A kind of preparation method of selenium-enriched Coprinus comatus mycelium and bacterial powder thereof

The technology of Agaric agaricus and Agaricus agaricus is applied in the fields of botanical equipment and methods, horticulture, application, etc., and can solve the problems of lack of selenium-enriched Mycelium of Agaricus agaricus and research on the preparation method of mushroom powder, and the like, Achieve the effect of easy mechanized production, no bacterial infection, stable biomass and selenium enrichment rate

Active Publication Date: 2011-12-21
GUANGDONG MEIWEIXIAN FLAVORING & FOOD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research and development of selenium-enriched edible fungus as an organic selenium product has attracted much

Method used

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  • A kind of preparation method of selenium-enriched Coprinus comatus mycelium and bacterial powder thereof
  • A kind of preparation method of selenium-enriched Coprinus comatus mycelium and bacterial powder thereof
  • A kind of preparation method of selenium-enriched Coprinus comatus mycelium and bacterial powder thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) The slant culture medium was prepared according to the following formula. After sterilization, the activated Coprinus trichocephalum species was inoculated, and cultivated at a temperature of 28° C. until the slant surface was covered with fresh Coprinus trichocephalum.

[0039] The formula of the slant medium is: 200g potatoes, 15g glucose, 2g peptone, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 1g, VB 1 0.01g, 20g agar, 1000mL distilled water, natural pH. Wherein, what potato adopts is potato aqueous extract.

[0040] (2) Prepare shake flask culture medium according to the following formula, after sterilization, pick 0.5cm 2 The hyphae of Coprinus comatus slant strains with the colony area were inoculated into the Erlenmeyer shaker flask culture medium, the shaker speed was 100r / min, the temperature was 25°C, the liquid volume was 75mL / 250mL, and cultured for 3 days to obtain liquid strains.

[0041] Shake flask medium formula: 200g potatoes, 30g glucose, 4g peptone, KH 2...

Embodiment 2

[0046] (1) Prepare the slant culture medium according to the following formula, then inoculate the activated Coprinus trichocephalum species after sterilization, and cultivate at a temperature of 25°C until the slant is covered with fresh Coprinus trichocephalum.

[0047] The formula of the slant medium is: 200g potatoes, 15g glucose, 2g peptone, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 1g, VB 1 0.01g, 20g agar, 1000mL distilled water, natural pH. Wherein, what potato adopts is potato aqueous extract.

[0048] (2) Prepare shake flask culture medium according to the following formula, after sterilization, pick 0.75cm 2 The mycelium of the slant strain with the colony area was inoculated into the Erlenmeyer shaker flask culture medium, the shaker speed was 150r / min, the temperature was 28°C, the liquid volume was 100mL / 250mL, and cultured for 4 days to obtain the liquid strain.

[0049] Shake flask medium formula: 200g potatoes, 30g glucose, 4g peptone, KH 2 PO 4 2g, MgSO 4 ·7H 2 ...

Embodiment 3

[0054] (1) Prepare the slant culture medium according to the following formula, then inoculate the activated Coprinus trichocephalum species after sterilization, and cultivate at a temperature of 30°C until the slant is covered with fresh Coprinus trichocephalum.

[0055] The formula of the slant medium is: 200g potatoes, 15g glucose, 2g peptone, KH 2 PO 4 2g, MgSO 4 ·7H 2 O 1g, VB 1 0.01g, 20g agar, 1000mL distilled water, natural pH. Wherein, what potato adopts is potato aqueous extract.

[0056] (2) Prepare shake flask culture medium according to the following formula, after sterilization, pick 1cm 2 The hyphae with the colony area were inoculated into the culture medium of the Erlenmeyer flask shaker, the shaker speed was 100r / min, the temperature was 28°C, the liquid volume was 90mL / 250mL, and cultured for 4 days to obtain liquid strains.

[0057] Shake flask medium formula: 200g potatoes, 30g glucose, 4g peptone, KH 2 PO 4 2g, MgSO 4 ·7H 2 O1g, VB 1 0.01g, dis...

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Abstract

The invention discloses a preparation method for selenium-enriched Coprinus comatus mycelia, which is characterized in that the preparation method comprises the following steps of: 1) taking Coprinus comatus spawns and culturing the Coprinus comatus spawns in a slant culture medium to obtain Coprinus comatus slant spawns; 2) taking and inoculating the Coprinus comatus slant spawns obtained at thestep (1) into a shake flask culture medium for shaking bed culture to obtain liquid spawns; and 3) taking and inoculating the liquid spawns obtained at the step (2) into a selenium-containing fermentation culture medium for fermentation, stopping fermentation till the fermentation liquid is transparent, the flavor is fragrant, the mycelium biomass is 20-30g/L and the pH is 5-7, and obtaining the selenium-enriched Coprinus comatus mycelia. The invention additionally discloses a preparation method for selenium-enriched Coprinus comatus powder. Since Coprinus comatus is used as carriers to realize the organic transformation of selenium, the biological functions of the Coprinus comatus and the selenium are compensated.

Description

technical field [0001] The invention relates to a method for preparing selenium-enriched Coprinus comatus mycelium, and also relates to a method for preparing selenium-enriched Coprinus comatus powder. Background technique [0002] Selenium (Se) is an essential trace element for the human body and has many important physiological functions. Insufficient selenium intake is related to the occurrence of many diseases such as Keshan disease, Kaschin-Beck disease, cardiovascular disease, and tumors. The human body obtains selenium mainly from food. Food comes from agricultural products, and agricultural products absorb selenium from the soil. Selenium deficiency in soil is a widespread problem in the world. The natural selenium content of the earth's soil is generally only 0.1-2.0 μg / g. The average is 0.2 μg / g. Our country is just in the selenium deficiency zone in the world. At present, there are two main types of selenium supplements, one is inorganic selenium, such as sodium...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 崔鹏举余杰杨明泉贾爱娟覃焕达
Owner GUANGDONG MEIWEIXIAN FLAVORING & FOOD
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