30 results about "Daidzein" patented technology

The invention belongs to the field of biosynthesis, and particularly relates to saccharomyces cerevisiae engineering bacteria for producing psoralen as well as a construction method and application of the saccharomyces cerevisiae engineering bacteria. The saccharomyces cerevisiae engineering bacteria comprise a yield increasing path of DMAPP, a synthetic path of naringenin, a synthetic path of daidzein and a synthetic path of psoralen; the yield increasing approach of the DMAPP is to express mvaE, mvaS, ERG12, ERG8, ERG19 and IDI1 on a saccharomyces cerevisiae strain WAT11, and to express mvaE, mvaS, ERG12, ERG8, ERG19 and IDI1 on a saccharomyces cerevisiae strain WAT11. The synthetic route of the naringenin is that PAL, 4CL, CHS and CHI are expressed in Saccharomyces cerevisiae with yield increased by dimethyl allyl pyrophosphoric acid; the synthetic route of daidzein is that CHR, IFS and HID are expressed in saccharomyces cerevisiae synthesized by naringenin; the synthetic route of the psoralen is that PT and OC are expressed in saccharomyces cerevisiae synthesized by daidzein.

This invention provides a method for preparing a daidzein nanoparticle delivery carrier, belonging to the field of food processing technology. Using zein, carrageenan, and sodium alginate as raw materials, this invention utilizes ultrasound technology to construct a novel nanoparticle delivery carrier: zein nanoparticles loaded with a carrageenan / sodium alginate bistabilized layer, thereby improving the stability and bioavailability of daidzein nanoparticles. The advantages of this invention lie in the simplicity and environmental friendliness of the antisolvent precipitation method and ultrasound technology. The prepared composite nanoparticles possess stable physicochemical properties, small particle size, relatively concentrated particle size distribution, and high bioavailability. This invention enhances the practical application value of daidzein-loaded proteoglycan nanoparticles in the food processing industry and provides a theoretical basis for the application of daidzein nanoparticle oral delivery systems.

The present application relates to the engineered microorganism capable of synthesizing calycosin or its derivative and its construction method and application. The present application carries out metabolic engineering modification on the Saccharomyces cerevisiae capable of synthesizing daidzein, screens the isoflavone-3'-hydroxylase capable of efficiently converting the intermediate product into calycosin, optimizes the enzyme combination, obtains the engineered Saccharomyces cerevisiae capable of efficiently synthesizing calycosin and calycosin glycoside, and realizes the calycosin glycoside yield of 47.60 mg / L after 72 h fermentation through the mode of adjusting metabolic flux and optimizing culture conditions, which lays the foundation for the industrialized production of calycosin glycoside.

The invention discloses a method for efficiently extracting active ingredients from radix puerariae, the active ingredients comprise 3 '-hydroxypuerarin, the extraction method comprises the following steps: S1, raw material pretreatment: selecting dried radix puerariae tuberous roots, crushing and sieving with a 40-60-mesh sieve, and the invention relates to the technical field of plant active ingredient extraction. According to the method for efficiently extracting the active ingredients from the radix puerariae, through the synergistic effect of composite solvent extraction and gradient purification, synchronous and efficient enrichment is achieved on trace 3 '-hydroxypuerarin and a compound A in the radix puerariae, the extraction rate is larger than or equal to 15%, the extraction rate of the compound A is larger than or equal to 85%, and the extraction rate is increased by three times or above compared with a traditional single solvent extraction method; the total purity of the active ingredients is greater than or equal to 95%, the problems of low extraction rate of the target ingredients and difficult separation of impurities in the traditional process are solved, and the pharmacological activity of the 3 '-hydroxy puerarin and the compound A are synergistically enhanced, so that the application requirements of high-purity plant source active ingredients are met, and a high-quality raw material is provided for subsequent development.

The invention discloses a preparation method of zein-starch Pickering emulsion loaded with daidzein and relates to the technical field of emulsion preparation, waxy corn starch is subjected to enzymolysis through pullulanase and is applied to emulsion construction, and the emulsion with good stability is researched to realize efficient loading. The invention also discloses a zein-short amylose composite nanoparticle, a preparation method of a Pickering emulsion stabilized by the composite nanoparticle, and the Pickering emulsion stabilized by the composite nanoparticle. The Pickering emulsion with stable composite nanoparticles prepared by the invention has the characteristics of high stability and high safety, and provides a substitute with a good application prospect for the transportation of a bioactive substance daidzein.

The invention provides application of lactobacillus brevicornu and a fermentation composition thereof in hangover alleviating and liver protection, and relates to the technical field of biological fermentation, the lactobacillus brevicornu is lactobacillus brevicornu, the strain number of the lactobacillus brevicornu is KMHD10-222, and the registration number of the lactobacillus brevicornu in the China General Microbiological Culture Collection Center is CGMCC No. 32358. Traditional Chinese medicine substrates can be fermented, and the hangover-alleviating and liver-protecting effects of fermentation products are enhanced. The content of rare ginsenoside, total polysaccharide, total flavone, total phenol, puerarin, daidzein and dihydromyricetin of the lactobacillus brevis KMHD10-222 fermentation substrate extracting solution is increased, the effects of promoting ethanol metabolism and improving the alcoholic liver disease can be improved, and the effects of dispelling the effects of alcohol and protecting the liver are achieved.

The application provides a preparation method and content determination method of Zhizi Chi Tang reference standard for treating Alzheimer's disease, and belongs to the field of traditional Chinese medicines.The preparation method comprises the following steps: a, weighing and proportioning raw medicinal materials;b, soaking gardenia with water, decocting, then adding light soybean, decocting for 30-60 minutes, filtering, and taking water extract;b, freezing and drying the water extract of step b.The application also provides the Zhizi Chi Tang reference standard prepared by the preparation method and the content determination method.The application screens out daidzein, genistein and quercetin as the effective components of Zhizi Chi Tang for treating Alzheimer's disease, establishes the preparation method of the Zhizi Chi Tang reference standard, establishes the content determination method by using high performance liquid chromatography, and applies the method to the product quality control of Zhizi Chi Tang for treating Alzheimer's disease, so as to ensure the effectiveness and stability of the product, and the method is simple and convenient, good in stability, high in precision, good in reproducibility, and easy to master.

The application belongs to the technical field of biological medicine, and proposes a use of a pterostilbene derivative and a preparation of a medicine for treating and / or preventing diseases caused by oxidative stress. The chemical structure of the pterostilbene derivative is shown as formula (I)-(III). The scavenging effect of the obtained derivative on DPPH·, O2 ‑ · and ABTS + · free radicals and its total reducing capacity are detected respectively. In terms of DPPH· and total reducing capacity, it is found that the scavenging capacity of some derivatives is equivalent to that of pterostilbene or vitamin C. Derivatives 2a, 4a, 4c, 4e, 4h, 4i and 4m-4p have the same scavenging effect on O2 ‑ · free radicals as pterostilbene or vitamin C; the scavenging effect of derivative 2b on ABTS + · is equivalent to that of vitamin C and is superior to that of pterostilbene. The pterostilbene derivative has potential application prospects in the preparation of antioxidants.

To provide a tablet containing isoflavones that achieves both hardness and compressibility. [Solution] The tablets of the present invention contain (A) isoflavones, (B) excipients, and (C) lubricants. It is preferable that (A) isoflavones contain daidzein and genistein. It is also preferable that the mass ratio of daidzein to genistein is genistein:daidzein = 1:0.5 to 4.5. Furthermore, it is also preferable that the tablets contain (D) a fluidizing agent.

The application provides a liquid chromatography-mass spectrometry method for detecting phytosterol content in dried blood spots, and belongs to the technical field of detection.The application simplifies a sampling process, uses a disposable blood taking needle and a blood taking card for sampling, replaces traditional serum or plasma samples with obtained dried blood spots, punches a sampling area of the dried blood spot sample through a puncher with a diameter of 3.2 mm, takes three dried blood spot samples, extracts seven kinds of sterol substances contained in the dried blood spot samples through n-hexane, adopts liquid chromatography-mass spectrometry, carries out detection and quantification through chromatographic separation and a mass spectrometry detector, realizes rapid and accurate determination of cholesterols, beta-sitosterols, daidzein, campesterol, dihydrocholesterol, dehydrocholesterol and 7-alkenyl cholestanol, and the method is simple in operation, accurate in quantification, and has a recovery rate of 92.4%-96.7%.

The invention provides a tobacco additive composition for relieving respiratory tract discomfort symptoms. The tobacco additive composition comprises daidzein and naringenin. The tobacco additive disclosed by the invention has unique characteristics. When the tobacco is ignited, the daidzein and the naringenin are not changed by combustion, but are directly converted into gaseous states, and are inhaled into the respiratory tract of a human body along with smoke generated by combustion of the tobacco. The characteristic specially exerts positive effects on chronic pharyngitis and pharyngolaryngitis, not only can relieve diseases and prevent diseases, but also can assist in treatment, is free of any complications after being used for a long time, and provides a solid guarantee for health and safety of a user. In the aspect of use experience, the additive enables tobacco products to be better in taste, and the additive is easily accepted by vast smokers.

The invention provides a preparation method and a content determination method of a gardenia and fermented soybean decoction standard substance for treating Alzheimer's disease, and belongs to the field of traditional Chinese medicines. The preparation method comprises the following steps: a, weighing the raw material medicines according to the weight ratio; b, adding water to soak and decoct fructus gardeniae, then adding fermented soybean, decocting for 30-60 minutes, filtering, and taking a water extract; and c, freeze-drying the water extract obtained in the step b. The invention also provides the reference standard substance of the cape jasmine and fermented soybean soup obtained by the preparation method and a content determination method. Effective components including daidzein, genistein and quercetin of the gardenia and fermented soybean decoction for treating the Alzheimer's disease are screened out, a preparation method of a gardenia and fermented soybean decoction reference sample is established, a content determination method is established by adopting high performance liquid chromatography, the method is used for product quality control of the gardenia and fermented soybean decoction for treating the Alzheimer's disease, effectiveness and stability of the product are guaranteed, and the method is suitable for popularization and application. The method is simple, convenient, good in stability, high in precision, good in reproducibility and easy to master.

The invention discloses application of lactobacillus plantarum CCFM1283 in conversion of curcumin and isoflavone and application of lactobacillus plantarum CCFM1283 in relieving anxiety and resisting fatigue, and belongs to the technical field of microorganisms. According to the invention, the screened plant lactobacillus CCFM1283 can promote the application of curcumin in function exertion, which is specifically embodied in that: (1) curcumin can be converted into tetrahydrocurcumin with higher activity, and in addition, pueraria isoflavone glycoside can be efficiently converted into daidzein and genistein with higher biological activity; (2) the movement time of the machine body can be prolonged; (3) the anti-oxidation (SOD, MDA and GSH-PX) capability of the body can be improved; (4) body neurotransmitter (DA, 5-HT, NE) unbalance can be slowed down; and (5) the expression of anti-fatigue related genes (Nrf2, HO-1, SOD1 and GPX1) of an organism can be improved. Therefore, the application of the plant lactobacillus CCFM1283 to the curcumin-containing product has a huge application prospect.

To provide an effective isoflavone absorption promoter which can be safely and daily taken.SOLUTION: To provide a vitamin B1 and / or an absorbefacient for isoflavones containing the vitamin B6. The isoflavone is preferably daidzein and / or genistein. It is also preferable to contain vitamin B1 and vitamin B6. It is also preferable that the content ratio of the vitamin B1 to the vitamin B6 is 1:3 to 3:1 by mass ratio. It is also preferably for oral use.SELECTED DRAWING: None

The invention provides a method for measuring the content of active ingredients in gardenia-rhubarb decoction. The method comprises the following operation steps: 1) preparing a reference solution; (2) preparing a test solution; and (3) detecting by adopting an HPLC (High Performance Liquid According to the method for determining the content of the active ingredients in the gardenia-rhubarb decoction, the reference sample of the rhubarb decoction is obtained on the basis of the original record of Jingui Litu and is used for constructing the method for detecting the active ingredients in the gardenia-rhubarb decoction; according to the method, high-resolution detection of seven key functional components including genipin gentiobioside, geniposide, narirutin, hesperidin, daidzein, emoditin-7-O-beta-D-glucoside and genistein is realized, the specificity is high, the repeatability is good, a reliable technical support is provided for quality standard improvement and clinical precise medication of the gardenia-rhubarb decoction, and the method has a wide application prospect. The method has important significance on guaranteeing the stability of the gardenia-rhubarb decoction reference sample.

The invention relates to the technical field of agricultural biology, in particular to a P450 enzyme mutant, a method for improving C3'hydroxylation activity of P450 enzyme and a method for generating 3 '-ODI through whole cells. The cytochrome P450 enzyme mutant disclosed by the invention can selectively catalyze the daidzein to generate a C3'hydroxylation reaction to generate corresponding 3 '-ODI, has excellent selectivity and relatively high yield, and provides an efficient and reliable catalytic tool for the C3' hydroxylation reaction of the daidzein. The P450 enzyme mutant, a glucose dehydrogenase driven NAD (P) H regeneration module and a redox partner system Fdx / FdR are subjected to multi-enzyme co-expression, a cofactor self-circulating single plasmid four-enzyme co-expression system is successfully constructed, and the method is mild in reaction condition and low in catalyst cost and is an ideal scheme for preparing 3 '-ODI.

The invention relates to a method of modulating production of a metabolite in an actinomycetes bacterium comprising increasing the expression or activity of the Sporulation and Antibiotic Production Related gene A (SarA. SCO4069) polypeptide to modulate production of the metabolite in the actinomycetes bacterium. In one embodiment, the metabolite is valinomycin, daidzein, fluvirucin, demethyllydicamycin, or TPU-0037 analogues A. C and D.

Soybean genistein base alkene monomer, soybean genistein base thermosetting resin and preparation method thereof belong to the field of biomass polymer material preparation, soybean genistein, bromine propylene and phase transfer catalyst are added into a reaction system, then alkali and solvent are added, after mixing uniformly, reaction is carried out at room temperature, after reaction is completed, soybean genistein base alkene monomer is obtained through filtration and purification. Then, soybean genistein base alkene monomer and thiol curing agent are mixed according to a certain proportion, 4-dimethylaminopyridine is added and mixed uniformly, heating, degassing, pouring into a mold, and after ultraviolet light curing process, soybean genistein base thermosetting resin is obtained. The soybean genistein base thermosetting resin can be obtained by thiol-alkene click chemistry reaction of soybean genistein base alkene monomer and thiol, respectively, and the mechanical properties of the biomass thermosetting resin can be controlled. The soybean genistein base thermosetting resin has very low dielectric constant, and has wide application prospect in the field of electronic packaging materials.

The invention provides a UPLC (Ultra Performance Liquid Chromatography) content detection method of cape jasmine and fermented soybean soup without negative sample interference, which comprises the following steps: a, preparation of a test solution: taking a cape jasmine and fermented soybean soup sample, dissolving with a solvent, and extracting to obtain a to-be-detected solution; b, measuring the liquid to be measured by adopting an ultra-high performance liquid chromatography, and measuring the contents of daidzein and genistein; the conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; methanol is used as a mobile phase A, a 1% glacial acetic acid solution is used as a mobile phase B, and gradient elution is carried out. According to the method, the total amount of daidzein and genistein in the reference sample of the gardenia and fermented soybean decoction can be effectively controlled, so that the uniformity, stability and controllability of the preparation quality of the reference sample of the gardenia and fermented soybean decoction are ensured, the interference of a negative sample is avoided, and the basic clinical curative effect of the reference sample of the gardenia and fermented soybean decoction is ensured.

This invention relates to the field of daidzein synthesis, specifically to a daidzein, its preparation method, and its applications. This invention synthesizes a novel daidzein by co-expressing genes AtPAL2, GmCHS8, GmCHR5, GmCHI1B1, GmIFS1, GmHID, and AtMYB60 (genes for daidzein synthesis), downstream daidzein synthesis genes GmI2'H, GmIFR, GmVR, GmPTS1, GmD6aH, and GmG2DT, and the daidzein synthase gene GmGS3e / 7 in *Nicotiana benthamiana* mediated by *Agrobacterium*, thus paving the way for heterologous daidzein biosynthesis. This daidzein effectively inhibits fungal activity, showing good inhibitory effects against *Phytophthora sacchari* and *Phytophthora xanthoides*, and has broad application prospects in green pest control and food development.

This invention discloses a compound microbial preparation for controlling root-knot nematodes and promoting plant growth, as well as its preparation and application. Addressing the technical problems of existing biocontrol agents, such as lack of target optimization based on root-knot nematodes, unclear metabolite profiles, poor synergistic effects, and unstable insecticidal and growth-promoting properties, this invention screens *Polyspora pinki* and *Bacillus belye* for co-culture, and through inter-bacterial interactions, directionally enriches synergistic metabolites such as daidzein, isopentenyl adenine, and melatonin. When applied to the rhizosphere, this preparation reduces the root-knot index of cucumbers by ≥40% and increases the fresh weight of aboveground parts by ≥25%, significantly superior to single-strain or physical mixture systems. Furthermore, the product is flexible in form, possessing both viable bacterial colonization ability and metabolite stability; its activity retention rate is >80% after 90 days of storage at 35℃, making it suitable for various application methods such as root irrigation and seed coating, providing a highly efficient and stable technical solution for the green control of root-knot nematodes.

To provide an isoflavone-containing beverage in which the characteristic flavor of isoflavones is effectively masked. [Solution] The beverage of the present invention contains isoflavones and excipients. Preferably, the isoflavones contain daidzein and genistein. Preferably, the mass ratio of daidzein to genistein is genistein:daidzein = 1:0.5 to 4.5. Preferably, the beverage further contains one or more selected from barley grass and protein.

The application discloses an application of Lachnospiraceae bacterium and / or daidzein in radiation-induced intestinal injury. The study finds that the intestinal flora-derived Lachnospiraceae bacterium (Lachnospiraceae bacterium BNCC354474) has a significant protective effect on radiation-induced intestinal injury, can remodel the intestinal flora structure after irradiation, and restore the flora homeostasis. Meanwhile, through the target regulation of the inflammation pathway by the metabolite daidzein, the radiation-induced intestinal inflammation is relieved. The application clarifies the role and application potential of Lachnospiraceae bacterium and its metabolite in the regulation of radiation-induced intestinal injury, and provides a new microecological strategy for the intervention treatment of patients with radiation-induced intestinal injury.

This invention relates to the field of biotechnology, specifically to a biosynthetic method for the synthesis of daidzein precursors and daidzein based on daidzein catalysis. This invention synthesizes daidzein precursors glyceollidinⅠ (GD1) or glyceollidinⅡ (GD2) by co-expressing the genes AtPAL2, GmCHS8, GmCHR5, GmCHI1B1, GmIFS1, GmHID and AtMYB60, which are involved in daidzein synthesis, along with downstream daidzein genes GmI2'H, GmIFR, GmVR, GmPTS1, GmD6aH and GmG4DT, or GmI2'H, GmIFR, GmVR, GmPTS1, GmD6aH and GmG2DT, in *Nicotiana benthamiana* mediated by *Agrobacterium*. Furthermore, it co-expresses any one or a combination of several of the daidzein synthase genes GmGS1a, GmGS1b, GmGS2a, GmGS2b, GmGS3 / 7, and GmGS3e. This invention utilizes the Benedictine platform, based on daidzein, a key precursor in the biosynthesis of highly accumulated isoflavones, to reconstruct the biosynthetic pathways of the direct precursors of daidzein, GD1 and GD2. It elucidates the daidzein biosynthetic pathway, paving the way for heterologous daidzein biosynthesis and laying a scientific theoretical and technological foundation for the large-scale industrial production and medicinal function research of antibacterial daidzein.

The application discloses a Leuconostoc mesenteroides subsp. mesenteroides strain for promoting the efficacy of puerarin and application thereof, and belongs to the technical field of microorganisms. The Leuconostoc mesenteroides subsp. mesenteroides strain CCFM1282 screened by the application can convert puerarin into various active small molecular substances containing daidzein; the cell proliferation ability and the antioxidant capacity of HepG2 alcoholic cell damage can be improved after fermentation; the ability of liver cells to cope with alcohol damage can be improved by the compound pretreatment of puerarin and the strain; and the Leuconostoc mesenteroides subsp. mesenteroides strain CCFM1282 can significantly improve the efficacy of puerarin for dispelling alcohol. The Leuconostoc mesenteroides subsp. mesenteroides strain CCFM1282 is used for converting puerarin and significantly improving the efficacy of dispelling alcohol, and has great application prospect.

The application belongs to the field of biosynthesis and particularly relates to a Saccharomyces cerevisiae engineering bacterium for producing bakuchiol, a construction method and application thereof. The Saccharomyces cerevisiae engineering bacterium comprises a DMAPP production pathway, a naringenin synthesis pathway, a daidzein synthesis pathway and a bakuchiol synthesis pathway. The DMAPP production pathway is expressed by mvaE, mvaS, ERG12, ERG8, ERG19 and IDI1 in the Saccharomyces cerevisiae strain WAT11. The naringenin synthesis pathway is expressed by PAL, 4CL, CHS and CHI in the Saccharomyces cerevisiae strain with increased dimethylallyl pyrophosphate. The daidzein synthesis pathway is expressed by CHR, IFS and HID in the Saccharomyces cerevisiae strain with naringenin synthesis. The bakuchiol synthesis pathway is expressed by PT and OC in the Saccharomyces cerevisiae strain with daidzein synthesis.

Composition of a nanostructured lipid carrier system for the effective treatment of arthritis, the composition comprising: (a) Daidzein as a pharmaceutically active ingredient; (b) a solid lipid selected from glyceryl monostearate, stearic acid, cetyl palmitate or combinations thereof; (c) a liquid lipid selected from oleic acid, caprylic / capric triglyceride, medium-chain triglycerides or mixtures thereof; (d) one or more surfactants selected from Tween 80, Poloxamer 188, Span 20 or combinations thereof; (e) one or more co-surfactants selected from propylene glycol, polyethylene glycol, ethanol or combinations thereof; (f) an aqueous phase suitable for the formation of a stable nanodispersion; wherein the nanostructured lipid carrier has a particle size between 50 and 300 nm, a polydispersity index ≤ 0.5 and an inclusion efficiency of at least 70% and wherein the composition is formulated to provide improved skin permeation and delayed release of daidzein for an anti-inflammatory and anti-arthritic effect.

The application provides a bio-based Mannich base, a preparation method thereof, an application thereof, and a TDI curing agent prepared by using the bio-based Mannich base as a catalyst. The bio-based Mannich base is obtained by a Mannich reaction of natural phenol, dimethylamine and formaldehyde, wherein the natural phenol is one or more selected from the group consisting of sesamol, catechin, theaflavin, daidzein and procyanidin. The Mannich base catalyst prepared by using the natural phenol in the application improves steric hindrance, and the reaction selectivity of small molecule monomers can be improved through the steric hindrance effect. The content of free TDI monomers in the TDI curing agent can be reduced by adding the catalyst at one time, so that the free monomers in the prepared TDI curing agent are less than 0.5% advantageously.

This invention discloses a laccase mutant and its application in the oxidative conversion of daidzein to prepare polyflavonoids. This invention uses *Coprinus comatus* (Grey-capped Coprinus) as a starting material. Coprinopsis cinerea Using laccase Lcc5 as the starting enzyme, molecular docking was used to analyze its positional structure and design mutations. The mutant gene was synthesized, and a recombinant expression engineered bacterium containing the mutant gene was constructed. After induction of expression in the engineered bacterium containing the mutant gene, the Lcc5 mutant Lcc5-T485E was obtained through isolation and purification. This mutant showed improved specific enzyme activity and ethanol tolerance, increasing the conversion rate of daidzein by approximately 1.26 times under the same enzyme activity. The antioxidant activity, enzyme-inhibiting activity, and antibacterial activity of the obtained transformation product were also significantly enhanced. This mutant enzyme has potential application value in the oxidative conversion of daidzein to improve its biological value.

A topical sunscreen composition consisting of daidzein-loaded liposomes embedded in a cosmetically acceptable sunscreen base, wherein the liposomes have a mean vesicle size of approximately 198 nm and a polydispersity index of approximately 0.17 and are configured to increase the in vitro sun protection factor (SPF) and skin deposition of daidzein compared to a non-liposomal formulation.