13 results about "Coprinus comatus" patented technology

The invention belongs to the technical field of biological medicines, and particularly relates to a nutritional composition as well as a preparation method, application and a product thereof. The nutritional composition provided by the invention consists of nicotinamide, B vitamins and a fungal polysaccharide compound, the vitamin B consists of vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid; the fungal polysaccharide compound is composed of a lucid ganoderma extract, a mushroom extract, an agaricus blazei murill extract, a hericium erinaceus extract, a coprinus comatus extract, a grifola frondosa extract and a cordyceps militaris extract. Western medicine source substances (nicotinamide and B vitamins) and traditional Chinese medicine source substances (fungal polysaccharide compounds) are combined, and the nutrition composition is prepared through combination of the Chinese medicine and the western medicine. The nutritional composition has remarkable effects of enhancing immunity, delaying senescence, resisting fatigue and resisting oxidation, and shows extremely wide application prospects in the field of preparation of health-care products, medicines and cosmetics.

The invention discloses a bactericide composition for preventing and treating earthen archaeological site mushroom diseases and application of the bactericide composition, triazole bactericides and methoxyl acrylate bactericides are used as active ingredients and compounded with a synergist, the mass ratio of triazole to azoxystrobin is 1: 1-1: 9, the content of the synergist is 0.01%-0.1% of the total mass, the content of the methoxyl acrylate bactericides is 0.01%-0.1% of the total mass, and the content of the methoxyl acrylate bactericides is 0.01%-0.1% of the total mass. The composition shows a remarkable synergistic effect, the FIC index is smaller than or equal to 0.5, and the composition has an efficient killing effect on spores, hyphae and sporocarps of common mushrooms in earthen ruins. The composition ensures safety to a ruins body material, shows a color difference value [delta] E < 3, a penetration depth greater than 5 cm, and maintains long-term stability in a humid environment for at least 12 months. The bactericide formula is systematically optimized for earthen ruins mushroom diseases for the first time, and various macro fungi including coprinus brachypoda, coprinus comatus, pleurotus ostreatus and pleurotus citrinopileatus are effectively prevented and treated. Through laboratory screening, synergistic effect testing, permeability and safety evaluation and field application verification, the composition has an excellent prevention and treatment effect.

The present application relates to the technical field of fermentation engineering, and particularly relates to a method for producing organic fertilizer by thermophilic fermentation of mixed compost of rotten citrus fruits and mushroom residue. The method comprises the following steps: (1) crushing and mixing rotten citrus fruits and mushroom residue, adjusting the initial moisture content to 60-65%, and adding lime to obtain mixed materials; (2) adding bacillus velezensis bacterial liquid to the mixed materials obtained in step (1) for composting; (3) when the temperature of the composting pile drops to 35 DEG C, adding coprinus comatus liquid for further composting. The present application uses rotten citrus fruits and mushroom residue as raw materials, and realizes efficient and collaborative resource utilization of the two types of agricultural waste by optimizing the ratio and inoculating exogenous bacterial agents in stages. The method can solve the environmental pollution problem caused by the discarding of rotten fruits in citrus-producing areas from the source, and provides a high-value outlet for the by-products of the mushroom industry.

The invention relates to a preparation method of coprinus comatus selenium polysaccharide. Belongs to the field of pharmacy, and the international patent classification is C08B 37 / 00. The invention relates to a coprinus comatus selenium polysaccharide fine product, which is prepared by the following steps: crushing selenium-rich coprinus comatus mycelium obtained by deep liquid fermentation, sieving, carrying out ultrasonic-assisted extraction, ethanol precipitation, staying overnight at 4 DEG C, centrifuging, discarding supernatant, dissolving the precipitate in water, and deproteinizing by a Sevag method. And concentrating the supernatant, precipitating with ethanol, centrifuging to remove the supernatant, and drying to obtain a coprinus comatus selenium polysaccharide crude product. And purifying the coprinus comatus selenium polysaccharide crude product through ion exchange chromatography and gel filtration chromatography to obtain a coprinus comatus selenium polysaccharide refined product Se-CMP. The obtained coprinus comatus selenium polysaccharide is applied to preparation of drugs for protecting vascular endothelial cells and resisting cardiovascular and cerebrovascular system related diseases such as atherosclerosis.

The invention discloses a directional preparation method of a coprinus comatus protein peptide Maillard reaction product with salt reducing and saltiness increasing capabilities. The directional preparation method comprises the following steps: S1, pretreatment; s2, raw material endogenous enzyme inactivation; s3, first-step enzymolysis: adding cellulase for enzymolysis; s4, performing enzyme deactivation treatment; s5, second-step enzymolysis: adding flavourzyme and aminopeptidase for enzymolysis; s6, performing enzyme deactivation treatment; s7, preparing supernate of the enzymatic hydrolysate; s8, preparation of a Maillard reaction product: adding D-xylose and L-cysteine to carry out a Maillard thermal reaction, and obtaining the coprinus comatus protein peptide Maillard reaction product after the reaction; wherein the addition amount of the D-xylose is 10-12% of the solid content of the enzymatic hydrolysate, and the addition amount of the L-cysteine is 6-13% of the solid content of the enzymatic hydrolysate. According to the method, cellulase, flavourzyme and aminopeptidase are selected to perform two-step hydrolysis on the coprinus comatus, then the Maillard reaction is performed, the ratio of D-xylose to L-cysteine and the reaction time are regulated and controlled, freshness and saltiness are increased to the maximum extent, and the fragrance or meat fragrance of the coprinus comatus is reserved.

The invention discloses a corn softening method based on composite biochemical synergistic treatment, and relates to the technical field of food processing, and the method comprises the following steps: raw material pretreatment: soaking corn kernels in cold water at 10-15 DEG C for 10-14 hours, and carrying out salting-out treatment according to the proportion that 0.4-0.6 kg of edible salt is added into 100 kg of corn; the biological-chemical synergistic treatment comprises the following steps: a) compound alkali treatment: adding edible sodium carbonate accounting for 0.4-0.6% of the dry weight of the corn, adding food-grade baking soda accounting for 0.3-0.7% of the dry weight of the corn, mixing, and maintaining the pH value at 8.5-9.2 for 1-2 hours; b) compound enzyme treatment: 0.002%-0.005% of cellulase, 0.001%-0.003% of hemicellulase and 0.0005%-0.0015% of lignin peroxidase are synchronously added; and nicotinic acid release: carrying out alkali treatment on 0.5 jin / 100 jin of corn to destroy combined nicotinic acid, and further releasing vitamin B groups by combining microbial fermentation pine fungus spores and coprinus comatus enzyme to improve the biological accessibility.

The invention discloses a method for screening odorant substances with sodium reduction potential in edible mushroom Maillard products. The method comprises the following steps: firstly, preparing a coprinus comatus protein peptide Maillard reaction product; the method comprises the following steps: extracting volatile components of the salty and fragrant flavor compounds, respectively carrying out vacuum concentration, preliminarily establishing the association between the flavor and the basic taste by adopting a GC-MS / O-TA combined technology, and screening the potential salty and fragrant flavor compounds. Carrying out gradient dilution on the concentrated solution to obtain diluents with a series of concentrations, analyzing the odor active compounds in the system by adopting a GC-MS / O technology, and recording dilution factors (FD) of the odor active compounds; selecting potential salty and fragrant compounds, and calculating an odor activity value (OAVs); the key salty and fragrant compounds in the system are determined by integrating the FD and OAVs of the potential salty and fragrant compounds, and the salty increasing action mechanism of the salty and fragrant compounds is verified from the molecular level. The problems that in the prior art, the false positive of a screening result is high, key flavor perception characteristics are difficult to capture, and the analysis process is opaque are solved, and a more reliable salty taste enhancing substance screening strategy with a clear mechanism is provided.

The traditional Chinese medicine composition comprises a coprinus comatus extract and a traditional Chinese medicine extract, and the traditional Chinese medicine extract is prepared from mulberry leaves, cyclocarya paliurus leaves, lotus leaves and corn stigma; according to the invention, coprinus comatus is subjected to a sectional crushing process, the particle size range of sporocarp crushing is strictly controlled, and 20-40% of 200-400-mesh coprinus comatus fine powder and 60-80% of an extract extracted from 5-20-mesh coprinus comatus are uniformly mixed in the mixing and drying steps; it is accidentally found that the whole extraction effect on the hypoglycemic effect is better than that of coarse powder with the particle size of 5-20 meshes and fine powder with the particle size of 200-400 meshes.

The present invention provides a composition for improving the barrier function of the intestinal tract. The invention relates to a composition for improving the barrier function of the intestinal tract. The traditional Chinese medicine composition is prepared from urtica heterophyllus, purple perilla, dandelion, golden camellia, codonopsis pilosula, sophora japonica, cistanche, radix angelicae, radix puerariae, ginseng, roselle, tea, chrysanthemum, yam, sea-buckthorn, sambucus nigra, olive, lycium ruthenicum, chenopodium quinoa, radish, grifola frondosa, tartary buckwheat and coprinus comatus. And at least one compound selected from the group consisting of blackberry and raspberry and / or at least one compound selected from the group consisting of phenethyl isothiocyanate and thymoquinone.

The present invention belongs to the technical field of polysaccharide extraction, and discloses a preparation method and application of Coprinus comatus polysaccharide extracted by subcritical water. The preparation method comprises drying Coprinus comatus at 50°C, crushing and passing through a 50-mesh sieve, and storing in a cool and dry place; placing in a subcritical water extraction device and extracting three times; cooling the extracted extract and centrifuging it at 3000r·min‑1 for 10min. The supernatant obtained by centrifugation is added with ethanol, stirred and allowed to stand for 24h, and then centrifuged at 3000r·min‑1 for 10min after standing, and the precipitate is freeze-dried to obtain a Coprinus comatus polysaccharide extract. The preparation method provided by the present invention is simple to operate, has a high extraction rate, greatly saves extraction time, and the Coprinus comatus polysaccharide extract extracted by the present invention has a good ability to inhibit the proliferation of HCT‑116 and A431 cells. It can be applied in anti-tumor activity.

The invention discloses an indoor coprinus comatus planting and fruiting device which comprises a fruiting frame arranged in a planting shed, the fruiting frame is longitudinally divided into multiple layers, and multiple sets of fruiting frames are evenly arranged on each layer; the fruiting frame is of a cuboid structure with the peripheral side faces closed, a plurality of drainage and ventilation holes are formed in the bottom of the fruiting frame, and the bottom of an inner cavity of the fruiting frame is filled with thick nutrient slurry. The nutrient slurry formula is formed by blending granular clay or sandy soil and nutrient water, the thick nutrient slurry is filled with a round fungus bag, the round fungus bag is a coprinus comatus strain, and the round fungus bag is covered with a granular clay layer; the position, 1 cm below the horizontal diameter line, of the circular fungus bag is immersed in the mud. The indoor coprinus comatus planting and fruiting device is reasonable in structure, accurate in nutrition supply, excellent in ventilation and drainage performance, good in fruiting guidance quality and convenient for standardized operation.

This invention discloses a laccase mutant and its application in the oxidative transformation of aflatoxin. This invention uses *Coprinus comatus* (Grey-capped Coprinus) as an example. Coprinopsis cinerea Using laccase as the starting enzyme, structure-activity relationship analysis was conducted through molecular docking, and mutations were designed. After inducing expression in engineered bacteria containing the mutant gene, a mutant laccase Lcc5-T485E with improved detoxification efficiency was obtained through screening. When using the same protein content, the mutant Lcc5-T485E showed a significant improvement in AFB1 conversion efficiency compared to Lcc5, indicating its potential application value in the oxidative conversion of aflatoxin.

This invention discloses a laccase mutant and its application in the oxidative conversion of daidzein to prepare polyflavonoids. This invention uses *Coprinus comatus* (Grey-capped Coprinus) as a starting material. Coprinopsis cinerea Using laccase Lcc5 as the starting enzyme, molecular docking was used to analyze its positional structure and design mutations. The mutant gene was synthesized, and a recombinant expression engineered bacterium containing the mutant gene was constructed. After induction of expression in the engineered bacterium containing the mutant gene, the Lcc5 mutant Lcc5-T485E was obtained through isolation and purification. This mutant showed improved specific enzyme activity and ethanol tolerance, increasing the conversion rate of daidzein by approximately 1.26 times under the same enzyme activity. The antioxidant activity, enzyme-inhibiting activity, and antibacterial activity of the obtained transformation product were also significantly enhanced. This mutant enzyme has potential application value in the oxidative conversion of daidzein to improve its biological value.