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47 results about "Coprinus" patented technology

Coprinus is a small genus of mushroom-forming fungi consisting of Coprinus comatus - the shaggy ink cap (British) or shaggy mane (American) - and several of its close relatives. Until 2001, Coprinus was a large genus consisting of all agaric species in which the lamellae autodigested to release their spores. The black ink-like liquid this creates gave these species their common name "ink cap" (British) or "inky cap" (American).

Straw starter and use of the straw starter in straw fermentation

The invention discloses a straw starter. The straw starter comprises Coprinus sterqulinus, penicillium expansum, trichoderma koningii and Coprinopsis atramentaria. The straw starter can be utilized for microbial fermentation of multiple straws of wheat straws and rice straws, is safe and nontoxic, has strong fermentation condition adaptability, allows a wide temperature range, and can be utilizedfor fermentation at an outdoor temperature of 15 to 35 DEG C. Based on different temperatures, fermentation time is in a range of 20 to 80 days. Straws fermented by the straw starter can be utilized as matrixes of biogas, forage and organic fertilizer.
Owner:北海强兴生物科技有限公司

Ultra-Mn2<+>-resistant bacterial laccase, recombinant vector, recombinant bacteria, enzymic preparation, compound enzyme system and preparation method and application thereof

The invention relates to ultra-Mn2<+>-resistant bacterial laccase, a recombinant vector, recombinant bacteria, an enzymic preparation, a compound enzyme system and a preparation method and an application thereof and belongs to the biotechnical field. The ultra-Mn2<+>-resistant bacterial laccase is originated from bacterial laccase lac1542 of metagenome, and the gene nucleotide sequence of the laccase is as shown in SEQ ID NO.1. The optimum pH of the bacterial laccase taking ABTS as a primer is 4.0; the optimum reaction temperature is 75 DEG C and the bacterial laccase is highly stable at a high temperature; 100mmol / L Mn2<+> still can activate the enzyme. Bacterial laccase, manganese peroxidase and coprinus cinereus peroxidase form the compound enzyme system which can degrade 71.5% of lignin at a low concentration; the decolourization ratio of the bacterial laccase and coprinus cinereus peroxidase compound enzyme system on methyl orange can reach 54.8%. The bacterial laccase provided by the invention can be used in the field of papermaking, dyeing and weaving and environmental protection as a novel enzyme source and has a wide industrial application prospect.
Owner:NANYANG NORMAL UNIV

Clamping-free type coprinus encircling cutting apparatus

ActiveCN110547144APrevent secondary disease outbreaksCultivating equipmentsHorticulture methodsSporeDisease
The invention relates to a fungus removing device, in particular to a clamping-free type coprinus encircling cutting apparatus. According to the provided clamping-free type coprinus encircling cuttingapparatus, in order to solve the technical problem that secondary coprinus diseases are likely to explode since the bottoms of pilei are not protected in the process of using existing devices for mechanically aligning the pilei and then manually cleaning away coprinus, a pileus clamping and conveying device, double-plate clamps, a film cutting and providing device and a stipe and pileus separating device are designed; sufficient film is cut by the film cutting and providing device first to wrap pilei through the double-plate clamps, stipes are cut by the stipe and pileus separating device, and finally the pilei are discharged out of the apparatus by the pileus clamping and conveying device. The apparatus achieves the effects of tightly wrapping the pilei to prevent explosion of secondarydiseases caused by spore scattering, ensuring stable cutting of the stipes during encircling cutting under the condition of no fixing device, automatically discharging the pilei from the apparatus, automatically removing coprinus and preventing explosion of secondary coprinus diseases.
Owner:拜泉县鑫鑫菌业有限公司

Preparation method for lignocellulose decomposition composite flora and application thereof

The invention relates to a preparation method for a lignocellulose decomposition composite flora and application thereof, discloses a method which can prepare a high-efficiency lignocellulose decomposition composite flora rapidly, simply and conveniently, in particular provides a microbial inoculum for the composting of cow muck and agricultural straw, and solves the problem that the cow muck and the agricultural straw are difficult to compost. The composite flora is formed by mixing fermentation liquor obtained by fermenting a cow muck culture medium and spore suspension of phanerochete chrysosporium and trichoderma sp, rhizopus sp, panaeolus sp and coprinus sp which are separated from the cow muck, and can completely degrade the cow muck and the agricultural straw of lignocellulose. By using the microbial inoculum in the composting of the cow muck and the straw, the fermentation period is as short as only 25 days. The preparation method for the flora is simple and is easy to implement; the flora content in each unit of raw materials is high; and the activity is high.
Owner:KUNMING UNIV OF SCI & TECH

A kind of preparation method of selenium-enriched Coprinus comatus mycelium and bacterial powder thereof

ActiveCN102283021AComplementary Biological FunctionsNo infectionHorticultureMicrobiologyFood flavor
The invention discloses a preparation method for selenium-enriched Coprinus comatus mycelia, which is characterized in that the preparation method comprises the following steps of: 1) taking Coprinus comatus spawns and culturing the Coprinus comatus spawns in a slant culture medium to obtain Coprinus comatus slant spawns; 2) taking and inoculating the Coprinus comatus slant spawns obtained at thestep (1) into a shake flask culture medium for shaking bed culture to obtain liquid spawns; and 3) taking and inoculating the liquid spawns obtained at the step (2) into a selenium-containing fermentation culture medium for fermentation, stopping fermentation till the fermentation liquid is transparent, the flavor is fragrant, the mycelium biomass is 20-30g / L and the pH is 5-7, and obtaining the selenium-enriched Coprinus comatus mycelia. The invention additionally discloses a preparation method for selenium-enriched Coprinus comatus powder. Since Coprinus comatus is used as carriers to realize the organic transformation of selenium, the biological functions of the Coprinus comatus and the selenium are compensated.
Owner:GUANGDONG MEIWEIXIAN FLAVORING & FOOD

Psilocybe laccase with anti-tumor cell proliferation activity and preparation method thereof

The invention discloses coprinus comatus laccase with activity of inhibiting tumor cell proliferation and a preparation method thereof. The coprinus comatus laccase provided in the invention is prepared by the following steps: 1) breaking up cells; 2) collecting a crude extract of proteins; 3) separating and purifying laccase from the crude extract of proteins collected in the step 2), wherein the obtained laccase is an extract of coprinus comatus, the molecular weight of the laccase is 64KD, and N terminal sequence of the laccase is described by sequence 1 in a sequence table. The coprinus comatus extract obtained through the preparation method in the invention has an obvious inhabiting effect on cancer cell proliferation and HIV reverse transcriptase activity and has an important value in the field of research on novel anti-cancer and anti-AIDS medicines.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1

Making method of coprinus comatus meat sauce

The invention discloses a method for preparing Coprinus comatus meat sauce, which is characterized in that 20-40 parts of fresh pork, 10-20 parts of fresh Coprinus comatus, 8-10 parts of vegetable oil, 15-30 parts of refined vegetable oil, 3-30 parts of soybean paste 5 parts, 2-5 parts of salt, 4-8 parts of white sugar, 1-2 parts of balsamic vinegar, 2-2.5 parts of chili noodles, 1-2 parts of spices, 1-2 parts of fresh onion, 3-5 parts of minced garlic, 2-4 parts of ginger powder, 3-5 parts of pork paste, 0.3-1 part of water, the product of the present invention has strong flavor, easy to eat, store and carry, and is suitable for collocation with dumplings, boiled meat, seafood, cold vegetables, vermicelli and other foods edible.
Owner:XUZHOU TULIP AGRI DEV CO LTD

Coprinus comaus food and its production process

The Coprinus comaus food is produced with Coprinus comaus as main material and salt, vegetable oil and seasonings. Its production process includes preparing material; sorting and washing fresh Coprinus comaus; adding salt to eliminate partial water in fresh Coprinus comaus and slicing; mixing Coprinus comaus with vegetable oil and seasonings; packing, vacuum pumping, and sealing; steaming at 100-110 deg.c for 0.5-2 hr; and aging. It is delicious, rich in nutrients and convenient in eating.
Owner:秦怀跃

Stereo, efficient, green and intelligent mushroom culture box

A three-dimensional high-efficiency green intelligent mushroom cultivation box belongs to the field of three-dimensional intelligent cultivation of mushrooms in the original ecological environment imitating the wild environment. On the hard ground and the floor frame, there are cultivation frames evenly placed on the top of the floor frame, and the inner liner pipe of the cultivation frame There is a mushroom growth medium on it. Using the irrigation method that the nutrient solution penetrates from the nutrient supply cavity to the outside to supplement the nutrition of the mushroom culture medium to cultivate morels, puffballs, colorful mushrooms, colorful lotus mushrooms, shiitake mushrooms, white jade mushrooms, mushrooms, Coprinus comatus, Flammulina velutipes, Red fir mushroom, Shimeji mushroom, tea tree mushroom, Ganoderma lucidum, Hericium erinaceus, Pleurotus ostreatus, Tricholoma, Bailing mushroom, Pleurotus eryngii, flower mushroom, crab-flavored mushroom, Xiuzhen mushroom and other mushrooms. The method of cultivating various mushrooms is simple and easy to operate, which is convenient for large-scale and industrialization. Cultivating morels in four seasons ensures the supply of the market in four seasons; the cultivated morels have high yield and good quality, and the commercial value of industrialization is high The mushroom growth medium can repeatedly cultivate mushrooms, which reduces the cultivation cost of mushrooms.
Owner:巫忠胜

Nutrient vegetable dumpling and processing method thereof

The invention belongs to the technical field of food and food processing, and particularly relates to a nutrient vegetable dumpling and a processing method of the dumpling. The vegetable dumpling consists of a dumpling wrapper and a filling. The dumpling wrapper comprises the following raw materials by weight in grams: 900 g of flour,100 g of puffing corn powder, 1.5 g of sodium alginate and 20 g of egg white liquid; the filling comprises the following raw materials by weight in grams: 400 g of dry flower mushroom, 100 g of fresh coprinus, 100 g of cucumber,100 g of carrot, 100 g of cabbage, 150 g of black fungus, 175 g of onion, 50 g of green pepper, 100 g of fennel seedling, 100 g of peanut protein powder, 1,000 g of water, 50 g of oil, 10 g of salt, 10 g of radix aucklandiae powder and 1 g of dried ginger powder. The nutrient vegetable dumpling has the advantages of being good in taste, and balanced and reasonable in nutrition collocation.
Owner:王国强

Peptide juice prepared by compounding mango juice with peptides having effects of resisting fatigue and increasing physical strength, and preparation method of peptide juice

The invention discloses a peptide juice compounded with anti-fatigue peptide and mango juice and a preparation method thereof, which belongs to the technical field of liquid processing and provides a new type of functional peptide juice food for the market. Kiwi peptide powder, seabuckthorn peptide powder, tiger palm peptide powder, Coprinus comatus peptide powder, golden mushroom peptide powder, bolete peptide powder, beet peptide powder, matsutake peptide powder, brown rice peptide powder, blueberry peptide powder, pea peptide powder , Chestnut mushroom peptide powder. These small molecular peptides can ensure the nutrition needed by athletes, have energy, strength and speed in competitions, reduce skeletal muscle damage, and help win championships. These are the main ingredients. Then take polysaccharides, vitamins, trace elements, essential oils, etc. as auxiliary materials. These main materials and auxiliary materials are weighed, mixed, granulated, dried and packaged to make mixed peptide powder. Take the pure mango juice and put it into the fresh-keeping can. According to the ratio of 1:50. Peptide Juice Made. It has the effect of supplementing nutrition.
Owner:TANGSHAN SHISAN TAIBAO BIOTECH CO LTD +2

Feed additive composition for increasing velvet antler collecting amount

InactiveCN105995062AImprove the function of the digestive systemPromote absorptionAnimal feeding stuffAnimal scienceFeed additive
The invention discloses a feed additive composition, in particular to a feed additive composition for increasing the velvet antler collecting amount. The feed additive composition is prepared from, by weight, 8-10 parts of coprinus sterqulinus, 5-7 parts of morchella crassipes, 5-7 parts of rhaponticum carthamoides, 5-7 parts of maitake mushroom and 2-4 parts of oriental blueberry leaves. The feed additive composition can increase the velvet antler collecting amount.
Owner:唐魏

A kind of fungal laccase mutant pie5 and its expression strain and application

The invention discloses a fungal laccase mutant PIE5 and its expression strain and application, wherein the fungal laccase mutant PIE5 transforms Coprinus cinerea laccase Lcc9 by means of directed evolution, constructs a mutation library, combines three rounds of Screening method to obtain laccase mutants to improve application ability of laccase. The fungal laccase mutant PIE5 of the present invention has an optimum catalytic pH of 8.5 (guaiacol is the substrate), and can still exert good activity when the pH reaches 9.5, so the fungal laccase PIE5 has an alkaline environment by comparison. Application advantages broaden the application field of fungal laccase, which can be directly applied to textile finishing process instead of stone mill washing, and there is no need to adjust the pH of wastewater in the degradation of wastewater indigo, which is conducive to improving the textile finishing process of fungal laccase, reducing Operating costs and protecting the ecological environment.
Owner:ANHUI UNIVERSITY

Laccase mutants

The present invention relates to a method of designing laccase mutants with improved stability properties, which method is based on the hitherto unknown three-dimensional structure of Coprinus cinereus laccase.
Owner:NOVOZYMES AS

Mutation coprinus cinereus peroxidase and preparing method and application thereof

The invention discloses mutation coprinus cinereus peroxidase and the preparing method and application thereof and belongs to the technical fields of genetic engineering and the environment. CIPmt4 is synthesized by means of a gene synthesis site-specific mutagenesis platform, error-prone PCR and resistance screening are conducted three times to obtain the coprinus cinereus peroxidase CIPmt5 containing five amino acid mutations (I49S, V53A, T121A, M166F and Y272F), and the amino acid sequence is shown in SEQ ID NO:3. The optimal reaction temperature of the mutation coprinus cinereus peroxidase is increased to 45 DEG C from 25 DEG C corresponding to the wild type, the optimal reaction pH is increased to 6.5 from the original 5.0, the high-activity reaction pH becomes larger, stability is improved, and the mutation coprinus cinereus peroxidase is more suitable for industrialized application. Except methylene blue, the optimal pH of the mutation coprinus cinereus peroxidase CIPmt5 for degradation of dye amino black, methyl orange, Congo red, aniline blue, crystal violet and bromophenol blue tends to be neutral and alkali, and the degradation ability on the five kinds of dye is remarkably higher than that of wild-type enzyme.
Owner:NANYANG NORMAL UNIV

Hypoglycemic buckwheat fungus vermicellis and processing method

The invention discloses hypoglycemic buckwheat fungus vermicelli and a processing method. The hypoglycemic buckwheat fungus vermicelli is prepared from the raw materials in percentage by weight: 18-22% of tremella, 8-12% of morchella, 18-22% of coprinus, 38-42% of tartary buckwheat, 5% of eggs and 5% of rhodiola. When the hypoglycemic buckwheat fungus vermicelli is eaten, a hypoglycemic effect ona body of an eater with high blood sugar can be produced, and the addition of the tremella, the morchella, the coprinus, the eggs and the rhodiola enables the hypoglycemic buckwheat fungus vermicellito be more delicious in the process of eating, and it is beneficial for people with high blood sugar to eat.
Owner:GUANGXI NORMAL UNIV FOR NATITIES

Novel esterases and their use

The present invention relates to polyesterases having cutinase and / or suberinase activity obtainable from Coprinus and Trichoderma. The invention further relates to a method for producing the polyesterases, and to polynucleotides, vectors and host cells used therein. The enzymes are useful in hydrolysing cutin, suberin and other polyesters for example in treating agricultural or food raw materials, or wood raw materials, pulp and paper products and waste, and in modifying polyester fibers, or in laundry and dish applications.
Owner:VALTION TEKNILLINEN TUTKIMUSKESKUS

Traditional Chinese medicine for treating varicosity

The invention relates to a traditional Chinese medicine for treating varicose veins, which is prepared from the following medicinal materials in proportion by weight: 8 parts of safflower, 8 parts of Sanqi, 12 parts of angelica, 8 parts of frankincense, 8 parts of myrrh, 6 parts of Fangfeng, Qianjin 5 parts of pluck, 5 parts of bird sparrow, 6 parts of Coprinus dungensis, 6 parts of scorpion grass, 4 parts of Feilian, 4 parts of hive, and 4 parts of licorice. Experiments prove that the present invention can safely and effectively treat varicose veins.
Owner:王秀红 +1

Method of achieving high-accuracy site-directed gene knock-out in coprinus cinereus

The invention provides a method of achieving high-accuracy site-directed gene knock-out in coprinus cinereus. The method includes the steps of: 1) acquiring upstream and downstream sequences of a target knock-out gene through methods of polymerase chain reaction or total gene synthesis, and connecting the upstream and downstream sequences in the opposite direction of the original gene, and performing insertion onto a vector plasmid to construct an annular clone vector; 2) performing linear treatment to the annular clone vector to obtain linear double-chain DNA; and 3) processing the linear double-chain DNA through a 5'-to-3' exonuclease to form a linear 3'-terminal protruded long cohesive end double-chain DNA, thereby converting cells of coprinus cinereus. In the method, through structure and type of a special exogenous DNA, high-accuracy site-directed gene knock-out is achieved in the coprinus cinereus with site-directed gene knock-out accuracy rate reaching 25-70%. The method greatly reduces work load of the site-directed gene knock-out of the coprinus cinereus and has huge application potential in the fields of functional gene researching of coprinus cinereus and construction of a gene engineering bacterial strain.
Owner:ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Culture method capable of improving enzymatic activity of laccase

InactiveCN106591149AIncreased activity of laccaseFungiOxidoreductasesMixed cultureMicroorganism
The invention belongs to the field of microbial technology, and in particular relates to a culture method capable of improving enzymatic activity of laccase. Specifically, the method capable of improving the enzymatic activity of the laccase provided by the invention is achieved through mixed culture of pleurotus of pleurotaceae family and pleurotus or coprinus of agaricaceae family. According to the culture method provided by the invention, by conducting the mixed culture on the two bacteria and through simultaneous inoculation or ordered inoculation, the enzymatic activity of the laccase can be improved by 20-600%.
Owner:CHANGSHU JINDA TECH CO LTD

Traditional Chinese medicine preparation for postpartum care and preparation method of traditional Chinese medicine preparation

The invention discloses a traditional Chinese medicine preparation for postpartum care. The traditional Chinese medicine preparation comprises raw materials in parts by weight as follows: 3-8 parts ofherba agrimoniae, 3-8 parts of radix rehmanniae recens, 1-5 parts of herba taxilli, 1-5 parts of rhizoma zingiberis, 3-8 parts of coprinus sporophore, 10-30 parts of fructus hordei germinatus, 1-10 parts of herba leonuri, 3-20 parts of fructus lycii, 5-15 parts of sweet basil herb, 1-5 parts of pterocarpus indicus core, 3-8 parts of leaf of Chinese desmos and 3-8 parts of fructus citri sarcodactylis. The traditional Chinese medicine preparation has the effects of benefiting qi, nourishing blood, cooling blood, stopping bleeding, dispersing blood stasis, relieving pain, resisting bacteria anddiminishing inflammation and has remarkable curative effect, medicine properties are changed or relieved by optimizing compatibility of the traditional Chinese medicines, and toxicity and side effectsof the medicines are eliminated. The traditional Chinese medicine preparation is specially used for postpartum care and has wide indication range, short curative period and remarkable curative effect. The invention further discloses a preparation method of the traditional Chinese medicine preparation. The method is simple in process, high in operability and beneficial to large-scale industrial production.
Owner:FOSHAN UNIVERSITY

Preparation method for culture medium of edible fungus

The invention discloses preparation method for a culture medium of an edible fungus. The method includes the following steps: placing a mixture of cottonseed hull and wheat bran, and a water-boiled liquid of broccoli in a container and then uniformly stirring, adding and uniformly stirring glucoamylase after the temperature is adjusted to 30 DEG C-65 DEG C, and then continuing to place for 1-5 h to obtain the culture medium. The culture medium of the edible fungus obtained by utilizing the preparation method can be used for cultivating various edible fungi such as pleurotus eryngii, pleurotusostreatus, and coprinus comatua, is high in biotransformation rate, effectively utilizes resources, and enables the burden of environmental pollution to be reduced. The preparation method also makes the water-boiled liquid of the broccoli turn into treasure, achieves harmless comprehensive utilization, enhances the added value of the broccoli, and has a broad application prospect.
Owner:祁海杰

A kind of comprehensive prevention and control method of Coprinus comatus disease

The invention relates to a comprehensive prevention and control method for coprinus comatus diseases. The comprehensive prevention and control method comprises the steps of (1) selecting the varieties; (2) preparing cultivation bags; (3) disinfecting a coprinus comatus growth shed, and taking insect prevention measures; (4) preparing covering soil; (5) performing non-bag-removal cultivation through the covering soil; (6) managing coprinus comatus growth. According to the comprehensive prevention and control method for the coprinus comatus diseases, the shortcoming that the bags are required to be removed in the conventional coprinus comatus cultivation process is overcome, so that cross infection among the removed cultivation bags is avoided, and local control on the diseases is facilitated; furthermore, due to the disinfection control on a transmission way, the application amount of a chemical insecticide in the coprinus comatus cultivation process is greatly reduced; therefore, the pesticide residue of a product is lowered, and the quality of edible mushrooms is greatly improved.
Owner:INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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