46 results about "Coprinus" patented technology

The invention discloses a straw starter. The straw starter comprises Coprinus sterqulinus, penicillium expansum, trichoderma koningii and Coprinopsis atramentaria. The straw starter can be utilized for microbial fermentation of multiple straws of wheat straws and rice straws, is safe and nontoxic, has strong fermentation condition adaptability, allows a wide temperature range, and can be utilizedfor fermentation at an outdoor temperature of 15 to 35 DEG C. Based on different temperatures, fermentation time is in a range of 20 to 80 days. Straws fermented by the straw starter can be utilized as matrixes of biogas, forage and organic fertilizer.

The invention relates to ultra-Mn2<+>-resistant bacterial laccase, a recombinant vector, recombinant bacteria, an enzymic preparation, a compound enzyme system and a preparation method and an application thereof and belongs to the biotechnical field. The ultra-Mn2<+>-resistant bacterial laccase is originated from bacterial laccase lac1542 of metagenome, and the gene nucleotide sequence of the laccase is as shown in SEQ ID NO.1. The optimum pH of the bacterial laccase taking ABTS as a primer is 4.0; the optimum reaction temperature is 75 DEG C and the bacterial laccase is highly stable at a high temperature; 100mmol/L Mn2<+> still can activate the enzyme. Bacterial laccase, manganese peroxidase and coprinus cinereus peroxidase form the compound enzyme system which can degrade 71.5% of lignin at a low concentration; the decolourization ratio of the bacterial laccase and coprinus cinereus peroxidase compound enzyme system on methyl orange can reach 54.8%. The bacterial laccase provided by the invention can be used in the field of papermaking, dyeing and weaving and environmental protection as a novel enzyme source and has a wide industrial application prospect.

The invention relates to a fungus removing device, in particular to a clamping-free type coprinus encircling cutting apparatus. According to the provided clamping-free type coprinus encircling cuttingapparatus, in order to solve the technical problem that secondary coprinus diseases are likely to explode since the bottoms of pilei are not protected in the process of using existing devices for mechanically aligning the pilei and then manually cleaning away coprinus, a pileus clamping and conveying device, double-plate clamps, a film cutting and providing device and a stipe and pileus separating device are designed; sufficient film is cut by the film cutting and providing device first to wrap pilei through the double-plate clamps, stipes are cut by the stipe and pileus separating device, and finally the pilei are discharged out of the apparatus by the pileus clamping and conveying device. The apparatus achieves the effects of tightly wrapping the pilei to prevent explosion of secondarydiseases caused by spore scattering, ensuring stable cutting of the stipes during encircling cutting under the condition of no fixing device, automatically discharging the pilei from the apparatus, automatically removing coprinus and preventing explosion of secondary coprinus diseases.

The invention relates to a preparation method for a lignocellulose decomposition composite flora and application thereof, discloses a method which can prepare a high-efficiency lignocellulose decomposition composite flora rapidly, simply and conveniently, in particular provides a microbial inoculum for the composting of cow muck and agricultural straw, and solves the problem that the cow muck and the agricultural straw are difficult to compost. The composite flora is formed by mixing fermentation liquor obtained by fermenting a cow muck culture medium and spore suspension of phanerochete chrysosporium and trichoderma sp, rhizopus sp, panaeolus sp and coprinus sp which are separated from the cow muck, and can completely degrade the cow muck and the agricultural straw of lignocellulose. By using the microbial inoculum in the composting of the cow muck and the straw, the fermentation period is as short as only 25 days. The preparation method for the flora is simple and is easy to implement; the flora content in each unit of raw materials is high; and the activity is high.

The invention discloses a fungal laccase mutant PIE5 as well as an expression strain and application thereof, wherein the fungus laccase mutant PIE5 is a laccase mutant obtained by carrying out transformation on Coprinus cinereus laccase LCC 9 by virtue of a directed evolution means, constructing a mutation library and combining three rounds of screening, and the application capability of the laccase can be improved. The optimal catalytic pH of the fungal laccase mutant PIE5 is 8.5 (guaiacol is used as a substrate), and the fungal laccase mutant PIE5 can still play good activity when the pH value reaches 9.5, so that the fungal laccase mutant PIE5 has an application advantage in an alkaline environment, the application field of the fungal laccase is expanded, the fungal laccase mutant PIE5can be directly applied to a textile finishing process to replace stone mill water washing, the pH of wastewater does not need to be adjusted in a wastewater indigo degradation process, improvement of the textile finishing process of the fungal laccase is facilitated, the operation cost is reduced, and the ecological environment is protected.

The invention discloses a preparation method for selenium-enriched Coprinus comatus mycelia, which is characterized in that the preparation method comprises the following steps of: 1) taking Coprinus comatus spawns and culturing the Coprinus comatus spawns in a slant culture medium to obtain Coprinus comatus slant spawns; 2) taking and inoculating the Coprinus comatus slant spawns obtained at thestep (1) into a shake flask culture medium for shaking bed culture to obtain liquid spawns; and 3) taking and inoculating the liquid spawns obtained at the step (2) into a selenium-containing fermentation culture medium for fermentation, stopping fermentation till the fermentation liquid is transparent, the flavor is fragrant, the mycelium biomass is 20-30g/L and the pH is 5-7, and obtaining the selenium-enriched Coprinus comatus mycelia. The invention additionally discloses a preparation method for selenium-enriched Coprinus comatus powder. Since Coprinus comatus is used as carriers to realize the organic transformation of selenium, the biological functions of the Coprinus comatus and the selenium are compensated.

The Coprinus comaus food is produced with Coprinus comaus as main material and salt, vegetable oil and seasonings. Its production process includes preparing material; sorting and washing fresh Coprinus comaus; adding salt to eliminate partial water in fresh Coprinus comaus and slicing; mixing Coprinus comaus with vegetable oil and seasonings; packing, vacuum pumping, and sealing; steaming at 100-110 deg.c for 0.5-2 hr; and aging. It is delicious, rich in nutrients and convenient in eating.

A three-dimensional high-efficiency green intelligent mushroom cultivation box belongs to the field of three-dimensional intelligent cultivation of mushrooms in the original ecological environment imitating the wild environment. On the hard ground and the floor frame, there are cultivation frames evenly placed on the top of the floor frame, and the inner liner pipe of the cultivation frame There is a mushroom growth medium on it. Using the irrigation method that the nutrient solution penetrates from the nutrient supply cavity to the outside to supplement the nutrition of the mushroom culture medium to cultivate morels, puffballs, colorful mushrooms, colorful lotus mushrooms, shiitake mushrooms, white jade mushrooms, mushrooms, Coprinus comatus, Flammulina velutipes, Red fir mushroom, Shimeji mushroom, tea tree mushroom, Ganoderma lucidum, Hericium erinaceus, Pleurotus ostreatus, Tricholoma, Bailing mushroom, Pleurotus eryngii, flower mushroom, crab-flavored mushroom, Xiuzhen mushroom and other mushrooms. The method of cultivating various mushrooms is simple and easy to operate, which is convenient for large-scale and industrialization. Cultivating morels in four seasons ensures the supply of the market in four seasons; the cultivated morels have high yield and good quality, and the commercial value of industrialization is high The mushroom growth medium can repeatedly cultivate mushrooms, which reduces the cultivation cost of mushrooms.

The invention belongs to the technical field of food and food processing, and particularly relates to a nutrient vegetable dumpling and a processing method of the dumpling. The vegetable dumpling consists of a dumpling wrapper and a filling. The dumpling wrapper comprises the following raw materials by weight in grams: 900 g of flour,100 g of puffing corn powder, 1.5 g of sodium alginate and 20 g of egg white liquid; the filling comprises the following raw materials by weight in grams: 400 g of dry flower mushroom, 100 g of fresh coprinus, 100 g of cucumber,100 g of carrot, 100 g of cabbage, 150 g of black fungus, 175 g of onion, 50 g of green pepper, 100 g of fennel seedling, 100 g of peanut protein powder, 1,000 g of water, 50 g of oil, 10 g of salt, 10 g of radix aucklandiae powder and 1 g of dried ginger powder. The nutrient vegetable dumpling has the advantages of being good in taste, and balanced and reasonable in nutrition collocation.

The invention discloses a peptide juice compounded with anti-fatigue peptide and mango juice and a preparation method thereof, which belongs to the technical field of liquid processing and provides a new type of functional peptide juice food for the market. Kiwi peptide powder, seabuckthorn peptide powder, tiger palm peptide powder, Coprinus comatus peptide powder, golden mushroom peptide powder, bolete peptide powder, beet peptide powder, matsutake peptide powder, brown rice peptide powder, blueberry peptide powder, pea peptide powder , Chestnut mushroom peptide powder. These small molecular peptides can ensure the nutrition needed by athletes, have energy, strength and speed in competitions, reduce skeletal muscle damage, and help win championships. These are the main ingredients. Then take polysaccharides, vitamins, trace elements, essential oils, etc. as auxiliary materials. These main materials and auxiliary materials are weighed, mixed, granulated, dried and packaged to make mixed peptide powder. Take the pure mango juice and put it into the fresh-keeping can. According to the ratio of 1:50. Peptide Juice Made. It has the effect of supplementing nutrition.

The invention discloses a feed additive composition, in particular to a feed additive composition for increasing the velvet antler collecting amount. The feed additive composition is prepared from, by weight, 8-10 parts of coprinus sterqulinus, 5-7 parts of morchella crassipes, 5-7 parts of rhaponticum carthamoides, 5-7 parts of maitake mushroom and 2-4 parts of oriental blueberry leaves. The feed additive composition can increase the velvet antler collecting amount.

The present invention relates to a method of designing laccase mutants with improved stability properties, which method is based on the hitherto unknown three-dimensional structure of Coprinus cinereus laccase.

The invention discloses mutation coprinus cinereus peroxidase and the preparing method and application thereof and belongs to the technical fields of genetic engineering and the environment. CIPmt4 is synthesized by means of a gene synthesis site-specific mutagenesis platform, error-prone PCR and resistance screening are conducted three times to obtain the coprinus cinereus peroxidase CIPmt5 containing five amino acid mutations (I49S, V53A, T121A, M166F and Y272F), and the amino acid sequence is shown in SEQ ID NO:3. The optimal reaction temperature of the mutation coprinus cinereus peroxidase is increased to 45 DEG C from 25 DEG C corresponding to the wild type, the optimal reaction pH is increased to 6.5 from the original 5.0, the high-activity reaction pH becomes larger, stability is improved, and the mutation coprinus cinereus peroxidase is more suitable for industrialized application. Except methylene blue, the optimal pH of the mutation coprinus cinereus peroxidase CIPmt5 for degradation of dye amino black, methyl orange, Congo red, aniline blue, crystal violet and bromophenol blue tends to be neutral and alkali, and the degradation ability on the five kinds of dye is remarkably higher than that of wild-type enzyme.

The invention discloses hypoglycemic buckwheat fungus vermicelli and a processing method. The hypoglycemic buckwheat fungus vermicelli is prepared from the raw materials in percentage by weight: 18-22% of tremella, 8-12% of morchella, 18-22% of coprinus, 38-42% of tartary buckwheat, 5% of eggs and 5% of rhodiola. When the hypoglycemic buckwheat fungus vermicelli is eaten, a hypoglycemic effect ona body of an eater with high blood sugar can be produced, and the addition of the tremella, the morchella, the coprinus, the eggs and the rhodiola enables the hypoglycemic buckwheat fungus vermicellito be more delicious in the process of eating, and it is beneficial for people with high blood sugar to eat.

The present invention relates to polyesterases having cutinase and/or suberinase activity obtainable from Coprinus and Trichoderma. The invention further relates to a method for producing the polyesterases, and to polynucleotides, vectors and host cells used therein. The enzymes are useful in hydrolysing cutin, suberin and other polyesters for example in treating agricultural or food raw materials, or wood raw materials, pulp and paper products and waste, and in modifying polyester fibers, or in laundry and dish applications.

The invention provides a method of achieving high-accuracy site-directed gene knock-out in coprinus cinereus. The method includes the steps of: 1) acquiring upstream and downstream sequences of a target knock-out gene through methods of polymerase chain reaction or total gene synthesis, and connecting the upstream and downstream sequences in the opposite direction of the original gene, and performing insertion onto a vector plasmid to construct an annular clone vector; 2) performing linear treatment to the annular clone vector to obtain linear double-chain DNA; and 3) processing the linear double-chain DNA through a 5'-to-3' exonuclease to form a linear 3'-terminal protruded long cohesive end double-chain DNA, thereby converting cells of coprinus cinereus. In the method, through structure and type of a special exogenous DNA, high-accuracy site-directed gene knock-out is achieved in the coprinus cinereus with site-directed gene knock-out accuracy rate reaching 25-70%. The method greatly reduces work load of the site-directed gene knock-out of the coprinus cinereus and has huge application potential in the fields of functional gene researching of coprinus cinereus and construction of a gene engineering bacterial strain.

The invention belongs to the field of microbial technology, and in particular relates to a culture method capable of improving enzymatic activity of laccase. Specifically, the method capable of improving the enzymatic activity of the laccase provided by the invention is achieved through mixed culture of pleurotus of pleurotaceae family and pleurotus or coprinus of agaricaceae family. According to the culture method provided by the invention, by conducting the mixed culture on the two bacteria and through simultaneous inoculation or ordered inoculation, the enzymatic activity of the laccase can be improved by 20-600%.

The invention discloses a traditional Chinese medicine preparation for postpartum care. The traditional Chinese medicine preparation comprises raw materials in parts by weight as follows: 3-8 parts ofherba agrimoniae, 3-8 parts of radix rehmanniae recens, 1-5 parts of herba taxilli, 1-5 parts of rhizoma zingiberis, 3-8 parts of coprinus sporophore, 10-30 parts of fructus hordei germinatus, 1-10 parts of herba leonuri, 3-20 parts of fructus lycii, 5-15 parts of sweet basil herb, 1-5 parts of pterocarpus indicus core, 3-8 parts of leaf of Chinese desmos and 3-8 parts of fructus citri sarcodactylis. The traditional Chinese medicine preparation has the effects of benefiting qi, nourishing blood, cooling blood, stopping bleeding, dispersing blood stasis, relieving pain, resisting bacteria anddiminishing inflammation and has remarkable curative effect, medicine properties are changed or relieved by optimizing compatibility of the traditional Chinese medicines, and toxicity and side effectsof the medicines are eliminated. The traditional Chinese medicine preparation is specially used for postpartum care and has wide indication range, short curative period and remarkable curative effect. The invention further discloses a preparation method of the traditional Chinese medicine preparation. The method is simple in process, high in operability and beneficial to large-scale industrial production.

The invention relates to an antihypelipidemic and antihyperglycemic product prepared from Hymenomucetes and method of preparation, which comprises the steps of, (1) bacterial acquisition, determining bacterial strain of Agaricaceae according to standard of classification, (2) bacterium powder preparing, (3) cytoclasis.

The invention discloses preparation method for a culture medium of an edible fungus. The method includes the following steps: placing a mixture of cottonseed hull and wheat bran, and a water-boiled liquid of broccoli in a container and then uniformly stirring, adding and uniformly stirring glucoamylase after the temperature is adjusted to 30 DEG C-65 DEG C, and then continuing to place for 1-5 h to obtain the culture medium. The culture medium of the edible fungus obtained by utilizing the preparation method can be used for cultivating various edible fungi such as pleurotus eryngii, pleurotusostreatus, and coprinus comatua, is high in biotransformation rate, effectively utilizes resources, and enables the burden of environmental pollution to be reduced. The preparation method also makes the water-boiled liquid of the broccoli turn into treasure, achieves harmless comprehensive utilization, enhances the added value of the broccoli, and has a broad application prospect.

The invention discloses a cultivation bag material used only for Coprinus comatus, which comprises the following components in parts by weight: 5-15 parts of Chinese herbal medicine slag mixture, 25-35 parts of sawdust, 30-50 parts of bagasse, and 20-35 parts of peanut meal part and a sufficient amount of water; wherein the mixture of traditional Chinese medicine residues includes the following traditional Chinese medicine raw materials: Shayuanzi, peach kernel, Eucommia ulmoides, mulberry fruit, Mori tree and hemp seed. In the cultivation bag material of the present invention, Chinese medicinal material fragments are added, which is completely pollution-free, and can also provide specific nutrition for the cultivated Coprinus comatus, so that the cultivated Coprinus comatus varieties are more excellent; Elements make the cultivated Coprinus comatus hyphae grow vigorously, the mushroom body is strong, the mushroom cap is large and thick, the yield is high, and the commodity properties are good; the cultivated Coprinus comatus has the advantages of early hyphae and good fruiting body development, which greatly improves Coprinus comatus has a high yield; sufficient sources of raw materials, simple technology, low cost, high yield, full spore powder, no need to add grain, quick results, and is suitable for widespread application.