic acid, the 5-methyltetrahydrofolic acid, the vitamin B2, the pyridoxal phosphate, the methylmalonic acid, the homocysteine and the methionine is achieved. The kit has good stability at 4 DEG C and can meet the use requirement.

This utility model discloses a reaction apparatus for the production of pyridoxal phosphate, including a shell, a reaction tank rotatably disposed inside the shell, bearings disposed on the upper and lower sides of the reaction tank and at the connection points with the shell, a stirring shaft rotatably disposed inside the reaction tank, stirring rods disposed on the outer wall of the stirring shaft, multiple stirring rods disposed and evenly distributed, and baffles disposed on the inner wall of the reaction tank, multiple baffles disposed and staggered with the stirring rods, and a motor disposed at the upper end of the shell, so that the user can control the motor to drive the reaction tank to rotate by gears, and the motor will also drive the stirring shaft and stirring rods to rotate through pulleys and belts. Since the rotation direction of the reaction tank and the stirring shaft is opposite, the rotation direction of the baffles and the stirring rods is opposite, so that the solution inside the reaction tank is fully stirred, thereby enabling it to react quickly and helping to increase the production efficiency of pyridoxal phosphate.

The application discloses a composition with synergistic hypoglycemic effect and a preparation method and application thereof, and belongs to the field of biomedical technology. The composition is prepared from defatted soybean meal powder, pueraria powder, polygonatum sibiricum redoute powder, polygonatum odoratum (mill.) druce powder and oat bran powder as a composite substrate, inoculated with bacillus ATCC 6633 for aerobic fermentation, under the condition that the first stage fermentation liquor is not inactivated, glucose, pyridoxal phosphate and lactobacillus ATCC 367 are added, and micro-aerobic and low-oxygen fermentation is sequentially carried out, and the composition is prepared through solid-liquid separation, ultrafiltration, concentration and drying. The composition can inhibit alpha-glucosidase activity, promote glucose utilization, improve insulin resistance and increase liver glycogen content, and can be used for preparing products for treating type 2 diabetes and auxiliary regulation of blood sugar.

The invention relates to a metabolic biomarker for malignant transformation of chronic hepatitis B and application of the metabolic biomarker, and belongs to the technical field of biomedicine. The invention provides aldehyde oxidase 1 (AOX1) and its downstream metabolite pyridoxal phosphate (PLP) as metabolic biomarkers for chronic hepatitis B malignant transformation. Through metabonomics and transcriptomics analysis, a'first-rising and second-falling 'dynamic trajectory of PLP rising in a chronic hepatitis B stage and falling in a liver cancer stage is found, and HBx reduces the PLP level by inhibiting AOX1 expression. The marker can be used for preparing a detection reagent, a kit or an integrated device, and dynamic monitoring and early warning of the vicious transformation risk are realized by detecting the AOX1 / PLP level in samples such as serum. The marker has the advantages of being clear in mechanism, high in dynamic relevance, suitable for clinical transformation and the like, and a new means is provided for liver cancer risk monitoring of chronic hepatitis B patients.

The invention discloses an (S)-transaminase mutant and an application of the (S)-transaminase mutant in preparation of (R)-3-([1, 1 '-biphenyl]-4-yl)-2-aminopropyl-1-alcohol. The (S)-transaminase mutant is obtained by mutating wild type (S)-transaminase with the NCBI (National Center of Biotechnology Information) login number of KRF52528.1. According to the (S)-transaminase mutant, pyridoxal phosphate is used as a cofactor, and isopropylamine and 3-([1, 1 '-biphenyl]-4-yl)-2-ketopropyl-1-ol are catalyzed to synthesize (R)-3-([1, 1'-biphenyl]-4-yl)-2-aminopropyl-1-ol. When the (S)-transaminase mutant is used for producing (R)-3-([1, 1 '-biphenyl]-4-yl)-2-aminopropyl-1-ol, the (S)-transaminase mutant has the following advantages: (1) the production process is simple, and the reaction conditions are mild; (2) the (S)-transaminase mutant is high in selectivity, the optical purity of the product is high, and splitting is not needed; (3) the atom utilization rate is high, the production process is environment-friendly, and the green chemistry concept is met; and (4) the product is simple to separate and purify and low in subsequent treatment difficulty.

The invention discloses a D-threonine aldolase mutant and an application of the D-threonine aldolase mutant in preparation of D-configuration beta-hydroxyl-alpha-amino acid. The D-threonine aldolase mutant is obtained by mutating a wild type D-threonine aldolase of which the accession number is WP048940648.1 in the NCBI (National Center of Biotechnology Information). According to the D-threonine aldolase mutant, pyridoxal phosphate and manganese ions are used as cofactors, and glycine, p-methylsulfonyl benzaldehyde, p-nitrobenzaldehyde, 2, 4-dihydroxy benzaldehyde and the like are catalyzed to be condensed to form D-configuration beta-hydroxyl-alpha-amino acid. When the D-threonine aldolase mutant is used for producing D-configuration beta-hydroxyl-alpha-amino acid, the D-threonine aldolase mutant has the following advantages: (1) the production process is simple, and the reaction conditions are mild; (2) the D-threonine aldolase mutant is high in selectivity, the optical purity of the product is high, and resolution is not needed; (3) the atom utilization rate is high, the production process is environment-friendly, and the green chemistry concept is met; and (4) the product is simple to separate and purify and low in subsequent treatment difficulty.